CN107079707A - One kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content - Google Patents
One kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content Download PDFInfo
- Publication number
- CN107079707A CN107079707A CN201710474319.1A CN201710474319A CN107079707A CN 107079707 A CN107079707 A CN 107079707A CN 201710474319 A CN201710474319 A CN 201710474319A CN 107079707 A CN107079707 A CN 107079707A
- Authority
- CN
- China
- Prior art keywords
- romaine lettuce
- concentration
- lettuce
- stress
- spray
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
- A01G2013/004—Liquid mulch
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
GA is utilized the invention provides one kind3The method for significantly reducing Leaf lettuce cadmium content, it comprises the following steps：After Leaf lettuce seedling is ripe, with the GA that concentration is 1~10 μm of ol/L3The aqueous solution carries out foliage-spray, and the amount of spraying is dripped for degree with blade face, sprayed 1 time, spray altogether 3 times every 2 days.Present invention facilitates the growth of the lower romaine lettuce of Cd stress, plant height has been obviously improved；Alleviate suppression of the Cd stress to romaine lettuce photosynthetic rate；Injury of the oxidative stress of Cd inductions to cell membrane is alleviated, romaine lettuce MDA contents and electrolytic leakage is reduced；Romaine lettuce overground part Cd contents and nitrate content are reduced, the quality of romaine lettuce is improved.
Description
Technical field
The invention belongs to growing vegetables technical field, and in particular to one kind utilizes GA3Significantly reduce Leaf lettuce cadmium content
Method.
Background technology
According to the issue of geologic survey office of China《Chinese arable land GEOCHEMICAL SURVEY reports (2015)》It has been shown that, China has 232
Ten thousand hm2Serious pollution or exceeded arable land, account for 16.1% always ploughed in heavy metal.On April 17th, 2014 Environmental Protection Department and state
Soil resource portion, which combines, discloses national Investigation of Soil Pollution publication, it is indicated that China's soil environment situation makes people worried, the huge sum of money such as Cd
Belong to pollution problem relatively to protrude, in terms of pollution distribution situation, soils in south china heavy contamination, soils of north China pollutes relatively
Gently, southwest, variable charge soil heavy metals exceeding standard scope are larger.
Cd content distributions show the situation gradually risen from northeast to southwest, from northwest to southeastern direction, and Cd points position is super
Mark rate is 7.0%, and accounting is maximum in contaminated soil, wherein, light contamination, slight pollution, intermediate pollution, the ratio of serious pollution
Example is respectively 5.2%, 0.8%, 0.5%, 0.5%.The Cd contents of contaminated soil are 2.5~23.0mg/kg, seriously polluted area top layer
The Cd contents of soil are higher by tens times of even more highs of subsoil.
According to Ministry of Agriculture's environmental monitoring system detection data display, compared with national standard, Cd content overproofs rate in vegetables
Up to 23.5%.Analyzed by heavy metal element in the edible part of 9 kinds of vegetables, 152 samples to In Chengdu, find Cd
It is one of main pollution-producing in Vegetables from Chengdu, in detection sample, Cd exceeding standard rate is 29.4%, and highest is exceeded
5.6 again.
As can be seen here, current China Cd pollution situations allow of no optimist, and oneself threatens food security and health, must cause
Pay much attention to.
Absorption of the different plants to different heavy metals has certain rule, it is however generally that, vegetables edible part absorbs heavy metal
Ratio be more than cereal crops, vegetables heavy metal Cd of the same race enrichment is more than other heavy metal elements.But suction of the vegetables to Cd
Receive different because of vegetable species, kind and organ difference.
Absorption of the plant to mineral nutrition has genotypic difference, and absorption, accumulation of the same vegetables to Cd also have gene
Type difference, this species diversity is to cause one of major reason of different vegetable Cd content differences.
The research such as Li Bowen is found：Eggplant, sponge gourd, tomato, capsicum etc. are the low accumulation type vegetables of Cd；Ternip, cauliflower, lettuce
Lettuce, shallot etc. are moderate accumulation type；Celery, fennel, caraway, crowndaisy chrysanthemum are severe accumulation type；Chinese cabbage, rape belong to pole severe accumulation
Type.Horse studies the pollution situation of Cd in several vegetables toward school etc., as a result shows, Cd accumulation law is celery in different vegetables
＞ leek ＞ asparagus lettuce ＞ pakchoi ＞ cauliflower ＞ cucurbita pepos, cucumber, green pepper, celery and asparagus lettuce have stronger absorption energy to Cd
Power, exceeded most serious.
There is also larger difference for the absorption of the different cultivars heavy metal of vegetables of the same race.By taking carrot as an example, Nairobi pairs
Cd uptake is 1.215mg/kg, and Amsterdam uptake is then 2.521mg/kg, the different product of other 5 kinds of vegetables
There is also otherness absorption to cadmium ion for kind.Cd contents in the pakchoi cauline leaf of research 13 kinds of different genotypes of discovery such as Wang Songliang
Difference is very big.Under identical Cd concentration, up to 4.3 times of Cd contents difference between different varieties of Brassica campestris, and external world's Cd concentration is higher,
Differences are bigger.
At present, for the research of the plant growth under heavy metal stress, its growth is mainly adjusted in plant by studying
Material carry out.The plant growth regulator being currently known is people consider when improving stress resistance of plant it is main because
Element.But, existing known plants growth regulator is when adjusting stress resistance of plant, and selectivity is poor, it is difficult to formed for many
The unified treating method of stress situation is planted, or even the treating method of Different stress situation can also be far from each other.For the heavy metal side of body
For compeling, because the stress is different from the typically stress such as high temperature, high salt, when handling heavy metal stress, vegetables are not only improved
Antioxidation activity and plant growth performance, what is more important needs to reduce accumulation of the heavy metal in vegetables, can be only achieved
The purpose of vegetable cultivation.
At present, the research of the heavy metal stress (particularly Cd stress) for solving romaine lettuce is also relatively fewer, and reason is
At present for mechanism not yet thoroughly research of the heavy metal for the influence of romaine lettuce, corresponding treating method is still among groping.
《Exogenous Nitric Oxide is to peanut under Cd stress and the relieving effect and its study mechanism of Growth of Lettuce》By to life
Dish applies exogenous carbon monoxide and significantly reduces cadmium content of the production under Cd stress.
《Relieving effect of the salicylic acid to lettuce growth of seedling and oxidative stress under Cd stress》It has studied for lettuce sativa
Using relieving effect of the salicylic acid for Cd stress.But this report mentions salicylic acid in for probing into terms of Cd accumulation
There is facilitation from root system to the transport on top for Cd.On its Research foundation, it is possible to using exogenous salicylic acid to drop
Low cadmium content is unhelpful.
Therefore, the cadmium content in romaine lettuce under Cd stress how is reduced, is urgently for romaine lettuce planting technology field
The technical problem of research.
The content of the invention
The technical barrier faced for prior art, GA is utilized it is an object of the invention to provide one kind3Significantly reduce leaf
With the method for lettuce cadmium content, it comprises the following steps：It is 1~10 μm of ol/L with concentration after Leaf lettuce seedling is ripe
GA3The aqueous solution carries out foliage-spray, and the amount of spraying is dripped for degree with blade face, sprayed 1 time, spray altogether 3 times every 2 days.
It is preferred that, the GA3The concentration of the aqueous solution is 2.5~5 μm of ol/L.
It is furthermore preferred that the GA3The concentration of the aqueous solution is 2.5 μm of ol/L.
It is preferred that, utilizing GA3When the aqueous solution carries out foliage-spray, spraying time is early morning.
It is preferred that, spray GA the 1st time3The time of the aqueous solution is the 3rd day after Leaf lettuce seedling maturation.
Leaf lettuce used is glass romaine lettuce.
Beneficial effects of the present invention：
1st, present invention facilitates the growth of the lower romaine lettuce of Cd stress, plant height has been obviously improved it；
2nd, the present invention alleviates suppression of the Cd stress to romaine lettuce photosynthetic rate；
3rd, the present invention alleviates injury of the oxidative stress of Cd inductions to cell membrane, reduces romaine lettuce MDA contents and electrolysis
Matter permeability；
4th, present invention reduces romaine lettuce overground part Cd contents and nitrate content, the quality of romaine lettuce is improved.
Embodiment
The present invention is specifically described below by embodiment, it is necessary to it is pointed out here that be following examples be use
It is further detailed in the present invention, it is impossible to be interpreted as limiting the scope of the invention, the field is skilled in technique
Some nonessential modifications and adaptations that personnel are made according to foregoing invention content, still fall within protection scope of the present invention.
Embodiment 1
Selection is in the same size, and (from Guangdong Kenong Vegetables Seed Industry Co., Ltd., kind is the romaine lettuce seed of full seed for purchase
The common glass romaine lettuce in Sichuan province), 10min is sterilized in 10% (m/m) hydrogenperoxide steam generator, is then cleaned with ultra-pure water,
Uniformly it is positioned in the culture dish for being lined with filter paper, keeps adequate water, vernalization is carried out in 20 DEG C of artificial incubators.Seed shows money or valuables one carries unintentionally
After 0.5cm, it is sowed in the enamel pallet equipped with clean river sand and carries out sand culture.According to upgrowth situation, daily timely and appropriate discovery to
Sha Jizhong sprays 1/2Hoagland nutrient solutions, and incubator is set to 23 DEG C/18 DEG C (day night), illumination 14h.Treat that seedling 4 is true
When leaf is fully deployed, the consistent strong sprout of selection growing way is transplanted to 10cm × 10cm (bottoms equipped with clean river sand (playing fixation)
× high) in plastic flowerpot (flowerpot bottom is lined with gauze, prevents river sand from spilling), per 2 plants of basin, and flowerpot is placed in high 8cm modeling
The Hoagland pancebrins containing Cd are filled in charging tray, in disk, Cd concentration is 10mg/L (with CdCl2·2.5H2O2Add),
One time of nutrition liquid is changed per 2d.After the romaine lettuce seedling transplanted survives (2d) completely, with the GA of various concentrations3Solution is in 9:00 spray
Cloth romaine lettuce blade, is dripped for degree with blade, sprayed 3 times altogether every 2d once.To be cultivated without Cd pancebrins, spray clear water
Romaine lettuce be control (CK), the pancebrins of Cd containing 10mg/L cultivation, the romaine lettuce of spray clear water is 1 (CK1) of control.Setting 4 is dense
Gradient (1 μm of ol/L, 5 μm of ol/L, 10 μm of ol/L, 20 μm of ol/L) is spent, each processing is repeated 3 times.
Spraying GA3After 30d, the sampling of romaine lettuce maturity period.Whole strain is determined after growth and morphological index, (edible by overground part
Portion), root preserve fresh sample and dry sample respectively.After fresh sample is sampled with liquid nitrogen, -80 DEG C preserve in case physiology refers in ultra low temperature freezer
The measure of mark and the index of quality；Dry sample is contained with being dried after 105 DEG C of fixing 15min of baking oven with 75 DEG C to constant weight after crushing for Cd
The measure of amount.
Growth indexes and biomass：
Plant height, root length are measured using millimeter graduated scale, and stem slightly uses vernier caliper measurement.
Biomass：After the whole strain of plant sample is cleaned with running water, then rinsed repeatedly with deionized water, wipe dry.By romaine lettuce
It is divided into overground part and root, fresh weight is weighed with electronic balance.Then overground part and root system are dispensed, with 105 DEG C of fixings of baking oven
15min, 75 DEG C of drying weigh dry weight to constant weight.
Photosynthetic pigments and photosynthetic parameters：
Contents of Photosynthetic Pigments uses acetone ethanol mixed liquor extraction[84]Determine.
Choose the 2-3 piece functional leafs of romaine lettuce, with the portable photosynthetic instrument of LI-6400XT (LI-COR, the U.S.) determine Pn,
Gs, Tr, Ci and Ls, it is 1000 μm of olm to survey the endogenous light intensity of timing setting-2·s-1, CO2Concentration is 400 μ lL-1, temperature is
25℃。
Cell leakage, osmotic adjustment and root activity：
MDA contents are determined using thiobarbituricacidα- method；Proline content uses sulfosalicylic acid method；Root activity is used
Triphenyltetrazolium chloride (TTC) method is determined.
Cell leakage uses Means of Electrical Conductivity：Weigh 0.2g romaine lettuce samples to be put into 20mL deionized waters, normal temperature vibration
30min, determines electrical conductivity of solution (A)；Then romaine lettuce sample and deionized water one are arised from and boils 20min at 100 DEG C, using going
Ionized water is adjusted to heating precursors product, determines electrical conductivity of solution (B).Cell leakage (%)=electrical conductivity (A)/electrical conductivity (B)
× 100%
Activities of antioxidant enzymes：
SOD activity uses nitroblue tetrazolium (NBT) method；POD activity uses guaiacol method；CAT activity uses UV absorption
Method.
The index of quality：
Soluble protein content uses Coomassie brilliant G-250 decoration method；Soluble sugar content is adopted
Use anthrone colorimetry；
Nitrate nitrogen content uses salicylic acid colorimetry.
Cd contents：
By romaine lettuce dry sample HNO3-HClO4Digestion, uses ICP-8000 inductive coupling plasma emission spectrographs
(PerkinElmer, the U.S.) is determined.
Data processing：
All data are arranged using the softwares of Excel 2010；SPSS 20.0 carries out variance analysis and correlation analysis,
Duncan duncan's new multiple range methods carry out Multiple range test.Root/shoot ratio=weight of root system/overground part dry weight, Ls=1-Ci/Ca, transhipment system
Number=overground part Cd contents/root system Cd contents.
External source GA3The influence of lower Growth of Lettuce is coerced Cd
From table 1 it follows that in Cd contaminated soils, romaine lettuce foliage-spray GA3, promote the elongation growth and increasing of romaine lettuce
Girth growth.Spray GA3It has been obviously improved the plant height of the lower romaine lettuce of Cd stress.GA3It is raw between processing of the concentration for 2.5~10 μm of ol/L
Dish difference in plant height is not notable, is all remarkably higher than other processing.Under Cd stress, with GA3The rise of concentration, after romaine lettuce stem slightly first increases
Reduction, in GA3Highest, (P ＜ 0.05) higher than CK1 by 14.79% when concentration is 2.5 μm of ol/L.Spray GA3Concentration be 1 μm of ol/L and
During 10 μm of ol/L, though the thick value of romaine lettuce stem is higher than CK1, with CK1 without significant difference.Under Cd stress, GA is sprayed3Each processing life afterwards
Dish root length, without significant difference, works as GA with CK13Concentration is most long when being 2.5 μm of ol/L, is 10.06cm.
Table 1GA3The influence of lower Growth of Lettuce is coerced Cd
External source GA3The influence of lower romaine lettuce biomass is coerced Cd
With GA3The rise of concentration, the lower romaine lettuce overground part fresh weight of Cd pollutions, dry weight and weight of root system first increase to be reduced afterwards.1
μm ol/L and 2.5 μm of ol/L GA3Though the romaine lettuce overground part fresh weight of processing is higher than CK1 by 2.26% and 3.56% respectively, and CK1
Without significant difference.Spray GA3When concentration is 5 μm of ol/L and 10 μm of ol/L, romaine lettuce overground part fresh weight is reduced respectively compared with CK1
5.83% (P ＞ 0.05) and 8.75% (P ＜ 0.05).Under Cd stress, GA is sprayed3Romaine lettuce overground part dry weight becomes with overground part fresh weight
Change trend is consistent, in GA3When concentration is 2.5 μm of ol/L, romaine lettuce overground part dry weight highest is higher than CK1 by 4.62%.Spray high concentration
The GA of (>=5 μm of ol/L)3Afterwards, the lower romaine lettuce overground part dry weight reduction of Cd pollutions is exacerbated, works as GA3Concentration is minimum when being 10 μm of ol/L,
(P ＜ 0.05) lower than CK1 by 6.94%.Under Cd stress, weight of root system maximum, which is appeared in, sprays GA3Concentration is 2.5 μm of ol/L
Processing, minimum value appears in GA3When concentration is 10 μm of ol/L.Spray 1~5 μm of ol/L GA3Afterwards, romaine lettuce root/shoot ratio is more than CK1,
As the GA for spraying 10 μm of ol/L3Afterwards, romaine lettuce root/shoot ratio is reduced, lower than CK1 by 4.51%.
Table 2GA3The influence of lower romaine lettuce biomass is coerced Cd
External source GA3The influence of lower romaine lettuce Contents of Photosynthetic Pigments is coerced Cd
CK1 chlorophyll as, chlorophyll b and total chlorophyll content are lower than CK by 16.71% (P ＜ 0.05) respectively, 17.57% (P
＜ 0.05) and 16.90% (P ＜ 0.05).Under Cd stress, romaine lettuce blade carotenoid content is slightly lower compared with CK, but without significance difference
It is different.Spray GA3Each processing chlorophyll a, chlorophyll b, total chlorophyll content and carotenoid content with CK1 without significance difference
It is different.Work as GA3When concentration is 2.5 μm of ol/L, Cd stress lower romaine lettuce blade chlorophyll a, chlorophyll b and carotenoid content highest,
It is higher than CK1 by 9.80%, 11.48% and 3.19% respectively, it is not notable with CK differences.Spray GA3It is raw when concentration is 10 μm of ol/L
Dish leaf piece chlorophyll a, chlorophyll b and carotenoid content are minimum, lower than CK1 by 4.29%, 4.37% and 7.45% respectively,
With CK significant differences.
Table 3GA3The influence of lower romaine lettuce Contents of Photosynthetic Pigments is coerced Cd
External source GA3On the lower photosynthetic influence of romaine lettuce of Cd stress
As shown in Table 4, CK1 is compared with CK, and romaine lettuce Pn and Ls are significantly reduced, and Gs, Ci and Tr are significantly raised.Cd is coerced
Under, with GA3Concentration is raised, and romaine lettuce Pn is first raised and reduced afterwards, in GA3Concentration is 2.5 μm of ol/L highests, is improved compared with CK1
21.60% (P ＜ 0.05).Spray GA3When concentration is 10 μm of ol/L, romaine lettuce Pn is minimum, is reduced respectively compared with CK and CK1
31.23% (P ＜ 0.05) and 12.49% (P ＜ 0.05).Different from Pn variation tendencies, romaine lettuce Gs is with GA3Concentration is raised and first dropped
Raised after low.In GA3Concentration is minimum when being 2.5 μm of ol/L, reduces 25.32% (P ＜ 0.05) compared with CK1, but with CK phases
Than without significant difference.Under Cd stress, compared with CK1, low concentration (0~2.5 μm of ol/L) GA is sprayed3Significantly reduce romaine lettuce
Ci, works as GA3When concentration is 2.5 μm of ol/L minimum, (P ＜ 0.05) lower than CK1 by 11.23%.Spray GA3Concentration is 5 μm of ol/L and 10
During μm ol/L, though romaine lettuce Ci is more slightly higher than CK1, and it is not notable with CK1 differences.Spray GA3When concentration is 2.5 μm of ol/L, romaine lettuce Tr
Without significant difference compared with CK.Work as GA3When concentration is 1 μm of ol/L, 5 μm of ol/L and 10 μm of ol/L, Tr differences do not show between each processing
Write, respectively (P ＜ 0.05) lower than CK1 by 9.24%, 10.33% (P ＜ 0.05) and 7.61% (P ＜ 0.05).Spray GA3Concentration is
When 1 μm of ol/L, 2.5 μm of ol/L and 5 μm of ol/L, 24.43%, 52.84% and has been respectively increased in the significant difference compared with CK1
12.50%.
Table 4GA3On the lower photosynthetic influence of romaine lettuce of Cd stress
External source GA3The influence of lower romaine lettuce osmotic adjustment and root activity is coerced Cd
Cd stress causes romaine lettuce MDA contents significantly to raise, and sprays the GA of low concentration3Inhibited after (1~2.5 μm of ol/L)
MDA is generated.Work as GA3When concentration is 5 μm of ol/L and 10 μm of ol/L, 6.31% (P ＜ have been respectively increased in MDA contents compared with CK1
0.05) with 12.72% (P ＜ 0.05).Under Cd stress, work as GA3When concentration is 2.5 μm of ol/L (P ＜ 0.05), romaine lettuce MDA contents
It is minimum with electrical conductivity, (P ＜ 0.05) lower than CK1 by 15.27% and 12.62% (P ＜ 0.05) respectively.Spray 1 μm of ol/L, 5 μm of ol/
L and 10 μm of ol/L GA3Each processing, romaine lettuce electrical conductivity is substantially lower than CK1,8.65%, 5.74% and reduced respectively
5.42%.Spray GA3Afterwards, romaine lettuce proline content is significantly reduced, but GA3The proline content difference of each concentration processing is not notable.
GA3The romaine lettuce root activity of processing is followed successively by 2.5 μm of CK ＞, 5 μm of ol/L ＞, 1 μm of ol/L ＞, 10 μm of ol/L ＞ ol/L from high to low
＞ CK1.
Table 5GA3The influence of lower romaine lettuce osmotic adjustment and root activity is coerced Cd
External source GA3The influence of lower romaine lettuce activities of antioxidant enzymes is coerced Cd
As can be seen from Table 6, under Cd stress, with GA3The rise of concentration, romaine lettuce POD activity is drastically raised after first reducing,
GA3Concentration is minimum when being 2.5 μm of ol/L, and 14.97% (P ＜ 0.05) is reduced compared with CK1.Compared with CK1, GA is sprayed3Romaine lettuce
SOD contents are significantly reduced, and work as GA3When concentration is 1 μm of ol/L, 2.5 μm of ol/L, 5 μm of ol/L and 10 μm of ol/L, reduce respectively
24.31%th, 27.22%, 15.22% and 11.04%.For CAT activity, 1 μm of ol/L, 2.5 μm of ol/L and 5 μm of ol/L are sprayed
GA3Without significant difference between each processing, (P ＞ 0.05) higher than CK1 by 4.02%, 9.24% (P ＜ 0.05) and 8.03% (P respectively
＜ 0.05).Work as GA3When concentration is 10 μm of ol/L, romaine lettuce CAT activity is minimum, and difference is not notable compared with CK1.
Table 6GA3The influence of lower romaine lettuce activities of antioxidant enzymes is coerced Cd
External source GA3The influence of lower lettuce quality is coerced Cd
Under Cd stress, romaine lettuce soluble sugar content is with GA3The rise of concentration and first raise reduces afterwards, maximum is appeared in
GA3When concentration is 2.5 μm of ol/L, compared with CK1,6.75% (P ＜ 0.05) is improved.Work as GA3Concentration is 1 μm of ol/L and 5 μ
During mol/L, romaine lettuce soluble sugar content is compared with CK1 without significant difference.Work as GA3When concentration is 10 μm of ol/L, romaine lettuce is soluble
Sugared content is substantially less than CK1, reduces 21.35%.The minimum processing of Nitrate Content in Lettuce is GA3When concentration is 1 μm of ol/L,
Compared with CK1,45.55% (P ＜ 0.05) is reduced, afterwards with GA3The rise of concentration, Nitrate Content in Lettuce persistently rises
It is high.Spray GA3Though concentration is 2.5 μm of ol/L processing nitrate content than spraying GA3Concentration is slightly lower for 1 μm of ol/L processing, but
Difference is not notable between the two.With nitrate content variation tendency on the contrary, in GA3When concentration is 1 μm of ol/L, romaine lettuce soluble protein
Content reaches maximum, compared with CK1, improves 31.42% (P ＜ 0.05).Spray GA3Concentration is 2.5 μm of ol/L, 5 μm of ol/
Between L and 10 μm of ol/L each processing, without significant difference between soluble protein content.
Table 7GA3The influence of lower lettuce quality is coerced Cd
External source GA3Coerce Cd lower romaine lettuce Cd contents and Cd transports the influence of coefficient
Under Cd stress, romaine lettuce overground part Cd contents are with GA3Concentration is raised and slightly raised after reduction, works as GA3Concentration is 5 μ
75.56% (P ＜ 0.05) minimum during mol/L, being CK1.Spray GA3When concentration is 10 μm of ol/L, romaine lettuce overground part Cd contents
With spraying GA3Concentration is not notable for 2.5 μm of ol/L processing difference, but is substantially less than CK1.Spray low concentration (0~2.5 μ
mol/L)GA3Romaine lettuce root system Cd contents are not made significant difference, during high concentration (2.5~10 μm of ol/L), romaine lettuce root system Cd contents show
Write rise.Spray 5 μm of ol/L and 10 μm of ol/LGA3, romaine lettuce root system Cd contents are respectively 166.16mg/kg and 162.17mg/kg,
It is higher than CK1 by 12.65% and 10.31% respectively, with CK1 significant differences.When spraying GA3When concentration is 5 μm of ol/L, romaine lettuce Cd turns
Transport coefficient minimum, it is lower by 33.05% than compareing.
Table 8GA3Coerce Cd lower romaine lettuce Cd contents and Cd transports the influence of coefficient
GA3Handle the correlation analysis between Growth of Lettuce, physical signs and Cd contents
As can be seen from Table 9, GA is sprayed3Afterwards, the lower romaine lettuce overground part Cd contents of Cd stress and overground part fresh weight, root system are lived
Power, soluble sugar content and CAT activity, in extremely significantly negatively correlated, are in extremely notable positive correlation with POD and SOD activity.Under Cd stress,
Romaine lettuce overground part fresh weight and MDA contents are extremely significantly negatively correlated with POD, SOD activity in notable negatively correlated, with root activity, solvable
Property sugar and CAT activity be proportionate, and correlation is extremely notable.
Correlation analysis between the Growth of Lettuce of table 9, physical signs and Cd contents
Cd Pollution in Soil meeting coup injury root system of plant cell, suppresses absorption of the root system to nutrient, causes plant growth to delay
Slowly, blade flavescence is downright bad, plant is short and small, yield declines.Study influences of the various concentrations Cd to seedling growth of rice, find with
The increase of Cd concentration, seedling growth of rice is suppressed, and biomass is reduced.Also there are some researches show with the increase of Cd concentration, mustard
Dish is slow-growing, Aboveground Biomass of Young and relative water content are reduced].This experimental study is found, causes life under Cd stress
Dish plant height, stem is thick and biomass is significantly reduced.Meanwhile, Cd stress significantly reduces romaine lettuce root length and root activity.Under Cd stress,
Nitrate Content in Lettuce and soluble protein content are significantly raised, and nitrate content and overground part Cd contents are in notable positive
Close, illustrate that Cd concentration over-standards in soil are easily caused the accumulation of vegetable nitrate.
Plant is under normal physiological condition, and activity in vivo oxygen metabolism level is in relatively steady state.Coerced when by Cd
When compeling, cell membrane is destroyed, and causes active oxygen to be accumulated, and electrolyte largely exosmoses.Research shows that Cd also causes plant cell
Response to oxidative stress because Cd can effective Triggered Activity oxygen cluster synthesis and accumulation, cause cellular damage and Lipid peroxidation metabolism, press down
System or the activity for promoting antioxidase in antioxidant system.Gao Jiahe etc. thinks that Cd stress can improve flue-cured tobacco to a certain extent
Piece SOD and POD activity, but it is not avoided that the generation of cell membrane lipid peroxidating.This result of the test shows that Cd stress causes life
Dish SOD and POD activity rise, but CAT activity reductions.Show that romaine lettuce SOD and POD is stronger to Cd tolerance, in high concentrations of Cd
Under stress, both enzymatic activitys are still significantly raised, and CAT activity is then suppressed significantly.Under Cd stress, romaine lettuce electrical conductivity, dried meat
Histidine content and MDA contents are significantly raised, and show that high Cd stress romaine lettuce cell membrane is caused Lipid peroxidation metabolism, electrolyte by destruction
Extravasation.
Cd is a kind of photosynthetic effective inhibitor.Cd can be by reducing the content of chlorophyll, suppressing Calvin cycle
Reactions steps and the activity of enzyme reduce Pn, Gs, change the ultra microstructure of chloroplaset, by the generation for disturbing protochlorophyllide
To suppress the formation of chlorophyll, suppress the approach influence photosynthesis such as electron transport chain enzymatic activity related to photosynthetic carbon metabolism.
In this experiment, Cd stress causes romaine lettuce chlorophyll content to significantly reduce, and Pn is remarkably decreased, and Ci is raised, and shows the photosynthetic speed of romaine lettuce
Rate reduction is that Cd, which is coerced, causes photosynthetic related enzyme activity reduction and photosynthetic organs damage caused by Nonsto-matal factor.
Under heavy metal stress, plant inner chlorophyll acid esters reductase is suppressed, and intercellular membrane is destroyed, so that
Absorption and the activity of relevant enzyme of the photosynthesis, mineral element of plant are have impact on, causes plant cell division and elongation to be pressed down
System, biomass is substantially reduced.
This result of study shows, the GA of foliage-spray low concentration3Romaine lettuce biomass and plant height on Cd contaminated soils are improved,
And with GA3The increase of application concentration, romaine lettuce biomass and plant height are in the trend for first increasing and reducing afterwards, and especially plant height performance is the brightest
It is aobvious, spray GA3Concentration is significantly higher than unscared CK for 2.5~10 μm of ol/L processing plant height.Spray GA3Afterwards, romaine lettuce Pn liters
Height, and Ci and Tr reductions, show that GA3 alleviates destructions of the Cd to romaine lettuce photosynthetical system, while reducing the transpiration of romaine lettuce.
This result of study shows, suitable concentration GA3Processing makes romaine lettuce blade POD and SOD activity and the reduction of MDA contents, table
Bright GA3Effectively alleviate cell membrane to be hurt degree, reduce the oxidative stress of romaine lettuce, and then reduce POD and SOD are lured
Lead.Destruction of the active oxygen to chloroplaset is alleviated simultaneously, delays the aging of plant to a certain extent, C N metabolism is improved, from
And growth and the yield of romaine lettuce are promoted, but work as GA3When concentration is higher, this facilitation will weaken rapidly.
At present on GA3The report for adjusting plant absorption heavy metal is still rare.In the present invention, the GA of low concentration3Inhibit
Cd is transported to overground part, and romaine lettuce overground part Cd contents and transhipment coefficient are reduced, but work as GA3After more than 5 μm ol/L of concentration, romaine lettuce
Overground part Cd contents and transhipment coefficient rise, show the GA of suitable concentration3Cd can be suppressed to transport from root system to aerial part, can
Energy reason is GA3Transport of the ion on cell membrane can be influenceed by improving atpase activity, promote plant photosynthetic rate, reduction
Transpiration rate, so as to reduce the Cd contents of aerial part.
Comparative example 1
Except abscisic acid is changed into CKT, and concentration is that remaining is consistent with embodiment 1 outside 1~200 μm of ol/L.
Find after testing, in above-mentioned concentration range, the cadmium content of glass romaine lettuce aerial part, for CK1, most
Height only declines 1.2%.
Comparative example 2
Except abscisic acid is changed into JA, and concentration is that remaining is consistent with embodiment 1 outside 1~200 μm of ol/L.
Find after testing, in above-mentioned concentration range, the cadmium content of glass romaine lettuce aerial part, for CK1, most
Height only declines 1.6%.
Comparative example 3
Except abscisic acid is changed into EBR, and concentration is that remaining is consistent with embodiment 1 outside 1~200 μm of ol/L.
Find after testing, in above-mentioned concentration range, the cadmium content of glass romaine lettuce aerial part, for CK1, most
Height only declines 2.3%.
Comparative example 4
Except abscisic acid is changed into proline, and concentration is that remaining is consistent with embodiment 1 outside 1~200 μm of ol/L.
Find after testing, in above-mentioned concentration range, the cadmium content of glass romaine lettuce aerial part, for CK1, most
Height only declines 1.4%.
Comparative example 5
Except abscisic acid is changed into abscisic acid (ABA), and concentration is outside 1~20 μm of ol/L, remaining and embodiment 1 one
Cause.
Such as table 10, using abscisic acid, it is impossible to alleviate growth inhibition of the cadmium to romaine lettuce.
Table 10ABA coerces Cd the influence of lower Growth of Lettuce
Claims (6)
1. one kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content, it is characterised in that methods described comprises the following steps：
After Leaf lettuce seedling is ripe, with the GA that concentration is 1~10 μm of ol/L3The aqueous solution carries out foliage-spray, and the amount of spraying is with leaf
Face is dripped to spend, and was sprayed 1 time, is sprayed altogether 3 times every 2 days.
2. according to the method described in claim 1, it is characterised in that the GA3The concentration of the aqueous solution is 2.5~5 μm of ol/L.
3. method according to claim 1 or 2, it is characterised in that the GA3The concentration of the aqueous solution is 2.5 μm of ol/L.
4. according to the method described in claim 1, it is characterised in that utilizing GA3When the aqueous solution carries out foliage-spray, when spraying
Between be early morning.
5. according to the method described in claim 1, it is characterised in that spray GA the 1st time3The time of the aqueous solution is Leaf lettuce children
The 3rd day after seedling is ripe.
6. according to the method described in claim 1, it is characterised in that Leaf lettuce used is glass romaine lettuce.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710474319.1A CN107079707A (en) | 2017-06-21 | 2017-06-21 | One kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710474319.1A CN107079707A (en) | 2017-06-21 | 2017-06-21 | One kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107079707A true CN107079707A (en) | 2017-08-22 |
Family
ID=59606167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710474319.1A Pending CN107079707A (en) | 2017-06-21 | 2017-06-21 | One kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107079707A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651688A (en) * | 2019-11-07 | 2020-01-07 | 湖南省农业环境生态研究所 | Seedling culture substrate for culturing sedum plumbizincicola with low cadmium content and seedling culture method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102250949A (en) * | 2011-07-12 | 2011-11-23 | 中国计量学院 | Application of rice miR166 in enhancing plant cadmium stress tolerance |
| CN102553900A (en) * | 2010-12-29 | 2012-07-11 | 中国科学院沈阳应用生态研究所 | Method for enhanced remediation of cadmium-benzo[a]pyrene composite contaminated soil by combining gibberellin and Tween 80 |
-
2017
- 2017-06-21 CN CN201710474319.1A patent/CN107079707A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102553900A (en) * | 2010-12-29 | 2012-07-11 | 中国科学院沈阳应用生态研究所 | Method for enhanced remediation of cadmium-benzo[a]pyrene composite contaminated soil by combining gibberellin and Tween 80 |
| CN102250949A (en) * | 2011-07-12 | 2011-11-23 | 中国计量学院 | Application of rice miR166 in enhancing plant cadmium stress tolerance |
Non-Patent Citations (3)
| Title |
|---|
| ASIM MASOOD;M. IQBAL R. KHAN;MEHAR FATMA;MOHD ASGHER;TASIR S. PE: "Involvement of ethylene in gibberellic acid-induced sulfur ssimilation, photosynthetic responses, and alleviation of cadmium stress in mustard", 《PLANT PHYSIOLOGY AND BIOCHEMISTRY》 * |
| 张慧: "外源脱落酸(ABA)增强菹草抗镉(Cd2+)胁迫能力", 《生态与农村环境学报》 * |
| 邵君伟: "茉莉酸和赤霉素在水稻幼苗响应镉毒害中的调控作用", 《华中农业大学硕士学位论文》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110651688A (en) * | 2019-11-07 | 2020-01-07 | 湖南省农业环境生态研究所 | Seedling culture substrate for culturing sedum plumbizincicola with low cadmium content and seedling culture method thereof |
| CN110651688B (en) * | 2019-11-07 | 2022-04-08 | 湖南省农业环境生态研究所 | Seedling culture substrate for culturing sedum plumbizincicola with low cadmium content and seedling culture method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Maghsoudi et al. | Influence of foliar application of silicon on chlorophyll fluorescence, photosynthetic pigments, and growth in water-stressed wheat cultivars differing in drought tolerance | |
| Yang et al. | The impact of free-air CO2 enrichment (FACE) and N supply on yield formation of rice crops with large panicle | |
| Sali et al. | The effect of salt stress on the germination of maize (Zea mays L.) seeds and photosynthetic pigments | |
| You-Qiang et al. | Silicon-mediated amelioration of Fe2+ toxicity in rice (Oryza sativa L.) roots | |
| CN104335734B (en) | The method that the injury of Semen Maydis difference environment stress is alleviated in brassinosteroid seed soaking | |
| Zhao et al. | Effects of saline-alkaline stress on seed germination and seedling growth of Sorghum bicolor (L.) Moench | |
| Weng et al. | Mechanism of iodine uptake by cabbage: effects of iodine species and where it is stored | |
| CN107409800A (en) | Leaf lettuce cadmium content and the method for improving its quality are significantly reduced using salicylic acid | |
| CN108401872A (en) | It is a kind of to alleviate ciltivating process and salicylic application of the cadmium to tomato toxic action using salicylic acid | |
| Stefi et al. | The effect of the non ionizing radiation on cultivated plants of Arabidopsis thaliana (Col.) | |
| Liu et al. | Effects of different types of polyamine on growth, physiological and biochemical nature of lettuce under drought stress | |
| CN107251770A (en) | A kind of method for significantly reducing Leaf lettuce cadmium content | |
| Liao et al. | Antioxidant enzyme activity and growth responses of Huangguogan citrus cultivar to nitrogen supplementation | |
| Sysoeva et al. | Control of continuous irradiation injury on tomato plants with a temperature drop | |
| Sohail et al. | Effect of NaCl Salinity on Growth and Mineral Composition of Ziziphus spina-christi (L.) Willd. | |
| CN109197459A (en) | A method of alleviating sweet potato drought stress | |
| Tan et al. | Effects of waterlogging and cadmium on ecophysiological responses and metal bio-accumulation in Bermuda grass (Cynodon dactylon) | |
| CN107079707A (en) | One kind utilizes GA3The method for significantly reducing Leaf lettuce cadmium content | |
| CN107409799A (en) | Alleviate Leaf lettuce Cd stress growth inhibition and the method for reducing cadmium content using Mel | |
| CN107258356A (en) | Alleviate Leaf lettuce Cd stress growth inhibition and the method for reducing cadmium content using IAA | |
| CN107321774B (en) | Method for improving repair capacity of chickweed and bovine chickweed on cadmium-polluted soil by using mixed planting | |
| Muchkina et al. | The effect of nanoparticles of biogenic ferrihydrite on the development of Lepidium sativum L. | |
| CN106804153B (en) | Application method of different exogenous regulating substances to corn seed germination and seedling alleviation under low-temperature stress | |
| CN111171838B (en) | Exogenous additive for reducing absorption of tomato Cd, cultivation method and application | |
| CN107363081B (en) | Method for remarkably promoting cadmium-enriched plants to restore cadmium-contaminated soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170822 |