CN107063814A - Lead citrate staining solution and its compound method and application - Google Patents
Lead citrate staining solution and its compound method and application Download PDFInfo
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- CN107063814A CN107063814A CN201710374869.6A CN201710374869A CN107063814A CN 107063814 A CN107063814 A CN 107063814A CN 201710374869 A CN201710374869 A CN 201710374869A CN 107063814 A CN107063814 A CN 107063814A
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- lead citrate
- staining solution
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- lead
- citrate staining
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- HOQPTLCRWVZIQZ-UHFFFAOYSA-H bis[[2-(5-hydroxy-4,7-dioxo-1,3,2$l^{2}-dioxaplumbepan-5-yl)acetyl]oxy]lead Chemical compound [Pb+2].[Pb+2].[Pb+2].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HOQPTLCRWVZIQZ-UHFFFAOYSA-H 0.000 title claims abstract description 87
- 239000012192 staining solution Substances 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 16
- 150000001875 compounds Chemical class 0.000 title claims abstract description 15
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 70
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 52
- 239000002904 solvent Substances 0.000 claims abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000004043 dyeing Methods 0.000 claims description 37
- 230000010355 oscillation Effects 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 239000000203 mixture Substances 0.000 claims description 6
- 235000005979 Citrus limon Nutrition 0.000 claims description 5
- 244000248349 Citrus limon Species 0.000 claims description 3
- 244000131522 Citrus pyriformis Species 0.000 claims description 2
- 230000035939 shock Effects 0.000 claims 2
- 230000014759 maintenance of location Effects 0.000 abstract description 5
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 14
- 229910021642 ultra pure water Inorganic materials 0.000 description 13
- 239000012498 ultrapure water Substances 0.000 description 13
- 238000002360 preparation method Methods 0.000 description 12
- 230000000052 comparative effect Effects 0.000 description 10
- 235000015165 citric acid Nutrition 0.000 description 8
- 239000003643 water by type Substances 0.000 description 7
- 238000011109 contamination Methods 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000004140 cleaning Methods 0.000 description 5
- 230000010165 autogamy Effects 0.000 description 4
- 150000001860 citric acid derivatives Chemical class 0.000 description 4
- ABUBSBSOTTXVPV-UHFFFAOYSA-H [U+6].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O Chemical compound [U+6].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O ABUBSBSOTTXVPV-UHFFFAOYSA-H 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003960 organic solvent Substances 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000005352 clarification Methods 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000004080 punching Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 229920002527 Glycogen Polymers 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 229910052770 Uranium Inorganic materials 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000009514 concussion Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000012137 double-staining Methods 0.000 description 1
- 238000010894 electron beam technology Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- RYZCLUQMCYZBJQ-UHFFFAOYSA-H lead(2+);dicarbonate;dihydroxide Chemical compound [OH-].[OH-].[Pb+2].[Pb+2].[Pb+2].[O-]C([O-])=O.[O-]C([O-])=O RYZCLUQMCYZBJQ-UHFFFAOYSA-H 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- JKQOBWVOAYFWKG-UHFFFAOYSA-N molybdenum trioxide Chemical compound O=[Mo](=O)=O JKQOBWVOAYFWKG-UHFFFAOYSA-N 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 238000009971 piece dyeing Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000013049 sediment Substances 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- DNYWZCXLKNTFFI-UHFFFAOYSA-N uranium Chemical compound [U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U][U] DNYWZCXLKNTFFI-UHFFFAOYSA-N 0.000 description 1
- 125000005289 uranyl group Chemical group 0.000 description 1
- 235000021419 vinegar Nutrition 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Abstract
The present invention relates to a kind of lead citrate staining solution and its compound method and application.The lead citrate staining solution includes solvent, and concentration in the solvent be 30~40g/L lead citrate and concentration be 0.5~0.8g/L sodium hydroxide；The solvent is the mixed liquor of alcohol and water, wherein, the volumetric concentration of the alcohol is 25~33%.The lead citrate staining solution compound method is simple, and Color is good, is conducive to the presentation of ultra microstructure, and stable storing, retention cycle is long, and it is low to pollute small, cost.
Description
Technical field
The present invention relates to dyeing liquor, more particularly to lead citrate staining solution and its compound method and application.
Background technology
For the ultra-thin section of biological sample, image contrast source sample for electron beam scattering power, and its
Scattering power is constituted depending on atom：Atomic number is higher, and electron density is higher, and scattered electron ability is stronger, shows black under Electronic Speculum
Color；Atomic number is lower, and electron density is lower, and scattered electron ability is weaker, and white is shown under Electronic Speculum.And biological sample it is main by
The element composition of the low atomic numbers such as C, H, O, N, P, S, the ultra-thin section of no dyeing, contrast is very weak, particularly for medical science
The section of diagnosis, contrast effect difference can cause structure unintelligible, have a strong impact on the diagnostic result of pathologist.
Conventional thin section colouring liquid includes uranium acetate dyeing liquor and lead citrate staining solution at present.Wherein, vinegar
Sour uranyl：Also referred to as uranium acetate, it is dyed with improving based on nucleic acid, protein and the contrast of connective fiber to film
Effect is poor.Lead citrate：Density is big, has extensive affinity interaction to various institutional frameworks, especially to improve cell membrane system
And the contrast of lipid material is preferably, coloration is had more to the glycogen that can not be dyed by osmic acid.Because uranium and lead have difference
Dyeing characteristic, so section at present is universal all to use double staining.After first being dyed with uranium acetate, then use lead citrate
Dyeing, is complementary to one another, so as to obtain preferably Color.
But, the preparation of current lead citrate staining solution is relatively difficult, and easily in storage and dyeing course with sky
Carbon dioxide in gas combines to form the section of ceruse particle contamination, so as to influence electron microscopic observation, and with dyeing time with
And the increase of number of sections can increase the possibility of pollution.The lead citrate staining solution step of laboratory autogamy is more complicated, and
It is difficult to make solution transparent in process for preparation, and dyeing liquor is unstable, and storage a period of time (after such as two weeks), easily goes out after preparation
Now precipitate and cause dyeing liquor to use.And although the lead citrate staining solution retention cycle of commercialization is long, compound method
Extremely complex, if desired for lucifuge, closed stirring is carried out, filter bulb filtering, nitrogen charging, molten envelope etc. is operated, and common laboratory is difficult to
Prepare.In addition, to there is Color unstable for the lead citrate staining solution of laboratory autogamy and commercialization, easily sink
Form sediment, the problems such as Color is undesirable.
In addition, in the logical knowledge of those skilled in the art, water is the prioritizing selection solvent for being acknowledged as preparing dyeing liquor,
Because it can be very good dissolving solute (lead citrate), also will not occur abnormal response with lead citrate, solvent is used as with water
Solution, generally think have compared with the more preferable stability of organic solvent, simultaneously because lead citrate dye liquor is to pH value
Require, be also easy to adjust pH value as solvent with water.No matter therefore the citric acid of laboratory autogamy at present or commercialization
Lead dyeing liquor, is as solvent using the aqueous solution.And the dyeing liquor surface tension being thus made is big, easily form small with section
Bubble and cause diffused pollution, and dyeing when easily ultra-thin section surface formed minute bubbles, it is not easy to cut with ultra-thin
Piece is combined well, influences the definition of ultra-thin section.It is ultra-thin to cut after the completion of dyeing and dyeing liquor permeability is weaker
Piece is not easy to rinse well, and meeting more or less remains lead citrate staining solution and polluting causes ultra-thin section dyeing quality
Difference, while rinsing needs the ultra-pure water used many.The pollution of above-mentioned lead citrate staining solution and Cost Problems, and cut to ultra-thin
The influence problem of tablet quality is ignored by those skilled in the art always.
The content of the invention
Based on this, it is necessary to provide a kind of compound method simply, Color is good, is conducive to ultra microstructure to present, storage
Stable, retention cycle is long, and pollutes the low lead citrate staining solution of small, cost.
A kind of lead citrate staining solution, including solvent, and the citric acid that concentration in the solvent is 30~40g/L
Lead and the sodium hydroxide that concentration is 0.5~0.8g/L；
The solvent is the mixed liquor of alcohol and water, wherein, the volumetric concentration of the alcohol is 25~33%.
In the prior art, it is to use the aqueous solution no matter the lead citrate staining solution of laboratory autogamy or commercialization
It is used as solvent.The lead citrate staining solution of the present invention, breakthrough trial adds alcohol in lead citrate staining solution and is used as solvent
To reduce the surface tension of dyeing liquor.Found simultaneously by studying：Concentration such as alcohol is excessive, it is difficult to the solution of clarification is made, and
The stability of lead citrate staining solution can be influenceed；Concentration such as alcohol is too small, and the work for reaching reduction dyeing liquor surface tension is difficult to again
With.
Finally, being engaged containing alcoholic solvent with lead citrate and sodium hydroxide with designated volume concentration of the invention, not only carries
The concentration of effective ingredient lead citrate, strengthens the Color of dyeing liquor, shortens dyeing time in high lead citrate staining solution,
And the stability of obtained lead citrate staining solution is can also ensure that in this case, retention cycle is long.Dyeing is reduced simultaneously
The surface tension of liquid, makes obtained ultra-thin section laminating close, improves the permeability of dyeing liquor, Color is good, and be easy to punching
Wash, save the consumption for rinsing ultra-pure water, it is to avoid dyeing liquor residual causes lead contamination.
In one of the embodiments, in the solvent, the volumetric concentration of the alcohol is 28~32%.
In one of the embodiments, the alcohol is methanol and/or ethanol.In the lead citrate staining solution of the present invention,
Using methanol and/or ethanol as the alcohol, more preferably stability is resulted in.
In one of the embodiments, the alcohol is ethanol.Be preferred to use ethanol, can ensure stability while,
Reduce the toxicity of lead citrate staining solution.
In one of the embodiments, in the solvent, the concentration of the lead citrate is 34~36g/L, the hydrogen
The concentration of sodium oxide molybdena is 0.6~0.7g/L.
The present invention also provides the compound method of described lead citrate staining solution, comprises the following steps：
(1) by the lead citrate, alcohol, and the water isometric with the alcohol is mixed, and using ultrasonic oscillation, must be premixed
Thing；
(2) sodium hydroxide and remaining water are added in the pre-composition, then carries out ultrasonic oscillation, institute is produced
State lead citrate staining solution.
The compound method of lead citrate staining solution of the present invention, it is simple to operate, it is easy to control, in a short time
Rapid preparing is completed.Wherein, the water isometric with alcohol is first used in step (1), the concentration of alcohol can be suitably diluted, make lemon
Lead plumbate can preferably dissolve.
In one of the embodiments, the ultrasonic oscillation frequency is 35~45KHz.Utilize the ultrasonic wave of specific frequency
Concussion, can shorten the preparation time of dyeing liquor, reduce CO2The bad influence caused to dyeing liquor.
In one of the embodiments, in step (1), the time of the ultrasonic oscillation is 2~8min.
In one of the embodiments, in step (2), the time of the ultrasonic oscillation is 5~10min.
The present invention also provides application of the described lead citrate staining solution in ultra-thin section.
Compared with prior art, the invention has the advantages that：
Lead citrate staining solution of the present invention, it is all the aqueous solution to have broken current lead citrate staining solution formula
Tradition limitation, developing usable alcohol as solvent can also stablize and Color more preferably lead citrate staining solution.It has
Advantages below：
1st, Color is good：Alcohol is added in dyeing liquor as solvent, and improves active ingredient citric acid in dyeing liquor
The content of lead, thus, it is possible to preferably penetrate into section, is more beneficial for being combined with institutional framework, and then produces obvious contrast, makes to cut
Apparent ultra microstructure can be presented in piece under Electronic Speculum；
2nd, stable storing, retention cycle is long；
3rd, surface tension is small, and minute bubbles will not be formed with section, and pollution is few；
4th, after the completion of dyeing, section is easily rinsed well, and 100 sections are once contaminated with this dyeing liquor, also only needs 300mL to surpass
Pure water, washing time can be rinsed well for about 1 minute, will not washed clean because of punching, and dyeing liquor remains and causes section to occur
Lead contamination, and the cost of manufacture of section can also be reduced；
5th, dyeing liquor consumption is few：Because dyeing liquor surface tension is small, it is easier to spread in a slice uniformly, in cutting for isodose
In piece dyeing, 30% dyeing liquor can be at least saved；
6th, compound method is simple, and completion can be prepared in the short time；
7th, dyeing is may be directly applied to, without the operation such as centrifugation.
Brief description of the drawings
Fig. 1 is the ultra-thin section electron microscope that the lead citrate staining solution prepared using embodiment 1 is made；
Fig. 2 is the ultra-thin section electron microscope that the lead citrate staining solution prepared using embodiment 2 is made；
Fig. 3 is the ultra-thin section electron microscope that the lead citrate staining solution prepared using embodiment 3 is made；
Fig. 4 is the ultra-thin section electron microscope that the lead citrate staining solution prepared using comparative example 3 is made；
Fig. 5 is the ultra-thin section electron microscope that the lead citrate staining solution prepared using comparative example 4 is made.
Embodiment
Further is made to the lead citrate staining solution and its compound method of the present invention and application below in conjunction with specific embodiment
Detailed description.
In the embodiment of the present invention, the water is ultra-pure water, is specially the one-level for Milli-Q Gradient water purification machines
Water is made after home-use distillation machine redistillation once.
Former, enclosure material and equipment are as shown in table 1：
Table 1
Embodiment 1
A kind of lead citrate staining solution of the present embodiment, including solvent 100mL, and it is dissolved in the 3.5g citric acids of the solvent
The sodium hydroxide of lead and 0.064g.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 30%, i.e. 30mL.
The preparation method of above-mentioned lead citrate staining solution, comprises the following steps：
3.5g lead citrates are taken, 30mL ethanol and 30mL ultra-pure waters is added, ultrasonic oscillation 5min, frequency are carried out after mixing
For 40KHz, pre-composition is obtained；
Appropriate ultra-pure water dissolving 0.064g sodium hydroxide is taken to be made 16mL 1N sodium hydroxide solutions, and by the hydroxide
Sodium solution is added into above-mentioned pre-composition, then is settled to 100mL with ultra-pure water, and 5~10min of ultrasonic oscillation is then carried out again to be made
Solid is completely dissolved, and solution is limpid, produces the lead citrate staining solution, is dispensed with 5 or 10 milliliters of syringes, and sealing refrigeration is protected
Deposit, you can.
Dyed using the lead citrate staining solution, without any lead contamination, ultra microstructure is clear, and contrast is good (Fig. 1).Clearly
When washing, 100 sections are using 300mL ultrapure waters 1min.
Embodiment 2
A kind of lead citrate staining solution of the present embodiment, including solvent 100mL, and it is dissolved in the 3g lead citrates of the solvent
With 0.064g sodium hydroxide.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 30%, i.e. 30mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, ultra microstructure is clear, contrast (Fig. 2), it is desirable to implemented
Thus the Color of example 1 can increase multicontaminated probability, it is necessary to the time of proper extension dyeing.During cleaning, 100 sections
Using 300mL ultrapure waters 1min.
Embodiment 3
A kind of lead citrate staining solution of the present embodiment, including solvent 100mL, and it is dissolved in the 4g lead citrates of the solvent
With 0.064g sodium hydroxide.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 30%, i.e. 30mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, ultra microstructure is clear, and contrast is good (Fig. 3).But due to lead citrate
Concentration is raised, and increases CO2The probability in connection for producing precipitation.During cleaning, 100 sections use 400mL ultrapure waters
1min30S.
Embodiment 4
A kind of lead citrate staining solution of the present embodiment, including solvent 100mL, and it is dissolved in the 3.5g citric acids of the solvent
The sodium hydroxide of lead and 0.064g.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 25%, i.e. 25mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, ultra microstructure definition is subjected to.During cleaning, 100 sections are adopted
With 400mL ultrapure waters 1min30S.
Comparative example 1
A kind of lead citrate staining solution of this comparative example, including solvent 100mL, and it is dissolved in the 3.5g citric acids of the solvent
The sodium hydroxide of lead and 0.064g.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 35%, i.e. 35mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, organic solvent concentration is higher, be unfavorable for dye liquor configuration, be easily configured
Failure, shows as hardly resulting in clarification, free of contamination dye liquor.
Comparative example 2
A kind of lead citrate staining solution of this comparative example, including solvent 100mL, and it is dissolved in the 3.5g citric acids of the solvent
The sodium hydroxide of lead and 0.064g.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 40%, i.e. 40mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, organic solvent concentration is too high, be unfavorable for dye liquor configuration, cause configuration
Failure, shows as being clarified, free of contamination dye liquor.
Comparative example 3
A kind of lead citrate staining solution of this comparative example, including solvent 100mL, and it is dissolved in the 3.5g citric acids of the solvent
The sodium hydroxide of lead and 0.064g.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 20%, i.e. 20mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, ultra microstructure can be recognized, but definition is not enough (Fig. 4), and with showing
There is the lead citrate staining solution contrast prepared by solvent of water, effect is related not obvious enough.During cleaning, 100 sections are used
500mL ultrapure waters 2min.
Comparative example 4
A kind of lead citrate staining solution of this comparative example, including solvent 100mL, and it is dissolved in the 2g lead citrates of the solvent
With 0.064g sodium hydroxide.
The solvent is the mixed liquor of ethanol and ultra-pure water, wherein, the volumetric concentration of ethanol is 30%, i.e. 30mL.
The preparation method similar embodiment 1 of above-mentioned lead citrate staining solution.
Dyed using the lead citrate staining solution, ultra microstructure can be recognized, but definition is not enough (Fig. 5).Due to lemon
Lemon lead plumbate concentration is relatively low, it is desirable to obtain clearly ultra microstructure, it is necessary to extends dyeing time and rise temperature, greatly increases dirt
The probability of dye.During cleaning, 100 sections are using 300mL ultrapure waters 1min.
It is as shown in table 2 that embodiment 1-4 lead citrate staining solution prepares successfully test stone.
Table 2
Each technical characteristic of embodiment described above can be combined arbitrarily, to make description succinct, not to above-mentioned reality
Apply all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, the scope of this specification record is all considered to be.
Embodiment described above only expresses the several embodiments of the present invention, and it describes more specific and detailed, but simultaneously
Can not therefore it be construed as limiting the scope of the patent.It should be pointed out that coming for one of ordinary skill in the art
Say, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to the protection of the present invention
Scope.Therefore, the protection domain of patent of the present invention should be determined by the appended claims.
Claims (10)
1. a kind of lead citrate staining solution, it is characterised in that including solvent, and concentration in the solvent is 30~40g/
L lead citrate and concentration is 0.5~0.8g/L sodium hydroxide；
The solvent is the mixed liquor of alcohol and water, wherein, the volumetric concentration of the alcohol is 25~33%.
2. lead citrate staining solution according to claim 1, it is characterised in that in the solvent, the volume of the alcohol is dense
Spend for 28~32%.
3. lead citrate staining solution according to claim 1, it is characterised in that the alcohol is methanol and/or ethanol.
4. lead citrate staining solution according to claim 3, it is characterised in that the alcohol is ethanol.
5. the lead citrate staining solution according to claim any one of 1-4, it is characterised in that described in the solvent
The concentration of lead citrate is 34~36g/L, and the concentration of the sodium hydroxide is 0.6~0.7g/L.
6. the compound method of the lead citrate staining solution described in claim any one of 1-5, it is characterised in that including following step
Suddenly：
(1) by the lead citrate, alcohol, and the water isometric with the alcohol is mixed, and using ultrasonic oscillation, obtains pre-composition；
(2) sodium hydroxide and remaining water are added in the pre-composition, then carries out ultrasonic oscillation, the lemon is produced
Lemon lead plumbate dyeing liquor.
7. the compound method of lead citrate staining solution according to claim 6, it is characterised in that the ultrasonic oscillation
Frequency is 35~45KHz.
8. the compound method of lead citrate staining solution according to claim 7, it is characterised in that in step (1), described super
The time of sound wave shock is 2~8min.
9. the compound method of lead citrate staining solution according to claim 7, it is characterised in that in step (2), described super
The time of sound wave shock is 5~10min.
10. application of the lead citrate staining solution described in claim any one of 1-5 in ultra-thin section.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN101907533A (en) * | 2010-06-25 | 2010-12-08 | 绍兴文理学院 | Preparation method of tribasic lead staining fluid for electron microscopy experiment |
| WO2013093889A2 (en) * | 2011-12-23 | 2013-06-27 | L'oreal | Makeup process |
| CN106068260A (en) * | 2014-03-11 | 2016-11-02 | 三井化学株式会社 | The manufacture method of optical material episulfide compound, the compositions containing episulfide and comprise the polymerizable composition for optical material of said composition |
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| CN101907533A (en) * | 2010-06-25 | 2010-12-08 | 绍兴文理学院 | Preparation method of tribasic lead staining fluid for electron microscopy experiment |
| WO2013093889A2 (en) * | 2011-12-23 | 2013-06-27 | L'oreal | Makeup process |
| CN106068260A (en) * | 2014-03-11 | 2016-11-02 | 三井化学株式会社 | The manufacture method of optical material episulfide compound, the compositions containing episulfide and comprise the polymerizable composition for optical material of said composition |
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