CN107059428A - Method of the biosynthesis pyrrole structure red nano colorant dispersing liquid to wool fabric direct staining - Google Patents

Method of the biosynthesis pyrrole structure red nano colorant dispersing liquid to wool fabric direct staining Download PDF

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Publication number
CN107059428A
CN107059428A CN201710436306.5A CN201710436306A CN107059428A CN 107059428 A CN107059428 A CN 107059428A CN 201710436306 A CN201710436306 A CN 201710436306A CN 107059428 A CN107059428 A CN 107059428A
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China
Prior art keywords
wool fabric
pyrrole structure
red nano
structure red
dispersing liquid
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CN201710436306.5A
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Chinese (zh)
Inventor
巩继贤
任燕飞
张健飞
李政
李秋瑾
李辉芹
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Tianjin Polytechnic University
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Tianjin Polytechnic University
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Priority to CN201710436306.5A priority Critical patent/CN107059428A/en
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    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P1/00—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed
    • D06P1/16—General processes of dyeing or printing textiles, or general processes of dyeing leather, furs, or solid macromolecular substances in any form, classified according to the dyes, pigments, or auxiliary substances employed using dispersed, e.g. acetate, dyestuffs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06L—DRY-CLEANING, WASHING OR BLEACHING FIBRES, FILAMENTS, THREADS, YARNS, FABRICS, FEATHERS OR MADE-UP FIBROUS GOODS; BLEACHING LEATHER OR FURS
    • D06L1/00—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods
    • D06L1/12—Dry-cleaning or washing fibres, filaments, threads, yarns, fabrics, feathers or made-up fibrous goods using aqueous solvents
    • D06L1/16—Multi-step processes
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06P—DYEING OR PRINTING TEXTILES; DYEING LEATHER, FURS OR SOLID MACROMOLECULAR SUBSTANCES IN ANY FORM
    • D06P3/00—Special processes of dyeing or printing textiles, or dyeing leather, furs, or solid macromolecular substances in any form, classified according to the material treated
    • D06P3/02—Material containing basic nitrogen
    • D06P3/04—Material containing basic nitrogen containing amide groups
    • D06P3/14—Wool
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/425—Serratia
    • C12R2001/43—Serratia marcescens

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  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Textile Engineering (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Coloring (AREA)

Abstract

The present invention relates to a kind of method of biosynthesis pyrrole structure red nano colorant dispersing liquid to wool fabric direct staining, including：(1) high yield serratia marcescens fermenting and producing pyrrole structure red nano colorant dispersing liquid is utilized, using the fermentation medium of improvement, 0~48h adds nonionic surfactant system, lucifuge culture after inoculation.Centrifugation removes thalline after fermentation ends, obtains pyrrole structure red nano pigment dispersion dye liquor.(2) using above-mentioned dye liquor to wool fabric dyeing, dye bath pH is 2.0~10.0, and bath raio is 1: 10~50, dyes 20~60min at 80~100 DEG C, is finally soaped, washed.The preparation of pyrrole structure red nano pigment dispersion dye liquor and dyeing course are without using organic solvent, environmental protection in the present invention.Wool fabric after dyeing has good color fastness, bright in colour, to the bacteriostasis rate of staphylococcus aureus up to more than 95%, has a extensive future.

Description

Biosynthesis pyrrole structure red nano colorant dispersing liquid is to wool fabric direct staining Method
Technical field
It is more particularly to a kind of to be received by the pyrrole structure red of biosynthesis the invention belongs to the dyeing and finishing manufacture field of textile Direct staining method of the rice colorant dispersing liquid to wool fabric.
Technical background
Textile industry is produced and using about 1,300,000 tons of dyestuffs, pigment and dyestuff formers every year, is worth about 23,000,000,000 dollars, Nearly all it is artificial synthesized dyestuff, is made up completely of non-renewable resources such as oil.The row of its production, processing and dyeing waste liquid Environment of being rivals in a contest causes sizable harm.With the deterioration of the energy and environmental problem, people to the recyclability of dye source and The requirement more and more higher of green environmental protection.Natural dye is renewable with raw material, with good Environmental compatibility and biodegradation Property and receive significant attention, as study hotspot.Wherein the production of microbial dye/pigment is not limited by season, weather and region System, with short production cycle, yield is big, and species is enriched, and what is be exploited has a high potential, while environment and the ecological balance are protected, The contradiction of shortage of resources is solved, the advantage with Sustainable Development and Utilization.Therefore, microorganisms pigments will be increasingly becoming natural dye The main source of material.
Pyrrole structure pigment is the important species in biosynthesis pigment, is the secondary metabolite of microorganism, can be by thin Bacterium, actinomyces and mould obtain.Wherein, with 3 pyrrole rings red pigments research it is more, the pigment be soluble in methanol, ethanol, Acetone, DMF, ethyl acetate, chloroform and benzene, are practically insoluble in water.The pigment is mainly distributed on intracellular, the extraction needs of pigment Carry out clasmatosis.Because this pigment is water-soluble very low, pigment, which is extracted, will use organic solvent.There is research to attempt to be produced with microorganism Dyeing of the 3 pyrroles's ring structure red pigments to wool fabric, acrylic fabric and dacron.When preparing dye liquor, it is necessary to plus Enter organic solvent to dissolve pigment, this not only causes the increase of dye liquor preparation process process, it is often more important that dyeing has been significantly greatly increased Cost.
This research realizes the pyrrole structure of nanometer particle size by transformation and the improvement of incubation to microbial strains The biological preparation of red pigments, and realize uniform point secreted by cell into nanometer pigment in zymotic fluid in zymotic fluid Dissipate, thus prepare the dye liquor formed by nanoparticulate dispersion, the method to wool fabric direct staining is built on this basis.
The content of the invention
It is an object of the invention to provide a kind of nano dispersion fluid of use biosynthesis pyrrole structure red pigments to wool The direct staining method of fabric.
Use biosynthesis pyrrole structure red nano colorant dispersing liquid of the present invention is to wool fabric direct staining Method, including：
(1) serratia marcescens is rule on plating medium, selection color most red single bacterium colony is chosen, access fermentation Cultivated in culture medium to exponential phase, pipette 5ml bacterium solutions into a diameter of 9cm culture dish, power is 15W ultraviolet lamp Pipe distance 30cm irradiates 5~20min, is kept in dark place after 8-24h, is transferred in fermentation medium and cultivates to exponential phase.So It is transferred to the absorbance cultivated in Selective agar medium and λ=540nm is surveyed after 24h again afterwards, is then transferred to 10% inoculum concentration The absorbance that λ=540nm is surveyed after 24h is cultivated in new Selective agar medium, then is forwarded in new Selective agar medium, until inhaling Luminosity is not further added by.Bacterium solution 0.1ml is taken to be coated with 10 flat boards respectively, selection color most red single bacterium colony is chosen, access fermentation training Support and cultivated in base to exponential phase, adding lithium chloride makes its concentration be 0.1~1.5%, is incubated 0.5~10min, takes bacterium solution 5ml, which is transferred in fermentation medium, to be cultivated to exponential phase, be then transferred to again in Selective agar medium cultivate 24h after survey λ= 540nm absorbance, then is transferred in new Selective agar medium to cultivate and surveys λ=540nm's after 24h with 10% inoculum concentration Absorbance, then be forwarded in new Selective agar medium, until absorbance is not further added by.Bacterium solution 0.1ml is taken to be coated with 10 respectively Flat board, selection color most red single bacterium colony is chosen, the high-yielding engineering bacterial strain for being used as transformation to complete.
(2) superior strain is accessed in seed culture fluid and cultivated, 30 DEG C of temperature, 120~200rpm of shaking speed, during culture Between 6~24h.
(3) carried out in the fermentation medium that seed culture fluid is accessed to the improvement containing nonionic surfactant system A Zymotic fluid is centrifuged into 10min at 10000rpm, 10 DEG C after fermented and cultured, 48~144h of culture, thalline is removed, obtains pyrrole structure Red nano colorant dispersing liquid.
(4) the pyrrole structure nanometer colorant dispersing liquid prepared step (1) adjusts pH to be 2.0~10.0, and bath raio is 1: 10~ 50, it is put into after wool fabric, is heated up with 1~4 DEG C/min speed, 20~60min is dyed at 80~100 DEG C, then 20~40 DEG C washing 5~10min, 100 DEG C of 5~20min that soap, it is last 20~40 DEG C washing 5~10min.
In seed culture fluid described in step (2), following composition is contained in 1L culture mediums：Peptone 10g, dusty yeast 5g, Sodium chloride 3g, potassium chloride 2g, pH 5.0~8.0.
In the fermentation medium of improvement described in step (3), following composition is contained in 1L culture mediums：Peptone 15g, the third three Alcohol 3g, magnesium sulfate 2g, sodium chloride 3g, potassium chloride 2g, pH 5.0~8.0.
Condition of culture described in step (3) is：25~28 DEG C of cultivation temperature, 150~250rpm of shaking speed, the time 48~ 144h, lucifuge culture.
The composition of surfactant system A described in step (3) is 0~40g/L Tween-80,0~40g/L sucrose The AEO of fatty acid ester and 0~40g/L.
Through dyeing of the present invention dye after wool fabric have good rub resistance, it is resistance to soap and perspiration resistance color jail Degree, bright in colour, dyed fabric has good anti-microbial property, to the bacteriostasis rate of staphylococcus aureus up to more than 95%, tool There is good application prospect.And the invention can be applied to other water-insoluble microorganisms pigments, pass through the method for microbial fermentation Nanometer colorant dispersing liquid is directly produced, and realizes the direct staining to wool fabric.
Advantages of the present invention and beneficial effect are：
(1) preparation method of pyrrole structure red nano pigment dispersion dye liquor of the invention is simple, is directly sent out by microorganism Ferment is made, and needs not move through the extraction, concentration and dye liquor process for preparation of pigment, does not more need the complexity of General Decentralized liquid to prepare Journey, the requirement to equipment is low, not only time saving but also saving raw material.
(2) colouring method of the invention is simple, easily operation, the extraction of pigment, the preparation of dye liquor and the equal nothing of dyeing course Organic solvent need to be used, you can realize that the pyrrole structure haematochrome of biosynthesis, to wool fabric dyeing, is expanded and optimized non- Application of the water-soluble vitamins pigment in textile dyeing field.
Embodiment
For biosynthesis of the present invention nanometer colorant dispersing liquid to the direct staining method of wool fabric, had using following manner Body is implemented.But embodiment not limited to this of the present invention.
Embodiment 1
The preparation of biosynthesis pyrrole structure red nano pigment dispersion dye liquor.
High yield Serratia bacteria strain is accessed in seed culture fluid and cultivated, 30 DEG C of temperature, shaking speed 160rpm, training Support time 15h.Then seed liquor being seeded into the fermentation medium of improvement according to 2.0%, (peptone 15g/L, glycerine 3g/L, tells Temperature -80 18g/L, magnesium sulfate 2g/L, sodium chloride 3g/L, potassium chloride 2g/L) middle progress fermented and cultured 84h, 28 DEG C of temperature, shaking table Rotating speed 200rpm.Then zymotic fluid is centrifuged into 10min at 10000rpm, 10 DEG C, removes thalline, obtain pyrrole structure red nano Pigment dispersion dye liquor.The D50 particle sizes for determining pigment granule in dye liquor disperse system are 152.6nm, and average grain diameter is 253.8nm。
Embodiment 2
Direct staining of the pyrrole structure red nano pigment dispersion dye liquor to wool fabric.
Using the pyrrole structure red nano pigment dispersion dye liquor of preparation to wool fabric dyeing, dye liquor pH is 8, and bath raio is 1: 30, dyeing temperature is 90 DEG C, dyeing time 30min, then 25 DEG C of washing 5min, 100 DEG C of 10min that soap (twice), finally 25 DEG C of washing 5min.
According to national standard GB/T 3920-2008《Textile color stability tests colour fastness to rubbing》；GB/T 3921-2008 《Textile color stability tests fastness to soaping》；GB/T 3922-2013《Textile color stability tests colour fastness to perspiration》Survey Determine every color fastness of dyeing wool fabric, the results are shown in Table 1.
The dyeing wool fabric color fastness of table 1
According to national standard GB/T 20944.3-2008《The evaluation third portion of antibacterial textile performance：Succusion》To dyeing sheep Wool fabric carries out anti-microbial property test.Bacterium solution applies flat board bacteriostasis rate result of calculation and shown after oscillating contact：To golden yellow grape Coccus bacteriostasis rate is 96.8%.

Claims (1)

1. a kind of biosynthesis pyrrole structure red nano colorant dispersing liquid exists to the method for wool fabric direct staining, its feature In：Dye liquor is pyrrole structure red nano colorant dispersing liquid prepared by microbial fermentation, and dyeing temperature is 80~100 DEG C, heating Speed is 1~4 DEG C/min, and dye bath ratio is 1: 10~50, and dyeing time is 20~60min, and dye bath pH is 2.0~10.0.So Wash 5~10min, 100 DEG C of 5~20min that soap, 5~10min of last 20~40 DEG C of washings for 20~40 DEG C afterwards.
CN201710436306.5A 2017-06-08 2017-06-08 Method of the biosynthesis pyrrole structure red nano colorant dispersing liquid to wool fabric direct staining Pending CN107059428A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112574588A (en) * 2020-12-29 2021-03-30 泉州师范学院(石狮)生态智能织物工程技术研究院 Microbial pyrrole red pigment dye liquor and method for dyeing textiles by using same as dye

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434757A (en) * 2016-09-12 2017-02-22 天津工业大学 Microbial synthesis preparation method and application of cotton red nano pigment dyeing liquor
CN106434758A (en) * 2016-09-12 2017-02-22 天津工业大学 Method for dyeing polyester fiber fabric based on biologically-prepared nanometer pigment dye liquor

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106434757A (en) * 2016-09-12 2017-02-22 天津工业大学 Microbial synthesis preparation method and application of cotton red nano pigment dyeing liquor
CN106434758A (en) * 2016-09-12 2017-02-22 天津工业大学 Method for dyeing polyester fiber fabric based on biologically-prepared nanometer pigment dye liquor

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
任燕飞等: "纺织品染色用微生物色素的研究进展", 《纺织学报》 *
杨苏声: "《细菌分类学》", 31 August 1997, 中国农业大学出版社 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112574588A (en) * 2020-12-29 2021-03-30 泉州师范学院(石狮)生态智能织物工程技术研究院 Microbial pyrrole red pigment dye liquor and method for dyeing textiles by using same as dye

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