CN104109691A - Preparation method and dyeing method of red pigment secreted by ginkgo leaf endophyte - Google Patents
Preparation method and dyeing method of red pigment secreted by ginkgo leaf endophyte Download PDFInfo
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- CN104109691A CN104109691A CN201410322565.1A CN201410322565A CN104109691A CN 104109691 A CN104109691 A CN 104109691A CN 201410322565 A CN201410322565 A CN 201410322565A CN 104109691 A CN104109691 A CN 104109691A
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- ginkgo leaf
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Abstract
The invention relates to the field of light chemical industry, and aims to provide a preparation method and dyeing method of a red pigment secreted by ginkgo leaf endophyte. The preparation method comprises the following steps: washing ginkgo leaf endophyte spores, adding normal saline to prepare a spore suspension, inoculating in a PDA (potato dextrose agar) culture medium, culturing, and carrying out vacuum filtration after the culture is finished, thereby obtaining the solution containing the red pigment secreted by the ginkgo leaf endophyte. The dyeing method comprises the following steps: taking the solution containing the red pigment secreted by ginkgo leaf endophyte, diluting with water to obtain a dye solution, impregnating the dyed substance in the dye liquor to perform dyeing treatment, taking out the dyed substance after the dyeing is finished, cleaning and drying. The obtained novel natural dye is safe and environment-friendly. The red pigment has the advantages of favorable water solubility and higher substantivity for protein fibers. The fungus pigment is free from the limits of region and environment, and has favorable reproducibility. The biological culture method greatly enhances the yield, and is easies for implementing industrialization.
Description
Technical field
The invention relates to light industry field, particularly a kind of preparation method and dyeing process thereof of haematochrome of Ginkgo Leaf endophyte secretion.
Background technology
Along with the enhancing of people's environmental consciousness and the concern to self health, natural dyestuff more and more comes into one's own.Natural dyestuff does not pollute because of its preparation process, and biodegradable good with consistency environment, source is abundant, and DYED FABRICS wear safety, tone nature, and some also has good pharmacological action the liking of human consumers that be subject to getting more and more.
Natural dyestuff is vegetable dye, and plant-growth is subject to season, weather, and the shortcoming such as the impact of the environmental factorss such as the place of production, easily causes insufficient raw material, and extraction process is complicated, cost is higher and reproducibility is poor, so be not widely used.
Now people utilize bionic method artificial culture the various plants pigments such as madder, Asian puccoon.Utilize the method for microorganism culturing to prepare fungi pigment, overcome the defect of animals and plants dyestuffs, be not subject to region, environmental limit, compare with vegetable dye, the favorable reproducibility of fungi pigment, biological culturing method is accelerated Growth of Cells speed greatly, make like this production of natural dyestuff no longer only rely on natural plant, output increases substantially, and more easily realizes industrialization, meets the demand of people to natural dyestuff.
Researchist finds that the pigment of the microorganisms such as some bacterium, fungi also can be used as the source of natural dyestuff.Such as Britain research personnel, studying macro fungis such as finding palmate lead fungi, the fine pore fungi of coarse wool can the dyeing for multiple textiles as natural dyestuff.Japan silk insect agrotechnique institute and the joint study of silk company, found to produce the microorganism of blue or green purple pigment and to make it for dyeing silk.China is monascorubin for microorganisms pigments most study, and monascorubin is the pigment of monascus ruber filament secretion, is forming the throughput of producing nearly hundred tons per year at present, but is being mainly used in the painted application of food.The people such as Cheng Wanli have studied the application of red colouring agent for food, also used as a Chinese medicine class pigment on real silk fabric, but DYED FABRICS soaping fastness is poor.
About the preparation of Ginkgo Leaf endophyte secretion haematochrome and the applied research in textile dyeing thereof, there are no report.
Summary of the invention
Main purpose of the present invention is to overcome deficiency of the prior art, and a kind of natural red dyestuff and dyeing process thereof that is applied to textile dyeing is provided, and for solving the problems of the technologies described above, solution of the present invention is:
The preparation method of the haematochrome of a kind of Ginkgo Leaf endophyte secretion is provided, specifically comprises the steps: that the endophyte of getting Ginkgo Leaf washes lower spore by stroke-physiological saline solution, in spore, add physiological saline, spore concentration is modulated to 10
4～10
5cFU/ml, then connects bacterium amount (being that 100ml nutrient solution meets bacterium 1ml) by the spore suspension mixing up with 1% and is inoculated in PDA substratum and cultivates, and in temperature, is under 30 ℃～35 ℃, the pH value condition that is 5～7, cultivates 7～14 days; After cultivation finishes, suction filtration is removed thalline, obtains the solution of the haematochrome that contains the secretion of Ginkgo Leaf endophyte, can be for the dyeing of wool fabric and modification real silk fabric.
The dyeing process that the haematochrome of a kind of Ginkgo Leaf endophyte secretion based on described is provided, for pollutant is dyeed, specifically comprises the steps:
(1) get the solution of the haematochrome that contains Ginkgo Leaf endophyte secretion, be diluted with water to haematochrome content and be 30%～100% dye liquor;
(2) with the bath raio of 1:50, pollutant is immersed in the dye liquor of step (1), and regulates Value in Dyeing Process between 3～6 with Glacial acetic acid, processings of dye: 25 ℃ are dyed, with the speed of 2 ℃/min, be warming up to 30 ℃～80 ℃, be incubated 30～60min;
(3) pollutant is taken out, first with the hot water of 40 ℃, clean twice, then clean after twice with cold water, finally, with 60 ℃ of oven dry, complete the dyeing to pollutant.
As further improvement, described pollutant comprises the real silk fabric after wool fabric, pre-modification.
As further improvement, the pre-modification of described real silk fabric refers to: real silk fabric is immersed in and contains 4～10g/L properties-correcting agent and 0.5～2g/L Na
2cO
3modification liquid in, with the bath raio condition of 1:50, modification 30～60min at 30 ℃～60 ℃ temperature, then takes out real silk fabric washing and dries, and completes the pre-modification of real silk fabric.
As further improvement, described properties-correcting agent adopts commercially available cation modifier A (Guangzhou Heng Hua Fine Chemical Co., Ltd) or cation modifier CY-210 (Guangzhou Chuangyue Chemical Co., Ltd.).
Compared with prior art, the invention has the beneficial effects as follows:
1, the present invention obtains a kind of haematochrome of Ginkgo Leaf endophyte secretion, belongs to a kind of new type natural dyestuff, without the processing of any chemical reagent, pure natural dyestuff, safety and environmental protection, and this haematochrome has good water-solublely, for protein fibre, has higher substantivity;
2, the present invention utilizes the method for microorganism culturing to prepare natural dyestuff, can effectively avoid the shortcoming of vegetable dye, be not subject to region, environmental limit, the favorable reproducibility of fungi pigment, biological culturing method is accelerated Growth of Cells speed greatly, output increases substantially, and more easily realizes industrialization, meets the demand of people to natural dyestuff.
3, dyeing process of the present invention is taked substantive dyeing to wool fabric, and real silk fabric is carried out to the method for the pre-modification poststaining of cation modifier, increase the positive polarity of real silk fabric, strengthen the bonding force between haematochrome molecule and fiber, and then improve dyeing K/S value and the soaping fastness of this haematochrome on real silk fabric.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail:
A preparation method for the haematochrome of Ginkgo Leaf endophyte secretion, specifically comprises the steps: that the endophyte of getting Ginkgo Leaf washes lower spore by stroke-physiological saline solution, in spore, adds physiological saline, and spore concentration is modulated to 10
4～10
5cFU/ml, then connects bacterium amount (being that 100ml nutrient solution meets bacterium 1ml) by the spore suspension mixing up with 1% and is inoculated in PDA substratum and cultivates, and in temperature, is under 30 ℃～35 ℃, the pH value condition that is 5～7, cultivates 7～14 days; After cultivation finishes, suction filtration is removed thalline, obtains the solution of the haematochrome that contains the secretion of Ginkgo Leaf endophyte, can be for the dyeing of wool fabric and modification real silk fabric.
The dyeing process of the haematochrome of a kind of Ginkgo Leaf endophyte secretion based on described, for pollutant is dyeed, pollutant comprises the real silk fabric after wool fabric, pre-modification, and the pre-modification of real silk fabric refers to: real silk fabric is immersed in and contains 4～10g/L properties-correcting agent and 0.5～2g/L Na
2cO
3modification liquid in, with the bath raio condition of 1:50, modification 30～60min at 30 ℃～60 ℃ temperature, then takes out real silk fabric washing and dries, and completes the pre-modification of real silk fabric.Concrete dyeing process comprises the steps:
(1) get the solution of the haematochrome that contains Ginkgo Leaf endophyte secretion, be diluted with water to haematochrome content and be 30%～100% dye liquor;
(2) with the bath raio of 1:50, pollutant is immersed in the dye liquor of step (1), and regulates Value in Dyeing Process between 3～6 with Glacial acetic acid, processings of dye: 25 ℃ are dyed, with the speed of 2 ℃/min, be warming up to 30 ℃～80 ℃, be incubated 30～60min;
(3) pollutant is taken out, first with the hot water of 40 ℃, clean twice, then clean after twice with cold water, finally, with 60 ℃ of oven dry, complete the dyeing to pollutant.
Wherein, described properties-correcting agent adopts commercially available cation modifier A (Guangzhou Heng Hua Fine Chemical Co., Ltd) or cation modifier CY-210 (Guangzhou Chuangyue Chemical Co., Ltd.).
The following examples can make this professional professional and technical personnel's comprehend the present invention, but do not limit the present invention in any way.
Embodiment 1
10 liters of PDA substratum, Ginkgo Leaf endophyte concentration is 10
4～10
5the spore suspension inoculum size 1% of CFU/ml is 100ml, and temperature is 35 ℃, pH=5, and static cultivation 7 days obtains the haematochrome aqueous solution that Ginkgo Leaf endophyte is secreted after suction filtration.
Embodiment 2
10 liters of PDA substratum, Ginkgo Leaf endophyte concentration is 10
4～10
5the spore suspension inoculum size 1% of CFU/ml is 100ml, and temperature is 32 ℃, pH=6, and static cultivation 14 days obtains the haematochrome aqueous solution that Ginkgo Leaf endophyte is secreted after suction filtration.
Embodiment 3
10 liters of PDA substratum, Ginkgo Leaf endophyte concentration is 10
4～10
5the spore suspension inoculum size 1% of CFU/ml is 100ml, and temperature is 30 ℃, pH=7, and static cultivation 10 days obtains the haematochrome aqueous solution that Ginkgo Leaf endophyte is secreted after suction filtration.
Embodiment 4
1) wool fabric is immersed in the water, makes it fully wetting, extract stand-by;
2) wool fabric is immersed in embodiment 1 gained haematochrome dye liquor and dye, dye strength is 100%, bath raio 1:50,80 ℃ of temperature, pH=4, dyeing time 30min;
3) take out 40 ℃ of hot water wash of fabric after dyeing twice, twice of cold wash 60 ℃ of oven dry;
4) wool fabric after dyeing is scarlet, and to record color feature value be L:35.23a:27.68b:9.88c:29.39h:19.65K/S:9.2743 to machine color measurement and color match instrument as calculated.
Embodiment 5
1) wool fabric is immersed in the water, makes it fully wetting, extract stand-by;
2) wool fabric is immersed in embodiment 1 gained haematochrome dye liquor and dye, dye strength is 50%, bath raio 1:50, temperature 70 C, pH=3, dyeing time 60min;
3) take out 40 ℃ of hot water wash of fabric after dyeing twice, twice of cold wash 60 ℃ of oven dry;
4) wool fabric after dyeing is scarlet, and to record color feature value be L:36.63a:25.18b:9.26c:26.83h:20.20K/S:7.6651 to machine color measurement and color match instrument as calculated.
Embodiment 6
1) real silk fabric is immersed in the water, makes it fully wetting, extract stand-by;
2) real silk fabric is adopted to cation modifier A modification, properties-correcting agent consumption is 10g/L, Na
2cO
31.5g/L, bath raio 1:50, processes 40min, naturally dries for 30 ℃;
3) modification real silk fabric immerses in embodiment 2 gained haematochrome dye liquors and dyes, and dye strength is 30%, bath raio 1:50,40 ℃ of temperature, pH=3, dyeing time 60min;
4) take out 40 ℃ of hot water wash of fabric after dyeing twice, twice of cold wash 60 ℃ of oven dry;
5) real silk fabric after dyeing is garnet, L:50.5a:37.62b:14.88c:40.46h:21.58K/S:4.3522.
Embodiment 7
1) real silk fabric is immersed in the water, makes it fully wetting, extract stand-by;
2) real silk fabric is adopted to cation modifier CY-210 modification, properties-correcting agent consumption is 8g/L, Na
2cO
30.5g/L, bath raio 1:50, processes 50min, naturally dries for 40 ℃;
3) modification real silk fabric immerses in embodiment 2 gained haematochrome dye liquors and dyes, and dye strength is 70%, bath raio 1:50, temperature 50 C, pH=4, dyeing time 50min;
4) take out 40 ℃ of hot water wash of fabric after dyeing twice, twice of cold wash 60 ℃ of oven dry;
5) real silk fabric after dyeing is garnet, L:45.61a:39.08b:15.87c:42.18h:22.10K/S:6.3093.
Embodiment 8
1) real silk fabric is immersed in the water, makes it fully wetting, extract stand-by;
2) real silk fabric is adopted to cation modifier A modification, properties-correcting agent consumption is 6g/L, Na
2cO
31g/L, bath raio 1:50, processes 30min, naturally dries for 50 ℃;
3) modification real silk fabric immerses in embodiment 3 gained haematochrome dye liquors and dyes, and dye strength is 100%, bath raio 1:50,30 ℃ of temperature, pH=5, dyeing time 30min;
4) take out 40 ℃ of hot water wash of fabric after dyeing twice, twice of cold wash 60 ℃ of oven dry;
5) real silk fabric after dyeing is garnet, L:46.26a:36.46b:14.03c:39.07h:21.05K/S:5.5446.
Embodiment 9
1) real silk fabric is immersed in the water, makes it fully wetting, extract stand-by;
2) real silk fabric is adopted to cation modifier CY-210 modification, properties-correcting agent consumption is 4g/L, Na
2cO
32g/L, bath raio 1:50, processes 60min, naturally dries for 60 ℃;
3) modification real silk fabric immerses in embodiment 3 gained haematochrome dye liquors and dyes, and dye strength is 100%, bath raio 1:50, temperature 60 C, pH=6, dyeing time 60min;
4) take out 40 ℃ of hot water wash of fabric after dyeing twice, twice of cold wash 60 ℃ of oven dry;
5) real silk fabric after dyeing is garnet, L:44.24a:35.10b:13.33c:37.55h:20.79K/S:6.0009.
In sum, a kind of new type natural dyestuff of the present invention, can realize the dyeing to wool and modification real silk fabric, and wool fabric soaping fastness can reach 3～4 grades; Through cation modifier modification, can improve the dyeing K/S value of real silk fabric, make soaping fastness reach 3 grades.Utilize Ginkgo Leaf endophyte secretion haematochrome to dye to fabric, there is good environmental protection character, realized protein fabric green ecological dyeing and printing process.
Finally, it should be noted that above what enumerate is only specific embodiments of the invention.Obviously, the invention is not restricted to above embodiment, can also have a lot of distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (5)
1. a preparation method for the haematochrome of Ginkgo Leaf endophyte secretion, is characterized in that, specifically comprises the steps: that the endophyte of getting Ginkgo Leaf washes lower spore by stroke-physiological saline solution, in spore, adds physiological saline, and spore concentration is modulated to 10
4～10
5cFU/ml, then connects bacterium amount (being that 100ml nutrient solution meets bacterium 1ml) by the spore suspension mixing up with 1% and is inoculated in PDA substratum and cultivates, and in temperature, is under 30 ℃～35 ℃, the pH value condition that is 5～7, cultivates 7～14 days; After cultivation finishes, suction filtration is removed thalline, obtains the solution of the haematochrome that contains the secretion of Ginkgo Leaf endophyte, can be for the dyeing of wool fabric and modification real silk fabric.
2. the dyeing process of the haematochrome based on a kind of Ginkgo Leaf endophyte secretion claimed in claim 1, for pollutant is dyeed, is characterized in that, specifically comprises the steps:
(1) get the solution of the haematochrome that contains Ginkgo Leaf endophyte secretion, be diluted with water to haematochrome content and be 30%～100% dye liquor;
(2) with the bath raio of 1:50, pollutant is immersed in the dye liquor of step (1), and regulates Value in Dyeing Process between 3～6 with Glacial acetic acid, processings of dye: 25 ℃ are dyed, with the speed of 2 ℃/min, be warming up to 30 ℃～80 ℃, be incubated 30～60min;
(3) pollutant is taken out, first with the hot water of 40 ℃, clean twice, then clean after twice with cold water, finally, with 60 ℃ of oven dry, complete the dyeing to pollutant.
3. dyeing process according to claim 2, is characterized in that, described pollutant comprises the real silk fabric after wool fabric, pre-modification.
4. dyeing process according to claim 3, is characterized in that, the pre-modification of described real silk fabric refers to: real silk fabric is immersed in and contains 4～10g/L properties-correcting agent and 0.5～2g/L Na
2cO
3modification liquid in, with the bath raio condition of 1:50, modification 30～60min at 30 ℃～60 ℃ temperature, then takes out real silk fabric washing and dries, and completes the pre-modification of real silk fabric.
5. dyeing process according to claim 4, is characterized in that, described properties-correcting agent adopts commercially available cation modifier A or cation modifier CY-210.
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Cited By (3)
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| CN109400527A (en) * | 2018-04-20 | 2019-03-01 | 中国科学院成都生物研究所 | A kind of red edible fungus pigment and preparation method thereof with anti-inflammatory activity |
| CN111235916A (en) * | 2020-03-23 | 2020-06-05 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on bluish black purple bacillus spore powder |
| CN111286999A (en) * | 2020-03-23 | 2020-06-16 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on metarhizium anisopliae spore powder |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109400527A (en) * | 2018-04-20 | 2019-03-01 | 中国科学院成都生物研究所 | A kind of red edible fungus pigment and preparation method thereof with anti-inflammatory activity |
| CN111235916A (en) * | 2020-03-23 | 2020-06-05 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on bluish black purple bacillus spore powder |
| CN111286999A (en) * | 2020-03-23 | 2020-06-16 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on metarhizium anisopliae spore powder |
| CN111286999B (en) * | 2020-03-23 | 2022-05-31 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on metarhizium anisopliae spore powder |
| CN111235916B (en) * | 2020-03-23 | 2022-06-24 | 江苏鑫缘丝绸科技有限公司 | Silk floss dyeing method based on bluish black purple bacillus spore powder |
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