CN107058187A - It is a kind of to move Sphingomonas and its application less - Google Patents

It is a kind of to move Sphingomonas and its application less Download PDF

Info

Publication number
CN107058187A
CN107058187A CN201710379551.7A CN201710379551A CN107058187A CN 107058187 A CN107058187 A CN 107058187A CN 201710379551 A CN201710379551 A CN 201710379551A CN 107058187 A CN107058187 A CN 107058187A
Authority
CN
China
Prior art keywords
sphingomonas
exocellular polysaccharide
fjat
less
bacterial strain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201710379551.7A
Other languages
Chinese (zh)
Inventor
郑梅霞
朱育菁
刘波
陈峥
陈梅春
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Agricultural Biological Resources of Fujian Academy of Agricultural Sciences
Original Assignee
Institute of Agricultural Biological Resources of Fujian Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Agricultural Biological Resources of Fujian Academy of Agricultural Sciences filed Critical Institute of Agricultural Biological Resources of Fujian Academy of Agricultural Sciences
Priority to CN201710379551.7A priority Critical patent/CN107058187A/en
Publication of CN107058187A publication Critical patent/CN107058187A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention separates and screened the bacterial strain for obtaining being capable of high-yield extracellular polysaccharide from the cumquat blade of Nanping City in Fujian province Shunchang County, the bacterial strain moves the method that Sphingomonas FJAT 10625 prepares exocellular polysaccharide using this less to move Sphingomonas FJAT 10625 (Sphingomonas paucimobilis) less, and disclosing.The advantage of the invention is that：A kind of new Sphingomonas FJAT 10625 (Sphingomonas paucimobilis) dynamic less is provided, and the yield for preparing exocellular polysaccharide using the bacterial strain is higher, so as to add the source that exocellular polysaccharide produces bacterial strain；And it is simple, easily operated using the present invention process that dynamic Sphingomonas FJAT 10625 (Sphingomonas paucimobilis) prepares exocellular polysaccharide less.

Description

It is a kind of to move Sphingomonas and its application less
【Technical field】
The present invention relates to a kind of microorganism and its application, and in particular to a kind of to move Sphingomonas less and its answer With.
【Background technology】
Polysaccharide is using monose as basic composition unit, by the polymer of certain way repeated arrangement.For food plus The polysaccharide of work is commonly called as colloid, i.e. exocellular polysaccharide.Exocellular polysaccharide can derive from animal, plant, microorganism, seaweeds exocellular polysaccharide Mainly include agar and carragheen and alginic acid and its derivative.Plant exocellular polysaccharide includes Arabic gum, cellulose gum And its derivative, konjaku flour etc..Microbial exopolysaccharides mainly include xanthans, gellan gum, carragheen etc., with adherence, The features such as stability, emulsibility and gelation, suffer from being widely applied in terms of food, field of medicaments, it is especially near Nian Lai, with the introducing of Protocols in Molecular Biology, polysaccharide is in terms of anticoagulation, anti-aging, anti-cancer and cancer-preventing, disease immune Function is also gradually revealed.By its purposes, exocellular polysaccharide can be classified as thickener and gelling agent again, can be used as food additive Plus agent, coagulating agent, antistaling agent etc..Reported can the microorganism Pseudomonas of extracellular polysaccharide have Sphingomonas (Sphingomonas), Agrobacterium (Agrobacterium), xanthomonas (Xanthomonas) etc..But existing micro- life The yield of produce exocellular polysaccharide is relatively low, is the key constraints of the extensive industrialization of exocellular polysaccharide, therefore practitioner needs Further research, bacterial strain is produced to obtain the higher new exocellular polysaccharide of yield.
【The content of the invention】
The technical problems to be solved by the invention are that offer is a kind of and move Sphingomonas less, and the bacterial strain is few dynamic Sphingomonas FJAT-10625 (Sphingomonas paucimobilis), was preserved on 01 14th, 2016 China Committee for Culture Collection of Microorganisms's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Institute of Microorganism, Academia Sinica, deposit number be CGMCC NO.11996, and disclose prepared using the bacterial strain it is extracellular many Sugar.
The present invention is to solve above-mentioned technical problem by the following technical programs：
The applicant separates from the cumquat blade of Nanping City in Fujian province Shunchang County and screens that obtain can be high extracellular more The bacterial strain of sugar, by carrying out physio-biochemical characteristics measure and aliphatic acid Sherlock-MIDI network analyses to the bacterial strain, finally It is identified as moving a bacterial strain of Sphingol single-cell less.
Further, exocellular polysaccharide is prepared using the bacterial strain, its preparation method is concretely comprised the following steps：
(1) activation for moving Sphingomonas FJAT-10625 less described in claim 1：It will be weighed with oese Profit requires that the Sphingomonas FJAT-10625 that moves less described in 1 is lined on NA flat boards, and in constant incubator 48h is cultivated, and cultivation temperature is 30 DEG C；
(2) seed liquor is prepared：The single bacterium for moving Sphingomonas FJAT-10625 less that step (1) is obtained Fall to be inoculated in seed culture medium, and be placed in constant temperature oscillation shaking table and cultivate 16h, 30 DEG C of temperature, rotating speed 170rpm min-1；
(3) zymotic fluid is prepared：The seed liquor that step (2) is obtained is transferred to by 2% inoculum concentration by percent by volume Fermented and cultured 72h in the fermentation medium of sterilization, 30 DEG C of temperature, the rpmmin of rotating speed 190-1, produce zymotic fluid；
(4) extraction of exocellular polysaccharide：Zymotic fluid obtained by step (3) is centrifuged, supernatant is collected, to supernatant Ice ethanol is added in liquid, and the volume ratio of ice ethanol and supernatant is 3：1, after standing overnight sedimentation exocellular polysaccharide at 4 DEG C； Then the solution after standing is centrifuged, collects sediment；Using absolute ethyl alcohol washing precipitate 3 times, then nitrogen Drying, finally crushes and produces the exocellular polysaccharide product.
Further, the component of the NA flat boards is：Beef extract 3gL-1, peptone 5gL-1, glucose 10gL-1、 Yeast extract 1gL-1, agar powder 17gL-1、pH 7.2。
Further, the component of the seed culture medium is：Sucrose 20gL-1, peptone 5gL-1, yeast extract 5gL-1、pH 7.0。
Further, the component of the fermentation medium is：Glucose 30gL-1, peptone 3gL-1, yeast extract 2g·L-1、KH2PO4 1g·L-1、MgSO4 0.1g·L-1, micro- salting liquid 1mLL-1、 pH 7.0；
Wherein, the component of micro- salting liquid is：MnCl2·4H2O 1.8g·L-1、FeSO4·7H2O 2.4 g·L-1、H3BO3 0.283g·L-1、CuCl2 0.0027g·L-1、CoCl2·6H2O 0.0074g·L-1。
The beneficial effects of the present invention are：
A kind of new Sphingomonas FJAT-10625 (Sphingomonas dynamic less are provided Paucimobilis Sphingomonas FJAT-10625), and using this is moved less and prepares exocellular polysaccharide, and prepares born of the same parents The yield of exo polysaccharides is higher, i.e., this move less Sphingomonas FJAT-10625 can high-yield extracellular polysaccharide, so as to increase Exocellular polysaccharide produces the source of bacterial strain；And Sphingomonas FJAT-10625 is moved using the present invention less The process that (Sphingomonas paucimobilis) prepares exocellular polysaccharide is simple, easily operated.
【Brief description of the drawings】
The invention will be further described in conjunction with the embodiments with reference to the accompanying drawings.
Fig. 1 is the fatty acid profile of bacterial strain FJAT-10625 in the present invention.
【Embodiment】
A kind of Sphingomonas that moves less of the present invention is less dynamic Sphingomonas FJAT-10625 (Sphingomonas paucimobilis) is to separate and screen to obtain energy from the cumquat blade of Nanping City in Fujian province Shunchang County The bacterial strain of enough high-yield extracellular polysaccharides, and disclose moved less using this Sphingomonas FJAT-10625 prepare it is extracellular many The method of sugar.
In order to clearly illustrate the present invention, the applicant gives following several specific embodiments.
The separation of embodiment 1, bacterial strain FJAT-10625：
(1) the cumquat blade 5g of Nanping City in Fujian province Shunchang County is weighed respectively, first rinses blade with running water well, is inhaled The water of dry blade surface, soaks 30s in 75% alcohol afterwards, then soaks 5min with 10% sodium hypochlorite, then use sterilized water Rinse blade 3 times；
(2) in order to examine the effect of surface sterilizing, the blade after being handled through step (1) is clipped with sterilized tweezers, and Blade surface is contacted 2min with NA flat boards, then NA flat boards are placed at 30 DEG C and cultivate 2d, if finding there is no bacterium on NA flat boards Drop out existing, it was demonstrated that blade surface sterilizing is thorough, can be used as subsequent operation, otherwise giving up to use.
(3) sterilized thoroughly blade after step of learning from else's experience (2) inspection, and under sterile working, blade is shredded to mortar simultaneously 45mL sterilized waters are added, homogenate is fully ground and is made 10-1Sample stoste, stands 30min；Sample stoste is subjected to ladder successively afterwards Degree dilution, is 10 from dilution factor-2, 10-3Dilution；
(4) take the dilution of 200 μ L steps (3) selection and be added separately on NA flat boards, be applied to flat board drying, experiment weight It is multiple 3 times；NA flat boards after coating are placed in the bacterium grown after 30 DEG C of constant incubator culture, culture 24h on observation NA flat boards The form fallen, picking surface compact, color are golden yellow single bacterium colony；And the single bacterium colony of institute's picking is inoculated in seed liquor culture Cultivated in base, at 30 DEG C and seed liquor is obtained after 16h；Gained seed liquor is accessed in fermentation medium and carried out in fermented and cultured, fermentation Cultivate 72 h, 30 DEG C of temperature；(at room temperature, rotating speed 6000rpmmin is centrifuged in fermentation gained zymotic fluid-1Centrifugation 10min), supernatant is collected, ice ethanol is added into supernatant, and the volume ratio of ice ethanol and supernatant is 3：1, after 4 Sedimentation exocellular polysaccharide is stood overnight at DEG C；Then (at room temperature, rotating speed 6000rpm is centrifuged in the solution after standing min-1Centrifuge 10min), collect sediment；Using absolute ethyl alcohol washing precipitate 3 times, then nitrogen is dried up, and is finally crushed and is produced Exocellular polysaccharide product, claims dry weight；Compare the exocellular polysaccharide product yield of different strains, filter out exocellular polysaccharide product yield highest Bacterial strain, and by the Strain Designation be bacterial strain FJAT-10625.
The identification of embodiment 2, bacterial strain FJAT-10625
(1) physio-biochemical characteristics are identified：
The measure of the bacterial strain FJAT-10625 progress physio-biochemical characteristics obtained will be screened, obtain the Main Morphology of the bacterial strain Learn feature as follows：Bacterium colony is smaller, circular, yellow, smooth surface and moistening, micro- grand, neat in edge, it is opaque, with migration.
(2) Sherlock-MIDI system identifications：The microorganism automatic identifying system produced using MIDI companies of the U.S. (Sherlock Microbial Identification System) is analyzed bacterial strain FJAT-10625 aliphatic acid, point Analysis result shows that the bacterial strain FJAT-10625 aliphatic acid and Sherlock-MIDI systems Plays move Sphingol single-cell less The similarity factor of the aliphatic acid of bacterial strain is up to 0.709；Bacterial strain FJAT-10625 aliphatic acid figure is as shown in Figure 1.
The qualification result of 2 kinds of methods of summary, then judge bacterial strain FJAT-10625 to move Sphingol single-cell less (Sphingomonas paucimobilis)。
Embodiment 3, using bacterial strain FJAT-10625 prepare exocellular polysaccharide
Bacterial strain FJAT-10625 activation：Described is moved into Sphingomonas FJAT- less with oese 10625 line on NA flat boards, and cultivate in constant incubator 48h, and cultivation temperature is 30 DEG C；
Prepare seed liquor：Bacterial strain FJAT-10625 is activated to the Sphingomonas FJAT- dynamic less obtained 10625 single bacterium colony is inoculated in seed culture medium, and is placed in constant temperature oscillation shaking table and is cultivated 16h, 30 DEG C of temperature, is turned Fast 170rpmmin-1, obtain seed liquor；
Prepare zymotic fluid：The seed liquor of gained is transferred to the hair of sterilization by the inoculum concentration that percent by volume is 2% Fermented and cultured 72h in ferment culture medium, 30 DEG C of temperature, rotating speed 190rpmmin-1, produce zymotic fluid；
The extraction of exocellular polysaccharide：(at room temperature, the rpmmin of rotating speed 6000 is centrifuged in gained zymotic fluid-1Centrifugation 10min), supernatant is collected, ice ethanol is added into supernatant, and the volume ratio of ice ethanol and supernatant is 3：1, after 4 Sedimentation exocellular polysaccharide is stood overnight at DEG C；Then (at room temperature, rotating speed 6000rpm is centrifuged in the solution after standing min-1Centrifuge 10min), collect sediment；Using absolute ethyl alcohol washing precipitate 3 times, then nitrogen is dried up, and is finally crushed and is produced The exocellular polysaccharide product.
Embodiment 4, exocellular polysaccharide produced by the present invention are determined
The content of neutral polysaccharide in exocellular polysaccharide product made from embodiment 3 is determined using phend-sulphuric acid：Example Exocellular polysaccharide product accurate formulation 2gL made from 3-1Exocellular polysaccharide solution, measure the exocellular polysaccharide that 0.4mL prepared molten Liquid, and 1mL is complemented to using ultra-pure water, it is subsequently added into the phenol solutions of 0.5mL 6% and the 3mL concentrated sulfuric acids, boiling water after vibration is mixed 20min is bathed, room temperature is subsequently cooled to, absorbance is determined most at 490nm wavelength, using glucose as control, the result of measure Such as table 1 below.
The content of neutral sugar in the exocellular polysaccharide product of table 1
As shown in Table 1, the content of neutral polysaccharide is up to 2.333 gL in exocellular polysaccharide product produced by the present invention-1, because This, indicating being capable of high-yield extracellular polysaccharide using bacterial strain FJAT-10625 of the present invention.
It should be noted that in the present invention, the component of NA flat boards is：Beef extract 3gL-1, peptone 5gL-1, Portugal Grape sugar 10gL-1, yeast extract 1gL-1, agar powder 17gL-1、pH 7.2；The component of seed culture medium is：Sucrose 20gL-1, peptone 5gL-1, yeast extract 5gL-1、pH 7.0；The component of fermentation medium is：Glucose 30gL-1, peptone 3g·L-1, yeast extract 2gL-1、KH2PO4 1g·L-1、MgSO4 0.1g·L-1, micro- salting liquid 1mLL-1、pH 7.0；The component of micro- salting liquid is in fermentation medium：MnCl2·4H2O 1.8g·L-1、FeSO4·7H2O 2.4g· L-1、H3BO3 0.283g·L-1、CuCl2 0.0027g·L-1、CoCl2·6H2O 0.0074g·L-1.And without specified otherwise In the case of, the percentage in the present invention is mass percent.
To sum up, the present invention provides a kind of new Sphingomonas FJAT-10625 (Sphingomonas dynamic less Paucimobilis Sphingomonas FJAT-10625), and using this is moved less and prepares exocellular polysaccharide, and is prepared The yield of exocellular polysaccharide is higher, i.e., this move less Sphingomonas FJAT-10625 can high-yield extracellular polysaccharide, so as to increase Exocellular polysaccharide has been added to produce the source of bacterial strain；And Sphingomonas FJAT-10625 is moved using the present invention less The process that (Sphingomonas paucimobilis) prepares exocellular polysaccharide is simple, easily operated.

Claims (5)

1. a kind of move Sphingomonas less, it is characterised in that：The bacterial strain is dynamic Sphingomonas FJAT- less 10625 (Sphingomonas paucimobilis), Chinese microorganism strain preservation management was preserved on 01 14th, 2016 Committee's common micro-organisms center, deposit number is CGMCC NO.11996.
2. a kind of preparation method of exocellular polysaccharide, it is characterised in that：The preparation method specifically includes following steps：
(1) activation for moving Sphingomonas FJAT-10625 less described in claim 1：Will by right with oese Ask the Sphingomonas FJAT-10625 that moves less described in 1 to line on NA flat boards, and cultivated in constant incubator 48h, and cultivation temperature is 30 DEG C；
(2) seed liquor is prepared：The single bacterium colony inoculation for moving Sphingomonas FJAT-10625 less that step (1) is obtained In seed culture medium, and it is placed in constant temperature oscillation shaking table and cultivates 16h, 30 DEG C of temperature, rotating speed 170rpmmin-1；
(3) zymotic fluid is prepared：The seed liquor that step (2) is obtained is transferred to by 2% inoculum concentration by percent by volume and sterilized Fermented and cultured 72h in the fermentation medium of sterilizing, 30 DEG C of temperature, rotating speed 190rpmmin-1, produce zymotic fluid；
(4) extraction of exocellular polysaccharide：Zymotic fluid obtained by step (3) is centrifuged, supernatant is collected, into supernatant Ice ethanol is added, and the volume ratio of ice ethanol and supernatant is 3：1, after standing overnight sedimentation exocellular polysaccharide at 4 DEG C；Then Solution after standing is centrifuged, sediment is collected；Using absolute ethyl alcohol washing precipitate 3 times, then nitrogen is dried up, Finally crush and produce the exocellular polysaccharide product.
3. a kind of preparation method of exocellular polysaccharide according to claim 2, it is characterised in that：The component of the NA flat boards For：Beef extract 3gL-1, peptone 5gL-1, glucose 10gL-1, yeast extract 1gL-1, agar powder 17gL-1、pH 7.2。
4. a kind of preparation method of exocellular polysaccharide according to claim 2, it is characterised in that：The group of the seed culture medium It is divided into：Sucrose 20gL-1, peptone 5gL-1, yeast extract 5gL-1、pH 7.0。
5. a kind of preparation method of exocellular polysaccharide according to claim 2, it is characterised in that：The group of the fermentation medium It is divided into：Glucose 30gL-1, peptone 3gL-1, yeast extract 2gL-1、KH2PO4 1g·L-1、MgSO4 0.1g·L-1, it is micro- Secondary element salting liquid 1mLL-1、pH 7.0；
Wherein, the component of micro- salting liquid is：MnCl2·4H2O 1.8g·L-1、FeSO4·7H2O 2.4g·L-1、H3BO3 0.283g·L-1、CuCl2 0.0027g·L-1、CoCl2·6H2O 0.0074g·L-1。
CN201710379551.7A 2017-05-25 2017-05-25 It is a kind of to move Sphingomonas and its application less Pending CN107058187A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710379551.7A CN107058187A (en) 2017-05-25 2017-05-25 It is a kind of to move Sphingomonas and its application less

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710379551.7A CN107058187A (en) 2017-05-25 2017-05-25 It is a kind of to move Sphingomonas and its application less

Publications (1)

Publication Number Publication Date
CN107058187A true CN107058187A (en) 2017-08-18

Family

ID=59609829

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710379551.7A Pending CN107058187A (en) 2017-05-25 2017-05-25 It is a kind of to move Sphingomonas and its application less

Country Status (1)

Country Link
CN (1) CN107058187A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113957030A (en) * 2021-11-05 2022-01-21 南开大学 Sphingomonas strain with characteristic of efficiently synthesizing Wzy type extracellular polysaccharide and construction method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1970738A (en) * 2006-09-12 2007-05-30 山东大学 Sphingomonaspaucimobilis of high-yield gellan gum and use therefor
CN102747016A (en) * 2012-06-28 2012-10-24 福建省农业科学院农业生物资源研究所 Gellan gum efficient production strain and its application
CN103421718A (en) * 2013-08-09 2013-12-04 浙江大学 Sphingomonas paucimobilis strain and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1970738A (en) * 2006-09-12 2007-05-30 山东大学 Sphingomonaspaucimobilis of high-yield gellan gum and use therefor
CN102747016A (en) * 2012-06-28 2012-10-24 福建省农业科学院农业生物资源研究所 Gellan gum efficient production strain and its application
CN103421718A (en) * 2013-08-09 2013-12-04 浙江大学 Sphingomonas paucimobilis strain and application thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113957030A (en) * 2021-11-05 2022-01-21 南开大学 Sphingomonas strain with characteristic of efficiently synthesizing Wzy type extracellular polysaccharide and construction method and application thereof
CN113957030B (en) * 2021-11-05 2024-01-05 南开大学 Sphingomonas strain with characteristic of synthesizing Wzy type extracellular polysaccharide, construction method and application thereof

Similar Documents

Publication Publication Date Title
CN103421718B (en) A kind of sphingomonas paucimobilis bacterial strain and application thereof
CN104328064A (en) Bacillus natto and application of Bacillus natto in fermentation production of vitamin K2
JP6265407B2 (en) Method for culturing squalene-producing algae using shochu wastewater as culture medium
CN102747016A (en) Gellan gum efficient production strain and its application
CN110218667A (en) One plant of bacterial strain SH-1 for producing alginate lyase and its application
CN113755544B (en) Schizophyllum commune fermentation product, and preparation method and application thereof
CN104611236B (en) Cunninghamella echinulata FAR3 and fermentation thereof are for the method for gamma-linolenic acid oil
CN107034164A (en) It is a kind of to move Sphingomonas and its application less
CN103589759B (en) A kind of waste molasses and the two carbon source through fermentation of sewer oil produce the method for sophorolipid
CN113750008B (en) Jasmine flower enzymolysis fermentation product and preparation method and application thereof
CN104651284B (en) Sphingol single-cell T 3 and its method for common fermentation production biological polyoses and poly- β hydroxybutyric acids
CN107058187A (en) It is a kind of to move Sphingomonas and its application less
CN105505816A (en) Sphingomonas paucimobilis strain and application thereof
CN103468583B (en) Penicillium oxalicum WX-209 strain with high cellulase yield and enzyme producing method
CN104164385A (en) Sphingomonas paucimobilis strain gxas-815 and applications thereof
CN104404016A (en) Naringinase production method
CN106755184B (en) Thelephora ganbajun mycelium polysaccharide and its preparation method and application
CN103898012A (en) Thalassospira sp. strain and method for preparing agarase
CN106636252A (en) Thelephora ganbajun Zang exopolysaccharide, preparation method thereof and application of exopolysaccharide
CN106119144A (en) A kind of Pericarppium arachidis hypogaeae hydrolysate that converts prepares method and the bacterial strain of microbial flocculant
CN110616150A (en) High-yield polysaccharide eupatorium adenophorum endophytic fungus and application thereof
CN101497902A (en) Process for preparing microbe oil
CN110760451B (en) Yarrowia lipolytica and application thereof in preparation of low-sugar low-fat desiccated coconut nutrition powder
CN100567479C (en) A kind of artificial culturing method of Sanguis Draxonis
CN104726371B (en) A kind of Xanthomonas axonopodis bacterial strain and application

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170818

RJ01 Rejection of invention patent application after publication