CN107047299A - A kind of potato stem section tissue culture medium (TCM) and its cultural method - Google Patents
A kind of potato stem section tissue culture medium (TCM) and its cultural method Download PDFInfo
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- CN107047299A CN107047299A CN201710145024.XA CN201710145024A CN107047299A CN 107047299 A CN107047299 A CN 107047299A CN 201710145024 A CN201710145024 A CN 201710145024A CN 107047299 A CN107047299 A CN 107047299A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Abstract
The present invention relates to field of plant tissue culture technique, and in particular to a kind of potato stem section tissue culture medium (TCM), in addition to potato test tube seedling stem section one-step culture method.Grown required inorganic nutritive element and part organic nutrition element from MS conventional mediums there is provided cultured tissue；The culture medium that the present invention is provided has broad spectrum activity, is adapted to majority Potato Cultivars stem section tissue cultures forming seedling through one step culture.The present invention is by rationally controlling hormone dosage and mutual proportioning, and the addition of other auxiliary materials, make stem section explant after callus is formed, it need not be transferred on differential medium, but the direct seedling differentiation on former culture medium, shorten incubation time two months or so, reduce financial cost；And the culture medium has broad spectrum activity, it is adapted to most of kinds.
Description
Technical field
The present invention relates to field of plant tissue culture technique, and in particular to a kind of potato stem section tissue culture medium (TCM), also wraps
Include potato test tube seedling stem section one-step culture method.
Background technology
Potato (Solanum Tuberosum L.) is Solanaceae Solanum annual herb tuberous plant, be important grain,
Dish, feed and the raw material of industry and crops, occupy considerable status in China's agricultural production.In order to which Innovation Germplasm is provided
Source, tissue culture technique is widely used in Potato Breeding research, and achieves notable achievement.
In recent years, the fast development of biotechnology brings vitality for the research and production of potato, passes through tissue
Culture and genetic transformation carry out potato genetic improvement, it has also become the importance of potato molecular breeding.However, either losing
The technologies such as the foundation of transformation system, or the screening of mutant, the quick breeding of good seed and somatic hybridization are passed all to depend on
Link is regenerated in tissue cultures.The foundation of regenerating system is the theoretical foundation for having totipotency using the plant cell of life, is led to
Cross callus regeneration and form new plant, i.e., by being transferred to explant progress callus induction, then by callus
Seedling differentiation obtains regeneration plant on differential medium.The explant used in current Regeneration System of Potato mainly has test tube
Blade, stem section, petiole, root of seedling etc., mainly using explant-callus (Fiber differentiation)-differentiation seedling (differentiation cultivate)-
Take root multiple culture programs such as (culture of rootage).Explant is subjected to callus tissue culture, callus is induced, then will be more
Injured tissue is transferred to regeneration plant in differential medium.Through multistep ways for training is loaded down with trivial details, the universal relatively low and induction time of regeneration rate
It is longer, and regenerative system is easily by genotype, explant species, the species of plant hormone and concentration, condition of culture and operation skill
The factors such as art level influence, and the regenerative system of Potato Varieties is there is larger difference.This patent is asked for these
Topic has researched and proposed the one-step culture base using stem section as explant, and the culture medium makes potato test tube seedling stem section tissue cultures
Forming seedling through one step culture, short with the regeneration period, breeding coefficient and regeneration frequency are high, and genotypic adaptation wide spectrum is easy to operate, cultivate program
Simple the advantages of, quickly bred for potato good seed, screening mutant and breed improvement provide an effective way.
The content of the invention
It is a primary object of the present invention to regeneration frequency present in being studied for Regeneration System of Potato is low, induction time
Long, there is provided a kind of potato stem section tissue of forming seedling through one step culture for the problems such as regeneration induction condition has differences between different genotype
Culture medium and its cultural method.Potato test tube seedling stem section is after callus is formed, it is not necessary to be transferred on differential medium,
But the direct seedling differentiation on former culture medium, improve the regeneration efficiency of potato test tube seedling callus from stem segment, shorten
Incubation time, simplify operating procedure, and genotypic adaptation wide spectrum, be potato good seed quickly breed, mutant sieve
Choosing and breed improvement provide an effective way.
The technical solution adopted for the present invention to solve the technical problems is:
A kind of potato stem section tissue culture medium (TCM) and its cultural method, comprise the following steps：
A. prepared by culture medium
1~3mg 6- benzylaminopurines, 0.01~0.1mg methyl α-naphthyl acetates, 2.5~7.5mg are added in every liter of MS culture medium red mould
Element, 50-100mg inositols, 25~30g edible sugars and 3~4.5g agar；
B. medium treatment
Culture medium is heated to being stirred continuously in boiling, heating process, and pH value is adjusted into 5.8~6.0 after agar is completely dissolved；
High pressure steam sterilization after packing, the culture medium after sterilizing stands cooling solidification；
C. seedling process is connect
Choose under the seedling age potato in vitro cuttings of 20~30 days, aseptic condition, cut stems of the 0.5cm~1cm without axillary bud
Section, is inoculated in step B culture medium；
D. seedling culture is connect
By step C be inoculated with stem section culture medium be placed in temperature for 21 ± 2 DEG C, 3500~4000LX of intensity of illumination, light application time
Cultivated under conditions of 16h/ days；
E. subsection filter
Culture 15 days, stem section is expanded, and has Callus formation, and surface forms particulate material or strumae；Culture 25~30
My god, callus from stem segment differentiation Multiple Buds；Culture 28-35 days, obtains potato regeneration plant.
Culture medium is heated to being stirred continuously in boiling, heating process in the step B, by pH value after agar is completely dissolved
It is adjusted to 5.8~6.0；Under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa sterilization 15~
20min, the culture medium after sterilizing stands cooling solidification.
3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg gibberellin, 100mg are added in every liter of MS culture mediums
Inositol.
Beneficial effects of the present invention are：
Inorganic nutrients needed for the minimal medium of the present invention is grown from MS conventional mediums there is provided cultured tissue are first
Element and part organic nutrition element；6- benayl aminopurines, methyl α-naphthyl acetate, gibberellin are plant growth regulator, with promotion training
Support the effect for organizing the formation of Multiple Buds；Inositol promotes callus growth and bud to be formed；Edible sugar provides cultured tissue growth
Carbon nutrition needed for development；The main function of agar is solidification culture medium, is played a supportive role.The culture medium tool that the present invention is provided
There is broad spectrum activity, be adapted to majority Potato Cultivars stem section tissue cultures forming seedling through one step culture.The present invention by rationally control hormone dosage and
Mutual proportioning, and other auxiliary materials addition, make stem section explant formed callus after, it is not necessary to be transferred to differentiation
On culture medium, but the seedling differentiation directly on former culture medium, shorten incubation time two months or so, reduce financial cost；
And the culture medium has broad spectrum activity, it is adapted to most of kinds.The culture medium makes potato test tube seedling stem section tissue cultures one
Seedling is walked, short with the regeneration period, breeding coefficient and regeneration frequency are high, and genotypic adaptation wide spectrum is easy to operate, culture program letter
Single the advantages of, quickly bred for potato good seed, screening mutant and breed improvement provide an effective way.
Brief description of the drawings
The Multiple Buds figure that Fig. 1 cultivates for the present invention；
The potato plant figure that Fig. 2 cultivates for the present invention；
The Potato regeneration figure that Fig. 3 cultivates for the present invention；
No. 12 regeneration figures of potato Gansu Province potato that Fig. 4 cultivates for the present invention；
No. 8 regeneration figures of potato Gansu Province potato that Fig. 5 cultivates for the present invention；
No. 7 regeneration figures of potato Gansu Province potato that Fig. 6 cultivates for the present invention；
No. 9 regeneration figures of potato Gansu Province potato that Fig. 7 cultivates for the present invention；
The potato LK99 regeneration figures that Fig. 8 cultivates for the present invention；
No. 11 regeneration figures of potato Gansu Province potato that Fig. 9 cultivates for the present invention；
No. 14 regeneration figures of potato Gansu Province potato that Figure 10 cultivates for the present invention；
No. 4 regeneration figures of potato Gansu Province potato that Figure 11 cultivates for the present invention；
No. 5 regeneration figures of potato Gansu Province potato that Figure 12 cultivates for the present invention；
The Longshu No.3 Potato Variety regeneration figure that Figure 13 cultivates for the present invention.
Embodiment
A kind of potato stem section tissue culture medium (TCM) and its cultural method, it is characterised in that comprise the following steps：
It is prepared by A, culture medium
1~3mg 6- benzylaminopurines, 0.01~0.1mg methyl α-naphthyl acetates, 2.5~7.5mg are added in every liter of MS culture medium red mould
Element, 50-100mg inositols, 25~30g edible sugars and 3~4.5g agar；
B, medium treatment
Culture medium is heated to being stirred continuously in boiling, heating process, and pH value is adjusted into 5.8~6.0 after agar is completely dissolved；
15~20min, the culture medium after sterilizing are sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa
Stand cooling solidification；
C, connect seedling process
Choose under the seedling age potato in vitro cuttings of 20~30 days, aseptic condition, cut stems of the 0.5cm~1cm without axillary bud
Section, is inoculated in step B culture medium；
D, connect seedling culture
By step C be inoculated with stem section culture medium be placed in temperature for 21 ± 2 DEG C, 3500~4000LX of intensity of illumination, light application time
Cultivated under conditions of 16h/ days；
E, subsection filter
Culture 15 days, stem section is expanded, and has Callus formation, and surface forms particulate material or strumae；Culture 25~30
My god, callus from stem segment differentiation Multiple Buds；Culture 28-35 days, obtains potato regeneration plant.
It is preferred that every liter of MS culture mediums in addition 3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg it is red mould
Element, 100mg inositols.
Following examples are elaborated for the present invention, and Fig. 3 to Figure 13 is respectively each step of kind test tube seedling stem section one regeneration
Seedling.
Test material selects the detoxification test tube plantlet of Potato Cultivars Gansu Province potato 8, and by academy of agricultural sciences of Gansu Province, potato is provided.
Using MS minimal mediums as control, using L16(45) orthogonal design, in MS culture mediums add various concentrations and
The 6- benzylaminopurines of combination（6-BA）, methyl α-naphthyl acetate（NAA）, gibberellin（GA3）And inositol, it is configured to 16 groups of different cultures
Base.The concentration of the 6- benzylaminopurines, methyl α-naphthyl acetate, gibberellin and the inositol that contain in each group and combination are shown in Table 1.
After medium sterilization, choose seedling age No. 8 in vitro cuttings of Gansu Province potato of 20~30 days, under aseptic condition, cut 0.5cm~
1cm is inoculated on above-mentioned culture medium without the stem section of axillary bud.The blake bottle being inoculated with is placed on light culturing rack and cultivated, temperature
Spend for 21 ± 2 DEG C, intensity of illumination 3500LX, light application time 16h/d.Cultivate 30d or so statistics inductivities（Differentiation rate %=differentiation
Explant number/total explant number）, the results are shown in Table 2.
Note：Different lowercase letter indication differences in same column are notable,P<0.05；Capitalization represents that difference is extremely notable,P<
0.01
The experimental data of the gained of analytical table 2 understands that 4 kinds of compositions are to the ability of No. 8 induced bud differentiation of Gansu Province potato by being followed successively by weak by force：
6-BA>Inositol>GA3>NAA, it can be seen that formation of the 6-BA presence on adventitious bud influences maximum, and the influence of inositol is taken second place, GA3With
NAA influence is weaker.To seek the hormone combinations of optium concentration, variance analysis is carried out, is as a result shown, nothing between combination 6,8,16
Pole significant difference, but pole is noticeably greater than other combinations, wherein combination 6 and 8 is without significant difference, 6 and 16 significant differences, 8 and 16
Without significant difference；Between combination 8,16,9 and 14, between 16,9,14 and 11, between 9,14,11 and 10, between 10,13 and 4,
13rd, between 4,15 and 5, electrodeless significant difference between 4,15,5,2 and 12, but significant difference, and between combining 2,12 and 7 with
And both electrodeless significant difference or without significant difference between 7,1,3.To sum up explanation combination 6 and 8 is to promote adventitious bud formation most
Excellent combination, and from the point of view of the growing way of Multiple Buds, the Multiple Buds quantity of the induction of combination 8 is more and growth potential is stronger.
In summary, the nutrient media components that can make potato stem section isolated culture one-step seedling is：Using MS culture mediums as
Basal medium, and in 1L MS culture mediums, also needs the final concentration of of the component added and each component：
2.5~7.5mg/L of gibberellin；
1~3mg/L of 6- benzylaminopurines；
0.01~0.1mg/L of methyl α-naphthyl acetate；
Inositol 50-100mg/L.
In conjunction with table 2 analyze the 8th group corresponding to culture medium in each composition concentration and combination, it is known that, optimal horse
The component of bell potato one-step culture base is：In 1L MS culture mediums, contain final concentration of 5mg/L gibberellin, 3mg/L 6- benzyls
Base adenine phosphate, 100mg/L inositols and 0.01mg/L methyl α-naphthyl acetates.
Embodiment 2
Using described potato one-step culture base, make potato stem section tissue cultures forming seedling through one step culture, comprise the following steps：
(1), added in every liter of MS culture medium 1mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 2.5mg gibberellin, 50mg inositols,
25g edible sugars and 3g agar；PH value is adjusted to 5.8；High pressure steam sterilization after packing.Culture medium after sterilizing is put into inoculation
Indoor standing, allows it to be solidified after cooling.
（2）Choose under the seedling age Potato Cultivars Atlantic Ocean in vitro cuttings of 20~30 days, aseptic condition, cut 0.5cm
~1cm is inoculated on above-mentioned culture medium without the stem section of axillary bud.The blake bottle being inoculated with is placed on light culturing rack and cultivated,
Temperature is 21 ± 2 DEG C, intensity of illumination 3500LX, light application time 16h/d.
（3）28d is cultivated, potato regeneration plant is obtained.
Embodiment 3
Added in every liter of MS culture medium 3mg 6- benzylaminopurines, 0.1mg methyl α-naphthyl acetates, 7.5mg gibberellin, 100mg inositols,
30g edible sugars and 4.5g agar；Culture medium is heated to being stirred continuously in boiling, heating process, will after agar is completely dissolved
PH value is adjusted to 6.0；15~20min is sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa,
Culture medium after sterilizing stands cooling solidification；Under aseptic condition, stem sections of the 0.5cm~1cm without axillary bud is cut, step is inoculated in
B culture medium；By step C be inoculated with stem section culture medium be placed in temperature for 21 ± 2 DEG C, intensity of illumination 4000LX, light application time
Cultivated under conditions of 16h/ days；Choose the seedling age Potato Cultivars LK99 of 20~30 days, one-step culture basigamy system and culture
Method ibid, cultivates 40d, obtains potato regeneration plant.
Embodiment 4
3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg gibberellin, 100mg inositols 30g are added in every liter of MS culture medium
Edible sugar and 4.5g agar；Culture medium is heated to being stirred continuously in boiling, heating process, by pH value after agar is completely dissolved
It is adjusted to 6.0；15~20min, sterilizing are sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa
Culture medium afterwards stands cooling solidification；Under aseptic condition, stem sections of the 0.5cm~1cm without axillary bud is cut, is inoculated in step B's
Culture medium；By the step C culture mediums for being inoculated with stem section be placed in temperature for 21 ± 2 DEG C, intensity of illumination 4000LX, light application time 16h/ days
Under conditions of cultivate；
Seedling age Potato Cultivars Gansu Province potato 7 of 20~30 days is chosen, one-step culture basigamy system and cultural method ibid, are cultivated
32d, obtains potato regeneration plant.
Embodiment 5
2mg 6- benzylaminopurines, 0.05mg methyl α-naphthyl acetates, 5mg gibberellin, 70mg inositols, 26g are added in every liter of MS culture medium
Edible sugar and 4g agar；Culture medium is heated to being stirred continuously in boiling, heating process, adjusts pH value after agar is completely dissolved
Whole is 6.0；15~20min is sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa, after sterilizing
Culture medium stand cooling solidification；Under aseptic condition, stem sections of the 0.5cm~1cm without axillary bud is cut, step B training is inoculated in
Support base；By step C culture mediums for being inoculated with stem section be placed in temperature for 21 ± 2 DEG C, intensity of illumination 3800LX, light application time 16h/ days
Under the conditions of cultivate；Choose seedling age Potato Cultivars Gansu Province potato 12 of 20~30 days, one-step culture basigamy system and cultural method
Ibid, 30d is cultivated, potato regeneration plant is obtained.
Embodiment 6
3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg gibberellin, 100mg inositols 30g are added in every liter of MS culture medium
Edible sugar and 4.5g agar；Culture medium is heated to being stirred continuously in boiling, heating process, by pH value after agar is completely dissolved
It is adjusted to 6.0；15~20min, sterilizing are sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa
Culture medium afterwards stands cooling solidification；Under aseptic condition, stem sections of the 0.5cm~1cm without axillary bud is cut, is inoculated in step B's
Culture medium；By the step C culture mediums for being inoculated with stem section be placed in temperature for 21 ± 2 DEG C, intensity of illumination 4000LX, light application time 16h/ days
Under conditions of cultivate；
Seedling age Potato Cultivars Gansu Province potato 9 of 20~30 days is chosen, one-step culture basigamy system and cultural method ibid, are cultivated
30d, obtains potato regeneration plant.
Embodiment 7
3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg gibberellin, 100mg inositols 30g are added in every liter of MS culture medium
Edible sugar and 4.5g agar；Culture medium is heated to being stirred continuously in boiling, heating process, by pH value after agar is completely dissolved
It is adjusted to 6.0；15~20min, sterilizing are sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa
Culture medium afterwards stands cooling solidification；Under aseptic condition, stem sections of the 0.5cm~1cm without axillary bud is cut, is inoculated in step B's
Culture medium；By the step C culture mediums for being inoculated with stem section be placed in temperature for 21 ± 2 DEG C, intensity of illumination 4000LX, light application time 16h/ days
Under conditions of cultivate；
Seedling age Potato Cultivars Gansu Province potato 5 of 20~30 days is chosen, one-step culture basigamy system and cultural method ibid, are cultivated
40d, obtains potato regeneration plant.
Embodiment 8
3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg gibberellin, 100mg inositols 30g are added in every liter of MS culture medium
Edible sugar and 4.5g agar；Culture medium is heated to being stirred continuously in boiling, heating process, by pH value after agar is completely dissolved
It is adjusted to 6.0；15~20min, sterilizing are sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa
Culture medium afterwards stands cooling solidification；Under aseptic condition, stem sections of the 0.5cm~1cm without axillary bud is cut, is inoculated in step B's
Culture medium；By the step C culture mediums for being inoculated with stem section be placed in temperature for 21 ± 2 DEG C, intensity of illumination 4000LX, light application time 16h/ days
Under conditions of cultivate；
Choose seedling age Potato Cultivars Gansu Province potato 3 of 20~30 days, Gansu Province potato 4, Gansu Province potato 11, Gansu Province potato 14, forming seedling through one step culture training
The basigamy system of supporting and cultural method ibid, cultivate 35d, obtain potato regeneration plant.
Claims (3)
1. a kind of potato stem section tissue culture medium (TCM) and its cultural method, it is characterised in that comprise the following steps：
It is prepared by culture medium
1~3mg 6- benzylaminopurines, 0.01~0.1mg methyl α-naphthyl acetates, 2.5~7.5mg are added in every liter of MS culture medium red mould
Element, 50-100mg inositols, 25~30g edible sugars and 3~4.5g agar；
B, medium treatment
Culture medium is heated to being stirred continuously in boiling, heating process, and pH value is adjusted into 5.8~6.0 after agar is completely dissolved；
High pressure steam sterilization after packing, the culture medium after sterilizing stands cooling solidification；
Connect seedling process
Choose under the seedling age potato in vitro cuttings of 20~30 days, aseptic condition, cut stems of the 0.5cm~1cm without axillary bud
Section, is inoculated in step B culture medium；
Connect seedling culture
By step C be inoculated with stem section culture medium be placed in temperature for 21 ± 2 DEG C, 3500~4000LX of intensity of illumination, light application time
Cultivated under conditions of 16h/ days；
E, subsection filter
Culture 15 days, stem section is expanded, and has Callus formation, and surface forms particulate material or strumae；Culture 25~30
My god, callus from stem segment differentiation Multiple Buds；Culture 28-35 days, obtains potato regeneration plant.
2. a kind of potato stem section tissue culture medium (TCM) according to claim 1 and its cultural method, it is characterised in that described
Culture medium is heated to boiling in step B, is stirred continuously in heating process, pH value is adjusted to 5.8 after agar is completely dissolved~
6.0；15~20min, the training after sterilizing are sterilized under the conditions of high pressure steam sterilization after packing, 120 DEG C of vapor (steam) temperature, pressure 1.5Mpa
Support base and stand cooling solidification.
3. a kind of potato stem section tissue culture medium (TCM) according to claim 1 and its cultural method, it is characterised in that described
3mg 6- benzylaminopurines, 0.01mg methyl α-naphthyl acetates, 5mg gibberellin, 100mg inositols are added in every liter of MS culture medium.
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| CN107821167A (en) * | 2017-11-29 | 2018-03-23 | 百色学院 | A kind of method for tissue culture of drinamyl potato |
| CN108401912A (en) * | 2018-06-19 | 2018-08-17 | 福建农林大学 | A method of probing into hormon and additive influences potato growth |
| CN111011217A (en) * | 2019-12-31 | 2020-04-17 | 青海省农林科学院 | Efficient regeneration method of heterozygous diploid potato RH |
| CN111011216A (en) * | 2019-12-31 | 2020-04-17 | 青海省农林科学院 | Efficient regeneration culture medium and culture method for Atlantic potatoes |
| CN114271190A (en) * | 2022-02-18 | 2022-04-05 | 甘肃省农业科学院马铃薯研究所 | Culture medium for potato tissue culture and application thereof |
| CN116491417A (en) * | 2023-03-29 | 2023-07-28 | 云南师范大学 | Regeneration method of potato wild species S.commersonii |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821167A (en) * | 2017-11-29 | 2018-03-23 | 百色学院 | A kind of method for tissue culture of drinamyl potato |
| CN107821167B (en) * | 2017-11-29 | 2019-08-06 | 百色学院 | A kind of method for tissue culture of drinamyl potato |
| CN108401912A (en) * | 2018-06-19 | 2018-08-17 | 福建农林大学 | A method of probing into hormon and additive influences potato growth |
| CN111011217A (en) * | 2019-12-31 | 2020-04-17 | 青海省农林科学院 | Efficient regeneration method of heterozygous diploid potato RH |
| CN111011216A (en) * | 2019-12-31 | 2020-04-17 | 青海省农林科学院 | Efficient regeneration culture medium and culture method for Atlantic potatoes |
| CN111011216B (en) * | 2019-12-31 | 2023-04-28 | 青海省农林科学院 | Efficient regeneration culture medium and culture method for atlantic potatoes |
| CN111011217B (en) * | 2019-12-31 | 2023-04-28 | 青海省农林科学院 | Efficient regeneration method of hybrid diploid potato RH |
| CN114271190A (en) * | 2022-02-18 | 2022-04-05 | 甘肃省农业科学院马铃薯研究所 | Culture medium for potato tissue culture and application thereof |
| CN114271190B (en) * | 2022-02-18 | 2023-01-13 | 甘肃省农业科学院马铃薯研究所 | Culture medium for potato tissue culture and application thereof |
| CN116491417A (en) * | 2023-03-29 | 2023-07-28 | 云南师范大学 | Regeneration method of potato wild species S.commersonii |
| CN116491417B (en) * | 2023-03-29 | 2024-04-16 | 云南师范大学 | Regeneration method of potato wild species S.commersonii |
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