CN107045188B

CN107045188B - Reflective microscope module and reflective microscope device

Info

Publication number: CN107045188B
Application number: CN201710040411.7A
Authority: CN
Inventors: 林建明; 陈昌佑; 蒋存超; 林书圣
Original assignee: Aidmics Biotechnology Co ltd
Current assignee: Aidmics Biotechnology Co ltd
Priority date: 2016-02-05
Filing date: 2017-01-20
Publication date: 2020-03-17
Anticipated expiration: 2037-01-20
Also published as: JP6745734B2; CN107045188A; JP6585642B2; CN107045191A; TW201728956A; JP2017138598A; TWI664416B; CN107045191B; TW201802449A; JP6521331B2; TWI644124B; CN107045190A; AU2017200620A1; JP2017160419A; JP2017138603A

Abstract

The present invention relates to a reflection type microscope module and a reflection type microscope apparatus. The invention discloses a reflection type microscope module which is matched with an image capturing module for use. The reflective microscope module comprises a shell, a lens and a sample adhering piece. The casing has a sample observation face, and the sample observation face is located the casing and for one side of image acquisition module, and lens set up in the casing inside, and the sample is adhered a piece and is set up in a bottom of casing removably, and the sample is adhered a piece and is adjoint the sample observation face and include a substrate and a glue film, and the glue film combines as an organic wholely with the substrate. The invention further discloses a reflection type microscope device.

Description

Reflective microscope module and reflective microscope device

Technical Field

The invention relates to a reflection type microscope module and a reflection type microscope device.

Background

Microscopes can be classified into transillumination microscopes and reflection microscopes (also called metallographic microscopes). The transillumination microscope is usually used to observe a transparent or very thin sample object, so that the light from the light source can directly pass through the sample object and enter the microscope, and is often used to observe biological tissues. The reflective microscope is often used to observe opaque sample objects such as metals and minerals, and is often applied in the fields of engineering and materials. The light source of the reflective microscope must transmit the polarizer to form polarized light, turn part of the light to vertical downward to be projected on the surface of the sample object through the lens, then reflect the light from the surface of the sample object, and sequentially transmit the objective lens, the polarizer, the plane glass and the ocular lens to be amplified, and enter the eyes of the observer, which can be used for observing the characteristics of the surface of the sample object.

However, the objective lens, the polarizer, the plane glass and the eyepiece are required to be arranged inside the reflex microscope, and particularly, the polarizer must have a specific angle design, so that the reflex microscope has a larger volume. Meanwhile, the mechanism of the reflex microscope is complicated and difficult to carry, so the reflex microscope is often placed in a laboratory and is usually operated and executed by a professional. Moreover, the reflective microscope is mainly used to observe the surface characteristics of a sample object, and if the sample object to be observed is encountered, the sample object must be sampled to a laboratory for observation, which causes inconvenience to users and is difficult to be applied to non-professional ordinary users. In other words, the reflective microscope is usually used to observe the surface characteristics of the sample object, so it is more desirable for the user to carry the microscope. In addition, if the sample that the user collected, can't select suitable sample to glue the piece to install it simply and set up in microscope and observe, will reduce the flexibility and the elasticity that the microscope is suitable for sample to glue the piece by a wide margin, also can't promote the user to operating microscope's use enjoyment.

Therefore, in addition to being light and portable, the reflex microscope should be capable of obtaining a sample in a simple and fast way, and the sample after sampling should also be capable of being fixed on the microscope in a simple way for the user to observe. Therefore, the difficulty of microscope operation is reduced, and the user can intuitively and easily operate the microscope without executing detection related operations only through a professional. And the user can simply install the collected sample on the microscope through the proper sample adhesion piece, so that the flexibility and the elasticity of the sample adhesion piece suitable for the microscope are improved. While increasing the willingness of the user to operate the microscope.

Disclosure of Invention

In view of the above, the present invention provides a light and portable reflective microscope module and a reflective microscope apparatus, wherein the sample adhering member is removably disposed at a bottom of the reflective microscope module housing, and the sample adhering member is adjacent to the sample observation surface, so that the sample can be easily and quickly fixed and disposed on the microscope for convenient observation, thereby reducing the difficulty of operation of the microscope and making the user intuitively and easily operate the microscope, and improving the desire of the user for operating the microscope.

To achieve the above object, the present invention provides a reflective microscope module, which is matched with an image capturing module. The reflective microscope module comprises a shell, a lens and a sample adhering piece. The shell is provided with a sample observation surface, and the sample observation surface is positioned on one side of the shell relative to the image acquisition module. The lens is arranged inside the shell. The sample adhesion piece is removably arranged at the bottom of the shell, is adjacent to the sample observation surface and comprises a substrate and an adhesive layer, and the adhesive layer and the substrate are combined into a whole.

In one embodiment, after a light beam is reflected by the sample adhering member, the light beam passes through the lens to reach the image capturing module.

In one embodiment, light emitted from a light source is incident from a light incident surface of the lens and exits from a light exiting surface of the lens to the sample observation surface.

In one embodiment, the reflective microscope module further comprises a light emitting element having a light source and disposed adjacent to the lens.

In one embodiment, the light source of the light emitting device is an annular light emitting body.

In an embodiment, the reflective microscope module further includes a light guide element disposed outside the housing, the light guide element having an incident portion and an emergent portion, the emergent portion being disposed adjacent to the lens, such that the incident portion receives a light and the emergent portion emits the light to the lens.

In one embodiment, when the reflective microscope module is not used with a light source, the housing has at least one hole for external light to enter the interior of the housing and the sample observation surface.

In one embodiment, the substrate has a recess and an extension portion, the extension portion is adjacent to the recess, and the adhesive layer is disposed on one side of the substrate having the recess.

In one embodiment, the sample adhering member further comprises a carrier, and the sample adhering member is removably adhered to a surface of the carrier, so that the concave portion and the surface form a containing space.

In one embodiment, the viscosity of the adhesive layer in the concave part is lower than that of the adhesive layer in the extending part.

In one embodiment, at least a portion of the sample-adhering member is a light-transmitting region.

In one embodiment, the extension portion is an opaque region and the recess portion is a transparent region.

In one embodiment, the recess has a light-shielding point.

In one embodiment, the sample adhering member is a sticker.

In one embodiment, the carrier is a glass slide, a plastic sheet, a resin sheet, a light transmissive sheet, or a light opaque sheet.

In one embodiment, the housing is removably fixedly connected to the sample adhering member.

In one embodiment, the reflex microscope module further comprises a detachable cover, and the housing and the detachable cover are connected by a screw, a fastening unit or a magnetic unit in a manner of screw locking, fastening or magnetic attraction.

In one embodiment, the bottom has a sample-adhering member insertion slot, and the sample-adhering member is disposed on the bottom through the sample-adhering member insertion slot.

In one embodiment, the sample-adhering member insertion slot has at least one stopper.

In one embodiment, the position limiting strip is provided with at least one magnetic unit, the sample adhering piece is removably adhered to a carrier, and the sample adhering piece adhered to the carrier and the sample adhering piece insertion groove are magnetically connected with each other through the position limiting strip provided with the magnetic unit.

In one embodiment, the sample-adhering member insertion slot has a transparent region or an open region.

In one embodiment, the bottom portion has two sample adhesive fixing structures.

In one embodiment, the fixing structure of the sample adhesion member is a magnetic unit, the sample adhesion member is removably adhered to a carrier, and the sample adhesion member adhered to the carrier is magnetically attracted to the bottom through the magnetic unit.

In one embodiment, the sample adhesion member is removably adhered to a carrier, and at least one of the housing and the carrier is provided with a magnetic unit, so that the sample adhesion member adhered to the carrier and the bottom can be magnetically connected with each other.

In one embodiment, the outer surface of the housing has a slope.

In an embodiment, the reflective microscope module further includes a connecting member disposed in the housing for connecting the image capturing module.

In one embodiment, the connecting member includes a connecting clip and a pivot.

In one embodiment, the shortest distance from the observation surface of the sample to the lens is between 0.1mm and 10 mm.

In one embodiment, the substrate has a flat surface without recesses.

To achieve the above object, the present invention further provides a reflective microscope device, which includes an image capturing module and the reflective microscope module.

In summary, the reflective microscope module of the present invention is used in combination with an image capturing module. The reflective microscope module comprises a shell, a lens and a sample adhering piece. The casing has a sample observation face, and the sample observation face is located the casing and for one side of image acquisition module, and lens set up in the casing inside, and the sample is adhered a piece and is set up in a bottom of casing removably, and the sample is adhered a piece and is adjoint the sample observation face and include a substrate and a glue film, and the glue film combines as an organic wholely with the substrate. The invention can conveniently and quickly fix the sample on the microscope to facilitate the observation of a user through the structural design that the sample adhesion piece is arranged at the bottom of the shell of the reflection type microscope module in a removable way, so that the sample adhesion piece is adjacent to the sample observation surface, the operation difficulty of the microscope is reduced, the user can intuitively and easily operate the microscope, and the intention of the user on operating the microscope is improved.

Drawings

Fig. 1A is a perspective view of a reflective microscope module according to an embodiment of the invention.

FIG. 1B is a cross-sectional view of the reflective microscope module shown in FIG. 1A.

Fig. 1C to 1D are schematic diagrams of the reflection microscope module and the image capturing module shown in fig. 1A before and after being combined.

FIG. 1E is a schematic cross-sectional view of the sample observed by the reflex microscope module shown in FIG. 1A.

FIG. 1F is a schematic view of the sample mount of the reflective microscope module shown in FIG. 1A.

FIG. 1G is a schematic cross-sectional view taken along line AA in FIG. 1F.

FIG. 1H is a detailed view of the sample-adhering part shown in FIG. 1A.

Fig. 2A to 2B are schematic cross-sectional views illustrating variations of a concave portion of a sample-adhering member according to an embodiment of the present invention.

FIG. 2C is a cross-sectional view of a sample-adhering member according to an embodiment of the present invention, showing different variations.

FIG. 2D is a cross-sectional view of the reflective microscope module shown using the sample mount of FIG. 2C.

Fig. 3A to 3B are schematic top views of a sample adhesive according to an embodiment of the invention.

FIG. 4A is a perspective view of a reflective microscope module with a removable lid according to an embodiment of the present invention.

FIG. 4B is a schematic view of the reflex microscope module shown in FIG. 4A with the housing and the removable lid attached.

FIG. 5A is a perspective view of the reflective microscope module with a sample-adhering member insertion slot at the bottom thereof according to one embodiment of the present invention.

FIG. 5B is a schematic view of the reflective microscope module of FIG. 5A at another angle.

Fig. 6A is a perspective view of a reflective microscope module with two sample adhesive fixing structures at the bottom thereof according to an embodiment of the invention.

FIG. 6B is a schematic view of the reflective microscope module of FIG. 6A at another angle.

Fig. 7A is a perspective view of the housing and the sample adhering member of the reflective microscope module with the magnetic unit according to an embodiment of the invention.

FIG. 7B is a schematic view of the reflective microscope module of FIG. 7A at another angle.

Fig. 7C is a perspective view of a housing of a reflective microscope module having at least one hole according to an embodiment of the invention.

Fig. 8A is an external perspective view of a reflective microscope module housing with a light guide assembly disposed thereon according to an embodiment of the invention.

Fig. 8B is a schematic cross-sectional view of the observation sample of the reflex microscope module shown in fig. 8A.

Detailed Description

A reflection microscope module and a reflection microscope apparatus according to preferred embodiments of the present invention will be described below with reference to the accompanying drawings, in which like elements are described with like reference numerals.

Referring to fig. 1A to 1D, fig. 1A is an external perspective view of a reflective microscope module according to an embodiment of the invention. FIG. 1B is a cross-sectional view of the reflective microscope module shown in FIG. 1A. Fig. 1C to 1D are schematic diagrams of the reflection microscope module and the image capturing module shown in fig. 1A before and after being combined. Please refer to fig. 1A to 1D. The reflex microscope module 1 includes a housing 11, a lens 12, and a sample mount 13 (the sample mount 13 is shown only in a side view in fig. 1B). In addition, the reflective microscope module 1 of the present embodiment may further include a connecting member 14, and the connecting member 14 is disposed on the housing 11. As shown in fig. 1C and fig. 1D, the reflective microscope module 1 of the present embodiment can be used with an image capturing module 2 through a connector 14. The connecting member 14 includes a connecting clip 141 and a pivot 142, and a user can open a side of the connecting clip 141 close to the housing 11 by pressing one end of the connecting clip 141 in cooperation with the pivot 142, and clamp the reflective microscope module 1 on an electronic device E. In another embodiment, the connecting member 14 can also be an adhesive layer instead of the clip, and the adhesive layer can be a pressure sensitive adhesive (pressure sensitive adhesive), i.e. a pressure sensitive adhesive, which can be repeatedly adhered, so that the reflective microscope module 1 can be repeatedly adhered to the electronic device E through the adhesive layer.

The electronic device E can be, for example, a mobile communication device with a camera function, a smart phone, a tablet computer, a camera, a driving recorder, a video camera, a notebook computer, a microscope or a wearable electronic device, and the embodiment is described by taking the mobile communication device as an example. That is, the reflective microscope module 1 of the present embodiment can be directly used with the image capturing module 2 of the electronic device E through the connecting member 14.

As shown in fig. 1B, the housing 11 has a sample viewing surface F1, the sample viewing surface F1 is located on a side of the housing 11 opposite to the image capturing module 2, and the housing 11 further has a bottom 111, the bottom 111 is also located on a side of the housing 11 opposite to the image capturing module 2, i.e. on a side close to the sample adhering member 13. The lens 12 is disposed inside the housing 11, and the sample adhering member 13 is sticky, so that it can be removably disposed on the bottom 111 of the housing 11 after being stuck with a sample S to be tested, so that the sample adhering member 13 is adjacent to the sample observation surface F1. By abutting the sample adhering member 13 to the sample observation surface F1, and then imaging through the lens 12 and the image capturing module 2, the sample image of the sample S to be measured after being magnified by the reflective microscope module 1 can be observed. In addition, the housing 11 and the sample-adhering member 13 are removably and fixedly connected in the present embodiment, and other technical contents about the sample-adhering member 13 will be described in detail later.

It should be noted that the sample observation surface F1 in this embodiment may be a solid surface or a virtual surface. In terms of the physical surface, when the sample adhesion member 13 and the housing 11 contact each other, the sample observation surface F1 is substantially the surface of the housing 11 opposite to the image capturing module 2. Regarding the virtual surface, as shown in fig. 1B, when there is a slight distance between the sample S to be measured and the housing 11, the sample observation surface F1 is the surface of the bottom 111 adjacent to the sample adhesion member 13. In addition, the sample to be observed is not limited in the embodiment, and the sample S to be measured and the size and the ratio thereof listed in the drawings are only exemplary and not limiting.

Referring to fig. 1E, fig. 1E is a schematic diagram of the sample observed by the reflective microscope module shown in fig. 1A. The lens 12 of the present embodiment is an aspherical mirror with a biconvex lens, wherein, under the condition of high magnification or with the objective of having the depth of field effect, the shortest distance D from the sample viewing surface F1 to the surface of the lens 12 is between 0.1mm and 10mm, and may be between 0.1mm and 3.0mm in general, preferably between 0.3mm and 2.0mm, and more preferably, the shortest distance D may be between 0.5mm and 1.2 mm. The magnification of the reflective microscope module 1 can be 100 to 200 times. In the present embodiment, the lens 12 has a wing portion 121, and the wing portion 121 is located on the periphery of the lens 12. That is, the central portion of the lens 12 is a double-sided convex aspherical mirror, and the peripheral portion of the lens 12 is a flat wing 121. In addition, in other embodiments, the lens 12 used in the reflective microscope module may be not an aspherical lens of a biconvex lens, but a spherical lens, a single convex lens, a single/biconcave lens or a lens assembly combining a plurality of lenses may be used, which may be determined according to the observation requirement of the user, and the invention is not limited thereto.

In addition, the reflective microscope module 1 of the present embodiment may further include a light emitting element 15, wherein the light emitting element 15 has a light source 151 and is disposed adjacent to the lens 12. The light source 151 may be a light emitting diode, a laser diode, or a fluorescent lamp. The light source 151 of the present embodiment is located between the image capturing module 2 and the lens 12, specifically, the light source 151 of the present embodiment is disposed inside the housing 11, and the light source 151 is located at the side of the lens 12 close to the image capturing module 2. In another embodiment, the light source 151 of the light emitting assembly 15 may be an annular light emitter, and is similarly disposed adjacent to the lens 12 to emit light. After the light emitted from the light source 151 is reflected by the sample adhering member 13, the light can pass through the lens 12 to reach the image capturing module 2.

More specifically, by the arrangement relationship between the lens 12 and the light emitting element 15, the light emitted from the light source 151 can be incident from a light incident surface 122 of the lens 12, and can be emitted from a light emitting surface 123 of the lens 12 to the sample observation surface F1, the sample adhering member 13 and the sample S to be measured. In addition, the housing 11 of the present embodiment has a light exit hole 112 and an opening 113. The light exit hole 112 is located at the side close to the sample observing surface F1, and in this embodiment, the light exit hole 112 is equivalent to an opening or an open hole of the bottom 111, and the opening 113 is located at the side close to the image capturing module 2. Therefore, the whole path of the light emitted by the light source 151 is incident from the light incident surface 122 of the lens 12, and is emitted from the light emitting surface 123, and then is emitted to the sample observation surface F1, the sample adhering member 13 and the sample S to be measured through the light emitting hole 112. Then, after being reflected by the sample adhering member 13 and the sample observing surface F1, the light is incident from the light emitting surface 123 of the lens 12, and is emitted from the light incident surface 122 and enters the lens of the image capturing module 2 after passing through the opening 113. After the lens of the image capturing module 2 obtains the amplified sample image, the image processing program is executed through the image capturing module 2, and the display unit of the electronic device E can display the sample image, i.e., the sample image amplified by the reflective microscope module 1, so that a user can directly observe the sample image amplified by the reflective microscope module 1 at the end of the electronic device E. Therefore, the reflective microscope module 1 of the present embodiment belongs to a reflective microscope (metallographic microscope) due to the above-mentioned optical path structure.

Preferably, the light source 151 of the present embodiment is disposed in the housing 11, the light source 151 is disposed adjacent to the wing portion 121 of the lens 12, and the wing portion 121 is a portion irrelevant to imaging, so the present embodiment does not limit the length of the wing portion 121. The wing 121 is disposed around the lens 12, so the light source 151 is disposed around the central protrusion of the lens 12. The above-mentioned arrangement relationship enables the light emitted by the light source 151 to enter the lens 12 from the wing 121 for diffusion, and then to be focused on the focal point from the light emitting surface 123 of the lens 12, wherein the main optical axis of the light source 151 is different from the optical axis of the lens 12, but is substantially parallel to the optical axis.

In this embodiment, the light source 151 may be a visible light source or a non-visible light source. Where the visible light source may be used as a source for viewing most sample types, and the non-visible light source may be, for example, an infrared light source, which may be used for jewelry identification, or an ultraviolet light source, which may be used for identification of security labels, such as for currency detection. In addition, the present invention does not limit the number of the light sources 151, for example, the light emitting assembly 15 may have a plurality of light sources. Therefore, the reflective microscope module 1 can be selectively provided with the light sources 151 and the number of the light sources in different manners according to different requirements and different types of samples to be observed.

The details of the sample-adhering member and the related components of the present embodiment are further described below.

Please refer to fig. 1F to fig. 1H. FIG. 1F is a schematic view of the sample mount of the reflective microscope module shown in FIG. 1A. FIG. 1G is a schematic cross-sectional view taken along line AA in FIG. 1F. FIG. 1H is a detailed view of the sample-adhering part shown in FIG. 1A.

Referring to fig. 1F to fig. 1H, the sample adhering member 13 may further cooperate with a carrier 133 and be used with the reflective microscope module in the following embodiments. The carrier 133 may have a sample carrying surface F2, and the carrier 133 may be a glass slide, a plastic sheet, a resin sheet, a light-transmitting sheet, or a light-opaque sheet. The sample adhesive 13 includes a substrate 130 and a glue layer C (see fig. 1G and fig. 1H). The substrate 130 has a first surface a and a second surface B opposite to each other, and the adhesive layer C is disposed on the second surface B of the substrate 130. The substrate 130 has an extension 131 and a recess 132, and the extension 131 is adjacent to the recess 132. The adhesion part 13 of the adhesive layer C in the concave portion 132 has a lower viscosity than the adhesive layer C in the extending portion 131, and the adhesive layer C is disposed on the side (the second surface B) of the substrate 130 having the concave portion 132 and integrated with the substrate 130. In addition, the substrate 130 and the adhesive layer C of the present embodiment are light-permeable, and the light transmittance thereof is greater than 90%.

The sample adhering member 13 has viscosity on one side of the sample bearing surface F2, and when the extending portion 131 of the sample adhering member 13 is attached to the carrier 133, the recess 132 and the sample bearing surface F2 may form an accommodating space V for accommodating a sample S to be tested. The user only needs to hold or stick the sample S to be tested by the concave portion 132 of the sample sticking member 13 and stick the sample S to the carrier 133, thereby completing the preparation of the microscope sample, and greatly simplifying the preparation process of the conventional microscope sample.

The accommodating space V can seal a sample S to be detected and can prevent liquid from leaking. The sample S to be tested can be a microorganism, a cell, an arthropod or a mineral powder, etc. The sample S to be measured is not limited to a liquid sample or a living body sample. For example: the user can stick the living insects or hold the liquid specimen through the concave portion 132 of the sample sticking member 13, and then attach the extending portion 131 of the sample sticking member 13 to the sample carrying surface F2 of the carrier 133, so as to seal the living insects or the liquid specimen in the accommodating space V. Since the viscosity of the concave portion 12 is lower than that of the extending portion 131, the small live insects can still move in the accommodating space V without being pressed by the sample adhering member 13. If the user wants to store the sample S to be tested, the sample adhering member 13 can be peeled off from the carrier 133 and adhered to another plastic film or sticker for storage.

In another embodiment, the user can also store the sample to be collected into a paper card, so the sample adhering member 13 can also be a sample paper card, and the user does not need to additionally use a carrier because the sample paper card is directly used. The sample paper card is removably arranged at the bottom of the shell, so that the sample paper card is adjacent to the sample observation surface, and can be imaged through the lens and the image acquisition module, and the imaging of the sample to be detected on the sample paper card after the sample to be detected is amplified by the reflection type microscope module can be observed.

In addition, the reflective microscope module of the present embodiment can further use a sample adhering member in different ways, and cooperate with the carrier provided above.

Fig. 2A to 2B are schematic cross-sectional views illustrating variations of a concave portion of a sample-adhering member according to an embodiment of the present invention. Referring to fig. 1H and fig. 2A to 2B, the

sample adhesion pieces

13, 13a, 13B respectively have an

extension portion

131, 131a, 131B and a recess portion 132, 132A, 132B. The sample-adhering

members

13, 13a, 13b may be stickers, tapes or resins, and have extensibility. Therefore, a user can stamp the sample adhesion piece into the concave structures with different shapes by means of stamping. For example: the recess 132 of fig. 1H and the recess 132A of fig. 2A are different types of arc structures, and the recess 132B of fig. 2B is a square structure.

Referring to fig. 2C and 2D, fig. 2C is a schematic cross-sectional view of a sample adhesive member according to an embodiment of the invention in different variations. FIG. 2D is a cross-sectional view of the reflective microscope module shown using the sample mount of FIG. 2C. Referring to fig. 2C and 2D together, in fig. 2C, the sample adhesion member 13C also has a substrate 130C and a glue layer C, but is different from the sample adhesion members 13a and 13B of fig. 2A or 2B in that the substrate 130C of the sample adhesion member 13C does not have the aforementioned concave portions and extending portions, so in this embodiment, the substrate 130C has a flat surface without concave portions, and the glue layer C can be disposed on one side of the flat substrate 130C and also integrated with the substrate 130C. In addition, the substrate 130C and the adhesive layer C can also have high light transmittance, so that the sample adhesive 13C is an adhesive with high light transmittance and full flatness.

In addition, the reflection microscope module of fig. 2D has largely the same components and component relationships as the reflection microscope module 1 of fig. 1B. Except that the reflective microscope module shown in fig. 2D uses the sample holder 13C in the full flat manner shown in fig. 2C. The sample adhesion piece 13c is sticky, so that it can be removably arranged on the bottom 111 of the housing 11 after being stuck with a sample S to be tested, so that the sample adhesion piece 13c is adjacent to the sample observation surface F1. By abutting the sample adhering member 13c to the sample observing surface F1, and then imaging through the lens 12 and the image capturing module 2, the sample image of the sample S to be measured after being magnified by the reflective microscope module 1 can be observed.

In addition, other technical features of the reflection microscope module shown in fig. 2D can refer to the related description of the reflection microscope module 1, and are not described herein again.

In the following examples, the sample adhesive member 13 will be described by taking a sticker as an example. The sample adhesion member 13 can be a transparent sticker with a light transmittance of more than 90%, and a part of the sample adhesion member 13 can be shaded to form a dark-field sticker.

Fig. 3A to 3B are schematic top views of a sample adhesive according to an embodiment of the invention. Referring to fig. 3A, the concave portion 132 of the sample adhesion member 13 has a light-shielding point d, and the areas outside the light-shielding point d are light-transmitting areas. In the embodiment of fig. 3A, the light-shielding point d is a printing black dot, and the light-shielding point d may be disposed at any position on the surface of the concave portion 132, and is not necessarily disposed at the center of the concave portion 132, as long as the imaging background of the sample S to be measured exhibits the dark field effect. The recessed portion 132 of fig. 3A may also be provided with a plurality of dispersed printed black dots to form a light-shielding region. Therefore, only one or more dispersed printing black spots are required to be arranged in the originally whole transparent concave part 132 to form a shading area, only the refracted and scattered light is emitted to the sample S to be detected as far as possible, the dark field effect can be achieved, and the resolution is improved.

In another embodiment, as shown in FIG. 3B, only the recess 132 of the sample-adhering member 13 is a light-transmitting region, and the remaining regions are all opaque regions. Therefore, the sample adhesion member 13 can be a dark-field sticker by setting the non-recessed region of the sample adhesion member 13 as a light-shielding region. In addition, the modes of fig. 3A and 3B may also be used in combination. By means of the design mode of the dark-field paster, the contrast between the sample and the background can be improved, and further the optimal imaging effect is obtained.

Therefore, the reflective microscope module of the embodiment is matched with an image capturing module for use. The reflective microscope module comprises a shell, a lens and a sample adhering piece. The shell is provided with a sample observation surface, the sample observation surface is positioned on one side of the shell relative to the image acquisition module, the lens is arranged in the shell, the sample adhesion piece is removably arranged at the bottom of the shell, and the sample adhesion piece is adjacent to the sample observation surface and comprises a base material and an adhesive layer. The base material is provided with a concave part and an extending part, the extending part is adjacent to the concave part, and the glue layer is arranged on one side of the base material with the concave part and is combined with the base material into a whole. The housing 11 of the present embodiment is removably and fixedly connected to the sample-adhering member 13, so that the sample-adhering member is removably disposed at a bottom of the housing of the reflective microscope module. Through the design, the sample can be simply, conveniently and quickly fixed and arranged on a microscope convenient user for observation, the aim of reducing the operation difficulty of the microscope, enabling the user to intuitively and easily operate the microscope, avoiding improving the operation difficulty of the user and simultaneously achieving the aim of improving the willingness of the user to operate the microscope is fulfilled.

Referring to fig. 4A to 4B, fig. 4A is an external perspective view of a reflective microscope module with a detachable lid according to an embodiment of the invention. FIG. 4B is a schematic view of the reflex microscope module shown in FIG. 4A with the housing and the removable lid attached.

The reflective microscope module 1a of fig. 4A has most of the same components and component relationships as the reflective microscope module 1 of the previous embodiment. The difference is that the reflective microscope module 1a of the present embodiment further comprises a detachable lid 16.

In this embodiment, the housing 11 and the detachable flap 16 are connected to each other in a snap-fit manner by a snap unit 161 provided in the detachable flap 16. As shown in fig. 4B, the detachable lid 16 can be covered on the bottom 111 of the housing 11 by the locking unit 161, and the housing 11 and the detachable lid 16 can be further matched with a mark or label to facilitate the alignment of the locking unit 161. Therefore, since the detachable seat cover 16 covers the bottom 111 of the housing 11, the sample adhesion member 13 disposed at the bottom 111 of the housing 11 can have better flatness, and the image effect of the sample S to be measured observed by the reflective microscope module 1a can be improved. In other embodiments, the housing 11 and the detachable cover 16 can be connected to each other by a screw or a magnetic unit. This also enables the detachable lid 16 to be connected to the housing 11, which also improves the image of the sample S to be observed by the microscope module 1 a.

In addition, other technical features of the reflective microscope module 1a can refer to the related description of the reflective microscope module 1, and are not described herein again.

In addition, referring to fig. 5A to 5B, fig. 5A is an external perspective view of a reflective microscope module according to an embodiment of the invention, wherein the bottom of the reflective microscope module has a sample adhesion member insertion slot. FIG. 5B is a schematic view of the reflective microscope module of FIG. 5A at another angle.

The reflective microscope module 1b of fig. 5A has most of the same components and component relationships as the reflective microscope module 1 of the previous embodiment. The difference is that the bottom 111b of the reflective microscope module 1b of the present embodiment has a sample-adhering member insertion slot I. In addition, the sample-adhering member insertion slot may further have at least one stopper bar.

In this embodiment, the bottom 111b has a sample-adhering member insertion slot I, and the sample-adhering member insertion slot I further has two position-restricting strips L1, L2. The sample-adhering member 13 may be disposed at the bottom 111b through the sample-adhering member insertion groove I by being attached to the carrier 133. Specifically, the user can insert the sample-adhering member 13 into the sample-adhering member insertion slot I along one direction, and due to the arrangement of the position-limiting strips L1 and L2, the sample-adhering member 13 can be aligned relatively precisely during the process of inserting the sample-adhering member insertion slot I, and the sample-adhering member 13 will not move in another direction other than the insertion direction, for example, if the user inserts the sample-adhering member 13 into the sample-adhering member insertion slot I from left to right, the sample-adhering member 13 will not move in the up-down direction during the insertion process. In addition, the bottom 111b of the housing 11b may have an insertion cut-off surface F3 at the other end of the sample-adhering member insertion slot I. The insertion cut-off surface F3 is connected to the stopper strips L1, L2 so that the sample-adhering piece 13 does not move after abutting against the insertion cut-off surface F3 after being inserted into the sample-adhering piece insertion groove I.

In addition, the position-limiting strips L1 and L2 may be provided with at least one magnetic unit (not shown), so that the carrier 133 attached to the sample-adhering member 13 and the sample-adhering member insertion slot I can be magnetically connected to each other through the position-limiting strips provided with the magnetic unit, for example, the magnetic unit may be disposed at a position close to the insertion stop surface in the position-limiting strips L1 and L2, so that the sample-adhering member 13 can be aligned with the position-limiting strips L1 and L2 and simultaneously attracted to the insertion stop surface. In addition, the sample-adhering member insertion slot has a transparent region or an opening region T, and the transparent region or the opening region T can make the light beam incident from the sample observation surface F1 to the sample S to be measured of the sample-adhering member 13, and then reflected back to the sample observation surface F1, and then incident back to the lens 12 and the image capturing module 2 through the aforementioned optical path structure. Thus, the sample-adhering member 13 can be directly inserted into the sample-adhering member insertion groove I through the guidance of the stopper. In addition, the position-limiting strips L1 and L2 can assist in directly aligning and inserting the sample-adhering piece 13 into the bottom of the sample-adhering piece insertion slot I, and the sample-adhering piece 13 can move left and right and cannot be completely clamped. Therefore, the position of the sample S to be measured can be finely adjusted left and right by arranging the limit strips L1 and L2, so that the sample S to be measured can be conveniently moved to the position which is expected to be observed by a user.

In addition, other technical features of the reflective microscope module 1b can refer to the related description of the reflective microscope module 1, and are not described herein again.

In addition, referring to fig. 6A to 6B, fig. 6A is an external perspective view of a reflective microscope module according to an embodiment of the invention, wherein the bottom of the reflective microscope module has two sample adhesion piece fixing structures. FIG. 6B is a schematic view of the reflective microscope module of FIG. 6A at another angle.

The reflective microscope module 1c of fig. 6A has most of the same components and component relationships as the reflective microscope module 1 of the previous embodiment. The difference is that the bottom 111c of the reflective microscope module 1c of the present embodiment has two sample-adhering member fixing structures.

In this embodiment, the bottom 111c has two sample-adhering-member fixing structures H1 and H2, and the sample-adhering-member fixing structures H1 and H2 have hook-like configurations, so that the sample adhering member 13 can be removably disposed on the bottom 111c of the housing 11c by the two sample-adhering-member fixing structures H1 and H2 after being attached to the carrier 133, and the sample adhering member 13 can move left and right in a small range on the bottom 111c of the housing 11c, and cannot be completely clamped by the sample-adhering-member fixing structures H1 and H2. Therefore, the position of the sample S can be finely adjusted left and right by the two sample-adhering member fixing structures H1 and H2, so that the sample S can be conveniently moved to the position desired to be observed by the user. In addition, the sample-adhering member fixing structures H1 and H2 of the present embodiment may also be a magnetic unit, and the carrier 133 to which the sample-adhering member 13 is attached may also have a magnetic unit, so that the sample-adhering member 13 adhered to the carrier 133 can magnetically attract the two magnetic sample-adhering member fixing structures H1 and H2 and magnetically attract the two magnetic sample-adhering member fixing structures H1 and H2 to the bottom 111 c. Specifically, the sample-adhering member-fixing structures H1 and H2 may be made of a material having spontaneous magnetism or magnetic permeability, and the material having spontaneous magnetism may be an alloy, such as an alloy containing terbium ferrite (TbFe), gadolinium cobaltate (GdCo), dysprosium nickel (DyNi), neodymium iron boron (NdFeB), or a ferrite or an intermetallic compound; the magnetic material may be cobalt-nickel-chromium (Co-Ni-Cr), cobalt-chromium-tantalum (Co-Cr-Ta), cobalt-chromium-platinum (Co-Cr-Pt), or cobalt-chromium-platinum-boron (Co-Cr-Pt-B). Therefore, if the material is a spontaneous magnetic material, the material can have a magnetic force without an applied magnetic field. In addition, if the material is magnetic conductive, it should be induced by an external magnetic field (e.g. a magnet close to spontaneous magnetism) to generate magnetic force at all times. The sample adhesion member 13 and the sample adhesion member fixing structures H1 and H2 are not limited to be made of materials with spontaneous magnetism or magnetic permeability. In other words, the sample-adhering member 13 and the sample-adhering member fixing structures H1 and H2 may be made of either a self-magnetic material or a self-magnetic material and the other one is made of a magnetic material, so long as the sample-adhering member 13 and the sample-adhering member fixing structures H1 and H2 can generate magnetic force and attract each other.

In addition, other technical features of the reflective microscope module 1c can refer to the related description of the reflective microscope module 1, and are not described herein again.

In addition, referring to fig. 7A to 7C, fig. 7A is an external perspective view of a housing and a sample adhesion member of a reflective microscope module according to an embodiment of the invention, wherein the housing and the sample adhesion member are provided with a magnetic unit. FIG. 7B is a schematic view of the reflective microscope module of FIG. 7A at another angle. Fig. 7C is a perspective view of a housing of a reflective microscope module having at least one hole according to an embodiment of the invention.

The reflective microscope module 1d of fig. 7A has most of the same components and component relationships as the reflective microscope module 1 of the previous embodiment. The difference is that the outer surface of the housing 11d of the reflective microscope module 1d of the present embodiment has a slope, and at least one of the housing and the sample adhering member is further provided with a magnetic unit.

In this embodiment, the housing 11d of the reflex microscope module 1d has a different outer surface from those of the previous embodiments, and the outer surface of the housing 11d has a slope rather than a generally vertical outer surface. The case 11d and the carrier 133d of the sample adhesion member 13d used in cooperation with the reflex-type microscope module 1d are provided with magnetic units, respectively. Specifically, the housing 11d is provided with a magnetic unit M1 at a position closer to the sample-adhering member 13d, and the carrier 133d adhered to the sample-adhering member 13d is correspondingly provided with a magnetic unit M2 at a position closer to the housing 11 d. The magnetic unit M1 may be a material having spontaneous magnetism or magnetic permeability, like the magnetic unit M2, and the material having spontaneous magnetism is, for example, an alloy including terbium ferrite (TbFe), gadolinium cobaltate (GdCo), dysprosium nickel (DyNi), neodymium iron boron (NdFeB), or a material of ferrite or intermetallic compound; the magnetic material may be cobalt-nickel-chromium (Co-Ni-Cr), cobalt-chromium-tantalum (Co-Cr-Ta), cobalt-chromium-platinum (Co-Cr-Pt), or cobalt-chromium-platinum-boron (Co-Cr-Pt-B). Therefore, if the material is a spontaneous magnetic material, the material can have a magnetic force without an applied magnetic field. In addition, if the material is magnetic conductive, it needs to be induced by an external magnetic field (e.g. a magnet close to spontaneous magnetism) to generate magnetic force. The magnetic units M1 and M2 are not limited to be either spontaneous magnetic or magnetic conductive. In other words, the magnetic unit M1 and the magnetic unit M2 may be both made of a self-magnetic material, or one may be a self-magnetic material and the other may be a magnetic material, so long as the magnetic unit M1 and the magnetic unit M2 can generate a magnetic force therebetween and the sample adhesion piece 13d adhered to the carrier 133d and the bottom 111d are magnetically connected to each other.

It should be noted that the magnetic force of the magnetic units M1, M2 cannot be too large or too small, and when the magnetic force of the magnetic units M1, M2 is too large, it will be difficult for the user to separate the sample-adhering piece 13d and the carrier 133d from the bottom 111d of the housing 11 d. However, if the magnetic force of the magnetic units M1 and M2 is too small, the sample-adhering piece 13d and the carrier 133d cannot be reliably disposed at the bottom of the housing 11d, and the sample-adhering piece 13d cannot abut or cling to the sample observation surface F1. In addition, in different embodiments, when the housing 11d is made of metal material such as iron, steel or nickel, the sample-adhering member 13d and the housing 11d can directly attract each other through the magnetic unit M2 disposed on the carrier 133d, so that the sample-adhering member is magnetically attracted to the bottom of the housing 11d, and there is no need to additionally dispose the magnetic unit M1 on the periphery of the housing 11 d. Therefore, compared with the prior reflection type microscope module, in the reflection type microscope module of the embodiment, the shell and the sample adhesion piece are magnetically connected to the bottom of the shell without being limited by the slot and the limiting strip due to the fact that at least one of the shell and the sample adhesion piece is provided with the magnetic unit. Through the design, the sample adhesion part has larger moving force and activity elasticity, and especially when the sample S to be measured does not belong to a completely static observation target, such as a living body, the reflection type microscope module of the embodiment can be further convenient for a user to observe.

Referring to fig. 7C, the reflective microscope module of fig. 7C and the reflective microscope module 1 of fig. 7A have most of the same components and relationships between the components. The difference is that the housing 11e of the reflective microscope module 1e of the present embodiment is not provided with a light emitting component, in other words, the reflective microscope module 1e is not an active light emitting microscope. That is, when the reflective microscope module 1e is not used with a light source (i.e. the housing 11e is not provided with a light emitting component), the housing 11e of the present embodiment may have at least one hole O for allowing external light to enter the interior of the housing 11e and the sample observation surface, and the present invention does not limit the size and number of the holes O disposed on the housing 11e, as long as the holes O are disposed to allow external light to enter the optical path structure via the housing 11e, and finally enter the lens of the image capturing module 2. Therefore, even if the reflective microscope module does not actively emit light, a user can introduce light from the outside by using the design of the shell with the holes, and can clearly observe the sample image amplified by the reflective microscope module.

In addition, other technical features of the reflective microscope modules 1d and 1e can refer to the related description of the reflective microscope module 1, and are not described herein again.

Referring to fig. 8A and 8B, fig. 8A is an external perspective view of a light guide assembly disposed outside a housing of a reflective microscope module according to an embodiment of the invention. Fig. 8B is a schematic cross-sectional view of the observation sample of the reflex microscope module shown in fig. 8A.

The reflective microscope module 1f of fig. 8 has most of the same components and component relationships as the reflective microscope module 1 of the previous embodiment. The difference is that the reflective microscope module 1f of the present embodiment does not have a light emitting element, but a light guide element is provided outside the housing.

Referring to fig. 8A and 8B, in the reflective microscope module 1f of the present embodiment, a light guide element 17 is disposed outside the housing 11, instead of disposing a light emitting element in the housing 11, and the light guide element 17 is disposed adjacent to the convex lens 12, so that the light source outside the housing 11 of the reflective microscope module 1f is guided to the convex lens 12 by the light guide element 17. Specifically, the light guide assembly 17 of the present embodiment has an incident portion 171 and an emergent portion 172, and the emergent portion 172 is located between the image capturing module 2 and the convex lens 12. The light incident portion 171 is used for receiving a light, wherein the light may be from the ambient light or from a flash lamp 22 of the image capturing module 2. In the embodiment, the light of the flash 22 of the image capturing module 2 is received as an example, so the light incident portion 171 is disposed adjacent to the flash 22 of the image capturing module 2. And preferably, the top surface of the light incident portion 171 may have an opening to receive light from the flash 22 or the environment. When the light incident portion 171 of the light guide assembly 17 receives the light from the flash 22, the light is emitted to the convex lens 12 through the light emitting portion 172.

In detail, the overall appearance of the light guiding assembly 17 may be a strip-shaped or ring-shaped structure, for example, the light incident portion 171 of the present embodiment receives light from the flash 22, so the disposition position of the light incident portion 171 needs to correspond to the flash 22, and the disposition position of the light emitting portion 172 corresponds to the lens 21 of the image capturing module 2. Since the lens 21 and the flash 22 of the image capturing module 2 are usually disposed adjacent to each other at a distance, the light guide assembly 17 has a strip-shaped appearance. Of course, in other embodiments, if the light incident portion 171 receives the ambient light, the overall appearance of the light guide assembly 17 may be a ring-shaped structure, and the invention is not limited thereto.

Preferably, the light incident portion 171 may have a hemispherical or arc-shaped structure, and may be designed to receive a wide range of light sources. Preferably, the light guide element 17 has a groove 173, as shown in fig. 8B, the groove 173 has a first inclined plane 174 and a second inclined plane 175, and the first inclined plane 174 and the second inclined plane 175 form an angle θ, which is between 45 and 120 degrees. By the design of the groove 173, the light received by the light-entering portion 171 is effectively guided to the light-exiting portion 172, and is emitted to the convex lens 12 from the light-exiting portion 172. Preferably, as shown in fig. 8A and 8B, the light guiding element 17 may have a light shielding layer 176, which may be a dark paint, coated between the light incident portion 17 and the light emergent portion 172 to prevent stray light in the environment from entering the light emergent portion 172.

With the above arrangement, the light emitted from the light emitting portion 172 can be incident from the light incident surface 122 of the convex lens 12 and then emitted from a light emitting surface 123 of the convex lens to the sample observing surface F1. Correspondingly, the housing 11 of the present embodiment also has a light exit hole 112 and an opening 113. The light exit hole 112 is located at the side close to the sample observation plane F1, and the opening 113 is located at the side close to the image acquisition module 2. Therefore, the entire path of the light emitted from the light emitting portion 172 is incident from the light incident surface 122 of the convex lens 12, and is emitted from the light emitting surface 123, and then is emitted to the sample observation surface F1 through the light emitting hole 112. Then, the light is reflected from the sample observation plane F1, then enters from the light emitting surface 123 of the convex lens 12, exits from the light incident surface 122, passes through the opening 113 and the light emitting part 172, and enters the lens 21 of the image capturing module 2. After the lens 21 of the image capturing module 2 obtains the amplified sample image, the image processing program is executed through the image capturing module 2, and the display unit of the electronic device E can display the sample image, i.e., the sample image amplified by the reflective microscope module 1, so that a user can directly observe the sample image amplified by the reflective microscope module 1 at the end of the electronic device E.

In addition, other technical features of the reflective microscope module 1f can refer to the related description of the reflective microscope module 1, and are not described herein again.

In addition, the present invention further provides a reflective microscope device, which includes an image capturing module and the reflective microscope module described in any of the above embodiments.

The foregoing is by way of example only, and not limiting. It is intended that all equivalent modifications or variations without departing from the spirit and scope of the present invention shall be included in the appended claims.

Claims

1. A reflective microscope module, which is used in combination with an image capturing module for observing a sample, comprises:

the shell is provided with a sample observation surface, and the sample observation surface is positioned on one side of the shell, which is opposite to the image acquisition module;

a lens disposed inside the housing; and

a sample adhering single piece removably disposed at a bottom of said housing, said sample adhering single piece abutting said sample viewing surface and comprising:

a monolithic substrate having a surface; and

a glue layer at least partially arranged on the surface of the single substrate and combined with the single substrate into a whole,

wherein the single piece of substrate of the sample-adhered single piece is removably disposed opposite the bottom of the housing via the glue layer to form an accommodating space between the single piece of substrate and the bottom of the housing for accommodating the sample, and the glue layer is disposed between the surface of the single piece of substrate and the bottom of the housing, both the glue layer and the sample being located on the same side of the single piece of substrate facing the lens.

2. The reflective microscope module of claim 1, wherein a light ray reflected by the sample adhering unit passes through the lens to the image capture module.

3. The reflective microscope module of claim 1, further comprising:

and the light-emitting component is provided with a light source and is arranged adjacent to the lens.

4. The reflective microscope module of claim 1, further comprising:

the light guide assembly is arranged outside the shell and provided with an incident part and an emergent part, and the emergent part is arranged adjacent to the lens, so that the incident part receives a light ray and the emergent part emits the light ray to the lens.

5. The reflective microscope module of claim 1, wherein the housing has at least one aperture for external light to enter the interior of the housing and the sample viewing surface when the reflective microscope module is not used with a light source.

6. The reflective microscope module of claim 1, wherein the monolithic substrate has a recess and an extension, the extension is adjacent to the recess, and the glue layer is disposed on a side of the monolithic substrate having the recess.

7. The reflectance microscope module according to claim 6, wherein the sample adhesion unit further comprises a carrier, the sample adhesion unit being removably adhered to a surface of the carrier such that the recess and the surface form the receiving space.

8. The reflective microscope module of claim 7, wherein at least a portion of the sample adhering unitary piece is a light transmissive region.

9. The reflective microscope module of claim 8, wherein the extension is an opaque region and the recess is a transmissive region.

10. A reflective microscope device, comprising:

an image capturing module; and

the reflective microscope module of one of claims 1 to 9.