CN107043648A - A kind of microbe fermentation method prepares the method and cyperus esculentus oil product of cyperus esculentus oil - Google Patents
A kind of microbe fermentation method prepares the method and cyperus esculentus oil product of cyperus esculentus oil Download PDFInfo
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- CN107043648A CN107043648A CN201710367406.7A CN201710367406A CN107043648A CN 107043648 A CN107043648 A CN 107043648A CN 201710367406 A CN201710367406 A CN 201710367406A CN 107043648 A CN107043648 A CN 107043648A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
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Abstract
The invention provides the method and cyperus esculentus oil product that a kind of microbe fermentation method prepares cyperus esculentus oil, belong to fats and oils processing field.This method includes：Cyperue esculentus powder is mixed with cushioning liquid, and in soaking 1.5~2.5h at 60~65 DEG C, carries out sterilization treatment, obtains sterile cyperue esculentus powder solution.Sterile cyperue esculentus powder solution is mixed with protease, cellulase, enzymolysis processing is carried out, obtains enzymolysis liquid.Inoculation includes the mixed bacteria of bacillus subtilis and trichoderma reesei in enzymolysis liquid, carries out aerobic fermentation, obtains zymotic fluid；And after zymotic fluid is handled through demulsification, centrifugation.This method is without organic solvent, and technique is simple, and without using large scale equipment, and grease extraction efficiency is high.Obtained cyperus esculentus oil, aromatic flavour, comfortable taste, oil is as clear as crystal, and oil quality is good.Cyperus esculentus oil product including cyperus esculentus oil made from the above method, quality is high, and mouthfeel is unique.
Description
Technical field
The present invention relates to the preparation field of cyperus esculentus oil, cyperue esculentus is prepared in particular to a kind of microbe fermentation method
The method and cyperus esculentus oil product of oil.
Background technology
Cyperue esculentus, also known as chufa, originate in African Mediterranean, belong to grass family annual plant.Cyperue esculentus is
A kind of high-quality, high yield, comprehensive utilization value very high oil, grain uses new crop, is also that beautification, greening environment view and admire plant
Thing.The varietal adaptation is strong, and yield is high, and extensive introduction of plant is on the Heilungkiang of China, Hebei and other places.
Cyperue esculentus is a kind of important oil crops, and it is to utilize one of main path of cyperue esculentus to produce oily sweetened bean paste oil.Pass
The extraction process of the cyperus esculentus oil of system is mainly milling process and extraction, and both approaches all cut both ways.Wherein, milling process,
Required corollary equipment is few, and technique is simple, and product can be made to keep original flavour, but this method residual oil content is high, cost is big, labor
Fatigue resistance is big, and the protein denaturation degree after squeezing in grouts is high；In extraction, resid amount is low, and production efficiency is high, but crude oil
Composition it is complex, it is necessary to which by further refining treatment, the flavor loss to product is big.In consideration of it, the present invention is proposed
A kind of new method for producing oily sweetened bean paste oil.
The content of the invention
The first object of the present invention is to provide a kind of method that microbe fermentation method prepares cyperus esculentus oil, and this method need not
Organic solvent, technique is simple, and without using large scale equipment, and grease extraction efficiency is high.Obtained cyperus esculentus oil, aromatic flavour,
Comfortable taste, oil is as clear as crystal, and oil quality is good.
The second object of the present invention is to provide a kind of product of cyperus esculentus oil, because the fragrance of cyperus esculentus oil is unique, oil
Lipid amount is good, and the quality of obtained cyperus esculentus oil product is high.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
A kind of method that microbe fermentation method prepares cyperus esculentus oil, it includes：
Cyperue esculentus powder is mixed with cushioning liquid, and in soaking 1.5~2.5h at 60~65 DEG C, carries out sterilization treatment, obtains
To sterile cyperue esculentus powder solution；
Sterile cyperue esculentus powder solution is mixed with protease, cellulase, and in carrying out enzymolysis processing at 70~75 DEG C, obtained
To enzymolysis liquid；
Inoculation includes the mixed bacteria of bacillus subtilis and trichoderma reesei in enzymolysis liquid, and in progress at 30~35 DEG C
Aerobic fermentation 2~3 days, obtains zymotic fluid；And
After zymotic fluid is handled through demulsification, centrifugation.
A kind of cyperus esculentus oil product, it is included as the cyperus esculentus oil obtained by the above method, and is subjected in bromatology
Additive.
Compared with prior art, beneficial effects of the present invention are：
The method that this microbe fermentation method that present disclosure is provided prepares cyperus esculentus oil, first using protease and fiber
Plain ferment treatment cyperue esculentus, destroys the cell membrane of cyperue esculentus, promotes grease to be discharged from cell；Pass through microbial fermentation again
Method, is added composite bacteria, it is fermented using the enzymolysis liquid of cyperue esculentus, produces a variety of flavor substances, zymotic fluid is demulsified
Obtained cyperus esculentus oil is centrifuged after processing.This method is without organic solvent, and technique is simple, without using large scale equipment, and grease
Recovery rate is high.Obtained cyperus esculentus oil, aromatic flavour, comfortable taste, oil is as clear as crystal, and oil quality is good.
A kind of product for cyperus esculentus oil that present disclosure is provided, because the fragrance of cyperus esculentus oil is unique, oil quality is good,
The quality of obtained cyperus esculentus oil product is high.
Embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the present invention.It is unreceipted specific in embodiment
Condition person, the condition advised according to normal condition or manufacturer is carried out.Agents useful for same or the unreceipted production firm person of instrument, be
The conventional products that can be obtained by commercially available purchase.
Present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
Step S1：Cyperue esculentus powder is mixed with cushioning liquid, and in soaking 1.5~2.5h at 60~65 DEG C, is carried out at sterilizing
Reason, obtains sterile cyperue esculentus powder solution.
Wherein, the preparation method of cyperue esculentus powder is：After cyperue esculentus is cleaned up, 10~30 mesh are crushed to, or be 15
~25 mesh, or be 20 mesh.Cyperue esculentus is crushed and granulated, is conducive to accelerating being swelled for cyperue esculentus, improves grease in cyperue esculentus
Dissolution efficiency.
Further, selected cushioning liquid is alkalescent, and its pH value is 7.5~8.0, or is 7.6~7.9, or
Person is 7.7~7.8.Weakly alkaline cushioning liquid, is conducive to dissolving after the pectin substance contained in cyperue esculentus stem tuber, and then reduction
Solution viscosity in continuous processing procedure, improves the recovery rate of grease.Selectable, cushioning liquid includes carbonate buffer solution
And/or phosphate buffer solution.
Further, the solid-liquid ratio of cyperue esculentus powder and cushioning liquid is 1：8~10, or be 1：9.Using this scope
Solid-liquid ratio, controls the concentration of cyperue esculentus powder, is easy to follow-up enzymolysis and fermentation process.
Optionally, sterilization treatment includes：By the mixed liquor of cyperue esculentus powder and cushioning liquid, high pressure is gone out at 110~120 DEG C
Bacterium handles 20~30min, or for by mixed liquor at 115 DEG C sterilization treatment 25min, to remove the bacterium in mixed liquor, true
The microorganisms such as bacterium.
Step S2：Sterile cyperue esculentus powder solution is mixed with protease, cellulase, and in lower carry out enzymolysis processing, obtained
To enzymolysis liquid.
Enzymolysis processing is carried out to sterile cyperue esculentus powder solution, protease and the cell of cellulose degraded cyperue esculentus is utilized
Wall, while the complexs such as lipoprotein of degrading, discharge grease therein, make grease be separated out from the solid powder particle middle reaches of cyperue esculentus
Come.
Further, the mass ratio of protease and cellulase is 3~4:3, or be 1.1~1.3：1；Or be 1.2：
1.Protease can destroy the complexs such as the plant cell wall and degraded lipoprotein of cyperue esculentus；Cellulase being capable of degraded oil Sha
The cell membrane of beans.
Selectable, total addition of protease and cellulase is 3~4wt% of sterile cyperue esculentus powder solution, or
For 3.2~3.8wt%, it is either 3.4~3.6wt% or is 3.5wt%.Wherein, the parameter of enzymolysis processing：Hydrolysis temperature
For 70~75 DEG C, it is either 71~74 DEG C or is 72~73 DEG C, or for 72.5 DEG C；Enzymolysis time is 3~5h, Huo Zhewei
3.5~4.5h, is either 3.7~4.2h or is 3.9~4.1h, or is 4h.Inventor's research is found, when the temperature of enzymolysis
Degree, time, enzyme addition in the above range when, hydrolysis result is optimal, and the dissolution rate of grease is high in cyperue esculentus.
Step S3：Inoculation includes the mixed bacteria of bacillus subtilis and trichoderma reesei in enzymolysis liquid, and in 30~35
Aerobic fermentation is carried out at DEG C 2~3 days, obtain zymotic fluid.
Microbial fermentation processing is carried out to enzymolysis liquid, microorganism carries out metabolism fermentation using the oil-bearing material in zymotic fluid,
Produce a variety of flavor substances, predominantly alcohol, phenol, Ester, the fragrance of cyperue esculentus can be significantly improved, improve its mouthfeel.Its
In, in mixed bacteria, the ratio between unit clump count of bacillus subtilis and trichoderma reesei is 2~3：1, or be 2.5：1.Mixing
The inoculum concentration of strain is the 10~15% of enzymolysis liquid, is either 12~14% or is 13%.
Step S4：After zymotic fluid is handled through demulsification, centrifugation.
The mode of demulsification processing has a variety of, is demulsified using demulsifier, either centrifugation demulsification or stewing process.
In preferred embodiment, preferably centrifugation demulsification.
After zymotic fluid is handled through demulsification, centrifuge again, non-oil component (protein, polysaccharide, carbon dust etc.) separated with grease,
The crude oil of cyperue esculentus is made.Selectable, centrifugal condition is：Rotating speed is 4000~5000rmp, or is 4200~4800rmp,
It is either 4400~4 600rmp or is 4500rmp；Centrifugation time is 20~30min, or is 23~27min, Huo Zhewei
25min。
To sum up, the method that this microbe fermentation method that present embodiment is provided prepares cyperus esculentus oil, first using protease
Cyperue esculentus is handled with cellulase, the cell membrane of cyperue esculentus is destroyed, promotes grease to be discharged from cell；Pass through microorganism again
Fermentation method, is added composite bacteria, it is fermented using the enzymolysis liquid of cyperue esculentus, a variety of flavor substances is produced, by zymotic fluid
Obtained cyperus esculentus oil is centrifuged after demulsification processing.This method is without organic solvent, and technique is simple, without using large scale equipment, and
Grease extraction efficiency is high.
Present embodiment also provides a kind of cyperus esculentus oil product, and it includes cyperus esculentus oil as described above, and bromatology
Upper acceptable additive.The characteristics of cyperue esculentus as made from the above method has aromatic flavour, comfortable taste, and oil is limpid
Transparent, oil quality is good so that using the cyperus esculentus oil product prepared by this cyperus esculentus oil, mouthfeel is good, quality is high.
The feature and performance to the present invention are described in further detail with reference to embodiments：
Embodiment 1
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. 500Kg cyperue esculentus powder is mixed with pH for 7.5 phosphate buffer solution, solid-liquid ratio is 1：10；And in 60
Soaked at DEG C after 2.5h, sterilization treatment, obtain sterile cyperue esculentus powder solution.
B. sterile cyperue esculentus powder solution is mixed with protease, cellulase, total addition of protease and cellulase
For the 4wt% of sterile cyperue esculentus powder solution, and enzymolysis processing is carried out, obtain enzymolysis liquid.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 2：1), inoculum concentration is the 10% of enzymolysis liquid, carries out aerobic fermentation, obtains zymotic fluid.
D. after zymotic fluid is handled through demulsification, centrifugation produces cyperus esculentus oil.
Embodiment 2
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. 500Kg cyperue esculentus powder is mixed with pH for 7.6 phosphate buffer solution, solid-liquid ratio is 1：8；And in 60 DEG C
After lower immersion 2.3h, sterilization treatment, sterile cyperue esculentus powder solution is obtained.
B. sterile cyperue esculentus powder solution is mixed with protease, cellulase, total addition of protease and cellulase
For the 3wt% of sterile cyperue esculentus powder solution, and enzymolysis processing is carried out, obtain enzymolysis liquid.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 2.5：1), inoculum concentration is the 14% of enzymolysis liquid, carries out aerobic fermentation, obtains zymotic fluid.
D. after zymotic fluid is handled through demulsification, centrifugation produces cyperus esculentus oil.
Embodiment 3
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. 500Kg cyperue esculentus powder is mixed with pH for 7.7 phosphate buffer solution, solid-liquid ratio is 1：9；And in 63 DEG C
After lower immersion 2h, sterilization treatment, sterile cyperue esculentus powder solution is obtained.
B. sterile cyperue esculentus powder solution is mixed with protease, cellulase, total addition of protease and cellulase
For the 3.5wt% of sterile cyperue esculentus powder solution, and enzymolysis processing is carried out, obtain enzymolysis liquid.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 3：1), inoculum concentration is the 13% of enzymolysis liquid, carries out aerobic fermentation, obtains zymotic fluid.
D. after zymotic fluid is handled through demulsification, centrifugation produces cyperus esculentus oil.
Embodiment 4
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 20 mesh is mixed with pH for 7.5 phosphate buffer solution, solid-liquid ratio is 1：10；
And in soaking 2.5h at 60 DEG C, the sterilization treatment 20min at 120 DEG C obtains sterile cyperue esculentus powder solution.
B., sterile cyperue esculentus powder solution mixes to (mass ratio of protease and cellulase is with protease, cellulase
1.2:1), total addition of protease and cellulase is the 4wt% of sterile cyperue esculentus powder solution, and is digested at 75 DEG C
3h is handled, enzymolysis liquid is obtained.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 2：1), inoculum concentration is the 10% of enzymolysis liquid, and in progress aerobic fermentation 2 days at 35 DEG C,
Obtain zymotic fluid.
D. after zymotic fluid is handled through demulsification, 30min is centrifuged under rotating speed is 4000rmp, cyperus esculentus oil is produced.
Embodiment 5
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 23 mesh is mixed with pH for 7.6 phosphate buffer solution, solid-liquid ratio is 1：8；And
In soaking 2.3h at 62 DEG C, the sterilization treatment 30min at 110 DEG C obtains sterile cyperue esculentus powder solution.
B., sterile cyperue esculentus powder solution mixes to (mass ratio of protease and cellulase is with protease, cellulase
1：1), total addition of protease and cellulase be sterile cyperue esculentus powder solution 3wt%, and at 74 DEG C carry out enzymolysis at
4.5h is managed, enzymolysis liquid is obtained.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 2.5：1), inoculum concentration is the 14% of enzymolysis liquid, and in progress aerobic fermentation 2 at 30 DEG C
My god, obtain zymotic fluid.
D. after zymotic fluid is handled through demulsification, 23min is centrifuged under rotating speed is 4800rmp, cyperus esculentus oil is produced.
Embodiment 6
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 25 mesh is mixed with pH for 7.7 carbonate buffer solution, solid-liquid ratio is 1：9；And
In soaking 2h at 63 DEG C, the sterilization treatment 25min at 115 DEG C obtains sterile cyperue esculentus powder solution.
B., sterile cyperue esculentus powder solution mixes to (mass ratio of protease and cellulase is with protease, cellulase
4:3), total addition of protease and cellulase is the 3.5wt% of sterile cyperue esculentus powder solution, and is digested at 70 DEG C
5h is handled, enzymolysis liquid is obtained.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 3：1), inoculum concentration is the 13% of enzymolysis liquid, and in progress aerobic fermentation 2.5 at 32.5 DEG C
My god, obtain zymotic fluid.
D. after zymotic fluid is handled through demulsification, 25min is centrifuged under rotating speed is 4500rmp, cyperus esculentus oil is produced.
Embodiment 7
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 27 mesh is mixed with pH for 7.9 carbonate buffer solution, solid-liquid ratio is 1：9；And
In soaking 1.8h at 64 DEG C, the sterilization treatment 28min at 113 DEG C obtains sterile cyperue esculentus powder solution.
B., sterile cyperue esculentus powder solution mixes to (mass ratio of protease and cellulase is with protease, cellulase
1.1:1), total addition of protease and cellulase is the 3.3wt% of sterile cyperue esculentus powder solution, and in carrying out enzyme at 73 DEG C
Solution processing 4h, obtains enzymolysis liquid.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 2.8：1), inoculum concentration is the 12% of enzymolysis liquid, and in progress aerobic fermentation 2.4 at 33 DEG C
My god, obtain zymotic fluid.
D. after zymotic fluid is handled through demulsification, 28min is centrifuged under rotating speed is 4200rmp, cyperus esculentus oil is produced.
Embodiment 8
The present embodiment provides a kind of method that microbe fermentation method prepares cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 30 mesh is mixed with pH for 8.0 phosphate buffer solution, solid-liquid ratio is 1：9；And
In soaking 1.5h at 65 DEG C, the sterilization treatment 23min at 117 DEG C obtains sterile cyperue esculentus powder solution.
B., sterile cyperue esculentus powder solution mixes to (mass ratio of protease and cellulase is with protease, cellulase
1.2:1), total addition of protease and cellulase is the 3.8wt% of sterile cyperue esculentus powder solution, and in carrying out enzyme at 72 DEG C
Solution processing 3.5h, obtains enzymolysis liquid.
C. in enzymolysis liquid inoculation include bacillus subtilis and trichoderma reesei mixed bacteria (bacillus subtilis with it is inner
The ratio between unit clump count of family name's trichoderma is 2.2：1), inoculum concentration is the 15% of enzymolysis liquid, and in progress aerobic fermentation 2.6 at 34 DEG C
My god, obtain zymotic fluid.
D. after zymotic fluid is handled through demulsification, 20min is centrifuged under rotating speed is 5000rmp, cyperus esculentus oil is produced.
Comparative example 1
This comparative example provides a kind of method for preparing cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 20 mesh is mixed with pH for 7.5 phosphate buffer solution, solid-liquid ratio is 1：10；
And in soaking 2.5h at 60 DEG C, the sterilization treatment 20min at 120 DEG C obtains sterile cyperue esculentus powder solution.
B., sterile cyperue esculentus powder solution mixes to (mass ratio of protease and cellulase is with protease, cellulase
1.2:1), total addition of protease and cellulase is the 4wt% of sterile cyperue esculentus powder solution, and is digested at 75 DEG C
3h is handled, enzymolysis liquid is obtained.
C. after zymotic fluid is handled through demulsification, 30min is centrifuged under rotating speed is 4000rmp, cyperus esculentus oil is produced.
Comparative example 2
This comparative example provides a kind of method for preparing cyperus esculentus oil, and it includes：
A. the cyperue esculentus powder (500Kg) of 20 mesh is mixed with pH for 7.5 phosphate buffer solution, solid-liquid ratio is 1：10；
And in soaking 2.5h at 60 DEG C, the sterilization treatment 20min at 120 DEG C obtains sterile cyperue esculentus powder solution.
B. inoculation includes mixed bacteria (the withered grass bud of bacillus subtilis and trichoderma reesei in sterile cyperue esculentus powder solution
The ratio between unit clump count of spore bacillus and trichoderma reesei is 2：1), inoculum concentration is the 10% of enzymolysis liquid, carries out aerobic fermentation, obtains
Zymotic fluid.
C. after zymotic fluid is handled through demulsification, 30min is centrifuged under rotating speed is 4000rmp, cyperus esculentus oil is produced.
Experimental example
Cyperus esculentus oil physicochemical property compares：
The physical and chemical index of cyperue esculentus is determined using the common method of this area：
Acid value presses GB/T5530-2005 animal and plant fats acid value and acidity assaying；
Saponification value presses GB/T5534-1985 grease soap valency determination methods；
Iodine value is determined by GB/T5532-1995 vegetable oil iodine value；
Peroxide value examines determination of POV method to determine by GB/T558-2005 vegetable fat；
Average oil concentration uses soxhlet extraction method.
Every physical and chemical index of detection embodiment 1~8 and comparative example 1~2, and calculate grease extraction as stated above
Rate, as a result as shown in table 1：
The cyperus esculentus oil physicochemical property of table 1. and recovery rate compare
As shown in Table 1, compared with comparative example 1~2, the method for preparing cyperus esculentus oil that the embodiment of the present invention 1~8 is provided
In, the recovery rate of cyperus esculentus oil is higher (74.61~82.34%).Thus illustrate, the method provided using the present invention, grease is carried
Take rate high, the utilization rate of cyperue esculentus is high.
Compared with comparative example 1~2, as the acid number of the cyperus esculentus oil obtained by the embodiment of the present invention 1~8, saponification number and mistake
Oxidation number is relatively low, and iodine number is higher.Thus illustrate, the degree of becoming sour for the cyperus esculentus oil obtained by method that the present invention is provided is low, has
Beneficial to the preservation of cyperue esculentus；Meanwhile, less free fatty, and the peroxide value of the cyperus esculentus oil are contained in the cyperus esculentus oil
Low, freshness is high.In addition, the iodine number of the cyperus esculentus oil is high, illustrate that the degree of unsaturation of the cyperus esculentus oil is high.
In summary, the method that this microbe fermentation method that the present invention is provided prepares cyperus esculentus oil, without organic solvent,
Technique is simple, and without using large scale equipment, and grease extraction efficiency is high.Obtained cyperus esculentus oil, aromatic flavour, comfortable taste,
Oil is as clear as crystal, and oil quality is good.
Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that without departing substantially from the present invention's
Many other changes and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of method that microbe fermentation method prepares cyperus esculentus oil, it is characterised in that it includes：
Cyperue esculentus powder is mixed with cushioning liquid, and in soaking 1.5~2.5h at 60~65 DEG C, carries out sterilization treatment, obtains nothing
Bacterium cyperue esculentus powder solution；
The sterile cyperue esculentus powder solution is mixed with protease, cellulase, and in carrying out enzymolysis processing at 70~75 DEG C, obtained
To enzymolysis liquid；
Inoculation includes the mixed bacteria of bacillus subtilis and trichoderma reesei in the enzymolysis liquid, and in progress at 30~35 DEG C
Aerobic fermentation 2~3 days, obtains zymotic fluid；And
After the zymotic fluid is handled through demulsification, centrifugation.
2. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the protease
Mass ratio with the cellulase is 3~4:3.
3. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the protease
Total addition with the cellulase is 3~4wt% of the sterile cyperue esculentus powder solution.
4. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that at the enzymolysis
The enzymolysis time of reason is 3~5h.
5. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the Mixed Microbes
In kind, the ratio between unit clump count of the bacillus subtilis and the trichoderma reesei is 2~3：1.
6. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the Mixed Microbes
The inoculum concentration planted is the 10~15% of the enzymolysis liquid.
7. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the zymotic fluid
After being handled through demulsification, 20~30min is centrifuged under rotating speed is 4000~5000rmp.
8. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the buffering is molten
The pH value of liquid is 7.5~8.0, and the cushioning liquid includes carbonate buffer solution and/or phosphate buffer solution.
9. the method that microbe fermentation method according to claim 1 prepares cyperus esculentus oil, it is characterised in that the cyperue esculentus
The solid-liquid ratio of powder and the cushioning liquid is 1：8~10.
10. a kind of cyperus esculentus oil product, it is characterised in that it is included obtained by the method for any one as described in claim 1~9
Cyperus esculentus oil, and acceptable additive in bromatology.
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