CN107042125A

CN107042125A - The controller used in syphilis diagnosis device that sample driving can be removed fast with function element

Info

Publication number: CN107042125A
Application number: CN201610092234.2A
Authority: CN
Inventors: 李榕生
Original assignee: Individual
Current assignee: Individual
Priority date: 2016-02-05
Filing date: 2016-02-05
Publication date: 2017-08-15

Abstract

The present invention relates to the controller used in syphilis diagnosis device that a kind of sample driving can be removed fast with function element, belong to analysis testing field.There are some problems with dimethyl silicone polymer i.e. PDMS that is cheap and easily processing to make the substrate of controller used in syphilis diagnosis micro-fluidic chip；Its surface of the PDMS material is strongly hydrophobic, and targetedly surface is modified or surface modification, and its effect is difficult to persistently, and this case aims to solve the problem that the serial relevant issues.This case main points are, the selected PDMS with ecosystem surface of substrate, and the chain ring type magnetic holding device for being equiped with miniature ultrasonic transducer units is socketed is positioned at the test liquid stream terminal of the micro-fluidic chip its neighbor positions, simultaneously, ultrasonic wave damper is installed at the sample introduction end of the micro-fluidic chip, reach ultrasonic intensity rapid decrement in short distance, so as to form interfacial tension difference at the two ends of the chip, thereby facilitate test liquid stream to be flowed along originally hydrophobic capillary channel to the terminal direction.

Description

The controller used in syphilis diagnosis device that sample driving can be removed fast with function element

Technical field

The present invention relates to the controller used in syphilis diagnosis device that a kind of sample driving can be removed fast with function element, belong to analysis test neck Domain.

Background technology

The micro-fluidic controller used in syphilis diagnosis technical background of associated multi-channel, may refer to the applications for a patent for invention such as CN 200910160439.X Case.

Only for the microflow control technique overall general picture of itself, it may refer to famous micro-fluidic expert Mr. Lin Ping Cheng and go out not long ago Monograph " diagram Microfluid based Lab on a chip ", the monograph is published via Science Press, and the monograph is for microflow control technique Past, now, and, in terms of vision of the future etc., suffer from detail, be deep into long discussion of detail.

So, the Important Problems of this case that to have a talk below concern.

The basic framework of micro-fluidic chip, including it is etched with the substrate of small fluid course and the cover plate fit together therewith, Small fluid course on the substrate, assembling upper cover plate before, it is apparent on see to be exactly some micro-channels, wait until thereon After covering cover plate, just real closure forms the small fluid course, and the conduit inner surface of the micro-channel is together with surround this The part cover plate of micro-channel constitutes described small fluid course together；So, it is clear that this after assembling is completed is small Fluid course, the major part of its inner surface area is the inner surface area of that micro-channel, in other words, in the micro-channel The state or property on surface substantially determine the integrality or property of the small fluid course；Therefore say, this is built in base The inner surface state or inner surface property of micro-channel on piece are key factors；In principle, it is any to keep or protecting substantially The material of its solid forms is held, can be used to make substrate and cover plate, such as, the material that can act as substrate and cover plate can be with It is monocrystalline silicon piece, quartz plate, sheet glass, high polymer such as dimethyl silicone polymer, polymethyl methacrylate, makrolon etc. Deng；Certainly, the selection of substrate and the selection of cover plate be able to can also be differed with identical；From material consumption, manufacture difficulty and From the point of view of in terms of application popularization prospect etc., between these materials exist not small difference, the especially selection of that substrate, influence compared with Greatly.

In various substrate making materials, dimethyl silicone polymer, i.e. PDMS, comparatively very easily shaping, so Substrate on make that micro-channel is extremely simple, and the lower cost for material makes substrate with the polydimethyl siloxane material, Micro-channel is being suppressed or etched thereon, and is being engaged with the cover plate that the cheap material such as glass or polypropylene or other plastic sheets makes, It is a kind of more satisfactory selection seemingly；Certainly, patch material can also select to use cheap polydimethyl siloxane material： So, this substrate selection is the scheme of polydimethyl siloxane material, and material is extremely cheap, makes extremely simple, seems and should also be as It is extremely easy to popularize, promotes.

But, thing is really not so simple.

First, this polydimethyl siloxane material, that is, the material that the letter PDMS that abridges is referred to, itself are a kind of strong Hydrophobic material, micro-channel is built on this material, if without being operated for the modified of the micro-channel surface, then, After overall assembling is completed, that is, cover after cover plate, because the micro-channel its inner surface in structure occupies most liquid circulation The inner surface in road, then, its strong hydrophobic property of the PDMS micro-channels inner surface, is deciding factor, it can cause class Be similar to the aqueous solution the fine liquid stream of polar liquid by becoming very difficult, its flow resistance is big, or even general Micropump is all It is difficult to promote, certainly, if cover plate also selects to use the PDMS material, then, problem is substantially identical, similar； Therefore, among prior art, modification is modified particular for the micro-channel inner surface on the PDMS material, is necessary Operation；So, this is pretty troublesome for the modified operation of PDMS micro-channel inner surfacesThat falls nor this problem, structure Perplex into serious technical, be another problem：PDMS polymer molecules tool inside its body phase of this PDMS material substrate There are PDMS polymer molecules inside the characteristic for diffusing to the surface, migrating automatically, this substrate body phase to diffuse to the surface, move automatically The characteristic of shifting, will cause the state after modification by its inner surface of that micro-channel of surface modifying and decorating can not maintain foot Enough long time, the holding time for micro-channel its inner surface state after that is surface-modified is substantially only sufficient to complete in laboratory The time of portion's test experiments needs；In other words, by surface modification or the PDMS micro-channel inner surfaces of surface modification, its The surface state that is formed can not be lasting after modification or after saying modification, but soon automatically tend to or say and become surface again and change Surface state before property, returns to the strong hydrophobic surface state of that script in the shorter time, then, just think, Such micro-fluidic chip can largely make, mass storage, be widely popularized, and answer is it is obvious that is, impossible. Micro-channel on this PDMS material, can not pump similar to the fine liquid stream of polar solvent of the aqueous solution if not doing surface modification Send and pass through, chip also cannot just be used；And if having done surface modification, the state after its modification can not be persistently kept again, also Being equally can not popularization and application.

So, how to accomplish that substrate can either be made using cheap PDMS material, and table in the micro-channel can be released Face decorating state can not persistently, chip can not largely make, largely lay in so that be widely popularized it is such a make this area it is numerous specially The puzzlement that industry personnel are entangled with for a long time, the highly difficult problem that exactly one its obvious technology barrier can not despise.

Be present many year in the highly difficult problem, so far, not yet properly settled.

Second, the PDMS material of non-surface modification, above it is stated that, its surface is strongly hydrophobic, this strong hydrophobic Material surface and also another problem, that is, this strong hydrophobic PDMS surfaces can adsorb large biological molecule, and And, these adsorbed large biological molecules can also further depression further on PDMS surfaces, gradually fall into gradually deep, directly It is trapped in heavy within the body phase of PDMS substrates, in fact, this process is partly also due to inside PDMS material body phase Polymer molecule, which has, to be diffused to the surface, caused by travel motion；Such case, can also be explained from another angle, i.e. Continuously from inside PDMS body phases to its diffusion into the surface, migration those polymer molecules, its result moved, be by Gradually those are involved within the body phase of PDMS substrates by the large biological molecule of adsorption, briefly, these are inhaled Attached large biological molecule is exactly to be swallowed up by PDMS substrate body phases；So, this PDMS substrates body phase swallows up large biological molecule Phenomenon, the influence caused by it necessarily causes the severe deviations of all kinds of test data of experiment for being related to large biological molecule.

As described above, be the problem of PDMS substrates, its not only adsorption large biological molecule, and swallow up large biological molecule, So, as the large biological molecule of experiment test object, its disappearance will not stop because the absorption of surface saturation, but, no It is disconnected adsorbed, also constantly swallowed up.

On PDMS substrates in related experiment test process its body phase constantly swallow up test associated biomolecule macromolecular phenomenon, separately It is to say that one kind, which is explained, there are substantial amounts of Minute pores in PDMS body phases, and associated biomolecule macromolecular is by after adsorption, depression Into these Minute pores, and then swallowed up；However, inventor thinks, those can allow the air point of miniature scale Son squeezes into the Minute pores therebetween, and being not equal to them also can directly allow that the large biological molecule of relative large scale enters, and two Person's difference on yardstick is huge, must not make sweeping generalizations.Explanation is bypassed, in any case, the biology of object is analyzed as dependence test Macromolecular is adsorbed by PDMS substrate micro-channels inner surface, and then is constantly swallowed up by PDMS substrate body phases, and this is known objective The phenomenon of presence.

, can be from containment PDMS surfaces to life in order to prevent this PDMS substrate bodies relative to the effect of swallowing up of large biological molecule The absorption of thing macromolecular is addressed, and method is chemically modified modification aiming at the PDMS material surface, for For PDMS is the situation of substrate material, modification exactly is chemically modified to the surface of described micro-channel part, by changing The micro-channel inner surface of modification is learned, its absorption to large biological molecule can be contained, and then avoid large biological molecule quilt PDMS substrate body phases are swallowed up；But, or that old problem, that is, the chemical modification on PDMS material surface changes Surface state after property can not persistently be kept, the polymer molecule inside the PDMS substrate body phases its diffuse to the surface automatically, The process of migration, soon can become that micro-channel inner surface state being modified by surface chemical modification again script strong and dredge Water and the state of strong adsorption large biological molecule, in other words, no matter how professionals in the field turn from side to side, the PDMS Its micro-channel inner surface of substrate is always rapidly to strong hydrophobic surface state evolution.

So, how can either obtain that PDMS material price is extremely cheap, substrate makes extremely easy benefit, can reach again Growth stage contains absorption process of the PDMS substrate micro-channel inner surfaces to large biological molecule, and then prevents PDMS substrate body phases The effect of swallowing up to large biological molecule so that related chip manufactured goods are able to maintain that a prolonged enough, rational shelf-life, It is exactly a very intractable problem.The problem makes the numerous specialties in this area as another problem addressed above, equally Personnel are entangled with, perplexed for a long time, and the problem is equally the highly difficult problem that its obvious technology barrier can not despise.The hardly possible Also be present many year in topic, so far, also not yet properly settled.

The content of the invention

The technical problems to be solved by the invention are to provide a package solution, solve what is addressed above totally Two problems, also, the solution is used into multichannel micro-fluidic chip field applied to controller used in syphilis diagnosis, formed a kind of new Controller used in syphilis diagnosis Multichannel device.

The present invention solves the technical problem by following scheme, and the device that the program is provided is a kind of sample driving Functional Unit The controller used in syphilis diagnosis device that part can be removed fast, the structure of the device includes multichannel micro-fluidic chip, the knot of the micro-fluidic chip Structure includes installing substrate and cover plate together bonded to each other, and the substrate and cover plate are plate object or tablet, the substrate Contain the channel structure via mould pressing process or etching technics formation towards that face of the cover plate, the substrate also contains and the conduit Structure is connected and pierces the window structure via the formation of mould pressing process, etching technics or simple drilling technology of the substrate, mutually The substrate together and the cover plate is jointly mounted to be built into jointly containing pipeline configuration and the liquid pool structure being attached thereto Micro-fluidic chip, the locations of structures of the pipeline is located at the substrate and cover plate interface zone bonded to each other, its side quilt of the window The cover plate is blocked and opposite side is opened, and the locations of structures of the window is exactly the locations of structures of the liquid pool, and the quantity of the liquid pool is Three, its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, its locations of structures of a remaining liquid pool The terminal that liquid flows in its chip during sample introduction test actual positioned at the micro-fluidic chip, the terminal is located remotely from each other with the sample introduction end, One end of the pipeline via be similarly positioned in the manifold shape turnout of the interface zone bonded to each other respectively with positioned at sample introduction end this two Individual liquid pool UNICOM, the other end of the pipeline and a remaining liquid pool UNICOM for the terminal positioned at the micro-fluidic chip, with And, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electrode and reference electrode, the work electricity Pole is made up of conductive electrode and the gold size sensitive membrane for having embedded syphilis specific antigen being attached on the conductive electrode, should The construction of pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, the working electrode Quantity is four, and the installation position of four working electrodes is located in four laterals respectively, and, this four work Specific antigen in the sensitive membrane structure of its top layer gold size of electrode is that the four kinds of syphilis that can be specifically bound to syphilis antibody resist respectively Original matter, four kinds of antigenic substances are syphilis specific antigen TP0684, TP0453, TP0821 and TP0319 respectively, described Its material of working electrode is gold material or thermal decomposition conducting polymer material, and sheet or silk is presented in its pattern of the working electrode Shape, emphasis is that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is the surface of primary form, the original The surface of raw form its be intended to refer to not by any surface chemical modification or the original of the material of any surface chemical modification The surface of raw form, the structure of the device also includes chain ring type magnetic holding device, and six are had in its structure of the chain ring type magnetic holding device Link, the link is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, its appearance profile of the chain link Hexagonal cyclic structure, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure position, the hexagon In the link of opposite side position, its material is magnetic conductivity material, the remainder four of the hexagonal annular structure to two therein of cyclic structure Its material of individual link is non-magnetic material, and the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, should Magnet exciting coil is wound with a link among two links of magnetic conductivity material, the link for being wound with magnet exciting coil is encouraged at it The pole shoe of a magnetic conductivity material is respectively equiped with the both sides neighbor positions of magnetic coil, two pole shoes are had, two pole shoes The link of another magnetic conductivity material is directed to, attaching fixation is equiped with miniature on its surface of the link of another magnetic conductivity material Ultrasonic transducer, relies on the chain ring type magnetic holding device socket of electromagnetism adhesive strength to be positioned at the micro-fluidic chip its described terminal Close position, and, higher-order of oscillation electric signal transmission cable, one end of the higher-order of oscillation electric signal transmission cable is miniature with this Ultrasonic transducer links together；The chain ring type magnetic holding device facilitates the function that the device is disassembled there is provided one；This is miniature super Its major function of acoustic wave transducer is that in the test of micro-fluidic chip actual sample introduction, the ultrasonic wave launched using it reduces sample Interfacial tension between the inwall of solution and the pipeline, can be compatible, also, utilizes the sample introduction end and the end Difference in the distance between end and the miniature ultrasonic transducer units installation position difference and its ultrasonic intensity experienced, Difference between its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, the micro-fluidic chip two ends it Between interfacial tension difference can form pressure gap between the two ends of the micro-fluidic chip, the pressure gap can drive sample molten Liquid is to the end flow；Its function of the miniature ultrasonic transducer units is also contained in sample including being checked with its ultrasonic wave launched Its absorption on the inner surface of pipeline of some large biological molecules, and then check the substrate of the dimethyl silicone polymer material its body With respect to the effect of swallowing up of the large biological molecule；Its function of the substrate of the dimethyl silicone polymer material includes and cover plate and work electricity Pole and the micro-fluidic chip is together built to electrode and reference electrode, the base of the dimethyl silicone polymer material that is soft and having elasticity Piece its function also includes, with its property to the strong absorption of ultrasonic wave, ultrasonic wave being absorbed strongly, and thereby micro-fluidic at this The chip terminal is to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end；And, ultrasonic wave Damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper be microcellular rubber, Sponge rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, the inner side of each clamping limb Face is relative with the medial surface of another clamping limb, and the medial surface of one of clamping limb is close to the sample introduction end of the micro-fluidic chip, The medial surface of another clamping limb is close to the ultrasonic wave damper, the elastic clip entering the micro-fluidic chip with its gripping strength Sample end and the ultrasonic wave damper are clipped together bonded to each otherly, the ultrasonic wave damper with its material brought to ultrasonic wave energy The strong consumption of amount, is further supported within micro-fluidic chip terminal to the limited short distance between the sample introduction end The rapid decrement of ultrasonic intensity is realized, and thereby further expands described its interfacial tension of sample introduction end and its interface of the terminal Difference between power.

The word of magnet exciting coil one art-recognized meanings of itself are known.

The word of magnetic conductivity material one art-recognized meanings of itself are known；Belong to magnetic conductivity material material such as pure iron, Silicon steel, soft magnetic ferrite etc..

Non magnetic material described in this case refers to the material not responded strongly to externally-applied magnetic field, belongs to the non magnetic material Material such as metallic copper, metallic zinc, ormolu, metallic aluminium, almag etc..

The word art-recognized meanings of itself field in Machine Design that is hinged is known.

The gold size sensitive membrane is to be sufficiently mixed chitosan gold size solution and syphilis specific antigenic solution uniformly, uses point sample instrument Point sample is coated on specified structure position, and forms its drying and forming-film.Syphilis specific antigen in the gold size sensitive membrane is equal The Treponema pallidum antigen marked for horseradish peroxidase or glucose oxidase, the gold size sensitive membrane has been included as fixing above-mentioned each Syphilis specific antigen and introduce complementary medium therein, the complementary medium such as chitosan, cellulose acetate, gelatin Therein a kind of or their mixture.

The pipeline and the lateral and the manifold shape turnout in the microfluidic chip structure, its internal diameter chi It is very little to may each be arbitrarily selected size, still, use the consideration in terms of prepare liquid sample and reduction reagent loss less for trying one's best, The passage of capillary level, the capillary level are preferably selected in the pipeline and the lateral and the manifold shape turnout Passage imply that its internal diameter passage suitable with the internal diameter of the capillary on ordinary meaning.Its inner passage of capillary it is transversal Face shape can be arbitrary shape, the shape of cross section is for example circular, oval, square, rectangle, bar shaped, certainly Can be arbitrarily exist bending it is linear, also, the capillary interior shape with the extension of pipeline, different parts Shape of cross section can also allow to be different shapes.Only for the word of capillary one, its art-recognized meanings is known.

What is be related in structure is the electrode of microsize to electrode and reference electrode, and its electrode shape may each be any choosing Fixed shape, the arbitrarily selected shape such as square piece shape, rectangular patch, strip or round sheet etc..It is described to electricity The art-recognized meanings of pole and the reference electrode vocabulary of itself are known.

It is related to several liquid pools in this case microfluidic chip structure, the liquid pool is the pond shape or scrotiform structure for transitional liquid storage Make, its shape of the inner chamber of each liquid pool may each be arbitrarily selected shape, the cavity shape such as cylindrical empty Cavity-like, square column type cavity-like, oblong cavity shape or spherical hollow space shape etc..The size of the liquid pool can be arbitrarily selected Size, still, in order to use prepare liquid sample and reduction reagent loss less as far as possible, the liquid pool is preferably capable of and capillary The liquid pool of the microminiature of matching.

It is public only for professional of the word of ultrasonic transducer one art-recognized meanings of itself for ultrasonic technology field Know.

Various sizes, variously-shaped ultrasonic transducer are commercially available；Commercially available miniature ultrasonic transducer units its sizes can be with It is small to the magnitude only calculated with millimeter.

Only with regard to miniature ultrasonic transducer units its technique for fixing on general industry application solid body surface itself and Speech, is known general technology for the professional in ultrasonic technology field.This case is not to this expansion superfluous words.

Only with regard to naked PDMS substrates itself micro-channel molding or lithographic technique for, be open-and-shut known technology；Together Sample, the technology of hole-opening is even more known simple technique on naked PDMS substrates.This case is not also to this expansion superfluous words.

The industrial products market of involved its all size of higher-order of oscillation electric signal transmission cable is on sale.

The structure of the device can also include higher-order of oscillation electric signal generator；Its is another for the higher-order of oscillation electric signal transmission cable One end can be connected with the higher-order of oscillation electric signal generator.

The involved higher-order of oscillation electric signal generator technology of itself, for the professional in ultrasonic technology field, be It is simple and known；The higher-order of oscillation electric signal generator can be customized to ultrasonic instrument specialized factory.

The preferred scope of its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units be between 5 milliwatts and 9000 milliwatts it Between；The preferred scope of the frequency of its ultrasonic wave operationally launched of the miniature ultrasonic transducer units be between 100KHz with Between 12MHz.

This case device can further include some annexes, the annex such as multiple tracks electrochemical workstation etc., institute certainly The art-recognized meanings for stating multiple tracks electrochemical workstation are known.Each working electrode for being related in this case microfluidic chip structure and , can be respectively via corresponding special the corresponding interface got lines crossed with the multiple tracks electrochemical workstation to electrode and reference electrode etc. Coupled.It is described that special to get lines crossed be for each the corresponding interface of each electrode and the multiple tracks electrochemical workstation is carried out into phase The private cable being mutually coupled with.The micro-fluidic chip in this case device, its structure can also include micro-valve, the number of the micro-valve Amount is not limited, according to actual needs, and the micro-valve can be installed in any required position installed in the microfluidic chip structure； The word of micro-valve one is for the professional of micro fluidic chip technical field, and the art-recognized meanings of itself are known；The micro-valve The manufacturing technology of itself and the use of technology is also known；The component that the micro-valve is not required.

The diameter of the working electrode can allow to be that any setting is easily installed the suitable diameter used, it is however recommended to Or say preferred its scope of the diameter between 0.1 micron to 2000 microns；The length of the working electrode can permit Permitted to be that any setting is easily installed the length used, it is however recommended to or to say preferred its scope of the length be at 1 micron To between 15000 microns.

The gold size for being installed in the working electrode surface layer by spraying or point sample instrument point sample or the coating of other appropriate process is quick Feel film, its thicknesses of layers can allow be any setting treat sample measuring liquid occur electrical signals response thickness, still, push away Preferred thickness is between 10 nanometers and 200 nanometers in other words for the thickness recommended.

The cover plate in chip structure, its material can allow to be any electrical insulating property material, for example：Polypropylene, glass Glass, polymethyl methacrylate, dimethyl silicone polymer, etc., in order to make smaller size of micro-fluidic chip, such as do Realized into the micro-fluidic chip of only 2.0 centimetres to 3.0 centimetres of super-small of length, and in the extremely short distance to ultrasonic wave Extremely fast decay, can preferably dimethyl silicone polymer be used as cover plate.Certainly, selected on large-sized micro-fluidic chip It is used as the cover plate using dimethyl silicone polymer, is also that this case technical scheme is allowed.

Positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal this is remaining Air line distance between one liquid pool can be arbitrarily selected suitable distance as needed, and still, the preferred value of the distance is Between 3 centimetres and 7 centimetres.

Described its thickness of cover plate and substrate can allow be any setting be easy to assembling thickness, the thickness of recommendation or say preferably Thickness be between 1.0 millimeters and 5.0 millimeters.Less thickness is conducive to saving material.

Described its size of ultrasonic wave damper is not limited, and described its length of ultrasonic wave damper, width, thickness may each be basis Need the arbitrary size of setting.

It is known in field of rubber technology only for the word of microcellular rubber one art-recognized meanings of itself.

The microcellular rubber is commercially available.

It is known in field of rubber technology only for the word of sponge rubber one art-recognized meanings of itself.

The sponge rubber is commercially available.

It is known in technical field of macromolecules only for the word of microporous polyurethane elastomer one art-recognized meanings of itself.

The polyurethane micropore elastomer material is commercially available.

The microcellular rubber preferred microporous silicon rubber or micropore fluorubber.

It is known in field of rubber technology only for the word of micropore silicon rubber one art-recognized meanings of itself.

The microporous silicon Rubber market is on sale.

It is known in field of rubber technology only for the word of micropore fluorubber one art-recognized meanings of itself.

The micropore fluorubber is commercially available.

The word of elastic clip one art-recognized meanings of itself are known.

The elastic clip is commercially available.

The application method of this case micro-fluidic chip：

Proposed first based on this case and the first public New stream driving principle, its application running of this case micro-fluidic chip In, the New stream driving method is determined completely without involving any additional Micropump.

The interfacial tension difference of this case to be formed between micro-fluidic chip two ends caused by the ultrasonic wave, drives liquid Stream flows in the capillary channel of the four-way micro-fluidic chip, using four-way electrochemical analytical instrument respectively to four kinds of syphilis Antibody is detected.

Corresponding four kinds of syphilis antibodies, four kinds of syphilis corresponding with being detected with four kinds of syphilis antibodies are detected with four kinds of Treponema pallidum antigens Antigen, similarly, can Diagnosis of Syphilis；The principle of its foundation is, necessarily antigen, antibody and is deposited in syphilis sufferer body, And mutual Reversible binding, reversible conjugate is formed, therefore, antibody and thereby Diagnosis of Syphilis are detected with antigen, and with antibody Detecting antigen and Diagnosis of Syphilis are removed, diagnostic purpose can be reached.Certainly, its corresponding electrode sensitive decorative layer of different means Technically and differ.

The specific detection of this case micro-fluidic chip is as follows using step：

1st, blood serum sample liquid is added in micro-pipe road, under ultrasonic wave driving, various syphilis antibody molecules are by each passage The syphilis specific antigen capture for the corresponding horseradish peroxidase-labeled that gold size sensitive membrane is embedded on electrode surface.

2nd, the syphilis antibody formation immune complex in the syphilis specific antigen and blood serum sample of horseradish peroxidase-labeled.

3rd, using multi-channel electrochemical analyzer, the electron mediators such as catechol are added, using the above-mentioned reaction of amperometric detection Caused curent change, is derived from the species and content of various analytes.

4th, result is subjected to comprehensive analysis, comprehensive diagnos is carried out to syphilis antibody.

It is an advantage of the invention that its close position relies on the socket of electromagnetism adhesive strength fixed in the terminal of the micro-fluidic chip Position described in chain ring type magnetic holding device, with the chain ring type magnetic holding device one magnetic conductivity link attaching installation miniature ultrasonic Wave transducer, the low-power launched using it, the ultrasonic wave of high-frequency band so that without surface chemical modification or come to the surface The compatibility learned between strong hydrophobic its tube wall of micro-fluidic chip internal pipeline and the test object aqueous solution of modification significantly increases Plus, this is test liquid stream by there is provided a realistic possibility；Meanwhile, using dimethyl silicone polymer substrate its to ultrasound The strong absorbability of ripple, in shorter distance, it is, from the terminal to only several centimeters the sample introduction end In the very short distance of yardstick, reach the rapid decrement of ultrasonic intensity, thereby cause institute at the two ends of the micro-fluidic chip The difference of interfacial tension is stated, and then, utilize the pressure between its two ends for being formed of the difference of the interfacial tension between the two ends Difference, driving test liquid stream flows in the strong hydrophobic capillary channel of such a script to the terminal direction.By this Case liquid stream drive scheme, entirely without any come to the surface must be carried out to the substrate and its internal pipeline of the dimethyl silicone polymer material Modification or chemical modification are learned, the laborious procedures of the surface chemical modification or chemical modification have been altogether dispensed with；And altogether dispense with biography The equipment of Micropump in meaning of uniting etc；On the other hand, the ultrasonic wave of the low-power, high-frequency band, additionally it is possible to contain in sample Absorption of the large biological molecule on literalness naked its inner surface of pipeline of dimethyl silicone polymer substrate, and then contain that this gathers Swallow up effect of its body phase of dimethyl siloxane piece to the large biological molecule；The antigen, antibody and antigen and antibody Reversible binding thing is all the type for belonging to described large biological molecule certainly；Due to described suction-operated and the described work that swallows up With effectively being contained, therefore, dependence test result will be better able to objectively reflect actual conditions；The low-power, height are again and again The effect of section ultrasonic wave, also includes facilitating what the Reversible binding between antigen, antibody react quickly to reach, this causes correlation certainly Test operation can be with than the completion of faster speed.

Due to the surface chemical modification for the dimethyl silicone polymer substrate its relevant surfaces or chemical modification behaviour need not be carried out Make, therefore, this surface chemical modification layer or chemically modified layer not need to exist, then, the dimethyl silicone polymer Its body phase interior polymer molecule of substrate constantly diffuses to the surface automatically, migrate caused by it to surface chemical modification layer or The damaging influence of chemically modified layer is also just not present.

This case its installation position is located at the ultrasonic wave damper at the sample introduction end, from its terminal of this case micro-fluidic chip to Said on the sport technique segment that ultrasonic intensity rapid decrement is realized in the such short distance in sample introduction end, its effect of the ultrasonic wave damper is suitable In the size expansion device of micro-fluidic chip, in other words, the ultrasonic wave damper its function as and significantly extend miniflow Its terminal of chip is controlled the distance between to sample introduction end, is the equal of from several centimetres of extension of script to more than ten centimetres of length Or twenties centimetres of length or longer length, so so that described terminal to the ultrasonic wave between sample introduction end in short distance The sport technique segment of intensity rapid decrement can relatively easily be reached, also, the size expansion device namely the ultrasonic wave damper Demolition, installation are convenient, it is important that it can also be reused.

The technical scheme of this case has dissolved its application related one to dimethyl silicone polymer substrate addressed above totally Row technical barrier.Based on this case scheme, this kind very cheap polydimethyl siloxane material is just possible in the micro-fluidic chip Prepare, production, using etc. field play bigger effect.

Fixation is attached in the chain ring type magnetic holding device one magnetic conductivity link in this case structure and has installed the miniature ultrasonic Transducer, the function that the device is disassembled is facilitated this architecture provides one, in this way, the chain ring type magnetic holding device is led together with one The miniature ultrasonic transducer units attached in magnetic link just easily can be mutually disengaged with the micro-fluidic chip, then, the portion The component that dividing freely to depart from is reused many times in which just can circulate benignly；The architectural feature is conducive to saving making for the device Use cost.

Brief description of the drawings

Fig. 1 is the schematic diagram of this case device embodiment chip part construction, it is shown that the depression angle of this structure Under chip internal pipeline and each electrode and each related liquid pool structural form, do not depict the chain ring type electromagnetic clamp in the figure Tool and the miniature ultrasonic transducer units set up thereon and other annexes.

Fig. 2 be this case device chain ring type magnetic holding device its with outside side view rough under the micro-fluidic chip socket state, The legend and indicate the relative position relation under the socket state between each component.

Fig. 3 is the outside side view under another viewing angle of this case device；Fig. 3 viewing angle and Fig. 2 sight Angle is examined to be mutually perpendicular to.

In figure, 1,2,10 be the different liquid pool of three installation positions respectively, and 3 be manifold shape turnout, 4,7,11,14 It is that installation position is different but four laterals parallel with one another for forming UNICOM's structure in parallel respectively, 5 be to be installed in lateral 4 The working electrode that specific antigenic substance in the interior sensitive membrane structure of its top layer gold size is syphilis specific antigen TP0684,6 It is that specific antigenic substance in the sensitive membrane structure of its top layer gold size being installed in lateral 7 is syphilis specific antigen TP0453 working electrode, 12 be the specific antigen in the sensitive membrane structure of its top layer gold size being installed in lateral 11 Material is syphilis specific antigen TP0821 working electrode, and 13 be that its top layer gold size being installed in lateral 14 is sensitive The working electrode that specific antigenic substance in membrane structure is syphilis specific antigen TP0319,8 be that, to electrode, 9 be reference electricity Pole, 15 be chain ring type magnetic holding device, and 16 be the terminal of the micro-fluidic chip, and 17 be the sample introduction of the micro-fluidic chip End, 18 be the substrate of dimethyl silicone polymer material, and 19 be cover plate, and 20 be miniature ultrasonic transducer units, and 21 be high frequency vibrating Electric signal transmission cable is swung, 22,29 be the link of two magnetic conductivity materials positioned at different structure position respectively, and this two are led The link of magnetic material end-to-end relation each other in the structure shown here, 23,32 be two pole shoes positioned at different structure position respectively, 24th, 26,28,31,34,36 be six pin joints in six diverse locations, 25,27,33,35 difference respectively It is the link of four non magnetic materials in four diverse locations, 30 be magnet exciting coil；The chain ring type electromagnetic clamp in legend Lamps structure is only the legend structure of signal, and the actual chain ring type magnetic holding device structure is not limited to the legend chain ring type electromagnetic clamp and signed an undertaking Structure；Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid stream Flow direction.

The ultrasonic wave damper is depicted without in legend；Also the elastic clip is depicted without in legend.

Embodiment

In this case that Fig. 1, Fig. 2 and Fig. 3 are shown embodiment, the structure of the device includes multichannel micro-fluidic chip, The structure of the micro-fluidic chip includes the substrate 18 and cover plate 19 of installing bonded to each other together, the substrate 18 and cover plate 19 It is plate object or tablet, that face towards the cover plate 19 of the substrate 18 is contained via mould pressing process or etching technics shape Into channel structure, the substrate 18 also containing be connected with the channel structure and pierce the substrate via mould pressing process, etch Technique or the window structure of simple drilling technology formation, substrate 18 bonded to each other being installed together and the common structure of cover plate 19 Built up the micro-fluidic chip containing pipeline configuration and the liquid pool structure being attached thereto, the liquid pool be liquid pool 1, liquid pool 2, Liquid pool 10, the locations of structures of the pipeline is located at the substrate 18 and the cover plate 19 interface zone bonded to each other, the window one Side is blocked by the cover plate 19 and opposite side is opened, and the locations of structures of the window is exactly the liquid pool 1, liquid pool 2, liquid pool 10 Locations of structures, the quantity of the liquid pool is three, and two liquid pools therein are that liquid pool 1 and its locations of structures of liquid pool 2 are micro- positioned at this The sample introduction end 17 of fluidic chip, a remaining liquid pool is that its locations of structures of liquid pool 10 is surveyed positioned at the actual sample introduction of the micro-fluidic chip The terminal 16 that liquid flows in its chip during examination, the terminal 16 is located remotely from each other with the sample introduction end 17, one end of the pipeline via Be similarly positioned in the manifold shape turnout 3 of the interface zone bonded to each other respectively with positioned at the liquid pool 1 at sample introduction end 17 and the liquid pool 2 UNICOMs, the other end of the pipeline and a remaining liquid pool for the terminal 16 positioned at the micro-fluidic chip are liquid pool 10 UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electrode 8 and reference electrode 9, The working electrode is quick by conductive electrode and the gold size for having embedded syphilis specific antigen being attached on the conductive electrode Feel film to constitute, the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is made up of four lateral parallel connections, institute The quantity for stating working electrode is four, and the installation position of four working electrodes is located in four laterals respectively, and this four Individual working electrode is working electrode 5, working electrode 6, working electrode 12, working electrode 13 respectively, and, this four work Specific antigen in the sensitive membrane structure of its top layer gold size of electrode is that the four kinds of syphilis that can be specifically bound to syphilis antibody resist respectively Original matter, four kinds of antigenic substances are syphilis specific antigen TP0684, TP0453, TP0821 and TP0319 respectively, specifically For ground expansion, 5 be that specific antigenic substance in the sensitive membrane structure of its top layer gold size being installed in lateral 4 is syphilis Specific antigen TP0684 working electrode, 6 be the spy in the sensitive membrane structure of its top layer gold size being installed in lateral 7 Specific Antigen material is syphilis specific antigen TP0453 working electrode, and 12 be its top layer being installed in lateral 11 The working electrode that specific antigenic substance in gold size sensitivity membrane structure is syphilis specific antigen TP0821,13 be to be installed in point The work that the specific antigenic substance in the sensitive membrane structure of its top layer gold size in branch pipe(tube) 14 is syphilis specific antigen TP0319 Electrode, the working electrode 5, working electrode 6, working electrode 12, working electrode 13 its material are gold material or heat point Solve conducting polymer material, the working electrode 5, working electrode 6, working electrode 12, its pattern presentation of working electrode 13 Sheet is thread, and emphasis is, its material of the substrate 18 is dimethyl silicone polymer material, and its surface of substrate 18 is primary The surface of form, the surface of the primary form its be intended to refer to by any surface chemical modification or any surface chemistry not change Property the material primary form surface, the structure of the device also includes chain ring type magnetic holding device 15, the chain ring type electromagnetic clamp Have six links 22,25,27,29,33,35 in 15 its structure of tool, the link 22,25,27,29,33, 35 in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, the hexagonal ring of its appearance profile of the chain link Shape structure, each drift angle of the hexagonal annular structure is that drift angle 24,26,28,31,34,36 its locations of structures is hinge Locations of structures is connect, links of two therein of the hexagonal annular structure in opposite side position are that link 22,29 its material is to lead Magnetic material, the remaining four processes of the hexagonal annular structure be link 25,27,33,35, its material be non magnetic Material, the non magnetic material refers to the material not responded strongly to externally-applied magnetic field, and two links of the magnetic conductivity material are A link among link 22,29 is that magnet exciting coil 30 is wound with link 29, and this is wound with the ring of magnet exciting coil 30 Section 29 is respectively equiped with the pole shoe of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil 30, has described in two Pole shoe, the pole shoe is pole shoe 23 and pole shoe 32, and two pole shoes are that pole shoe 23 and pole shoe 32 are directed to another magnetic conduction Property material link 22, fixation is attached on its surface of the link 22 of another magnetic conductivity material and is equiped with miniature ultrasonic transducing Device 20, relies on the chain ring type magnetic holding device 15 socket of electromagnetism adhesive strength to be positioned at the micro-fluidic chip its described terminal 16 Close position, and, higher-order of oscillation electric signal transmission cable 21, one end of the higher-order of oscillation electric signal transmission cable 21 with The miniature ultrasonic transducer units 20 link together；The chain ring type magnetic holding device 15 facilitates what the device was disassembled there is provided one Function；Its major function of the miniature ultrasonic transducer units 20 is, in the actual sample introduction test of micro-fluidic chip, to be launched using it Ultrasonic wave reduce the interfacial tension between sample solution and the inwall of the pipeline, can be compatible, also, utilize institute State the distance between sample introduction end 17 and the terminal 16 and the installation position of miniature ultrasonic transducer units 20 difference and its institute The difference on ultrasonic intensity experienced, its interfacial tension of sample introduction end 17 described in induced synthesis and described its interface of terminal 16 Difference between tension force, micro-fluidic chip two ends are that the interfacial tension difference between sample introduction end 17 and terminal 16 can be at this The two ends of micro-fluidic chip are to form pressure gap between sample introduction end 17 and terminal 16, and the pressure gap can drive sample molten Liquid flows to the direction of terminal 16；Its function of the miniature ultrasonic transducer units 20 also includes holding back with its ultrasonic wave launched Only its absorption on the inner surface of pipeline of large biological molecule contained in sample, and then check the dimethyl silicone polymer material Swallow up effect of its body phase of the substrate 18 of matter to the large biological molecule；Its work(of the substrate 18 of the dimethyl silicone polymer material It can include and cover plate 19 and working electrode 5, working electrode 6, working electrode 12, working electrode 13 and to electrode 8 and reference Electrode 9 together builds the micro-fluidic chip, soft and have the substrate 18 of the dimethyl silicone polymer material of elasticity its function also Including with its property to the strong absorption of ultrasonic wave, being absorbed strongly to ultrasonic wave, and thereby in the micro-fluidic chip terminal 16 to the rapid decrement that ultrasonic intensity is realized within the limited short distance between the sample introduction end 17；And, ultrasonic wave abatement Device, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；Its material of the ultrasonic wave damper is microcellular rubber, sponge Rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, the medial surface of each clamping limb with The medial surface of another clamping limb is relative, and the medial surface of one of clamping limb is close to the sample introduction end 17 of the micro-fluidic chip, The medial surface of another clamping limb is close to the ultrasonic wave damper, the elastic clip entering the micro-fluidic chip with its gripping strength Sample end 17 and the ultrasonic wave damper are clipped together bonded to each otherly, the ultrasonic wave damper with its material brought to ultrasound The strong consumption of wave energy, is further supported limited between micro-fluidic chip terminal 16 to the sample introduction end 17 Short distance within realize the rapid decrement of ultrasonic intensity, and thereby further expand described its interfacial tension of sample introduction end 17 with Difference between described its interfacial tension of terminal 16.

It is depicted without being also depicted without the elastic clip in the ultrasonic wave damper, legend in legend.

Arrow in legend indicate the micro-fluidic chip its in actual motion, by pressure at two ends difference drive, its test liquid The flow direction of stream.

The chain ring type magnetic holding device 15 is depicted without in Fig. 1 and fixed installing is attached in one magnetic conductivity material link The miniature ultrasonic transducer units 20 and higher-order of oscillation electric signal transmission cable 21；Also, in Fig. 1, Fig. 2 and Fig. 3 It is depicted without the associate members such as the higher-order of oscillation electric signal generator and multiple tracks electrochemical workstation.

Involved magnetic conductive material and involved nonmagnetic substance market are on sale.

Involved miniature ultrasonic transducer units 20 are commercially available；It can also be customized to ultrasonic transducer producer.

Involved higher-order of oscillation electric signal transmission cable 21 is commercially available；Can also be to ultrasonic transducer producer or cable special manufacturer Family's customization.

Involved higher-order of oscillation electric signal generator market has the product close to needs commercially available；It can also be customized to relevant manufacturers.

Each working electrode in this example structure and electrode and reference electrode via each special cable or can be said respectively Get lines crossed respectively with the corresponding cable interface of the multiple tracks electrochemical workstation as annex or saying interface connection of getting lines crossed.

This case device is that a variety of corresponding syphilis antibodies are detected with a variety of Treponema pallidum antigens, thereby Diagnosis of Syphilis.

Capillary on usual expression and significance refers to hydrophilic capillary glass tube；Capillary channel involved by this case is hydrophobic Capillary form pipeline.

Claims

1. the controller used in syphilis diagnosis device that sample driving can be removed fast with function element, the structure of the device includes multichannel micro-fluidic Chip, the structure of the micro-fluidic chip includes the substrate and cover plate of installing bonded to each other together, and the substrate and cover plate are plate Contain the conduit knot via mould pressing process or etching technics formation in shape thing or tablet, that face towards the cover plate of the substrate Structure, the substrate also containing be connected with the channel structure and pierce the substrate via mould pressing process, etching technics or simply punch The window structure of technique formation, substrate bonded to each other being installed together and the cover plate be built into jointly containing pipeline configuration with And the micro-fluidic chip for the liquid pool structure being attached thereto, the locations of structures of the pipeline is positioned at the substrate and cover plate boundary bonded to each other Face region, its side of the window is blocked by the cover plate and opposite side is opened, and the locations of structures of the window is exactly the structure of the liquid pool Position, the quantity of the liquid pool is three, and its locations of structures of two liquid pools therein is located at the sample introduction end of the micro-fluidic chip, remaining Under liquid pool its locations of structures terminal that liquid flows in its chip when being located at the actual sample introduction test of the micro-fluidic chip, the end End is located remotely from each other with the sample introduction end, and one end of the pipeline is via the manifold shape turnout point for being similarly positioned in the interface zone bonded to each other Not with the Liang Ge liquid pools UNICOM positioned at sample introduction end, this of the terminal of the other end of the pipeline with being located at the micro-fluidic chip is remaining Under a liquid pool UNICOM, and, the working electrode that is sequentially respectively installed in the pipeline on diverse location and to electrode and Reference electrode, the working electrode is by conductive electrode and is attached on the conductive electrode and has embedded syphilis specific antibody Gold size sensitive membrane constitute, the construction of the pipeline is in parallel construction, and the pipeline in parallel construction is by four lateral parallel connections Constitute, the quantity of the working electrode is four, the installation position of four working electrodes is located at four laterals respectively It is interior, and, the specific antigen in the sensitive membrane structure of its top layer gold size of four working electrodes is respectively can be special to syphilis antibody Property combine four kinds of Treponema pallidum antigen materials, four kinds of antigenic substances are syphilis specific antigen TP0684, TP0453, TP0821 respectively And TP0319, its material of the working electrode is gold material or thermal decomposition conducting polymer material, its pattern of the working electrode Sheet or thread is presented, it is characterised in that its material of the substrate is dimethyl silicone polymer material, and its surface of the substrate is primary The surface of form, the surface of the primary form its be intended to refer to by any surface chemical modification or any surface chemistry not change Property the material primary form surface, the structure of the device also includes chain ring type magnetic holding device, the chain ring type magnetic holding device its Have six links in structure, the link is in strip, shaft-like or bar-shaped, and each adjacent link is hinged to form chain link, should The hexagonal cyclic structure of its appearance profile of chain link, its locations of structures of each drift angle of the hexagonal annular structure is articulated structure Position, in the link of opposite side position, its material is magnetic conductivity material, the hexagonal rings to two therein of the hexagonal annular structure Its material of the remaining four processes of shape structure is non-magnetic material, and the non magnetic material refers to not strong to externally-applied magnetic field Magnet exciting coil is wound with a link among the material of response, two links of the magnetic conductivity material, this is wound with excitation wire The link of circle is respectively equiped with the pole shoe of a magnetic conductivity material in the both sides neighbor positions of its magnet exciting coil, has two poles Boots, two pole shoes are directed to attach on the link of another magnetic conductivity material, its surface of the link of another magnetic conductivity material Fixed to be equiped with miniature ultrasonic transducer units, relying on the chain ring type magnetic holding device socket of electromagnetism adhesive strength, to be positioned at this micro-fluidic The close position of its terminal of chip, and, higher-order of oscillation electric signal transmission cable, the higher-order of oscillation electric signal transmission cable One end linked together with the miniature ultrasonic transducer units；The chain ring type magnetic holding device facilitates what the device was disassembled there is provided one Function；Its major function of the miniature ultrasonic transducer units is, in the actual sample introduction test of micro-fluidic chip, to utilize surpassing that it is launched Sound wave reduces the interfacial tension between sample solution and the inwall of the pipeline, can be compatible, also, enters described in The distance between sample end and the terminal and the miniature ultrasonic transducer units installation position difference and its ultrasound experienced Difference between difference in intensity of wave, its interfacial tension of sample introduction end described in induced synthesis and the terminal its interfacial tension, this is micro- Interfacial tension difference between the fluidic chip two ends can form pressure gap between the two ends of the micro-fluidic chip, the pressure Difference can drive sample solution to the end flow；Its function of the miniature ultrasonic transducer units also includes the ultrasound launched with it Ripple checks in sample contained large biological molecule its absorption on the inner surface of pipeline, and then checks the polydimethylsiloxanes Swallow up effect of its body phase of the substrate of alkane material to the large biological molecule；Its function bag of the substrate of the dimethyl silicone polymer material Include with cover plate and working electrode and the micro-fluidic chip is together built to electrode and reference electrode, the poly- diformazan that is soft and having elasticity The substrate of radical siloxane material its function also includes, with its property to the strong absorption of ultrasonic wave, ultrasonic wave being absorbed strongly, And thereby realize the quick of ultrasonic intensity within micro-fluidic chip terminal to the limited short distance between the sample introduction end Successively decrease；And, ultrasonic wave damper, its profile of ultrasonic wave damper is in block, tabular or bar-shaped；The ultrasonic wave damper Its material is microcellular rubber, sponge rubber or microporous polyurethane elastomer；And, elastic clip, the elastic force accompanies two clamping limbs, The medial surface of each clamping limb is relative with the medial surface of another clamping limb, and the medial surface of one of clamping limb is close to the miniflow The sample introduction end of chip is controlled, the medial surface of another clamping limb is close to the ultrasonic wave damper, and the elastic clip will with its gripping strength The sample introduction end of the micro-fluidic chip and the ultrasonic wave damper are clipped together bonded to each otherly, and the ultrasonic wave damper is with its material institute The strong consumption to ultrasonic energy brought, is further supported in the micro-fluidic chip terminal between the sample introduction end The rapid decrement of ultrasonic intensity is realized within limited short distance, and thereby further expand described its interfacial tension of sample introduction end with Difference between its interfacial tension of the terminal.

2. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In the pipeline and the lateral and the manifold shape turnout are capillary channel.

3. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In, the thermal decomposition conducting polymer be by polyimides or polyacrylonitrile be heat-treated through anoxybiotic after the conductive material that is formed.

4. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In, the width or diameter of the working electrode between 0.1 micron to 2000 microns, and, the length of the working electrode Degree is between 1 micron to 15000 microns.

5. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In the thickness of the gold size sensitive membrane is between 10 nanometers and 200 nanometers.

6. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In its material of the cover plate in structure is dimethyl silicone polymer material.

7. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In, positioned at the sample introduction end of the micro-fluidic chip two liquid pools its with positioned at the micro-fluidic chip terminal this remaining one Air line distance between individual liquid pool is between 3 centimetres and 7 centimetres；The cover plate in structure and substrate its thickness are between 1.0 Between millimeter and 5.0 millimeters.

8. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In the microcellular rubber is micropore silicon rubber or micropore fluorubber.

9. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists Also include higher-order of oscillation electric signal generator in the structure of, the device, its other end of the higher-order of oscillation electric signal transmission cable with The higher-order of oscillation electric signal generator is connected.

10. the controller used in syphilis diagnosis device that sample driving according to claim 1 can be removed fast with function element, its feature exists In its specified ultrasonic wave transmission power of the miniature ultrasonic transducer units is between 5 milliwatts and 9000 milliwatts, the miniature ultrasonic The frequency of its ultrasonic wave operationally launched of wave transducer is between 100KHz and 12MHz.