CN107028846B - Cosmetic containing blue embryo matrix and its preparation method - Google Patents
Cosmetic containing blue embryo matrix and its preparation method Download PDFInfo
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- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
A cosmetic containing orchid embryo matrix comprises at least one cosmetic raw material and at least one orchid embryo extract. Adding the orchid embryo extract into the cosmetic raw material to prepare a cosmetic composition. The orchid embryo extract has high expression gene group, and can produce beneficial components for human skin.
Description
Technical Field
The invention relates to a cosmetic containing blue embryo matrix; in particular to a cosmetic added with a mature blue embryo functional gene.
Background
COSMETIC COMPOSITIONs CONTAINING ORCHID EXTRACT, such as "COSMETIC COMPOSITION CONTAINING ORCHID EXTRACT", AND COSMETIC COMPOSITION CONTAINING ORCHID EXTRACT, as disclosed in U.S. patent application publication No. 20110002968, are disclosed.
Another prior art cosmetic containing orchid extract, such as "Use of a branched mineral extract as an active agent to present or delay" patent application of signs of skin aging "of U.S. patent application publication No. 20090258093, discloses an orchid extract as an activator for preventing or delaying skin aging.
Another prior art cosmetic containing Orchid Extract, such as the invention patent application of "Use of an Extract of the organic flower culture as a Skin moisturizing Agent" in U.S. patent application publication No. 20090238848, discloses a cosmetic containing Orchid Extract as a Skin moisturizing Agent.
Another prior cosmetic containing orchid extract, such as the invention patent application of "Brassocattleya marcella koss ordered extract and use of skin degradation" of U.S. patent application publication No. 20090232758, discloses the use of orchid extract as a skin depigmentation agent.
However, the aforementioned U.S. patent application publication nos. 20110002968, 20090258093, 20090238848 and 20090232758 disclose only orchid extracts as various skin care treatments, and do not disclose how to use orchid-based materials for skin care. Therefore, there is a need for further products with skin caring effect. The foregoing patent applications are merely illustrative of the state of the art to which this invention pertains and are not intended to limit the scope of the invention.
In view of the above, the present invention provides a blue embryo matrix-containing cosmetic and a method for manufacturing the same, wherein a blue embryo matrix is added into a cosmetic raw material, and the blue embryo matrix comprises at least one mature blue embryo functional gene, so as to solve the problem of poor skin care effect of the existing cosmetic.
Disclosure of Invention
The invention mainly aims to provide a cosmetic added with a blue embryo matrix and a manufacturing method thereof, wherein the blue embryo matrix is added into a cosmetic raw material, and the blue embryo matrix comprises at least one mature blue embryo functional gene which is taken from a mature blue embryo so as to achieve the purpose of improving the skin care effect.
In order to achieve the above object, the cosmetic composition with added blue embryo matrix of the present invention comprises:
at least one cosmetic raw material having a first predetermined content; and
at least one blue embryo matrix with a second predetermined content, adding the blue embryo matrix into the cosmetic raw material to prepare a cosmetic composition;
wherein the orchid embryo matrix comprises at least one mature orchid embryo functional gene which can produce ingredients beneficial to human skin.
The orchid embryo matrix of the preferred embodiment of the invention is taken from an orchid embryo.
The cosmetic material of the preferred embodiment of the present invention is selected from a cosmetic material, a make-up material or a perfume material to be made into a cosmetic, a make-up product or a perfume.
The cosmetic material according to the preferred embodiment of the present invention is selected from a powdery material, a pasty material or a liquid material.
The embryonic substrate of the preferred embodiment of the present invention is derived from phalaenopsis.
The mature blue embryo functional genes of the preferred embodiment of the present invention comprise a cell division and replication expression gene group, a cell response and feedback expression gene group, a cell and metabolism regulation expression gene group or other functional expression gene groups.
The cell division and replication expression gene group of the preferred embodiment of the present invention includes RNA processing and modification functions, chromosome structure and dynamics functions, cell cycle control, division and chromosome allocation functions, translation, ribosome structure, biosynthesis functions, transcription functions, replication, recombination and repair functions, cell wall/membrane/inner membrane biosynthesis functions, post-translational modification, protein transformation, molecular chaperone functions and nuclear structure functions and cytoskeleton functions.
The cellular response and feedback expression gene group of the preferred embodiment of the present invention includes cellular motor function, message transmission mechanism function, defense mechanism function and extracellular structure function.
The cell and metabolic regulation expression gene group according to the preferred embodiment of the present invention includes energy generation and conversion functions, amino acid transport and metabolism functions, nucleotide transport and metabolism functions, carbohydrate transport and metabolism functions, coenzyme transport and metabolism functions, lipid transport and metabolism functions, inorganic ion transport and metabolism functions, secondary metabolite biosynthesis, transport and metabolism functions, and intracellular movement, secretion, and vesicle transport functions.
In order to achieve the above object, the method for producing a cosmetic containing a blue base according to the present invention comprises:
carrying out artificial pollination on a orchid by a pollen block;
selecting a mature fruit pod from the orchid;
subjecting the mature fruit pod to a liquid culture, a solid culture or both to obtain an orchid embryo;
drying the orchid embryo to obtain a dried orchid embryo;
pulverizing the dried orchid embryo to obtain an orchid embryo fragment or powder;
extracting the orchid embryo fragment to obtain a orchid embryo matrix, wherein the orchid embryo matrix comprises at least one mature orchid embryo functional gene; and
adding the orchid embryo matrix into the cosmetic raw material to obtain a cosmetic composition.
The drying process of the preferred embodiment of the present invention is a freeze drying process to obtain a freeze-dried orchid embryo.
The pulverization process of the preferred embodiment of the present invention is a low temperature pulverization process or a low temperature oscillation pulverization process.
The extraction process of the preferred embodiment of the present invention is a solvent extraction process.
The invention has the beneficial effects that:
the invention provides a cosmetic added with a blue embryo matrix and a manufacturing method thereof, wherein the blue embryo matrix is added into a cosmetic raw material, and the blue embryo matrix comprises at least one mature blue embryo functional gene which is taken from a mature blue embryo, so as to achieve the purpose of improving the skin care effect.
Drawings
FIG. 1 is a flow chart of the method for manufacturing a cosmetic product with an added blue embryo matrix according to the preferred embodiment of the present invention.
FIG. 2 is a diagram showing the relationship between mature blue embryo expression genes and the number of genes in a cosmetic composition according to a preferred embodiment of the present invention.
FIG. 3 is a graph of the concentration of blue embryo matrix in a cosmetic composition of the present invention as a function of DPPH radical scavenging activity.
FIG. 4 is a graph showing the comparison of iron ion chelating ability of a control group with a blue germ matrix extract and a blue germ matrix powder in a cosmetic according to a preferred embodiment of the present invention.
Detailed Description
In order that the invention may be fully understood, there shall now be described by way of illustration preferred embodiments in conjunction with the accompanying drawings, without limiting the scope of the invention.
In the description of the preferred embodiments of the present invention, the term "orchid embryo matrix" is used, and the terms related to the aforementioned "orchid embryo matrix" include "orchid embryo matrix," "orchid embryo matrix," and "orchid embryo matrix" or other similar terms (e.g., orchid embryo stem cell matrix), but the scope of the present invention is not limited thereto.
In general, cosmetics comprise a range of products that can be broadly divided into: cosmetic, color cosmetic and perfume. For example, the cosmetic product comprises: lotions, creams; the color cosmetic comprises: lipstick, eye shadow, blush, pressed powder; the perfume comprises: essences, perfumes, colognes, but they are not intended to limit the scope of the present invention.
In terms of product use functionality, cosmetic definition categories include: hair cosmetics, hair lotions, cosmetic oils, perfumes, powders, cream emulsions, bath cosmetics, face washes, foundations, lip creams, skin care cosmetics, eye cosmetics, nail cosmetics, and soaps, but the scope of the present invention is not limited thereto.
The cosmetics added with the blue embryo matrix according to the preferred embodiment of the present invention can be applied to various general cosmetics including cosmetics, color cosmetics and perfumes, but are not intended to limit the scope of the present invention. In addition, the blue embryo base-containing cosmetic according to the preferred embodiment of the present invention is suitable for absorption or adsorption on human skin, but is not intended to limit the scope of the present invention.
The present description uses the definition of "Adsorption" that the cosmetic ingredients stay only on the surface of the stratum corneum; "Absorption" is defined as the penetration of a portion of the cosmetic ingredients through the stratum corneum into the deep layers of the epidermis, dermis and even subcutaneous tissue. For example, the ingredients of the cosmetic composition of the preferred embodiment of the present invention added with orchid embryo matrix can be absorbed through the sweat gland mouth, hair follicle mouth or through the keratinocytes or the interstitial spaces of the keratinocytes.
FIG. 1 is a flow chart of the method for manufacturing a cosmetic with a blue embryo matrix according to the preferred embodiment of the present invention. Referring to fig. 1, the method for manufacturing a cosmetic with a blue embryo matrix according to a preferred embodiment of the present invention includes step S1: first, a orchid is artificially pollinated in a pollen lump for a predetermined time (e.g., about 150 days or 5 months) by using a tool (e.g., tweezers or other special tools) so as to perform a subsequent series of processing operations (steps S2 to S7).
Referring to fig. 1 again, the method for manufacturing a cosmetic with a blue blank matrix according to a preferred embodiment of the invention includes step S2: next, a mature pod (ripe pod) is selected from the orchid in a suitable manner for a subsequent series of processing operations (steps S3-S7). For example, the orchid of the preferred embodiment of the present invention is obtained from phalaenopsis or related strains.
Referring to fig. 1 again, the method for manufacturing a cosmetic with a blue blank matrix according to a preferred embodiment of the invention includes step S3: then, the mature fruit pod is subjected to a liquid culture, a solid culture or other culture (e.g., plate culture) for a predetermined time (e.g., about 6 hours) to obtain an orchid embryo (orchid embryo) for a subsequent series of processing (steps S4-S7).
For example, the solid culture of the preferred embodiment of the present invention is to place the plant tissue on a solid medium (e.g., MS growth medium), usually selected in a glass or plastic bottle. Further, the plate culture is to bury the plant tissue in a solid medium (e.g., MS growth medium), and to select the culture in a petri dish. In addition, liquid culture is to immerse or float the plant tissue selection in or on a liquid medium (e.g., MS growth medium), and includes stationary culture, suspension culture and floating culture.
For example, the preferred embodiment of the present invention selects using growth points or test tube seedling leaves to induce Proliferation of Spheroids (PLBs) in minute bodies (micropropagation), but it is not intended to limit the scope of the present invention; alternatively, mass production of tissue-cultured seedlings by culturing axillary buds of growing points or pedicel nodes is selected, but the scope of the present invention is not limited thereto.
Referring to fig. 1 again, the method for manufacturing a cosmetic with a blue blank matrix according to a preferred embodiment of the invention includes step S4: next, the orchid embryo is dried for a predetermined time (e.g. about 48 hours or 2 days) by a suitable drying device to obtain a dried orchid embryo for a subsequent series of processing (steps S5-S7).
For example, the drying process of the preferred embodiment of the present invention is a vacuum drying process, but it is not intended to limit the scope of the present invention. The vacuum drying method is not required to be rapidly frozen, but directly enters a vacuum chamber for drying. For example, freeze-drying is the best low-temperature drying method at present, and is also the best way to preserve the components of the material from being damaged during the drying process. In a preferred embodiment of the present invention, a single or multiple freeze-drying process (e.g., about-60 ℃) is performed using suitable freeze-drying equipment to remove water from the orchid embryos and obtain the dried orchid embryos.
Referring to fig. 1 again, the method for manufacturing a cosmetic with a blue blank matrix according to a preferred embodiment of the invention includes step S5: next, the dried orchid embryo is pulverized for a predetermined time (e.g. about 10 seconds to 30 seconds) by a suitable pulverizing device to obtain a orchid embryo fragment or powder for subsequent processing (steps S6 and S7). For example, the pulverization process of the preferred embodiment of the present invention is a cryogenic pulverization process or a cryogenic oscillation pulverization process (e.g., selecting an oscillation frequency of about 30/sec).
FIG. 2 is a diagram showing the relationship between mature blue embryo expression genes and the number of genes in the cosmetic composition according to the preferred embodiment of the present invention. Referring to fig. 1 and 2, the method for manufacturing a cosmetic with a blue embryo matrix according to a preferred embodiment of the present invention comprises steps S6: the orchid embryo fragment is then extracted by an extraction device to obtain a orchid embryo matrix for subsequent processing (step S7). In addition, the blue embryo matrix comprises at least one mature blue embryo functional gene, and is prepared into blue embryo matrix powder by a freeze drying mode.
Referring to FIG. 2, for example, the mature blue embryo functional genes include a cell division and replication expression gene group, a cell response and feedback expression gene group, a cell and metabolism regulation expression gene group or other functional expression gene groups, and the expression gene number of the expression gene group is mostly over 500, and some of them are as high as 1000 or 1500, even over 4000.
For example, the cell division and replication expression gene group includes RNA (ribonucleic acid) processing and modification (processing and modification) functions, chromosome structure and dynamics (chromosome structure and dynamics) functions, cell cycle control, division and chromosome allocation (cell cycle control, cell division, chromosome replication) functions, translation, ribosome structure, biosynthesis (translation, ribosome structure and biosynthesis) functions, transcription (transcription) functions, replication, recombination and repair (replication and repair) functions, cell wall/cell membrane/inner membrane biosynthesis (cell wall/cell/expression biosynthesis) functions, post-translational modification, protein transformation, molecular chaperone (pore) modification, cell structure and cell nucleus functions. The cell response and feedback expression gene group comprises a cell motility (cell motility) function, a signal transduction mechanisms (signal transduction mechanisms) function, a defense mechanism (defense mechanisms) function and an extracellular structure (extracellular structures) function. The cell and metabolic regulation expression gene group includes energy production and conversion (energy production and conversion) function, amino acid transport and metabolism (amino acid transport and metabolism) function, nucleotide transport and metabolism (nucleotide transport and metabolism) function, carbohydrate transport and metabolism (carbohydrate transport and metabolism) function, coenzyme transport and metabolism (coenzyme transport and metabolism) function, lipid transport and metabolism (lipid transport and metabolism) function, inorganic ion transport and metabolism (inorganic ion transport and metabolism) function, secondary metabolite biosynthesis, transport and metabolism (secondary metabolite biosynthesis and metabolism) function, and intracellular movement, secretion, transport and metabolism (intracellular transport and metabolism) function.
For example, the extraction process of the preferred embodiment of the present invention is a solvent extraction process, such as: 70% to 95% alcohol (MeOH) was chosen for extraction. In addition, other solvent extraction methods select other solvents (such as benzene) to extract the volatile and nonvolatile matrix components in the plant; subsequently, the mixture after extraction is filtered again with a solvent to obtain an extract.
Referring to fig. 1 again, the method for manufacturing a cosmetic with a blue blank matrix according to a preferred embodiment of the invention includes step S7: finally, a cosmetic raw material is properly selected, and the orchid-embryo matrix is added into the cosmetic raw material to prepare a cosmetic composition. For example, the cosmetic material of the preferred embodiment of the present invention is selected from a cosmetic material, a make-up material, a perfume material, a powder material, a paste material, or a liquid material.
Regarding the effective activity (antioxidant capacity or DPPH free radical scavenging capacity) of the blue embryo matrix, functional analysis experiments are carried out by successful university orchid research and development centers, and the experimental results show that the blue embryo matrix has the DPPH free radical scavenging capacity at various concentrations. FIG. 3 is a graph showing the relationship between different concentrations of blue embryo base and DPPH radical scavenging ability of cosmetic compositions according to preferred embodiments of the present invention. As shown in FIG. 3, the antioxidant capacity at the treatment concentration of 50 μ g/ml was statistically significantly different from that of the control group consisting of DPPH and pure methanol, and at the treatment concentration of 300 μ g/ml, the scavenging capacity of DPPH radicals was more than 20%.
In general, the pro-oxidative action of metal ions, among which Fe is the major factor responsible for lipid peroxidation, is often the major factor2+Often the most influential pro-oxidants, which promote lipid oxidation. Thus, use is made of Fe2+Color reaction of the complex with ferrozine, and determination of blue-embryo substrate to Fe2+Chelating ability of ions. The skin collagen protein decomposition causes the skin elasticity loss and wrinkle generation, and the activity of the collagen enzyme can be detected by using the change of the light absorption value caused by the decomposition of the synthetic peptide substrate by the collagenase.
Fig. 4 is a schematic diagram showing the comparison of iron ion chelating ability between the blue-germ matrix extract and blue-germ matrix powder and a control group (Quercetin) in the cosmetic according to the preferred embodiment of the present invention. Referring to fig. 4, the results of the experiment show that the blue embryo matrix crude extract indeed has iron ion chelating ability (anti-oxidation, skin-shielding) and collagen degradation inhibition (collagen loss prevention, anti-wrinkle ability).
The invention can select an encapsulation technology which is used for coating the blue-germ matrix powder, so as to form an encapsulated blue-germ matrix, and the encapsulated blue-germ matrix is added into the cosmetic raw material. Microcapsules are tiny containers, also called microspheres, that use a wall membrane (outer membrane) to enclose tiny powders of the orchid embryo matrix, which can be chosen to be 1mm to 1 μm in size. The microcapsule completely isolates the embedding substance from the external environment so as to reduce the reaction of the embedded blue-embryo matrix powder and the external environment. In use, the encapsulated blue germ matrix can be dissolved to release the blue germ matrix powder material using appropriate conditions of use.
For example, the present invention may alternatively employ encapsulation materials comprising colloids, starches, modified starches, celluloses, lipids, proteins or inorganic substances. Coatings used for encapsulation even in the food industry may be referred to as edible films, which range in kind from water-soluble colloids, lipids and colloid/lipid complexes. The edible films can be used interchangeably in practice to form multiple layers of the encapsulated blue embryo matrix.
For example, water-soluble colloids include proteins, cellulose (cellulose derivatives), alginates (alginates), starches, chitosan, and other polysaccharides; lipids include waxes, acylglycerols (acylglycerols), and fatty acids. For example, the present invention employs water-soluble colloids comprising gum Arabic (gum acacia), beta-cyclodextrin (cyclodextrin), and chitosan (chitosan).
The foregoing description of the preferred embodiments is merely exemplary of the invention and its technical features, and the techniques of the embodiments may be suitably modified and/or substituted in various substantially equivalent manners; therefore, the scope of the claims of the present invention should be determined by the scope of the appended claims.
Claims (9)
1. A cosmetic product supplemented with a blue germ base, comprising:
at least one cosmetic raw material having a first predetermined content; and
at least one blue embryo matrix with a second predetermined content, the blue embryo matrix is taken from a mature fruit pod, and the blue embryo matrix is added into the cosmetic raw material to prepare a cosmetic composition;
wherein the orchid embryo matrix comprises at least one mature orchid embryo functional gene which can produce ingredients beneficial to human skin, and the orchid embryo matrix is prepared by the following steps:
carrying out artificial pollination on a orchid by a pollen block;
selecting a mature fruit pod from the orchid;
subjecting the mature fruit pod to a liquid culture or a solid culture on an MS growth medium for 6 hours to obtain an orchid embryo;
drying the orchid embryo to obtain a dried orchid embryo;
pulverizing the dried orchid embryo to obtain an orchid embryo fragment or powder;
subjecting the fragment of orchid embryo to solvent extraction treatment with 70% to 95% alcohol to obtain a orchid embryo matrix.
2. The blue embryo matrix-containing cosmetic of claim 1, wherein the blue embryo matrix is obtained from a blue flower embryo.
3. The cosmetic with added orchid base as claimed in claim 1, wherein the cosmetic material is selected from a cosmetic material, a make-up material, a perfume material, a powder material, a paste material or a liquid material.
4. The cosmetic with added orchid embryo matrix of claim 1, wherein the orchid embryo matrix is obtained from Phalaenopsis.
5. The blue embryo matrix-added cosmetic of claim 1, wherein the mature blue embryo functional genes comprise a cell division and replication expression gene group, a cell response and feedback expression gene group, a cell and metabolic regulation expression gene group or other functional expression gene groups.
6. The blue embryo matrix-added cosmetic according to claim 5, wherein the cell division and replication expression gene group comprises RNA processing and modification functions, chromosome structure and dynamics functions, cell cycle control, division and chromosome allocation functions, translation, ribosome structure, biosynthesis functions, transcription functions, replication, recombination and repair functions, cell wall/cell membrane/inner membrane biosynthesis functions, post-translational modification, protein transformation, chaperone functions and nuclear structure functions and cytoskeleton functions; the cell response and feedback expression gene group comprises a cell movement function, an information transmission mechanism function, a defense mechanism function and an extracellular structure function; the cell and metabolism regulation expression gene group comprises energy generation and conversion functions, amino acid transportation and metabolism functions, nucleotide transportation and metabolism functions, carbohydrate transportation and metabolism functions, coenzyme transportation and metabolism functions, lipid transportation and metabolism functions, inorganic ion transportation and metabolism functions, secondary metabolite biosynthesis, transportation and metabolism functions, intracellular movement, secretion and vesicle transportation functions.
7. A method for producing a cosmetic containing a blue base, comprising:
carrying out artificial pollination on a orchid by a pollen block;
selecting a mature fruit pod from the orchid;
subjecting the mature fruit pod to a liquid culture or a solid culture on an MS growth medium for 6 hours to obtain an orchid embryo;
drying the orchid embryo to obtain a dried orchid embryo;
pulverizing the dried orchid embryo to obtain an orchid embryo fragment or powder;
extracting the fragment of the orchid embryo by using 70-95% alcohol to obtain a orchid embryo matrix, wherein the orchid embryo matrix is taken from a mature fruit pod and comprises at least one mature orchid embryo functional gene; and
adding the orchid embryo matrix into the cosmetic raw material to obtain a cosmetic composition.
8. The method of claim 7, wherein the drying process is a freeze-drying process to obtain a lyophilized orchid embryo.
9. The method for producing a cosmetic containing a blue germ matrix according to claim 7, wherein the pulverization treatment is a low-temperature pulverization treatment or a low-temperature shaking pulverization treatment.
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| TWI701038B (en) * | 2018-12-07 | 2020-08-11 | 嘉藥學校財團法人嘉南藥理大學 | Orchid bud embryo tissue extract that can activate energy, anti-wrinkle, anti-inflammatory and whitening |
| AU2020439080A1 (en) * | 2020-04-01 | 2021-12-09 | Chiahua Liang | Energy-activating, anti-wrinkle, anti-inflammatory, and whitening orchid embryo tissue extract |
Citations (5)
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|---|---|---|---|---|
| CN1777410A (en) * | 2003-04-24 | 2006-05-24 | 科蒂股份有限公司 | Cosmetic for depigmentation |
| CN1995371A (en) * | 2006-12-19 | 2007-07-11 | 王书刚 | Embryo cell active protein preparation method and its uses in skin care product |
| CN101268759A (en) * | 2008-04-16 | 2008-09-24 | 中国林业科学研究院林业研究所 | Cultivation method for Dendrobium initial species embryo |
| TW201420128A (en) * | 2012-11-28 | 2014-06-01 | Taiwan Orchid Professionals Co Ltd | Extract of Phalaenopsis amabilis embryo, and the preparation process and uses thereof |
| CN104814904A (en) * | 2015-05-18 | 2015-08-05 | 樊文花 | Phalaenopsis brightening mask and preparation method thereof |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1777410A (en) * | 2003-04-24 | 2006-05-24 | 科蒂股份有限公司 | Cosmetic for depigmentation |
| CN1995371A (en) * | 2006-12-19 | 2007-07-11 | 王书刚 | Embryo cell active protein preparation method and its uses in skin care product |
| CN101268759A (en) * | 2008-04-16 | 2008-09-24 | 中国林业科学研究院林业研究所 | Cultivation method for Dendrobium initial species embryo |
| TW201420128A (en) * | 2012-11-28 | 2014-06-01 | Taiwan Orchid Professionals Co Ltd | Extract of Phalaenopsis amabilis embryo, and the preparation process and uses thereof |
| CN104814904A (en) * | 2015-05-18 | 2015-08-05 | 樊文花 | Phalaenopsis brightening mask and preparation method thereof |
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