CN107018706A - A kind of method for improving Caragana Microphylla Seed sprouting and seedling fast-growth - Google Patents
A kind of method for improving Caragana Microphylla Seed sprouting and seedling fast-growth Download PDFInfo
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- CN107018706A CN107018706A CN201710413019.2A CN201710413019A CN107018706A CN 107018706 A CN107018706 A CN 107018706A CN 201710413019 A CN201710413019 A CN 201710413019A CN 107018706 A CN107018706 A CN 107018706A
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- caragana microphylla
- seed
- germanium
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- microphylla seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Abstract
The invention discloses a kind of method for improving Caragana Microphylla Seed sprouting and seedling fast-growth, including：Seed treatment：Caragana Microphylla Seed after sterilization is placed in concentration to soak 20~25h under conditions of 22~28 DEG C in 10~30 μm of ol/L germanium-containing solution；And seed is sprouted：In humidity it is that 45~55%, illumination is that 500~2000lx, temperature are to be placed in culture box under conditions of 23~26 DEG C to cultivate by the Caragana Microphylla Seed after immersion, daily light application time is 7.5 8.5h, and remaining time is light culture.Raising Caragana Microphylla Seed provided by the present invention sprouts and the method for seedling fast-growth is simple and easy to apply, low-cost, simple to operate.Percentage of seedgermination after processing is significantly improved, growth of seedling is accelerated.
Description
Technical field
The present invention relates to vegetable seeds Cultivating techniques field, and in particular to one kind improves Caragana Microphylla Seed sprouting and seedling is quick
The method of growth.
Background technology
Caragana microphylla (Caragana Korshinskii Kom) is the perennial big shrub of fallen leaves of pulse family Caragana, main distribution
On the Inner Mongol of NW China, Shaanxi, Ningxia, Gansu and other places.Root has root nodule, drought-resistant barren, is the Northwest's afforestation and plant
It is resumed main Native species.The being rich in nutrition material of caragana microphylla branch, leaf, flower, fruit, seed, is good forage grass and feed.Branch
Containing grease, dry and wet is not avoided in burning, is good fire wood.But Caragana Microphylla Seed sprouts and seedling growth stage has some and is stranded
Difficulty, such as germination rate are relatively low, sprout irregular, and growth of seedling is slow etc., have fettered nursery and the direct seeding forestation effect of caragana microphylla.Cause
This, improves the germination rate of Caragana Microphylla Seed, accelerates the growth of seedling, has weight for the success of Analyse on Forestation with Korshinsk Peashrub under northwest drought environment
The meaning wanted.
The content of the invention
In order to solve the problem of prior art is present, improve Caragana Microphylla Seed it is an object of the invention to provide one kind and sprout and children
The method of seedling fast-growth, it can improve the germination rate and seedling growth rate of Caragana Microphylla Seed.
The technical scheme that the present invention solves above-mentioned technical problem is as follows：
A kind of method for improving Caragana Microphylla Seed sprouting and seedling fast-growth, including：
Seed treatment：Caragana Microphylla Seed after sterilization is placed in the germanium-containing solution that concentration is 10~30 μm of ol/L 22
20~25h is soaked under conditions of~28 DEG C；And
Seed is sprouted：In humidity it is that 45~55%, illumination is that 500~2000lx, temperature are by the Caragana Microphylla Seed after immersion
It is placed in culture box and cultivates under conditions of 23~26 DEG C, daily light application time is 7.5-8.5h, remaining time is light culture.
Caragana Microphylla Seed is placed in germanium-containing solution by the present invention to be soaked, and is had using germanium and is removed the biology such as free radical, anti-aging
Activity improves the Antioxidant Enzyme Systems of Interior Seed, and removes free radical excessive in seed, so as to promote low vitality kind
The sprouting and growth of son.Meanwhile, the present invention is by the Caragana Microphylla Seed after germanium-containing solution is handled in suitable humidity, illumination and temperature
Cultivated under the conditions of degree, by suitable cultivating seeds environment, further improve the germination rate of seed and the growth speed of seedling
Rate.The present invention is simple and easy to apply, low-cost, simple to operate, and it is bright to improve the effect of seed germination rate and seedling growth rate
It is aobvious.
Moreover, the present invention is directed to Caragana Microphylla Seed to germanium-containing solution sensitivity, determines and be specially adapted for Caragana Microphylla Seed
μm ol/L of the concentration of germanium-containing solution, i.e., 10~30.In the concentration range, both effectively free radical can be removed using germanium ion
Characteristic, thus promote Caragana Microphylla Seed sprout and grow, the toxicity of high concentration germanium-containing solution can be avoided again and to Caragana Microphylla Seed
Sprout the harm for producing inhibitory action and being brought to environment.
The above-mentioned technical proposal of the present invention can also be improved further：
In the preferable implementation of the present invention, above-mentioned seed treatment step, which is additionally included in, is placed in Caragana Microphylla Seed in germanium-containing solution
The ultrasound treatment step carried out before immersion, the ultrasound treatment step includes：By after Caragana Microphylla Seed gauze wrapped 5~10
Ultrasonically treated 15~30min is carried out under conditions of DEG C, the frequency of ultrasonic wave is 40~60kHz.
The present invention can change the permeability of seed plasma membrane by ultrasonically treated, help suction of the seed cell to germanium ion
Receive, so as to improve the effect that germanium ion removes free radical.Ultrasonic wave changes the sky that permeability of cell membrane is mainly based upon ultrasonic wave
Change is acted on.When ultrasonic wave is propagated in a liquid, vibrations of the medium molecule centered on its equilbrium position will be caused, in ultrasonic wave
Compress in phase, intermolecular distance reduces；And in sparse phase, intermolecular distance will increase.In the suitable feelings of ultrasonic intensity
Under condition, reversible membrane permeability can be caused to change.Cell itself can repair the breakage of wall and film, thus this recoverable damage
Wound can change the permeability of cytoplasma membrane, promote the exchange of intraor extracellular material, so as to promote the sprouting of seed.The present invention is by lemon
Bar seed carries out ultrasonically treated in the case where frequency is 40~60kHz, can effectively change seed cell wall membrane structure, help germanium from
Son ensures that cell envelope structure change has invertibity while entering intracellular.Meanwhile, under conditions of temperature is 5~10 DEG C
Progress is ultrasonically treated, and cytoactive can be reduced to a certain extent by low temperature, it is to avoid cell is too early by itself reparation
Close the interchange channel on cell envelope, so as to influence the follow-up absorption to germanium ion.
In the preferable implementation of the present invention, in above-mentioned seed treatment step, concretely comprising the following steps for Caragana Microphylla Seed is sterilized：Will
Caragana Microphylla Seed is cleaned 2~3 times with volumetric concentration for 50~70% alcoholic solution, and 5~6min is cleaned every time, then dense with quality
The liquor natrii hypochloritis spent for 6~8% cleans 3~4 times, cleans 4~5min every time, then with distilled water flushing 4~5 times, every time
Clean 2~3min.
The present invention carries out 2~3 times short first with alcoholic solution when being carried out disinfection to Caragana Microphylla Seed to Caragana Microphylla Seed
Time disinfects, it is to avoid because directly causing the problem of Seed viability is low to the damage caused by seed embryo with alcohol-pickled,
Then supplement sterilization is carried out using sodium hypochlorite, to overcome the alcohol short time to sterilize halfway hidden danger, will finally sterilized thorough
Caragana Microphylla Seed distilled water flushing 4~5 times, the alcohol and sodium hypochlorite that remain in the surface of the seed are cleaned up.This hair
It is bright to be carried out several times when being carried out disinfection using different antiseptic solutions to seed, and disinfecting time is all controlled several every time
Within minute, this have the advantage that, it can both fully ensure that sterilization is thorough, antiseptic solution (alcohol can be greatly reduced again
And sodium hypochlorite) to seed belt come negative effect, so as to improve the germination rate and seedling growth rate of seed.
In the preferable implementation of the present invention, the concentration of above-mentioned germanium-containing solution is 10 μm of ol/L.
In the preferable implementation of the present invention, the concentration of above-mentioned germanium-containing solution is 30 μm of ol/L.
In the preferable implementation of the present invention, above-mentioned germanium-containing solution is inorganic germanium solution or organic germanium solution.Wherein, organic germanium
Can be three-fold coordination, four-coordination or without coordination germanium compound.Inorganic Ge can be the oxide of germanium or the chloride of germanium.
In the preferable implementation of the present invention, above-mentioned inorganic germanium solution is germanium dioxide solution.
In the preferable implementation of the present invention, said organogermanium solution is Ge-132 solution.β-hydroxyl second
Base germanium sesquioxide solution is also known as Ge-123.
In the preferable implementation of the present invention, in above-mentioned seed treatment step, the temperature of immersion Caragana Microphylla Seed is 25 DEG C.
In the preferable implementation of the present invention, in above-mentioned seed sprouts step, the humidity of culture Caragana Microphylla Seed is 50%, light
According to being 25 DEG C for 1000lx, temperature.
The invention has the advantages that：
Raising Caragana Microphylla Seed provided by the present invention sprouts and the method for seedling fast-growth is simple and easy to apply, low-cost,
It is simple to operate.Percentage of seedgermination after processing is significantly improved, growth of seedling is accelerated.The method is applied to production, can be carried
The vigor of high Caragana Microphylla Seed, increases the recycling of Caragana Microphylla Seed of low quality, meanwhile, quickly emerge and ensure that under natural conditions
Field seed quick-speed germination seedling, promotes the success of afforestation.The technology has weight for the sprouting and afforestation for promoting Caragana Microphylla Seed
Want meaning.
Brief description of the drawings
Fig. 1 schemes for the contrast experiment of the germination percentage of embodiment 1, embodiment 3 and comparative example 1；
Fig. 2 schemes for the contrast experiment of the seedling length of embodiment 1, embodiment 3 and comparative example 1.
Embodiment
The principle and feature of the present invention are described below in conjunction with accompanying drawing, the given examples are served only to explain the present invention, and
It is non-to be used to limit the scope of the present invention.Unreceipted actual conditions person in embodiment, the bar advised according to normal condition or manufacturer
Part is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional products that can be obtained by commercially available purchase.
The Caragana Microphylla Seed that the present embodiment is used is the local Caragana Microphylla Seed of Shaanxi Province's Yulin City.The Caragana Microphylla Seed of collection is entered
After row impurity elimination, cleaning and selection processing, in indoor storage 2 years, the following example for the present invention.
Embodiment 1：
The method that the present embodiment improves Caragana Microphylla Seed sprouting and seedling fast-growth, comprises the following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed is cleaned 3 times with volumetric concentration for 50% alcoholic solution, 6min is cleaned every time, so
Clean 4 times for 6% liquor natrii hypochloritis with mass concentration afterwards, clean 4min every time, then with distilled water flushing 4 times, every time clearly
Wash 3min.
Germanium-containing solution soaks：Caragana Microphylla Seed after disinfecting is placed in germanium-containing solution of the concentration for 10 μm of ol/L
24h is soaked under conditions of 25 DEG C.Germanium-containing solution is organic germanium solution Ge-123 in the present embodiment.
2. seed is sprouted
In humidity it is that 50%, illumination is that 1000lx, temperature are to be placed in training under conditions of 25 DEG C by the Caragana Microphylla Seed after immersion
Support in box and cultivate, daily light application time is 8h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
Embodiment 2
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 1 of seedling fast-growth is,
The organic germanium solution Ge-123 of embodiment concentration is 20 μm of ol/L, other conditions all same.
Embodiment 3
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 1 of seedling fast-growth is,
The organic germanium solution Ge-123 of embodiment concentration is 30 μm of ol/L, other conditions all same.
Embodiment 4
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 1 of seedling fast-growth is,
The germanium-containing solution of embodiment is germanium dioxide solution, other conditions all same.
Embodiment 5
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 1 of seedling fast-growth is,
The germanium-containing solution of embodiment is germanium dioxide solution and concentration is 20 μm of ol/L, other conditions all same.
Embodiment 6
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 1 of seedling fast-growth is,
The germanium-containing solution of embodiment is germanium dioxide solution and concentration is 30 μm of ol/L, other conditions all same.
Embodiment 7
The method that the present embodiment improves Caragana Microphylla Seed sprouting and seedling fast-growth, comprises the following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed is cleaned 3 times with volumetric concentration for 60% alcoholic solution, 5.5min is cleaned every time,
Then cleaned 4 times for 7.5% liquor natrii hypochloritis with mass concentration, clean 4.5min every time, then with distilled water flushing 5 times,
2.5min is cleaned every time.
It is ultrasonically treated：By Caragana Microphylla Seed with carrying out ultrasonically treated 30min, ultrasonic wave after gauze wrapped under conditions of 5 DEG C
Frequency be 60kHz.
Germanium-containing solution soaks：Caragana Microphylla Seed after ultrasonically treated is placed in germanium-containing solution of the concentration for 10 μm of ol/L
25h is soaked under conditions of 22 DEG C.Germanium-containing solution is organic germanium solution Ge-123 in the present embodiment.
2. seed is sprouted
In humidity it is that 45%, illumination is that 500lx, temperature are to be placed in culture under conditions of 23 DEG C by the Caragana Microphylla Seed after immersion
Cultivated in box, daily light application time is 7.5h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
Embodiment 8
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 7 of seedling fast-growth is,
The germanium-containing solution of embodiment is germanium dioxide solution, other conditions all same.
Embodiment 9：
The method that the present embodiment improves Caragana Microphylla Seed sprouting and seedling fast-growth, comprises the following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed is cleaned 3 times with volumetric concentration for 55% alcoholic solution, 5.5min is cleaned every time,
Then cleaned 3 times for 8% liquor natrii hypochloritis with mass concentration, clean 5min every time, then with distilled water flushing 5 times, every time
Clean 3min.
It is ultrasonically treated：By Caragana Microphylla Seed with carrying out ultrasonically treated 25min, ultrasonic wave after gauze wrapped under conditions of 6 DEG C
Frequency be 50kHz.
Germanium-containing solution soaks：Caragana Microphylla Seed after ultrasonically treated is placed in germanium-containing solution of the concentration for 20 μm of ol/L
22h is soaked under conditions of 28 DEG C.Germanium-containing solution is organic germanium solution Ge-123 in the present embodiment.
2. seed is sprouted
In humidity it is that 50%, illumination is that 1000lx, temperature are to be placed in training under conditions of 25 DEG C by the Caragana Microphylla Seed after immersion
Support in box and cultivate, daily light application time is 8h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
Embodiment 10
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 9 of seedling fast-growth is,
The germanium-containing solution of embodiment is germanium dioxide solution, other conditions all same.
Embodiment 11：
The method that the present embodiment improves Caragana Microphylla Seed sprouting and seedling fast-growth, comprises the following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed is cleaned 2 times with volumetric concentration for 70% alcoholic solution, 6min is cleaned every time, so
Clean 3 times for 8% liquor natrii hypochloritis with mass concentration afterwards, clean 5min every time, then with distilled water flushing 5 times, every time clearly
Wash 3min.
It is ultrasonically treated：By Caragana Microphylla Seed with carrying out ultrasonically treated 20min, ultrasonic wave after gauze wrapped under conditions of 8 DEG C
Frequency be 50kHz.
Germanium-containing solution soaks：Caragana Microphylla Seed after ultrasonically treated is placed in germanium-containing solution of the concentration for 30 μm of ol/L
24h is soaked under conditions of 25 DEG C.Germanium-containing solution is organic germanium solution Ge-123 in the present embodiment.
2. seed is sprouted
In humidity it is that 55%, illumination is that 1500lx, temperature are to be placed in training under conditions of 26 DEG C by the Caragana Microphylla Seed after immersion
Support in box and cultivate, daily light application time is 8.5h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
Embodiment 12
The raising Caragana Microphylla Seed of the present embodiment is sprouted and the difference of method and embodiment 11 of seedling fast-growth is,
The germanium-containing solution of embodiment is germanium dioxide solution, other conditions all same.
Comparative example 1
This comparative example is substantially the same manner as Example 1, and difference is, Caragana Microphylla Seed is soaked in deionized water, specific bag
Include following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed is cleaned 3 times with volumetric concentration for 50% alcoholic solution, 6min is cleaned every time, so
Clean 4 times for 6% liquor natrii hypochloritis with mass concentration afterwards, clean 4min every time, then with distilled water flushing 4 times, every time clearly
Wash 3min.
Deionized water is soaked：Caragana Microphylla Seed after disinfecting is placed in into deionized water to soak under conditions of 25 DEG C
24h。
2. seed is sprouted
In humidity it is that 50%, illumination is that 1000lx, temperature are to be placed in training under conditions of 25 DEG C by the Caragana Microphylla Seed after immersion
Support in box and cultivate, daily light application time is 8h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
Comparative example 2
The difference of this comparative example and embodiment 1 is that organic germanium solution Ge-123 concentration is 8 μm of ol/L, other conditions phase
Together.
Comparative example 3
The difference of this comparative example and embodiment 1 is that organic germanium solution Ge-123 concentration is 32 μm of ol/L, other conditions
It is identical.
Comparative example 4
The difference of this comparative example and embodiment 1 is that germanium-containing solution is germanium dioxide solution and concentration is 8 μm of ol/L, its
His condition all same.
Comparative example 5
The difference of this comparative example and embodiment 1 is that germanium-containing solution is germanium dioxide solution and concentration is 32 μm of ol/L,
Other conditions all same.
Comparative example 6
This comparative example is substantially the same manner as Example 7, and difference is, this comparative example is not carried out at ultrasound to Caragana Microphylla Seed
Reason, specifically includes following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed is cleaned 3 times with volumetric concentration for 60% alcoholic solution, 5.5min is cleaned every time,
Then cleaned 4 times for 7.5% liquor natrii hypochloritis with mass concentration, clean 4.5min every time, then with distilled water flushing 5 times,
2.5min is cleaned every time.
Germanium-containing solution soaks：Caragana Microphylla Seed after disinfecting is placed in germanium-containing solution of the concentration for 10 μm of ol/L
25h is soaked under conditions of 22 DEG C.Germanium-containing solution is organic germanium solution Ge-123 in the present embodiment.
2. seed is sprouted
In humidity it is that 45%, illumination is that 500lx, temperature are to be placed in culture under conditions of 23 DEG C by the Caragana Microphylla Seed after immersion
Cultivated in box, daily light application time is 7.5h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
Comparative example 7
This comparative example is substantially the same manner as Example 7, and difference is, disinfection way is different, and remaining condition is identical, specifically includes
Following steps：
1. seed treatment
Disinfect：Caragana Microphylla Seed volumetric concentration is soaked into 34.5min (with embodiment 7 for 60% alcoholic solution
Sterilize total time identical), then clean with distilled water 12.5min (identical with the cleaning total time of embodiment 7).
It is ultrasonically treated：By Caragana Microphylla Seed with carrying out ultrasonically treated 30min, ultrasonic wave after gauze wrapped under conditions of 5 DEG C
Frequency be 60kHz.
Germanium-containing solution soaks：Caragana Microphylla Seed after ultrasonically treated is placed in germanium-containing solution of the concentration for 10 μm of ol/L
25h is soaked under conditions of 22 DEG C.Germanium-containing solution is organic germanium solution Ge-123 in the present embodiment.
2. seed is sprouted
In humidity it is that 45%, illumination is that 500lx, temperature are to be placed in culture under conditions of 23 DEG C by the Caragana Microphylla Seed after immersion
Cultivated in box, daily light application time is 7.5h, remaining time is light culture.
Wherein, the size of culture box is：Length × width × height=24 × 12 × 12cm, culture cassette bottom portion is covered with three metafiltration paper,
Pour permeable, 300 Caragana Microphylla Seeds are cultivated in each culture box.
The bud ratio and seedling length of above-described embodiment and the Caragana Microphylla Seed of comparative example are determined, measurement result is shown in Table 1.Germination
Rate calculation formula is (seed number of germination)/100 × 100%；A length of of seedling and bud plus and it is long, in terms of centimetre (cm).Table 1
Middle seedling length is taken as taking 10 plants to determine and then average at random.
Table 1
As it can be seen from table 1 the seed cultivated using 1-12 of the embodiment of the present invention methods provided, no matter in germination percentage
Or seedling length, it is above comparative example 1-7, specifically：
(1) it is ultrasonically treated to seed progress in the method provided by 1-6 of the embodiment of the present invention, therefore compared to
Ultrasonically treated embodiment 7-12 is carried out, the former germination percentage and the equal increasing degree of seedling length are less than the latter.Illustrate with germanic
By ultrasonically treated seed can be promoted further to germinate before solution immersion Caragana Microphylla Seed, improve percentage of seedgermination and seedling length
Degree.Also, comparative example 6 is compared with embodiment 7, also further prove ultrasonication to germination and the rush of growth of seedling
Enter effect.
(2) embodiment of the present invention 1 is compared with the comparative example 1 that seed is soaked using deionized water, as a result of germanic molten
Immersion steeps seed, therefore its germination percentage for showing and seedling length are far above comparative example 1.Illustrate that germanium-containing solution can be carried substantially
High percentage of seedgermination and seedling length.
(3) to employ Ge-123 solution or germanium dioxide of the concentration outside 10-30 μm of ol/L scope molten by comparative example 2-5
Liquid, it is compared with comparative example 1, and change is little, and part comparative example does not promote seed germination not only, or even shows to suppress
Effect.Illustrate that the concentration of germanium-containing solution is also very crucial to Caragana Microphylla Seed rudiment.When concentration is less than 10 μm of ol/L, for example, contrast
What example 2 and 4, its germination percentage change without, and seedling length is also slightly reduced, and causes such phenomenon, it may be possible to because
Germanium ion concentration is too low, removes the DeGrain of free radical, so as to influence seed to sprout the growth with seedling.When concentration is less than
During 30 μm of ol/L, such as comparative example 3 and 5, its germination percentage and seedling length are no more than the comparative example 1 handled with deionized water, table
Reveal obvious inhibitory action, cause such phenomenon, it may be possible to because germanium ion concentration is too high, toxicity is shown, so as to give
Seed is sprouted and the growth of seedling is adversely affected.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc. should be included in the scope of the protection.
Claims (10)
1. a kind of method for improving Caragana Microphylla Seed sprouting and seedling fast-growth, it is characterised in that including：
Seed treatment：Caragana Microphylla Seed after sterilization is placed in the germanium-containing solution that concentration is 10~30 μm of ol/L 22~28
20~25h is soaked under conditions of DEG C；And
Seed is sprouted：By the Caragana Microphylla Seed after immersion humidity be 45~55%, illumination be 500~2000lx, temperature be 23~
It is placed in culture box and cultivates under conditions of 26 DEG C, daily light application time is 7.5-8.5h, remaining time is light culture.
2. the method according to claim 1 for improving Caragana Microphylla Seed sprouting and seedling fast-growth, it is characterised in that described
Seed treatment step is additionally included in the ultrasound treatment step for being placed in Caragana Microphylla Seed and being carried out before being soaked in germanium-containing solution, described super
Sonication step includes：By Caragana Microphylla Seed with carrying out ultrasonically treated 15~30min after gauze wrapped under conditions of 5~10 DEG C,
The frequency of ultrasonic wave is 40~60kHz.
3. the method according to claim 2 for improving Caragana Microphylla Seed sprouting and seedling fast-growth, it is characterised in that in institute
State in seed treatment step, sterilize concretely comprising the following steps for Caragana Microphylla Seed：By the wine that Caragana Microphylla Seed volumetric concentration is 50~70%
Smart solution is cleaned 2~3 times, and 5~6min is cleaned every time, then cleans 3~4 with mass concentration for 6~8% liquor natrii hypochloritis
It is secondary, 4~5min is cleaned every time, then with distilled water flushing 4~5 times, 2~3min is cleaned every time.
4. the method for improving Caragana Microphylla Seed sprouting and seedling fast-growth according to any one of claim 1-3, it is special
Levy and be, the concentration of the germanium-containing solution is 10 μm of ol/L.
5. the method for improving Caragana Microphylla Seed sprouting and seedling fast-growth according to any one of claim 1-3, it is special
Levy and be, the concentration of the germanium-containing solution is 30 μm of ol/L.
6. the method for improving Caragana Microphylla Seed sprouting and seedling fast-growth according to any one of claim 1-3, described
Germanium-containing solution is inorganic germanium solution or organic germanium solution.
7. the method according to claim 6 for improving Caragana Microphylla Seed sprouting and seedling fast-growth, the inorganic germanium solution
For germanium dioxide solution.
8. the method according to claim 6 for improving Caragana Microphylla Seed sprouting and seedling fast-growth, the organic germanium solution
For Ge-132 solution.
9. the method for improving Caragana Microphylla Seed sprouting and seedling fast-growth according to any one of claim 1-3, it is special
Levy and be, in the seed treatment step, the temperature of immersion Caragana Microphylla Seed is 25 DEG C.
10. the method according to claim 9 for improving Caragana Microphylla Seed sprouting and seedling fast-growth, it is characterised in that
The seed is sprouted in step, and the humidity of culture Caragana Microphylla Seed is that 50%, illumination is that 1000lx, temperature are 25 DEG C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710413019.2A CN107018706B (en) | 2017-06-05 | 2017-06-05 | Method for improving germination of caragana microphylla seeds and rapid growth of seedlings |
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| CN110214669A (en) * | 2019-07-08 | 2019-09-10 | 重庆市农业科学院 | A kind of citrus seedling medium and its method for culturing seedlings |
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