CN107014658B - Method and kit for extracting free cells - Google Patents
Method and kit for extracting free cells Download PDFInfo
- Publication number
- CN107014658B CN107014658B CN201710392350.0A CN201710392350A CN107014658B CN 107014658 B CN107014658 B CN 107014658B CN 201710392350 A CN201710392350 A CN 201710392350A CN 107014658 B CN107014658 B CN 107014658B
- Authority
- CN
- China
- Prior art keywords
- solution
- tissue
- distilled water
- stabilizer
- double distilled
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a method and a kit for extracting free cells, wherein the method comprises the following steps: moving tissue on the puncture needle with the tissue tag; washing the tissue in the solution A to obtain free cells; free cells were stored in solution B. The method provided by the invention is used for extracting the episome and analyzing the genome, and can not influence the genome at all, so that the accuracy of the genome analysis result can be improved, and particularly, the problem of RNA degradation is solved; on the other hand, the extracted cell tissues can be fully utilized, so that the waste is avoided, the gene resources are saved to the greatest extent, and the clinical trace precious specimens are utilized (such as prostate puncture and specimens obtained by an endoscope, so that the timely detection of the gene expression condition becomes possible).
Description
Technical Field
The invention relates to the technical field of cell detection, in particular to a method and a kit for extracting cells on free tissues.
Background
In diagnostic cytology or clinical cytology, sloughed off cell examination is the extraction of patient genome and diagnosis of disease from cell morphology. The current method of extracting genome is to aspirate cell tissue from a patient using a syringe-like structure to make paraffin tissue, and then extract the genome from a slice of paraffin tissue. The disadvantages of this are: 1. artificial mutations may be introduced. 2. For certain disease diagnoses, the large number of paraffin sections required, for example, 10 white sections of a micro specimen such as a prostate puncture, may be sufficient for downstream examination. 3. Too much paraffin can interfere with genomic extraction of the tissue. 4. Most of the RNA has degraded, resulting in either failure of downstream detection or inaccurate detection results.
Disclosure of Invention
The invention aims to provide a method and a kit for extracting free cells adhered to tissues.
Therefore, the embodiment of the invention provides the following technical scheme:
a method of extracting free cells comprising the steps of:
moving tissue on the puncture needle with the tissue tag;
washing the tissue in the solution A to obtain free cells;
storing the washed free cells in the solution B;
the solution A is a mixed solution containing NaCl, na2Hpo4, KH2po4, a stabilizer and double distilled water, and the solution B is a mixed solution containing guanidine isothioate, sodium citrate and double distilled water.
According to the examples of the present invention, the contents of the components in 1 liter of the A solution were respectively: nacl=8.5-9 g, na2Hpo 4=11-16 g, KH2 po4=1-4 g, stabilizer=0.2-0.7 g, the rest is double distilled water; alternatively, the contents of the components in 1 liter of the B solution are respectively: guanidine isothioate=9 to 14 g, sodium citrate=0.6 to 2.3 g, stabilizer=0.5 to 1.5 g, and the balance is double distilled water.
A kit, includes the box body, be provided with in the box body:
a first solution EP pipe which contains an A solution, wherein the A solution is a mixed solution containing NaCl, na2Hpo4, KH2po4, a stabilizer and double distilled water;
the second solution EP pipe is filled with a solution B, wherein the solution B is a mixed solution containing guanidine isothioate, sodium citrate, a stabilizer and double distilled water;
the tissue swab is used for moving the tissue on the puncture needle so as to extract free cells on the tissue.
According to the embodiment of the invention, the tissue tag comprises a conical entity with two sealed ends, and concave-convex teeth are arranged on the conical entity and used for introducing free tissue fluid into the EP tube.
Preferably, the kit further comprises a positioning pad, wherein the positioning pad is provided with a first positioning groove, a second positioning groove and a third positioning groove for placing the first solution EP tube, the second solution EP tube and the tissue label respectively.
Compared with the prior art, the invention has the beneficial effects that:
1) After the free cell sap is extracted, the solution A is firstly used for rinsing, and then the solution B is used for preserving so as to prepare the extracted genome, so that the whole process can not damage the genome, can not interfere with the extraction of the genome, and can not degrade RNA.
2) In the prior art, cells adhered to the surface of a micro-tissue to be punctured are lost in a pathological fixing liquid, and the method can fully utilize the cells to extract genome, so that the extracted cell tissue is fully utilized, excessive extraction of the cell tissue from a human body for detection is avoided, and the method also has the advantages of utilizing a clinical micro-precious sample (such as prostate puncture and an endoscope-taken sample, so that timely detection of gene expression condition becomes possible).
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a tissue tag in accordance with an embodiment of the present invention.
FIG. 2 is a schematic diagram of a reagent cartridge according to an embodiment of the present invention.
The marks in the figure:
11-conical solid; 12-concave-convex teeth;
10-a box body; 20-positioning pads; 30-a first positioning groove; 40-a second positioning groove; 50-third positioning groove.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. The components of the embodiments of the present invention generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by a person skilled in the art without making any inventive effort, are intended to be within the scope of the present invention.
In this embodiment, a method for extracting free cell sap is provided, which includes the steps of:
step one: the tissue on the puncture needle is moved by the tissue tag, and free cell sap on the tissue is extracted.
Referring to fig. 1, the tissue tag may be constructed as shown in fig. 1, and includes a hollow cone-shaped body 11, wherein 4-5 concave-convex teeth 12_ are provided on the cone-shaped body 11, and the tissue on the puncture needle is introduced into the EP tube through the concave-convex teeth 12. As an example of application, the tapered body 11 has a thin head diameter of 1mm, a thick head diameter of 2.5 to 3mm and a length of 125mm. Of course, the specific structural dimensions of the conical entity may be designed according to different use scenarios.
Step two: and (3) rinsing the extracted free cell liquid in the solution A. The solution A comprises Nacl, na2Hpo4, KH2po4, stabilizer, and double distilled water (DDH) 2 O) is provided. According to the study, the contents of the components in 1 liter of solution A are respectively: nacl=8.5 to 9 g, na2Hpo 4=11 to 16 g, KH2 po4=1 to 4 g, stabilizer=0.2 to 0.7 gThe balance is double distilled water.
The contents of the components in the solution A were different, and the results of rinsing were affected, as shown in tables 1 and 2.
TABLE 1
TABLE 2
As can be seen from table 2, the free cell extraction was best when in 1 liter of a solution, nacl=8.5 g, na2Hpo4 =13 g, KH2 po4=3 g, stabilizer=0.5 g, and the rest was double distilled water.
Step three: the washed free cells are preserved in the solution B, and the free cells are taken out of the solution B when genome extraction is required. The solution B is a mixed solution containing guanidine isothioate and sodium citrate. According to the study, the contents of the components in 1 liter of the solution B are respectively: guanidine isothioate=9 to 14 g, sodium citrate=0.6 to 2.3 g, stabilizer=0.5 to 1.5 g, and the balance is double distilled water. In particular, 10 g of guanidine isothioate, 1 g of sodium citrate, 0.8 g of stabilizer and the balance of double distilled water in 1 liter of solution B are preferable, as shown in Table 4.
The content of each component in the solution B was different, and the preservation effect of the free cells was affected, as shown in tables 3 and 4.
TABLE 3 Table 3
TABLE 4 Table 4
| Performance of | First group of | Second group of | Third group of | Fourth group | Fifth group of |
| Cell viability | 78％ | 91％ | 64％ | 50％ | 40％ |
| Total RNA (ug) | 0.1611 | 0.4150 | 0.0726 | 0.0513 | 0.0558 |
As can be seen from table 2, the free cell extraction was best when in 1 liter of a solution, nacl=8.5 g, na2Hpo4 =13 g, KH2 po4=3 g, stabilizer=0.5 g, and the rest was double distilled water.
According to the method, the solution A is used for rinsing the tissue to extract the free cells, and the solution B is used for preserving the free cells for extracting the genome, so that the whole process cannot damage the genome, cannot interfere with the extraction of the genome, and cannot degrade RNA.
In the prior art, surface cells penetrating micro tissues are lost in pathological fixing liquid, and the method can fully utilize the part of cells to extract genome, so that the extracted cell tissues are fully utilized, excessive extraction of the cell tissues from a human body for detection is avoided, gene resources are saved to the greatest extent, and timely detection of gene expression is possible for a precious specimen obtained by a clinical endoscope.
Correspondingly, the embodiment also provides a kit, which comprises a box body 10, wherein the box body 10 is internally provided with:
a first solution EP tube containing a solution A for rinsing the tissue and extracting free cells;
a second solution EP tube containing a solution B for preserving the extracted free cells;
a tissue swab, such as the structure shown in fig. 1, for moving tissue on the needle and into the first solution EP tube;
the positioning pad 20, which may also be referred to as a crash pad, is provided with a first positioning groove 30, a second positioning groove 40 and a third positioning groove 50 for placing the first solution EP tube, the second solution EP tube and the tissue label, respectively, so as to prevent the first solution EP tube, the second solution EP tube and the tissue label from being damaged due to vibration during transportation.
The foregoing is merely illustrative of the present invention, and the present invention is not limited thereto, and any person skilled in the art will readily recognize that variations or substitutions are within the scope of the present invention.
Claims (5)
1. A method of extracting free cells comprising the steps of:
moving tissue on the puncture needle with the tissue tag;
washing the tissue in the solution A to obtain free cells;
storing the washed free cells in the solution B;
the solution A is a mixed solution containing NaCl, na2Hpo4, KH2po4, a stabilizer and double distilled water, the solution B is a mixed solution containing guanidine isothioate, sodium citrate and double distilled water, and the contents of the components in 1 liter of solution A are respectively as follows: nacl=8.5-9 g, na2Hpo 4=11-16 g, KH2 po4=1-4 g, stabilizer=0.2-0.7 g, the rest is double distilled water; the contents of each component in 1 liter of the B solution are respectively as follows: guanidine isothioate=9 to 14 g, sodium citrate=0.6 to 2.3 g, stabilizer=0.5 to 1.5 g, and the balance is double distilled water.
2. The kit is characterized by comprising a kit body, wherein the kit body is internally provided with: a first solution EP pipe which contains an A solution, wherein the A solution is a mixed solution containing NaCl, na2Hpo4, KH2po4, a stabilizer and double distilled water; the content of each component in 1 liter of the solution A is respectively as follows: nacl=8.5-9 g, na2Hpo 4=11-16 g, KH2 po4=1-4 g, stabilizer=0.2-0.7 g, the rest is double distilled water;
the second solution EP pipe is filled with a solution B, wherein the solution B is a mixed solution containing guanidine isothioate, sodium citrate, a stabilizer and double distilled water; the contents of each component in 1 liter of the B solution are respectively as follows: guanidine isothioate=9-14 g, sodium citrate=0.6-2.3 g, stabilizer=0.5-1.5 g, and the rest is double distilled water; the tissue swab is used for moving the tissue on the puncture needle so as to extract free cells on the tissue.
3. The kit of claim 2, wherein the tissue tag comprises a conical body provided with concave-convex teeth for introducing free tissue into the EP tube.
4. A kit according to claim 3, wherein the conical entity is a conical entity with a small head diameter of 1mm and a large head diameter of 2.5-3 mm.
5. The kit of claim 2, further comprising a positioning pad provided with a first positioning slot, a second positioning slot and a third positioning slot for placing the first solution EP tube, the second solution EP tube, the tissue tag, respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710392350.0A CN107014658B (en) | 2017-05-27 | 2017-05-27 | Method and kit for extracting free cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710392350.0A CN107014658B (en) | 2017-05-27 | 2017-05-27 | Method and kit for extracting free cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107014658A CN107014658A (en) | 2017-08-04 |
| CN107014658B true CN107014658B (en) | 2023-08-25 |
Family
ID=59451600
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710392350.0A Active CN107014658B (en) | 2017-05-27 | 2017-05-27 | Method and kit for extracting free cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107014658B (en) |
Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1146136A (en) * | 1994-02-14 | 1997-03-26 | 杰纳勒尔医疗公司 | Targeted cytolysis of HIV-infected cells by Chimeric CD4 receptor bearing cells |
| CN1465406A (en) * | 2002-06-21 | 2004-01-07 | 中国科学院化学研究所 | Method for preparing tissue engineering cell scaffold and products obtained by said method |
| CN1967193A (en) * | 2005-11-18 | 2007-05-23 | 上海昂佰生物医学科技有限公司 | Cell processing kit and using method therefor |
| CN101085376A (en) * | 2007-06-07 | 2007-12-12 | 四川省肿瘤医院 | Biological tissue material for artificial esophagus and preparation method thereof |
| JP2008249543A (en) * | 2007-03-30 | 2008-10-16 | Tohoku Univ | Method for preparing cell from tissue sample |
| CN101392019A (en) * | 2008-10-30 | 2009-03-25 | 中国科学院新疆生态与地理研究所 | Isolation and purification method of antifreeze protein from ammopiptanthus monogolicus leaf |
| FR2928004A3 (en) * | 2008-02-27 | 2009-08-28 | Biotesys Gmbh | KIT FOR SAMPLING AND DEVELOPING CELL SAMPLES. |
| CN201575949U (en) * | 2010-01-11 | 2010-09-08 | 成都海兰天澄科技有限公司 | PTC heating digestion device |
| WO2010134073A1 (en) * | 2009-05-19 | 2010-11-25 | Zetiq Technologies Ltd. | Kits for and methods of differential staining of cervical cancer cells and/or tissues |
| JP2011169873A (en) * | 2010-02-22 | 2011-09-01 | Nagasaki Univ | Method of detecting metastasis of cancer and detection kit |
| CN102559579A (en) * | 2010-12-17 | 2012-07-11 | 北京东方润龙投资有限公司 | Novel multi-cell three-dimensional co-culture system for in-vitro detection of newly born blood vessel and kit thereof |
| CN102876663A (en) * | 2012-10-10 | 2013-01-16 | 北京百泰克生物技术有限公司 | Reagent and method for rapidly extracting main RNA (ribonucleic acid) from liquid sample |
| CN103224929A (en) * | 2013-04-09 | 2013-07-31 | 南京紫霄科技有限公司 | Method for rapidly concentrating and extracting nucleic acid target cells from sample |
| CN103875653A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Endocervical cell preserving fluid and method for preparing endocervical cell specimen |
| CN103911366A (en) * | 2014-03-19 | 2014-07-09 | 华南理工大学 | Genome DNA extraction method and its kit |
| CN104111254A (en) * | 2014-07-09 | 2014-10-22 | 李维 | Tumor chemotherapy drug susceptibility detection kit |
| CN104642300A (en) * | 2015-03-13 | 2015-05-27 | 上海御康医院有限公司 | Exfoliative cell sap base preserving fluid, method thereof for flaking and kit |
| CN204485883U (en) * | 2015-02-09 | 2015-07-22 | 孝感宏翔生物医械技术有限公司 | A kind ofly be convenient to rinse the cell-preservation liquid bottle washing cell |
| CN204714570U (en) * | 2015-05-09 | 2015-10-21 | 福建金源泉科技发展有限公司 | A kind of Magnetizing water purifier |
| CN105368820A (en) * | 2015-12-22 | 2016-03-02 | 南京先进激光技术研究院 | Whole blood DNA (deoxyribonucleic acid) extraction kit based on paramagnetic particle method and application of extraction kit |
| CN105713900A (en) * | 2016-03-29 | 2016-06-29 | 广州市玛达生物科技有限公司 | Nucleic acid extraction method based on magnetic graphene nano-composites |
| CN106480017A (en) * | 2016-11-15 | 2017-03-08 | 武汉海吉力生物科技有限公司 | Extract the test kit of circulating tumor cell DNA and tumor dissociative DNA simultaneously |
| CN206848021U (en) * | 2017-05-27 | 2018-01-05 | 四川省肿瘤医院 | Extract the kit of free cell |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9322065B2 (en) * | 2007-09-17 | 2016-04-26 | Mdxhealth Sa | Methods for determining methylation of the TWIST1 gene for bladder cancer detection |
| WO2010089538A2 (en) * | 2009-02-03 | 2010-08-12 | Oncomethylome Sciences Sa | Methods of detecting colorectal cancer |
| US20140120068A1 (en) * | 2012-11-01 | 2014-05-01 | Animal Cell Therapies, Inc. | Solutions, systems and methods for cell, tissue and organ preservation |
-
2017
- 2017-05-27 CN CN201710392350.0A patent/CN107014658B/en active Active
Patent Citations (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1146136A (en) * | 1994-02-14 | 1997-03-26 | 杰纳勒尔医疗公司 | Targeted cytolysis of HIV-infected cells by Chimeric CD4 receptor bearing cells |
| CN1465406A (en) * | 2002-06-21 | 2004-01-07 | 中国科学院化学研究所 | Method for preparing tissue engineering cell scaffold and products obtained by said method |
| CN1967193A (en) * | 2005-11-18 | 2007-05-23 | 上海昂佰生物医学科技有限公司 | Cell processing kit and using method therefor |
| JP2008249543A (en) * | 2007-03-30 | 2008-10-16 | Tohoku Univ | Method for preparing cell from tissue sample |
| CN101085376A (en) * | 2007-06-07 | 2007-12-12 | 四川省肿瘤医院 | Biological tissue material for artificial esophagus and preparation method thereof |
| FR2928004A3 (en) * | 2008-02-27 | 2009-08-28 | Biotesys Gmbh | KIT FOR SAMPLING AND DEVELOPING CELL SAMPLES. |
| CN101392019A (en) * | 2008-10-30 | 2009-03-25 | 中国科学院新疆生态与地理研究所 | Isolation and purification method of antifreeze protein from ammopiptanthus monogolicus leaf |
| WO2010134073A1 (en) * | 2009-05-19 | 2010-11-25 | Zetiq Technologies Ltd. | Kits for and methods of differential staining of cervical cancer cells and/or tissues |
| CN201575949U (en) * | 2010-01-11 | 2010-09-08 | 成都海兰天澄科技有限公司 | PTC heating digestion device |
| JP2011169873A (en) * | 2010-02-22 | 2011-09-01 | Nagasaki Univ | Method of detecting metastasis of cancer and detection kit |
| CN102559579A (en) * | 2010-12-17 | 2012-07-11 | 北京东方润龙投资有限公司 | Novel multi-cell three-dimensional co-culture system for in-vitro detection of newly born blood vessel and kit thereof |
| CN102876663A (en) * | 2012-10-10 | 2013-01-16 | 北京百泰克生物技术有限公司 | Reagent and method for rapidly extracting main RNA (ribonucleic acid) from liquid sample |
| CN103224929A (en) * | 2013-04-09 | 2013-07-31 | 南京紫霄科技有限公司 | Method for rapidly concentrating and extracting nucleic acid target cells from sample |
| CN103875653A (en) * | 2014-02-11 | 2014-06-25 | 中国人民解放军北京军区总医院 | Endocervical cell preserving fluid and method for preparing endocervical cell specimen |
| CN103911366A (en) * | 2014-03-19 | 2014-07-09 | 华南理工大学 | Genome DNA extraction method and its kit |
| CN104111254A (en) * | 2014-07-09 | 2014-10-22 | 李维 | Tumor chemotherapy drug susceptibility detection kit |
| CN204485883U (en) * | 2015-02-09 | 2015-07-22 | 孝感宏翔生物医械技术有限公司 | A kind ofly be convenient to rinse the cell-preservation liquid bottle washing cell |
| CN104642300A (en) * | 2015-03-13 | 2015-05-27 | 上海御康医院有限公司 | Exfoliative cell sap base preserving fluid, method thereof for flaking and kit |
| CN204714570U (en) * | 2015-05-09 | 2015-10-21 | 福建金源泉科技发展有限公司 | A kind of Magnetizing water purifier |
| CN105368820A (en) * | 2015-12-22 | 2016-03-02 | 南京先进激光技术研究院 | Whole blood DNA (deoxyribonucleic acid) extraction kit based on paramagnetic particle method and application of extraction kit |
| CN105713900A (en) * | 2016-03-29 | 2016-06-29 | 广州市玛达生物科技有限公司 | Nucleic acid extraction method based on magnetic graphene nano-composites |
| CN106480017A (en) * | 2016-11-15 | 2017-03-08 | 武汉海吉力生物科技有限公司 | Extract the test kit of circulating tumor cell DNA and tumor dissociative DNA simultaneously |
| CN206848021U (en) * | 2017-05-27 | 2018-01-05 | 四川省肿瘤医院 | Extract the kit of free cell |
Non-Patent Citations (1)
| Title |
|---|
| Establishment of a xeno-free culture system that preserves the characteristics of placenta mesenchymal stem cells;Saeyoung Park 等;《Cytotechnology》(第67期);第851-860页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107014658A (en) | 2017-08-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Systematic comparison of bacterial colonization of endometrial tissue and fluid samples in recurrent miscarriage patients: implications for future endometrial microbiome studies | |
| CN107881213A (en) | A kind of urine preserves reagent and its application | |
| CN105368817B (en) | Cervical cell preservation and DNA rapid extraction one kits and extracting method | |
| CN101363011A (en) | Cervical exfoliated cell preservative fluid | |
| WO2010122562A1 (en) | A sperm separation system | |
| CN107267500A (en) | A kind of dissociative DNA preserves liquid and its preparation method and application | |
| EP2694942B1 (en) | Method for preparing cytological specimens for analyses | |
| Garcia-Vazquez et al. | Neospora caninum seropositivity and association with abortions in dairy cows in Mexico | |
| JP2015503756A5 (en) | ||
| CN104041484A (en) | Cell preservation liquid | |
| EP2596121A4 (en) | Method for linking point of care rapid diagnostic testing results to laboratory-based methods | |
| CN107014658B (en) | Method and kit for extracting free cells | |
| CN103243068A (en) | Method for rapidly evaluating composition effect of estrogen and antiestrogen | |
| Simons et al. | Effects of histological staining on the analysis of human DNA from archived slides | |
| Leme et al. | How to perform and interpret testicular fine needle aspiration in stallions | |
| CN206848021U (en) | Extract the kit of free cell | |
| CN113373031B (en) | Spray type free DNA sample storage tube and application | |
| CN108776165A (en) | The acid polyacrylamide gel electrophoresis separation method of Magnoliacea plant peroxidase | |
| Song et al. | Technical aspect of ThinPrep | |
| Anchana et al. | Cytomorphologic analysis of wet fixed and air dried oral buccal smears rehydrated with normal saline: A comparative study | |
| WO2021243444A1 (en) | At-home kit for prostate cancer screening and other diseases | |
| CN203405623U (en) | Novel internal medicine microscope test film | |
| US20150050683A1 (en) | Device And Method | |
| KR102563528B1 (en) | Solution composition for liquid based cytology of cervical cancer | |
| Bakhiet et al. | Fine needle aspiration cytology utility in the identification of Mycetoma causative agents: The Mycetoma Research Center experience. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |