CN107006265B - Inoculating device for agaricus blazei murill - Google Patents
Inoculating device for agaricus blazei murill Download PDFInfo
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- CN107006265B CN107006265B CN201710335565.9A CN201710335565A CN107006265B CN 107006265 B CN107006265 B CN 107006265B CN 201710335565 A CN201710335565 A CN 201710335565A CN 107006265 B CN107006265 B CN 107006265B
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- piston
- cavity
- inoculation
- piston rod
- inoculation box
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- 241001327634 Agaricus blazei Species 0.000 title claims abstract description 15
- 238000011081 inoculation Methods 0.000 claims abstract description 70
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000007921 spray Substances 0.000 claims description 14
- 230000004083 survival effect Effects 0.000 abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 17
- 239000001301 oxygen Substances 0.000 description 17
- 229910052760 oxygen Inorganic materials 0.000 description 17
- 239000007788 liquid Substances 0.000 description 16
- 238000007664 blowing Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 206010016717 Fistula Diseases 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 241000220304 Prunus dulcis Species 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 230000003890 fistula Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 208000014617 hemorrhoid Diseases 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- LUEWUZLMQUOBSB-GFVSVBBRSA-N mannan Chemical class O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-GFVSVBBRSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The application discloses an inoculation device for agaricus blazei murill, including the inoculation box, the inoculation box is embedded to have L shape division board and the first piston of horizontal setting, first piston is located the top of division board, constitute first cavity between division board and the inoculation box right side inner wall, constitute the second cavity between division board and the inner wall of inoculation box left side and bottom, first piston is connected with first piston rod, first piston rod stretches out outside the inoculation box, first cavity is equipped with the second piston, second piston is connected with the second piston rod, be equipped with the third piston in the second cavity, third piston is connected with the third piston rod, second piston rod and third piston rod all are connected with first piston, the intercommunication has the shower nozzle on the second cavity, the second cavity is located the second piston below and is equipped with the inlet, be equipped with the nozzle on the third cavity, third cavity is located the third piston below and is equipped with the air inlet, nozzle and shower nozzle are located the same side of inoculation box, air inlet and inlet all are provided with the sealing door. The device can improve the survival rate of the strain.
Description
Technical Field
The invention relates to the field of mushroom planting, in particular to an inoculation device for agaricus blazei murill.
Background
Agaricus blazei murill is originally produced in Brazil and Peru. The preserved fungus growing in summer and autumn is a preserved fungus growing in summer and autumn, living in a high-temperature, high-humidity and ventilated environment, has almond fragrance and is crisp and tender in taste. The agaricus blazei murill has tender fungus cover, crisp fungus handle, good taste and pure taste of 5-7 g; the protein composition comprises 18 amino acids, 8 essential amino acids of human body are complete, and multiple vitamins and ergosterol are also contained. The mannans contained in the composition have the curative effects of inhibiting tumors (especially ascites cancers), treating hemorrhoids and fistulas, enhancing energy, preventing and treating cardiovascular diseases and the like.
The current agaricus blazei murill inoculation method mainly adopts an inoculation spoon to inoculate, the strains which are obtained by digging liquid by the inoculation spoon are placed in a culture bag, oxygen is blown into the culture bag after inoculation is completed to meet the later growth of the strains, but the inoculation and the oxygen blowing are relatively independent working processes, the inoculated culture bag also needs to wait for a period of time to be blown with oxygen, the culture bag is exposed in the air for a long time, bacteria in the air enter the culture bag, the growth of the strains is influenced by the bacteria, and the survival rate is reduced.
Disclosure of Invention
The present invention is intended to provide an inoculating device for agaricus blazei murill to solve the problem of bacteria in the air entering into the culture bag in the prior art.
In order to achieve the above purpose, the basic scheme provided by the application is as follows: the utility model provides an inoculation device for agaricus blazei murill, including the inoculation box, inoculation box embeds has L shape division board and the horizontal first piston that sets up, first piston is located the top of division board, constitute first cavity between division board and the inoculation box right side inner wall, constitute the second cavity between division board and the inner wall of inoculation box left side and bottom, first piston is connected with first piston rod, first piston rod stretches out outside the inoculation box, first cavity is equipped with the second piston, the second piston is connected with the second piston rod, be equipped with the third piston in the second cavity, the third piston is connected with the third piston rod, second piston rod and third piston rod all are connected with first piston, the intercommunication has the shower nozzle on the second cavity, the second cavity is located the second piston below and is equipped with the inlet, be equipped with the nozzle on the third cavity, third cavity is located the same side of inoculation box below the nozzle and shower nozzle, inlet and inlet all are provided with the sealing door.
The working principle of the invention is as follows: before inoculating the culture bag by using the device in the scheme, opening sealing doors of the air inlet and the liquid inlet, adding liquid strains into the first cavity, simultaneously blowing oxygen into the second cavity, and closing the sealing doors; the bag mouth of the culture bag covers the nozzle and the spray head positioned on the same side of the inoculation box, the nozzle and the spray head are wrapped by the bag mouth, the first piston rod is pressed downwards, the first piston moves up and down in the inoculation box, the second piston rod and the third piston rod are connected with the first piston rod, the second piston rod enables the second piston to move in the second cavity under the driving of the first piston rod, the second piston extrudes liquid strains in the first cavity, the spray head is opened under the action of a certain pressure of liquid, and the liquid is sprayed into the culture bag through the spray head; simultaneously, the third piston rod enables the third piston to move in the second cavity, oxygen in the second cavity is extruded, the nozzle is opened under the action of a certain pressure of the oxygen, the oxygen enters the culture bag through the nozzle, the first piston rod is stopped being pressed, the bag opening of the culture bag is tightly tied, and then inoculation and blowing of the culture bag are completed.
The invention has the beneficial effects that: 1. in the scheme, the strain liquid is sprayed into the culture bag by the spray head, and compared with the manual strain smearing in the culture bag, the strain liquid is atomized after passing through the spray head, so that the inoculation is more uniform. 2. The first piston rod is pressed downwards to drive the second piston and the third piston to move, so that the strain liquid and oxygen can enter the culture bag at the same time, the culture bag is not contacted with air, and bacteria in the air cannot enter the culture bag, so that the survival rate of the strain is affected.
In the first scheme, for optimizing the basic scheme, the inoculation box is communicated with an inoculation pipe, and the spray head and the spray nozzle are both positioned in the inoculation pipe. In order to prevent air from entering the culture bag in the process of inoculation and oxygen blowing, the optimal scheme is designed, the inoculation pipe is wrapped by the bag opening of the culture bag, and the inoculation pipe is tied by a wire, so that air can be prevented from entering the culture bag.
And in the second scheme, for optimizing the first scheme, a one-way valve is arranged in the inoculation pipe. The air current in the check valve can only flow towards a direction and can not reverse flow, designs this optimal scheme, and water smoke and oxygen in the inoculation pipe can enter into the culture bag through the check valve, after the inoculation of a culture bag is accomplished and the oxygen is blown in, take off the culture bag from the inoculation pipe, the outside air of inoculation pipe can not enter into the inoculation intraductal through the check valve, when carrying out the preparation of next culture bag, does not have the air to enter into the culture bag through the inoculation pipe in, influences the survival rate of bacterial.
The third scheme is the optimization of the second scheme, the inoculation box is connected with a supporting plate, and the supporting plate is positioned below the inoculation pipe. The support plate can hold up the culture bag, so that the bag mouth of the culture bag is conveniently sleeved on the inoculation pipe.
In a fourth aspect, for optimizing the above aspects, the L-shaped partition plate includes a riser and a transverse plate, and an opening is provided below the riser located on the second piston. When carrying out the fluid infusion to the second cavity, upwards pulling first piston rod, first piston rod drives the second piston rod and removes messenger's second piston in the second cavity, forms the negative pressure in the second cavity, and the air pressure outside the inoculation case is big, and the nozzle can be jacked to the air like this, and the air enters into in the second cavity through the nozzle, so this optimal scheme of design, set up the opening in the below that the division board is located the second piston, when the second piston removes the open-ended top, in the oxygen in the second cavity automatic entering into first cavity, prevents to produce the negative pressure in the first cavity.
Drawings
FIG. 1 is a schematic view showing the structure of an inoculating device for agaricus blazei of the present invention;
FIG. 2 is a schematic cross-sectional view of an inoculating device for agaricus blazei of the present invention.
Detailed Description
The invention is described in further detail below by way of specific embodiments:
reference numerals in the drawings of the specification include: the device comprises an air inlet 1, a first piston rod 2, a liquid inlet 3, an inoculation pipe 4, a supporting plate 5, an inoculation box 6, a third piston 7, a third piston rod 8, a first piston 9, a second piston rod 10, a second piston 11, a spray head 12, a one-way valve 13, a spray nozzle 14, a separation plate 15 and an opening 16.
The embodiment is basically as shown in fig. 1 and 2:
the utility model provides an inoculation device for agaricus blazei murill, including inoculation box 6, inoculation box 6 is internal vertically to be inlayed with L shape division board 15 and the first piston 9 of horizontal setting, first piston 9 is located the top of division board 15, constitute first cavity between division board 15 and the inner wall of inoculation box 6 right side, constitute the second cavity between division board 15 and the inner wall of inoculation box 6 left side and bottom, first piston 9 is connected with first piston rod 2, first piston rod 2 stretches out outside inoculation box 6, first cavity is equipped with second piston 11, second piston 11 is connected with second piston rod 10, be equipped with third piston 7 in the second cavity, third piston 7 is connected with third piston rod 8, second piston rod 10 and third piston rod 8 all are connected with first piston 9, the intercommunication has shower nozzle 12 on the second cavity, second cavity is located second piston 11 below and is equipped with inlet 3, be equipped with nozzle 14 on the third cavity, third cavity is located third piston 7 below and is equipped with air inlet 1, 14 and shower nozzle 12 are located the same side of inoculation box 6, inoculation box 6 is connected with the pipe 12 and is equipped with the third piston 4, the pipe 4 is equipped with the shower nozzle 4 and is located the inlet 13 in the pipe 4, the pipe is located the pipe is connected with the 3, the pipe is equipped with the one-way opening is located 4 in the 3 side of the inoculation pipe is located to the 3, the pipe is still connected with the shower nozzle 4, the top is located 5 is located the 5 to the 5.
Before inoculating the culture bag by using the device in the scheme, opening sealing doors of the air inlet 1 and the liquid inlet 3, adding liquid strains into the first cavity, simultaneously blowing oxygen into the second cavity, and closing the sealing doors; the culture bag is placed on the supporting plate 5, the inoculation pipe 4 is wrapped by the bag opening of the culture bag, the first piston rod 2 is pressed downwards, the first piston 9 moves in the inoculation box 6, the second piston rod 10 and the third piston rod 8 are connected with the first piston rod 2, the second piston rod 10 enables the second piston 11 to move in the coating cavity under the driving of the first piston rod 2, the second piston 11 extrudes liquid strains in the second cavity, the spray head 12 is opened under the action of a certain liquid pressure, and liquid is sprayed into the culture bag through the spray head 12; simultaneously, the third piston rod 8 enables the third piston 7 to move in the second cavity, oxygen in the second cavity is extruded, the nozzle 14 is opened under the action of a certain pressure of the oxygen, the oxygen enters the culture bag through the nozzle 14, the first piston rod 2 is stopped being pressed, the mouth of the culture bag is tied tightly, and inoculation and blowing of the culture bag are completed.
When the culture bag is taken down from the inoculation pipe 4, the air outside the inoculation pipe 4 cannot enter the inoculation pipe 4 through the one-way valve 13; when the first chamber is replenished with liquid, the oxygen in the second chamber automatically enters the first chamber when the second piston 11 moves above the opening 16, so that negative pressure is prevented from being generated in the first chamber.
The foregoing is merely exemplary of the present invention, and specific structures and features that are well known in the art are not described in detail herein. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present invention, and these should also be considered as the scope of the present invention, which does not affect the effect of the implementation of the present invention and the utility of the patent. The protection scope of the present application shall be subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (3)
1. An inoculating device for agaricus blazei murill, characterized in that: including the inoculation box, the inoculation box is embedded to have L shape division board and the first piston of horizontal setting, first piston is located the top of division board, constitute first cavity between division board and the inoculation box right side inner wall, constitute the second cavity between division board and the inner wall of inoculation box left side and bottom, first piston is connected with first piston rod, first piston rod stretches out outside the inoculation box, first cavity is equipped with the second piston, second piston is connected with the second piston rod, be equipped with the third piston in the second cavity, the third piston is connected with the third piston rod, second piston rod and third piston rod all are connected with first piston, the intercommunication has the shower nozzle on the second cavity, the second cavity is located the second piston below and is equipped with the inlet, be equipped with the nozzle on the third cavity, third cavity is located the third piston below and is equipped with the air inlet, nozzle and shower nozzle are located the same side of inoculation box, air inlet and inlet all are provided with the sealing door, the division board is located the below of second piston still equipped with the opening, be equipped with the check valve in the inoculation pipe.
2. An inoculating device for agaricus blazei according to claim 1, wherein: the inoculation box is communicated with an inoculation pipe, and the spray head and the spray nozzle are both positioned in the inoculation pipe.
3. An inoculating device for agaricus blazei according to claim 2, wherein: the inoculation box is connected with the backup pad, the backup pad is located the inoculation pipe below.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710335565.9A CN107006265B (en) | 2017-05-12 | 2017-05-12 | Inoculating device for agaricus blazei murill |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710335565.9A CN107006265B (en) | 2017-05-12 | 2017-05-12 | Inoculating device for agaricus blazei murill |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107006265A CN107006265A (en) | 2017-08-04 |
| CN107006265B true CN107006265B (en) | 2024-01-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710335565.9A Active CN107006265B (en) | 2017-05-12 | 2017-05-12 | Inoculating device for agaricus blazei murill |
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| Country | Link |
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| CN (1) | CN107006265B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109168994B (en) * | 2018-11-15 | 2021-04-30 | 习水县三水田园现代农业有限公司 | Inoculation device of glossy ganoderma fungus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202680036U (en) * | 2012-01-18 | 2013-01-23 | 山西强盛种业有限公司 | Edible mushroom liquid spawn inoculation injection gun and inoculation system |
| KR101246238B1 (en) * | 2011-11-29 | 2013-03-21 | 안용호 | Automatic inoculation equipment for mushroom spawn |
| CN204994301U (en) * | 2015-09-23 | 2016-01-27 | 绿源永乐(北京)农业科技发展有限公司 | Novel mushroom strain inoculator |
| CN207340815U (en) * | 2017-05-12 | 2018-05-11 | 贵州宏华农业发展有限公司 | A kind of classification inoculation apparatus for Agricus blazei |
-
2017
- 2017-05-12 CN CN201710335565.9A patent/CN107006265B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101246238B1 (en) * | 2011-11-29 | 2013-03-21 | 안용호 | Automatic inoculation equipment for mushroom spawn |
| CN202680036U (en) * | 2012-01-18 | 2013-01-23 | 山西强盛种业有限公司 | Edible mushroom liquid spawn inoculation injection gun and inoculation system |
| CN204994301U (en) * | 2015-09-23 | 2016-01-27 | 绿源永乐(北京)农业科技发展有限公司 | Novel mushroom strain inoculator |
| CN207340815U (en) * | 2017-05-12 | 2018-05-11 | 贵州宏华农业发展有限公司 | A kind of classification inoculation apparatus for Agricus blazei |
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| Publication number | Publication date |
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| CN107006265A (en) | 2017-08-04 |
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