CN107002120B - 测序方法 - Google Patents

测序方法 Download PDF

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Publication number
CN107002120B
CN107002120B CN201580039895.2A CN201580039895A CN107002120B CN 107002120 B CN107002120 B CN 107002120B CN 201580039895 A CN201580039895 A CN 201580039895A CN 107002120 B CN107002120 B CN 107002120B
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China
Prior art keywords
nucleic acid
template nucleic
sequence
acid molecules
molecular marker
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English (en)
Chinese (zh)
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CN107002120A (zh
Inventor
凯瑟琳·默里·伯克
艾伦·厄尔·达尔林
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Illumina Singapore Pte Ltd
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Langs Technology Co ltd
University of Technology Sydney
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51177418&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN107002120(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Langs Technology Co ltd, University of Technology Sydney filed Critical Langs Technology Co ltd
Priority to CN202210864432.1A priority Critical patent/CN116072218A/zh
Publication of CN107002120A publication Critical patent/CN107002120A/zh
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    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/20—Polymerase chain reaction [PCR]; Primer or probe design; Probe optimisation
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Theoretical Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioethics (AREA)
  • Databases & Information Systems (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN201580039895.2A 2014-05-23 2015-05-22 测序方法 Active CN107002120B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210864432.1A CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1409282.9 2014-05-23
GBGB1409282.9A GB201409282D0 (en) 2014-05-23 2014-05-23 Sequencing process
PCT/GB2015/051518 WO2015177570A1 (en) 2014-05-23 2015-05-22 Sequencing process

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CN202210864432.1A Division CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Publications (2)

Publication Number Publication Date
CN107002120A CN107002120A (zh) 2017-08-01
CN107002120B true CN107002120B (zh) 2022-08-09

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CN201580039895.2A Active CN107002120B (zh) 2014-05-23 2015-05-22 测序方法
CN202210864432.1A Pending CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

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CN202210864432.1A Pending CN116072218A (zh) 2014-05-23 2015-05-22 测序方法

Country Status (11)

Country Link
US (2) US11047002B2 (cg-RX-API-DMAC10.html)
EP (1) EP3146070B2 (cg-RX-API-DMAC10.html)
JP (1) JP6687605B2 (cg-RX-API-DMAC10.html)
KR (3) KR102864537B1 (cg-RX-API-DMAC10.html)
CN (2) CN107002120B (cg-RX-API-DMAC10.html)
AU (1) AU2015263055B2 (cg-RX-API-DMAC10.html)
DK (1) DK3146070T5 (cg-RX-API-DMAC10.html)
ES (1) ES2697804T5 (cg-RX-API-DMAC10.html)
GB (1) GB201409282D0 (cg-RX-API-DMAC10.html)
SG (1) SG11201609754QA (cg-RX-API-DMAC10.html)
WO (1) WO2015177570A1 (cg-RX-API-DMAC10.html)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
CN109906274B (zh) * 2016-11-08 2023-08-25 贝克顿迪金森公司 用于细胞标记分类的方法
AU2017359048B2 (en) 2016-11-08 2022-07-21 Becton, Dickinson And Company Methods for expression profile classification
KR101798229B1 (ko) * 2016-12-27 2017-12-12 주식회사 천랩 전장 리보솜 rna 서열정보를 얻는 방법 및 상기 리보솜 rna 서열정보를 이용하여 미생물을 동정하는 방법
CN111108218B (zh) 2017-09-20 2024-06-25 生命科技股份有限公司 使用压缩的分子标记的核酸序列数据检测融合的方法
WO2019060804A1 (en) * 2017-09-25 2019-03-28 Cellular Research, Inc. IMMUNE BAR CODE RECEIVER ERROR CORRECTION
KR102878365B1 (ko) * 2017-10-09 2025-10-31 주식회사 마크로젠 단일 분자 서열분석 및 핵산서열 특성화를 위한 고유한 분자 식별자
WO2019132010A1 (ja) * 2017-12-28 2019-07-04 タカラバイオ株式会社 塩基配列における塩基種を推定する方法、装置及びプログラム
US12221657B2 (en) 2018-08-10 2025-02-11 Tata Consultancy Services Limited Method and system for improving amplicon sequencing based taxonomic resolution of microbial communities
EP3710597B1 (en) * 2018-08-13 2021-07-14 Longas Technologies Pty Ltd Sequencing algorithm
US12651646B2 (en) 2021-07-23 2026-06-09 Illumina, Inc. Machine-learning model for recalibrating nucleotide-base calls
WO2025255494A1 (en) 2024-06-07 2025-12-11 Illumina, Inc. Identifying the origin of amplified rna molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137734A1 (en) * 2005-06-23 2006-12-28 Keygene N.V. Improved strategies for sequencing complex genomes using high throughput sequencing technologies

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ336770A (en) * 1997-01-15 2001-01-26 Brax Group Ltd Enzymatic method and kit for sequencing DNA
JP4403069B2 (ja) * 2002-08-12 2010-01-20 独立行政法人理化学研究所 クローニングおよび分析のためのｍＲＮＡの５’末端の使用方法
JP4413017B2 (ja) * 2004-01-15 2010-02-10 独立行政法人理化学研究所 クラスタリングプログラムおよびクラスタリング装置
JP5415264B2 (ja) * 2006-06-30 2014-02-12 ドイスコベルク コーポレーション 検出可能な核酸タグ
EP2513333B1 (en) 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
DK2599877T3 (da) 2010-06-30 2017-11-20 Bgi Genomics Co Ltd Hidtil ukendt pcr sekvenseringsfremgangsmåde og anvendelse deraf i hla-genotypebestemmelse
US9033273B2 (en) 2010-11-02 2015-05-19 Jonathan Edelson Integrated aircraft ground navigation control system
EP3246416B1 (en) 2011-04-15 2024-06-05 The Johns Hopkins University Safe sequencing system
SG10201605049QA (en) * 2011-05-20 2016-07-28 Fluidigm Corp Nucleic acid encoding reactions
WO2013130512A2 (en) 2012-02-27 2013-09-06 The University Of North Carolina At Chapel Hill Methods and uses for molecular tags
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
CA2871505C (en) 2012-04-24 2021-10-12 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
WO2014047556A1 (en) 2012-09-21 2014-03-27 The Broad Institute, Inc. Compositions and methods for long insert, paired end libraries of nucleic acids in emulsion droplets

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006137734A1 (en) * 2005-06-23 2006-12-28 Keygene N.V. Improved strategies for sequencing complex genomes using high throughput sequencing technologies

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Development of a Dual-Index Sequencing Strategy and Curation Pipeline for Analyzing Amplicon Sequence Data on the MiSeq Illumina Sequencing Platform;Kozich, James J.; Westcott, Sarah L.; Baxter, Nielson T.; 等.;《APPLIED AND ENVIRONMENTAL MICROBIOLOGY》;20130930;第79卷(第17期);全文 *
Microbial diversity in the sputum of a cystic fibrosis patient studied with 16S rDNA pyrosequencing;Armougom, F.; Bittar, F.; Stremler, N.; 等.;《EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES》;20090930;第28卷(第9期);全文 *
The genome of Clostridium difficile 5.3;Darling, Aaron E.; Worden, Paul; Chapman, Toni A.; 等;《GUT PATHOGENS》;20140224;第6卷;Methods、Quality assurance和Initial findings部分 *

Also Published As

Publication number Publication date
CN116072218A (zh) 2023-05-05
AU2015263055A1 (en) 2016-12-08
ES2697804T5 (es) 2024-11-25
WO2015177570A1 (en) 2015-11-26
GB201409282D0 (en) 2014-07-09
KR20230141873A (ko) 2023-10-10
EP3146070B1 (en) 2018-09-26
US11047002B2 (en) 2021-06-29
JP2017517282A (ja) 2017-06-29
EP3146070B2 (en) 2024-07-31
CA2949925A1 (en) 2015-11-26
KR102363284B1 (ko) 2022-02-15
SG11201609754QA (en) 2016-12-29
JP6687605B2 (ja) 2020-04-22
KR102864537B1 (ko) 2025-09-24
ES2697804T3 (es) 2019-01-28
DK3146070T5 (en) 2019-01-21
AU2015263055B2 (en) 2020-12-03
KR20220025213A (ko) 2022-03-03
DK3146070T3 (en) 2019-01-07
CN107002120A (zh) 2017-08-01
US20170183724A1 (en) 2017-06-29
US20210403991A1 (en) 2021-12-30
EP3146070A1 (en) 2017-03-29
KR20170012390A (ko) 2017-02-02
KR102579902B1 (ko) 2023-09-18

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