CN106990801A - Citrate buffer piece and preparation method thereof - Google Patents
Citrate buffer piece and preparation method thereof Download PDFInfo
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- CN106990801A CN106990801A CN201510467866.8A CN201510467866A CN106990801A CN 106990801 A CN106990801 A CN 106990801A CN 201510467866 A CN201510467866 A CN 201510467866A CN 106990801 A CN106990801 A CN 106990801A
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- citrate buffer
- buffer piece
- disintegrant
- auxiliary material
- adhesive
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Abstract
It is circular or square sheet the present invention relates to a kind of Citrate buffer piece and preparation method thereof, can be made into other such as oval, the thin slice such as rectangle, rhombus.Citrate buffer piece prepared by the application belongs to model of instant use once to turn it on, as long as in the solvent of fixed volume, putting into tablet, can just dissolve rapidly, you can the cushioning liquid of the pH value accurate stable needed for obtaining, using and its it is easy, the duplication of labour is reduced, labor intensity is reduced, operating efficiency is improved.
Description
Technical field
The invention belongs to chemicals technical field, it is related to the auxiliary examination in biology, medical science and chemical test
Agent, specifically provides a kind of Citrate buffer piece and preparation method thereof.
Background technology
PH biological buffers system is played an important role to the normal ph and normal physiological context that maintain biology.
Most cells are only capable of the carry out activity in the range of very narrow pH, and need buffer system and be metabolized to resist
The pH changes occurred in journey.
Citrate buffer system, abbreviation CPBS, not still buffer system important in biosystem, and
It is that enzyme is extracted, DNA is extracted, antigen hot repair is multiple, immunological testing, biological experiment, immunochemical technique
There is extensive purposes Deng field.Citrate buffer solution, is based on citrate, according to different
Demand, adds different auxiliary material, forms different pH buffer system.Generally require to be prepared into solution when using,
It is corrected with pH meter, formality is numerous and diverse.Citrate belongs to acylate, the easy moisture absorption, easily grows bacterium,
Make solution very perishable, the holding time is short.So the citrate that can be preserved for a long time for setting up a kind of stabilization delays
Punching agent system is extremely important, can not only save the time, and can preserve for a long time, plug and play.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art part there is provided a kind of plug and play, be easy to storage
The simple and quick Citrate buffer piece of keeping, preparation method.
The present invention realizes that the technical scheme of purpose is as follows：
A kind of Citrate buffer piece, is circular, square, rhombus thin slice.
Moreover, the constituent of the thin slice is：Major ingredient, auxiliary material, adhesive, disintegrant.
Moreover, the major ingredient is citric acid；The auxiliary material is disodium hydrogen phosphate；The disintegrant is crosslinking straight chain
Starch, microcrystalline cellulose or PVPP；Described adhesive is cross-linked amylose, microcrystalline cellulose, gathered
Vinylpyrrolidone, saponified polyvinyl alcohol or amylopectin.
Moreover, the thickness of the thin slice is 1-10mm.
Moreover, having bubble in the thin slice.
Beneficial effects of the present invention are as follows：
1st, Citrate buffer piece prepared by the application belongs to model of instant use once to turn it on, as long as in the solvent of fixed volume,
Tablet is put into, can just be dissolved rapidly, you can the cushioning liquid of the pH value accurate stable needed for obtaining, is used
And its it is easy, the duplication of labour is reduced, labor intensity is reduced, operating efficiency is improved.
2nd, the citrate tablet that the application is provided is easy to storage and custody, and be easy to carry transport, is easy to sealed bundle
Dress, extends pot-life, reduction production and use cost.
3rd, citrate tablet that the application is provided prepares simple and quick, and preparation process is not related to HTHP
Reaction, can carry out industrialized production.
Brief description of the drawings
Fig. 1 is structural representation of the invention：
Fig. 2 is the internal structural representation with alveolate buffer substrate tablet；
Fig. 3 is another structural representation of the invention；
Fig. 4 is preparation of sections artwork of the present invention.
Embodiment
Below in conjunction with the accompanying drawings and the invention will be further described by specific embodiment, following examples are to retouch
The property stated, be not limited, it is impossible to limit protection scope of the present invention with this.
A kind of Citrate buffer piece, is circular 1 or square 3 thin slice, can be made into other such as ellipses,
The thin slices such as rectangle, rhombus, in order to increase dissolution velocity, fill minute bubbles 2 in circular or square sheet,
As Figure 1-3.Wherein the thickness of thin slice is that 1-10mm is the most suitable, so dissolves very fast.
Preparation of sections method and step is as follows：
1st, purifying major ingredient, auxiliary material, adhesive, disintegrant, make to meet biological classes and grades in school, wherein major ingredient-citric acid,
Auxiliary material-disodium hydrogen phosphate, disintegrant-cross-linked amylose, microcrystalline cellulose, PVPP etc., adhesive
- cross-linked amylose, microcrystalline cellulose, polyvinylpyrrolidone, partly-hydrolysed polyvinyl alcohol, amylopectin
Deng；
2nd, precise major ingredient, auxiliary material, adhesive, disintegrant；
3rd, matching mixing, stirs；
4th, tablet press machine pressure is harmonized, tabletting, pressure is 2-4 tons, is weighed, test passes, packaging.
Several specific method for preparing slices presented below
Embodiment 1
A kind of pH5.5 citrate (CPBS) piece, preparation method is as follows：
(1) it is 5.3 by weight:17.8:5:0.9 ratio, weigh respectively successively citric acid, disodium hydrogen phosphate,
If each dry weight of potassium chloride, cross-linked amylose, for example：Citric acid 53g, disodium hydrogen phosphate 178g are weighed,
Potassium chloride 50g, cross-linked amylose 9g, matching is mixed evenly；
(2) tabletted on tablet press machine, pressing pressure is 4 tons, compacting 100, every weight 2.9g,
Containing citric acid, sodium dihydrogen phosphate, potassium chloride, cross-linked amylose, its part by weight is followed successively by
5.3:17.8:5:0.9。
In use, every is dissolved in 500ml distilled water, as pH5.5 citrates (CPBS) cushioning liquid.
Embodiment 2
A kind of pH4.0 citrate (CPBS) piece, preparation method is as follows：
(1) it is 1.29 by weight:2.76:1:0.15 ratio, weighs citric acid, phosphoric acid hydrogen two respectively successively
If each dry weight of sodium, potassium chloride, cross-linked amylose, for example：Weigh citric acid 129g, disodium hydrogen phosphate
276g, potassium chloride 100g, cross-linked amylose 15g, matching is mixed evenly；
(2) tabletted on tablet press machine, pressure is 4 tons.Compacting 200, every weight 2.6g, containing lemon
Lemon acid, sodium dihydrogen phosphate, potassium chloride, cross-linked amylose, its part by weight are followed successively by
1.29:2.76:1:0.15。
In use, every is dissolved in 500ml distilled water, as pH4.0 citrates (CPBS) cushioning liquid.
Embodiment 3
A kind of pH5.0 citrate (CPBS) piece, preparation method is as follows：
(1) it is 3.6 by weight:2.7:1:0.2 ratio, weigh respectively successively citric acid, disodium hydrogen phosphate,
If each dry weight of potassium chloride, cross-linked amylose, for example：Citric acid 180g, disodium hydrogen phosphate 135g are weighed,
Potassium chloride 50g, cross-linked amylose 10g, matching is mixed evenly；
(2) tabletted on tablet press machine, pressure is 4 tons.Compacting 150, every weight 2.5g, containing lemon
Lemon acid, sodium dihydrogen phosphate, potassium chloride, cross-linked amylose, its part by weight are followed successively by 3.6:2.7:1:0.2.
In use, every is dissolved in 500ml distilled water, as pH5.0 citrates (CPBS) cushioning liquid.
Claims (6)
1. a kind of Citrate buffer piece, it is characterised in that：For circular, square, rhombus thin slice.
2. Citrate buffer piece according to claim 1, it is characterised in that：The composition of the thin slice
Composition is：Major ingredient, auxiliary material, adhesive, disintegrant.
3. Citrate buffer piece according to claim 2, it is characterised in that：The major ingredient is lemon
Acid；The auxiliary material is disodium hydrogen phosphate；The disintegrant is that cross-linked amylose, microcrystalline cellulose or crosslinking are poly-
Tie up ketone；Described adhesive is cross-linked amylose, microcrystalline cellulose, polyvinylpyrrolidone, saponified polyvinyl
Alcohol or amylopectin.
4. Citrate buffer piece according to claim 1, it is characterised in that：The thickness of the thin slice
For 1-10mm.
5. Citrate buffer piece according to claim 1, it is characterised in that：There is gas in the thin slice
Bubble.
6. the Citrate buffer piece described in a kind of claim 1, it is characterised in that：Step is as follows：
(1) purifying major ingredient, auxiliary material, adhesive, disintegrant, make to meet biological classes and grades in school, wherein major ingredient-citric acid,
Auxiliary material-disodium hydrogen phosphate, disintegrant-cross-linked amylose, microcrystalline cellulose, PVPP etc., adhesive
- cross-linked amylose, microcrystalline cellulose, polyvinylpyrrolidone, partly-hydrolysed polyvinyl alcohol, amylopectin
Deng；
(2) precise major ingredient, auxiliary material, adhesive, disintegrant；
(3) matching mixing, stirs；
(4) tablet press machine pressure is harmonized, and tabletting, pressure is 2-4 tons, is weighed, test passes, packaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510467866.8A CN106990801A (en) | 2016-01-20 | 2016-01-20 | Citrate buffer piece and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510467866.8A CN106990801A (en) | 2016-01-20 | 2016-01-20 | Citrate buffer piece and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106990801A true CN106990801A (en) | 2017-07-28 |
Family
ID=59413456
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510467866.8A Pending CN106990801A (en) | 2016-01-20 | 2016-01-20 | Citrate buffer piece and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106990801A (en) |
-
2016
- 2016-01-20 CN CN201510467866.8A patent/CN106990801A/en active Pending
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Legal Events
| Date | Code | Title | Description |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170728 |
|
| WD01 | Invention patent application deemed withdrawn after publication |