CN106990047A - Microwell plate detects anti-interference structure and micropore board checking device - Google Patents

Abstract

The invention discloses a kind of microwell plate detection anti-interference structure and micropore board checking device, microwell plate detects anti-interference structure, it is included in micropore cup axle to the joint unit with impulse stroke, the joint unit provides wall portion and vertical passage as formed by being surrounded wall portion, the top of the passage is used for the probe lower end for including micropore board checking device or the lower end for extension tube of popping one's head in, and during the lower dead center of joint unit arrival impulse stroke, the bottom of passage is used to include or the part for exposing plate face after the embedded microwell plate of micropore cup is included in part, when reaching the top dead centre of impulse stroke, passage is disengaged or disengaged just with micropore cup；Light sealing is formed between passage and probe or probe extension tube or light sealing structure is set in the periphery of joint unit.According to structure of the present invention is relatively easy and good shading effect.

Description

Microwell plate detects anti-interference structure and micropore board checking device

Technical field

The present invention relates to anti-dry for avoid that extraneous light disturbs during a kind of microwell plate detected object optical signal detecting Structure is disturbed, and configures the micropore board checking device of the anti-interference structure.

Background technology

The object of microwell plate detection is in micropore（It is general to be embedded in accommodating sample in micropore cup, micropore cup in micropore）Held The optical signal that sample is produced, reflects or marked is put, the optical signal is extremely faint, and extraneous light should be avoided to do micropore detection Disturb.Distribution of the micropore on microwell plate is about a line-column matrix, and the dimension for typically defining the line-column matrix is two sides of X, Y To dimension, to represent the direction of line-column matrix row and column.Definition simultaneously is Z-direction perpendicular to the dimension of line-column matrix plane.

On microwell plate micropore detection be typically one by one, the mode of progressively or column by column detected, using mode one by one as Main, such as Chinese patent literature CN201184885Y is to disclose a kind of detector detected one by one to micropore.But no matter It is the detection to single micropore or the detection of full line or permutation micropore, is required for shielding shadow of the extraneous light to object to be measured Ring.On the porous plate of current optical gauge measurement X, Y both direction motion during single hole, because detection object is extremely weak Optical signal, if the light entrance beyond gaging hole to be checked will have a strong impact on the accuracy of testing result.

Treat that light source beyond gaging hole includes ambient light and other non-treats the light that gaging hole is sent, it is however generally that, by certain Stroke Control in Z-direction, realize optical gauge relative to microwell plate move when do not produce interference, and shielding is treated in detection The requirement of source light beyond gaging hole.In some implementations, Z-direction optical gauge is relatively fixed, and microwell plate has the row Journey, microwell plate is provided in the stroke of Z-direction by cam mechanism.Because microwell plate is plate on the whole, cam is substantially more suitable for bar The driving of part, when driven object is plate, generally requires to set at least two cams on two camshafts, camshaft, synchronous Two camshafts are controlled, to realize linear motion of the plate in its normal direction, structure is relatively very complicated.

In some implementations, in given Z-direction stroke, detection bore portion is insinuated into the treating in gaging hole of microwell plate, according to By the engagement between hole wall, to eliminate the interference of extraneous light.Although the engagement between hole wall can meet the requirement of spuious light shield, It is understood, however, that the machining accuracy of micropore determines that all micropores can not possibly be attained by preferable size, equally Z-direction travel condition under, the engagement between foregoing hole wall may not meet given technical requirements, may exist engaged it is tight and The problem of causing to disengage difficult after engagement, it is also possible to because engagement is not enough, and the light barrier of effect can not be provided with.In addition, engagement Process often produces the friction between hole wall, and friction can then produce abrasion, if the abrasive dust produced by abrasion is mixed into testing sample, Very big noise can be produced.

Chinese patent literature CN106017539A proposes a kind of method that use cone carries out light-shielding, the document In point out how to operate the cone, and its cone is the protection to micropore collection, rather than to single micropore Protection, the method that the position relationship of cone and micropore how is controlled when being also short of micropore detection one by one.

Chinese patent literature CN103412117A then proposes a kind of shading ELISA Plate with base closure structures, bottom Close and light tight, to reduce the interference of bottom light, but do not consider the light pollution problem between top or adjacent cell.

The content of the invention

It is an object of the invention to provide a kind of structure is relatively easy and microwell plate of good shading effect detects anti-interference knot Structure, present invention also offers a kind of micropore board checking device that anti-interference structure is detected using the microwell plate.

According to embodiments of the invention there is provided a kind of microwell plate detect anti-interference structure, be included in micropore cup axle to The joint unit of impulse stroke, the joint unit provides wall portion and vertical passage as formed by being surrounded wall portion, the passage Top is used for the probe lower end for including micropore board checking device or the lower end for extension tube of popping one's head in, and joint unit reaches impulse stroke Lower dead center when, the bottom of passage is used to include or the part for exposing plate face after the embedded microwell plate of micropore cup is included in part, reaches During the top dead centre of impulse stroke, passage is disengaged or disengaged just with micropore cup；

Light sealing is formed between passage and probe or probe extension tube or light sealing structure is set in the periphery of joint unit.

Above-mentioned microwell plate detects anti-interference structure, alternatively has there is provided the structure of the impulse stroke：

First class formation, first class formation is configured to：The lower end of joint unit for conehead body have inclined side or The conical surface, so that the side or the conical surface are engaged with the upper end of micropore cup, and relative between microwell plate and micropore board checking device Horizontal movement is converted to the impulse stroke；

Second class formation, second class formation is configured to：In the side of joint unit provided with guiding continuous surface, for it is adjacent micro- The upper end of hole cup engages and horizontal movement relative between microwell plate and micropore board checking device is converted to the impulse stroke；Or

3rd class formation, the 3rd class formation is configured to：The drive device of above-below direction is configured for joint unit, it is described to provide Impulse stroke.

Alternatively, corresponding to the first class formation, passage lower end is configured to internal taper hole, and with medial slope or inner cone Face, the medial slope or inner conical surface are engaged with the upper end of current micropore cup, to provide joint unit and current micropore cup The stroke of disengagement；Or

The wall of passage lower end leaves spacing on the direction of horizontal movement between micropore cup margin of optic cup, to provide joint unit with working as When preceding micropore cup produces horizontal relative motions, the lower end of joint unit is disengaged with micropore cup upper end in the spacing range.

Alternatively, corresponding to the first class formation, when micropore cup reaches detecting position, the lower end surface of conehead body exceedes downwards Micropore cup margin of optic cup is engaged to the lower end surface of set a distance or conehead body with microwell plate plate face.

Alternatively, corresponding to the second class formation, the guiding continuous surface is sinusoidal corrugated.

Alternatively, respectively there is a sinusoidal corrugated corresponding to the both sides in the direction of horizontal movement in joint unit.

Alternatively, the drive device is cam mechanism, accordingly, and joint unit constitutes the driven structure of cam mechanism Part.

Alternatively, the camshaft of cam mechanism and the driving part of horizontal movement are synchronous.

Alternatively, it is that the joint unit configures an installing plate being fixed on micropore board checking device probe or main frame, The installing plate is provided with the via for passing through and being guided to joint unit for joint unit corresponding to the position of joint unit.

Alternatively, the coverage rate of installing plate in the horizontal direction in the orthogonal both direction of horizontal plane all be not less than 5 times Adjacent cells cup centre-to-centre spacing.

Alternatively, in being provided with calibration hole on installing plate, the calibration hole carries sealing cap.

Alternatively, the joint unit is external member, and the upper end of the external member has collar, the shaft shoulder that the collar is provided with it is micro- Hole cup is engaged when reaching detecting position with the upper face of installing plate.

Alternatively, the composition surface that the shaft shoulder is engaged with top panel is provided with optics labyrinth.

Alternatively, the light sealing structure that periphery is set is configured to sleeve piece, between the upper surface of the external member lower end and installing plate Form the first connected structure or be directly connected to, forming second between external member upper end and the seat of probe or the seat for extension tube of popping one's head in engages knot Structure；

First connected structure and the second connected structure are light sealing structure.

Alternatively, the upper and lower ends of external member are provided with close corresponding to the composition surface of the first connected structure and the second connected structure Seal.

Alternatively, the joint unit in the vertical direction is provided with the reset components for pushing joint unit.

Alternatively, the reset components are spring.

According to embodiments of the invention, a kind of microwell plate that anti-interference structure is detected equipped with foregoing microwell plate is additionally provided Detection means.

Above-mentioned micropore board checking device, alternatively, equipped with a frame, the peace for micropore board checking device host machine part Dress, and the guiding of above-below direction is provided for the joint unit.

Alternatively, the frame is the installing plate set parallel to microwell plate positioning table.

Alternatively, the probe of micropore board checking device is up big and down small variable-diameter structure；Or

For probe configuration probe extension tube, the probe extension tube and probe coaxial line, and the probe extension tube is up big and down small Variable-diameter structure.

According to embodiments of the invention, it is to be used for micropore cup and probe or probe extension tube pair to realize the sealed part of light The structure connect is joint unit, it is to be understood that relative to such as microwell plate, joint unit very little, and its manipulation is relatively easy, And it is sealed because its individual is small, is realized also relatively easy.Joint unit lower end includes micropore rim of a cup, light shield is formed, under Block extraneous light in end.And the blocking, it is necessary to the area blocked is also small of light is further carried out by light sealing structure in upper end, Realize that sealing is also relatively easy.

Brief description of the drawings

Fig. 1 is a kind of micropore board checking device trend of work schematic diagram in an embodiment（Equipped with the bullet resetted for cover Spring）.

Fig. 2 is micropore board checking device working state schematic representation in another embodiment（It is not equipped with the bullet resetted for cover Spring）.

Fig. 3 is micropore board checking device agent structure decomposing state schematic diagram in an embodiment.

Fig. 4 is the structural representation that covers in an embodiment.

Fig. 5 is probe or the structural representation of probe extension tube in an embodiment.

Fig. 6 is the type of drive structural representation that covers in an embodiment.

In figure：1. installing plate, 2. calibration holes, 3. calibration holes, 4. microwell plates, 5. micropores cup, 6., 7. bolts, 8. sealings Circle, 9. main frames, 10. probes or probe extension tube, 11. springs, 12. sealing rings, 13. sleeves, 14. sealing rings, 15. covers.

101. locating dowel.

151. shrinkage pools, 152. bellmouths, 153. optics labyrinths, 154. collars, 155. shoulders.

Embodiment

Fig. 1, Fig. 2 and Fig. 6 show a three-dimensional structure, are also the general identification method of mechanical field, generally, level Face is the plane on basis, typically using cartesian coordinate system（Plane right-angle coordinate）It is general to be represented just using X and Y to represent Two dimensions handed over.Left and right directions is left and right directions in figure, and Y is represented perpendicular to the direction of paper, and Z represents vertical direction, i.e., Above-below direction shown in figure.

Although in addition, tri- dimensions of X, Y, Z Z-direction in conventional application represents vertical direction, in more applications, Z-direction represents to determine plane to Y-direction with X（X/Y plane）Normal direction, therefore, if Z-direction be horizontal direction, X/Y plane should be perpendicular Face directly.It is understood that in the present invention, for convenience, using Z-direction as vertical direction, X/Y plane is horizontal plane, but simultaneously It is horizontal direction not influence Z-direction, and X/Y plane is the understanding of vertical plane.Meanwhile, for convenience of description, above-below direction is used in the present invention Z-direction is represented, but based on key element conversion, above-below direction therein can also be converted horizontally.

As shown in figure 1, microwell plate 4 is the flat board in horizontal plane, its upper surface is usually planar structure, and surface is opened from it Blind hole or the through hole through microwell plate 4, for being embedded in micropore cup 5.

It is understood that in most application, the upper surface part of the microwell plate 4 on 5 peripheries of micropore cup is divided into plane, Covered as shown in Figure 1 so as to use example 15 docking, cover 15 lower surface be planar end surface.

Because the propagation characteristic of light is linear transmission, although the problem of because of surface accuracy, plate face can produce diffusing reflection, When cover 15 has certain thickness, the engagement with reference to produced by of interplanar has relatively good inhibitory action to diffusing reflection.Can With understanding, cover 15 necessarily has certain thickness.

Simultaneously for the shielding of light, as shown in figure 1, the lower end of cover 15 does not include micropore cup 5 completely is embedded in microwell plate Expose the part of plate face after 4, be also the application to linear propagation of light attribute, especially under conditions of installing plate 1 is provided with, peace Filling plate 1 itself has the relatively good bridging effect to natural light, and the micropore cup 5 of including of 15 parts of cover is embedded in after microwell plate 4 Expose point of plate face, be mainly used in covering the interference of the current micropore cup 5 of 5 pairs adjacent of micropore cup.

Further, since micropore cup 5 is usually connection each other, it is however generally that, micropore cup 5 belongs to microwell plate 4 A part, the upper surface that the part for the connection of micropore cup 5 is provided is equivalent to the upper surface of microwell plate 4.

And then, it is to be understood that since constituting light shield, 15 are covered as shown in Figure 1, necessarily using shading material system Make.

The agent structure of structure shown in Fig. 1 and Fig. 2 is microwell plate detection anti-interference structure, i.e., to current micropore cup 5 , it is necessary to which the interference of covering outer light, extraneous light includes the light and room light produced by adjacent cells cup 5 when being detected Source and natural light.

Probe or probe extension tube 10 shown in Fig. 1 and Fig. 2 approach the rim of a cup of micropore cup 5 in detection, now pop one's head in Or there is a certain distance between probe extension tube 10 and micropore cup 5, interference light can influence extension tube 10 of popping one's head in or pop one's head in Normal detection.On the one hand, extraneous light is than stronger, by contrast, and the intensity of light is fainter produced by micropore cup 5, Directly produced light in micropore cup 5 is covered, it is impossible to complete detection.Document cited in background section is generally used to room The entirety of light and natural light produced by inner light source is blocked, and is short of the measure for shielding the interference of adjacent cells cup 5.

Relative to prior art, in the axial direction of micropore cup 5, that is, the Z-direction shown in figure, configuration has given working line The joint unit of journey, covers 15 as shown in Figure 1.

As shown in figure 1, in figure, the structure represented by label 9 is main frame, essence represents probe, in micropore board checking device In, the individual of usual probe is significantly larger than micropore cup 5, so that in most application, detection every time is containing many to one The detection of the micropore cup unit of individual micropore cup 5.The essence of label 10 is probe extension tube in Fig. 1, and probe extension tube is variable-diameter structure, Necking end is the lower end shown in figure, and its latus rectum is identical substantially with the bore of micropore cup 5.

For existing sonde configuration there is provided probe extension tube, the detection of single micropore cup 5 is adapted to.

In some applications, can specifically designed for single micropore cup 5 Individual Size, design with compared with low coverage spy Head, in other words, relative to conventional probe, adapts to the Individual Size of single micropore cup 5, popping one's head in itself can do relatively It is small.Preferably, using the structure shown in Fig. 1 as preferred structure, i.e., the probe extension tube of variable-diameter structure is configured on the basis of probe. In this structure, after variable-diameter structure is configured with, the large diameter portion on top directly constructs the masking to top light, more favorably In the configuration of sealing structure.

Micropore cup 5 is the test device with certain taper in the construction shown in fig. 1, and in the direction of motion of Z-direction On, based on drawing and forming is analogous to, micropore 5 glasss of footpath the best parts of cup are oral areas, and with equivalent nozzle structure.It is based on This, it is to be understood that joint unit is equivalent to the docking to pipe fitting.For the technical staff of mechanical field, pipe fitting pair Connect, connection, docking or connection can be docked or connected by conventional pipe joint, can also pass through reducing Structure is docked or connected.Therefore, in certain embodiments, probe can use the relatively large spy of conventional visual field Head, is realized by reducing and is docked with single micropore glasss 5, simply corresponding joint unit individual is larger.

And then, therefore the gabarit of probe or probe extension tube 10, can so manage it can be appreciated that be cylinder accordingly Docking between solution, probe or probe extension tube 10 and micropore cup 5, with covering outer light, equivalent to the docking of pipe fitting, because This, joint unit can be understood as tubular body, and the cross section of the tubular body is preferably circle, but is not excluded for other cross-sectional configurations Tubular body.

Joint unit provides wall portion, for covering outer light, it was found from its inventive concept, joint unit necessarily shading Material is constituted.And passage vertical as formed by being surrounded wall portion then provides probe or probe extension tube 10 detects micropore cup 5.

And then, on the passage, it should construct the sampling of the optical signal suitable for straightline propagation first, therefore, and this leads to Road should at least include straight-through part.

The top of the passage is used for the probe lower end for including micropore board checking device or the lower end for extension tube of popping one's head in.Include Contact can be produced, can also be disengaged each other, in the structure shown in Fig. 1 and Fig. 2, cover 15 is contacted with micropore glass 5, can be with Partly including under conditions of micropore cup 5 exposes the part of plate face, still there is relatively good light sealing effectiveness.Cover 15 is in Fig. 1 With in the structure shown in Fig. 2 not with pop one's head in or probe extension tube 10 contact.Wherein, in certain embodiments, the lower end of passage The cover 15 that the structure of generation light-shielding is mainly for example shown in Fig. 1 relies on 15 self gravitations of cover and spring with microwell plate 4 11 normal pressure realizes that light-shielding is realized in aspectant tight engagement, and with reference to Fig. 2, then the gravity for relying only on cover 15 realizes phase To tight engagement.In certain embodiments, as it was previously stated, can use what the inwall of cover 15 and the margin of optic cup of micropore cup 5 be engaged Mode realizes optical encapsulant.

In some applications, as shown in figure 1, the essence of main frame 9 is also carried on seat 6, seat 6 is parallel to each other with installing plate 1, seat The distance between 6 and installing plate 1 can be changes, and bolt 7 has polished rod and screw rod, wherein threaded shank as illustrated in the drawing Divide and be fixedly connected with installing plate 1, smooth bar part, which may be constructed, convinces hole by patient analysis on guide pillar, seat 6, bullet is installed between seat 6 and installing plate 1 The gravity of institute's installing component is mainly carried by installing plate 1 on spring, main frame 9 and seat 6, and remaining part can be for example, by cover 15 To carry, on this condition, cover 15 can provide the masking that end joined realizes extraneous light, than the cover 15 shown in figure slightly It is long, and cover 15 upper end and can fix on present 6, and the impulse stroke of cover 15 is produced with moving up and down for seat 6, and it is fixed The formed masking structure of connection is relatively good more than the effect of engagement.

In certain embodiments, cover 15 upper end and Fig. 1 shown in seat 6 disengage, cover 15 inwall with for example shown in Fig. 1 Probe or pop one's head in extension tube 10 outer wall slide light sealing coordinate.

In addition, as shown in figure 1, visible in Fig. 1, sleeve 13 is additionally provided with the outside of cover 15, can as preferred embodiment With the light shield of outer layer, preferably shaded effect can be obtained.

And then, sliding light sealing cooperation is then used to coordinate to have to move up and down（Z-direction as shown in Figure 1）Movable part Part, i.e., foregoing joint unit moves up and down the auxiliary for detecting state and the transposition state of micropore cup 5.Specifically, Fig. 1 can See, the upper surface for exposing microwell plate 4 of the part of micropore cup 5, in favor of the abjection of micropore cup 5.The position between micropore cup 5 is carried out again When putting conversion, interference can be produced by protruding from the part of the upper surface of microwell plate 4, it is therefore desirable to which the joint unit moved up and down is to avoid Interference, or only exist the interference of friction class.

In Z-direction, impulse stroke is determined by top dead centre and lower dead center, and lower dead center in certain embodiments can directly by The upper surface of microwell plate 4 is constrained, in certain embodiments, can by adjacent cells cup 5 margin of optic cup support and realize it is spacing, In certain embodiments, can be supported by the margin of optic cup of current micropore cup 5 and realize it is spacing, and in further embodiments, can Spacing, as this kind of structure in such as Fig. 1 and Fig. 2 is realized with support or additional support by the upper surface of installing plate 1.

The control of lower dead center is fairly simple, and the stop for belonging to microwell plate 4 in some embodiments is spacing, and in other implementations In example, then it can be realized using the stop of adjacent cells cup spacing.Top dead centre only considers the space requirement of Z-direction, it is clear that working line Cheng Yue great, the space requirement to Z-direction is also higher, to avoid interference, or only exists the interference of friction class, as shown in fig. 1 Cover 15, its lower end avoids the part that micropore cup 5 protrudes from microwell plate 4, it is too high then can irrational increase Z-direction sky Between.

On impulse stroke, a kind of condition is autonomous control, and the behaviour to joint unit is realized using the profile of micropore cup 5 Control, as shown in figure 4, the lower end of cover 15 has conical head 152 in figure, is sideways inclined inclined-plane or the conical surface, utilizes the inclined-plane Or the cooperation of the conical surface and the margin of optic cup of micropore cup 5, horizontal movement can be changed into vertical motion.Another condition is passive control System, i.e., the drive device that configuration driven cover 15 is moved up and down.

In certain embodiments, so joint unit reach impulse stroke lower dead center when, now, its lower end is pressed in micropore The upper surface of plate 4, the bottom of passage is used to include after micropore cup 5 is embedded in microwell plate 4 and exposes the part of plate face and reach working line During the top dead centre of journey, passage is disengaged or disengaged just with micropore cup 5.

Wherein, foregoing lucky disengagement is represented in Z-direction, i.e., vertical direction is disengaged, and is not offered as disengagement physically, as before It is described, on this condition, the state that the margin of optic cup upper end in micropore cup 5 is engaged with the lower end of cover 15.

Disengaged, represent impulse stroke it is up during, just generated such as micropore cup 5 with cover 15 it is complete It is complete to disengage.

As it was previously stated, joint unit can be fixedly connected with extension tube 10 of popping one's head in or pop one's head in as illustrated in the drawing, can also It is flexibly connected, under the conditions of being fixedly connected, light is sealedly realized relatively easily, will not be repeated here.Under the conditions of flexible connection, because needing Reserve drive gap, light sealedly realizes, but just straightline propagation, masking to light more complex relative to being fixedly connected First have to avoid light from being directly shining into micropore cup 5, then consider further that the interference produced by diffusing reflection, diffusing reflection light intensity is relatively small, And it is big to be sealingly engaged face, it is possible to shielding completely.

Below for the forms of motion of joint unit, and joint unit forms of motion working method to related sealing It is described as follows：

In the structure shown in Fig. 4, the external diameter of cover 15 is a variable-diameter structure, merely for its gabarit, can be simply geographical Solve as a multi-diameter shaft, wherein be topmost maximum diameter, collar 154 as shown in Figure 4, then in continued access path portion master Body, bottom is a conical head 152, and conical head 152 can be pyramidal structure, preferably positive rectangular pyramid, the four of positive rectangular pyramid Individual side X-axis to that indicated in the drawings and Z axis are adapted.Conical head 152 can also be that there is provided the conical surface, circular cone noodles for conical structure Under part, it is not necessary to consider side and X-axis or Z axis whether just to the problem of.

And then, the side of conical head 152 or the conical surface are engaged with the upper end of micropore cup 5, when cover 15 is traversing or during vertical shift, Such as inclined side and the upper end of micropore cup 5 form Wedge mechanism, so as to which vertically, i.e., traversing or vertical shift is converted into The motion of Z-direction in figure, so as to provide the impulse stroke.

It is understood that on this condition, the power of foregoing Wedge mechanism is detected from microwell plate 4 with microwell plate to be filled Relative horizontal movement conversion between putting.

Also, it is understood that, because current micropore cup 5 finally needs to bring the bottom of passage into, but also can From abjection, therefore, the power resources that passage includes micropore cup 5 are mainly current micropore cup 5, and dynamic when deviating from from passage Power source is also that as shown in Figure 1, cover 15 is when including current micropore cup 5, the inclined-plane of shrinkage pool 151 and micropore from micropore cup 5 Wedge effect equally occurs for cup 5, makes cover 15 along Z-direction lifting, promotes current micropore cup 5 to depart from cover 15, next to treat micrometer Hole cup is initially entered.When producing relative horizontal movement so as to microwell plate 4 and micropore board checking device, adjacent micropore cup 5 with Conical head 152 can form Wedge mechanism.

In certain embodiments, current micropore cup 5 imports or exported the current micropore cup 5 that places one's entire reliance upon below cover 15 With the cooperation of cover 15, two Wedge mechanisms, structure as shown in Figure 4, the lower end Inner Constitution outer inclined-plane of cover 15, outside structure are formed Into outer inclined-plane.

The margin of optic cup of micropore cup 5 is run to after Beveled ends, and cover 15 loses support and fallen, and by current micropore glass 5 Cover.

On this condition, upper end sealing uses dynamic sealing as far as possible, and the frictional force of dynamic sealing should be as small as possible, figure There is provided sealed for frictional force of the frictional force that cover 15 moves up and down in 1 between the via that it opens cover 15 with installing plate 1 It is extra part, i.e., the sleeve 13 shown in figure belongs to static seal, on this condition, the frictional force suffered by cover 15 is very It is small.

Collar 154 shown in Fig. 4, on the one hand provides spacing, the another aspect moved downward of cover 15, is also used for masking Cover 15 and the gap of 1 perforate of installing plate.

Further, optics labyrinth is also provided with the downward end face of the collar 154 shown in Fig. 4, to improve the screening to light Gear ability, can especially effectively eliminate diffusing reflection.

And then, match it is foregoing with conical head 152 for a component of Wedge mechanism under conditions of, passage lower end is configured to Internal taper hole, shrinkage pool 151 as shown in Figure 4, shrinkage pool 151 provides medial slope or inner conical surface, when the medial slope or interior When the conical surface engage with the upper end of current micropore cup 5, the oblique of joint unit and the margin of optic cup disengagement of current micropore glass 5 can be provided Wedge mechanism.

Understood based on foregoing structure, either inner conical surface or male cone (strobilus masculinus), due to being required for the margin of optic cup with micropore cup 5 to connect Close, therefore, in certain embodiments, the height of such as conical head 152 necessarily be greater than the part that micropore cup 5 exposes microwell plate 4 Highly.

In addition, it is contemplated that the forms of motion of Wedge mechanism, determining needs stroke to change one's profession journey, therefore, is used as necessary condition One of, the wall of passage lower end leaves spacing on the direction of horizontal movement between micropore 5 margins of optic cup of cup, with provide joint unit with When current micropore cup 5 produces horizontal relative motions, the lower end of joint unit takes off with 5 upper ends of micropore cup in the spacing range Open.

Correspondingly, in certain embodiments, when current micropore cup 5 reaches detecting position, conical head 152 and adjacent cells The upper end of cup 5 keeps contact, and the lower end surface of conical head 152 is engaged with the plate face of microwell plate 4, forms lower end sealing.

On the construction of Wedge mechanism, the end face structure of joint unit itself can not be directly used, but by docking section The other parts of part are provided, such as in the side of joint unit provided with guiding continuous surface, for the upper termination with adjacent cells cup 5 Close and horizontal movement relative between microwell plate 4 and micropore board checking device is converted to the impulse stroke.

Guide continuous surface and be different from structure as shown in Figure 1, it is not necessary to rely on end structure, it is not necessary to consider that abrasion is produced Leakage problem.

Especially, the guiding continuous surface that side is set can have multiple flex points, so as to realize the rim of a cup of micropore cup 5 The continuous control for entering, deviating from from such as cover 15, the design to the self structure of cover 15 is relatively easy.

It is preferably sinusoidal corrugated on guiding continuous surface.

As further preferred, respectively there is a sine wave corresponding to the both sides in the direction of horizontal movement in joint unit Face, so that joint unit smoothly can be raised or fallen.

For the driving of joint unit, independent drive device can also be provided for joint unit, as shown in fig. 6, being figure Shown in cover 15 provide ratchet mechanism, cover 15 is driven by the ratchet 16 of ratchet mechanism, cover 15 is moved up and down.

Ratchet mechanism belongs to one kind of batch (-type) mechanism, and the ratchet of ratchet 16 is sequentially engaged with shoulder 155, realizes cover 15 Drive up and down.

In certain embodiments, can be using one of most typical mechanical mechanism, i.e. cam machine to the driving of shoulder 155 The cam of structure drives.

Accordingly, joint unit constitutes the driven member of cam mechanism, and the cam of cam mechanism is than occupied by ratchet 16 Space it is small, beneficial in detection means arrange.

Further, the camshaft of cam mechanism and the driving part of horizontal movement are synchronous, so as to be calculated by associating, make Joint unit movement in Z-direction with X to or the motion of Y-direction be adapted.

In a preferred embodiment, it is that micropore board checking device probe or main frame 9 are fixed in the joint unit configuration one On installing plate 1, the installing plate 1 corresponding to joint unit position be provided with is passed through for joint unit and to joint unit guide Via.

Specifically, as shown in figure 1, in figure cover 15 pass through it is 5 relative with corresponding micropore cup after installing plate 1.The via is made For the guide hole of cover 15, closed so that producing dynamic connection between probe or probe extension tube 10 that cover 15 need not be to that indicated in the drawings System.

The micropore cup 5 that installing plate 1 is covered to it can also play certain bridging effect, weaken and enter installing plate Light intensity between 1 and microwell plate 4, alleviates difficulty of the plane engagement to shield light between cover 15 and microwell plate 4.

And then, it is contemplated that the shielding to light, the coverage rate of installing plate 1 in the horizontal direction is in orthogonal two of horizontal plane Adjacent cells cup centre-to-centre spacing on direction all not less than 5 times.

In addition, as shown in Fig. 1 and 2, during detection, probe or probe extension tube 10 and micropore cup the distance between 5 are not Greatly, it is adaptable, the impulse stroke also very little of cover 15, therefore, installing plate 1 and the inevitable very little of the distance between microwell plate 4, in water The installing plate 1 with larger dead area is conducive to obtaining relatively good screening effect in plane.

On the relative motion between microwell plate 4 and such as installing plate 1, micropore cup 5 is realized by the given step pitch of setting Detect one by one.Error can also be produced by automatically controlling, the axiality of the probe that can make or probe extension tube 10 and current micropore cup 5 by It is cumulative big.Blocked by installing plate 1, operating personnel are not easy to see the situation of axiality change.

In a preferred embodiment, in being provided with calibration hole 4 on installing plate 1, calibration hole 4 is provided with a pair, in two calibration holes 4 The heart can calibrate alignment degree between installing plate 1 and microwell plate 4 away from the integral multiple for adjacent cells centre-to-centre spacing through calibration hole.

And then, calibration hole 4 carries sealing cap, only in calibration, opens sealing cap, in the detection of micropore cup 5, puts on envelope Cap, for shutting out the light.

On the joint unit, preferably external member, in the structure shown in Fig. 4, joint unit is about an axle sleeve Structure.

The upper end of above-mentioned external member has collar 154, when the shaft shoulder that the collar 154 is provided reaches detecting position with micropore cup 5 Engaged with the upper face of installing plate 1.The interior tube body of external member is foregoing passage.

In preferred embodiment, the light sealing structure that periphery is set is configured to sleeve piece, sleeve 13 as shown in Figure 1, The first connected structure is formed between the external member lower end and the upper surface of installing plate 1 or is directly connected to, external member upper end with probe seat 6 or The second connected structure is formed between the seat for extension tube of popping one's head in.

Fig. 3 is visible, and the upper and lower ends of sleeve 13 are provided with sealing ring 12, the locating slot of sealing ring 14, and up and down the two of sleeve 13 Not there is attachment structure in end, and simply engage with probe or probe extension tube 10 and installing plate 1.

In certain embodiments, the upper end of sleeve 13 can be fixedly connected with extension tube 10 of popping one's head in or pop one's head in.

As it was previously stated, the engagement of end face and plate face can meet required light seal request, therefore, the upper end of sleeve 13 and Lower end can rely solely on end joined and realize that light is sealed, and on this condition, corresponding composition surface is needed with relatively good Flatness.

In foregoing structure, light sealing structure is fitted with sealing ring 12 or sealing ring 14, relatively reliable close to realize Envelope.

In the structure shown in Fig. 1 and Fig. 2, it is fixedly connected between seat 6 and installing plate 1 by bolt 7, passes through the pre- of bolt 7 Clamp force makes the deformation of sealing ring 12 and sealing ring 14, obtains relatively reliable sealing.

Shown in Fig. 1 and Fig. 2, it is distinguished to essentially consist in Fig. 1 to contain in spring 11, Fig. 2 and only realized using the gravity for covering 15 Cover 15 is pushed.

Wherein, spring 11 provides pushing for such as cover 15, so that the sealing between the lower end of cover 15 and microwell plate 4 more may be used Lean on.

The upper end of cover 15 can configure gum cover, to provide lower pressure.

In an embodiment of the present invention, it is preferred that emphasis is shading when micropore cup 5 is detected, for its of micropore board checking device He can use conventional structure in part.In addition, on the probe shown in Fig. 1 or probe extension tube 10, as it was previously stated, can With equipped for existing micropore board checking device.

In Fig. 1, the upper end of seat 6 is used to carry main frame 9, and the upper end of seat 6 is provided with the locating slot of sealing ring 8, main frame 9 and seat 6 Sealing is realized in docking.

It is not fixedly connected between probe extension tube and main frame 9, the extension of probe is realized by way of undertaking, probe prolongs Long tube adapts to the size of micropore cup 5.Main frame 9 shown in figure is used to represent existing probe.

Installing plate 1 shown in Fig. 1 constitutes frame, the installation for micropore board checking device host machine part.

Claims (21)

1. a kind of microwell plate detects anti-interference structure, it is characterised in that be included in micropore cup axle to the docking with impulse stroke Part, the joint unit provides wall portion and vertical passage as formed by being surrounded wall portion, and the top of the passage is used to include micro- The probe lower end of orifice plate detection means or the lower end for extension tube of popping one's head in, and during the lower dead center of joint unit arrival impulse stroke, lead to The bottom in road exposes the part of plate face for including or partly including after the embedded microwell plate of micropore cup, reach the upper of impulse stroke and stop During point, passage is disengaged or disengaged just with micropore cup；

Light sealing is formed between passage and probe or probe extension tube or light sealing structure is set in the periphery of joint unit.

2. microwell plate according to claim 1 detects anti-interference structure, it is characterised in that provide the knot of the impulse stroke Structure has：

First class formation, first class formation is configured to：The lower end of joint unit for conehead body have inclined side or The conical surface, so that the side or the conical surface are engaged with the upper end of micropore cup, and relative between microwell plate and micropore board checking device Horizontal movement is converted to the impulse stroke；Or

Second class formation, second class formation is configured to：In the side of joint unit provided with guiding continuous surface, for it is adjacent micro- The upper end of hole cup engages and horizontal movement relative between microwell plate and micropore board checking device is converted to the impulse stroke；Or

3rd class formation, the 3rd class formation is configured to：The drive device of above-below direction is configured for joint unit, it is described to provide Impulse stroke.

3. microwell plate according to claim 2 detects anti-interference structure, it is characterised in that corresponding to the first class formation, lead to Road lower end is configured to internal taper hole, and has medial slope or inner conical surface, the medial slope or inner conical surface and current micropore The upper end engagement of cup, to provide the stroke that joint unit is disengaged with current micropore cup；Or

The wall of passage lower end leaves spacing on the direction of horizontal movement between micropore cup margin of optic cup, to provide joint unit with working as When preceding micropore cup produces horizontal relative motions, the lower end of joint unit is disengaged with micropore cup upper end in the spacing range.

4. microwell plate according to claim 3 detects anti-interference structure, it is characterised in that corresponding to the first class formation, When micropore cup reaches detecting position, the lower end surface of conehead body exceedes downwards lower end end of the micropore cup margin of optic cup to set a distance or conehead body Face is engaged with microwell plate plate face.

5. microwell plate according to claim 2 detects anti-interference structure, it is characterised in that corresponding to the second class formation, institute It is sinusoidal corrugated to state guiding continuous surface.

6. microwell plate according to claim 5 detects anti-interference structure, it is characterised in that correspond to level in joint unit The both sides in the direction of motion respectively have a sinusoidal corrugated.

7. microwell plate according to claim 2 detects anti-interference structure, it is characterised in that the drive device is cam machine Structure, accordingly, joint unit constitute the driven member of cam mechanism.

8. microwell plate according to claim 7 detects anti-interference structure, it is characterised in that the camshaft and water of cam mechanism The driving part of flat motion is synchronous.

9. anti-interference structure is detected according to any described microwell plate of claim 1 ~ 8, it is characterised in that be the joint unit The installing plate that configuration one is fixed on micropore board checking device probe or main frame, the installing plate is opened corresponding to the position of joint unit There is the via for passing through and being guided to joint unit for joint unit.

10. microwell plate according to claim 9 detects anti-interference structure, it is characterised in that installing plate is in the horizontal direction Coverage rate in the orthogonal both direction of horizontal plane all not less than 5 times adjacent cells cup centre-to-centre spacing.

11. microwell plate according to claim 10 detects anti-interference structure, it is characterised in that in being provided with calibration on installing plate Hole, the calibration hole carries sealing cap.

12. microwell plate according to claim 9 detects anti-interference structure, it is characterised in that the joint unit is external member, The upper end of the external member has collar, is connect when the shaft shoulder that the collar is provided and micropore cup reach detecting position with the upper face of installing plate Close.

13. microwell plate according to claim 12 detects anti-interference structure, it is characterised in that the shaft shoulder is engaged with top panel Composition surface is provided with optics labyrinth.

14. microwell plate according to claim 9 detects anti-interference structure, it is characterised in that the light sealing knot that periphery is set Structure is configured to sleeve piece, and the first connected structure is formed between the upper surface of the external member lower end and installing plate or is directly connected to, in external member The second connected structure is formed between the seat of the seat or extension tube of popping one's head in of end and probe；

First connected structure and the second connected structure are light sealing structure.

15. microwell plate according to claim 14 detects anti-interference structure, it is characterised in that the upper and lower ends of external member are corresponding Sealing ring is provided with the composition surface of the first connected structure and the second connected structure.

16. anti-interference structure is detected according to any described microwell plate of claim 1 ~ 8, it is characterised in that the joint unit In the vertical direction is provided with the reset components for pushing joint unit.

17. microwell plate according to claim 16 detects anti-interference structure, it is characterised in that the reset components are bullet Spring.

18. one kind detects the micropore board checking device of anti-interference structure with described microwell plate any just like claim 1 ~ 17.

19. micropore board checking device according to claim 18, it is characterised in that equipped with a frame, for microwell plate inspection The installation of device host machine part is surveyed, and the guiding of above-below direction is provided for the joint unit.

20. micropore board checking device according to claim 19, it is characterised in that the frame is fixed parallel to microwell plate The installing plate that position workbench is set.

21. according to any described micropore board checking device of claim 18 ~ 20, it is characterised in that micropore board checking device Pop one's head in as up big and down small variable-diameter structure；Or

For probe configuration probe extension tube, the probe extension tube and probe coaxial line, and the probe extension tube is up big and down small Variable-diameter structure.