CN106984368A - A kind of hepatitis B controlled based on pump valve examines micro-fluidic chip and analysis method soon - Google Patents
A kind of hepatitis B controlled based on pump valve examines micro-fluidic chip and analysis method soon Download PDFInfo
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- CN106984368A CN106984368A CN201710206116.4A CN201710206116A CN106984368A CN 106984368 A CN106984368 A CN 106984368A CN 201710206116 A CN201710206116 A CN 201710206116A CN 106984368 A CN106984368 A CN 106984368A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/576—Immunoassay; Biospecific binding assay; Materials therefor for hepatitis
- G01N33/5761—Hepatitis B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/08—Regulating or influencing the flow resistance
- B01L2400/082—Active control of flow resistance, e.g. flow controllers
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Abstract
Micro-fluidic chip and analysis method are examined soon the invention discloses a kind of hepatitis B controlled based on pump valve, it is characterised in that：Four reagent inlets, a sample introduction entrance.Reagent inlet and sample holes are connected by fluid channel with reaction tank；Described micro-fluidic chip is made up of egative film, substrate and cover plate, and cover plate and egative film use PS materials, and substrate material can be the macromolecule polymer materials such as quartz, glass.Reaction tank is connected by fluid channel with waste liquid pool.Four reagent inlets connect enzyme standard liquid bottle, Washing liquid bottle, the substrate solution bottle and terminate liquid bottle of outside valve unit respectively.The fluid channel for connecting cleaning solution reagent bottle is located at the lower section of substrate, and other fluid channels are located at the top of substrate.Reagent is pumped in reaction tank by micro-fluidic chip external connection valve unit, completes the quick detection of hepatitis B.Microfluidic chip structure that the present invention is designed is simple, with low cost, small volume, detection method have the advantages that rapidly and efficiently, reliability is high, be easy to implement Automated condtrol.
Description
Technical field
The present invention relates to micro-fluidic chip (micro-fluidic chip) and field of medical applications.It is more particularly to a kind of
The hepatitis B controlled based on pump valve examines micro-fluidic chip and analysis method soon.
Background technology
Hepatitis B is global disease, the whole world about 300,000,000 hepatitis type B viruses (HBV) carrier at present, and China is even more
High incidence of hepatitis b, possesses 0.93 hundred million hepatitis B carriers, wherein hepatitis B patient about 30,000,000.HBV indicates quality testing
Survey is the hepatitis B infected important evidence of clinical diagnosis, and clinically hepatitis B detection mainly uses traditional blood testing, most
It is conventional for ELISA (enzyme linked immunosorbent assay write a Chinese character in simplified form ELISA), i.e., based on antigen-anti-
A kind of analysis method of body specific binding, with high sensitivity and specificity, but common immunoassays brooding time is longer,
Instrument cost height, operation sequence is complicated, and sample consumption is big, and generally requires to be equipped with the operating environment of laboratory level and special
The technical operation personnel of industry, it is impossible to quick detection in real time, it is difficult to meet the requirement of field test, disadvantages mentioned above is then seriously hindered
Hepatitis B is instant, the application and popularization of quick detection.Therefore, a kind of quick, portable, with low cost blood testing dress is developed
Put and be significant.
Compared with conventional microplates immunoassay, micro-fluidic chip immunoassay has the advantage that：1. it is easy to operate, instrument
Device is miniaturized；2. immune response is carried out in microchannel, and specific surface area is big, and diffusion length significantly shortens, and accelerates antigen-antibody
Reaction, shortens the reaction time；3. small volume, saves reagent and sample；4. can multisample, multi objective detect simultaneously.Therefore, it is micro-
Pump valve formula fluidic chip immunoassay has a wide range of applications in blood safety analysis detection field application.
The content of the invention
The present invention is intended to provide a kind of quick, portable, automatic, high integration micro-fluidic chip dress driven based on pump valve
Put, and realize and use ELISA method, the detection to hepatitis B.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of hepatitis B controlled based on pump valve examines micro-fluidic chip soon, and the micro-fluidic chip is provided with reaction tank, waste liquid
Pond, fluid channel, sample holes, steam vent and the reagent inlet for connecting injection device, micro-fluidic chip is by egative film, substrate and cover plate
Composition.Wherein：The reaction tank of micro-fluidic chip is connected by fluid channel with four reagent inlets, and reagent inlet branch is in reaction tank
Surrounding, reaction tank is connected by fluid channel with waste liquid pool, and the fluid channel of connection cleaning solution reagent bottle is located at the lower section of substrate.Even
The fluid channel for connecing enzyme standard liquid reagent bottle, substrate solution bottle and terminate liquid bottle is located at the top of substrate.
Four reagent inlets are attached with outside valve unit.Corresponding reagent bottle is connected respectively：Enzyme marking reagent bottle,
Cleaning solution reagent bottle, AB substrate solutions reagent bottle and terminate liquid reagent bottle, make corresponding reagent be reacted into reaction tank.
In preferred embodiment, cover plate and egative film use PS materials, substrate material can be quartz, glass, PDMS,
The macromolecule polymer materials such as PMMA or PC.Coverslip thickness is 2mm, and egative film thickness is 1mm, and substrate thickness is 4mm.
In preferred embodiment, the volume of reaction tank is 150cm3。
In preferred embodiment, the width of fluid channel is 400 μm.
In preferred embodiment, the reagent inlet 2 of cleaning solution reagent bottle is connected, positioned at the left of reaction tank, sample holes
Positioned at the upper right side of reaction tank, steam vent is located at the center of waste liquid pool.
In preferred embodiment, the fluid channel of connection cleaning solution reagent bottle is located at the lower section of substrate.Ligase standard liquid is tried
The fluid channel of agent bottle, substrate solution bottle and terminate liquid bottle is located at the top of substrate.
A kind of hepatitis B controlled based on pump valve examines micro-fluidic chip and analysis method soon, and implementing hepatitis B detection includes following step
Suddenly：
1) egative film, substrate and the cover plate of the micro-fluidic chip of foregoing hepatitis B quick detection are made, region pair is coated with egative film
Antibody is coated with, and 37 DEG C incubate 2~3 hours.Then three layers of progress alignment bonding of chip；
2) start external valve unit, cleaning solution is entered reagent inlet 2, rinse reaction tank, be again started up pump valve dress
Put and admit air into reagent inlet 2, be that the cleaning solution in reaction tank enters waste liquid pool, and reaction tank is dried up.
3) by sample holes, serum is injected, serum enters after reaction tank, the sealing of parallel sample holes, fully 10 points of hatching
Clock；
4) start external valve unit, admit air into reagent inlet 2, the serum in reaction tank is flowed into waste liquid pool；
5) start external valve unit, cleaning solution is entered reagent inlet 2, rinse reaction tank, be again started up pump valve dress
Put and admit air into reagent inlet 2, be that the cleaning solution in reaction tank enters waste liquid pool, and reaction tank is dried up；
6) by external valve unit, enzyme standard liquid is made to enter reagent inlet 1, enzyme standard liquid enters after reaction tank, resisted
Antigen-antibody reaction；
7) start external valve unit, admit air into reagent inlet 2, the enzyme standard liquid in reaction tank is flowed into waste liquid
Pond；
8) start external valve unit, cleaning solution is entered reagent inlet 1, rinse reaction tank, be again started up pump valve dress
Put and admit air into reagent inlet 1, the cleaning solution in reaction tank is entered waste liquid pool, and dried up to reaction tank；Repeat this
Step 5 time；
9) by external valve unit, AB substrate solutions are made to enter reagent inlet 3, and developed the color instead into reaction tank progress
Should；
10) by external valve unit, terminate liquid is entered reagent inlet 4, and terminating reaction carried out into reaction tank,
Visually visible Preliminary detection result；
11) absorbance detection is carried out to the reagent in reaction tank with ELIASA, draws final result.
In preferred embodiment, specific step is as follows：
1) egative film, substrate and the cover plate of the micro-fluidic chip of foregoing hepatitis B quick detection are made, region pair is coated with egative film
Antibody is coated with, and 37 DEG C incubate 2~3 hours.Then three layers of progress alignment bonding of chip；
2) start external valve unit, 160ml cleaning solutions is entered reagent inlet 2, rinse reaction tank, be again started up pump
Valve gear admits air into reagent inlet 2, is that the cleaning solution in reaction tank enters waste liquid pool, and reaction tank is dried up.
3) by sample holes, 40ml serum is injected, serum enters after reaction tank, the sealing of parallel sample holes, fully hatching
10 minutes；
4) start external valve unit, admit air into reagent inlet 2, the serum in reaction tank is flowed into waste liquid pool；
5) start external valve unit, 160ml cleaning solutions is entered reagent inlet 2, rinse reaction tank, be again started up pump
Valve gear admits air into reagent inlet 2, is that the cleaning solution in reaction tank enters waste liquid pool, and reaction tank is dried up；
6) by external valve unit, 40ml enzymes standard liquid is made to enter reagent inlet 1, enzyme standard liquid enters after reaction tank, entered
Row antigen-antibody reaction, 37 DEG C of incubations；
7) start external valve unit, admit air into reagent inlet 2, the enzyme standard liquid in reaction tank is flowed into waste liquid
Pond；
8) start external valve unit, 160ml cleaning solutions is entered reagent inlet 2, rinse reaction tank, be again started up pump
Valve gear admits air into reagent inlet 2, the cleaning solution in reaction tank is entered waste liquid pool, and dried up to reaction tank；Weight
Multiple this step 5 time；
9) by external valve unit, 120mlAB substrate solutions are made to enter reagent inlet 3, and shown into reaction tank
Colour response；
10) by external valve unit, 40ml terminate liquids are made to enter reagent inlet 4, and terminated into reaction tank
Reaction, the visible Preliminary detection result of naked eyes；
11) absorbance detection is carried out to the reagent in reaction tank with ELIASA, draws final result.
Compared with existing method and device, the advantage of the invention is that：
1) present invention can accurately control the amount of liquid using miniature valve unit as driving force, its chip and its
Design device relatively simple, operate more convenient.And the successful of washing process can be greatly improved, makes what ELISA was tested
Final detection result is more accurate.
2) ELISA courses of reaction key reaction of the invention is easier to realize coating in the lower floor of reaction tank.
3) micro-fluidic chip of the present invention and its analysis method have that simple in construction, sample consumption is small, detect into
The advantages of this is low, detection speed is fast.
4) present invention is simple to operate, and in addition to sample to be manually added and sealing sample holes, remaining only needs to valve unit
It is automatic to carry out, disposable real-time, the portable detection of hepatitis B can be detected.
Brief description of the drawings
Fig. 1 and Fig. 2 are according to the pump valve formula micro fluidic chip unit of the present invention for blood hepatitis B quick detection
Structure chart
Fig. 1 is the plan for showing chip internal structure.
Wherein, 1,2,3 and 4 be reagent inlet, respectively by flexible pipe connect the enzyme standard liquid bottle of pump valve structure, Washing liquid bottle,
AB substrate solutions bottle and terminate liquid bottle.5 be sample holes.1', 2', 3', 4', 5' and 6 are fluid channel, and 7 be reaction tank, and 8 be waste liquid
Pond.1', 3', 4', 5' and 6 fluid channels are located at the top of substrate, and 2' fluid channels are located at the lower section of substrate.
Fig. 2 is the stereogram for showing chip three-decker.
Its mediella C dash area is antigen or antibody coating area.
Fig. 3 is the sample introduction process schematic of external valve unit of the present invention.
Wherein, a is to add blood sample, and b is to be washed and dried up, and c is to add enzyme standard liquid, and d is to be washed and blown
Dry, e is to add substrate solution, and f is to add terminate liquid.
Fig. 4 is the overall structure figure for showing this micro-fluidic chip.
Fig. 5 is the experimental result picture for showing to detect with the hepatitis B antigen standard items that micro-fluidic chip of the present invention is carried out.
Embodiment
Embodiment 1
Referring to Fig. 1 to Fig. 3, apparatus structure of the invention is as follows：
Main body is cuboid.Wherein micro-fluidic module is three-decker, including a cover plate A, substrate B and egative film C, three layers
Between pass through glue envelope realize alignment bonding.
Channel design includes referring to Fig. 1, the module of micro-fluidic chip：Reaction tank 7, waste liquid pool 8, four reagent inlets enter
Sample hole 5 and fluid channel.
Wherein：Reagent inlet 1,2,3 and 4, connects enzyme standard liquid bottle, Washing liquid bottle, the AB of pump valve structure by flexible pipe respectively
Substrate solution bottle and terminate liquid bottle.Each fluid channel is designed with miniature buffering area, and the width of fluid channel is 0.4mm, 1', 3',
4', 5' and 6 fluid channels are located at the top of substrate, and 2' fluid channels are located at the lower section of substrate.Passage is provided with above waste liquid pool.
Cover plate and egative film use PS materials, and substrate material can be quartzy, glass or PDMS, PMMA or PC etc.
Macromolecule polymer material.The thickness of chip is 7mm.Microfluidic channel unit can be by existing shaping microchip process technology
Into.
Chip will be coated with advance, i.e., be coated with the shadow region of the egative film C shown in Fig. 2, and 37 DEG C incubate 2 hours.Afterwards
Carry out the alignment bonding of chip.
The volume of reaction tank is 150ul, it is ensured that reaction solution can fully react in reaction tank.The volume of waste liquid pool is much larger than
Reaction tank, capacity is more than 1200ul.
Embodiment 2
Design and make and declined fluidic chip for the pump valve of blood hepatitis B quick detection, in order to be coated with and transparent
Degree, cover plate and egative film use PS materials, do not have particular/special requirement to substrate material, then substrate material can be quartz, glass,
The macromolecule polymer materials such as PDMS, PMMA or PC.With laser marking machine processing channel structure, alignment check is finally carried out.
Egative film coating region is coated with, 37 DEG C incubate 2 hours, the alignment bonding of chip is carried out afterwards.With micro note
Emitter injects the 1 of 40ul to inlet:5000 hepatitis B antigen standard items, encapsulating chip injection port.Liquid enters reaction tank, hatching
10 minutes.By valve unit, the injection air of reagent inlet 2 enters to be about to hepatitis B antigen sample and promotes waste liquid pool, and reagent enters afterwards
Mouth 2 injects cleaning solutions, irrigation channel and reaction tank, then the injection of reagent inlet 2 air, and cleaning solution flows into waste liquid pool.Reagent
Entrance 1 injects enzyme standard liquid, and enzyme standard liquid is reacted into reaction tank.After reaction 5 minutes, the injection air of reagent inlet 2, by enzyme standard liquid
Promote waste liquid pool.And repeat procedure below 5 times, and by valve unit, the injection cleaning solution of reagent inlet 2, irrigation channel and anti-
Ying Chi, backward reagent inlet 2 injection air, cleaning solution flows into waste liquid pool.Reagent inlet 3 injects AB substrate solutions, into reaction
Pond carries out reaction solution；The final injection of reagent inlet 4 terminate liquid, carries out terminating reaction.Time needed for whole detection process is big
About 45 minutes, acquired results were as shown in Figure 5.
Claims (7)
1. a kind of hepatitis B controlled based on pump valve examines micro-fluidic chip soon, it is characterised in that：The micro-fluidic chip is by egative film, base
Piece and cover plate composition, cover plate and egative film use PS materials, and substrate material can be the high scores such as quartz, glass, PDMS, PMMA or PC
Sub- polymeric material.
2. the hepatitis B controlled based on pump valve according to right 1 examines micro-fluidic chip soon, it is characterised in that：The micro-fluidic core
Piece is provided with reaction tank, waste liquid pool, fluid channel, sample holes, steam vent and the reagent inlet for connecting valve unit.Four reagents
Entrance and sample holes are connected by fluid channel with reaction tank, and reaction tank fluid channel is connected with waste liquid pool.Wherein connection cleaning solution examination
The fluid channel of agent bottle is located at the lower section of substrate.The fluid channel of ligase standard liquid reagent bottle, substrate solution bottle and terminate liquid bottle is located at
The top of substrate.
3. the hepatitis B controlled based on pump valve according to right 1 examines micro-fluidic chip soon, it is characterised in that：The micro-fluidic core
Piece needs connection outside valve unit that reagent is pumped in micro-fluidic chip.Four reagent inlets are respectively connecting to enzyme marking reagent
Bottle, cleaning solution reagent bottle, AB substrate solutions reagent bottle and terminate liquid reagent bottle.
4. the hepatitis B controlled based on pump valve according to right 1 examines micro-fluidic chip soon, it is characterised in that：The micro-fluidic core
The reaction tank of piece is connected by fluid channel with a sample holes, and sample introduction, after sample introduction is finished, sample holes are carried out using liquid-transfering gun
Sealed with outer seal device.
5. the hepatitis B controlled based on pump valve according to right 1 examines micro-fluidic chip soon, it is characterised in that：The micro-fluidic core
The waste liquid pool of piece has steam vent.
6. the hepatitis B controlled based on pump valve according to right 1 examines micro-fluidic chip soon, it is characterised in that：The micro-fluidic core
The egative film of piece is provided with coating region.After coating terminates, you can to three layers of progress calibration bonding of micro-fluidic chip.
7. the micro-fluidic chip according to right 1, the method for implementing hepatitis B detection is as follows：
1) by sample holes, antibody coating buffer is injected, antibody is coated with, 37 DEG C of incubations；
2) start external valve unit, cleaning solution is entered reagent inlet 2, rinse reaction tank, being again started up valve unit makes
Air enters reagent inlet 2, the cleaning solution in reaction tank is entered waste liquid pool, and dried up to reaction tank.
3) by sample holes, serum is injected, serum enters after reaction tank, sample holes are sealed, fully hatching 10 minutes；
4) start external valve unit, admit air into reagent inlet 2, the serum in reaction tank is flowed into waste liquid pool；
5) start external valve unit, cleaning solution is entered reagent inlet 2, rinse reaction tank, being again started up valve unit makes
Air enters reagent inlet 2, the cleaning solution in reaction tank is entered waste liquid pool, and dried up to reaction tank；
6) start external valve unit, enzyme standard liquid is entered reagent inlet 1, enzyme standard liquid enters after reaction tank, carry out antigen and resist
Precursor reactant；
7) start external valve unit, admit air into reagent inlet 2, the enzyme standard liquid in reaction tank is flowed into waste liquid pool.37
℃
8) incubate；
9) start external valve unit, cleaning solution is entered reagent inlet 2, rinse reaction tank, being again started up valve unit makes
Air enters reagent inlet 2, the cleaning solution in reaction tank is entered waste liquid pool, and dried up to reaction tank；Repeat this step 5
It is secondary；
10) by external valve unit, AB substrate solutions are made to enter reagent inlet 3, and enter reaction tank progress chromogenic reaction；
11) by external valve unit, terminate liquid is made to enter reagent inlet 4, and enter reaction tank progress terminating reaction, naked eyes
It can be seen that Preliminary detection result；
12) absorbance detection is carried out to the reagent in reaction tank with ELIASA, draws final detection result.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710206116.4A CN106984368A (en) | 2017-03-30 | 2017-03-30 | A kind of hepatitis B controlled based on pump valve examines micro-fluidic chip and analysis method soon |
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| CN201710206116.4A CN106984368A (en) | 2017-03-30 | 2017-03-30 | A kind of hepatitis B controlled based on pump valve examines micro-fluidic chip and analysis method soon |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108152351A (en) * | 2017-12-22 | 2018-06-12 | 大连大学 | A kind of micro cell for detecting formaldehyde and preparation method thereof |
| CN108514897A (en) * | 2018-04-11 | 2018-09-11 | 刘涵青 | A kind of micro-fluidic chip for middle school chemistry student experimenting |
| CN109590036A (en) * | 2018-12-17 | 2019-04-09 | 苏州汶颢微流控技术股份有限公司 | A kind of ELISA chip and its application method |
| CN111289762A (en) * | 2020-04-02 | 2020-06-16 | 厦门大学附属翔安医院 | Micro-fluidic chip sample adding device and testing method |
| WO2020244517A1 (en) * | 2019-06-03 | 2020-12-10 | 利多(香港)有限公司 | Microfluidic chip and manufacturing method therefor |
| CN114981011A (en) * | 2019-12-30 | 2022-08-30 | 伊鲁米那有限公司 | Flow cell assembly and associated reagent selector valve |
| CN115711964A (en) * | 2022-11-29 | 2023-02-24 | 北京泊菲莱科技有限公司 | Flow type photocatalytic rapid detection system and application method thereof |
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| CN105316224A (en) * | 2015-12-07 | 2016-02-10 | 中国科学院苏州生物医学工程技术研究所 | Full-automatic nucleic acid extraction and PCR amplification micro-fluidic chip and application method thereof |
| CN106053859A (en) * | 2016-08-02 | 2016-10-26 | 杭州霆科生物科技有限公司 | Centrifugal type glycosylated hemoglobin detection micro-fluidic chip |
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| US5744095A (en) * | 1995-11-14 | 1998-04-28 | Smith; Henry J. | Medical assay cassette |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108152351A (en) * | 2017-12-22 | 2018-06-12 | 大连大学 | A kind of micro cell for detecting formaldehyde and preparation method thereof |
| CN108514897A (en) * | 2018-04-11 | 2018-09-11 | 刘涵青 | A kind of micro-fluidic chip for middle school chemistry student experimenting |
| CN109590036A (en) * | 2018-12-17 | 2019-04-09 | 苏州汶颢微流控技术股份有限公司 | A kind of ELISA chip and its application method |
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| WO2020244517A1 (en) * | 2019-06-03 | 2020-12-10 | 利多(香港)有限公司 | Microfluidic chip and manufacturing method therefor |
| CN114981011A (en) * | 2019-12-30 | 2022-08-30 | 伊鲁米那有限公司 | Flow cell assembly and associated reagent selector valve |
| CN114981011B (en) * | 2019-12-30 | 2024-03-29 | 伊鲁米那有限公司 | Flow cell assembly and associated reagent selector valve |
| CN111289762A (en) * | 2020-04-02 | 2020-06-16 | 厦门大学附属翔安医院 | Micro-fluidic chip sample adding device and testing method |
| CN115711964A (en) * | 2022-11-29 | 2023-02-24 | 北京泊菲莱科技有限公司 | Flow type photocatalytic rapid detection system and application method thereof |
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Application publication date: 20170728 |