CN106983915A - Silk microfibre gelatin polyethylene glycol medical catheter and preparation method thereof - Google Patents
Silk microfibre gelatin polyethylene glycol medical catheter and preparation method thereof Download PDFInfo
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- CN106983915A CN106983915A CN201710373332.8A CN201710373332A CN106983915A CN 106983915 A CN106983915 A CN 106983915A CN 201710373332 A CN201710373332 A CN 201710373332A CN 106983915 A CN106983915 A CN 106983915A
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- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/04—Macromolecular materials
- A61L29/06—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
- A61L27/3604—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix characterised by the human or animal origin of the biological material, e.g. hair, fascia, fish scales, silk, shellac, pericardium, pleura, renal tissue, amniotic membrane, parenchymal tissue, fetal tissue, muscle tissue, fat tissue, enamel
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/58—Materials at least partially resorbable by the body
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- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/005—Ingredients of undetermined constitution or reaction products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
- A61L29/04—Macromolecular materials
- A61L29/044—Proteins; Polypeptides; Degradation products thereof
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- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
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- A61L29/00—Materials for catheters, medical tubing, cannulae, or endoscopes or for coating catheters
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- A61L29/148—Materials at least partially resorbable by the body
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Abstract
The present invention provides a kind of silk microfibre gelatin polyethylene glycol medical catheter, and prepared by the medical catheter per 100ml contains following component in solution：1 ~ 10g of silk microfibre, 10 ~ 100ml of the aqueous solution, 10% 2 ~ 30ml of gelatin solution, 1 ~ 10g of polyethylene glycol 2000.The present invention prepares silk microfibre gelatin polyethylene glycol catheter holder material using physical mixing processes, and material price is cheap, and preparation method is simply easily operated, it is to avoid the cellulotoxic side effect produced using crosslinking agent, good biocompatibility.Suitable for blood vessel, peripheral nerve conduit application, it is easy to metabolism in vivo.
Description
Technical field
The invention belongs to medical material tech field, and in particular to a kind of silk microfibre-gelatin-polyethylene glycol is medical is led
Pipe and preparation method thereof.
Background technology
Medical catheter is the necessity of medical act already, it usually needs implantation human body carries out traumatic diagnosis and treatment, for example, exist
Intravascular inlying catheter, central vein catheter, in the urinary tract treatment in use catheter, trachea cannula, be all in general curative
Operation necessity and the medical act frequently carried out.Clinically due to caused by wound peripheral nerve defection it is relatively conventional, wherein long
The neurologic defect of distance cannot rely on end-to-end suture to make up during neurological deficits, then need to bridge reparation by conduit.
In addition to above-mentioned conventional medical conduit, as medical technology is fast-developing, the conduit of many tube chamber and composite pipeline forms is also produced successively
It is raw, and put and stay in internal time relative growth, also turned into carry out now blood flow monitoring, transfusion, haemodialysis, administration and
The important tool of the medical acts such as intravenous nutrition support, it is shown that the utilization of medical catheter and demand acutely increase.
The effect harmless to the human body of silk microfibre, it is safe and reliable, with good biocompatibility, suitable for succeeding in developing
Energy property material, it can be applied to the various fields such as medicine, health, food, chemical industry.
Gelatin is one of very important native biopolymer material, is the partial hydrolysate of collagen, cheap and easy to get,
With good biocompatibility and degradability, food, medicine and chemical industry have been widely used in it.
Polyethylene glycol is the extremely wide polyether high molecular compound of medical application, can be applied to medicine, health, food,
The various fields such as chemical industry, with hydrophily, non-toxic and immunology inertia, internal injection macromolecule is turned into by FDA approvals.Profit
With the amphipathic copolymer containing polyethylene glycol is in medical macromolecular materials adsorption, retention and is grafted, it can improve and be connect with blood
The biocompatibility of tactile medical macromolecular materials.
Although silk microfibre, gelatin and polyethylene glycol possess above-mentioned each advantage, at present by silk microfibre, gelatin and
The mixture or polymer of polyethylene glycol are rarely reported for preparing medical catheter.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of silk microfibre-gelatin-polyethylene glycol medical catheter and its
Preparation method, material price is cheap, and preparation method is simply easily operated, it is to avoid the cellulotoxic side effect produced using crosslinking agent,
Good biocompatibility.Suitable for blood vessel, peripheral nerve conduit application, it is easy to metabolism in vivo.
In order to solve the above technical problems, a kind of silk microfibre-gelatin-polyethylene glycol of embodiments of the invention offer is medical
Conduit, prepared by the medical catheter per 100ml contains following component in solution：1 ~ 10g of silk microfibre, 10 ~ 100ml of the aqueous solution,
10% 2 ~ 30ml of gelatin solution, 1 ~ 10g of polyethylene glycol 2000.
Wherein, prepared by the medical catheter per 100ml contains following component in solution：3 ~ 7g of silk microfibre, the aqueous solution 20 ~
80ml, 10% 10 ~ 25ml of gelatin solution, 3 ~ 8g of polyethylene glycol 2000.
Preferably, prepared by the medical catheter per 100ml contains following component in solution：Silk microfibre 6g, the aqueous solution
60ml, 10% gelatin solution 20ml, polyethylene glycol 2000 5g.
Wherein, the preparation method of the silk microfibre includes following three stage：
A, from cocoon prepare degumming silk fiber；
B, degumed silk fiber is hydrolyzed into micron order silk fiber；
C, silk fiber wash, neutralize and be lyophilized into silk fiber powder.
Further, stage b, degumed silk fiber is hydrolyzed into concretely comprising the following steps for micron order silk fiber：By sodium hydroxide plus
Enter into distilled water, when 30% ~ 40% NaOH exothermic reactions dissolving, add dry degumming silk fiber and stirred with scraper,
It is hydrolyzed, the weight ratio of the sodium hydroxide, distilled water and degumming silk fiber is 1：2.5~3.5：0.15~0.25；
Stage c, silk fiber wash, neutralize and be lyophilized into concretely comprising the following steps for silk fiber powder：
C-1, the water of 8 ~ 10 times of distilled water volumes is added in the reactant mixture of stage b, and with centrifugal force 2500 ~
3000xg is centrifuged 5 ~ 8 minutes；
C-2, supernatant is abandoned, in the water that silk fiber is resuspended in 8 ~ 10 times of distilled water volumes, stir and centrifuge；
C-3,5 ~ 8 step c-2 of repetition, remove excess base；
C-4, pH value determination, and use salt acid for adjusting pH to 7.0；
C-5, the silk fiber solution of neutralization is centrifuged with 3000 ~ 4000 rpm again 5 ~ 20min and be resuspended in water, repeat 3 ~ 5
It is secondary, finally fiber suspension in PBS and is freezed, silk fiber powder is obtained.
Preferably, stage b, degumed silk fiber is hydrolyzed into concretely comprising the following steps for micron order silk fiber：By 1.75g hydrogen-oxygen
Change sodium to be added in 5mL distilled water, when about 35% NaOH exothermic reactions dissolving, the degumed silk for adding 0.35g drying is fine
Tie up and stirred with scraper, is hydrolyzed.To obtain 400 ~ 500 μm and medium 150 ~ 200 μm of superfine fibres, the time of hydrolysis
30s and 180s is carried out respectively.In order to obtain 10 ~ 20 μm of silk fiber, reactant mixture is placed in 60s in boiling water bath fast to help
Speed hydrolysis；
Stage c, silk fiber wash, neutralize and be lyophilized into concretely comprising the following steps for silk fiber powder：
C-1,45ml water is added in the reactant mixture of stage b, and centrifuged 5 minutes with centrifugal force 2800xg；
C-2, supernatant is abandoned, silk fiber is resuspended in 50mL water, stirs and centrifuges；
C-3,5 ~ 8 step c-2 of repetition, remove excess base；
C-4, pH value determination, and use salt acid for adjusting pH to 7.0；
C-5, the silk fiber solution of neutralization is centrifuged with 3500rpm again 5min and be resuspended in water, repeat 3 ~ 5 times, finally will
Fiber suspension is in PBS and freezes, and obtains silk fiber powder.
Wherein, 10% gelatin solution is formulated by gelatin and pure water, and the mass percent of the gelatin and pure water is
1:9。
The embodiment of the present invention also provides a kind of above-mentioned silk microfibre-gelatin-polyethylene glycol medical catheter, including as follows
Step：
（1）Prepare silk microfibre solution：The silk microfibre of formula ratio is taken to be dissolved in 10 ~ 100ml aqueous solution, heating stirring
It is completely dissolved it；
（2）10% gelatin solution of formula ratio is weighed, 10% gelatin solution is poured slowly into step（1）The silk microfibre of preparation is molten
In liquid, glass bar is sufficiently stirred for dissolving；
（3）The polyethylene glycol 2000 of formula ratio is weighed, step is added（2）In gained mixed liquor, 55 ~ 65 °C of ultrasonic agitations make it
Fully dissolving；
（4）Utilize 10ml syringe aspiration steps（3）Medical catheter prepare solution, be expelled in catheter mold, fix and contain
The early stage catheter mold of medical catheter preparation solution, lies in the big ware of glass, is placed in refrigerator freezing；
（5）Take out step（4）Early stage catheter mold in refrigerator, the outer layer of early stage of decorporating catheter mold forms parcel inner core
Later stage catheter mold, later stage catheter mold is sandwiched in lyophilized bottle with tweezers, covers bottle stopper, and level is put into refrigerator, freezing；
（6）Take out step（5）Lyophilized bottle after middle freezing, it is self cooling lyophilized after using freeze drier, lyophilized bottle is freezed completely
Lyophilized bottle is removed on dry machine, and takes out later stage catheter mold, the inner core for later stage catheter mold of carefully decorporating obtains silk fento
Dimension-gelatin-polyethylene glycol medical catheter；
（7）The silk prepared microfibre-gelatin-polyethylene glycol medical catheter is put into hermetic bag, marked title and
Preparation time, is preserved in shady and cool, dry director's phase, uses preceding taking-up medical catheter Co60Irradiation, sterilization, it is standby.
Preferably, the preparation method of above-mentioned silk microfibre-gelatin-polyethylene glycol medical catheter comprises the following steps：
(1) ' prepare silk microfibre solution：6g silk microfibres are taken to be dissolved in the 60ml aqueous solution, heating stirring makes its complete
Dissolving；
(2) ' weighing 20ml 10% gelatin solution, step is poured slowly into by 10% gelatin solution（1）The silk microfibre of preparation is molten
In liquid, glass bar is sufficiently stirred for dissolving；
(3) ' and 5g polyethylene glycol 2000s are weighed, add step（2）In gained mixed liquor, 65 °C of ultrasonic agitation 2.5h make its abundant
Dissolving；
(4) ' using 10ml syringes draw 8ml steps (3) ' medical catheter prepare solution, be expelled in medical catheter mould,
The early stage catheter mold of the preparation solution containing medical catheter is fixed, lies in the big ware of glass, is placed in refrigerator, -20 DEG C freezed
Night；
(5) the early stage catheter mold in ' taking-up step (4) ' refrigerator, the outer layer of early stage of decorporating catheter mold forms parcel inner core
Later stage catheter mold, later stage catheter mold sandwiches later stage catheter mold in lyophilized bottle with tweezers, bottle stopper is covered, level is put
Enter refrigerator, -20 DEG C of freezing 2h；
(6) ' open freeze drier, connect freeze drier and vavuum pump, dry built-in temperature to be frozen be down to -40 DEG C with
Under, vacuum is when being down to 0.02Pa, by step (5) ' in freeze bottle and move on in freeze drier, freeze 12h, bottle to be freezed freezes completely
After dry, remove lyophilized bottle and take out later stage catheter mold, the inner core for later stage catheter mold of carefully decorporating, obtain silk microfibre-
Gelatin-polyethylene glycol medical catheter；
(7) ' silk prepared microfibre-gelatin-polyethylene glycol medical catheter is put into hermetic bag, marked title and
Preparation time, is preserved in shady and cool, dry director's phase, uses preceding taking-up medical catheter Co60Irradiation, sterilization, it is standby.
Wherein, the medical catheter mould includes outer tube, inner core, lower closure and seaming chuck, and the lower closure is inverted T-shaped knot
Structure, it is interior provided with upper end opening, the lower closure centre bore of lower end closed, and the lower end of the inner core is inserted in lower closure centre bore,
The upper end of the inner core penetrates from the lower end of outer tube and extend out to outer tube upper end, and the top of the lower closure inserts in outer tube pipe
Intracavitary, lower limit is in the lower section of outer tube；
The seaming chuck is T-shaped structure, the seaming chuck centre bore provided with upper and lower side opening in it, and the seaming chuck is set in outer
Between pipe and inner core, and its underpart is inserted in outer tube tube chamber, top is limited in above outer tube, between the seaming chuck and lower closure
Gap prepare solution fill area for medical catheter.
The above-mentioned technical proposal of the present invention has the beneficial effect that：The present invention prepares silk fento using physical mixing processes
Dimension-gelatin-polyethylene glycol catheter holder material, material price is cheap, and preparation method is simply easily operated, it is to avoid use crosslinking agent
The cellulotoxic side effect of generation, good biocompatibility.Suitable for blood vessel, peripheral nerve conduit application, it is easy to metabolism in vivo.
Brief description of the drawings
The vertical section scanning electron microscope (SEM) photograph for the silk microfibre-gelatin-polyethylene glycol medical catheter that Fig. 1 is prepared for the present invention；
The structural representation for the silk microfibre-gelatin-polyethylene glycol medical catheter that Fig. 2 is prepared for the present invention；
The structure decomposition figure for the medical catheter mould that Fig. 3 uses for the present invention.
Description of reference numerals：
1st, outer tube；2nd, inner core；3rd, lower closure；4th, seaming chuck.
Embodiment
To make the technical problem to be solved in the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing and tool
Body embodiment is described in detail.
The present invention provides a kind of scanning electron microscope (SEM) photograph silk microfibre as shown in Figure 1 and Figure 2-gelatin-polyethylene glycol is medical and led
Pipe, wherein Fig. 1（a）For silk microfibre-gelatin-polyethylene glycol medical catheter vertical section scanning electron microscope (SEM) photograph, Fig. 1（b）For Fig. 1
（a）Partial enlarged drawing at middle square frame.Prepared by the medical catheter per 100ml contains following component in solution：Silk microfibre 1 ~
10g, 10 ~ 100ml of the aqueous solution, 10% 2 ~ 30ml of gelatin solution, 1 ~ 10g of polyethylene glycol 2000.
The preparation method of above-mentioned silk microfibre-gelatin-polyethylene glycol medical catheter comprises the following steps：
（1）Prepare silk microfibre solution：The silk microfibre of formula ratio is taken to be dissolved in 10 ~ 100ml aqueous solution, heating stirring
It is completely dissolved it；
（2）10% gelatin solution of formula ratio is weighed, 10% gelatin solution is poured slowly into step（1）The silk microfibre of preparation is molten
In liquid, glass bar is sufficiently stirred for dissolving；
（3）The polyethylene glycol 2000 of formula ratio is weighed, step is added（2）In gained mixed liquor, 55 ~ 65 °C of ultrasonic agitations make it
Fully dissolving；
（4）Utilize 10ml syringe aspiration steps（3）Medical catheter prepare solution, be expelled in catheter mold, fix and contain
The early stage catheter mold of medical catheter preparation solution, lies in the big ware of glass, is placed in refrigerator freezing；
（5）Take out step（4）Early stage catheter mold in refrigerator, the outer layer of early stage of decorporating catheter mold forms parcel inner core
Later stage catheter mold, later stage catheter mold is sandwiched in lyophilized bottle with tweezers, covers bottle stopper, and level is put into refrigerator, freezing；
（6）Take out step（5）Lyophilized bottle after middle freezing, it is self cooling lyophilized after using freeze drier, lyophilized bottle is freezed completely
Lyophilized bottle is removed on dry machine, and takes out later stage catheter mold, the inner core for later stage catheter mold of carefully decorporating obtains silk fento
Dimension-gelatin-polyethylene glycol medical catheter；
（7）The silk prepared microfibre-gelatin-polyethylene glycol medical catheter is put into hermetic bag, marked title and
Preparation time, is preserved in shady and cool, dry director's phase, uses preceding taking-up medical catheter Co60Irradiation, sterilization, it is standby.
Prepare silk microfibre-gelatin-medical catheter mould structure such as Fig. 3 institutes used in polyethylene glycol medical catheter
Show, including outer tube 1, inner core 2, lower closure 3 and seaming chuck 4, the lower closure 3 is inverted T-shaped structure, in it provided with upper end it is open,
The lower closure centre bore of lower end closed, the lower end of the inner core 2 is inserted in lower closure centre bore, and the upper end of the inner core 2 is outside
The lower end of pipe 1 is penetrated and extend out to the upper end of outer tube 1, and the top of the lower closure 3 is inserted in the tube chamber of outer tube 1, lower limit
In the lower section of outer tube 1.
The seaming chuck 4 is T-shaped structure, the seaming chuck centre bore provided with upper and lower side opening in it, 4 sets of the seaming chuck
Loaded between outer tube 1 and inner core 2, and its underpart is inserted in the tube chamber of outer tube 1, top is limited in the top of outer tube 1, the seaming chuck 4
Gap between lower closure 3 prepares solution fill area for medical catheter.
When preparing silk microfibre-gelatin-polyethylene glycol medical catheter using above-mentioned medical catheter mould, first by inner core
Lower end inserting to it is lower closure centre bore in, then the upper end of inner core is penetrated and extend out on outer tube upper end from the lower end of outer tube
Side, is inserted in outer tube tube chamber, lower limit is in the lower section of outer tube to the top of lower closure.From the upper port of outer tube to outer tube tube chamber
Interior injection medical catheter prepares solution, then by seaming chuck through on inner core and being pressed downward medical catheter and prepare solution, to pushing
The bottom of head is inserted in outer tube tube chamber, top is limited in above outer tube, so far, the gap shaping silkworm between seaming chuck and lower closure
Silk microfibre-gelatin-polyethylene glycol medical catheter.
Following 3 parts of samples are specifically prepared according to above-mentioned preparation method, and the porosity and mechanical strength of 3 parts of samples are entered
Row compares, and the component value and each experiment parameter of 3 parts of samples are the more excellent combinations under current experiment condition, but be not limit it is of the invention
The unique combinations of protection domain.
Sample G1 preparation：
(1) ' prepare silk microfibre solution：6g silk microfibres are taken to be dissolved in the 60ml aqueous solution, heating stirring makes its complete
Dissolving；
(2) ' weighing 20ml 10% gelatin solution, step is poured slowly into by 10% gelatin solution（1）The silk microfibre of preparation is molten
In liquid, glass bar is sufficiently stirred for dissolving；
(3) ' and 5g polyethylene glycol 2000s are weighed, add step（2）In gained mixed liquor, 65 °C of ultrasonic agitation 2.5h make its abundant
Dissolving；
(4) ' using 10ml syringes draw 8ml steps (3) ' medical catheter prepare solution, be expelled in medical catheter mould,
The early stage catheter mold of the preparation solution containing medical catheter is fixed, lies in the big ware of glass, is placed in refrigerator, -20 DEG C freezed
Night；
(5) the early stage catheter mold in ' taking-up step (4) ' refrigerator, the outer layer of early stage of decorporating catheter mold forms parcel inner core
Later stage catheter mold, later stage catheter mold sandwiches later stage catheter mold in lyophilized bottle with tweezers, bottle stopper is covered, level is put
Enter refrigerator, -20 DEG C of freezing 2h；
(6) ' open freeze drier, connect freeze drier and vavuum pump, dry built-in temperature to be frozen be down to -40 DEG C with
Under, vacuum is when being down to 0.02Pa, by step (5) ' in freeze bottle and move on in freeze drier, freeze 12h, bottle to be freezed freezes completely
After dry, remove lyophilized bottle and take out later stage catheter mold, the inner core for later stage catheter mold of carefully decorporating, obtain silk microfibre-
Gelatin-polyethylene glycol medical catheter；
(7) ' silk prepared microfibre-gelatin-polyethylene glycol medical catheter is put into hermetic bag, marked title and
Preparation time, is preserved in shady and cool, dry director's phase, uses preceding taking-up medical catheter Co60Irradiation, sterilization, it is standby.
Sample G2 is with G3 in addition to constituent part content is different, and remaining implementation process is with sample G1, sample G2 and G3 prescription
Content such as following table one.
Table one：
| Formula | G2 | G3 |
| Silk microfibre | 2 g | 4g |
| The aqueous solution | 80mL | 40mL |
| 10% gelatin solution | 20mL | 8mL |
| Polyethylene glycol 2000 | 1g | 3g |
Porosity is carried out to sample G1, G2, G3 respectively and pulling force loading is detected, as a result such as table two.
Table two：
| Sample | Porosity | Maximum loading |
| G1 | > 45% | 4.102N |
| G2 | Shaping is bad | 1.892 N |
| G3 | <40% | 3.981N |
As shown in Table 2, the medical catheter that prepared by sample G1 prescriptions has good porosity and mechanical strength, preferably
Cell culture and the requirement of cellular products research field are met, is the good medical catheter for studying organizational project.
The above-mentioned technical proposal of the present invention has the beneficial effect that：
（1）Formula material wide material sources of the present invention, it is cheap, with medical macromolecular materials adsorption, retention
And grafting functional.
（2）Medical catheter nontoxicity and immunology inertia prepared by formula of the present invention, improve and contacting blood
Medical macromolecular materials biocompatibility.
（3）The medical catheter that formula of the present invention is prepared by mould has abundant three-dimensional structure, can be nerve
The conveying of nutriment needed for cell growth process provide necessary approach, for nerve cell growth provide it is necessary
Inducing action and necessary growing space.
（4）The medical catheter that preparation method of the present invention is obtained has good mechanical strength, and maximum loading is
4.102N。
Described above is the preferred embodiment of the present invention, it is noted that for those skilled in the art
For, on the premise of principle of the present invention is not departed from, some improvements and modifications can also be made, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (9)
1. a kind of silk microfibre-gelatin-polyethylene glycol medical catheter, it is characterised in that the medical catheter per 100ml prepares molten
Contain following component in liquid：1 ~ 10g of silk microfibre, 10 ~ 100ml of the aqueous solution, 10% 2 ~ 30ml of gelatin solution, polyethylene glycol
2000 1~10g。
2. silk microfibre-gelatin-polyethylene glycol medical catheter according to claim 1, it is characterised in that per 100ml
Medical catheter prepare in solution contain following component：3 ~ 7g of silk microfibre, 20 ~ 80ml of the aqueous solution, 10% gelatin solution 10 ~
25ml, 3 ~ 8g of polyethylene glycol 2000.
3. silk microfibre-gelatin-polyethylene glycol medical catheter according to claim 1, it is characterised in that per 100ml
Medical catheter prepare in solution contain following component：Silk microfibre 6g, aqueous solution 60ml, 10% gelatin solution 20ml, poly- second
The 5g of glycol 2000.
4. the silk microfibre-gelatin-polyethylene glycol medical catheter according to any one of claim 1 ~ 3, its feature exists
In the preparation method of the silk microfibre includes following three stage：
A, from cocoon prepare degumming silk fiber；
B, degumed silk fiber is hydrolyzed into micron order silk fiber；
C, silk fiber wash, neutralize and be lyophilized into silk fiber powder.
5. silk microfibre-gelatin-polyethylene glycol medical catheter according to claim 4, it is characterised in that stage b, general
Degumed silk fiber is hydrolyzed into concretely comprising the following steps for micron order silk fiber：Sodium hydroxide is added in distilled water, when 30% ~ 40%
When NaOH exothermic reactions are dissolved, add dry degumming silk fiber and stirred with scraper, is hydrolyzed, the sodium hydroxide, steaming
Distilled water and the weight of degumming silk fiber ratio are 1：2.5~3.5：0.15~0.25；
Stage c, silk fiber wash, neutralize and be lyophilized into concretely comprising the following steps for silk fiber powder：
C-1, the water of 8 ~ 10 times of distilled water volumes is added in the reactant mixture of stage b, and with centrifugal force 2500 ~
3000xg is centrifuged 5 ~ 8 minutes；
C-2, supernatant is abandoned, in the water that silk fiber is resuspended in 8 ~ 10 times of distilled water volumes, stir and centrifuge；
C-3,5 ~ 8 step c-2 of repetition, remove excess base；
C-4, pH value determination, and use salt acid for adjusting pH to 7.0；
C-5, the silk fiber solution of neutralization is centrifuged with 3000 ~ 4000 rpm again 5 ~ 20min and be resuspended in water, repeat 3 ~ 5
It is secondary, finally fiber suspension in PBS and is freezed, silk fiber powder is obtained.
6. the silk microfibre-gelatin-polyethylene glycol medical catheter according to any one of claim 1 ~ 3, its feature exists
In 10% gelatin solution is formulated by gelatin and pure water, and the mass percent of the gelatin and pure water is 1:9.
7. a kind of preparation side of silk microfibre-gelatin-polyethylene glycol medical catheter as any one of claim 1 ~ 6
Method, it is characterised in that comprise the following steps：
（1）Prepare silk microfibre solution：The silk microfibre of formula ratio is taken to be dissolved in 10 ~ 100ml aqueous solution, heating stirring
It is completely dissolved it；
（2）10% gelatin solution of formula ratio is weighed, 10% gelatin solution is poured slowly into step（1）The silk microfibre of preparation is molten
In liquid, glass bar is sufficiently stirred for dissolving；
（3）The polyethylene glycol 2000 of formula ratio is weighed, step is added（2）In gained mixed liquor, 55 ~ 65 °C of ultrasonic agitations make it
Fully dissolving；
（4）Utilize 10ml syringe aspiration steps（3）Medical catheter prepare solution, be expelled in catheter mold, fix and contain
The early stage catheter mold of medical catheter preparation solution, lies in the big ware of glass, is placed in refrigerator freezing；
（5）Take out step（4）Early stage catheter mold in refrigerator, the outer layer of early stage of decorporating catheter mold forms parcel inner core
Later stage catheter mold, later stage catheter mold is sandwiched in lyophilized bottle with tweezers, covers bottle stopper, and level is put into refrigerator, freezing；
（6）Take out step（5）Lyophilized bottle after middle freezing, it is self cooling lyophilized after using freeze drier, lyophilized bottle is freezed completely
Lyophilized bottle is removed on dry machine, and takes out later stage catheter mold, the inner core for later stage catheter mold of carefully decorporating obtains silk fento
Dimension-gelatin-polyethylene glycol medical catheter；
（7）The silk prepared microfibre-gelatin-polyethylene glycol medical catheter is put into hermetic bag, marked title and
Preparation time, is preserved in shady and cool, dry director's phase, uses preceding taking-up medical catheter Co60Irradiation, sterilization, it is standby.
8. the preparation method of silk microfibre-gelatin-polyethylene glycol medical catheter according to claim 7, its feature exists
In comprising the following steps：
(1) ' prepare silk microfibre solution：6g silk microfibres are taken to be dissolved in the 60ml aqueous solution, heating stirring makes its complete
Dissolving；
(2) ' weighing 20ml 10% gelatin solution, step is poured slowly into by 10% gelatin solution（1）The silk microfibre of preparation is molten
In liquid, glass bar is sufficiently stirred for dissolving；
(3) ' and 5g polyethylene glycol 2000s are weighed, add step（2）In gained mixed liquor, 65 °C of ultrasonic agitation 2.5h make its abundant
Dissolving；
(4) ' using 10ml syringes draw 8ml steps (3) ' medical catheter prepare solution, be expelled in medical catheter mould,
The early stage catheter mold of the preparation solution containing medical catheter is fixed, lies in the big ware of glass, is placed in refrigerator, -20 DEG C freezed
Night；
(5) the early stage catheter mold in ' taking-up step (4) ' refrigerator, the outer layer of early stage of decorporating catheter mold forms parcel inner core
Later stage catheter mold, later stage catheter mold sandwiches later stage catheter mold in lyophilized bottle with tweezers, bottle stopper is covered, level is put
Enter refrigerator, -20 DEG C of freezing 2h；
(6) ' open freeze drier, connect freeze drier and vavuum pump, dry built-in temperature to be frozen be down to -40 DEG C with
Under, vacuum is when being down to 0.02Pa, by step (5) ' in freeze bottle and move on in freeze drier, freeze 12h, bottle to be freezed freezes completely
After dry, remove lyophilized bottle and take out later stage catheter mold, the inner core for later stage catheter mold of carefully decorporating, obtain silk microfibre-
Gelatin-polyethylene glycol medical catheter；
(7) ' silk prepared microfibre-gelatin-polyethylene glycol medical catheter is put into hermetic bag, marked title and
Preparation time, is preserved in shady and cool, dry director's phase, uses preceding taking-up medical catheter Co60Irradiation, sterilization, it is standby.
9. the preparation method of the silk microfibre-gelatin-polyethylene glycol medical catheter according to claim 7 or 8, its feature
It is, the medical catheter mould includes outer tube, inner core, lower closure and seaming chuck, and the lower closure is inverted T-shaped structure, in it
Lower closure centre bore provided with upper end opening, lower end closed, the lower end of the inner core is inserted in lower closure centre bore, the inner core
Upper end penetrated from the lower end of outer tube and extend out to outer tube upper end, the top of the lower closure is inserted in outer tube tube chamber, under
Portion is limited in the lower section of outer tube；The seaming chuck is T-shaped structure, the seaming chuck centre bore provided with upper and lower side opening in it, institute
State seaming chuck to be set between outer tube and inner core, and its underpart is inserted in outer tube tube chamber, top is limited in above outer tube, it is described on
Gap between pressure head and lower closure prepares solution fill area for medical catheter.
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| CN201710373332.8A CN106983915B (en) | 2017-05-24 | 2017-05-24 | Silk microfibre-gelatin-polyethylene glycol medical catheter and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628327A (en) * | 2019-10-11 | 2019-12-31 | 南通市乐佳涂料有限公司 | Environment-friendly waterproof building coating and preparation method thereof |
| CN110655810A (en) * | 2019-10-11 | 2020-01-07 | 南通市乐佳涂料有限公司 | Environment-friendly high-efficiency building coating and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036802A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and the preparation method |
| CN101579246A (en) * | 2009-05-31 | 2009-11-18 | 苏州大学 | Artificial silk fibroin nano-fiber nerve repair conduit and preparation method thereof |
| WO2011019211A2 (en) * | 2009-08-12 | 2011-02-17 | 서울대학교 산학협력단 | Silk nanofiber nerve conduit and method for producing thereof |
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2017
- 2017-05-24 CN CN201710373332.8A patent/CN106983915B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101036802A (en) * | 2006-12-30 | 2007-09-19 | 苏州大学 | Antheraea pernyi silk fibrion biology medicine material and the preparation method |
| CN101579246A (en) * | 2009-05-31 | 2009-11-18 | 苏州大学 | Artificial silk fibroin nano-fiber nerve repair conduit and preparation method thereof |
| WO2011019211A2 (en) * | 2009-08-12 | 2011-02-17 | 서울대학교 산학협력단 | Silk nanofiber nerve conduit and method for producing thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110628327A (en) * | 2019-10-11 | 2019-12-31 | 南通市乐佳涂料有限公司 | Environment-friendly waterproof building coating and preparation method thereof |
| CN110655810A (en) * | 2019-10-11 | 2020-01-07 | 南通市乐佳涂料有限公司 | Environment-friendly high-efficiency building coating and preparation method thereof |
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| CN106983915B (en) | 2019-09-06 |
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