CN107287155A - A kind of method for improving fat cell transplanting success - Google Patents
A kind of method for improving fat cell transplanting success Download PDFInfo
- Publication number
- CN107287155A CN107287155A CN201710402513.9A CN201710402513A CN107287155A CN 107287155 A CN107287155 A CN 107287155A CN 201710402513 A CN201710402513 A CN 201710402513A CN 107287155 A CN107287155 A CN 107287155A
- Authority
- CN
- China
- Prior art keywords
- cell
- fat
- tissue
- suspension
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Rheumatology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of method for improving fat cell transplanting success, it is related to cell transplant techniques field.The method of described raising fat cell transplanting success is：Step 1: fat stem cell is obtained, Step 2: fat cell is obtained, transplanting, the fat stem cell of harvest culture, is counted while fat cell is handled；Fat stem cell and fat cell are pressed 2:1 ratio is mixed；Obtained cell suspension carries out being transplanted to corresponding site.The obtaining step of fat stem cell is relatively simple, fat stem cell can secrete a variety of growing nutrient factors, improve the microenvironment inside histoorgan, therefore, the death caused after fat cell filling due to a lack of nutrient can be undoubtedly substantially improved using autologous fat stem cell and fat cell co-transplantation, so as to improve the transplanting success of fat cell.
Description
Technical field
The present invention relates to a kind of method for improving fat cell transplanting success, belong to cell transplant techniques field.
Background technology
With advancing age, skin can engender the aging phenomena, shaping such as relaxation, elasticity decrease and local dent
Surgery is often extracted out and the position that patient needs to fill is added to after patient body superabundant fats, purifying fat cell, not only quick beautiful
Change body curve, and be preferably minimized the damage of fat.It is general to use after low-speed centrifugal, purification, purified treatment, select completely
Fat cell particle, using fine amalgamation injection technique, multi-level multiple spot carries out temple, apple flesh, cheek, forehead, nose
Son, mouth week etc. face filling/smoothing wrinkle/moulding etc..
The upper century-old history of autologous fat transplantation, due to its abundance, draw materials it is easy, simple to operate, full outer
Shape is good, local reaction is small, the advantages of no rejection, and has preferable feel after transplanting and be easily accepted by patients, and can obtain
Preferably moulding and other effects the attention for enjoying cosmetic plastic surgery doctor.Therefore, in recent years, autologous fat transplantation beauty has been
One of beauty mode received the most as beauty lovers.
Current autologous fat is that plastic surgery carries out Soft-tissue operation and moulding conventional packing material, autologous fat filling
Clinically it is widely used, is always orthopedic study hotspot after being reported first from Neuber in 1893.Over nearly 100 years,
Autologous fat filling application constantly extends, available for enlarging the bosom, grand jaw, augmentation rhinoplasty, applied at aging face, limbs it is not right
Claim, caused by depressed scar and a variety of causes at tissue defect etc..
Early stage autologous fat fills surgical effect existing defects, and the fat absorption rate of filling is high, causes autologous fat quantity
Reduce, the adverse reactions such as infection, liquefaction, necrosis, calcification and tumour formation occurs in filling position.After Coleman technologies are born
The survival rate of transplant fat is improved to a certain extent, and autologous fat filling is just gradually widely used in clinic, but autologous fat
Absorptivity it is still higher.The fat of acquisition is injected soft tissue injury position, i.e. fat cell transplanting skill by fatty liposuction technology
Art, but the problem of there is the low survival rate of transplant fat and high-absorbility that remain unchanged.
Although autologous fat cell transplantation enjoys the attention of cosmetic plastic surgery doctor, do not turn into a kind of yet at present
Conventional operation method, main cause generally accounts for the 40%-50% of initial fatty volume for fat of being survived after transplanting, and some are reported
Adipose tissue at most only survives 10%, and this causes the application of simple autologous fat cell infusion to be restricted, and adipose tissue is inhaled
Receive and finally substituted by fibrous connective tissue.The reason for fat cell transplanting success is low may have at 2 points, and one is ripe
Fat cell is low to the tolerance of damage, does not possess the ability that propagation updates；Two be transplant fat tissue neovascularization speed
Degree and degree are low, cause the adipose tissue of transplanting to lack the shortage that blood supply causes oxygen and nutrition, so that effect is not good.
Majority research thinks that local environment anoxic is the fatty most important factor survived of influence after fat cell filling.It is autologous
Initial stage after fat filling, fat is in anoxic and ischemic environment, and nutrient is obtained by the effect of receptor area infiltration of surrounding tissues；It is newborn
After capillary is formed, acceptor regions revascularization, fat can obtain nutrient by blood fortune.Fat cell is non-under anoxia condition
It is often sensitive, when partial pressure of oxygen is too low, during more than its tolerance threshold, Adipocyte Apoptosis.It can be seen that, the pass survived after autologous fat filling
Key is to rebuild blood fortune early stage.If adding fat-derived stem cells in implanting tissue, improve stem cell therein and fat is thin
Born of the same parents' ratio, can make the overall survival of implanting tissue increases, and this is that ADSCs assists autologous fat transplantation art（Cell-Assisted
Lipotransfer, CAL）.
Survival rate is relatively low during existing adipocyte culture, and complex steps, it is therefore desirable to which research one kind can be improved
The method of fat cell transplanting success.
The content of the invention
In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of raising fat cell transplanting success
Method.
The method of raising fat cell transplanting success of the present invention is：Step 1: fat stem cell obtain, 1-1, will inhale
The adipose tissue in fat source is put into precooling containing in 1% dual anti-physiological saline, seals, under cryogenic conditions, sends experiment at 2-8 DEG C back to
Room；1-2, the haemocyte and fragment of tissue for remaining above-mentioned adipose tissue normal saline flushing, removal, are put into adipose tissue
In centrifuge tube, initial volume is recorded, isometric digestive juice is added into tissue, described digestive juice is by 0.25% pancreatin, 0.1%
Type i collagen enzyme, with 1:1 ratio mixed preparing is formed, be put into 37 DEG C of gas bath oscillators and digest 30min, then will have been digested
Tissue stands 5min, and now liquid level is divided into 3 layers, and upper strata yellow oil is the fat drips of broken fat cell release, and middle level is
Bulky grain adipose cell layer, or mix a small amount of fiber, lower floor is mononuclearcell layer；1-3, upper strata and middle layer cells are discarded,
By the cell suspension of lower floor with 1500r/min, supernatant is abandoned after centrifuging 10min, obtains centrifuging the fat stem cell group of bottom of the tube, uses
Non-animal source serum L-DMEM nutrient solutions are resuspended, and density is adjusted to 1 × 105Individual/mL, being then seeded in culture area is
25cm2Tissue Culture Flask in；1-4, at 37 DEG C 5% CO2Under the conditions of cultivate；1-5, cover with blake bottle when fat stem cell
Two are passed by one to be passed on, first, with time cell of normal saline flushing, then add 3mL mixing enzyme solutions afterwards, it is described
It is 1 in volume by 0.25% pancreatin and 0.04% EDTA to mix enzyme solutions:Configuration is formed in the case of 1, is digested, when in microscope
Down it was observed that obvious deformation occurs for cell, shorten into it is spherical after, stop digestion with isometric culture medium immediately, and light with suction pipe
Bottom of bottle portion is beaten in featheriness, it is ensured that cell is completely fallen off into suspension, 1000r/min, centrifuge 5min, then, by centrifuge obtain it is thin
Born of the same parents group is resuspended, and 1 is pressed after adjustment density:2 are inoculated with；1-6, in 37 DEG C, 5% CO2Under the conditions of Secondary Culture；1-7, cell freeze
Deposit, liquid is changed in progress in 12h before freeze-stored cell, and specific steps are identical with step 1-5, that is, discard nutrient solution, physiology salt washing one
Time, digestive juice is added, is waited after the completion of digesting, stops digestion；Gently piping and druming makes cell be suspended in physiological saline, 1000r/min,
Centrifuge 5min；Supernatant is abandoned, then is washed one time with physiology salt, is counted；It is eventually adding serum-free L- in frozen stock solution, described frozen stock solution
DMEM nutrient solutions：Human serum albumin：DMSO=5:4:1 so that cell density is 1 × 106Individual/mL or so；Dispense to 2mL and freeze
Guan Zhong, often pipe 1mL-1.5mL；Cryopreservation tube is put into program temperature reduction box, -80 DEG C of refrigerator overnights is then placed in, goes to liquid nitrogen container
Interior long term storage；1-8, cell recovery, it is rapid from liquid nitrogen to take out cryopreservation tube, it is immediately placed in 37 DEG C of thermostat water baths, not
It is disconnected to rock, it is dissolved completely；Under aseptic condition, cell is transferred in the L-DMEM medium centrifugal pipes of serum-free containing 5mL,
In the case of 1000r/min, 5min is centrifuged, supernatant is abandoned；Repetition is washed one time；Add after appropriate non-animal source serum L-DMEM nutrient solutions
It is inoculated in blake bottle；37℃、5%CO2Under the conditions of cultivate, it is stand-by；Step 2: fat cell is obtained, 2-1, liposuction originated
Adipose tissue normal saline flushing, removes the haemocyte and fragment of tissue of residual, adipose tissue is put into centrifuge tube, records
Initial volume；2-2, isometric digestive juice is added into tissue, described digestive juice is by 0.25% pancreatin, 0.1% type i collagen
Enzyme, with 1:1 ratio mixed preparing is formed, and is put into 37 DEG C of gas bath oscillators and is digested 30min, then stands the tissue digested
5min, after after its layering, draws the fat cell positioned at suspension middle level with suction pipe, is filtered with cell screen clothes, and by after filtering
Cell suspension discards lower floor's suspension with 1000r/min, after centrifugation 2min and obtains fat cell；2-3, suspended with physiological saline it is thin
Born of the same parents, count；Step 3: transplanting, 3-1, the fat stem cell that harvest is cultivated while fat cell is handled, are counted；3-2, general
Fat stem cell presses 2 with fat cell:1 ratio is mixed, and 3-3, is transplanted the cell suspension obtained in step 3-2
To corresponding site.Beneficial effects of the present invention：The obtaining step of fat stem cell is relatively simple, and fat stem cell can be secreted many
The growing nutrient factor is planted, improves the microenvironment inside histoorgan, therefore, is moved altogether with fat cell using autologous fat stem cell
The death caused after fat cell filling due to a lack of nutrient can undoubtedly be substantially improved by planting, so as to improve the transplanting of fat cell
Survival rate.
Embodiment
Present embodiment uses following technical scheme：The method of described raising fat cell transplanting success is：
Step 1: fat stem cell is obtained, 1-1, the adipose tissue that liposuction is originated is put into precooling containing in 1% dual anti-physiological saline, it is close
Under envelope, cryogenic conditions, laboratory is sent back at 2-8 DEG C；1-2, the blood for remaining above-mentioned adipose tissue normal saline flushing, removal
Cell and fragment of tissue, adipose tissue are put into centrifuge tube, record initial volume, isometric digestion is added into tissue
Liquid, described digestive juice is by 0.25% pancreatin, 0.1%I Collagenase Types, with 1:1 ratio mixed preparing is formed, and is put into 37 DEG C of gas baths and is shaken
Swing and 30min is digested in device, the tissue digested is then stood into 5min, now liquid level is divided into 3 layers, and upper strata yellow oil is broken
The fat drips of broken fat cell release, middle level is bulky grain adipose cell layer, or mix a small amount of fiber, lower floor is that single core is thin
Born of the same parents' layer；1-3, upper strata and middle layer cells are discarded, by the cell suspension of lower floor with 1500r/min, abandon supernatant after centrifugation 10min, obtain
To the fat stem cell group of centrifugation bottom of the tube, it is resuspended with non-animal source serum L-DMEM nutrient solutions, density is adjusted to 1 ×
105Individual/mL, is then seeded in culture area for 25cm2Tissue Culture Flask in；1-4, at 37 DEG C 5% CO2Under the conditions of train
Support；1-5, pass and two passed on by one after fat stem cell covers with blake bottle, first, with time cell of normal saline flushing,
Then 3mL mixing enzyme solutions are added, described mixing enzyme solutions are 1 in volume by 0.25% pancreatin and 0.04% EDTA:1
In the case of configure and form, digest, when observing that obvious deformation occurs for cell under the microscope, shorten into it is spherical after, immediately with etc.
The culture medium of volume stops digestion, and gently blows and beats with suction pipe bottom of bottle portion, it is ensured that cell is completely fallen off into suspension, 1000r/
Min, centrifuges 5min, then, and the cell mass that centrifugation is obtained is resuspended, and 1 is pressed after adjustment density:2 are inoculated with；1-6, at 37 DEG C,
5% CO2Under the conditions of Secondary Culture；Liquid, specific steps and step 1- are changed in progress in 12h before 1-7, cell cryopreservation, freeze-stored cell
5 is identical, that is, discards nutrient solution, and physiology salt is washed one time, is added digestive juice, is waited after the completion of digesting, and stops digestion；Gently piping and druming makes
Cell is suspended in physiological saline, 1000r/min, centrifuges 5min；Supernatant is abandoned, then is washed one time with physiology salt, is counted；Finally plus
Enter serum-free L-DMEM nutrient solutions in frozen stock solution, described frozen stock solution：Human serum albumin：DMSO=5:4:1 so that cell density
For 1 × 106Individual/mL or so；Dispense into 2mL cryopreservation tubes, often pipe 1mL-1.5mL；Cryopreservation tube is put into program temperature reduction box, so
After be put into -80 DEG C of refrigerator overnights, go to long term storage in liquid nitrogen container；1-8, cell recovery, it is rapid from liquid nitrogen to take out cryopreservation tube,
It is immediately placed in 37 DEG C of thermostat water baths, and constantly rocks, it is dissolved completely；Under aseptic condition, cell is transferred to containing 5mL
In serum-free L-DMEM medium centrifugal pipes, in the case of 1000r/min, 5min is centrifuged, supernatant is abandoned；Repetition is washed one time；Add
It is inoculated in after appropriate non-animal source serum L-DMEM nutrient solutions in blake bottle；37℃、5%CO2Under the conditions of cultivate, it is stand-by；Step
2nd, fat cell is obtained, 2-1, the adipose tissue normal saline flushing that liposuction is originated, and removes the haemocyte and tissue of residual
Fragment, adipose tissue is put into centrifuge tube, records initial volume；2-2, isometric digestive juice is added into tissue, it is described
Digestive juice by 0.25% pancreatin, 0.1% type i collagen enzyme, with 1:1 ratio mixed preparing is formed, and is put into 37 DEG C of gas bath oscillators
Middle digestion 30min, then stands 5min by the tissue digested, and after after its layering, the fat positioned at suspension middle level is drawn with suction pipe
Fat cell, is filtered with cell screen clothes, and by the cell suspension after filtering with 1000r/min, lower floor's suspension is discarded after centrifuging 2min
Obtain fat cell；2-3, physiological saline suspension cell is used, counted；Step 3: transplanting, 3-1, handle fat cell while
The fat stem cell of culture is harvested, is counted；3-2, fat stem cell and fat cell pressed 2:1 ratio is mixed, 3-3,
The cell suspension obtained in step 3-2 is carried out to be transplanted to corresponding site.The general principle of the present invention has been shown and described above
With principal character and advantages of the present invention.It should be understood by those skilled in the art that, the present invention is not limited to the above embodiments,
Merely illustrating the principles of the invention described in above-described embodiment and specification, is not departing from the premise of spirit and scope of the invention
Under, various changes and modifications of the present invention are possible, and these changes and improvements all fall within the protetion scope of the claimed invention.This hair
Bright claimed scope is by appended claims and its equivalent thereof.
Claims (1)
1. a kind of method for improving fat cell transplanting success, it is characterised in that：Improve the side of fat cell transplanting success
Method is：Step 1: fat stem cell is obtained, 1-1, the adipose tissue that liposuction is originated is put into precooling containing 1% dual anti-physiological saline
In, seal, under cryogenic conditions, laboratory is sent back at 2-8 DEG C；1-2, by above-mentioned adipose tissue normal saline flushing, remove residual
The haemocyte and fragment of tissue stayed, adipose tissue is put into centrifuge tube, records initial volume, is added in equal volume into tissue
Digestive juice, described digestive juice is by 0.25% pancreatin, 0.1%I Collagenase Types, with 1:1 ratio mixed preparing is formed, and is put into 37 DEG C of gas
30min is digested in bath oscillator, the tissue digested is then stood into 5min, now liquid level is divided into 3 layers, upper strata yellow oil
The fat drips of broken fat cell release, middle level is bulky grain adipose cell layer, or mix a small amount of fiber, lower floor is single
Nucleus layer；1-3, upper strata and middle layer cells are discarded, by the cell suspension of lower floor with 1500r/min, abandoned after centrifugation 10min
Clearly, obtain centrifuging the fat stem cell group of bottom of the tube, be resuspended with non-animal source serum L-DMEM nutrient solutions, density is adjusted to
1×105Individual/mL, is then seeded in culture area for 25cm2Tissue Culture Flask in；1-4, at 37 DEG C 5% CO2Under the conditions of
Culture；1-5, pass and two passed on by one after fat stem cell covers with blake bottle, it is first, thin one time with normal saline flushing
Born of the same parents, then add 3mL mixing enzyme solutions, described mixing enzyme solutions by 0.25% pancreatin and 0.04% EDTA volume for
1:Configuration is formed in the case of 1, is digested, when observing that obvious deformation occurs for cell under the microscope, shorten into it is spherical after, use immediately
Isometric culture medium stops digestion, and gently blows and beats with suction pipe bottom of bottle portion, it is ensured that cell is completely fallen off into suspension, 1000r/
Min, centrifuges 5min, then, and the cell mass that centrifugation is obtained is resuspended, and 1 is pressed after adjustment density:2 are inoculated with；1-6, at 37 DEG C,
5% CO2Under the conditions of Secondary Culture；Liquid, specific steps and step 1- are changed in progress in 12h before 1-7, cell cryopreservation, freeze-stored cell
5 is identical, that is, discards nutrient solution, and physiology salt is washed one time, is added digestive juice, is waited after the completion of digesting, and stops digestion；Gently piping and druming makes
Cell is suspended in physiological saline, 1000r/min, centrifuges 5min；Supernatant is abandoned, then is washed one time with physiology salt, is counted；Finally plus
Enter serum-free L-DMEM nutrient solutions in frozen stock solution, described frozen stock solution：Human serum albumin：DMSO=5:4:1 so that cell density
For 1 × 106Individual/mL or so；Dispense into 2mL cryopreservation tubes, often pipe 1mL-1.5mL；Cryopreservation tube is put into program temperature reduction box, so
After be put into -80 DEG C of refrigerator overnights, go to long term storage in liquid nitrogen container；1-8, cell recovery, it is rapid from liquid nitrogen to take out cryopreservation tube,
It is immediately placed in 37 DEG C of thermostat water baths, and constantly rocks, it is dissolved completely；Under aseptic condition, cell is transferred to containing 5mL
In serum-free L-DMEM medium centrifugal pipes, in the case of 1000r/min, 5min is centrifuged, supernatant is abandoned；Repetition is washed one time；Add
It is inoculated in after appropriate non-animal source serum L-DMEM nutrient solutions in blake bottle；37℃、5%CO2Under the conditions of cultivate, it is stand-by；
Step 2: fat cell is obtained, 2-1, the adipose tissue normal saline flushing that liposuction is originated, the blood for removing residual are thin
Born of the same parents and fragment of tissue, adipose tissue are put into centrifuge tube, record initial volume；2-2, isometric digestion is added into tissue
Liquid, described digestive juice is by 0.25% pancreatin, 0.1% type i collagen enzyme, with 1:1 ratio mixed preparing is formed, and is put into 37 DEG C of gas baths
30min is digested in oscillator, the tissue digested is then stood into 5min, after after its layering, is drawn and is located in suspension with suction pipe
The fat cell of layer, is filtered with cell screen clothes, and by the cell suspension after filtering with 1000r/min, is discarded down after centrifuging 2min
Layer suspension obtains fat cell；2-3, physiological saline suspension cell is used, counted；Step 3: transplanting, 3-1, processing fat cell
While harvest culture fat stem cell, count；3-2, fat stem cell and fat cell pressed 2:1 ratio is mixed；
3-3, by the cell suspension obtained in step 3-2 carry out be transplanted to corresponding site.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710402513.9A CN107287155A (en) | 2017-06-01 | 2017-06-01 | A kind of method for improving fat cell transplanting success |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710402513.9A CN107287155A (en) | 2017-06-01 | 2017-06-01 | A kind of method for improving fat cell transplanting success |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107287155A true CN107287155A (en) | 2017-10-24 |
Family
ID=60095412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710402513.9A Pending CN107287155A (en) | 2017-06-01 | 2017-06-01 | A kind of method for improving fat cell transplanting success |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107287155A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108176663A (en) * | 2017-12-28 | 2018-06-19 | 山西阳光中天医疗器械有限公司 | A kind of fat chylification auto-cleaning method |
| CN109566600A (en) * | 2018-12-29 | 2019-04-05 | 中国医学科学院整形外科医院 | It is a kind of to freeze pre-treating method for fat stem cell |
| CN112451745A (en) * | 2019-09-09 | 2021-03-09 | 上海泉眼生物科技有限公司 | High-survival-rate transplantation granular fat and preparation method thereof |
| CN112823799A (en) * | 2019-11-18 | 2021-05-21 | 安迪博斯生命医学研发(天津)有限公司 | Preparation method and application of pharmaceutical composition for treating knee osteoarthritis |
| CN113201489A (en) * | 2021-05-14 | 2021-08-03 | 达瑟儿(上海)生命科技有限公司 | Preparation method of adipose-derived mesenchymal stem cell working cell bank |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058905A (en) * | 2009-11-18 | 2011-05-18 | 四川大学 | Composite fat granule and preparation method thereof |
-
2017
- 2017-06-01 CN CN201710402513.9A patent/CN107287155A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102058905A (en) * | 2009-11-18 | 2011-05-18 | 四川大学 | Composite fat granule and preparation method thereof |
Non-Patent Citations (4)
| Title |
|---|
| LAURA FRESE ET AL.: "Adipose tissue-derived stem cells in regenerative medicine", 《TRANSFUS MED HEMOTHER》 * |
| PURRR: "人来源脂肪干细胞的培养", 《HTTPS://BBS.ANTPEDIA.COM/THREAD-761014-1-1.HTML》 * |
| 罗旋等: "脂肪干细胞应用于脂肪移植的新进展", 《组织工程与重建外科杂志》 * |
| 贾玉磊: "人脂肪干细胞协同颗粒脂肪移植的实验研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108176663A (en) * | 2017-12-28 | 2018-06-19 | 山西阳光中天医疗器械有限公司 | A kind of fat chylification auto-cleaning method |
| CN109566600A (en) * | 2018-12-29 | 2019-04-05 | 中国医学科学院整形外科医院 | It is a kind of to freeze pre-treating method for fat stem cell |
| CN112451745A (en) * | 2019-09-09 | 2021-03-09 | 上海泉眼生物科技有限公司 | High-survival-rate transplantation granular fat and preparation method thereof |
| CN112823799A (en) * | 2019-11-18 | 2021-05-21 | 安迪博斯生命医学研发(天津)有限公司 | Preparation method and application of pharmaceutical composition for treating knee osteoarthritis |
| CN113201489A (en) * | 2021-05-14 | 2021-08-03 | 达瑟儿(上海)生命科技有限公司 | Preparation method of adipose-derived mesenchymal stem cell working cell bank |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107287155A (en) | A kind of method for improving fat cell transplanting success | |
| KR100494265B1 (en) | Composition for treatment of articular cartilage damage | |
| CN106421920A (en) | Fat filler and preparation method thereof | |
| CN102058905B (en) | Composite fat granule and preparation method thereof | |
| CN104498433B (en) | The extracting method and preparation of a kind of fat stem cell and application | |
| CN107384857A (en) | The cultural method of autologous fat mescenchymal stem cell and the culture medium used | |
| CN1912109B (en) | Structural method and application of tissue engineering adipose tissue | |
| CN106727706A (en) | A kind of preparation method of the cell preparation repaired for skin injury | |
| CN108865986B (en) | Mesenchymal stem cell preparation for repairing articular cartilage damage/defect and preparation method and application thereof | |
| CN108309921A (en) | A kind of method for preparing freeze-dried powder rich in cell factor | |
| CN109699634A (en) | A kind of Cryopreservation and method for resuscitation of mescenchymal stem cell | |
| CN110038165A (en) | A kind of enrichment high activity fatty granule cell and the fat graft of fat stem cell and its preparation method and application | |
| CN102284082A (en) | Facial fibrous protein composite filled and positioned in subcutaneous soft tissues, and preparation method thereof | |
| CN109998992B (en) | Treat stem cell injection liquid and preparation method thereof by the umbilical artery of ischemic disease | |
| CN104622772B (en) | A kind of medical cosmetology product of the endothelial progenitor cells containing autologous peripheral blood | |
| CN102258810A (en) | Preparation method of adipose tissue breast augmentation material enriched with autologous stem cells | |
| KR101719743B1 (en) | Method for obtaining stromal vascular fraction from adipose tissue | |
| CN106924814A (en) | The construction method of one boar source collagem membrane Autologous Chondrocyte compound rest | |
| CN108096053A (en) | A kind of application of highly enriched growth factor | |
| CN106995795A (en) | Cellular processes, kit and freeze-dried powder based on platelet rich plasma | |
| CN106983915B (en) | Silk microfibre-gelatin-polyethylene glycol medical catheter and preparation method thereof | |
| CN111544453B (en) | Mixed stem cell injection containing three angiogenesis attributes and preparation method thereof | |
| CN107446889A (en) | A kind of method of umbilical hemopoietic stem cell separation and storage | |
| CN111840643A (en) | Extraction method of active factors for improving survival rate of transplanted autologous fat such as autologous fat living cells | |
| CN106701674A (en) | Purification and extraction process of resuscitation-promoting factors capable of increasing survival rate of transplanted adipocytes or filled adipocytes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171024 |