CN106983910A - A kind of preparation method of polylactic acid composite biological glass tissue repair materials - Google Patents

A kind of preparation method of polylactic acid composite biological glass tissue repair materials Download PDF

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Publication number
CN106983910A
CN106983910A CN201710401396.4A CN201710401396A CN106983910A CN 106983910 A CN106983910 A CN 106983910A CN 201710401396 A CN201710401396 A CN 201710401396A CN 106983910 A CN106983910 A CN 106983910A
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CN
China
Prior art keywords
solution
preparation
polylactic acid
repair materials
tissue repair
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Pending
Application number
CN201710401396.4A
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Chinese (zh)
Inventor
王吉鹏
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Hefei Create Technology Co Ltd
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Hefei Create Technology Co Ltd
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Priority to CN201710401396.4A priority Critical patent/CN106983910A/en
Publication of CN106983910A publication Critical patent/CN106983910A/en
Pending legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/40—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material
    • A61L27/44—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix
    • A61L27/46—Composite materials, i.e. containing one material dispersed in a matrix of the same or different material having a macromolecular matrix with phosphorus-containing inorganic fillers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/56—Porous materials, e.g. foams or sponges
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/58—Materials at least partially resorbable by the body
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/02—Materials or treatment for tissue regeneration for reconstruction of bones; weight-bearing implants

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Transplantation (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Dermatology (AREA)
  • Medicinal Chemistry (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • General Health & Medical Sciences (AREA)
  • Epidemiology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Composite Materials (AREA)
  • Engineering & Computer Science (AREA)
  • Materials Engineering (AREA)
  • Dispersion Chemistry (AREA)
  • Materials For Medical Uses (AREA)

Abstract

A kind of preparation method of polylactic acid composite biological glass tissue repair materials, belongs to biological medicine Material Field, including：Repair materials are made in the preparation of PLA solution, the preparation of bio-vitric solution, electrostatic spinning.Wherein PLA can improve the mechanical performance of material, and bio-vitric can improve the medicinal properties of material.Tissue renovation material produced by the present invention has good mechanical performance, tissue repair performance and drug attachment.

Description

A kind of preparation method of polylactic acid composite biological glass tissue repair materials
Technical field
The invention belongs to biological medicine Material Field, more particularly to a kind of polylactic acid composite biological glass tissue repair materials Preparation method.
Background technology
So far, people's more attention is influence of the bio-vitric component to bioactivity to self-discovery bio-vitric.Nanometer The rise of technology, makes people start to recognize that structure also has a major impact bioactivity, more pays attention to dimensional effect, microcosmic Influence of the fine structure to material structure and performance.Nanometer biological glass, mesoporous biological from nano-scale R ＆ D design Glass is even more the concern for enjoying domestic and foreign scholars.Bio-vitric energy active cell gene, is conducive to strengthening the propagation of cell with dividing Change, the effect with self-bone grafting and osteoacusis.But in view of the presence of its amorphous silicon skeleton, bio-vitric is still rendered as amorphous State, bad mechanical property, and drug attachment is poor, its application on biological medicine material need further exploitation.
The content of the invention
In order to solve problems of the prior art, the invention provides a kind of good mechanical property, and with excellent The preparation method of the polylactic acid composite biological glass tissue repair materials of drug attachment.
The present invention uses following technical scheme：
A kind of preparation method of polylactic acid composite biological glass tissue repair materials, comprises the following steps：
Step one：Particles of polylactic acid is dissolved in chloroform, 6.5wt% solution is configured to, ultrasonically treated 30 minutes, 24 hours are stirred at room temperature until PLA all dissolves, and are added the absolute ethyl alcohol of volume fraction 35%, are stirred at room temperature 24 Hour is uniform to solution clarification, obtains PLA solution；
Step 2：By 5g PEOs-PPOX-, that PEO triblock copolymer is dissolved in 62g volumes is dense Spend in the ethanol solution for 70%, after being sufficiently stirred for, sequentially add 6g silester, 0.7g triethyl phosphates, 1.5gCa (NO3)2·4H2O, 1g0.5M hydrochloric acid solution, obtain mixed liquor；
Step 3：Mixed liquor is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, biology is made Glass solution；
Step 4：By PLA solution and bio-vitric solution in mass ratio 50:8 are well mixed, and use ultrasonic oscillation It is disperseed, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir 8h at room temperature, obtained viscous solution and be placed in and high pressure In the medical needle tubing of device connection, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, by obtained material It is dried in vacuo at 40 DEG C.
It is preferred that, the capacity of the medical needle tubing described in step 4 is 20ml.
It is preferred that, the syringe needle of the medical needle tubing described in step 4 is fixed at electrostatic spinning receiver board 20cm.
The beneficial effects of the present invention are：
1) PLA has good biodegradability, while also having good Bioabsorbable and bio-compatible Property, any environmental issue will not be left after degradation, and in medical field, oneself is considered as most promising degradable macromolecule material Material.
2) bio-vitric has good biocompatibility, and the function with tissue repair, and energy active cell gene has Propagation and differentiation beneficial to enhancing cell, the effect with self-bone grafting and osteoacusis.
3) PLA can improve the mechanical performance of material, and bio-vitric can improve the medicinal properties of material.
4) material prepared using electrostatic spinning technique, with good porosity, is conducive to the attachment of medicine.
Embodiment
With reference to embodiment, the present invention will be further described.
Embodiment 1
A kind of preparation method of polylactic acid composite biological glass tissue repair materials, comprises the following steps：
Step one：Particles of polylactic acid is dissolved in chloroform, 6.5wt% solution is configured to, ultrasonically treated 30 minutes, 24 hours are stirred at room temperature until PLA all dissolves, and are added the absolute ethyl alcohol of volume fraction 35%, are stirred at room temperature 24 Hour is uniform to solution clarification, obtains PLA solution；
Step 2：By 5g PEOs-PPOX-, that PEO triblock copolymer is dissolved in 62g volumes is dense Spend in the ethanol solution for 70%, after being sufficiently stirred for, sequentially add 6g silester, 0.7g triethyl phosphates, 1.5gCa (NO3)2·4H2O, 1g0.5M hydrochloric acid solution, obtain mixed liquor；
Step 3：Mixed liquor is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, biology is made Glass solution；
Step 4：By PLA solution and bio-vitric solution in mass ratio 50:8 are well mixed, and use ultrasonic oscillation It is disperseed, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir 8h at room temperature, obtained viscous solution and be placed in and high pressure In the medical needle tubing of device connection, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, by obtained material It is dried in vacuo at 40 DEG C.
The capacity of medical needle tubing described in step 4 is 20ml；The syringe needle of described medical needle tubing is fixed on apart from electrostatic At spinning receiver board 20cm.

Claims (3)

1. a kind of preparation method of polylactic acid composite biological glass tissue repair materials, it is characterised in that comprise the following steps：
Step one：Particles of polylactic acid is dissolved in chloroform, 6.5wt% solution is configured to, ultrasonically treated 30 minutes, in room temperature Lower stirring adds the absolute ethyl alcohol of volume fraction 35%, is stirred at room temperature 24 hours for 24 hours until PLA all dissolvings It is uniform to solution clarification, obtain PLA solution；
Step 2：5g PEO-PPOX-PEO triblock copolymers are dissolved in into 62g volumetric concentrations is In 70% ethanol solution, after being sufficiently stirred for, 6g silester, 0.7g triethyl phosphates, 1.5gCa (NO are sequentially added3)2· 4H2O, 1g0.5M hydrochloric acid solution, obtain mixed liquor；
Step 3：Mixed liquor is stirred at room temperature after 24h and stood, when solution is in thick solution, that is, bio-vitric is made Solution；
Step 4：By PLA solution and bio-vitric solution in mass ratio 50:8 are well mixed, using ultrasonic oscillation to it Disperseed, magnetic agitation 1h under the conditions of 50 DEG C, then continue to stir 8h at room temperature, obtained viscous solution and be placed in and high-pressure installation In the medical needle tubing of connection, repair materials are made by electrostatic spinning, high-pressure installation voltage is 20kV, by obtained material 40 It is dried in vacuo at DEG C.
2. a kind of preparation method of polylactic acid composite biological glass tissue repair materials according to claim 1, its feature It is：The capacity of medical needle tubing described in step 4 is 20ml.
3. a kind of preparation method of polylactic acid composite biological glass tissue repair materials according to claim 1, its feature It is：The syringe needle of medical needle tubing described in step 4 is fixed at electrostatic spinning receiver board 20cm.
CN201710401396.4A 2017-05-31 2017-05-31 A kind of preparation method of polylactic acid composite biological glass tissue repair materials Pending CN106983910A (en)

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CN201710401396.4A CN106983910A (en) 2017-05-31 2017-05-31 A kind of preparation method of polylactic acid composite biological glass tissue repair materials

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Publications (1)

Publication Number Publication Date
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109594195A (en) * 2019-01-25 2019-04-09 蓝怡科技集团股份有限公司 A kind of cotton shape fibrous framework and its preparation method and application
CN111617320A (en) * 2020-04-20 2020-09-04 广州迈普再生医学科技股份有限公司 Bone induction regeneration membrane and preparation method and application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096262A (en) * 2013-04-09 2014-10-15 上海交通大学医学院附属第九人民医院 Collagen/chitosan/mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof
CN104096261A (en) * 2013-04-09 2014-10-15 上海交通大学医学院附属第九人民医院 Gelatin / mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof
CN104120500A (en) * 2013-04-23 2014-10-29 上海交通大学医学院附属第九人民医院 Fish collagen/mesoporous bioglass composite nanofiber membrane and making method thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104096262A (en) * 2013-04-09 2014-10-15 上海交通大学医学院附属第九人民医院 Collagen/chitosan/mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof
CN104096261A (en) * 2013-04-09 2014-10-15 上海交通大学医学院附属第九人民医院 Gelatin / mesoporous bioactive glass composite nanofiber membrane material and preparation method thereof
CN104120500A (en) * 2013-04-23 2014-10-29 上海交通大学医学院附属第九人民医院 Fish collagen/mesoporous bioglass composite nanofiber membrane and making method thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109594195A (en) * 2019-01-25 2019-04-09 蓝怡科技集团股份有限公司 A kind of cotton shape fibrous framework and its preparation method and application
CN109594195B (en) * 2019-01-25 2021-11-05 蓝怡科技集团股份有限公司 Cotton-shaped fiber scaffold and preparation method and application thereof
CN111617320A (en) * 2020-04-20 2020-09-04 广州迈普再生医学科技股份有限公司 Bone induction regeneration membrane and preparation method and application thereof

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Application publication date: 20170728