CN106978488A - A kind of gene tester, detection kit and its application - Google Patents
A kind of gene tester, detection kit and its application Download PDFInfo
- Publication number
- CN106978488A CN106978488A CN201710215118.XA CN201710215118A CN106978488A CN 106978488 A CN106978488 A CN 106978488A CN 201710215118 A CN201710215118 A CN 201710215118A CN 106978488 A CN106978488 A CN 106978488A
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- China
- Prior art keywords
- test solution
- pipe
- gene
- extraction tube
- detection
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Abstract
The invention discloses a kind of gene tester, detection kit and its application, detection kit includes detection reagent box body, on detection reagent box body pipe is laid provided with test solution, the top that the test solution lays pipe is equipped with the rubber mouth of pipe, the centre of the rubber mouth of pipe is provided with the mouth of pipe, the side for laying pipe in test solution is provided with graduated scale, the bottom that test solution lays pipe is coniform, laid in test solution in pipe and be placed with test solution extraction tube, the top of test solution extraction tube is provided with extraction tube block, the bottom of test solution extraction tube is also coniform, whole detection reagent box body uses safety glass material, detected using this detection kit, detection method reagent is simple, it is cheap, and only contain most basic primer and probe in reaction system, influence factor is few, the inventive method detection is accurate, it is simple to operate, laid a good foundation for quick detection carcinoma of urinary bladder on clinical medicine.
Description
Technical field
The present invention relates to gene tester and detection kit applied technical field, specially a kind of genetic test side
Method, detection kit and its application.
Background technology
Gene is the One function fragment on DNA molecular, is the base unit of hereditary information, is to determine all living things species
The most basic factor；The birth and old age, sickness and death of gene decider are healthy, beautiful, long-lived because being operator and the regulation and control of life
Person.Therefore, where where is it life, just have gene, the form of presence and the decline of all life is all by gene-determined, bag
Include your appearance, height, body weight, the colour of skin, personality etc. inseparable with gene.
Genetic test is the technology detected by blood, other body fluid or cell to DNA, is to take detected person to come off
Oral Mucosal Cells or other histocytes, expand after its gene information, by particular device in detected person's cell
DNA molecular information detects that the risk that precognition body is suffered from the disease analyzes the various genetic profiles contained by it, so that people
The gene information of oneself is will appreciate that, so that living environment and habits and customs by improving oneself, it is to avoid or delay the hair of disease
It is raw.
Genetic test can diagnose the illness, and can be used for the prediction of disease risks.Medical diagnosis on disease is to use genetic test skill
Art detects the mutator for causing genetic disease.Current most widely used genetic test is the inspection of neonate's genetic disease
The auxiliary diagnosis of survey, the diagnosis of genetic disease and some common diseases.There is more than 1000 kinds of genetic disease can be by gene at present
Detection technique makes diagnosis.
Predictive genetic test finds that the pathogenetic risk of disease using technique of gene detection before disease generation, ahead of time
Prevent or take effective intervening measure.There is more than 20 kinds of disease to be predicted with the method for genetic test at present.
When detection, first the gene of person under inspection is extracted from blood or other cells.Then use can be recognized
This portion gene is replicated many times by the primer and round pcr that there may be the gene of mutation, with the mutation for having special marking thing
Gene probe method, enzymatic cleavage methods, gene order detection method etc. judge this portion gene with the presence or absence of mutation or existed sensitive
Genotype.The present invention is relevant with genetic test, especially with regard to a kind of gene tester, detection kit and its application.
The content of the invention
It is an object of the invention to provide a kind of gene tester, detection kit and its application, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides following technical scheme：A kind of gene detecting kit, including detection reagent
Box body, the detection reagent box body lays pipe provided with test solution, and the top that the test solution lays pipe is provided with the rubber mouth of pipe, institute
State test solution and lay the inside of pipe provided with test solution extraction tube.
It is preferred that, the bottom that the test solution lays pipe is coniform, and the tube wall side of the test solution placement pipe, which is provided with, carves
Anale settting scale.
It is preferred that, the centre of the rubber mouth of pipe is provided with the mouth of pipe.
It is preferred that, the top of the test solution extraction tube is provided with extraction tube block, and the extraction tube block uses soft silica gel
Make hollow shape.
It is preferred that, the reagent box body includes the fluorescence labeling probe for TERT genes, the fluorescence labeling probe
Including donor probe and acceptor probe.
It is preferred that, the donor probe and acceptor probe use phosphorating treatment.
It is preferred that, application of the kit in genetic test.
It is preferred that, a kind of gene tester comprises the following steps：
A, oral cavity is cleaned by clear water first；
B, taking-up test solution extraction tube, the physiological saline in pipe are deposited on the bottom of test solution extraction tube；
C, using collection vessel the maxilla cell extraction in oral cavity is come out, the cell of extraction is then put into physiological saline
In, observation pipe internal liquid color from it is original it is limpid be changed into muddy, sample is that collection terminates；
D, by test solution extraction tube the reagent of genetic test is sucked in test solution extraction tube, extracted good cell is carried
Take liquid to draw part to instill in gene detection reagent, by jiggling, cell extract is carried out with gene detection reagent anti-
Should；
E, make sample gene and be complementary to gene to be checked and mixed and hybridized with the DNA probe of fluorescence labeling, use
Gene sequencing, Genotyping, restriction enzyme digestion and electrophoresis method and gene chip detection method are detected to gene.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) in the present invention, whole detection reagent box body uses safety glass material, utilizes the saturating of safety glass material
Bright feature, can intuitively observe the change of liquid color in detection kit；
(2) present invention is detected using kit, and detection method reagent is simple, cheap, and in reaction system
Only contain most basic primer and probe, influence factor is few, the inventive method detection is accurate, simple to operate, is on clinical medicine
Quick detection carcinoma of urinary bladder is laid a good foundation.
Brief description of the drawings
Fig. 1 is overall structure diagram of the invention.
In figure：1st, detection reagent box body；2nd, test solution lays pipe；3rd, test solution extraction tube；4th, the rubber mouth of pipe；5th, lid is extracted
Cap；6th, the mouth of pipe；7th, graduated scale.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme progress applied to the present invention in example is clear, complete
Ground is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based on this
Embodiment in invention, the every other reality that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example is applied, the scope of protection of the invention is belonged to.
Referring to Fig. 1, the present invention provides a kind of technical scheme：A kind of genetic test detection kit, including detection reagent
Box body 1, using the transparent characteristic of safety glass material, can intuitively observe the change of liquid color in detection kit
Change, detection reagent box body 1 lays pipe 2 provided with test solution, the top that test solution lays pipe 2 is provided with the rubber mouth of pipe 4, the rubber mouth of pipe 4
Centre be provided with the mouth of pipe 6, the inside that test solution lays pipe 2 is provided with test solution extraction tube 3, and the top of test solution extraction tube 3 is provided with extraction tube
Block 5, extraction tube block 5 makes hollow shape using soft silica gel, and it is coniform, test solution placement that test solution, which lays the bottom of pipe 2,
The tube wall side of pipe 2 is provided with graduated scale 7.
In the present invention, reagent box body 1 includes the fluorescence labeling probe for TERT genes, and fluorescence labeling probe includes supplying
Body probe and acceptor probe, donor probe and acceptor probe use phosphorating treatment.
A kind of gene tester comprises the following steps：
A, oral cavity is cleaned by clear water first；
B, taking-up test solution extraction tube 3, the physiological saline in pipe are deposited on the bottom of test solution extraction tube 3；
C, using collection vessel the maxilla cell extraction in oral cavity is come out, the cell of extraction is then put into physiological saline
In, observation pipe internal liquid color from it is original it is limpid be changed into muddy, sample is that collection terminates；
D, by test solution extraction tube 3 reagent of genetic test is sucked in test solution extraction tube 3, by extracted good cell
Extract solution is drawn part and instilled in gene detection reagent, by jiggling, and cell extract is carried out with gene detection reagent
Reaction；
E, make sample gene and be complementary to gene to be checked and mixed and hybridized with the DNA probe of fluorescence labeling, use
Gene sequencing, Genotyping, restriction enzyme digestion and electrophoresis method and gene chip detection method are detected to gene.
The present invention is detected that detection method reagent is simple, cheap, and only contains in reaction system using kit
There is most basic primer and probe, influence factor is few, the inventive method detection is accurate, simple to operate, be quick on clinical medicine
Detection carcinoma of urinary bladder is laid a good foundation.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of gene detecting kit, including detection reagent box body (1), it is characterised in that：The detection reagent box body
(1) pipe (2) is laid provided with test solution, the top that the test solution lays pipe (2) is provided with the rubber mouth of pipe (4), and the test solution lays pipe
(2) inside is provided with test solution extraction tube (3).
2. a kind of gene detecting kit according to claim 1, it is characterised in that：The test solution lays pipe (2)
Bottom is coniform, and the tube wall side that the test solution lays pipe (2) is provided with graduated scale (7).
3. a kind of gene detecting kit according to claim 1, it is characterised in that：The centre of the rubber mouth of pipe (4)
Provided with the mouth of pipe (6).
4. a kind of gene detecting kit according to claim 1, it is characterised in that：The top of the test solution extraction tube (3)
Portion is provided with extraction tube block (5), and the extraction tube block (5) makes hollow shape using soft silica gel.
5. a kind of gene detecting kit according to claim 1, it is characterised in that：The reagent box body (1) includes
For the fluorescence labeling probe of TERT genes, the fluorescence labeling probe includes donor probe and acceptor probe.
6. a kind of gene detecting kit according to claim 5, it is characterised in that：The donor probe and acceptor probe
Using phosphorating treatment.
7. a kind of gene detecting kit according to claim any one of 1-6, it is characterised in that：The kit is in base
Because of the application in detection.
8. a kind of gene tester, it is characterised in that：Comprise the following steps：
A, oral cavity is cleaned by clear water first；
B, taking-up test solution extraction tube (3), the physiological saline in pipe are deposited on the bottom of test solution extraction tube (3)；
C, using collection vessel the maxilla cell extraction in oral cavity is come out, then the cell of extraction is put into physiological saline,
Observation pipe internal liquid color from it is original it is limpid be changed into muddy, sample is that collection terminates；
D, by test solution extraction tube (3) reagent of genetic test is sucked in test solution extraction tube (3), by extracted good cell
Extract solution is drawn part and instilled in gene detection reagent, by jiggling, and cell extract is carried out with gene detection reagent
Reaction；
E, make sample gene with being complementary to gene to be checked and being mixed and hybridized with the DNA probe of fluorescence labeling, using gene
Sequencing, Genotyping, restriction enzyme digestion and electrophoresis method and gene chip detection method are detected to gene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710215118.XA CN106978488A (en) | 2017-04-02 | 2017-04-02 | A kind of gene tester, detection kit and its application |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710215118.XA CN106978488A (en) | 2017-04-02 | 2017-04-02 | A kind of gene tester, detection kit and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106978488A true CN106978488A (en) | 2017-07-25 |
Family
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710215118.XA Pending CN106978488A (en) | 2017-04-02 | 2017-04-02 | A kind of gene tester, detection kit and its application |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
| CN114410455A (en) * | 2022-04-01 | 2022-04-29 | 深圳市新一仑生物科技有限公司 | Based on gene detection categorised collection detector |
Citations (6)
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|---|---|---|---|---|
| CN101434987A (en) * | 2007-11-16 | 2009-05-20 | 沈阳守正生物技术有限公司 | Gene detecting method |
| CN102534011A (en) * | 2012-01-18 | 2012-07-04 | 曹利民 | Totally-enclosed fast fluorescence detection method and device for target nucleic acid amplification products |
| CN202543205U (en) * | 2012-04-12 | 2012-11-21 | 湖州市妇幼保健院 | PCR (Polymerase Chain Reaction) centrifugal tube |
| CN103103118A (en) * | 2011-11-15 | 2013-05-15 | 厦门万泰沧海生物技术有限公司 | Nucleic acid amplification and detection reaction tube |
| CN203342428U (en) * | 2013-05-23 | 2013-12-18 | 中国水稻研究所 | Multifunctional extraction tube |
| CN204958918U (en) * | 2015-08-25 | 2016-01-13 | 广州维伯鑫生物科技有限公司 | Reaction tube of pre -installation fluorescence PCR reagent |
-
2017
- 2017-04-02 CN CN201710215118.XA patent/CN106978488A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434987A (en) * | 2007-11-16 | 2009-05-20 | 沈阳守正生物技术有限公司 | Gene detecting method |
| CN103103118A (en) * | 2011-11-15 | 2013-05-15 | 厦门万泰沧海生物技术有限公司 | Nucleic acid amplification and detection reaction tube |
| CN102534011A (en) * | 2012-01-18 | 2012-07-04 | 曹利民 | Totally-enclosed fast fluorescence detection method and device for target nucleic acid amplification products |
| CN202543205U (en) * | 2012-04-12 | 2012-11-21 | 湖州市妇幼保健院 | PCR (Polymerase Chain Reaction) centrifugal tube |
| CN203342428U (en) * | 2013-05-23 | 2013-12-18 | 中国水稻研究所 | Multifunctional extraction tube |
| CN204958918U (en) * | 2015-08-25 | 2016-01-13 | 广州维伯鑫生物科技有限公司 | Reaction tube of pre -installation fluorescence PCR reagent |
Non-Patent Citations (1)
| Title |
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| 默克密理博生命科学部: "Amicon®Ultra-15超滤离心管用户指南", 《HTTPS://WWW.DXY.CN/BBS/NEWWEB/PC/POST/20974968?PAGE=2》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109735437A (en) * | 2019-01-28 | 2019-05-10 | 长春长光辰英生物科学仪器有限公司 | It is collected and the vessel and method that handle after a kind of ejection sorting of cell for cell |
| CN114410455A (en) * | 2022-04-01 | 2022-04-29 | 深圳市新一仑生物科技有限公司 | Based on gene detection categorised collection detector |
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Application publication date: 20170725 |
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