CN101434987A - Gene detecting method - Google Patents

Abstract

The invention belongs to the biological engineering field, and particularly relates to a detection method of genes. The invention is characterized by being implemented according to the following way: 1) the mouth is washed with clean water; 2) No. 1 pipe is taken out and swayed lightly so as to cause the physiological saline in the pipe to be deposited at the bottom of the pipe; 3) cells in oral cavity are taken out with a collector; 4) the cells are sent to the physiological saline in step 3); when the liquid in the pipe turns to turbid from clear by observation, the sample collection is finished; and 5) the sample genes are mixed and hybridized with a DNA probe which is complementary to the genes to be detected and marked with fluorescence, and gene sequencing, genotyping, an enzyme electrophoresis method and a gene chip detection method are used for detecting the genes to be detected. The invention has simple operation and accurate detection, and the base fragment is not lost and substituted, etc.

Description

The detection method of gene

Technical field

The invention belongs to bioengineering field, relate in particular to a kind of detection method of gene.

Background technology

Study on gene mutations has become one of focus of current life science, and detection method also develops rapidly thereupon.The mutation type of human cell oncogene for the detection of transgenation, before 1985, utilizes the Southern blotting as mentioned above, can filter out the mutant forms such as disappearance, insertion and frameshit reorganization of gene.For with the non-detectable sudden change of this method, can only use complicated time-consuming determined dna sequence analytical method.Polymerase chain reaction,PCR (polymerasechain reaction, PCR) technology is the major progress in the mutation research, make the detection in Gene Mutation technology that significant progress arranged, the molecular diagnostic techniques of at present nearly all detection in Gene Mutation all is to build on the basis of PCR, and is constantly occurred by the novel method that PCR derives.

Gene sequencing, genotyping technique (snp), restriction enzyme digestion and electrophoresis method, gene chip etc., gene order surveying method is an internationally recognized standard, the most accurately in the above method.Genotyping technique (SNP) detects and famous with ultra-high throughput, and gene chip also has high-throughout benefit, but because unified standard not also at present, and each laboratory is because hardware, software and operator's difference, often causes that repetition rate is not high as a result.Base tends to occur the phenomenons such as losing, substitute of fragment, and the SNP technology is also just powerless this moment.

Summary of the invention

The present invention is intended to overcome the deficiencies in the prior art part and provides a kind of simple to operate, detects the detection method of gene accurately.

For achieving the above object, the present invention is achieved in that

The detection method of gene, can implement as follows:

1) gargles with clear water;

2) take out pipe No. 1, gently get rid of, make the physiological saline in the pipe be deposited on the pipe end;

3) use collection equipment that intraoral cell is taken out;

4) above-mentioned cell is sent in the physiological saline in the step 3); When observing liquid in pipe by limpid when becoming chaos, sample collecting finishes;

5) make sample gene and be complementary to gene to be checked and mix and hybridize, adopt gene sequencing, gene type, restriction enzyme digestion and electrophoresis method and gene chip detection method that above-mentioned gene to be checked is detected with glimmering dna probe with mark.

Phenomenons such as the present invention is simple to operate, detects accurately, and base fragment can not go out active, substitute.

Embodiment

The detection method of gene, can implement as follows:

1) gargles with clear water;

2) take out pipe No. 1, gently get rid of, make the physiological saline in the pipe be deposited on the pipe end;

3) use collection equipment that intraoral cell is taken out;

4) above-mentioned cell is sent in the physiological saline in the step 3); When observing liquid in pipe by limpid when becoming chaos, sample collecting finishes;

5) make sample gene and be complementary to gene to be checked and mix and hybridize, adopt gene sequencing, gene type, restriction enzyme digestion and electrophoresis method and gene chip detection method that above-mentioned gene to be checked is detected with glimmering dna probe with mark.

Claims (1)

1, the detection method of gene is characterized in that: implement as follows:

1) gargles with clear water;

2) take out pipe No. 1, gently get rid of, make the physiological saline in the pipe be deposited on the pipe end;

3) use collection equipment that intraoral cell is taken out;

4) above-mentioned cell is sent in the physiological saline in the step 3); When observing liquid in pipe by limpid when becoming chaos, sample collecting finishes;

5) make sample gene and be complementary to gene to be checked and mix and hybridize, adopt gene sequencing, gene type, restriction enzyme digestion and electrophoresis method and gene chip detection method that above-mentioned gene to be checked is detected with glimmering D N A probe with mark.