CN106977359A

CN106977359A - A kind of preparation method and purposes of pittosporum tobira fruit extract

Info

Publication number: CN106977359A
Application number: CN201710303747.8A
Authority: CN
Inventors: 宋晓凯; 张秀莲
Original assignee: JIANGSU MARINE RESOURCES DEVELOPMENT RESEARCH INSTITUTE (LIANYUNGANG); Huaihai Institute of Techology
Current assignee: JIANGSU MARINE RESOURCES DEVELOPMENT RESEARCH INSTITUTE (LIANYUNGANG); Huaihai Institute of Techology
Priority date: 2017-05-03
Filing date: 2017-05-03
Publication date: 2017-07-25

Abstract

A kind of preparation method of pittosporum tobira fruit extract, it is characterised in that step is as follows：(1) pittosporum tobira fruit is weighed, the pittosporum tobira fruit weighed is mixed with 95% ethanol；(2) refluxing extraction, suction filtration under high temperature, collect extract solution, and filter residue is extracted 3 times, merge extract solution；(3) by the extract solution vacuum-concentrcted in step (2) and dry to constant weight, obtain pittosporum tobira fruit extract；Preparation method of the present invention designs advantages of simple, the effective ingredient for obtaining pittosporum tobira fruit can be efficiently extracted, as in the important source materials such as medicine, foods and cosmetics, the compound for having more than half is sesquiterpenoids, there is stronger fragrance and biological activity sesquiterpenoids more, much there is important biological function and physiologically active, even more with having antibacterial, antiviral, cytokine, immunosupress, antitumor, plant poison, insect hormone, antifeedant for insect isoreactivity.

Description

A kind of preparation method and purposes of pittosporum tobira fruit extract

Technical field

The invention belongs to biological technical field, and in particular to a kind of preparation method and purposes of pittosporum tobira fruit extract.

Background technology

Pittosporum tobira (scientific name：Pittosporum tobira), Chart database.It is grown on China Guangdong, Hainan, Fujian, Zhejiang It is China's traditional Chinese medicine Deng coastal area.The fruit of pittosporum tobira can be used as medicine, the puckery intestines of energy, have certain curative effect to jaundice, ephritis, And have functions that anti-inflammation detumescence, cough-relieving are breathed heavily.Research shows that pittosporum tobira fruit contains triterpene and carotenoidses compound, and contains There are a large amount of palmitic acid and oleic acid.Up to now, 1. there is not yet point of the volatile chemical component about pittosporum tobira fruit extract Analysis research report；2. the research report of the volatile chemical component anti-endotoxin effect about pittosporum tobira fruit extract is also had not seen Road.

The content of the invention

To overcome above-mentioned technical problem, the technical solution adopted by the present invention is as follows：

A kind of preparation method of pittosporum tobira fruit extract, step is as follows：

(1) pittosporum tobira fruit is weighed, the pittosporum tobira fruit weighed is mixed with 95% ethanol；

(2) refluxing extraction, suction filtration under high temperature, collect extract solution, and filter residue is extracted 3 times, merge extract solution；

(3) by the extract solution vacuum-concentrcted in step (2) and dry to constant weight, obtain pittosporum tobira fruit extract.

Preferably, the weight ratio of pittosporum tobira fruit and 95% ethanol is 1 in the step (1)：3, now it is well mixed, weight It is multiple.

Preferably, reflux temperature is 80 degrees Celsius in the step (2), and the time is 3h, reacts at a temperature of this most fully, carries Take at most.

Preferably, step (3) the pittosporum tobira fruit extract active ingredient is C15H24, entitled 1R, 4S, 7S, 11R- The ring of 2,2,4,8- tetramethyl three [5.3.1.0 (4,11)] -8- is dilute, and molecular weight is 204.

Described pittosporum tobira fruit extract is that effective ingredient prepares antitoxic purposes.

Beneficial effect：Preparation method of the present invention designs advantages of simple, can efficiently extract and obtain the main of pittosporum tobira fruit Active ingredient, the molecular formula of its compound is C15H24, entitled 1R, 4S, 7S, 11R-2,2, the ring of 4,8- tetramethyl three [5.3.1.0 (4,11)] -8- is dilute, and molecular weight is 204.From its molecular formula, carbon number is 15, therefore learns this compound for again Half terpene, as in the important source materials such as medicine, foods and cosmetics, the compound for having more than half is sesquiterpenoids, sesquialter There is stronger fragrance and biological activity, much with important biological function and physiologically active, more terpenoid more It is to have antibacterial, antiviral, cytokine, immunosupress, antitumor, plant poison, insect hormone, antifeedant for insect isoreactivity.

Brief description of the drawings

The total ion current figure that Fig. 1 analyzes for the volatile chemical component GC-MS of pittosporum tobira fruit extract；

Fig. 2 is bacterial endotoxin standard curve with adding the curve after 10 times of dilution, 50 times of fruit extracting solutions.

Embodiment

The fruit extractum thing of pittosporum tobira is subjected to ultrasound with methanol, with gas chromatography-mass spectrum (GC-MS) combined instrument to it Supernatant is analyzed, and has obtained following analysis result.

The total ion current figure of GC-MS analyses is as follows：

1. chromatographic condition is as follows：

Chromatographic column：DB-5MS, 30m*0.25mm*0.25um, carrier gas He 1.0ml/min.Column temperature：40 degree of * 3min---10 Degree/min---100 degree --- 6 degree/min---300 degree * 5min

Injection port：280 degree, shunting：5 0:1

Transmission line：280 degree

EI sources：250 degree

m/z：33-650

2. the total ion current figure of the volatile chemical component GC-MS analyses of pittosporum tobira fruit extract is as shown in Figure 1：

3. the volatile chemical component GC-MS analyze datas of pittosporum tobira fruit extract and it the results are shown in Table 1, table 2 (table include The retention time of each chemical composition, peak area (i.e. percentage contents), are kissings with each compound in Fig. 1 above It is closing, consistent).

The volatile chemical component GC-MS analyze datas of the pittosporum tobira fruit extract of table 1

The volatile chemical component GC-MS analysis results of the pittosporum tobira fruit extract of table 2

It was found from GC-MS spectrogram, top is occurred in that when retention time is 40.85min, it can thus be appreciated that this peak correspondence Under compound be pittosporum tobira fruit extract main component, the molecular formula of its compound is C15H24, entitled 1R, 4S, 7S, 11R-2, the ring of 2,4,8- tetramethyl three [5.3.1.0 (4,11)] -8- is dilute, and molecular weight is 204.From its molecular formula, carbon number is 15, therefore learn that this compound is sesquiterpenoids.As in the important source materials such as medicine, foods and cosmetics, there is more than half Compound is sesquiterpenoids.There is stronger fragrance and biological activity, much with important sesquiterpenoids more Biological function and physiologically active, even more with having antibacterial, antiviral, cytokine, immunosupress, antitumor, plant poison, insect Hormone, antifeedant for insect isoreactivity.From GC-MS analysis results, the antitoxin effect of pittosporum tobira fruit extract is effective with it Composition is that sequiterpene is relevant.

Carry out for fruit extracting solution preliminary antitoxin (mainly for induced by lipopolysaccharide) mechanism of action it is preliminary Research：

Dilution 10 times and 50 times of mistakes are added in concentration is 0.1Eu/mL and 0.8Eu/mL bacterial endotoxin standard liquid Fruit extracting solution afterwards, and their absorbance is measured when wavelength is 545nm, it resulting in the following knot being compared to each other Fruit is as shown in Figure 2.

From comparative result, bacterial endotoxin standard liquid is adding fruit extraction of 10 times of the dilution with diluting 50 times After liquid, absorbance has a declining tendency, and the decline of absorbance illustrates that the concentration of bacterial endotoxin in solution declines, because This, we can draw the antitoxin effect of pittosporum tobira fruit extracting solution.

Illustrate the implementation of the technical program with specific embodiment below.

Embodiment 1

A kind of preparation method of pittosporum tobira fruit extract, it is characterised in that step is as follows：

(1) pittosporum tobira fruit 800g is weighed, by the pittosporum tobira fruit weighed and 95% ethanol with 1:3 weight are than mixing；

Refluxing extraction 3h at a temperature of (2) 80 degrees Celsius, suction filtration collects extract solution, and filter residue is extracted 3 times, and merging is carried Take liquid；

Pittosporum tobira fruit extract is that active ingredient can prepare antitoxin.

Claims

1. a kind of preparation method of pittosporum tobira fruit extract, it is characterised in that step is as follows：

2. a kind of preparation method of pittosporum tobira fruit extract according to claim 1, it is characterised in that：The step (1) The weight ratio of middle pittosporum tobira fruit and 95% ethanol is 1：3.

3. a kind of preparation method of pittosporum tobira fruit extract according to claim 1, it is characterised in that：The step (2) Middle reflux temperature is 80 degrees Celsius, and the time is 3h.

4. a kind of preparation method of pittosporum tobira fruit extract according to claim 1, it is characterised in that：The step (3) Pittosporum tobira fruit extract active ingredient is C15H24, entitled 1R, 4S, 7S, 11R-2,2, the ring [5.3.1.0 of 4,8- tetramethyl three (4,11)] -8- is dilute, and molecular weight is 204.

5. the pittosporum tobira fruit extract described in claim 1-4 any one prepares antitoxic purposes for effective ingredient.