CN106967614B

CN106967614B - Cladosporium SCSIO43503 and application thereof

Info

Publication number: CN106967614B
Application number: CN201710103577.9A
Authority: CN
Inventors: 董俊德; 曾思泉; 凌娟; 张燕英; 周卫国; 马卓尔; 林丽云; 王聪
Original assignee: South China Sea Institute of Oceanology of CAS
Current assignee: South China Sea Institute of Oceanology of CAS
Priority date: 2017-02-24
Filing date: 2017-02-24
Publication date: 2020-06-09
Anticipated expiration: 2037-02-24
Also published as: CN106967614A

Abstract

The invention discloses cladosporium SCSIO43503 and application thereof. Cladosporium (Gladaxporism sp.) SCSIO43503 was deposited at the Chinese Collection for Type Culture Collection (CCTCC) on 2016, 11/03, address: china, Wuhan and Wuhan university, the preservation number is CCTCC NO: m2016613. The strain has cellulase production activity and can be used for producing cellulase, thereby having important value for the production and utilization of cellulase. Further can be used for manufacturing biological organic matter degradation bacterial manure.

Description

Cladosporium SCSIO43503 and application thereof

The technical field is as follows:

the invention belongs to the technical field of biology, and particularly relates to cladosporium SCSIO43503 and application thereof.

Background art:

mangrove forest is a halophytic forest plant community on the salty marsh of estuary mud beach of tropical and subtropical gulfs, and is also called as coastal saltation marsh vegetation because the mangrove forest grows in intertidal zones where coastal tides reach and the dryness and the humidity overlap (Wangwangqin et al, 2007; Wangwoshu, 2013). The mangrove is in a saline-fresh water connection zone, and the mangrove wetland retains a large amount of land-source pollutants, so that the mangrove wetland plays an important role in purifying water bodies of estuaries and oceans. The special habitat brings about the functions of pollution resistance and pollution reduction of mangrove plants, so that the mangrove wetland system has long been studied abroad to have the potential of sewage purification like other types of wetlands (Nedwell D.B., 1974; Dwivedi S.N et al, 1983). Mangrove forest is located in the intertidal zone of alternation between dry and wet, and its salty environment makes the redox potential in the ecosystem between-200 and +150mV (Clark MW, 1998). This has led to the abundance of microbial resources in this area, which play an important role in material circulation, energy flow, ecological balance and environmental cleanup, where the diversity and activity of microorganisms underlie the restoration and reconstruction of mangrove ecosystem (Fernando Dini Andreote et al, 2012).

In mangrove ecosystem, plant litter and organic debris are very rich, and there are a large number of microorganisms that can degrade macromolecular organic substances such as cellulose, lignin and chitin (panhu et al, 2012). At present, people have very limited cognition on microbial groups in mangrove ecological systems. The results of culturable separation studies show that: the main groups of microorganisms in mangrove ecosystems are bacteria and fungi, with a low number of actinomycetes and pico-type plankton (Jianyuxia et al, 2006; Gina H et al, 2001). More than one hundred species of mangrove fungi have been isolated and identified as the second major group of marine fungi. In mangrove tropicalis, the composition of the microorganisms is approximately: bacteria and fungi account for 91% of the total microbial resources, and algae and protozoa for 7% and 2%, respectively (Alongi DM, 1988; Zhuang T et al, 1998).

Cellulose is the most abundant renewable resource on earth (several billion tons/year) and is also the primary product of photosynthesis, cellulose is a linear polysaccharide formed by connecting glucose monomers by β -1, 4-glycosidic bonds, cellulase hydrolyzes cellulose into glucose, is widely used in food, feed, textile industry and the like, and has great application potential (B.C. Behera1et al, 2014; B.C. Behera et al, 2016). fungus is generally secreted to the outside of cells and exists in the form of cellulose bodies attached to cell membranes in certain anaerobic fungi, wherein Trichoderma is the most widely studied cellulase producing bacteria, and 20% of cellulase in the world cellulase market is from Trichoderma and Aspergillus (Hanif. 2004; Kang et al, 2004; Aro et al, 2005; Ahamed and Vermette, 2008).

The invention content is as follows:

the first purpose of the invention is to provide a new species of mangrove plant rhizosphere fungi which is screened from rhizosphere soil of tropical mangrove forest in san city, Hainan province of China and has higher cellulose degradation activity: cladosporium (Gladaxporismsp.) SCSIO43503, deposited at the Chinese Collection for Type Culture Collection (CCTCC) on year 2016, 11/03, address: china, wuhan university, accession number: CCTCC NO: m2016613.

The invention relates to a novel high-efficiency cellulose degradation fungus species: a Cladosporium species (Gladaxporism sp.) SCSIO43503 was selected from rhizosphere soil of tropical mangrove forest of Mitsui, Hainan province.

Morphological and physiological biochemical characteristics of cladosporium (Gladaxporism sp.) SCSIO 43503:

strain description: as shown in FIG. 1, the colonies were flat, occasionally solid, gray, velvet, green-black on the back, and had a colorless halo. Conidiophores are cladosporium, conidiophores extend in a shaft-combining mode, conidiophores grow to a certain length, tops of the conidiophores expand to form chain-grown spores, the spores grow on the side face of the expanded top, the spores are generated on new tops, the conidiophores continue to extend, the process is repeated for multiple times, the conidiophores expand in a knee bending mode, and obvious spore marks are left at the expanded positions after the spores fall off. The strain has fine vegetative hyphae, separation and branching and a width of 5.0-10 mu m. No specialized conidiophores, spore-forming cells directly grow on hyphae, the length of the spore-forming cells is 8-35 mu m, the base width of the spore-forming cells is 1.5-3.5 mu m, and the top of the spore-forming cells is less than 1.0 mu m; conidia are oval, rarely nearly cylindrical, and 1.5-3.5X 1.0-2.0 μm. The growth of the colony on the wort agar culture medium is slow, the diameter of the colony is about 30mm under the dark condition of 28 ℃ for 7 days, and the colony has no water-soluble pigment and has obvious resistance to various antibiotics. The strain is inoculated on a flat plate which takes sodium carboxymethylcellulose (CMC-Na) as a main carbon source, and after the strain is cultured for 5 days at the temperature of 30 ℃, a hydrolysis transparent ring with the diameter of 25mm can be formed (figure 1B), which shows that the strain has higher degradation activity of biomass such as cellulose.

Cladosporium (Gladaxporism sp.) SCSIO43503 molecular taxonomic position:

genomic DNA was extracted from a pure culture of Cladosporium (Gladaxporism sp.) SCSIO43503, and the sequence of ITS was obtained by PCR amplification and sequencing analysis using primers ITS1/ITS4 specific to the sequence of rDNA Internal Transcribed Spacer (ITS) and the nucleotide sequence shown in SEQ ID NO. 1. the sequence of β -tubulin was obtained by PCR amplification and sequencing analysis using primers Bt2a/Bt2b specific to the sequence of β tubulin (β -tubulin) and the nucleotide sequence shown in SEQ ID NO. 2. the sequence of calmodulin (calamine) was obtained by PCR amplification and sequencing analysis using primers d5/cmd6 specific to the sequence of calmodulin, the nucleotide sequence shown in SEQ ID NO. 3. the sequence of ITS, the sequence of β -tubulin and the sequence of caldelin were aligned with the BLAST software in GenBank to find that there was no sequence in the database where no gene sequence was found, and no gene sequence was found in the database.

A part of representative gene sequences with higher similarity to determined sequences are selected from GenBank databases, phylogenetic trees are constructed by a Neibar-joining method through Mega software (see figures 2, 3 and 4), and phylogenetic analysis is carried out, i.e. the phylogenetic trees can show that the cladosporium (Gladaxporism sp.) SCSIO43503 has larger difference from known fungi, sequence sequencing analysis of transcribed spacer (ITS) sequences in rDNA shows that the nearest cladosporium strains with the relativity are FJ755255.1 and LN714576.1, which have 96% similarity to the cladosporium (Gladaxporism sp.) SCSIO43503, BLAST analysis of the microtubulin (β -tubulin) sequences shows that the cladosporium (Gladaxporism sp.) SCSIO43503 has only about 83% to 86% similarity to all known microtubulin regions, and the sequence alignment has only about 52% similarity to the calmodulin (Paramercu) 465989%, and the sequence alignment has only about 52% similarity to the known strains.

From the above results of morphology, physiological and biochemical characteristics and molecular taxonomy, we determined that it is a new strain of fungus, which is classified as cladosporium (Gladaxporism sp.), named as cladosporium (Gladaxporism sp.) SCSIO43503, deposited in the chinese typical culture collection (CCTCC) at 2016, 11, 03 days, address: china, wuhan university, accession number: CCTCC NO: m2016613.

Cellulase activity (CMC enzyme activity) studies of cladosporium (Gladaxporism sp.) SCSIO43503 showed: under the conditions of 30 ℃ and pH 7.0, the activity of liquid fermentation cellulase reaches 23.46U (figure 5) in about 3 days, which shows that the cladosporium (Gladaxporism sp.) SCSIO43503 has better biomass degradation activity of cellulose and the like. Therefore, the second objective of the present invention is to provide the application of cladosporium (Gladaxporism sp.) SCSIO43503 in cellulase production.

The third purpose of the invention is to provide an application of cladosporium (Gladaxporism sp.) SCSIO43503 in preparation of the biological organic matter degradation bacterial fertilizer.

The fourth purpose of the invention is to provide a biological organic matter degradation bacterial fertilizer, which is characterized in that Cladosporium (Cladosporium sp.) SCSIO43503 is used as an active ingredient.

The invention provides a new fungus species: the cladosporium (Gladaxporism sp.) SCSIO43503 has cellulase production activity, can be used for producing cellulase, and has important value on the production and utilization of the cellulase. Further can be used for manufacturing biological organic matter degradation bacterial manure.

The cladosporium (Gladaxporism sp.) SCSIO43503 of the present invention was deposited in the chinese typical culture collection center (CCTCC) at 2016, 11, 03 days, address: china, wuhan university, accession number: CCTCC NO: m2016613.

Description of the drawings:

FIG. 1 shows the growth of a Mycospora species (Gladaxporism sp.) SCSIO43503 colony on PDA medium (A), scanning electron micrographs (C and D), and the decolorization of a Mycospora species (Gladaxporism sp.) SCSIO43503 colony on a CMC-Congo red plate (B);

FIG. 2 is a position diagram of a Cladosporium sp (Gladaxporism sp.) SCSIO43503 in an rDNA transcribed spacer (ITS) rootless phylogenetic tree;

FIG. 3 is a positional diagram of a Cladosporium sp (Gladaxporism sp.) SCSIO43503 in β tubulin (β -tubulin) rootless phylogenetic tree;

FIG. 4 is a position diagram of SciO 43503 of Cladosporium sp (Gladaxporism sp.) in a calponin (calmodulin) rootless phylogenetic tree;

FIG. 5 is a graph of the cellulase activity (CMC enzyme activity) of liquid fermentation of Cladosporium sp (Gladaxporism sp.) SCSIO 43503.

The specific implementation mode is as follows:

the following examples are further illustrative of the present invention and are not intended to be limiting thereof.

Example 1:

materials and methods

1 Material

1.1 soil sample Collection

Samples were collected from rhizosphere deposits of red sea olive (Rhizophorastylosa) in the red forest region along the bank in the red sand river, Mitsui, Hainan, 5 months in 2014, and the samples were filled into sterilized sealed polyethylene bags and taken back to the laboratory for low-temperature storage at-20 ℃.

1.2 culture Medium

1.2.1 isolation Medium:

the preparation method of each liter of separation culture medium is as follows: potato juice (potato is peeled, bud eyes are dug, the potato is cleaned and sliced, 200g of the potato juice is weighed and put into 1000mL of tap water, the mixture is boiled for 30min by slow fire, double-layer gauze is used for filtering, and the filtrate is added with water to supplement the volume to 1000mL) by 800 mL; 20.0g of cane sugar; NaCl 1.5 g; 15g of agar; soil leaching liquor (200 mL soil leaching liquor is obtained by uniformly mixing a soil sample, taking 100g soil sample, adding 500mL sterile water, shaking for 15min at 180rpm, standing for 30s, and filtering and sterilizing supernate to obtain the soil leaching liquor); after sterilization at 121 ℃ and the like, the medium was cooled to 60 ℃ and 1mL each of 100. mu.g/mL ampicillin and 100. mu.g/mL streptomycin sulfate sterilized by filtration was added.

1.2.2 preservation Medium:

the preparation method of each liter of preservation culture medium is as follows: potato juice (potato is peeled, bud eyes are dug, the potato is cleaned and sliced, 200g of the potato juice is weighed and put into 1000mL of tap water, the mixture is boiled for 30min by slow fire, double-layer gauze is used for filtering, and the filtrate is added with water to supplement 1000mL) of 1000 mL; 20.0g of cane sugar; NaCl 1.5 g; 15g of agar.

1.2.3 fermentation Medium:

the preparation method of each liter of fermentation medium is as follows: 5.0g of CMC-Na; peptone 1.0 g; 1.0g of yeast extract; 1.0g of cane sugar; KNO₃1.0g；K₂HPO₄0.5g；MgSO₄·7H₂0.5g of O; NaCl 1.5 g; 1000mL of water.

1.2.4 CMC enzyme activity identification culture medium:

the preparation method of the CMC enzyme activity identification culture medium per liter comprises the following steps: 10.0g of CMC-Na; KNO₃1.0g；K₂HPO₄0.5g；MgSO₄·7H₂0.5g of O; NaCl 1.5 g; congo red 0.2 g; 15g of agar; 1000mL of water.

2. Method of producing a composite material

2.1 isolation and selection of strains

Selecting three vigorous radix tinosporae (Rhizophora stylosa) plants from the same sample, collecting their rhizosphere soil samples 50g, mixing the three samples, placing about 5g into a triangular flask containing 50mL sterilized distilled water, shaking at 30 deg.C and 150r/min for 15min, standing for 30sec, taking 1mL of bacterial solution, diluting in gradient, selecting 10^-3、10^-4、10^-5Three dilutions were plated, 0.05mL, on isolation medium in 8 replicates per treatment. Culturing at 30 ℃ for 3-5 days, selecting single colony with typical morphology, and performing point grafting purification for 2-3 times to obtain a pure culture. Inoculating the obtained pure culture to a CMC enzyme activity identification culture medium, carrying out inverted culture for 3 days at 30 ℃, covering Lugol iodine solution on the culture medium on which the bacterial colony grows for 3-5 min, and then pouring out the Lugol iodine solution, wherein a transparent ring appears around the bacterial colony generating the cellulase. And (3) identifying the strain capable of producing the cellulase according to the primary decolorization effect, measuring the diameter of the transparent ring and the diameter of the bacterial colony, and screening the strain with better effect for further research, thereby obtaining a pure strain culture, namely SCSIO43503, and storing the pure strain culture in a storage medium.

2.2 morphological characteristics of the Strain

The morphological characteristics and preliminary identification of the strain are according to the fungal identification handbook and the fourteenth volume of the Chinese journal of fungi.

The SCSIO43503 strain screened in the above steps has the following strain morphology description: as shown in FIG. 1, the colonies were flat, occasionally solid, gray, velvet, green-black on the back, and had a colorless halo. Conidiophores are cladosporium, conidiophores extend in a shaft-combining mode, conidiophores grow to a certain length, tops of the conidiophores expand to form chain-grown spores, the spores grow on the side face of the expanded top, the spores are generated on new tops, the conidiophores continue to extend, the process is repeated for multiple times, the conidiophores expand in a knee bending mode, and obvious spore marks are left at the expanded positions after the spores fall off. The strain has fine vegetative hyphae, separation and branching and a width of 5.0-10 mu m. No specialized conidiophores, spore-forming cells directly grow on hyphae, the length of the spore-forming cells is 8-35 mu m, the base width of the spore-forming cells is 1.5-3.5 mu m, and the top of the spore-forming cells is less than 1.0 mu m; conidia are oval and rarely nearly cylindrical, and the diameter of the conidia is 1.5-3.5 multiplied by 1.0-2.0 mu m. The growth of the colony on the wort agar culture medium is slow, the diameter of the colony is about 30mm under the dark condition of 28 ℃ for 7 days, and the colony has no water-soluble pigment and has obvious resistance to various antibiotics. The strain is inoculated on a culture medium plate which takes sodium carboxymethylcellulose (CMC-Na) as a main carbon source, and after the strain is cultured for 5 days at 30 ℃, a hydrolysis transparent ring with the diameter of 25mm can be formed (figure 1B), which shows that the strain has higher degradation activity of biomass such as cellulose.

2.3 extraction of genomic DNA of Strain and molecular biological identification

The obtained pure culture strain SCSIO43503 was subjected to extraction of total DNA using the Omega fungal genome extraction kit, and then used for amplification of rRNA genes.

Amplification of partial sequences of the fungal ITS regions general primers ITS1/ITS4(T.J.white, T.Bruns et al, 1990), ITS 1: 5'-TCCGTAGGTGAACCTGCGG-3', ITS 4: 5'-TCCTCCGCTTAT TGATATGC-3' are provided.

β -tubulin (β -tubulin) sequence was amplified using the universal primers Bt2a/Bt2b (Glass, Donaldson, 1995), Bt2 a: 5'-GGTAACCAAATCGGTGCTGCTTTC-3', Bt2 b: 5'-ACCCTCAGTGTAGTGACCCTTGGC-3'.

Amplification of calponin (calmodulin) sequences with the universal primers cmd5/cmd6(Seung-Beomhong, Hye-Sun Cho et al, 2006), cmd 5: 5'-CCGAGTACAAGGAGGCCTTC-3', cmd 6: 5'-CCGATAGAGGTCATAACGTGG-3' are provided.

The PCR reaction system is shown in Table 1.

TABLE 1 PCR reaction System

The PCR reaction program comprises pre-denaturation at 95 ℃ for 2min, denaturation at 95 ℃ for 30s, annealing at 55 ℃ for 30s, extension at 72 ℃ for 1min, 35 cycles, extension at 72 ℃ for 7min, taking 5 microliter of PCR product, detecting the product by 1% agarose electrophoresis, purifying the amplified product, sending the product to a sequencing company for sequencing, obtaining an ITS sequence by PCR amplification and sequencing analysis by using an ITS1/ITS4 specific to an rDNA Inner Transcription Spacer (ITS) sequence, wherein the nucleotide sequence is shown as SEQ ID NO.1, obtaining a β -butulin sequence by PCR amplification and sequencing analysis by using a primer Bt2a/Bt2b specific to a β tubulin (β -tubulin) sequence, obtaining a 5635-butulin sequence by PCR amplification and sequencing analysis, wherein the nucleotide sequence is shown as SEQ ID NO.2, using a primer cmd5/cmd6 specific to a calponin (calponin) sequence, obtaining a nucleotide sequence by PCR amplification and sequencing by comparing the nucleotide sequence with a gene sequence shown as SEQ ID NO.3 in a database, and finding that no nucleotide sequence is similar to the gene sequence found in a new gene database by using a cDNA 26.

A representative gene sequence with high similarity to a determined sequence is partially selected from GenBank databases, a phylogenetic tree is constructed by a Neibar-joining method through Mega software (see figures 2, 3 and 4) and phylogenetic analysis is carried out, the strain SCSIO43503 has large difference with known fungi, the sequence sequencing analysis of a transcribed spacer (ITS) in rDNA shows that the Cladosporium strains with the closest relativity are FJ755255.1 and LN714576.1, and the similarity with the SCSIO43503 is only 96 percent, the BLAST analysis of the sequence of β tubulin (β -tubulin) shows that the sequence alignment coverage rate of the SCSIO43503 has only about 83 to 86 percent similarity with the β tubulin region of all known strains, the sequence alignment coverage rate of the calcium regulatory protein (calcimodulin) is only 52 percent, the similarity with the sequence of the Cladosporium maculatum (Disco-located in β tubulin) of all known strains is confirmed by the fact that the strain has the similarity with the Cladosporium strain deposited in Shisanda No. 5, the Glossium strain deposited in the China center No. 7: Glossella Shisanura, the accession No. 7: Glossella typhimurium (Glossinodorsum) and the accession No. 7: Glossinodorsum of the Glossicle accession No. 7. it is determined that the Glossium strain deposited in the Glossa strain deposited in the university of the Glossicle accession No.3, the Glossium strain deposited in the Glossum strain deposited in China.

2.4 determination of cellulase Activity (CMC enzyme Activity)

Inoculating Sclerotinia cladosporioides (Gladaxporism sp.) SCSIO43503 into fermentation medium, and measuring cellulase activity of the fermentation liquid by taking a small amount of fermentation liquid every day at 30 deg.C and 180 rpm/min. The fermentation broth was centrifuged at 6000rpm/min for 10min and the supernatant was used as the crude enzyme solution. Adding 0.5mL of crude enzyme solution and 1.5mL of citric acid buffer solution with pH of 5.0 into a 18cm × 18mm glass test tube, preheating for 5min in a 50 ℃ constant-temperature water bath, adding 1 × 1cm of quantitative filter paper, performing enzymolysis for 30min in a 50 ℃ constant-temperature water bath, adding 2mL of DNS into a boiling water bath for 5min, naturally cooling, extracting supernatant, measuring the light absorption value at 540nm, and measuring the enzyme activity after contrasting a standard curve. The enzyme activity of the fermentation broth is shown in fig. 5, and it can be seen from fig. 5 that the fermentation broth has cellulase activity, the cellulase activity of the fermentation broth gradually increases with the increase of the fermentation time, and the cellulase activity of the fermentation broth reaches 23.46U after 3 days. This indicates that the cladosporium (Gladaxporism sp.) SCSIO43503 of the present invention has cellulase-producing activity.

The enzyme activity is defined as follows according to the international unit regulation: the amount of enzyme required to catalyze the hydrolysis of cellulose to 1. mu. mol glucose per minute is 1 enzyme activity unit U.

Sequence listing

<110> Nanhai ocean institute of Chinese academy of sciences

<120> Cladosporium SCSIO43503 and application thereof

<160>3

<210>1

<211>523

<212>DNA

<213> Cladosporium sp (Gladaxporism sp.) SCSIO43503

<400>1

tagttttctc gggggggctt cggcctctgt ctgacaccct tgtcttttgc gtactatttt 60

gtttccttgg tgggcgtttg cctgccagca ggacactata taaccttttg taattgcaat 120

cagcgtcaga aaaacataaa tttattacaa ctttcaacaa cggatctctt ggttctggca 180

tcgatgaaga acgcagcgaa atgcgaaaag tagtgtgaat tgcagaattc agtgaatcat 240

cgaatctttg aacgcacatt gcgccccttg gtattccatg gggcatgcct gttcgagcgt 300

catttgtacc ttcaagctct gcttggtgtt gggtgtttgt cccgctttct gcgtggactc 360

gcctcaaagc aattggcagc cggcgtacta gcctgggagc gcagcacatt ttgcgtctct 420

cgactggaaa cactggcgtc catgaagcct atactcttgc tcttgacctc ggatcaggta 480

gggatacccg ctgaacttaa gcatatcaaa accgcggagg aaa 523

<210>2

<211>321

<212>DNA

<213> Cladosporium sp (Gladaxporism sp.) SCSIO43503

<400>2

aggtccctct ctggcgagca tggtctcgat ggctctggtg tctacaatgg cacctcggac 60

cttcagctcg agcgtatgaa cgtctatttc aacgaggtac gcgatttctc cctcacgctg 120

tcgaatgagc gggacatact aaccaatggc aggcttctgg caacaagttt gttccccgcg 180

ctgttctcgt tgatcttgag cccggcacaa tggacgctgt ccgtgccggt cctttcggac 240

agctcttccg tcccgacaac ttcgttttcg gtcagtctgg tgctggtaac aactgggcca 300

agggtcacac aactgagggt a 321

<210>3

<211>647

<212>DNA

<213> Cladosporium sp (Gladaxporism sp.) SCSIO43503

<400>3

gggggagggc tcgttcgtct ctgccgtatc ttgctgctct ggtgcctggt tgcctagcag 60

cccatgcgta gtctcctggt acaacaaaca tgctactaac acgaattgct ctcaggacaa 120

ggacggcgat ggtcagtaac ctccccatga gacaggctca cgatgccttt gcgtcgagac 180

tggcaatcgg tgctcagccc gcgcaattga caacggtcga gttcagctga aggacgaggc 240

ttggacgagg ctaaccctcg atgccacccc gcggctgcat cctaatatac taccaaaagc 300

gtattactga ccatgccacc tcacaggtca gatcaccacc aaggaattgg gcacggtcat 360

gcgctccctt ggtcagaacc ccagcgagtc tgagttgcaa gacatgatca acgaggttga 420

tgccgataac aatggcacca ttgattttcc aggtaccgga agacatgtcg tgtaaatttc 480

cctaggttcg cgttaacgac cagtccagaa ttcctcacca tgatggctcg caaaatgaag 540

gacactgatt ccgaggagga gatccgagaa gcgttcaaag tgttcgaccg tgacaacaat 600

ggcttcatct ctgctgctga gctgcgccac gttatgcctt ctatcgg 647

Claims

1. Cladosporium sp SCSIO43503, deposited under the accession number: CCTCC NO: m2016613.

2. Use of the Cladosporium (Cladosporium sp.) SCSIO43503 of claim 1 for producing cellulase.

3. The use of the Cladosporium (Cladosporium sp.) SCSIO43503 of claim 1 for preparing a biological organic matter degrading bacterial fertilizer.

4. A biological organic matter-degrading bacterial fertilizer characterized by comprising Cladosporium sp (Cladosporium sp.) SCSIO43503 as an active ingredient.