CN106967132B - Reactive compound and its preparation method and application in Shenzhou City's honey peach - Google Patents
Reactive compound and its preparation method and application in Shenzhou City's honey peach Download PDFInfo
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- 235000006040 Prunus persica var persica Nutrition 0.000 title claims abstract description 44
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- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 240000006413 Prunus persica var. persica Species 0.000 title 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
- C07H15/02—Acyclic radicals, not substituted by cyclic structures
- C07H15/04—Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of the saccharide radical
- C07H15/10—Acyclic radicals, not substituted by cyclic structures attached to an oxygen atom of the saccharide radical containing unsaturated carbon-to-carbon bonds
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The present invention provides reactive compound and its preparation method and application in Shenzhou City's honey peach.After Shenzhou City's honey peach fruit is crushed first, it is placed in methanol and extracts, filtering and concentrating, and extract isolate and purify and obtains two sequiterpene glycoside reactive compounds, one of them is novel compound, is named as SZMT01.Two compounds that the present invention is extracted from Shenzhou City's honey peach can significantly extend the replicability service life of yeast cells, have anti-senescence function.It finds that SZMT01 is the expression by adjusting sod gene by the analysis to SZMT01 anti-aging mechanism, improves the oxidation resistance of yeast, to achieve the purpose that extend the yeast cells replicability service life.The survival rate of yeast can be improved under the conditions of oxidative stress description of test SZMT01 is existing for the hydrogen peroxide.The present invention being studied in terms of delaying senescence and treating senile disease for Shenzhou City's honey peach reactive compound, has important practical significance to new drug development.
Description
Technical field
The present invention relates to two kinds of reactive compounds and preparation method thereof in pharmaceutical technology field more particularly to Shenzhou City's honey peach
And application.
Background technique
According to recent statistics data, the whole world 60 years old and the above aged had reached 9.01 hundred million in 2014, in advance
Counting to this number of the year two thousand fifty will be more than 2,000,000,000.The world has entered aging society, the following disease relevant to aging
Disease has become increasingly significant problem, and the neurodegenerative disease including Alzheimer disease, Parkinson is global
One of difficult medical problem.Only in China, the patient populations of Alzheimer disease had been increased to 5,690,000 in 2010.It is led in medicine and pharmacology
Domain, the drug for finding prevention and treatment geriatric disease have become the task of top priority.
By studying for a long period of time to Aging mechanism, a variety of causes of senescence have been formed in the art, including programmed aging is said, body
Cell mutation is said, mistake is caused disaster, and free radical is said, neuroendocrine is said, immunosenescence is said etc..It can be seen that aging is one
A sufficiently complex process, even same class antiaging agent, play drug effect mode be also not quite similar, each other without
Positive connection.
Shenzhou City's honey peach derives from Shenzhou City, Hebei province, and this place has unique geographical environment and edaphic condition, abounds with honey peach,
Bright characteristic.Shenzhou City is also named as " township of Chinese honey peach " by the State Administration of Forestry.Shenzhou City's honey peach head is very large, fruit type is unique,
Color is gorgeous, fruit top spur, suture is deep, thin skin meat is thin, juice sweet tea such as honey, the unique special favor of aromatic flavour, is known as
King in peach, also known as stalwart peach.After more than 2,000 years, always as royal tribute, it is exclusive rare kind, is called " tribute peach " in history.
Summary of the invention
The object of the present invention is to provide the reactive compound 1 (compound SZMT01) and compound 2 in a kind of Shenzhou City's honey peach,
Reactive compound in Shenzhou City's honey peach is sequiterpene glycosides compound, and wherein compound 1 is that Sesquiterpene Polyesterss glycoside is newly tied
Structure compound, two kinds of reactive compounds have stronger anti-aging ability.Its structural formula is as follows:
The physicochemical property of above-mentioned Shenzhou City's honey peach reactive compound 1 are as follows: colourless powder；(c 0.16,MeOH)；
Molecular formula is C21H34O8；High resolution mass spec ESI-TOF-MS m/z 437.2151 [M+Na]+, theoretical value:: C21H34O8Na+
437.2151；1H and13C NMR is as shown in table 1.
The physicochemical property of reactive compound 2 are as follows: colourless powder；(c 0.2,MeOH)；Molecular formula is
C21H36O7；High resolution mass spec ESI-TOF-MS m/z 423.2368 [M+Na]+, theoretical value: C21H36O7Na+423.2359；13C
NMR(125MHz,CD3CD) data are as follows: δC 14.1(C-13),16.0(C-14),23.7(C-8),27.4(C-4),27.6(C-
15),40.3(C-5),43.4(C-9),62.8(C-6′),71.8(C-4′),73.9(C-10),75.1(C-2′),76.0(C-
1),77.9(C-5′),78.2(C-3′),102.6(C-1′),112.0(C-12),126.0(C-7),129.9(C-3),132.9
(C-2),135.7(C-6),146.4(C-11)。
It is a further object to provide two reactive compounds 1 (compound SZMT01) and chemical combination in Shenzhou City's honey peach
The preparation method of object 2, is realized by following steps:
(1) Shenzhou City's honey peach fruit is taken, after crushing, is placed in methanol and extracts, filtering and concentrating obtains extract；
(2) extract is isolated and purified, obtains Shenzhou City's honey peach reactive compound 1 and compound 2.
Preferably, in step (1), the temperature of the extraction is 25~30 DEG C, the time is 0.5~for 24 hours.It is further preferred that institute
The temperature for stating extraction is 25 DEG C of room temperatures, and the time is for 24 hours.
Further, in step (2), the process isolated and purified includes:
(a) with methanol: aqueous solvent system makees eluant, eluent, carries out first time separation to extract, obtains target fraction I；
(b) with methanol: aqueous solvent system makees eluant, eluent, carries out second to the target fraction I and separates, and obtains activity and evaporates
Divide II and active fractions III；
(c) using methanol aqueous solution as mobile phase, third is carried out to active fractions II and active fractions III using reversed-phase HPLC
Secondary separation obtains Shenzhou City's honey peach reactive compound 1 and compound 2；
Wherein: in step (a), being separated using octadecyl silane opening column；Methanol: aqueous solvent system presses body
Product is successively eluted than 30:70,50:50,70:30,100:0, and the fraction of volume ratio 70:30 elution is target fraction I.
In step (b), separated using octadecyl silane opening column；Methanol: aqueous solvent system is according to volume
It is successively eluted than 50:50,55:45,60:40,65:35,70:30,80:20,100:0, volume ratio 60:40 and 65:35 elution
Fraction is active fractions II and active fractions III.
In step (c), the mobile phase is 63% methanol aqueous solution；In separation process, taking retention time is 13.9 minutes
Fraction, obtain Shenzhou City's honey peach reactive compound 1.
In step (c), the mobile phase is 65% methanol aqueous solution；In separation process, taking retention time is 14.5 minutes
Fraction, obtain Shenzhou City's honey peach reactive compound 2.
It is also another object of the present invention to provide Shenzhou City's honey peach reactive compounds to prepare antiaging agent or health care
Application in product, such as anti-aging honey peach drink etc. is made in Shenzhou City's honey peach.By Shenzhou City's honey peach reactive compound and pharmaceutically may be used
The carrier of receiving is made.Studies have shown that compound SZMT01 and compound 2 be in the in-vitro screening model of anti-senescence compounds,
It can significantly extend the replicability service life of yeast cells.
The pharmaceutically acceptable carrier refers to the pharmaceutical carrier of pharmaceutical field routine, such as filler, adhesive, moistens
Agent, sorbefacient, surfactant etc..
Starch, sucrose or microcrystalline cellulose can be used in the filler；Starch slurry, hydroxypropyl fiber can be used in described adhesive
Element, gelatin or polyethylene glycol；Magnesium stearate, superfine silica gel powder or polyethylene glycols can be used in the wetting agent；The absorption enhancement
Poly- sorb rouge or lecithin can be used in agent；Poloxamer, fatty acid sorbitan or poly- sorb rouge can be used in the surfactant.
In addition it can which other adjuvants such as flavouring agent, sweetener etc. are added.
The dosage form of the antiaging agent or health care product can be tablet, pill, pulvis, dispersible tablet, sachets, the wine made of broomcorn millet
Agent, suspension, emulsion, solution, syrup, aerosol, soft capsule, hard capsule, aseptic parenteral solution, liniment or suppository；It can make
At routine, quick-release, sustained release or delayed release preparation.
Antiaging agent of the invention can be given by all means, including oral, nasal cavity, intramuscular injection, subcutaneous injection,
Intravenous injection etc..
In order to further probe into the mechanism of action of the compound SZMT01 in terms of anti-aging, we are tested by oxidative stress
Anti-aging Preliminary Study on Mechanism is carried out to compound SZMT01, under the conditions of finding compound SZMT01 existing for the hydrogen peroxide
The survival rate of yeast can be improved, while it has also been found that the compound is to improve the antioxygen of yeast by the expression of adjusting sod gene
Change ability, to realize yeast cells replicability life-time dilatation.
According to oxyradical theory, oxidative pressure is the main reason for leading to aging.Even if line grain under normal operation
Body can also generate harmful metabolite-active oxygen (ROS), these substances can destroy cell membrane and large biological molecule, such as egg
White and nucleic acid, and then the function of cell is destroyed, cause aging and death.
SOD is a gene relevant to anti-oxidation stress.Compound SZMT01 cannot extend the yeast after SOD is knocked out
The replicability service life, and then prove that SZMT01 is the expression by adjusting sod gene, to realize the yeast cells replicability service life
It is extended.
Compared with prior art, the invention has the following advantages:
(1) in yeast aging model K6001 brewing yeast cell, compound that the present invention is extracted from Shenzhou City's honey peach
SZMT01 and 2 can significantly extend the replicability service life of yeast cells, have very strong anti-aging ability.
(2) honey peach reactive compound in Shenzhou City's of the present invention to delay senescence and treatment senile disease in terms of new drug development into
Row basic research, has important practical significance.
Detailed description of the invention
Fig. 1 is Shenzhou City's honey peach reactive compound 1 (A) prepared in embodiment 2 and compound 2 (B) to yeast K6001
The influence result in replicability service life；Wherein, Control indicates negative control；Res (Resveratrol) is the white Chenopodiaceae of positive control
Reed alcohol (10 μM)；1 7.5 μM of 1,1 25 μM of compound compounds 1 for indicating that concentration is 25 μM for indicating that concentration is 7.5 μM；2
7.5 μM of 2,2 25 μM of compound compounds 2 for indicating that concentration is 25 μM for indicating that concentration is 7.5 μM.
Fig. 2 is reactive compound 1 in embodiment 3 to mutant yeast strains Δ sod1 (A) and Δ sod2 (B) replicability service life
Influence result；Wherein, Control (K6001) indicates the K6001 yeast for being not added with reactive compound 1；Control(Δ
Sod1) and Control (Δ sod2) respectively indicates the K6001 mutant yeast strains Δ sod1 and Δ for being not added with reactive compound 1
sod2；1 7.5 μM (Δ sod1) and 1 7.5 μM (Δ sod2) respectively indicate the K6001 yeast for adding 7.5 μM of reactive compounds 1
Mutant strain Δ sod1 and Δ sod2.
Fig. 3 is 1 oxidative stress experimental result of reactive compound in embodiment 3；Wherein, Control (K6001) expression does not add
Add the K6001 yeast of reactive compound 1；Control indicates negative control；Res (Resveratrol) is the white black false hellebore of positive control
Alcohol (10 μM)；1 7.5 μM of 1,1 25 μM of compound compounds 1 for indicating that concentration is 25 μM for indicating that concentration is 7.5 μM.
Specific embodiment
The present invention is described further in conjunction with the accompanying drawings and embodiments.
Embodiment 1
1, in Shenzhou City's honey peach reactive compound 1 and compound 2 preparation, the specific steps are as follows:
(1) Shenzhou City 3.0kg honey peach is placed in methanol (technical grade), extracts 24 hours (concussion) at room temperature；It is dense through filtering
After contracting, methanol extract 100.0g is obtained.
(2) methanol extract is separated with octadecyl silane opening column, with methanol: aqueous solvent system is washed
De- agent, by methanol: volume ratio=30:70,50:50,70:30,100:0 of water is successively eluted, and volume ratio 70:30 is taken to elute
Fraction 0.7g.
(3) step (2) taken fraction is purified with octadecyl silane opening column, with methanol: aqueous solvent system
System makees eluant, eluent, and by methanol: volume ratio=50:50 of water, 55:45,60:40,65:35,70:30,80:20,100:0 are successively washed
It is de-, the fraction 3.7mg and 2.3mg for taking volume ratio 60:40 and 65:35 to elute respectively.
(4) step (3) taken fraction is taken, is purified with reverse hplc, chromatographic condition: permaphase ODS-HG-5 (10/
250mm), flow velocity 3ml/min, Detection wavelength 210nm, mobile phase are 63% methanol aqueous solution, obtain 1 1.1mg of reactive compound
(retention time 13.9min)；Mobile phase is 65% methanol aqueous solution, and obtaining 2 1.8mg of reactive compound, (retention time is
14.5min).2, the physicochemical characteristics and chemical structure analysis of reactive compound 1 and compound 2
Through13C NMR、1H NMR, HRMS, HSQC, HMBC, NOESY analysis, as a result as follows:
The physicochemical property of compound 1: colourless powder, molecular formula C21H34O8, high resolution mass spec ESI-TOF-MS m/
z437.2151[M+Na]+。1H NMR and13The data of C NMR see the table below 1.
Table 111H NMR and13C NMR data (CD3OD；δ/ppm,J/Hz).
a500MHz,b125MHz
The physicochemical property of compound 2: colourless powder, molecular formula C21H36O7；High resolution mass spec ESI-TOF-MS m/
z423.2368[M+Na]+。13C NMR(125MHz,CD3CD) data are as follows: δC 14.1(C-13),16.0(C-14),23.7(C-
8),27.4(C-4),27.6(C-15),40.3(C-5),43.4(C-9),62.8(C-6′),71.8(C-4′),73.9(C-10),
75.1(C-2′),76.0(C-1),77.9(C-5′),78.2(C-3′),102.6(C-1′),112.0(C-12),126.0(C-
7),129.9(C-3),132.9(C-2),135.7(C-6),146.4(C-11)。
The structural formula of compound 1 and compound 2 is as follows；
The activity of fighting against senium of reactive compound 1 (SZMT01) and compound 2 is analyzed in 2 Shenzhou City's honey peach of embodiment
Currently, the biological model for research on anti-senescence mainly has mouse, nematode, drosophila and yeast.The present embodiment selection
Active system of the saccharomyces cerevisiae as research on anti-senescence；Because yeast is single celled eucaryote, life cycle is short, has obtained it
Complete genomic data is currently used aging model biology.
At the same time, using resveratrol as positive control, resveratrol be it is currently known, in many animals mould
The small molecule compound of anti-aging effects is shown in type.
The step of analysis method, is as follows:
(1) K6001 yeast strain is taken out from -30 DEG C of refrigerators, is washed three times with PBS, each 5ml removes glycerol therein；
Add 1ml PBS, blow and beat, make its suspend after be added to 5ml fluid nutrient medium (1% yeast powder, 2% peptone, 3%
Galactolipin) in；28 DEG C of shakings (160r/min) are cultivated 24 hours.
(2) it after cultivating, is washed three times with 5ml PBS, removes fluid nutrient medium therein, with blood counting chamber meter
Number, calculates the concentration of yeast.
(3) solvent is made using dehydrated alcohol, prepares 7.5 μM, the 1 of 25 μM and 2,10 μM of resveratrol is spare.
(4) be added in the culture dish of sterilizing 5ml solid medium (1% yeast powder, 2% peptone, 2%
Glucose, 2% agar powder), after culture medium solidification, it is separately added into prepared sample in step (3) thereto, solvent is waved
After hair be added 4000 yeast, with spreader smear uniformly, 28 DEG C constant temperature incubation 48 hours.
(5) every ware counts the daughter cell number that 40 mother cells generate respectively at random under microscope, and records, maps and divide
Analysis, the result is shown in Figure 1.
As shown in Figure 1, the average life span of negative control (being not added with sample, Control) is 7.10 ± 0.34, positive control
(resveratrol of 10 μM of addition, Resverstrol) is 8.40 ± 0.47*；7.5 μM, the 1 of 25 μM of concentration is 8.87 respectively ±
0.53**,8.37±0.51*；7.5 μM, the 2 of 25 μM of concentration are 8.40 ± 0.54*, 8.20 ± 0.41* respectively.(*p<0.05,**
p<0.01).
Therefore, compound 1 and compound 2 can extend the replicability service life of yeast.
1 mechanism of resisting senility of reactive compound is analyzed in 3 Shenzhou City's honey peach of embodiment
1, whether test 1 is able to extend the K6001 yeast mutation bacterium for having knocked out sod gene under 7.5 μM of active concentration
The replicability service life of strain Δ sod1 and Δ sod2.
The step of analysis method, is as follows:
(1) K6001 yeast strain and K6001 mutant yeast strains Δ sod1 and Δ sod2 are taken out from -30 DEG C of refrigerators, used
PBS is washed three times, and each 5ml removes glycerol therein；1ml PBS is added, is blown and beaten, is added to 5ml liquid after so that it is suspended
In culture medium (1% yeast powder, 2% peptone, 3% galactolipin)；28 DEG C of shakings (160r/min) are cultivated 24 hours.
(2) it after cultivating, is washed three times with 5ml PBS, removes fluid nutrient medium therein, with blood counting chamber meter
Number, calculates the concentration of yeast.
(3) solvent is made using dehydrated alcohol, prepares the 1 of 7.5 μM, it is spare.
(4) be added in the culture dish of sterilizing 5ml solid medium (1% yeast powder, 2% peptone, 2%
Glucose, 2% agar powder), after culture medium solidification, it is separately added into prepared sample in step (3) thereto, solvent is waved
After hair.4000 yeast are added, and (K6001 yeast strain or K6001 mutant yeast strains Δ sod1 and Δ sod2, use spreader
Smear uniformly, 28 DEG C constant temperature incubation 48 hours.
(5) every ware counts the daughter cell number that 40 mother cells generate respectively at random under microscope, and records, maps and divide
Analysis, is as a result shown in Fig. 2.
As shown in Figure 2, on K6001 bacterial strain negative control (being not added with sample, Control) average life span be 6.85 ±
0.42, positive control (resveratrol of 10 μM of addition, Resverstrol) is 8.70 ± 0.48**, and the 1 of 7.5 μM of concentration is
8.40±0.48*；The average life span of negative control (being not added with sample, Control) is on K6001 mutant yeast strains Δ sod1
6.55 ± 0.33, positive control (resveratrol of 10 μM of addition, Resverstrol) is the 1 of 6.95 ± 0.32,7.5 μM of concentration
It is 7.05 ± 0.37；The average life span of negative control (being not added with sample, Control) on K6001 mutant yeast strains Δ sod2
It is 6.65 ± 0.36, positive control (resveratrol of 10 μM of addition, Resverstrol) is 7.17 ± 0.46,7.5 μM of concentration
1 is 7.25 ± 0.32.(*p<0.05,**p<0.01)
Therefore, compound 1 can not extend the K6001 yeast mutation for having knocked out sod gene under 7.5 μM of active concentration
The replicability service life of bacterial strain Δ sod1 and Δ sod2.
The activity analysis of embodiment 5SZMT01 raising yeast survival rate
Can test SZMT01 improve the survival rate of yeast, and test method is as follows:
(1) the wild-type yeast BY4741 that -30 DEG C save is washed three times with 5ml PBS, removes glycerol therein；It is added
1ml sterile water, piping and druming make its suspension, are added to 5ml dextrose culture-medium (1% yeast powder, 2% peptone, 2% Portugal
Grape sugar) in；Shaking table is put it into, 28 DEG C of shakings (160r/min) are cultivated 24 hours, its restoration ecosystem ability is made.
(2) BY4741 is inoculated into the new dextrose culture-medium of 25mL, adjusts OD600Value be 0.1, then respectively with 7.5 μ
M, 25 μM of cholesterol and positive reference substance (10 μM of resveratrol, Resverstrol) are incubated for 12 hours.
(3) the 5 μ L drop of culture solution for taking each group to contain identical yeast cells is containing 9mM H2O2Dextrose solid medium
On (1% yeast powder, 2% peptone, 2% glucose, 2% agar)；28 DEG C culture 3 days after observe yeast growth shape
Condition is simultaneously taken a picture, and observation result is shown in Fig. 3.
As seen from Figure 3, compared with negative control, containing 9mM H2O2Dextrose solid medium on, 7.5 μM, 25 μM
SZMT01 significantly improve the survival rate of yeast.
Claims (4)
1. reactive compound in a kind of Shenzhou City's honey peach, which is characterized in that Shenzhou City's honey peach reactive compound is known as compound 1,
Its structural formula are as follows:
2. the preparation method of reactive compound in a kind of Shenzhou City's honey peach, which is characterized in that the compound is respectively 1 He of compound
Compound 2, is realized by following steps:
(1) Shenzhou City's honey peach fruit is taken, after crushing, is placed in methanol and shakes extraction, filtering and concentrating obtains extract；The extraction
Temperature be room temperature, the time be for 24 hours；
(2) extract is isolated and purified, obtains Shenzhou City's honey peach reactive compound；
The structural formula of compound 1 and compound 2 is as follows:
Described isolate and purify is realized by following steps:
(a) with methanol: aqueous solvent system makees eluant, eluent, carries out first time separation to extract, obtains target fraction I, methanol: water
30:70,50:50,70:30,100:0 are successively eluted solvent system by volume, and the fraction of volume ratio 70:30 elution evaporates for target
Divide I；
(b) with methanol: aqueous solvent system makees eluant, eluent, carries out second to the target fraction I and separates, obtains active fractions II
And III, methanol: aqueous solvent system is according to volume ratio 50:50, and 55:45,60:40,65:35,70:30,80:20,100:0 are successively
Elution, the fraction of volume ratio 60:40 and 65:35 elution are active fractions II and active fractions III；
(c) using methanol aqueous solution as mobile phase, third time separation is carried out to active fractions II and III using reversed-phase HPLC, respectively
Shenzhou City's honey peach reactive compound 1 and compound 2 are obtained, wherein mobile phase is 63% methanol aqueous solution；In separation process, take
The fraction that retention time is 13.9 minutes, obtains Shenzhou City's honey peach reactive compound 1, and mobile phase is 65% methanol aqueous solution；Separation
In the process, taking retention time is 14.5 minutes fractions, obtains Shenzhou City's honey peach reactive compound 2.
3. Shenzhou City's honey peach reactive compound that method prepares according to claim 2 is preparing antiaging agent or health care
Application in product, the compound are compound 1 and compound 2.
4. application according to claim 3, which is characterized in that the drug or health care product are by depth described in claim 1
Two reactive compounds of state honey peach and pharmaceutically acceptable carrier are made.
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| Glucosylated sesquiterpene alcohols from Fraxinus Glucosylated sesquiterpene alcohols from Fraxinus;Macahig, Rene Angelo等;《Natural Product Communications》;20121231;第7卷(第4期);第467-470页 |
| Thiophenes, polyacetylenes and terpenes from the aerial parts of Eclipta prostrata;Xi, Feng-Min等;《Bioorganic & Medicinal Chemistry》;20141231;第22卷(第22期);参见第6515-6522页，特别是摘要、图1、表6、3.3提取和分离 |
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Effective date of registration: 20231023 Address after: No.98 Baoheng Road, Shenzhou City, Hengshui City, Hebei Province, 052800 Patentee after: Hebei Kaixiang Health Technology Co.,Ltd. Address before: 310058 Yuhang Tang Road, Xihu District, Hangzhou, Zhejiang 866 Patentee before: ZHEJIANG University |