CN106962815A - Low fat pork balls added with honeycomb polysaccharide and preparation method thereof - Google Patents
Low fat pork balls added with honeycomb polysaccharide and preparation method thereof Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
- A23B4/00—Preservation of meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Meat, Egg Or Seafood Products (AREA)
- Cosmetics (AREA)
Abstract
本发明提供一种添加有巢脾多糖的低脂猪肉丸及其制作方法。所述猪肉丸包括如下重量份数的原料:瘦猪肉1000份、巢脾多糖6份、切碎脂肪40份,玉米淀粉100份、料酒20份、冰水150份、盐32份、白胡椒粉2份、小葱2份、蒜2份、生姜5份、味精0.05份。所述制作方法包括:从蜜蜂巢脾中提取巢脾多糖;将瘦猪肉清洗、去皮、剔除可见脂肪和结缔组织,切块备用;将脂肪切碎;向1000g瘦猪肉中添加6g巢脾多糖,斩拌5分钟;加入40g切碎脂肪、100g淀粉、20g料酒、150g冰水、32g盐、2g白胡椒粉、2g小葱、2g蒜、5g生姜、0.05g味精,斩拌混合5分钟;4℃下腌制12小时;取出搓成肉丸,在开水中煮制5‑8分钟,捞出冷却。本发明能够在降低猪肉丸中脂肪含量的同时保证猪肉丸的品质,并可延长其储存期。
The invention provides a low-fat pork ball added with honeycomb polysaccharide and a preparation method thereof. The pork balls include the following raw materials in parts by weight: 1000 parts of lean pork, 6 parts of honeycomb polysaccharide, 40 parts of chopped fat, 100 parts of cornstarch, 20 parts of cooking wine, 150 parts of ice water, 32 parts of salt, white pepper powder 2 parts, 2 parts shallot, 2 parts garlic, 5 parts ginger, 0.05 parts monosodium glutamate. The preparation method comprises: extracting honeycomb polysaccharide from honeycomb honeycomb; washing and peeling the lean pork, removing visible fat and connective tissue, and cutting into pieces for later use; chopping the fat; adding 6 g of honeycomb polysaccharide to 1000 g of lean pork , chop and mix for 5 minutes; add 40g chopped fat, 100g starch, 20g cooking wine, 150g ice water, 32g salt, 2g white pepper, 2g shallots, 2g garlic, 5g ginger, 0.05g monosodium glutamate, chop and mix for 5 minutes; 4 Marinate at ℃ for 12 hours; take out and knead into meatballs, cook in boiling water for 5-8 minutes, remove and cool. The invention can ensure the quality of the pork meatballs while reducing the fat content in the pork meatballs, and can prolong the storage period of the pork meatballs.
Description
技术领域technical field
本发明涉及肉制品技术领域,尤其涉及一种添加有巢脾多糖的低脂猪肉丸及其制作方法。The invention relates to the technical field of meat products, in particular to a low-fat pork ball added with honeycomb polysaccharide and a preparation method thereof.
背景技术Background technique
肉及其肉制品因其味道鲜美、营养丰富而深受消费者的喜爱。在肉制品中,脂肪的使用有利于提高风味、改善质构。因此目前市场上提供的大多数肉制品,脂肪含量都较高。但是,人们过度摄入高脂肪的产品会引发高血压、高血脂、冠心病等一系列慢性疾病。因此,如何既能降低脂肪含量,又能保证肉制品的品质是肉制品行业发展亟待解决的问题。Meat and its meat products are deeply loved by consumers because of their delicious taste and rich nutrition. In meat products, the use of fat is beneficial to enhance flavor and improve texture. Therefore, most of the meat products currently on the market are high in fat. However, people's excessive intake of high-fat products can cause a series of chronic diseases such as hypertension, hyperlipidemia, and coronary heart disease. Therefore, how to reduce the fat content and ensure the quality of meat products is an urgent problem to be solved in the development of the meat products industry.
发明内容Contents of the invention
本发明提供的添加有巢脾多糖的低脂猪肉丸及其制作方法,能够在有效降低脂肪含量的同时保证猪肉丸的品质,同时还能延长其储藏期。The low-fat pork balls added with honeycomb polysaccharide and the preparation method thereof provided by the invention can effectively reduce the fat content, ensure the quality of the pork balls, and prolong the storage period of the pork balls.
第一方面,本发明提供一种添加有巢脾多糖的低脂猪肉丸,包括如下重量份数的原料:瘦猪肉1000份、巢脾多糖6份、切碎脂肪40份,玉米淀粉100份、料酒20份、冰水150份、盐32份、白胡椒粉2份、小葱2份、蒜2份、生姜5份、味精0.05份。In the first aspect, the present invention provides a low-fat pork ball added with honeycomb polysaccharide, comprising the following raw materials in parts by weight: 1000 parts of lean pork, 6 parts of honeysuckle polysaccharide, 40 parts of chopped fat, 100 parts of cornstarch, 20 parts of cooking wine, 150 parts of ice water, 32 parts of salt, 2 parts of white pepper, 2 parts of shallots, 2 parts of garlic, 5 parts of ginger, and 0.05 parts of monosodium glutamate.
可选地,所述瘦猪肉为猪后腿肉。Optionally, the lean pork is pork ham.
可选地,所述巢脾多糖为从使用三年以上的蜜蜂老巢脾中提取出来。Optionally, the honeycomb polysaccharide is extracted from old honeybee honeycombs that have been used for more than three years.
第二方面,本发明提供一种添加有巢脾多糖的低脂猪肉丸的制作方法,包括:In a second aspect, the present invention provides a method for making low-fat pork balls added with honeycomb polysaccharide, comprising:
从蜜蜂巢脾中提取巢脾多糖;Extract honeycomb polysaccharide from honeycomb;
将瘦猪肉清洗、去皮、剔除可见脂肪和结缔组织,切块备用;Wash the lean pork, peel it, remove visible fat and connective tissue, and cut it into pieces for later use;
将脂肪切碎;chop the fat;
向1000g瘦猪肉中添加6g巢脾多糖,斩拌5分钟;Add 6g honeycomb polysaccharide to 1000g lean pork, chop and mix for 5 minutes;
加入40g切碎脂肪、100g淀粉、20g料酒、150g冰水、32g盐、2g白胡椒粉、2g小葱、2g蒜、5g生姜、O.05g味精,斩拌混合5分钟;Add 40g chopped fat, 100g starch, 20g cooking wine, 150g ice water, 32g salt, 2g white pepper, 2g shallots, 2g garlic, 5g ginger, 0.05g monosodium glutamate, chop and mix for 5 minutes;
4℃下腌制12小时;Marinate at 4°C for 12 hours;
取出搓成肉丸,在烧开的水中煮制5-8分钟,捞出冷却。Take out and knead into meatballs, cook in boiling water for 5-8 minutes, remove and cool.
可选地,所述从蜜蜂巢脾中提取巢脾多糖包括:Optionally, said extracting honeycomb polysaccharide from honeycomb honeycomb includes:
将蜜蜂巢脾粉碎;crush the honeycomb;
使用40目筛网进行过筛;Use 40 mesh sieves to sieve;
放入水中煮沸1小时;Boil in water for 1 hour;
使用120目纱布进行过滤,重复4次;Use 120 mesh gauze to filter, repeat 4 times;
合并滤液;combined filtrate;
加热浓缩;heat concentration;
离心10分钟;Centrifuge for 10 minutes;
取上清液;Take the supernatant;
加入浓度为80%的乙醇进行沉淀;Adding a concentration of 80% ethanol for precipitation;
离心;Centrifugal;
烘干,得到巢脾多糖。drying to obtain honeycomb polysaccharide.
可选地,所述瘦猪肉为猪后腿肉。Optionally, the lean pork is pork ham.
可选地,所述蜜蜂巢脾为使用三年以上的蜜蜂老巢脾。Optionally, the honeycomb is an old honeycomb that has been used for more than three years.
本发明实施例提供的添加有巢脾多糖的低脂猪肉丸及其制作方法,使用蜜蜂巢脾多糖替代猪肉丸中的部分脂肪,能够有效降低脂肪含量,同时改善低脂猪肉丸的色泽、弹性、硬度、咀嚼性,保证猪肉丸的品质,还可以延长猪肉丸的储藏期。此外,蜜蜂巢脾中含有丰富的生物活性成分如生物碱、有机酸、树脂、酶类、氨基酸、蛋白质、多糖以及昆虫激素等成分,从蜜蜂巢脾中提取的巢脾多糖是具有抗氧化、增强免疫力等生物学活性的高分子物质,从而使得添加有巢脾多糖的猪肉丸有益于人体健康。The low-fat pork balls added with honeycomb polysaccharide and the production method thereof provided by the embodiments of the present invention use honeycomb polysaccharide to replace part of the fat in the pork balls, which can effectively reduce the fat content and improve the color and elasticity of the low-fat pork balls at the same time , hardness, chewiness, ensure the quality of pork balls, and can also extend the storage period of pork balls. In addition, honeycomb contains rich biologically active components such as alkaloids, organic acids, resins, enzymes, amino acids, proteins, polysaccharides and insect hormones. Honeycomb polysaccharides extracted from honeycomb are antioxidant, The high-molecular substances that enhance immunity and other biological activities make the pork balls added with honeycomb polysaccharide beneficial to human health.
附图说明Description of drawings
图1为本发明实施例提供的添加有巢脾多糖的低脂猪肉丸的制作方法的流程图;Fig. 1 is the flow chart of the preparation method of the low-fat pork ball that is added with honeycomb polysaccharide provided by the embodiment of the present invention;
图2为本发明实施例提供巢脾多糖的脂肪替代量对产品的色差影响示意图;Fig. 2 is a schematic diagram of the effect of the fat replacement amount of honeycomb polysaccharide on the color difference of the product provided by the embodiment of the present invention;
图3为本发明实施例提供巢脾多糖的脂肪替代量对产品的保水性影响示意图;Fig. 3 is a schematic diagram of the effect of the fat replacement amount of honeycomb polysaccharide on the water retention of the product provided by the embodiment of the present invention;
图4a为本发明实施例提供巢脾多糖的脂肪替代量对产品弹性影响示意图;Figure 4a is a schematic diagram of the effect of the fat replacement amount of honeycomb polysaccharide on product elasticity provided by the embodiment of the present invention;
图4b为本发明实施例提供巢脾多糖的脂肪替代量对产品硬度影响示意图;Fig. 4b is a schematic diagram of the effect of the amount of fat replacement of honeycomb polysaccharide on the hardness of the product provided by the embodiment of the present invention;
图4c为本发明实施例提供巢脾多糖的脂肪替代量对产品咀嚼性影响示意图;Figure 4c is a schematic diagram of the influence of the amount of fat replacement of honeycomb polysaccharide on the chewiness of the product provided by the embodiment of the present invention;
图5a为本发明实施例提供巢脾多糖猪肉丸不同储存期酸价测定结果的示意图;Figure 5a is a schematic diagram of the acid value measurement results of different storage periods provided by the embodiment of the present invention;
图5b为本发明实施例提供的巢脾多糖猪肉丸不同储存期挥发性盐基氮的测定结果的示意图。Fig. 5b is a schematic diagram of the measurement results of volatile base nitrogen in different storage periods of the spleen polysaccharide pork balls provided by the embodiment of the present invention.
具体实施方式detailed description
为使本发明实施例的目的、技术方案和优点更加清楚,下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is only some embodiments of the present invention, but not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.
本发明实施例提供一种添加有巢脾多糖的低脂猪肉丸,包括如下重量份数的原料:瘦猪肉1000份、巢脾多糖6份、切碎脂肪40份,玉米淀粉100份、料酒20份、冰水150份、盐32份、白胡椒粉2份、小葱2份、蒜2份、生姜5份、味精0.05份。An embodiment of the present invention provides a low-fat pork ball added with honeycomb polysaccharide, which includes the following raw materials in parts by weight: 1000 parts of lean pork, 6 parts of honeysuckle polysaccharide, 40 parts of chopped fat, 100 parts of cornstarch, and 20 parts of cooking wine 150 parts of ice water, 32 parts of salt, 2 parts of white pepper, 2 parts of shallots, 2 parts of garlic, 5 parts of ginger, and 0.05 parts of monosodium glutamate.
可选地,所述瘦猪肉为猪后腿肉。Optionally, the lean pork is pork ham.
可选地,所述巢脾多糖为从使用三年以上的蜜蜂老巢脾中提取出来。Optionally, the honeycomb polysaccharide is extracted from old honeybee honeycombs that have been used for more than three years.
本发明实施例还提供一种添加有巢脾多糖的低脂猪肉丸的制作方法,如图1所示,所述方法包括:The embodiment of the present invention also provides a method for making low-fat pork balls added with honeycomb polysaccharide, as shown in Figure 1, the method comprising:
S11、从蜜蜂巢脾中提取巢脾多糖。S11. Extracting honeycomb polysaccharide from honeycomb honeycomb.
可选地,所述蜜蜂巢脾为使用三年以上的蜜蜂老巢脾。Optionally, the honeycomb is an old honeycomb that has been used for more than three years.
具体地,可以采用如下流程从蜜蜂巢脾中提取巢脾多糖:Specifically, the following process can be used to extract honeycomb polysaccharides from honeybee honeycomb:
(1)将蜜蜂巢脾粉碎;(1) the honeycomb is crushed;
(2)使用40目筛网进行过筛;(2) use 40 mesh sieves to sieve;
(3)放入水中煮沸1小时;(3) put into water and boil for 1 hour;
(4)使用120目纱布进行过滤,重复4次;(4) Use 120 mesh gauze to filter, repeat 4 times;
(5)合并滤液;(5) merge filtrate;
(6)加热浓缩;(6) heating and concentrating;
(7)离心10分钟;(7) centrifuge for 10 minutes;
(8)取上清液;(8) get the supernatant;
(9)加入浓度为80%的乙醇进行沉淀;(9) adding concentration is 80% ethanol to carry out precipitation;
(10)离心;(10) centrifugal;
(11)烘干,得到巢脾多糖。(11) drying to obtain honeycomb polysaccharide.
S12、将瘦猪肉清洗、去皮、剔除可见脂肪和结缔组织,切成肉块。S12. Wash the lean pork, peel it, remove visible fat and connective tissue, and cut it into pieces.
可选地,所述瘦猪肉为猪后腿肉。Optionally, the lean pork is pork ham.
S13、将脂肪切碎。S13. Mince the fat.
S14、向1000g瘦猪肉中添加6g巢脾多糖,斩拌5分钟。S14. Add 6 g of nest spleen polysaccharide to 1000 g of lean pork, chop and mix for 5 minutes.
S15、加入40g切碎脂肪、100g淀粉、20g料酒、150g冰水、32g盐、2g白胡椒粉、2g小葱、2g蒜、5g生姜、0.05g味精,斩拌混合5分钟。S15. Add 40g chopped fat, 100g starch, 20g cooking wine, 150g ice water, 32g salt, 2g white pepper, 2g shallots, 2g garlic, 5g ginger, 0.05g monosodium glutamate, chop and mix for 5 minutes.
S16、4℃下腌制12小时。Marinate for 12 hours at S16, 4°C.
S17、取出搓成肉丸,在烧开的水中煮制5-8分钟,捞出冷却。S17. Take out and knead into meatballs, cook in boiling water for 5-8 minutes, remove and cool.
最后,将冷却后的猪肉丸用保鲜袋分装后,于4℃的冰箱里储存待测。Finally, after the cooled pork balls were packed in fresh-keeping bags, they were stored in a refrigerator at 4°C for testing.
下面从保水性、色泽、质构检测(弹性、硬度、咀嚼性)、感官评定、保鲜性能等方面对采用上述制作方法制作出的添加有巢脾多糖的低脂猪肉丸进行检测。The low-fat pork balls added with honeycomb polysaccharide produced by the above production method are tested from the aspects of water retention, color, texture detection (elasticity, hardness, chewiness), sensory evaluation, and fresh-keeping performance.
具体检测过程如下:The specific detection process is as follows:
(1)保水性测定(1) Determination of water retention
参照Perez-mate′s等方法,并稍作改动。称取3g研碎的猪肉丸样品,用滤纸包住,放置50ml离心管(底部放一小块棉花球),在25℃下以6000r/min转速离心10min,然后称其前后质量。Refer to methods such as Perez-mate's with slight changes. Weigh 3g of ground pork ball samples, wrap them with filter paper, place a 50ml centrifuge tube (put a small piece of cotton ball at the bottom), centrifuge at 6000r/min for 10min at 25°C, and then weigh the before and after mass.
保水性%=m1/m2×100%式中:m1:为离心前质量,m2:离心后的质量。Water retention %=m1/m2×100% In the formula: m1: mass before centrifugation, m2: mass after centrifugation.
(2)色泽分析(2) Color Analysis
将样品切成1em的薄片,进行色泽检测。采用1976年国际照明协会(CIE)制定的均匀色立体表色系统,检测结果以L*,a*,b*表示,L*表示明度指数,L*=100表示白色,L*=0表示黑色,中间有100个等级,代表不同的灰度;+a*表示红值,-a*表示绿值;+b*值表示黄值,-b*表示蓝值。Cut the sample into 1em slices for color testing. Adopt the uniform color three-dimensional colorimetric system formulated by the International Association of Illumination (CIE) in 1976, and the test results are expressed in L*, a*, b*, L* means lightness index, L*=100 means white, L*=0 means black , there are 100 levels in the middle, representing different gray levels; +a* indicates red value, -a* indicates green value; +b* indicates yellow value, -b* indicates blue value.
(3)质构检测(3) Texture detection
选择硬度、弹性、咀嚼性作为质构的检测指标。测试条件为:样品切成长2cm、宽2cm、高1cm,采用TPA模型,选用P/36R圆柱形不锈钢探头。测定参数设定为触发类型Button、触发力5.0g,测定速率3mm/s,测定中和测定后速率30.00mm/s、下压距离5.00mm。重复检测3次。Choose hardness, elasticity, chewiness as the detection index of texture. The test conditions are: the sample is cut into 2cm in length, 2cm in width and 1cm in height, adopts TPA model, and selects P/36R cylindrical stainless steel probe. The measurement parameters were set as trigger type Button, trigger force 5.0g, measurement speed 3mm/s, measurement speed 30.00mm/s during and after measurement, and pressing distance 5.00mm. Repeat the detection 3 times.
(4)感官评定(4) Sensory evaluation
具体地,以10位食品专业有经验的师生作为感官评定小组,对产品的色泽、香气、口感、切片性能进行感官评价,样品感官评价标准如表1所示:Specifically, 10 experienced teachers and students in food majors were used as the sensory evaluation team to conduct sensory evaluation on the color, aroma, taste, and slice performance of the product. The sensory evaluation standards of the samples are shown in Table 1:
表1Table 1
(5)保鲜性能测定(5) Determination of preservation performance
按照发明制备方法,分别添加0.6%的巢脾多糖、0.6%的茶多酚和空白组,做成三组不同的猪肉丸,进行猪肉丸保鲜性能的研究。将待测的三种猪肉丸于常温放置,每天分别检测三种猪肉丸的酸价和挥发性盐基氮(连续检测7天)。According to the preparation method of the invention, 0.6% honeycomb polysaccharide, 0.6% tea polyphenol and blank group were added respectively to make three groups of different pork balls, and the fresh-keeping performance of pork balls was studied. Place the three kinds of pork balls to be tested at normal temperature, and detect the acid value and volatile base nitrogen of the three kinds of pork balls every day (continuous detection for 7 days).
a、酸价测定(直接滴定法)a. Determination of acid value (direct titration method)
取5g肉置于250ml碘瓶中,加入30ml乙醚-乙醇(体积比2∶1)混合液,摇床45min,加三滴酚酞指示剂,用0.05N的KOH溶液滴定至出微红色且在30S内不消失,记下消耗的KOH溶液毫升数,结果以mg.KOH/g肉计。按以下公式计算:Take 5g of meat and place it in a 250ml iodine bottle, add 30ml of ether-ethanol (volume ratio 2:1) mixture, shake the table for 45min, add three drops of phenolphthalein indicator, titrate with 0.05N KOH solution until reddish and within 30S If it does not disappear, record the number of milliliters of KOH solution consumed, and the result is expressed in mg.KOH/g meat. Calculated according to the following formula:
V——滴定消耗KOH溶液体积mlV——The volume of KOH solution consumed by titration ml
N——氢氧化钾溶液当量浓度,0.05NN——Normal concentration of potassium hydroxide solution, 0.05N
W——肉制品质量,gW——the quality of meat products, g
56.1——KOH毫克当量56.1——KOH mg equivalent
b、挥发性盐基氮的测定b. Determination of volatile base nitrogen
根据GB/T 5009.44-2003半微量定氮法来进行试验。The test was carried out according to GB/T 5009.44-2003 semi-micro nitrogen determination method.
试样处理:将猪肉丸绞碎搅匀,取10.0g置于锥形瓶中,加100ml蒸馏水,不时振摇,浸渍30min后过滤,滤液置冰箱备用。Sample treatment: Mince the pork balls and mix well, take 10.0g and put it in a conical flask, add 100ml of distilled water, shake it from time to time, soak for 30min, then filter, and put the filtrate in the refrigerator for later use.
蒸馏滴定:将盛有10ml吸收液及5~6滴混合指示液的锥形瓶置于冷凝管下端,并使其下端插入吸收液的液面下,准确吸取5.0ml上树试样滤液于蒸馏器反应室内,加5ml氧化镁混合悬液(10g/L),迅速盖塞并加水以防漏气。通入蒸汽,进行蒸馏5min后停止,吸收液用盐酸标准滴定溶液(0.010mol/L)或硫酸标准滴定,终点至蓝紫色,同时做空白试验。Distillation titration: Place the conical flask containing 10ml of absorption solution and 5-6 drops of mixed indicator solution at the lower end of the condenser tube, insert the lower end under the liquid level of the absorption solution, and accurately draw 5.0ml of the tree sample filtrate in the distillation Add 5ml of magnesia mixed suspension (10g/L) to the reaction chamber of the reactor, quickly cover the plug and add water to prevent air leakage. Put in steam, stop after 5 minutes of distillation, and titrate the absorption solution with hydrochloric acid standard titration solution (0.010mol/L) or sulfuric acid standard until the end point reaches blue-purple, and do a blank test at the same time.
结果计算:试剂中挥发性盐基氮的含量按以下的公式计算(结果保留三位有效数字):Calculation of results: The content of volatile basic nitrogen in the reagent is calculated according to the following formula (the result retains three significant figures):
X——试样中挥发性盐基氮的含量,mg/100gX——The content of volatile basic nitrogen in the sample, mg/100g
V1——测定用样液消耗盐酸或硫酸标液体积,mlV 1 ——The volume of hydrochloric acid or sulfuric acid standard solution consumed by the sample solution for determination, ml
V2——测定用空白消耗盐酸或硫酸标液体积,mlV 2 ——The volume of hydrochloric acid or sulfuric acid standard solution consumed by the blank, ml
C——盐酸标液实际浓度,mol/LC——actual concentration of hydrochloric acid standard solution, mol/L
m——试样质量,gm—sample mass, g
上述各项检测和评定的结果如下:The results of the above tests and evaluations are as follows:
(1)不同巢脾多糖的脂肪替代量对肉丸的保水性的影响(1) Effect of fat replacement amount of different honeycomb polysaccharides on the water retention of meatballs
由图2可以看出,随着脂肪替代量的增加,产品的保水性呈先上升后下降的趋势。在脂肪替代量60%时保水性达到顶点97.13%。在脂肪替代量60%以内的产品保水性总体均大于空白样品(替代量为0)的保水性,证明巢脾多糖的添加可显著增加产品的保水性,源于巢脾多糖有增稠、稳定的作用,从而锁住猪肉丸中所含的水分,有效降低水分的流失。It can be seen from Figure 2 that with the increase of fat replacement, the water retention of the product first increases and then decreases. When the fat replacement amount is 60%, the water retention reaches the peak of 97.13%. The water retention of the products within 60% of the fat replacement amount is generally greater than that of the blank sample (the replacement amount is 0), which proves that the addition of nest spleen polysaccharide can significantly increase the water retention of the product, which is due to the thickening and stability of nest spleen polysaccharide The effect of locking the water contained in the pork balls, effectively reducing the loss of water.
(2)巢脾多糖的脂肪替代量对肉丸色差的影响(2) The effect of the fat replacement amount of honeycomb polysaccharide on the color difference of meatballs
由图3可知,多糖组的L*值和b*值均大于空白组,60%脂肪替代率以内的多糖组a*值也高于空白组,说明多糖的添加增加了猪肉丸的亮度、红度值及黄度值。猪肉丸的色泽是其感官品质的重要组成部分,亮度高、暖色值高的产品更易受到消费者的喜欢,因此适量的巢脾多糖的添加可改善产品的光度和色度,从而提高其感官品质。It can be seen from Figure 3 that the L* value and b* value of the polysaccharide group are greater than that of the blank group, and the a* value of the polysaccharide group within 60% of the fat replacement rate is also higher than that of the blank group, indicating that the addition of polysaccharide increases the brightness and redness of pork balls. degree and yellowness value. The color of pork balls is an important part of its sensory quality. Products with high brightness and high warm color value are more likely to be liked by consumers. Therefore, the addition of an appropriate amount of honeycomb polysaccharide can improve the luminosity and chroma of the product, thereby improving its sensory quality. .
(3)巢脾多糖的脂肪替代量对肉丸质构(弹性、硬度、咀嚼性)的影响(3) Effect of fat replacement amount of honeycomb polysaccharide on the texture (elasticity, hardness, chewiness) of meatballs
a、巢脾多糖的脂肪替代量对肉丸弹性的影响a. The effect of the fat replacement amount of honeycomb polysaccharide on the elasticity of meatballs
从图4a可以看出,随着脂肪替代量的提高,产品的弹性基本呈上升趋势,表明巢脾多糖的添加可增加猪肉丸的弹性。巢脾多糖对产品弹性的影响可能是巢脾多糖与肉糜结构蛋白之间作用的结果。It can be seen from Figure 4a that with the increase of fat replacement, the elasticity of the product basically showed an upward trend, indicating that the addition of honeycomb polysaccharide can increase the elasticity of pork balls. The effect of honeycomb polysaccharides on product elasticity may be the result of the interaction between honeycomb polysaccharides and minced meat structural proteins.
b、脂肪替代量比对肉丸硬度的影响b. Effect of fat replacement ratio on meatball hardness
从图4b可以看出,随着脂肪替代量不断增加,产品的硬度不断上升,多糖具有硬凝胶性,加之脂肪含量的减少,致使添加了巢脾多糖的猪肉丸硬度不断增加。而硬度过大会影响产品的口感,因此需控制脂肪替代量。It can be seen from Figure 4b that with the increasing amount of fat replacement, the hardness of the product continues to rise, and the polysaccharide has a hard gelatinity. In addition, the fat content decreases, resulting in the increasing hardness of pork balls added with honeycomb polysaccharide. Excessive hardness will affect the taste of the product, so the amount of fat replacement needs to be controlled.
c、脂肪替代量对肉丸咀嚼性的影响c. Effect of fat replacement amount on the chewiness of meatballs
从图4c可以看出,随着脂肪替代量的不断增加,产品的咀嚼性呈现不断上升的趋势,硬度的增大可能是咀嚼性增大的原因,进一步说明需合理控制脂肪替代量。It can be seen from Figure 4c that with the increasing amount of fat replacement, the chewiness of the product shows a rising trend, and the increase in hardness may be the reason for the increase in chewiness, further indicating that the amount of fat replacement needs to be controlled reasonably.
(4)感官评价(4) Sensory evaluation
感官评价结果如表2所示:The sensory evaluation results are shown in Table 2:
表2Table 2
从表2可以看出,随着脂肪替代量的增加,感官评定呈现先上升后下降的趋势。脂肪替代量60%以内,产品感官评价较好,随着脂肪替代量的进一步增加,产品的颜色香味和口感都迅速下降。巢脾多糖可替代猪肉丸中一定量的脂肪,在一定的脂肪替代量下巢脾多糖有利于改善肉丸的品质,但替代量过高也都会给产品风味产生负面影响。It can be seen from Table 2 that with the increase of fat replacement amount, the sensory evaluation showed a trend of rising first and then falling. When the fat replacement amount is within 60%, the sensory evaluation of the product is good. With the further increase of the fat replacement amount, the color, fragrance and mouthfeel of the product decrease rapidly. Nest spleen polysaccharide can replace a certain amount of fat in pork balls. Under a certain amount of fat replacement, nest spleen polysaccharide is beneficial to improve the quality of meatballs, but if the replacement amount is too high, it will also have a negative impact on the flavor of the product.
综上,蜜蜂巢脾多糖可有效改善低脂猪肉丸的保水性、弹性、硬度以及咀嚼性,而脂肪替量为60%的产品具有最佳的感官品质。In conclusion, honeycomb spleen polysaccharide can effectively improve the water retention, elasticity, hardness and chewiness of low-fat pork meatballs, and the product with 60% fat replacement has the best sensory quality.
(5)巢脾多糖对猪肉丸保鲜性能的影响(5) Effect of honeycomb polysaccharide on the fresh-keeping performance of pork balls
a、酸价测定(直接滴定法)a. Determination of acid value (direct titration method)
酸价测定(直接滴定法)结果如图5a所示。由图5a可以看出,随着储存时间的延长,三组产品的酸价值都呈现逐渐上升的趋势。添加巢脾多糖样品的酸价值增长趋势小于空白对照及茶多酚组,说明添加巢脾多糖的猪肉丸的脂肪酸败趋势减缓,从而可增加保藏时间。The results of acid value determination (direct titration) are shown in Figure 5a. It can be seen from Figure 5a that with the prolongation of storage time, the acid values of the three groups of products showed a gradual upward trend. The acid value growth trend of the sample added with honeycomb polysaccharide was smaller than that of the blank control and tea polyphenols group, indicating that the fatty acid decay trend of pork balls added with honeysuckle polysaccharide slowed down, which can increase the storage time.
b、挥发性盐基氮的测定b. Determination of volatile base nitrogen
挥发性盐基氮的测定结果如图5b所示。由图5b可以看出,随着储存时间的延长,三组样品的挥发性盐基氮值都呈现逐渐上升的趋势。添加巢脾多糖样品的挥发性盐基氮值增长趋势小于空白对照及茶多酚组。在储存的第4天,多糖组的挥发性盐基氮值≤15mg/100g,符合GB 2070-2005规定值,而茶多酚组与空白对照组的挥发性盐基氮值均超过了国标规定值。说明巢脾多糖的添加可有效抑制猪肉丸的腐败,从而可增加保藏时间。The measurement results of volatile basic nitrogen are shown in Fig. 5b. It can be seen from Figure 5b that with the prolongation of storage time, the volatile basic nitrogen values of the three groups of samples showed a gradual upward trend. The increase trend of volatile base nitrogen value of the sample added honeycomb polysaccharide was smaller than that of the blank control and tea polyphenol group. On the 4th day of storage, the volatile basic nitrogen value of the polysaccharide group was ≤15mg/100g, which was in line with the specified value of GB 2070-2005, while the volatile basic nitrogen value of the tea polyphenol group and the blank control group exceeded the national standard value. It shows that the addition of honeycomb polysaccharide can effectively inhibit the spoilage of pork meatballs, thus prolonging the storage time.
根据上述各项检测和评定的结果可以看出,蜜蜂巢脾多糖可替代部分猪肉丸中的脂肪,在60%以内的脂肪替代量下,可有效改善产品的色泽、弹性、硬度、咀嚼性以及感官品质。其中脂肪替代量60%的产品保水性最优,弹性、硬度及咀嚼性俱佳,符合制作健康美味的低脂猪肉丸的要求。同时巢脾多糖对猪肉丸的保鲜性能的研究结果表明,在观测天数内,添加巢脾多糖猪肉丸的挥发性盐基氮的值均小于空白对照组以及茶多酚组。在测量的第4天,添加巢脾多糖猪肉丸的挥发性盐基氮的值≤15mg/100g,符合GB 2070-2005规定,而添加茶多酚和空白样品的挥发性盐基氮的值都超过了国标规定值;添加巢脾多糖猪肉丸的酸价值均小于添加茶多酚猪肉丸及空白猪肉丸的酸价值。说明巢脾多糖不仅提高了低脂调理猪肉丸的感官品质特性,也延长了调理猪肉丸的储藏期。According to the results of the above tests and evaluations, it can be seen that honeycomb polysaccharides can replace part of the fat in pork balls, and can effectively improve the color, elasticity, hardness, chewiness and Sensory qualities. Among them, the product with 60% fat replacement has the best water retention, good elasticity, hardness and chewiness, which meets the requirements for making healthy and delicious low-fat pork balls. At the same time, the research results of the preservation performance of honeycomb polysaccharide on pork balls showed that within the observation days, the value of volatile base nitrogen of pork meatballs added with honeycomb polysaccharide was lower than that of the blank control group and the tea polyphenol group. On the 4th day of measurement, the value of volatile basic nitrogen of pork balls added with honeycomb polysaccharides was ≤15mg/100g, in line with the regulations of GB 2070-2005, while the values of volatile basic nitrogen of added tea polyphenols and blank samples were both It exceeded the national standard value; the acid value of pork balls added with honeycomb polysaccharides was lower than that of pork balls added with tea polyphenols and blank pork balls. It shows that nest spleen polysaccharide not only improves the sensory quality characteristics of low-fat conditioned pork meatballs, but also prolongs the storage period of conditioned pork meatballs.
本发明实施例提供的添加有巢脾多糖的低脂猪肉丸及其制作方法,采用蜜蜂巢脾多糖替代猪肉丸中的部分脂肪,巢脾多糖的添加能够有效改善猪肉丸的色泽、弹性、硬度、咀嚼性,保证猪肉丸的品质,还可以延长猪肉丸的储藏期。此外,蜜蜂巢脾中含有大量的蜂蜡,还含有丰富的生物活性成分如生物碱、有机酸、树脂、酶类、氨基酸、蛋白质、多糖、苷类以及昆虫激素等成分,从蜜蜂巢脾中提取的巢脾多糖是具有抗氧化、增强免疫力等生物学活性的高分子物质,从而使得添加有巢脾多糖的猪肉丸有益于人体健康。The low-fat pork balls added with honeycomb polysaccharide and its production method provided by the embodiment of the present invention use honeycomb polysaccharide to replace part of the fat in pork balls, and the addition of honeycomb polysaccharide can effectively improve the color, elasticity and hardness of pork balls , chewiness, ensure the quality of pork balls, and can also extend the storage period of pork balls. In addition, bee honeycomb contains a large amount of beeswax, and also contains rich biologically active components such as alkaloids, organic acids, resins, enzymes, amino acids, proteins, polysaccharides, glycosides and insect hormones, etc., which are extracted from honeycomb The nest spleen polysaccharide is a polymer substance with biological activities such as anti-oxidation and immunity enhancement, so that the pork balls added with nest spleen polysaccharide are beneficial to human health.
以上所述,仅为本发明的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,可轻易想到的变化或替换,都应涵盖在本发明的保护范围之内。因此,本发明的保护范围应该以权利要求的保护范围为准。The above is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Anyone skilled in the art can easily think of changes or substitutions within the technical scope disclosed in the present invention. All should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be determined by the protection scope of the claims.
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