CN106962716A - A kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables and preparation method thereof - Google Patents

A kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables and preparation method thereof Download PDF

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Publication number
CN106962716A
CN106962716A CN201710360206.9A CN201710360206A CN106962716A CN 106962716 A CN106962716 A CN 106962716A CN 201710360206 A CN201710360206 A CN 201710360206A CN 106962716 A CN106962716 A CN 106962716A
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ganoderma lucidum
juice
sprouted unpolished
unpolished rice
fruits
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CN106962716B (en
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罗建光
许旖旎
顾立众
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Jiangsu Food and Pharmaceutical Science College
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Jiangsu Food and Pharmaceutical Science College
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables and preparation method thereof, belong to technical field of food biotechnology.The ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables of the present invention prepares gained by 100 portions of ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, 10 portions of sucrose, 0.6 part of honey element, 0.15 part of potassium sorbate, 0.5 part of xanthans, 0.1 part of sodium alginate and 0.2 part of β ﹣ cyclodextrin.The ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables of the present invention is rich in GL-B, gamma aminobutyric acid and various vitamins, it is nutritious, human homergy can be promoted, strengthen passive protective physical fitness, blood glucose is adjusted, blood pressure is controlled, improves angiocardiopathy, liver protecting, promote sleep, be suitable for the crowds such as working clan, student, person in middle and old age and drink;And fresh orange-juice, cider, the carrot juice of addition etc., the bitterness of ganoderma lucidum in itself is reduced, mouthfeel is improved, adds fragrance so that user is more happy to drink, and nutritious, mouthfeel is good, suitable for people of all ages.

Description

A kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables and preparation method thereof
Technical field
The present invention relates to a kind of preparation method of beverage made of fruits or vegetables, and in particular to a kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables and its Preparation method, belongs to technical field of food biotechnology.
Background technology
Ganoderma lucidum is also known as red sesame, red ganoderma lucidum, polyporus lucidus, refreshing sesame, Lin Zhongling, fine jade treasure etc., is famous high medicinal true of China Bacterium and food resource, early in the Eastern Han Dynasty《Sheng Nong's herbal classic》With written by Ming Dynasty Li Shizhen (1518-1593 A.D.)《Compendium of Materia Medica》Described in, ganoderma lucidum has invigorating heart Gas, calms the nerves, beneficial lung qi, the health-care efficacy such as tonifying liver gas.Ganoderma lucidum is because its is mild-natured, gas is special, mildly bitter flavor, for enhancing human immunity Power, adjusts blood glucose, controls blood pressure, and adjuvant therapy chemicotherapy, liver protecting promotes to be respectively provided with significant curative effect in terms of sleep.Spirit Being mainly characterized by of its composition of sesame can suppress histamine, with the antianaphylactic effect of anti-inflammatory, in addition, GL-B can increase human body Immunologic function, to angiocardiopathy, hypotensive, it is hypoglycemic, improve blood fat etc. and have good effect.
Brown rice, is a kind of Wholegrain rice obtained after paddy sloughs rice husk through hulling machine, richer compared with common rice There are many vitamins, mineral matter and dietary fiber, be considered to be a kind of green healthy food.Sprouted unpolished rice is brown rice by leaching After bubble and germination, make brown rice enrichment that there is the material of physiologically active to human body, particularly alpha-aminobutyric acid content is dramatically increased. γ-aminobutyric acid is a kind of nonprotein amino acid, is widely present in animal and plant body, as the important neurotransmitter of human body, With hypotensive, improve brain blood circulation, stable spirit, be good for the sharp liver of kidney and improve the physiological actions such as lipid-metabolism.Rich in γ- There is the food of aminobutyric acid good immunizing health to act on, and be a kind of functional food, and it is researched and developed also therefore by weight Depending on.
The ganoderma lucidum product of a multitude of names is occurred in that on the market at present, most techniques are directly that ganoderma lucidum fruitbody is direct The processing modes such as pulverizing processing, section are obtained, and active constituent content is relatively low in the ganoderma lucidum food finally obtained, fermentation method production spirit Sesame active material has the advantages that with short production cycle, yield is high, steady quality, simultaneously compared with Ganoderma Lucidum fructification Ganoderma polyoses content is high in zymotic fluid, it is therefore desirable to which further in-depth fermentation method produces the exploitation and application study of ganoderma lucidum industry, And current glossy ganoderma health beverage heavy bitter taste on the market, poor taste is difficult to be esthetically acceptable to the consumers, and is that this present invention proposes a kind of spirit Sesame sprouted unpolished rice beverage made of fruits or vegetables and preparation method thereof, the problem of to solve to mention in above-mentioned background technology.
The content of the invention
In view of the above-mentioned problems of the prior art, the present invention provides a kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables and its preparation Method, the ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables is rich in GL-B, γ-aminobutyric acid and various vitamins, can promote human body just Often metabolism, strengthens passive protective physical fitness, is suitable for the crowds such as working clan, student, person in middle and old age and drinks.
To achieve these goals, a kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables of the invention, includes the raw material of following parts by weight: 100 parts of ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, 10 parts of sucrose, 0.6 part of honey element, 0.15 part of potassium sorbate, 0.5 part of xanthans, 0.1 Part sodium alginate, 0.2 part of β ﹣ cyclodextrin.
Further, 100 portions of ganoderma lucidums sprouted unpolished rice fruits and vegetables juice is made by the raw material of following parts by weight, 70 portions of ganoderma lucidums Sprouted unpolished rice zymotic fluid, 12 portions of fresh orange-juices, 9.6 portions of ciders, 8.4 portions of carrot juices.
A kind of preparation method of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables, comprises the following steps:
(1) preparation of ganoderma lucidum sprouted unpolished rice zymotic fluid:
1. actication of culture:Inclined-plane culture based component is peptone 0.5%, glucose 1%, NaCl 0.5%, CaCO3 0.02%th, agar 1.5%, pH is adjusted to 7.2~7.4;One piece of 0.5cm is taken from ganoderma lucidum CICC14042 parent species inclined-plane2The bacterium of size Block is inoculated on slant medium, and is placed on culture 7 days in 25 DEG C of constant incubators together, and activated inclined plane strain is made;
2. seed culture:Seed culture based component is corn flour 5%, sucrose 1%, maltose 1%, in 250mL triangular flasks Room temperature is cooled to after middle loading 100mL seed culture mediums, autoclaving, in 2~3 pieces of 5mm of access under aseptic condition2Activation it is oblique Face strain, is then placed in shaking flask together, is cultivated 7 days under the conditions of 28 DEG C, 180 revs/min, and liquid lucidum strain is made;
3. liquid fermentation:Liquid fermentation medium composition is germination brown rice powder 3%, barley flour of germination 1.5%, germination soya bean Powder 2%, MgSO40.05%th, KH2PO40.05%th, K2HPO40.05%, the training of 200mL liquid fermentations is loaded in 500mL triangular flasks Support base, be cooled to room temperature after autoclaving, under aseptic condition, liquid lucidum strain is accessed by 5% inoculum concentration, 28 DEG C, 180 Fermented and cultured 7 days under the conditions of rev/min, are made ganoderma lucidum sprouted unpolished rice fermentation liquid;
4. ganoderma lucidum sprouted unpolished rice fermentation liquid is centrifuged:Ganoderma lucidum sprouted unpolished rice fermentation liquid is entered under the conditions of 4000 revs/min Row centrifugation 20min, obtains ganoderma lucidum sprouted unpolished rice zymotic fluid stoste and mycelium, wherein detection ganoderma lucidum sprouted unpolished rice zymotic fluid stoste The content of middle GL-B and γ-aminobutyric acid, and the allotment GL-B into obtained ganoderma lucidum sprouted unpolished rice zymotic fluid that adds water Content >=450mg/L, alpha-aminobutyric acid content >=300mg/L is standby.
(2) preparation of fresh orange-juice:
1. sort:90% or so maturation is selected, bright in colour, the pure strong sweet orange of fruity rejects pest and disease damage fruit, prematurity Really, withered fruit, injured fruit;
2. the sweet orange screened is washed away into dust, with 0.02% hypochlorite disinfectant 10-20 minutes, rinses dry with drinking water Only;
3. pericarp is peeled off, pulp is cut into small pieces, (the weight of sweet orange fruit pulp and solution is put into 0.1% citric acid solution Amount is than being 100: 70) soaks 30 minutes, fresh orange-juice is made in mashing, squeezing, filtering.
(3) preparation of cider:
1. the ripe, apple of no disease and pests harm is selected, dust is washed away, with 0.02% hypochlorite disinfectant 10-20 minutes, with drink Rinsed well with water;
2. remove the peel, be enucleated, be cut into 2cm or so fritter, be put into juice extractor, be put into 0.2% anti-citric acid solution (the weight ratio of apple particle and solution is 100: 70), is beaten, is squeezed, is filtered, cider is made.
(4) preparation of carrot juice:
1. loose, no disease and pests harm is selected, the scarlet carrot of color washes away soil and dust, disappeared with 0.02% sodium hypochlorite It is malicious 10-20 minutes, rinsed well with drinking water;
2. the carrot peeling after cleaning, cutting into slices when vapor (steam) temperature is 123 DEG C soften removes fishy smell, during softening Between 10 seconds, (the weight ratio of carrot slice and solution be 100: 70) is put into 0.5% sodium ascorbate solution after terminating, is carried out Mashing, squeezing, filtering, are made carrot juice.
(5) preparation of compound fruit and vegetable juice:
1. taking fresh orange-juice, cider, carrot juice to be well mixed, (the weight ratio of fresh orange-juice, cider and carrot juice is 100 : 80: 70), mixing juice is made;
2. juice, pectase, cellulase (weight ratio is 100: 0.7: 0.2) will be mixed to be well mixed and digested, enzyme Temperature 50 C is solved, after the completion of enzymolysis, by the mixing juice after enzymolysis through the enzyme that goes out, cooling, composite fruit is made in enzymolysis time 3 hours Vegetable juice.
(6) preparation of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables:
1. by above-mentioned ganoderma lucidum sprouted unpolished rice zymotic fluid, compound fruit and vegetable juice it is well mixed (ganoderma lucidum sprouted unpolished rice zymotic fluid with it is multiple The weight ratio for closing Juice is 100:30) ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, is made;
2. by above-mentioned ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, sucrose, honey element, potassium sorbate, xanthans, sodium alginate, β ﹣ (weight ratio is 100 to cyclodextrin:10:0.6:0.15:0.5:0.1:0.2) after, being well mixed, drink raw stock is made;
3. by drink raw stock in 65~75 DEG C of temperature, pressure under conditions of 20~25MPa, homogeneous 2 times, the production after homogeneous Product are handled 10 minutes under the conditions of 85~90 DEG C, are subsequently cooled to 30 DEG C, aseptically filling, after the assay was approved, normal temperature Lower storage, produces ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables.
The beneficial effects of the invention are as follows:The ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables of the present invention (is not less than rich in GL-B 300mg/L), γ-aminobutyric acid (being not less than 200mg/L) and various vitamins, nutritious, can promote human homergy, Strengthen passive protective physical fitness, adjust blood glucose, control blood pressure, improve angiocardiopathy, liver protecting, promote sleep, be suitable for working The crowds such as race, student, person in middle and old age drink;And fresh orange-juice, cider, the carrot juice of addition etc., reduce the hardship of ganoderma lucidum in itself It is puckery, mouthfeel is improved, fragrance is added so that user is more happy to drink, and nutritious, mouthfeel is good, suitable for people of all ages.
Embodiment
With reference to specific embodiment, the invention will be further described, it is clear that described embodiment is only this hair Bright a part of embodiment, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art are not having There is the every other embodiment made and obtained under the premise of creative work, belong to the scope of protection of the invention.
Embodiment 1
A kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables, includes the raw material of following parts by weight:
100 parts of ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, 10 parts of sucrose, 0.6 part of honey element, 0.15 part of potassium sorbate, 0.5 part of Huang Virgin rubber, 0.1 part of sodium alginate, 0.2 part of β ﹣ cyclodextrin;Wherein 100 portions ganoderma lucidum sprouted unpolished rice fruits and vegetables juice are by following parts by weight Raw material is made:70 portions of ganoderma lucidum sprouted unpolished rice zymotic fluids, 12 portions of fresh orange-juices, 9.6 portions of ciders, 8.4 portions of carrot juices.
A kind of preparation method of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables, comprises the following steps:
(1) preparation of ganoderma lucidum sprouted unpolished rice zymotic fluid:
1. actication of culture:Inclined-plane culture based component is peptone 0.5%, glucose 1%, NaCl 0.5%, CaCO3 0.02%th, agar 1.5%, pH is adjusted to 7.2~7.4;One piece of 0.5cm is taken from ganoderma lucidum CICC14042 parent species inclined-plane2The bacterium of size Block is inoculated on slant medium, and is placed on culture 7 days in 25 DEG C of constant incubators together, and activated inclined plane strain is made;
2. seed culture:Seed culture based component is corn flour 5%, sucrose 1%, maltose 1%, in 250mL triangular flasks Room temperature is cooled to after middle loading 100mL seed culture mediums, autoclaving, in 2~3 pieces of 5mm of access under aseptic condition2Activation it is oblique Face strain, is then placed in shaking flask together, is cultivated 7 days under the conditions of 28 DEG C, 180 revs/min, and liquid lucidum strain is made;
3. liquid fermentation:Liquid fermentation medium composition is germination brown rice powder 3%, barley flour of germination 1.5%, germination soya bean Powder 2%, MgSO40.05%th, KH2PO40.05%th, K2HPO40.05%, the training of 200mL liquid fermentations is loaded in 500mL triangular flasks Support base, be cooled to room temperature after autoclaving, under aseptic condition, liquid lucidum strain is accessed by 5% inoculum concentration, 28 DEG C, 180 Fermented and cultured 7 days under the conditions of rev/min, are made ganoderma lucidum sprouted unpolished rice fermentation liquid;
4. ganoderma lucidum sprouted unpolished rice fermentation liquid is centrifuged:Ganoderma lucidum sprouted unpolished rice fermentation liquid is entered under the conditions of 4000 revs/min Row centrifugation 20min, obtains ganoderma lucidum sprouted unpolished rice zymotic fluid stoste and mycelium, wherein detection ganoderma lucidum sprouted unpolished rice zymotic fluid stoste The content of middle GL-B and γ-aminobutyric acid, and the allotment GL-B into obtained ganoderma lucidum sprouted unpolished rice zymotic fluid that adds water Content >=450mg/L, alpha-aminobutyric acid content >=300mg/L is standby.
(2) preparation of fresh orange-juice:
1. sort:90% or so maturation is selected, bright in colour, the pure strong sweet orange of fruity rejects pest and disease damage fruit, prematurity Really, withered fruit, injured fruit;
2. the sweet orange screened is washed away into dust, with 0.02% hypochlorite disinfectant 10-20 minutes, rinses dry with drinking water Only;
3. pericarp is peeled off, pulp is cut into small pieces, (the weight of sweet orange fruit pulp and solution is put into 0.1% citric acid solution Amount is than being 100: 70) soaks 30 minutes, fresh orange-juice is made in mashing, squeezing, filtering.
(3) preparation of cider:
1. the ripe, apple of no disease and pests harm is selected, dust is washed away, with 0.02% hypochlorite disinfectant 10-20 minutes, with drink Rinsed well with water;
2. remove the peel, be enucleated, be cut into 2cm or so fritter, be put into juice extractor, be put into 0.2% anti-citric acid solution (the weight ratio of apple particle and solution is 100: 70), is beaten, is squeezed, is filtered, cider is made.
(4) preparation of carrot juice:
1. loose, no disease and pests harm is selected, the scarlet carrot of color washes away soil and dust, disappeared with 0.02% sodium hypochlorite It is malicious 10-20 minutes, rinsed well with drinking water;
2. the carrot peeling after cleaning, cutting into slices when vapor (steam) temperature is 123 DEG C soften removes fishy smell, during softening Between 10 seconds, (the weight ratio of carrot slice and solution be 100: 70) is put into 0.5% sodium ascorbate solution after terminating, is carried out Mashing, squeezing, filtering, are made carrot juice.
(5) preparation of compound fruit and vegetable juice:
1. taking fresh orange-juice, cider, carrot juice to be well mixed, (the weight ratio of fresh orange-juice, cider and carrot juice is 100 : 80: 70), mixing juice is made;
2. will mixing juice, pectase, cellulase (mixing juice, pectase, cellulase weight ratio be 100: 0.7: 0.2) it is well mixed and is digested, 50 DEG C of hydrolysis temperature, enzymolysis time 3 hours, after the completion of enzymolysis, by the mixing juice after enzymolysis Through the enzyme that goes out, cooling, compound fruit and vegetable juice is made.
(6) preparation of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables:
1. by above-mentioned ganoderma lucidum sprouted unpolished rice zymotic fluid, compound fruit and vegetable juice it is well mixed (ganoderma lucidum sprouted unpolished rice zymotic fluid with it is multiple The weight ratio for closing Juice is 100: 30), ganoderma lucidum sprouted unpolished rice fruits and vegetables juice is made;
2. by above-mentioned ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, sucrose, honey element, potassium sorbate, xanthans, sodium alginate, β ﹣ (weight ratio is 100 to cyclodextrin:10:0.6:0.15:0.5:0.1:0.2) after, being well mixed, drink raw stock is made;
3. by drink raw stock in 65~75 DEG C of temperature, pressure under conditions of 20~25MPa, homogeneous 2 times, the production after homogeneous Product are handled 10 minutes under the conditions of 85~90 DEG C, are subsequently cooled to 30 DEG C, aseptically filling, after the assay was approved, normal temperature Lower storage, produces ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables.
In summary, ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables of the invention rich in GL-B (being not less than 300mg/L), γ- Aminobutyric acid (being not less than 200mg/L) and various vitamins, it is nutritious, human homergy can be promoted, strengthen body resistance Power, adjusts blood glucose, controls blood pressure, improves angiocardiopathy, liver protecting, promotes sleep, be suitable for working clan, student, in it is old The crowds such as year drink;And fresh orange-juice, cider, the carrot juice of addition etc., the bitterness of ganoderma lucidum in itself is reduced, mouthfeel is improved, Add fragrance so that user is more happy to drink, and nutritious, mouthfeel is good, suitable for people of all ages.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding And modification, the scope of the present invention is defined by the appended.

Claims (3)

1. a kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables, it is characterised in that include the raw material of following parts by weight:100 parts of ganoderma lucidum germinations Brown rice fruits and vegetables juice, 10 portions of sucrose, 0.6 part of honey element, 0.15 part of potassium sorbate, 0.5 part of xanthans, 0.1 part of sodium alginate, 0.2 part of β ﹣ cyclodextrin.
2. a kind of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables according to claim 1, it is characterised in that 100 portions of ganoderma lucidums hair Bud brown rice fruits and vegetables juice is made by the raw material of following parts by weight, 70 portions of ganoderma lucidum sprouted unpolished rice zymotic fluids, 12 parts of fresh orange-juices, 9.6 parts Cider, 8.4 portions of carrot juices.
3. a kind of preparation method of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables, it is characterised in that comprise the following steps:
(1) preparation of ganoderma lucidum sprouted unpolished rice zymotic fluid:
1. actication of culture:Inclined-plane culture based component is peptone 0.5%, glucose 1%, NaCl 0.5%, CaCO30.02%th, Agar 1.5%, pH is adjusted to 7.2~7.4;One piece of 0.5cm is taken from ganoderma lucidum CICC14042 parent species inclined-plane2The fungus block inoculation of size Onto slant medium, and culture 7 days in 25 DEG C of constant incubators are placed on together, activated inclined plane strain is made;
2. seed culture:Seed culture based component is corn flour 5%, sucrose 1%, maltose 1%, is filled in 250mL triangular flasks Room temperature is cooled to after entering 100mL seed culture mediums, autoclaving, in 2~3 pieces of 5mm of access under aseptic condition2Activated inclined plane bacterium Kind, then it is placed in shaking flask, is cultivated 7 days under the conditions of 28 DEG C, 180 revs/min together, liquid lucidum strain is made;
3. liquid fermentation:Liquid fermentation medium composition is germination brown rice powder 3%, barley flour of germination 1.5%, germination analysis for soybean powder 2%th, MgSO40.05%th, KH2PO40.05%th, K2HPO40.05%, 200mL liquid fermentation and cultures are loaded in 500mL triangular flasks Be cooled to room temperature after base, autoclaving, under aseptic condition, liquid lucidum strain accessed by 5% inoculum concentration, 28 DEG C, 180 Fermented and cultured 7 days under the conditions of rev/min, are made ganoderma lucidum sprouted unpolished rice fermentation liquid;
4. ganoderma lucidum sprouted unpolished rice fermentation liquid is centrifuged:Ganoderma lucidum sprouted unpolished rice fermentation liquid is carried out under the conditions of 4000 revs/min from Heart 20min, obtains ganoderma lucidum sprouted unpolished rice zymotic fluid stoste and mycelium, wherein spirit in detection ganoderma lucidum sprouted unpolished rice zymotic fluid stoste The content of sesame polysaccharide and γ-aminobutyric acid, and the allotment ganoderma polyoses content into obtained ganoderma lucidum sprouted unpolished rice zymotic fluid that adds water >=450mg/L, alpha-aminobutyric acid content >=300mg/L is standby.
(2) preparation of fresh orange-juice:
1. sort:Select 90% or so maturation, bright in colour, the pure strong sweet orange of fruity, reject pest and disease damage fruit, crude fruit, Withered fruit, injured fruit;
2. the sweet orange screened is washed away into dust, with 0.02% hypochlorite disinfectant 10-20 minutes, rinsed well with drinking water;
3. pericarp is peeled off, pulp is cut into small pieces, (the weight ratio of sweet orange fruit pulp and solution is put into 0.1% citric acid solution For 100:70) soak 30 minutes, fresh orange-juice is made in mashing, squeezing, filtering.
(3) preparation of cider:
1. the ripe, apple of no disease and pests harm is selected, dust is washed away, with 0.02% hypochlorite disinfectant 10-20 minutes, drinking water is used Rinse well;
2. remove the peel, be enucleated, be cut into 2cm or so fritter, be put into juice extractor, be put into (apple in 0.2% anti-citric acid solution The weight ratio of particle and solution is 100:70), it is beaten, squeezed, filtered, cider is made.
(4) preparation of carrot juice:
1. loose, no disease and pests harm is selected, the scarlet carrot of color washes away soil and dust, uses 0.02% hypochlorite disinfectant 10-20 minutes, rinsed well with drinking water;
2. the carrot peeling after cleaning, cutting into slices when vapor (steam) temperature is 123 DEG C soften removes fishy smell, softens the time 10 Second, be put into after terminating in 0.5% sodium ascorbate solution (the weight ratio of carrot slice and solution be 100:70), be beaten, Squeezing, filtering, are made carrot juice.
(5) preparation of compound fruit and vegetable juice:
1. taking fresh orange-juice, cider, carrot juice to be well mixed, (the weight ratio of fresh orange-juice, cider and carrot juice is 100:80: 70) mixing juice, is made;
2. juice, pectase, cellulase will be mixed, and (weight ratio is 100:0.7:0.2) it is well mixed and is digested, enzymolysis temperature After the completion of enzymolysis, by the mixing juice after enzymolysis through the enzyme that goes out, cooling, compound fruit and vegetable juice is made in 50 DEG C of degree, enzymolysis time 3 hours.
(6) preparation of ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables:
1. above-mentioned ganoderma lucidum sprouted unpolished rice zymotic fluid, compound fruit and vegetable juice are well mixed (ganoderma lucidum sprouted unpolished rice zymotic fluid and composite fruit The weight ratio of vegetable juice is 100:30) ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, is made;
2. above-mentioned ganoderma lucidum sprouted unpolished rice fruits and vegetables juice, sucrose, honey element, potassium sorbate, xanthans, sodium alginate, β ﹣ rings are pasted (weight ratio is 100 to essence:10:0.6:0.15:0.5:0.1:0.2) after, being well mixed, drink raw stock is made;
3. by drink raw stock in 65~75 DEG C of temperature, pressure under conditions of 20~25MPa, homogeneous 2 times, the product after homogeneous exists Handled 10 minutes under the conditions of 85~90 DEG C, be subsequently cooled to 30 DEG C, it is aseptically filling, after the assay was approved, store under normal temperature Hide, produce ganoderma lucidum sprouted unpolished rice beverage made of fruits or vegetables.
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