CN106957808A - A kind of probiotics for cynoglossus semilaevis cultivation - Google Patents
A kind of probiotics for cynoglossus semilaevis cultivation Download PDFInfo
- Publication number
- CN106957808A CN106957808A CN201710335519.9A CN201710335519A CN106957808A CN 106957808 A CN106957808 A CN 106957808A CN 201710335519 A CN201710335519 A CN 201710335519A CN 106957808 A CN106957808 A CN 106957808A
- Authority
- CN
- China
- Prior art keywords
- probiotics
- lactobacillus
- bacillus
- fermentation
- cynoglossus semilaevis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of probiotics for cynoglossus semilaevis cultivation; its be by the excellent bacillus of upgrowth situation and low temperature lactobacillus respectively after culture, fermentation, centrifugation; isolated thalline is added in the protective agent containing xylo-oligosaccharide, beta glucan, trehalose and skimmed milk and mixed, then the probiotics is made through vacuum freeze drying.Gained probiotics can not only suppress the growth of harmful pathogenic bacteria in Cynoglossus semilaevis body, moreover it is possible to remove ammonia nitrogen and nitrite, improve breeding water body environment, may advantageously facilitate the growth of Cynoglossus semilaevis, improve aquatic seedling survival rate.
Description
Technical field
The invention belongs to microbial preparation field, and in particular to a kind of probiotics for cynoglossus semilaevis cultivation.
Background technology
Probiotics is a kind of novel product of substitute antibiotics, with its noresidue, have no drug resistance, have no toxic side effect,
The advantages of effect is notable is widely used in the fields such as aquaculture, animal-breeding, planting industry and disease treatment, with great
Economy and social effect.
Lactobacillus, as beneficial bacterium apply earliest, with the longest history, is that a class can decompose carbohydrate to produce
Lactogenesis acid is the gram-positive bacteria of main metabolites.Such Pseudomonas is the microflora in animal intestinal tract, is colonized naturally
In alimentary canal, generation low energy aliphatic acid reduction pH value can be metabolized, so as to play inhibitory action to the harmful microorganism in enteron aisle, is adjusted
Whole gut flora balance, promotes body to digest and assimilate nutriment, improves the immunocompetence of body.Lactobacillus is applied to
Aquaculture industry is an effective way for solving the low problem of aquatic products survival rate.
There is quantity seldom in bacillus, but bacillus can form gemma in enteron aisle, in drying, high pressure, height
Viability in the adverse circumstances such as temperature, low temperature is stronger, and the physiology course such as can be sprouted, grown and be bred in enteron aisle, and
With a variety of efficient enzymatic effects.Bacillus can rapidly in degrading cultivation water various organic matters, including residual bait
Organic matter in material, the excreta of aquatic livestock, dead biological residuum and bottom of pond mud, in addition, moreover it is possible to reduce water
The content of ammonia nitrogen and nitrite nitrogen in body, the content for increasing dissolved oxygen, and then water quality is effectively improved, build good cultivation
Ecological environment.Bacillus is applied to the effective scheme that aquaculture industry is solution antibiotic pollution problem.
The content of the invention
It is an object of the invention to provide a kind of probiotics for cynoglossus semilaevis cultivation.Gained probiotics was both
Cynoglossus semilaevis survival rate of seedling can be improved, cultivation water can be improved again, there are wide market prospects in terms of aquaculture.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of probiotics for cynoglossus semilaevis cultivation, its preparation method comprises the following steps：
1）Bacillus and the culture of low temperature lactobacillus：Bacillus and lactobacillus strain are taken from test tube slant culture medium
Go out, be inoculated into the conical flask equipped with fluid nutrient medium, be subsequently placed in shaking table respectively, in 20 DEG C, 150-250r/min conditions
Lower culture 24h；
2）By step 1）Bacillus bacterium solution and lactobacillus bacterium solution in logarithmic phase are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature is 20 DEG C, and fermentation time is 48-72h；
3）By step 2）Fermentation of bacillus liquid and lactobacillus ferment liquid in logarithmic phase are centrifuged respectively at 10000r/min
20min, with separating thallus and supernatant；Gained thalline is added in protective agent jointly and stirred and evenly mixed, then through vacuum freeze drying
The probiotics is made.
Step 1）Liquid Culture based formulas used by middle bacillus is：5-8g containing peptone in per 1000mL distilled water,
Dusty yeast 1-3g；PH value 7.6-7.8, uses the preceding 15-20min that sterilized in 121 DEG C；Fluid nutrient medium used by lactobacillus is trained for MRS
Base is supported, the preceding 15-20min that sterilized in 121 DEG C is used.
Step 2）The inoculum concentration of middle lactobacillus bacterium solution and lactobacillus bacterium solution is 2vol%, both fermented and cultured basigamys used
Fang Wei：Soluble-containing starch 3-3.5g, peptone 10-12g, yeast extract 3-5g, KH in per 1000mL distilled water2PO4 1.5-4g、
KHPO4 2-4g、MgSO4 0.1-1g、ZnCl21-3g；PH value 7.4-7.6, uses the preceding 15-20min that sterilized in 121 DEG C.
Step 3）Described in protective agent be made up of xylo-oligosaccharide, beta glucan, trehalose, skimmed milk and water；Wherein, it is oligomeric
The concentration of xylose and beta glucan is 2.0g/L, and the concentration of trehalose is 5wt%, and the concentration of skimmed milk is 20wt%.
The probiotics can be used for the cultivation of Cynoglossus semilaevis as feed addictive.
Low temperature lactobacillus has excellent enzymatic productivity, and under cryogenic, enzymatic productivity is not decreased obviously.Addition
The feed of low temperature lactobacillus can dramatically increase the body weight of Cynoglossus semilaevis, improve the digestion power of Cynoglossus semilaevis.
Bacillus has the features such as moisture retention is strong, organic matter decomposition power is strong, antibacterial harmful power of going out is strong, and can produce what is enriched
Product is metabolized, such as a variety of organic acids, digestive ferment can suppress the harmful microbe growth and breeding such as harmful bacteria, pathogen, and
The content of ammonia nitrogen and nitrite in water can be efficiently reduced, and then improves water quality, improve breeding water body microenvironment.
The probiotics that bacillus and low temperature lactobacillus are mixed with by the present invention, can play feature collaboration work
With, from ecological angle, breeding water body ammonia-nitrogen content is reduced using microorganism, with it is natural, nontoxic, have no side effect, without dirt
Dye, noresidue, safe and reliable superiority；And improve water quality using beneficial microbe, and suppress pathogen, reduce disease, can
The growth of Cynoglossus semilaevis is effectively promoted, the survival rate of its seedling is improved.
The beneficial effects of the present invention are：Gained probiotics of the invention can direct plunge into foster as aquaculture feed
Grow in water body, it has the effect of good improvement breeding water body microenvironment, can reduce breeding water body ammonia-nitrogen content, and ammonia nitrogen is removed
Rate is up to 65%, and the clearance of nitrite can promote aquatic biological to digest and assimilate nutriment up to 75%, significantly carry
The rate of body weight gain of high Cynoglossus semilaevis and the survival rate of seedling.
Brief description of the drawings
Fig. 1 is ammonia nitrogen clearance rate change curve of the probiotics of the present invention under different time to breeding water body.
Fig. 2 is nitrite clearance rate change curve of the probiotics of the present invention under different time to breeding water body.
Embodiment
In order that content of the present invention easily facilitates understanding, with reference to embodiment to of the present invention
Technical scheme is described further, but the present invention is not limited only to this.
Embodiment 1
A kind of probiotics for cynoglossus semilaevis cultivation, its preparation method comprises the following steps：
1）Bacillus and the culture of low temperature lactobacillus：Bacillus and lactobacillus strain are taken from test tube slant culture medium
Go out, be inoculated into the conical flask equipped with fluid nutrient medium, be subsequently placed in shaking table respectively, trained under the conditions of 20 DEG C, 150r/min
Support 24h；
2）By step 1）Bacillus bacterium solution and lactobacillus bacterium solution in logarithmic phase are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature is 20 DEG C, and fermentation time is 72h；
3）By step 2）Fermentation of bacillus liquid and lactobacillus ferment liquid in logarithmic phase are centrifuged respectively at 10000r/min
20min, with separating thallus and supernatant；Gained thalline is added in the aqueous solution containing xylo-oligosaccharide and beta glucan jointly and mixed
It is even, trehalose is added, stir obtained suspension, be eventually adding skimmed milk as matrix, be made through vacuum freeze drying
The probiotics.
Step 1）Liquid Culture based formulas used by middle bacillus is：5g containing peptone, ferment in per 1000mL distilled water
Female powder 1g；PH value 7.6, uses the preceding 20min that sterilized in 121 DEG C；Fluid nutrient medium used by lactobacillus is MRS culture mediums, before use
Sterilize 20min in 121 DEG C.
Step 2）The inoculum concentration of middle lactobacillus bacterium solution and lactobacillus bacterium solution is 2vol%, both fermented and cultured basigamys used
Fang Wei：Soluble-containing starch 3g, peptone 10g, yeast extract 3g, KH in per 1000mL distilled water2PO4 1.5g、KHPO4 2g、
MgSO4 0.1g、ZnCl21g；PH value 7.6, uses the preceding 15min that sterilized in 121 DEG C.
Step 3）The concentration of xylo-oligosaccharide and beta glucan is 2.0g/L in mixed liquor, and the concentration of trehalose is 5wt%,
The concentration of skimmed milk is 20wt%.
Embodiment 2
A kind of probiotics for cynoglossus semilaevis cultivation, its preparation method comprises the following steps：
1）Bacillus and the culture of low temperature lactobacillus：Bacillus and lactobacillus strain are taken from test tube slant culture medium
Go out, be inoculated into the conical flask equipped with fluid nutrient medium, be subsequently placed in shaking table respectively, trained under the conditions of 20 DEG C, 200r/min
Support 24h；
2）By step 1）Bacillus bacterium solution and lactobacillus bacterium solution in logarithmic phase are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature is 20 DEG C, and fermentation time is 60h；
3）By step 2）Fermentation of bacillus liquid and lactobacillus ferment liquid in logarithmic phase are centrifuged respectively at 10000r/min
20min, with separating thallus and supernatant；Gained thalline is added in the aqueous solution containing xylo-oligosaccharide and beta glucan jointly and mixed
It is even, trehalose is added, stir obtained suspension, be eventually adding skimmed milk as matrix, be made through vacuum freeze drying
The probiotics.
Step 1）Liquid Culture based formulas used by middle bacillus is：6g containing peptone, ferment in per 1000mL distilled water
Female powder 2g；PH value 7.4, uses the preceding 18min that sterilized in 121 DEG C；Fluid nutrient medium used by lactobacillus is MRS culture mediums, before use
Sterilize 18min in 121 DEG C.
Step 2）The inoculum concentration of middle lactobacillus bacterium solution and lactobacillus bacterium solution is 2vol%, both fermented and cultured basigamys used
Fang Wei：Soluble-containing starch 3.2g, peptone 11g, yeast extract 4g, KH in per 1000mL distilled water2PO4 2.5g、KHPO4 3g、
MgSO4 0.6g、ZnCl22g；PH value 7.5, uses the preceding 18min that sterilized in 121 DEG C.
Step 3）The concentration of xylo-oligosaccharide and beta glucan is 2.0g/L in mixed liquor, and the concentration of trehalose is 5wt%,
The concentration of skimmed milk is 20wt%.
Embodiment 3
A kind of probiotics for cynoglossus semilaevis cultivation, its preparation method comprises the following steps：
1）Bacillus and the culture of low temperature lactobacillus：Bacillus and lactobacillus strain are taken from test tube slant culture medium
Go out, be inoculated into the conical flask equipped with fluid nutrient medium, be subsequently placed in shaking table respectively, in 20 DEG C, 150-250r/min conditions
Lower culture 24h；
2）By step 1）Bacillus bacterium solution and lactobacillus bacterium solution in logarithmic phase are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature is 20 DEG C, and fermentation time is 48h；
3）By step 2）Fermentation of bacillus liquid and lactobacillus ferment liquid in logarithmic phase are centrifuged respectively at 10000r/min
20min, with separating thallus and supernatant；Gained thalline is added in the aqueous solution containing xylo-oligosaccharide and beta glucan jointly and mixed
It is even, trehalose is added, stir obtained suspension, be eventually adding skimmed milk as matrix, be made through vacuum freeze drying
The probiotics.
Step 1）Liquid Culture based formulas used by middle bacillus is：8g containing peptone, ferment in per 1000mL distilled water
Female powder 3g；PH value 7.8, uses the preceding 20min that sterilized in 121 DEG C；Fluid nutrient medium used by lactobacillus is MRS culture mediums, before use
Sterilize 20min in 121 DEG C.
Step 2）The inoculum concentration of middle lactobacillus bacterium solution and lactobacillus bacterium solution is 2vol%, both fermented and cultured basigamys used
Fang Wei：Soluble-containing starch 3.5g, peptone 12g, yeast extract 5g, KH in per 1000mL distilled water2PO4 4g、KHPO4 4g、
MgSO4 1g、ZnCl23g；PH value 7.6, uses the preceding 20min that sterilized in 121 DEG C.
Step 3）The concentration of xylo-oligosaccharide and beta glucan is 2.0g/L in mixed liquor, and the concentration of trehalose is 5wt%,
The concentration of skimmed milk is 20wt%.
Performance test
Test 1：Probiotics removes ammonia nitrogen experiment
The measure of ammonia nitrogen：In the presence of sodium nitroprusside, ammonium ion can be generated with salicylate and hypochlorite ion reaction
Blue compound.Color is deeper, and explanation content is higher, and light absorption value is measured at the nm of wavelength 697 can carry out quantitative analysis.
Fig. 1 is ammonia nitrogen clearance rate change curve of the probiotics of the present invention under different time to breeding water body.From Fig. 1
In it is visible, gained probiotics reaches as high as 65% to the clearance rate of ammonia nitrogen in breeding water body.
Test 2：Probiotics removes nitrites test
The measure of nitrite nitrogen：Nitrite is the intermediate product of nitrogen cycle, unstable., can be by oxygen according to water environment conditions
Nitrite is melted into, ammonification can be also reduced.In phosphoric acid medium, when pH value is 1.8 ± 0.3, nitrite and p- aminobenzene
Sulfuryl amine reaction generate diazol, then with N-（1- naphthyls）- ethylenediamine coupling generation orchil.Have most at 540nm wavelength
It is big to absorb.
Fig. 2 is nitrite clearance rate change curve of the probiotics of the present invention under different time to breeding water body.
It can be seen in fig. 2 that gained probiotics is up to 75% to the clearance rate of breeding water body nitrite.
Test 3：Probiotics stability test
After being preserved 0 month, 1 month, 2 months, 3 months under the conditions of the gained probiotics of embodiment 1 at normal temperatures lucifuge, point
The bacillus in preparation and the content of low temperature lactobacillus Qu Yang not be detected, 1 is the results are shown in Table.
The stability test of table 1
From table 1, probiotics of the present invention has long-term stability in normal temperature environment.
Test 4：Influence to Cynoglossus semilaevis survival rate of seedling
Cynoglossus semilaevis seedling is divided into two groups, every group of 100 tails, probiotics made from the test group addition embodiment of the present invention 1
Fed, control group is fed only with conventional feed, it the results are shown in Table 2.
Experiment of the probiotics of table 2 to survival rate of seedling
From table 2, probiotics of the present invention can effectively improve the survival rate of Cynoglossus semilaevis seedling.
Test 5：Influence to Cynoglossus semilaevis growth-weight gain
Cynoglossus semilaevis is divided into two groups, every group of 40 tails, probiotics, addition made from the test group A addition embodiment of the present invention 1
The 1wt% for forage volume is measured, control group B is fed only with conventional feed；Every group do 3 it is parallel, 1,2,3 are designated as respectively, totally 6 groups,
240 tails.Raise after 8 weeks, empty stomach 24h is measured, and calculate the rate of body weight gain of Cynoglossus semilaevis, its computational methods is as follows：
,
Wherein W1、W2Respectively Cynoglossus semilaevis initial weight and end are heavy.Acquired results are as shown in table 3.
Influence experiment of the probiotics of table 3 to Cynoglossus semilaevis rate of body weight gain
From table 3, test group A average growth rate is 67.62%, hence it is evident that higher than control group B average growth rate
（55.19%）.
Above-mentioned result of the test shows, compared with conventional feed is fed, using probiotics of the present invention to Cynoglossus semilaevis
Cultivation shows preferable effect.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to the covering scope of the present invention.
Claims (6)
1. a kind of preparation method of probiotics for cynoglossus semilaevis cultivation, it is characterised in that：Comprise the following steps：
1）Bacillus and the culture of low temperature lactobacillus：Bacillus and lactobacillus strain are taken from test tube slant culture medium
Go out, be inoculated into respectively in fluid nutrient medium, be subsequently placed in shaking table, 24h is cultivated under the conditions of 20 DEG C, 150-250r/min；
2）By step 1）Bacillus bacterium solution and lactobacillus bacterium solution in logarithmic phase are inoculated into fermentation medium and carried out respectively
Fermentation, fermentation temperature is 20 DEG C, and fermentation time is 48-72h；
3）By step 2）Fermentation of bacillus liquid and lactobacillus ferment liquid in logarithmic phase are centrifuged respectively at 10000r/min
20min, with separating thallus and supernatant；Gained thalline is added in protective agent jointly and stirred and evenly mixed, then through vacuum freeze drying
The probiotics is made.
2. the preparation method of probiotics according to claim 1, it is characterised in that：Step 1）Used by middle bacillus
Liquid Culture based formulas is：5-8g containing peptone, dusty yeast 1-3g in per 1000mL distilled water；PH value 7.6-7.8；
Fluid nutrient medium used by lactobacillus is MRS culture mediums.
3. the preparation method of probiotics according to claim 1, it is characterised in that：Step 2）Middle lactobacillus bacterium solution and
The inoculum concentration of lactobacillus bacterium solution is 2vol%, and both are at fermentative medium formula used：Containing solubility in per 1000mL distilled water
Starch 3-3.5g, peptone 10-12g, yeast extract 3-5g, KH2PO4 1.5-4g、KHPO4 2-4g、MgSO4 0.1-1g、ZnCl2
1-3g；PH value 7.4-7.6.
4. the preparation method of probiotics according to claim 1, it is characterised in that：Step 3）Described in protective agent by low
Xylan, beta glucan, trehalose, skimmed milk and water composition；
Wherein, the concentration of xylo-oligosaccharide and beta glucan is 2.0g/L, and the concentration of trehalose is 5wt%, and the concentration of skimmed milk is
20wt%。
5. probiotics made from a kind of method as claimed in claim 1.
6. a kind of application of probiotics as claimed in claim 5, it is characterised in that：It is used for half sliding-tongue as feed addictive
The cultivation of sole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710335519.9A CN106957808A (en) | 2017-05-12 | 2017-05-12 | A kind of probiotics for cynoglossus semilaevis cultivation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710335519.9A CN106957808A (en) | 2017-05-12 | 2017-05-12 | A kind of probiotics for cynoglossus semilaevis cultivation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106957808A true CN106957808A (en) | 2017-07-18 |
Family
ID=59483059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710335519.9A Pending CN106957808A (en) | 2017-05-12 | 2017-05-12 | A kind of probiotics for cynoglossus semilaevis cultivation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106957808A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418924A (en) * | 2017-09-22 | 2017-12-01 | 福州大学 | A kind of preparation method and applications of polar region beneficial bacteria member |
| CN109527269A (en) * | 2018-12-06 | 2019-03-29 | 天津农学院 | It is a kind of for improving the compound of micro-ecological in intestines |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497906A (en) * | 2013-08-02 | 2014-01-08 | 国家海洋局第三海洋研究所 | Microecological preparation, preparation method, and applications thereof |
| CN105907674A (en) * | 2016-05-04 | 2016-08-31 | 福州大学 | Preparation method and application of composite microbial ecological agent |
-
2017
- 2017-05-12 CN CN201710335519.9A patent/CN106957808A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103497906A (en) * | 2013-08-02 | 2014-01-08 | 国家海洋局第三海洋研究所 | Microecological preparation, preparation method, and applications thereof |
| CN105907674A (en) * | 2016-05-04 | 2016-08-31 | 福州大学 | Preparation method and application of composite microbial ecological agent |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107418924A (en) * | 2017-09-22 | 2017-12-01 | 福州大学 | A kind of preparation method and applications of polar region beneficial bacteria member |
| CN109527269A (en) * | 2018-12-06 | 2019-03-29 | 天津农学院 | It is a kind of for improving the compound of micro-ecological in intestines |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106721260A (en) | A kind of preparation and its application of anti diar rhea type piglet specific complex probiotics | |
| CN103173371B (en) | Production of saccharomyces cerevisiae and lactobacillus acidophilus composite microbe preparation used for feed | |
| CN101273757B (en) | Lactobacillus reuteri lyophilized preparation and method for preparing the same | |
| CN110372105B (en) | Compound microbial agent for improving aquaculture water and preparation method thereof | |
| CN101974463B (en) | Lactobacillus reuteri and composite viable bacteria preparation thereof | |
| CN103468594B (en) | Candidautilis strain and application thereof | |
| CN102220262A (en) | Composite microbiological preparation and preparation method and use thereof | |
| CN103184174B (en) | Production method of bacillus subtilis biological agent used for sodium humate-containing feed in medium | |
| CN101548716A (en) | A porcine lactobacillu plantarurn freeze-dry preparation and its preparation method | |
| CN107279462A (en) | The method of chrysanthemum meal fermenting and producing aquatic products livestock and poultry active peptide powder | |
| CN106889306A (en) | A kind of feed fermentation agent and the application on feed fermentation | |
| CN102907585B (en) | A kind of middle pig probiotic vitamin premix and batch | |
| CN105132321A (en) | Enterococcus faecium, culture medium thereof for high-density solid-state fermentation, and high-density solid-state fermentation method | |
| CN102965316A (en) | Feed additive, premix and mixture containing like-bacteriocin and pediococcus acidilactici | |
| CN102919557A (en) | Growing fattening pig probiotics composite premix and accessory material | |
| CN105907674A (en) | Preparation method and application of composite microbial ecological agent | |
| CN106957808A (en) | A kind of probiotics for cynoglossus semilaevis cultivation | |
| CN106721523A (en) | A kind of probiotics for being applied to turbot cultivation | |
| CN103992977A (en) | Method for culturing bacillus subtilis through high-density fermentation | |
| CN109123076A (en) | A kind of production method of livestock and poultry vitamin B2 auxotype probiotics | |
| CN108641983A (en) | A kind of compound EM bacteria agent of multiple-effect powdery and its activation culture and application | |
| CN106858157A (en) | A kind of bacteria preparation for aquaculture | |
| CN103160453B (en) | Preparation method of microecologics for nitrite degradation, microecologics and use thereof | |
| CN1908155A (en) | Microbial viable bacteria combination and preparation method thereof | |
| CN107760612A (en) | A kind of aspergillus niger yy07 bacterial strains and its application in solid fermentation produces feeding acid protease |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170718 |