CN106954448A - Applications of the GLDA and IDS in terms of Festuca Arundinacea adverse circumstance planting - Google Patents
Applications of the GLDA and IDS in terms of Festuca Arundinacea adverse circumstance planting Download PDFInfo
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- CN106954448A CN106954448A CN201710172200.9A CN201710172200A CN106954448A CN 106954448 A CN106954448 A CN 106954448A CN 201710172200 A CN201710172200 A CN 201710172200A CN 106954448 A CN106954448 A CN 106954448A
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- glda
- ids
- festuca arundinacea
- planting
- adverse circumstance
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G20/00—Cultivation of turf, lawn or the like; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The invention discloses application of the complexing agent in terms of for Festuca Arundinacea adverse circumstance planting；Described complexing agent is GLDA or IDS, and its concentration is respectively 3 mmol kg‑1.Particularly plant improves its resistance by increasing the activity of antioxidant enzyme.The namely enhancement containing GLDA or IDS complexing agents to SOD and CAT and adjustment effect.The present invention develops it and active enhancement of Festuca Arundinacea antioxidase SOD, CAT is acted on by adding various concentrations GLDA and IDS in soil, and being applied to Festuca Arundinacea adverse circumstance planting application for GLDA and IDS provides technical support.
Description
Technical field
The invention belongs to garden afforestation technology field, it is related to GLDA and IDS answering in terms of for Festuca Arundinacea adverse circumstance planting
With.
Background technology
With the development of industrial or agricultural, environmental problem becomes increasingly conspicuous, of common occurrence the problem of heavy-metal contaminated soil.2014
April 17, Chinese Ministry of Environmental Protection and Ministry of Land and Resources's joint issue《National Soil Pollution Investigation publication》It has been shown that, China's arable land soil
Earth environmental quality causes anxiety, and point position exceeding standard rate is 19.4%, and wherein heavy metal pollution problem is than more prominent.Heavy metal in soil not
It can be decomposed by the microorganisms, and can constantly be accumulated in soil, influence soil property, it might even be possible to be converted into the bigger alkyl of toxicity
Compound, can also be absorbed by crops and other biological, be enriched with, and then influence human health by food chain.Therefore, heavy metal
The reparation of contaminated soil is generally paid close attention to by people.Soil restoring technology includes peripheral doses, chemical remediation and biological prosthetic,
Wherein, phytoremediation technology, is exactly the enrichment using green plants heavy metal, by the ground biomass for harvesting plant
Reach the purpose for removing heavy metal in soil.Due to advantages such as its is with low cost, environmental protections, repaiied in recent years as contaminated soil
Multiple field focus of attention.
The sodium of glutamic acid diacetic acid four（GLDA）, mainly it is prepared from by plant material, it is readily biodegradable, with metal ion
Sequestering power be not less than EDTA.In addition, GLDA is dissolved in after water through determining in alkalescence, when reduction pH is 4,7, it can improve
GLDA is to Soil leaching clearance.There are some researches show GLDA can remove the heavy metal in sludge, and can effectively facilitate east
Southern red-spotted stonecrop extracts the heavy metal in contaminated soil.
Iminodisuccinic acid tetrasodium salt（IDS）, it is a kind of new amino carboxylic acid quasi-chelate compound, with biodegradable
Property, there is very strong sequestering power to metal ion, especially splendid to the sequestering power of iron, copper metal ion, IDS can be used as one
Potential effectively chelating agent is planted to remove the heavy metal in battery industry sludge.Although GLDA and IDS is applied to phytoremediation weight
The research of metal has some reports, but has no that both influences to plant growth are reported.In addition, plant is by environment stress
When, plant itself has an Antioxidant Enzyme Systems to eliminate or reduce the injury that active oxygen is brought, superoxide dismutase（SOD）
And catalase（CAT）It is the Major Enzymes of this protection system, there is effect to the planting of plant adverse circumstance.But GLDA and IDS is used
In plant growth regulator, document report also there is no.
Lawn refer to perennial short herbaceous plant naturally occur or sown grassland after through relative formed by maintenance management
It is well-balanced smooth and people's leisure can be provided, travel and appropriate sports level ground shape meadow.Green grassland is to weigh modernization
One of the standard in city, is also to weigh one of standard of economic development level, the quantity and quality on lawn are anti-to a certain extent
The looks in a city are reflected.Lawn greening scale turns into the important objective mark for weighing modern city environmental quality with level
It is accurate.At present, Lawn Industry is more and more of interest for various circles of society as an industry developed rapidly.
Lawn is as the important component of urban landscaping, and the ecological benefits of its performance and the economic benefit of presence are
Gradually it is known.First, lawn is as an ecosystem, and it is a huge carbon source depots, lawn vegetation
The CO in air can be assimilated2, and lawn soil can equally inhale the CO in air2.Research shows that grassland soil is every in the world
Year can absorb deposition 0.01 ~ 0.3Gt carbon.Therefore CO in air is reduced2, mitigate greenhouse effects to a certain extent.Secondly,
Fine and close ground mulching layer and grass roots layer that lawn is formed have the effect of the good preventing and treating soil erosion.3rd, lawn tool
There is SO in absorption air2And NO2The oxygen for human breathing is discharged simultaneously etc. toxic gas.4th, lawn can slow down ground
The solar radiation of table, the heat 50% or so that the sun is mapped to ground can be absorbed by the transpiration of green plants.5th, grass
Level ground also has the effect of reduction noise.Lawn wide one piece of 20m, can weaken 2 decibels or so of noise.Finally, urban lawn is people
The product of class civilization, it can both beautify the environment, and can not provide the important work(such as the place of rest, amusement and sports for people again
Energy.
It is one of important content for must take into consideration in modern city ecological construction that turf establishment system, which is built, is modern city
Social development and a strong embodiment of economic strength, the structure of turf establishment system is for improving City Investment Environment, attracting
Foreign capitals, promote tourism etc. various aspects all play a part of can not ignore, and in city lawn greening quality and area also into
To evaluate the important objective standard of city environmental quality.Lawn greening is also increasingly subject to the attention of people.
However lawn plant is in adverse circumstance planting, because of superoxide dismutase（SOD）And catalase（CAT）'s
Synthesis is restricted, and influences the quality of turf establishment.Therefore, searching out to promote under adverse environmental factor, lawn plant（SOD）With
（CAT）The technology of synthesis should be significant.
Plant is in adverse circumstances under stress, and the generation of active oxygen is broken with the dynamic equilibrium removed in cell, and active oxygen is big
Amount is produced, and excessive active oxygen will attack the large biological molecules such as protein, nucleic acid, lipid and cause oxidative damage.SOD is in antioxygen
Change and played a significant role in system of defense, it can remove O2-Free radical, makes it be converted into H2O2, and H2O2Then by POD and CAT
Remove, generate H2O and O2。
The content of the invention
The present invention is experiment material from the common Festuca Arundinacea of northern China, by adding various concentrations GLDA in soil
And IDS, develop its to Festuca Arundinacea antioxidase SOD, CAT activity promote working technology, be GLDA and IDS be applied to Festuca Arundinacea
Adverse circumstance planting application provides technical support.
The invention discloses application of the complexing agent in terms of for Festuca Arundinacea adverse circumstance planting；Described complexing agent be GLDA or
IDS, its concentration is respectively 3 mmol kg-1。
It is of the present invention to be referred to for Festuca Arundinacea adverse circumstance planting：Festuca Arundinacea is inverse by increasing the activity of antioxidant enzyme
To improve its resistance.Described Festuca Arundinacea adverse circumstance planting is referred to：Enhancement and tune of GLDA the or IDS complexing agents to SOD and CAT
Section is acted on.
Brief description of the drawings：
Fig. 1 is that enhancements of the low concentration GLDA and IDS to Festuca Arundinacea ground biomass is acted on；
Fig. 2 is that enhancements of the low concentration GLDA and IDS to Festuca Arundinacea defence enzyme activity content is acted on.
Embodiment：
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is
Method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, to be not intended to limit the present invention model
Enclose, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, without departing substantially from this hair
On the premise of bright spirit and scope, the various changes or change carried out to the material component in these embodiments and consumption also belong to
In protection scope of the present invention.The used sodium of glutamic acid diacetic acid four（GLDA）, iminodisuccinic acid tetrasodium salt（IDS）
By commercially available.
Embodiment 1
1 develops materials and methods
1.1 materials
Experiment is derived from the topsoil of 0 ~ 20 cm in campus with soil, natural air drying, pulverizes, and sieves for subsequent use.The soil texture is sand
Matter clay, pH 7.44, full potassium 45.61%, full nitrogen 0.21%, the mg kg of available phosphorus 22.03-1, the % of the content of organic matter 4.68, saturation
Water content 56%, the g cm of unit weight 0.87-3.Lawn plant chooses the common Festuca Arundinacea of northern China（Festuca arundinacea
Schreb.）.IDS is purchased from County, Hebei Province, China Shijiazhuang Development Zone Desai Chemical Co., Ltd, the g cm of density 1.32 ~ 1.35-3,
PH 10.3-11.4, effective content is 75%；GLDA is purchased from Akzo Nobel（Akzo Nobel）Chemical Co., Ltd., pH
10-11, effective content is 47.4%.
1.2 lawn plants are cultivated
The tall fescue seed of full seed is selected, is soaked 24 hours under dark condition in advance.Choose a diameter of 6.8 cm, it is a height of
7.8 cm plastic tub, loads 150g soil, and 100 seeds are sowed per basin.Complexing agent is after planting added, 5 are set respectively not
With concentration processing：3rd, 6,9,12 and 15 mmol kg-1, using the processing without complexing agent as control（Control）, each processing
3 repetitions.18~25 DEG C of indoor temperature between plant culture period, relative humidity is 35 ~ 65%, and illumination is the nature for penetrating interior
Light（6856~27090 LX）.Keep the skin wet in right amount daily, to ensure plant normal growth.
1.3 methods of production
1.3.1 phytomass is determined
Plant seeding is cradled after 30 days, and finish 20 min at 108 DEG C of aerial part, 80 DEG C of drying to constant weight.Ground
1.3.2 the measure of enzyme
Crude enzyme liquid is extracted：It is accurate to weigh the g of fresh grass sample 0.5, add the ml of precooling 3 extract solution（50 mmol/ L phosphoric acid buffer
Liquid pH7.0, includes 1 mmol/ L EDTA）With a little quartz sand, it is fully ground under condition of ice bath, is transferred in centrifuge tube, then with 2
Ml extract solutions wash mortar, merge extract solution and simultaneously centrifuge 20 min in 10000 r/min at 4 DEG C, supernatant in 4 DEG C of Refrigerator stores,
For enzyme activity assay.
CAT determination of activity ultraviolet spectrophotometries, CAT enzyme-activity unit（U）It is defined as：Every gram of fresh weight is per minute to be made
OD470And OD240The enzyme amount of increase by 0.1 is an enzyme-activity unit（U·g-1·min-1）.The measure chlorination nitro of SOD activity
Tetrazole is blue（NBT）Photochemical reduction method, it is a unit of enzyme activity to suppress NBT photochemical reductions 50% with every gram of fresh weight（U ·
g-1）.
1.3 data processings
Data carry out one-way analysis of variance using SPSS 19.0（One-way ANOVA）, and Tukey methods are used,P=
0.05 level carries out data difference significance test, is drawn a diagram using Microsoft Excel 2007.
2 development results are analyzed
Enhancements of the 2.1 low concentration GLDA and IDS to biomass is acted on
Add influence of the complexing agent of various concentrations to Festuca Arundinacea dry weight above and below the ground as shown in Figure 1.As can be seen that low concentration
Accumulation of the complexing agent to Festuca Arundinacea ground biomass have certain facilitation, complexing agent concentration is 6 mmol kg-1Place
Reason, ground dry weight reaches maximum, notable with contrast difference.Complexing agent concentration is higher than 6 mmol kg-1Processing.
Enhancements of the GLDA and IDS of 2.2 low concentrations to Festuca Arundinacea defence enzyme activity is acted on
CAT and SOD activity shows first to increase the trend reduced afterwards with the increase of two kinds of complexing agent concentrations.CAT activity is high respectively
Go out control 5.18% and 10.41%（P ＜0.05）, but SOD activity is not notable with contrast difference.When complexing agent concentration is more than 3 mmol
kg-1, CAT and SOD activity are reduced with the increase of complexing agent concentration.
3 develop conclusion：
Add complexing agent and environment stress is constituted to Festuca Arundinacea, cause Lipid peroxidation metabolism, plant is by increasing the work of antioxidant enzyme
Property improves its resistance.In the present invention, using Festuca Arundinacea as object, optimization is developed in soil and adds various concentrations（0、3、6、
9th, 12 and 15 mmol kg-1）The sodium of biodegradable complexing agent glutamic acid diacetic acid four（GLDA）With the sodium of iminodisuccinic acid four
Salt（IDS）Working technology is promoted to SOD and CAT.As a result show, compared with control, the complexing agent of low concentration（3 mmol kg-1）
Have to SOD and CAT and promote and adjustment effect.This improves its resistance for Festuca Arundinacea by increasing the activity of antioxidase
Greening planting has significant application value.
Claims (3)
1. application of the complexing agent in terms of for Festuca Arundinacea adverse circumstance planting；Described complexing agent is GLDA or IDS, and its concentration is respectively
3 mmol kg-1。
2. the application described in claim 1, wherein being referred to for Festuca Arundinacea adverse circumstance planting：Festuca Arundinacea is inverse anti-oxidant by increasing
The activity of thing enzyme improves its resistance.
3. the application described in claim 1, wherein being referred to for Festuca Arundinacea adverse circumstance planting：GLDA or IDS complexing agents to SOD and
CAT enhancement and adjustment effect.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710172200.9A CN106954448A (en) | 2017-04-20 | 2017-04-20 | Applications of the GLDA and IDS in terms of Festuca Arundinacea adverse circumstance planting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710172200.9A CN106954448A (en) | 2017-04-20 | 2017-04-20 | Applications of the GLDA and IDS in terms of Festuca Arundinacea adverse circumstance planting |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111250535A (en) * | 2020-02-18 | 2020-06-09 | 四川省蜀通岩土工程公司 | Heavy metal composite contaminated site soil remediation method |
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| CN111250535A (en) * | 2020-02-18 | 2020-06-09 | 四川省蜀通岩土工程公司 | Heavy metal composite contaminated site soil remediation method |
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Application publication date: 20170718 |
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