CN106942574A - A kind of preparation method of Mytilus galloprovincialis edible melanin - Google Patents

A kind of preparation method of Mytilus galloprovincialis edible melanin Download PDF

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Publication number
CN106942574A
CN106942574A CN201710218425.3A CN201710218425A CN106942574A CN 106942574 A CN106942574 A CN 106942574A CN 201710218425 A CN201710218425 A CN 201710218425A CN 106942574 A CN106942574 A CN 106942574A
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activated carbon
mytilus galloprovincialis
centrifugation
weight
filter liquor
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CN201710218425.3A
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Chinese (zh)
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熊廷珍
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Individual
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Meat, Egg Or Seafood Products (AREA)

Abstract

The present invention relates to a kind of preparation method of Mytilus galloprovincialis edible melanin, comprising production stage have:Pretreatment of raw material, broken, enzymolysis, filtering, attached Adsorption and desorption, centrifugation, washing, second of centrifugation, dry.The method working condition is gentle, controllable, and technological process is simple, repeats and utilizes activated carbon and NaOH solution, realizes sustainable use.The Mytilus galloprovincialis edible melanin extraction rate reached 0.9 1.5% prepared, contain protein 33.3 38.5%, polysaccharide 31.9 41.7%, with higher feature and trophism in extract, it is the preferred product for substituting synthesis edible melanin, while also increasing the economic worth of Mytilus galloprovincialis.

Description

A kind of preparation method of Mytilus galloprovincialis edible melanin
Technical field
The present invention relates to field of food, more particularly to a kind of preparation method of Mytilus galloprovincialis edible melanin.
Background technology
Food coloring is to make food color or improve the food additives of food tone and color and luster, in recent years, with safety The development of toxicology and analysis and testing technology, has progressively confirmed that partially synthetic pigment has certain harm, some conjunctions to health Into pigment even can lead to diarrhea, teratogenesis, slow poisoning and carcinogenic, with the increase of the food-safe problem attention degree of people, Consumer increasingly worried the edible safety of synthetic dyestuff, therefore, both at home and abroad current all opening research steering natural pigment Hair.Natural black pigment is divided into animal class, plant and microbiology class melanin, with deepening continuously for studying it, finds black Pigment has extensive bioactivity, including anti-oxidant, removing free radical, regulation immunity, protection light injury, the cardiovascular work(of protection It can wait, all be had broad application prospects in terms of food processing, cosmetics exploitation, prophylactic treatment.
Mytilus galloprovincialis(Mytilus edulis Linnaeus)Marine rainbow is commonly called as, belongs to sea mollusk, is high latitude inner bay Shellfish, it is coastal to be distributed widely in China Liaoning, Shandong, Zhejiang etc., with strong adaptability, flourish be fast, yield is high and nutrition Abundant the features such as, the title of " ocean egg " is have, the traditional Chinese medical science thinks that it has the effects such as nourishing yin and supplementing blood, strengthening the essence kidney tonifying.Hao Shixin etc. Research is found, contains melanin in Mytilus galloprovincialis shell.Outer embrane is that mollusk secretes the organ to form shell, therefore in shell Contained melanin comes from outer embrane.China's Mytilus galloprovincialis annual production is extracted melanin up to 500,000 tons, for outer embrane and provided Substantial amounts of material base, if a kind of new edible melanin can be developed, will be greatly improved the added value of Mytilus galloprovincialis, increases fisherman Income.
At present, the extraction for having been reported that and melanin being carried out from Mytilus galloprovincialis outer embrane was not met, and the present invention still belongs to the first time.
The content of the invention
The invention aims to develop novel edible melanin, Mytilus galloprovincialis added value is improved.
The purpose of the present invention reaches as follows:
a)Pretreatment of raw material:Fresh Mytilus galloprovincialis is subjected to cleaning silt, 90 DEG C of blanching 5-10min cool to room temperature, take out mussel Meat, mussel meat main body is peeled off by outer embrane black border at mussel opening, collects black overcoat film edge thing;
b)It is broken:By step a)The overcoat film edge thing of acquisition adds mixer and rubbed;
c)Enzymolysis:To step b)In fragment in, add 5-10 times of weight purified water, add gross weight 0.1-3% protease Powder, adjusts pH value, temperature, enzymolysis is extremely without solid content, enzyme activity of going out to specifications to specifications;
d)Filtering:Enzymolysis liquid is filtered using nylon leaching net, insoluble tissue residue is removed, retains filter liquor;
e)Absorption:Filter liquor is adsorbed using the activated carbon of filter liquor 1/10-1/5 weight, number is stood after addition activated carbon Hour, until filter liquor clarification, outwells clarification filter liquor, retain the activated carbon after absorption;
f)Desorption:The 0.2-2mol/L of 2-5 times of weight of raw material food-grade NaOH, stirring are added into the activated carbon after absorption Activated carbon is removed after 30-60min, nylon net filter, adsorption liquid is obtained, repeats and utilize after activated carbon washing;
g)Centrifugation:Adsorption liquid is centrifuged, rotating speed 4500r/min, centrifuge 1h, pour out supernatant to recycle, retain heavy Form sediment;
h)Washing:The purified water of the weight such as raw material is added into precipitation, is dissolved, obtains water lotion;
i)Second of centrifugation:Water lotion is centrifuged, rotating speed 4500r/min, centrifuge 1h, outwell supernatant, retain precipitation;
j)Dry:By step i)The precipitation of middle acquisition is put into baking oven, and 80 DEG C of dryings produce Mytilus galloprovincialis and eat black to moisture < 3% Element.
It is preferred that, protease in step c include one kind in compound protease, flavor protease, neutral proteinase or It is several.
Compared with prior art, it is an advantage of the invention that:Mytilus galloprovincialis edible melanin production process mild condition is controllable, work Skill simple flow, the NaOH solution and activated carbon used is reusable, realizes recycling for resource.The purple of acquisition is made a gift of Shellfish edible melanin recovery rate is 0.9-1.5%, 33.3-38.5% containing protein, polysaccharide 31.9-41.7% in extract, with compared with High feature and trophism, is the preferred product of edible melanin of new generation.
Brief description of the drawings
Fig. 1 is the technological process of production of the present invention.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Embodiment 1
The fresh Mytilus galloprovincialis of 100kg is bought in market, is rinsed using giant, boiling pond sets 90 DEG C of water temperature, blanching 10min, pulls placement out to room temperature, clam meat is taken out by hand, opening outer embrane black border is separated from main body, is weighed after collection For 5.7kg, mixer adds purified water 50kg after rubbing, and adds 300g compound protein enzyme powders, 55 DEG C of insulation 24h, 80 DEG C of insulations 10min, obtains enzymolysis liquid.Enzymolysis liquid is filtered using the nylon wire of 100 mesh, retains filtered fluid, gradually adds and amounts to 10kg Activated carbon powder, stands after 2h, filters to obtain absorption activated carbon.It is that 1mol/L eats NaOH that 15kg concentration is added into activated carbon, is stirred Mix 30min carry out desorption, using 100 mesh nylon net filters, recovered carbon is rinsed with purified water, continuation filtrate is carried out from The heart, rotating speed 4500r/min centrifugation 1h, reclaims supernatant NaOH solution, and 5.7kg purified waters are added into centrifugation and are washed Centrifugation, rotating speed 4500r/min centrifugation 1h, abandoning supernatant retains precipitation, puts to 80 DEG C of dry 24h of baking oven, use water analysis Instrument is detected that moisture is 2.85%, produces Mytilus galloprovincialis edible melanin 1.3kg.Measuring protein content using Kjeldahl's method is 35.1%, it is 32.7% to measure polyoses content using anthrone-sulfuric acid process.
Embodiment 2
The fresh Mytilus galloprovincialis of 200kg is bought in market, is rinsed using giant, boiling pond sets 90 DEG C of water temperature, blanching 10min, pulls placement out to room temperature, clam meat is taken out by hand, opening outer embrane black border is separated from main body, is weighed after collection For 11.5kg, mixer adds purified water 95kg after rubbing, and adds 560g neutral protein enzyme powders, 55 DEG C of insulation 24h, 80 DEG C of insulations 10min, obtains enzymolysis liquid.Enzymolysis liquid is filtered using the nylon wire of 80 mesh, retains filtered fluid, gradually adds and amounts to 20kg work Property powdered carbon, stand 2h after, filter to obtain absorption activated carbon.It is that 1mol/L eats NaOH, stirring that 25kg concentration is added into activated carbon 30min carry out desorption, using 100 mesh nylon net filters, recovered carbon is rinsed with purified water, continuation filtrate is carried out from The heart, rotating speed 4500r/min centrifugation 1h, reclaims supernatant NaOH solution, and 11.5kg purified waters are added into centrifugation and are washed Centrifugation is washed, rotating speed 4500r/min centrifugation 1h, abandoning supernatant retains precipitation, put to 80 DEG C of dry 24h of baking oven, uses moisture point Analyzer is detected that moisture is 2.90%, produces Mytilus galloprovincialis edible melanin 2.5kg.Protein content is measured using Kjeldahl's method For 33.7%, it is 37.5% to measure polyoses content using anthrone-sulfuric acid process.

Claims (2)

1. a kind of preparation method of Mytilus galloprovincialis edible melanin is made by following steps:
a)Pretreatment of raw material:Fresh Mytilus galloprovincialis is subjected to cleaning silt, 90 DEG C of blanching 5-10min cool to room temperature, take out mussel Meat, mussel meat main body is peeled off by outer embrane black border at mussel opening, collects black overcoat film edge thing;
b)It is broken:By step a)The overcoat film edge thing of acquisition adds mixer and rubbed;
c)Enzymolysis:To step b)In fragment in, add 5-10 times of weight purified water, add gross weight 0.1-3% protease Powder, adjusts pH value, temperature, enzymolysis is extremely without solid content, enzyme activity of going out to specifications to specifications;
d)Filtering:Enzymolysis liquid is filtered using nylon leaching net, insoluble tissue residue is removed, retains filter liquor;
e)Absorption:Filter liquor is adsorbed using the activated carbon of filter liquor 1/10-1/5 weight, number is stood after addition activated carbon Hour, until filter liquor clarification, outwells clarification filter liquor, retain the activated carbon after absorption;
f)Desorption:The 0.2-2mol/L of 2-5 times of weight of raw material food-grade NaOH, stirring are added into the activated carbon after absorption Activated carbon is removed after 30-60min, nylon net filter, adsorption liquid is obtained, repeats and utilize after activated carbon washing;
g)Centrifugation:Adsorption liquid is centrifuged, rotating speed 4500r/min, centrifuge 1h, pour out supernatant to recycle, retain heavy Form sediment;
h)Washing:The purified water of the weight such as raw material is added into precipitation, is dissolved, obtains water lotion;
i)Second of centrifugation:Water lotion is centrifuged, rotating speed 4500r/min, centrifuge 1h, outwell supernatant, retain precipitation;
j)Dry:By step i)The precipitation of middle acquisition is put into baking oven, and 80 DEG C of dryings produce Mytilus galloprovincialis and eat black to moisture < 3% Element.
2. a kind of preparation method of Mytilus galloprovincialis edible melanin according to claim 1, it is characterised in that described step Protease in c includes the one or more in compound protease, flavor protease, neutral proteinase.
CN201710218425.3A 2017-04-05 2017-04-05 A kind of preparation method of Mytilus galloprovincialis edible melanin Pending CN106942574A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107821924A (en) * 2017-11-14 2018-03-23 蚌埠市涂山村富民石榴专业合作社 A kind of extraction process of garnet pigment
CN108236600A (en) * 2018-01-02 2018-07-03 刘莹艳 A kind of suncream
CN108997184A (en) * 2018-08-10 2018-12-14 浙江海洋大学 A kind of high efficiency extraction and purification process of mussel carotenoid

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
于文超,等: "长牡蛎(Crassostrea gigas)贝壳与外套膜中黑色素的提取和鉴定", 《海洋与湖沼》 *
刘惠宾,等: "活性炭对鱼蛋白水解", 《北京水产》 *
郝世鑫,等: "紫贻贝贝壳和脉红螺厣中色素的提取与鉴定", 《海洋科学》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107821924A (en) * 2017-11-14 2018-03-23 蚌埠市涂山村富民石榴专业合作社 A kind of extraction process of garnet pigment
CN108236600A (en) * 2018-01-02 2018-07-03 刘莹艳 A kind of suncream
CN108997184A (en) * 2018-08-10 2018-12-14 浙江海洋大学 A kind of high efficiency extraction and purification process of mussel carotenoid
CN108997184B (en) * 2018-08-10 2020-04-28 浙江海洋大学 Efficient extraction and purification method of mussel carotenoid

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Application publication date: 20170714