CN106942066A - A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem - Google Patents
A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem Download PDFInfo
- Publication number
- CN106942066A CN106942066A CN201710334159.0A CN201710334159A CN106942066A CN 106942066 A CN106942066 A CN 106942066A CN 201710334159 A CN201710334159 A CN 201710334159A CN 106942066 A CN106942066 A CN 106942066A
- Authority
- CN
- China
- Prior art keywords
- culture
- explant
- treebine
- rapid propagation
- treebine stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to the method for Plant Tissue Breeding in agricultural biotechnologies, specifically, it is related to a kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem, including the following steps carried out successively：The explant of acquisition treebine stem plant, explant Fiber differentiation, Multiplying culture, culture of rootage, transplanting；The explant inducing culture of described explant Fiber differentiation operation includes：MS culture mediums, the BA of 0.5~2.0mg/L 6,0.2~0.5mg/L NAA, 0.01~0.05mg/L Paclobutrazol, 25~30g/L sucrose, 3.5~4.0g/L agar, 0.5~1.0g/L activated carbons, pH is 5.4~5.8, present invention selection treebine stem stem-segment with node is explant, through Fiber differentiation, Multiplying culture, culture of rootage and transplanting and other steps, its inductivity has reached more than 88.5%, survival rate is to more than 95%, the tissue-culturing rapid propagation of treebine stem is realized, it is achieved thereby that the purpose of the present invention.
Description
Technical field
The present invention relates to the method for Plant Tissue Breeding in agricultural biotechnologies, specifically, it is related to a kind of Guangxi characteristic
The tissue culture and rapid propagation method of Yao nationality's medicine treebine stem.
Background technology
Treebine stem (Cissus Pteroclada Hayata), the red treebine stem of alias, wing branch Cissus repens, spring root rattan etc., it is
Vitaceae (Vitaceae) Cissus (Cissus Linn.) herbaceous species plant, is often born in broad-leaf forest, is Guangxi characteristic
One of Yao nationality's medicine, is distributed mainly on the ground such as Baise, Luchuan, He Prefecture, Bobai, Jingxi, it is among the people be widely used in treatment arthralgia pain due to rheumatism,
Lumbar muscle strain, wound, hemostasis etc., it is evident in efficacy.According to《Guangxi Chinese medicinal herbal》Record, cubic rattan can be used as medicine, and its mildly bitter flavor is puckery,
It is sweet, it is mild-natured.With stimulating the circulation of the blood and cause the muscles and joints to relax, stasis eliminatings life is new, controls the effects such as traumatic injury, grain contraction.Available for treatment arthralgia pain due to rheumatism, waist
The disease such as muscular strain, traumatic injury, interior traumatism and bleeding, stomach and duodenal hemorrhage, pain, and available for postpartum health care dipping.
As a kind of Yao nationality's medicine with unique drug effect, wild state is in current treebine stem more, but because medicinal herb grower is unordered
Excavation, its wild resource falls sharply, therefore artificial cultivation treebine stem seems very urgent！Treebine stem can pass through seed, cuttage, press strip
Bred etc. mode, but there is cycle length, the shortcomings of breeding coefficient is low, limit the production of treebine stem seedling.Therefore having must
The tissue culture rapid propagation system of treebine stem is set up, present invention selection treebine stem stem-segment with node is explant, through inducing, breeding, take root,
Hardening and transplanting and other steps, establish the quick breeding by group culture system of treebine stem.The present invention have the cycle it is short, with low cost,
The features such as breeding coefficient is high, can be directly used for the factorial praluction of treebine stem seedling, the plant for promoting Yao nationality's medicine medicine treebine stem
Thing development of resources has important practical significance.
To the report that so far, at home and abroad there is no treebine stem tissue culture technique, there is not treebine stem tissue-culturing rapid propagation yet
The application of patent.
The content of the invention
It is an object of the invention to provide a kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem, present invention selection four
Square rattan stem-segment with node is explant, and through Fiber differentiation, Multiplying culture, culture of rootage and transplanting and other steps, its inductivity reaches
More than 88.5%, survival rate to more than 95% realizes the tissue-culturing rapid propagation of treebine stem, it is achieved thereby that the mesh of the present invention
's.
The technical scheme that the present invention is provided is：A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem, including according to
The following steps of secondary progress：The explant of acquisition treebine stem plant, explant Fiber differentiation, Multiplying culture, culture of rootage, shifting
Plant；
The explant inducing culture of described explant Fiber differentiation operation includes：MS culture mediums, 0.5~2.0mg/L
6-BA, 0.2~0.5mg/LNAA, 0.01~0.05mg/L Paclobutrazol, 25~30g/L sucrose, 3.5~4.0g/L fine jades
Fat, 0.5~1.0g/L activated carbons, pH are 5.4~5.8.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, described explant Fiber differentiation operation
Method be：2.0~2.5cm belt segment rattan will be cut into after the treebine stem sterilization as explant collected and be inoculated with
Into inducing culture, being placed in 25~28 DEG C of full light cultures of progress 25~35 days can induced synthesis adventitious bud.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, described Multiplying culture operation is used
Proliferated culture medium include：MS culture mediums, 1.0~2.0mg/L 6-BA, 0.5~1.0mg/LNAA, 20~30g/L sucrose, 3.5
~4.0g/L agar, 0.1~0.5g/L activated carbons, pH are 5.4~5.8.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, described Multiplying culture operation is specific
For：Obtained adventitious bud is operated to be cut into long 1.5~2.0cm stem section and be inoculated into proliferated culture medium explant Fiber differentiation
The propagation of adventitious bud can be achieved for 20~30 days in culture.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, described culture of rootage operation is used
Root media include：1/2MS culture mediums, 0.05~0.1mg/L chlormequat chloride, 0.5~1.0mg/L
IBA, 15~20g/L sucrose, 3.0~4.0g/L agar, 0.1~0.3g/L activated carbons, pH are 5.4~5.8.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, the method that described culture of rootage is operated
For：From Multiplying culture bred after the base portion of adventitious bud cut 2.0~3.0cm adventitious buds and be inoculated into root media
Culture can realize taking root for test tube seedling in 25~30 days.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, the specific method that described transplanting is operated
For：Through culture of rootage the good test tube seedling of obtained root growth will be operated in the natural light lower refining seedling 2~3 days in greenhouse, cleaned
It is 1 that the culture medium of root, which is transplanted in volume ratio,:Cultivation seedling produces treebine stem seedling in 1 fertile soil, peat soil mixed-matrix.
In the tissue culture and rapid propagation method of above-mentioned Guangxi characteristic Yao nationality medicine treebine stem, Multiplying culture operation, culture of rootage behaviour
Middle condition of culture is：Cultivation temperature is 25~28 DEG C, and intensity of illumination is 2500~3000lx, and light application time is 12~14 small
When/day.
Beneficial effect：
The present invention establishes the tissue-culturing rapid propagation of Yao nationality's medicine treebine stem using plant tissue culture technique, and the present invention has the cycle
It is short, with low cost, the features such as breeding coefficient is high, the factorial praluction of treebine stem seedling is can be directly used for, for promoting Yao nationality's medicine
The plant resource explorationses of treebine stem have important practical significance.
Embodiment
With reference to embodiment, technical scheme is described in further detail, but not constituted pair
Any limitation of the present invention.
Embodiment one
(1) explant is gathered：Robust growth, the treebine stem plant middle and upper part without obvious disease are chosen in the wild, filled on the sunny side
Real belt segment rattan is explant, carries out water conservation moisturizing processing immediately after collection and takes back laboratory in time.
(2) explant is induced：The explant that step (1) is gathered back into laboratory is placed under running water and rinsed 3 hours, is placed in
Sterilize 10 seconds, used after aseptic water washing 3 times after aseptic filter paper suck dry moisture in 75% ethanol solution in superclean bench,
Be placed in 0.1% mercuric chloride solution and sterilize 15 minutes, after aseptic water washing 5 times with being cut into 2.0 after aseptic filter paper suck dry moisture~
2.5cm or so belt segment rattan is simultaneously inoculated into inducing culture, and being placed in 25 DEG C of full light cultures of progress 25 days can induced synthesis
Adventitious bud, inductivity is up to 94.1%, and pollution rate is less than 10%.Described inducing culture is：MS culture medium+2.0mg/L 6-BA
+ 0.5mg/LNAA+0.05mg/L Paclobutrazol (paclobutrazol)+25g/L sucrose+3.5g/L agar+0.5g/L activated carbons,
PH is 5.4.
(3) Multiplying culture：The adventitious bud that step (2) induction is obtained, which is cut into, to be about 1.5~2.0cm stem section and is inoculated into
The propagation that adventitious bud can be achieved for 20 days is cultivated in proliferated culture medium, growth coefficient reaches 5.0 times.Described proliferated culture medium
For：MS culture medium+2.0mg/L 6-BA+1.0mg/LNAA+20g/L sucrose+3.5g/L agar+0.1g/L activated carbons, pH is
5.4。
(4) culture of rootage：Step (3) the obtained adventitious bud for being about 2.0~3.0cm of propagation is cut from base portion and is inoculated into
Culture can realize taking root for test tube seedling in 20 days in root media, and rooting rate reaches 95.8%.Described root media is：
1/2MS culture medium+0.1mg/L chlormequat chloride (cycocel)+1.0mg/L IBA+15g/L sucrose+3.0g/L
Agar+0.1g/L activated carbons, pH is 5.4.
(5) transplant：By the good test tube seedling of step (4) root growth in the natural light lower refining seedling 2 days in greenhouse, root is cleaned
Culture medium transplant in volume ratio be 1:Cultivation seedling produces treebine stem seedling in 1 fertile soil, peat soil mixed-matrix, transplants
Survival rate is to more than 95% after 30 days.
The condition of culture of above-mentioned steps (3)~(4) is：Cultivation temperature is 25 DEG C, and intensity of illumination is 2500lx, light application time
For 12 hours/day.
Embodiment two
(1) explant is gathered：Robust growth, the treebine stem plant middle and upper part without obvious disease are chosen in the wild, filled on the sunny side
Real belt segment rattan is explant, carries out water conservation moisturizing processing immediately after collection and takes back laboratory in time.
(2) explant is induced：The explant that step (1) is gathered back into laboratory is placed under running water and rinsed 4 hours, is placed in
Sterilize 13 seconds, used after aseptic water washing 4 times after aseptic filter paper suck dry moisture in 75% ethanol solution in superclean bench,
Be placed in 0.1% mercuric chloride solution and sterilize 17 minutes, after aseptic water washing 6 times with being cut into 2.0 after aseptic filter paper suck dry moisture~
2.5cm or so belt segment rattan is simultaneously inoculated into inducing culture, and being placed in 26 DEG C of full light cultures of progress 31 days can induced synthesis
Adventitious bud, inductivity reaches more than 90%, and pollution rate is less than 15%.Described inducing culture is：MS culture mediums+1.5mg/L
6-BA+0.35mg/L NAA+0.03mg/L Paclobutrazol (paclobutrazol)+28g/L sucrose+3.7g/L agar+0.8g/L
Activated carbon, pH is 5.6.
(3) Multiplying culture：The adventitious bud that step (2) induction is obtained, which is cut into, to be about 1.5~2.0cm stem section and is inoculated into
The propagation that adventitious bud can be achieved for 25 days is cultivated in proliferated culture medium, growth coefficient reaches 3.9 times.Described proliferated culture medium
For：MS culture medium+1.5mg/L 6-BA+0.75mg/L NAA+25g/L sucrose+3.7g/L agar+0.3g/L activated carbons, pH is
5.6。
(4) culture of rootage：Step (3) the obtained adventitious bud for being about 2.0~3.0cm of propagation is cut from base portion and is inoculated into
Culture can realize taking root for test tube seedling in 28 days in root media, and rooting rate reaches more than 93%.Described root media
For：1/2MS culture medium+0.07mg/L chlormequat chloride (cycocel)+0.7mg/L IBA+18g/L sucrose+
3.6g/L agar+0.2g/L activated carbons, pH is 5.6.
(5) transplant：By the good test tube seedling of step (4) root growth in the natural light lower refining seedling 3 days in greenhouse, root is cleaned
Culture medium transplant in volume ratio be 1:Cultivation seedling produces treebine stem seedling in 1 fertile soil, peat soil mixed-matrix, transplants
30 days survival rates are to more than 96%.
The condition of culture of above-mentioned steps (3)~(4) is：Cultivation temperature is 27 DEG C, and intensity of illumination is 2500~3000lx, light
It is 13 hours/day according to the time.
Embodiment three
(1) explant is gathered：Robust growth, the treebine stem plant middle and upper part without obvious disease are chosen in the wild, filled on the sunny side
Real belt segment rattan is explant, carries out water conservation moisturizing processing immediately after collection and takes back laboratory in time.
(2) explant is induced：The explant that step (1) is gathered back into laboratory is placed under running water and rinsed 5 hours, is placed in
Sterilize 15 seconds, used after aseptic water washing 5 times after aseptic filter paper suck dry moisture in 75% ethanol solution in superclean bench,
Be placed in 0.1% mercuric chloride solution and sterilize 20 minutes, after aseptic water washing 7 times with being cut into 2.0 after aseptic filter paper suck dry moisture~
2.5cm or so belt segment rattan is simultaneously inoculated into inducing culture, and being placed in 28 DEG C of full light cultures of progress 35 days can induced synthesis
Adventitious bud, induction reaches 88.5%, and pollution rate is less than 8%.Described inducing culture is：MS culture medium+0.5mg/L 6-BA+
0.2mg/L NAA+0.01mg/L Paclobutrazol (paclobutrazol)+30g/L sucrose+4.0g/L agar+1.0g/L activated carbons,
PH is 5.8.
(3) Multiplying culture：The adventitious bud that step (2) induction is obtained, which is cut into, to be about 1.5~2.0cm stem section and is inoculated into
The propagation that adventitious bud can be achieved for 20~30 days is cultivated in proliferated culture medium, growth coefficient reaches 4.3 times.Described Multiplying culture
Base is：MS culture medium+1.0mg/L 6-BA+0.5mg/L NAA+30g/L sucrose+4.0g/L agar+0.5g/L activated carbons, pH is
5.8。
(4) culture of rootage：Step (3) the obtained adventitious bud for being about 2.0~3.0cm of propagation is cut from base portion and is inoculated into
Culture can realize taking root for test tube seedling in 35 days in root media, take root and reach more than 90%.Described root media is：
1/2MS culture medium+0.05mg/L chlormequat chloride (cycocel)+0.5mg/L IBA+20g/L sucrose+4.0g/
L agar+0.3g/L activated carbons, pH is 5.8.
(5) transplant：By the good test tube seedling of step (4) root growth in the natural light lower refining seedling 2 days in greenhouse, root is cleaned
Culture medium transplant in volume ratio be 1:Cultivation seedling produces treebine stem seedling in 1 fertile soil, peat soil mixed-matrix, transplants
Survival rate 100% after 30 days.
The condition of culture of above-mentioned steps (3)~(4) is：Cultivation temperature is 28 DEG C, and intensity of illumination is 3000lx, light application time
For 14 hours/day.
Above-described embodiment is preferably embodiment, but embodiments of the present invention are not by above-described embodiment of the invention
Limitation, other any Spirit Essences without departing from the present invention and the change made under principle, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Claims (8)
1. a kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem, it is characterised in that：Including the following step carried out successively
Suddenly：The explant of acquisition treebine stem plant, explant Fiber differentiation, Multiplying culture, culture of rootage, transplanting；
The explant inducing culture of described explant Fiber differentiation operation includes：MS culture mediums, 0.5~2.0mg/L6-BA,
0.2~0.5mg/L NAA, 0.01~0.05mg/L Paclobutrazol, 25~30g/L sucrose, 3.5~4.0g/L agar,
0.5~1.0g/L activated carbons, pH is 5.4~5.8.
2. the tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem according to claim 1, it is characterised in that：Described
Explant Fiber differentiation operation method be：2.0 are cut into after the treebine stem sterilization as explant that will be collected~
2.5cm belt segment rattan is simultaneously inoculated into inducing culture, and be placed in 25~28 DEG C of full light cultures of progress can induce for 25~35 days
Form adventitious bud.
3. the tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem according to claim 1, it is characterised in that：Described
The used proliferated culture medium of Multiplying culture operation includes：MS culture mediums, 1.0~2.0mg/L 6-BA, 0.5~1.0mg/
LNAA, 20~30g/L sucrose, 3.5~4.0g/L agar, 0.1~0.5g/L activated carbons, pH are 5.4~5.8.
4. the tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem according to claim 3, it is characterised in that：Described
Multiplying culture is operated：Obtained adventitious bud is operated to be cut into long 1.5~2.0cm stem section and connect explant Fiber differentiation
Plant the propagation cultivated into proliferated culture medium and adventitious bud can be achieved for 20~30 days.
5. the tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem according to claim 1, it is characterised in that：Described
The used root media of culture of rootage operation includes：1/2MS culture mediums, 0.05~0.1mg/L
Chlormequatchloride, 0.5~1.0mg/L IBA, 15~20g/L sucrose, 3.0~4.0g/L agar, 0.1~0.3g/
L activated carbons, pH is 5.4~5.8.
6. the tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem according to claim 5, it is characterised in that：Described
Culture of rootage operation method be：From Multiplying culture bred after the base portion of adventitious bud cut 2.0~3.0cm adventitious buds simultaneously
Taking root for test tube seedling can be realized in 25~30 days by being inoculated into culture in root media.
7. the tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem according to claim 1, it is characterised in that：Described
Transplanting the specific method operated is：The good test tube seedling of obtained root growth will be operated in the natural light in greenhouse through culture of rootage
Lower refining seedling 2~3 days, it is 1 that the culture medium of clean root, which is transplanted in volume ratio,:Cultivated into 1 fertile soil, peat soil mixed-matrix
Seedling produces treebine stem seedling.
8. according to the tissue culture and rapid propagation method of any described Guangxi characteristic Yao nationality medicine treebine stems of claim 1-7, it is characterised in that：
Condition of culture is in Multiplying culture operation, culture of rootage behaviour：Cultivation temperature be 25~28 DEG C, intensity of illumination be 2500~
3000lx, light application time is 12~14 hours/day.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710334159.0A CN106942066B (en) | 2017-05-12 | 2017-05-12 | A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710334159.0A CN106942066B (en) | 2017-05-12 | 2017-05-12 | A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106942066A true CN106942066A (en) | 2017-07-14 |
| CN106942066B CN106942066B (en) | 2018-11-27 |
Family
ID=59478655
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710334159.0A Expired - Fee Related CN106942066B (en) | 2017-05-12 | 2017-05-12 | A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106942066B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182793A (en) * | 2017-07-25 | 2017-09-22 | 广西壮族自治区中国科学院广西植物研究所 | A kind of tissue culture and rapid propagation method of precious jade anther-wings stem Cissus repens |
| CN115005096A (en) * | 2022-05-26 | 2022-09-06 | 桂林医学院 | Ethnic medicine alangium platanifolium seedling breeding method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891613A (en) * | 2014-03-24 | 2014-07-02 | 湖北民族学院 | Rapid propagation method for rejuvenation of variety of green wind ampelopsis grossedentata |
-
2017
- 2017-05-12 CN CN201710334159.0A patent/CN106942066B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103891613A (en) * | 2014-03-24 | 2014-07-02 | 湖北民族学院 | Rapid propagation method for rejuvenation of variety of green wind ampelopsis grossedentata |
Non-Patent Citations (2)
| Title |
|---|
| 农业部农药检定所: "《植物生长调节剂规范使用知识》", 31 January 2014 * |
| 陈加利等: "宽筋藤组织培养的初步研究", 《中国农学通报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107182793A (en) * | 2017-07-25 | 2017-09-22 | 广西壮族自治区中国科学院广西植物研究所 | A kind of tissue culture and rapid propagation method of precious jade anther-wings stem Cissus repens |
| CN115005096A (en) * | 2022-05-26 | 2022-09-06 | 桂林医学院 | Ethnic medicine alangium platanifolium seedling breeding method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106942066B (en) | 2018-11-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102405759B (en) | Cuttage seedling culturing method for branch culture of honeysuckle | |
| CN106718890B (en) | A kind of method for tissue culture suitable for Chinese herbal medicine murraya paniculataJack seedling | |
| Arya et al. | Micropropagation of Leptadenia reticulata—a medicinal plant | |
| CN105594593A (en) | Radix tetrastigme lateral branch induced seedling cultivation method | |
| CN106358821A (en) | Cuttage treatment method in cuttage seedling culturing process of honeysuckles | |
| CN110558226A (en) | In-vitro culture rapid propagation method of Ardisia gigantea | |
| CN106942066A (en) | A kind of tissue culture and rapid propagation method of Guangxi characteristic Yao nationality medicine treebine stem | |
| CN105766654A (en) | Tissue culture method for artocarpus nanchuanensis | |
| CN103229716A (en) | Method for seed selection of excellent honeysuckle flower Lonicera japonica Thunb II variety | |
| CN103636506B (en) | method for performing plant culture by utilizing shepherdia argentea caulicle regenerated plant induction culture medium and | |
| CN111034615B (en) | Tissue culture method for rapid propagation of alum root "tiramisu | |
| Anburaj et al. | In vitro regeneration of Cleome viscosa–an important medicinal herb | |
| CN106962196B (en) | A kind of tissue culture and rapid propagation method of Chinese medicine sinomenium acutum | |
| CN105432466B (en) | Method with plant regeneration occurs for a kind of pittosporum tobira somatic embryo | |
| CN107041310B (en) | A kind of tissue culture and rapid propagation method of ethnic drug fevervine | |
| CN106900553B (en) | A kind of tissue culture and rapid propagation method of civil medicinal and edible plant Erythropalum Scandens Blume | |
| CN106613999B (en) | The tissue culture and rapid propagation method of one seedling medicine Heiguteng exract | |
| CN105409779A (en) | Tissue culture rapid reproduction method for Cinnamomum kanehirae | |
| CN105532452A (en) | Method for inducing and quickly breeding daphnemezereum seeds | |
| Shukla et al. | In-vitro mass multiplication of a threatened tree fern, Cyathea spinulosa Wall. ex Hook | |
| CN106922539A (en) | A kind of tissue culture and rapid propagation method of Chinese medicine cissus assamica | |
| CN108738772B (en) | Cutting seedling breeding method for schefflera octophylla | |
| CN106900554A (en) | A kind of tissue culture and rapid propagation method of Chinese medicine Spatholobus sinensis chum et T. Chen | |
| CN108184666B (en) | A kind of method for inspiring xylophyta bearing tree and sprouting the method and the quick breeding seedling based on juvenile form bud of juvenile form bud | |
| CN106577286B (en) | A kind of method of pipex kadsura tissue-culturing rapid propagation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20181127 Termination date: 20200512 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |