CN106937749A - The method that bean dregs are modified with monascus - Google Patents

The method that bean dregs are modified with monascus Download PDF

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Publication number
CN106937749A
CN106937749A CN201710175346.9A CN201710175346A CN106937749A CN 106937749 A CN106937749 A CN 106937749A CN 201710175346 A CN201710175346 A CN 201710175346A CN 106937749 A CN106937749 A CN 106937749A
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China
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bean dregs
monascus
dregs
seeded
seed liquor
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Inventor
李兴江
李传运
郑志
吴学凤
姜绍通
李顺
顾永忠
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Hefei University of Technology
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Hefei University of Technology
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/152Milk preparations; Milk powder or milk powder preparations containing additives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38Other non-alcoholic beverages
    • A23L2/382Other non-alcoholic beverages fermented
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Beans For Foods Or Fodder (AREA)

Abstract

The present invention provides a kind of method being modified with monascus to bean dregs, including step:(1) actication of culture:The monascus specie for the production haematochrome that inclined-plane is preserved is seeded in the PDA culture medium sterilized, obtains activated spawn;(2) preparation of seed liquor:Activated spawn is seeded in the liquid PDA culture medium sterilized, be placed at a temperature of 27~30 DEG C, shaking table culture 4~6 days obtains seed liquor;(3) ferment:Seed liquor is seeded in treated bean dregs, is placed in shaking table and cultivates, obtain fermenting bean dregs liquid.The soybean processing accessory substance bean dregs that the present invention is largely discarded by the use of the monascus for producing haematochrome as fermented bacterium to ferment, compared with non-fermented soybean dregs, red cellulose content is greatly increased, color value is significantly improved, soluble dietary fibre content is improved by about one time, haematochrome increase is conducive to extending the shelf life, and the color and luster for the food being processed into, bean dregs nutritive value is greatly increased.

Description

The method that bean dregs are modified with monascus
Technical field
The invention belongs to technical field of food biotechnology, and in particular to a kind of to produce dietary fiber by fermenting raw materials of bean dregs Method.
Background technology
Soybean is to produce bean dregs during main cereals, soybean processing.The annual soybean processing of China according to statistics The bean dregs of generation can all reach more than ten million ton.Bean dregs are due to that should not store, coarse mouthfeel, beany flavor weight, and dry power consumption Greatly, thus food enterprise is general bean dregs is inexpensively sold to local peasant household or feed is regarded by plant, it is having or even directly discard. And rich in dietary fiber, protein, fat, isoflavones and mineral matter etc. in bean dregs, with abundant nutritive value, and bean dregs Dietary fiber content is up to 59.87%, and wherein soluble dietary fibre content is only 6%-7%, and is played a role in human body Mainly soluble dietary fiber, relatively low soluble dietary fibre content reduces the value of bean dregs.The meals of bean dregs Food fiber can prevent some chronic diseases such as obesity, high fat of blood, diabetes etc., as people's living standard is improved constantly, Chronic disease is also increasingly common, and in bean dregs dietary fiber utilization, especially the utilization of soluble dietary fiber to improve meals Eat structure significant.
Bean dregs are due to color and luster yellowing and poor taste, and the food being processed into often less is esthetically acceptable to the consumers, at present, to bean dregs Carry out quality-improving method studies have reported that, mainly including chemical method, enzyme process and Physical, fermentation method also has been reported that.Change Method, cost is relatively low, but there is also side reaction is more, the pollution of complex technical process, chemical reagent, the more difficult control of polysaccharide hydrolysis degree The defects such as system.Enzyme process, cost is higher, and need to select suitable enzyme.Physical, cost needed for generally requiring high-temperature heating is higher, Power consumption is big.And the method for Fermentation Condition of Monascus spp of the present invention, simple to operate, environmental protection, and required low cost, it is treated Bean dregs production has haematochrome, improves the color and luster of bean dregs, improves soluble dietary fibre content, is more beneficial for widening bean dregs in food Utilization scope in product processing.
The content of the invention
Bean dregs are modified by the weak point existed for this area the purpose of the present invention is to propose to one kind with monascus Method.
Interests purpose of the present invention is to propose the bean dregs modified product that methods described is obtained.
The technical scheme for realizing above-mentioned purpose of the present invention is:
A kind of method that bean dregs are modified with monascus, including step:
(1) actication of culture:The monascus specie for the production haematochrome that inclined-plane is preserved is seeded to the PDA culture medium sterilized In, cultivated 3~5 days at a temperature of 27~30 DEG C, obtain activated spawn;
(2) preparation of seed liquor:Activated spawn in step (1) is seeded in the liquid PDA culture medium sterilized, connect Kind of amount is 0.03%~0.05% (volume ratio), is placed in 27~30 DEG C of shaking tables 4~6 days, obtains seed liquor;
(3) ferment:The seed liquor prepared in step (2) is seeded in treated bean dregs, is placed in shaking table and trains Support, obtain fermenting bean dregs liquid.
Wherein, described bean dregs cross grit number for 80~100 mesh.
Wherein, the processing mode of bean dregs is in step (3):The mass ratio of boiling after adding water, bean dregs and water is 1:1.3~1: 1.5。
Further, in described step (3) bean dregs processing mode thermophilic digestion, boiling temperature be 120 DEG C;During boiling Between be 15~25min.
Wherein, glucose is added with described step (3) in bean dregs, the addition of glucose is the 3%- of bean dregs quality 5%.
Wherein, inoculum concentration is 5%-7% (volume ratio) in described step (3).
Preferably, fermentation temperature is 28 DEG C -29 DEG C in described step (3);Fermentation time is 5-7 days.
Further, fermenting bean dregs liquid step (3) obtained obtains the fermented soybean dregs powder of solid, the hair through drying Ferment okara powder can be used to substitute or part substitutes flour and wheaten food is made.
Or, the fermenting bean dregs liquid that step (3) is obtained is mixed with fermented dairy product with sterilized milk, or beverage is made.
The bean dregs modified product that the method for the invention is obtained.
The beneficial effects of the present invention are:
Use Fermentation Condition of Monascus spp bean dregs production haematochrome proposed by the invention and the side for improving its soluble dietary fibre content Method, had both improved the content of soluble dietary fiber in bean dregs, added the nutritive value of bean dregs, bean dregs is generated red Element, is conducive to bean dregs foodstuff processing to be received by consumer, is also beneficial to the processing of enterprise, lifts agricultural product accessory substance added value, increases Plus enterprise competitiveness.
The soybean processing accessory substance that the present invention is largely discarded by the use of the monascus for producing haematochrome as fermented bacterium to ferment Bean dregs, compared with non-fermented soybean dregs, red cellulose content is greatly increased, and color value is significantly improved, and soluble dietary fibre content is improved By about one time, haematochrome increase is conducive to extending the shelf life, and the color and luster for the food being processed into, bean dregs nutritive value increases greatly Plus, it can effectively prevent the angiocardiopathies such as human body hypertension, heart disease.
Embodiment
Following examples are used to illustrate the present invention, but are not limited to the scope of the present invention.
Monascus ruber (the Monascus rubber SE1703) bacterial strain used is purchased from China typical culture collection center (CCTCC (Wuhan University), address:Wuchang, wuhan Luo Jia Shan), preserving number CCTCCNO:M2013082.
In embodiment, unless otherwise specified, used method is the conventional method in this area.
Embodiment 1
The bean dregs that the present embodiment is used is the leftover bits and pieces of Soybean production bean product, the moisture content 84% of bean dregs, wherein containing meals Fiber 59.87% is eaten, soluble dietary fibre content is 7.3%, protein content 16.9%.Bean dregs cross 90 mesh sieves, standby.
(1) actication of culture:The monascus specie for the production Monascin that inclined-plane is preserved is seeded to the PDA culture medium sterilized In, cultivated 3 days at a temperature of 28 DEG C, obtain activated spawn;
(2) preparation of seed liquor:Activated spawn in step (1) is seeded in the liquid PDA culture medium sterilized, connect The amount of kind is 0.05%, is placed at a temperature of 28 DEG C, shaking table culture 4 days, obtains seed liquor, and concentration is 6 × 107CFU/ml。
(3) ferment:Bean dregs are 1 with water quality ratio:1.3, add after water and glucose in 120 DEG C of boiling 20min, according to beans Slag gauge, the addition of glucose is 3%,
The seed liquor prepared in step (2) is seeded in the bean dregs after boiling, inoculum concentration is 3% (volume ratio), It is placed at a temperature of 28 DEG C, shaking table culture 6 days obtains fermenting bean dregs liquid.
Soluble dietary fibre content in bean dregs is measured after fermentation with enzymatic gravimetric method (GB 5009.88-2014), measures and reaches 10.95%, protein content 18.3% is measured, the present invention carries out fermentation to bean dregs using monascus and improves the soluble meals of bean dregs The amount of fiber is eaten, 50% is improved.
After being centrifuged to zymotic fluid, reference solution is made with 70% ethanol, absorbance, warp are measured with spectrophotometric under 505nm Calculate color value and reached 8.53U/ml, the present invention is fermented to bean dregs using monascus, improves the color and luster of bean dregs, make red Cellulose content is greatly increased,
(4) processing and utilization of zymotic fluid:By the fermenting bean dregs liquid obtained in step (3) through drying process, solid is obtained The fermented soybean dregs powder rich in soluble dietary fiber, be added in flour, make the Flour product rich in soluble dietary fiber.
The fermenting bean dregs liquid obtained in step (3) is directly added into milk, makes the leben rich in dietary fiber.
Embodiment 2:
The bean dregs that the present embodiment is used is the solid bean dregs of Soybean production soya-bean milk, moisture content 83%, wherein containing dietary fiber 59.87%, it is 7.3%, protein content 17.6% containing soluble dietary fibre content.Bean dregs cross 100 mesh sieves, standby.
(1) actication of culture:The monascus specie for the production Monascin that inclined-plane is preserved is seeded to the PDA culture medium sterilized In, cultivated 3 days at a temperature of 28 DEG C, obtain activated spawn;
(2) preparation of seed liquor:Activated spawn in step (1) is seeded in the liquid PDA culture medium sterilized, connect Kind of amount is 0.05%, is placed in 28 DEG C of shaking table cultures 4 days, obtains seed liquor, concentration is 6.5 × 107CFU/ml;
(3) ferment:Bean dregs are 1 with water quality ratio:1.4, add after water and glucose in 120 DEG C of boiling 20min, according to beans Slag gauge, the addition of glucose is 4%,
The seed liquor prepared in step (2) is seeded in treated bean dregs, inoculum concentration is 4%, is placed in 29 DEG C Shaking table culture 7 days, obtains fermenting bean dregs liquid.
It is 11.1% that soluble dietary fibre content in bean dregs is measured with enzymatic gravimetric method (GB 5009.88-2014), is measured Protein content 18.8%, the present invention carries out the amount that fermentation improves bean dregs soluble dietary fiber to bean dregs using monascus, Improve 52%.
After being centrifuged to zymotic fluid, reference solution is made with 70% ethanol, absorbance, warp are measured with spectrophotometric under 505nm Calculate color value and reached 8.02U/ml, the present invention is fermented to bean dregs using monascus, improves the color and luster of bean dregs, make red Cellulose content is greatly increased,
(4) processing and utilization of zymotic fluid:The fermenting bean dregs liquid obtained in step (3) is added to face after drying process In powder, the Flour product rich in soluble dietary fiber is made;Or it is used directly to cook the zymotic fluid of fermented dairy product, obtain being rich in meals Eat the leben of fiber.
Embodiment 3:Haematochrome and the method for improving its soluble dietary fibre content are produced using bean dregs
The bean dregs that the present embodiment is used is the solid bean dregs of Soybean production soya-bean milk, moisture content 83%, wherein containing dietary fiber 59.87%, wherein soluble dietary fibre content is 7.3%, protein content 17.1%.Bean dregs cross 100 mesh sieves, standby.
(1) actication of culture:The monascus specie for the production Monascin that inclined-plane is preserved is seeded to the PDA culture medium sterilized In, cultivated 3 days at a temperature of 28 DEG C, obtain activated spawn;
(2) preparation of seed liquor:Activated spawn in step (1) is seeded in the liquid PDA culture medium sterilized, connect The amount of kind is 0.05%, is placed at a temperature of 28 DEG C, shaking table culture 4 days, obtains seed liquor, and concentration is 8.0 × 107CFU/ml;
(3) ferment:Bean dregs are 1 with water quality ratio:1.5, add after water and glucose in 120 DEG C of boiling 20min, according to beans Slag gauge, the addition of glucose is 3%,
The seed liquor prepared in step (2) is seeded in treated bean dregs, inoculum concentration is 5%, is placed in 30 DEG C Shaking table culture 8 days, obtains fermenting bean dregs liquid.
It is 11.02% that soluble dietary fibre content in bean dregs is measured with enzymatic gravimetric method (GB 5009.88-2014), is measured Protein content 17.9%, the present invention carries out the amount that fermentation improves bean dregs soluble dietary fiber to bean dregs using monascus, Improve 51%.
After being centrifuged to zymotic fluid, reference solution is made with 70% ethanol, absorbance, warp are measured with spectrophotometric under 505nm Calculate color value and reached 7.81U/ml, the present invention is fermented to bean dregs using monascus, improves the color and luster of bean dregs, make red Cellulose content is greatly increased.
(4) processing and utilization of zymotic fluid:The fermenting bean dregs liquid obtained in step (3) is added to face after drying process In powder, the Flour product rich in soluble dietary fiber is made;Or it is used directly to cook the zymotic fluid of fermented dairy product, make rich in meals Eat the leben of fiber.
Comparative example
, as raw material, to be with lactobacillus bulgaricus and streptococcus thermophilus (mass ratio 1: 1) with the identical bean dregs of embodiment 1 Leavening, soybean dietary fiber is extracted using fermentation method, and it is 12.2%, the color value of zymotic fluid to obtain soluble dietary fibre content As before fermentation, 0.034U/ml is, haematochrome is not produced.
And the present invention utilizes Fermentation Condition of Monascus spp bean dregs, the content of the soluble dietary fiber of bean dregs is set to bring up to 10- While 11.12%, bean dregs color value reaches 7.81U/ml-8.53U/ml, has so both added the nutritive value of bean dregs, improves again The color and luster of bean dregs, is conducive to the processing and utilization of bean dregs.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (10)

1. a kind of method that bean dregs are modified with monascus, it is characterised in that including step:
(1) actication of culture:The monascus specie for the production haematochrome that inclined-plane is preserved is seeded in the PDA culture medium sterilized, Cultivated 3~5 days at a temperature of 27~30 DEG C, obtain activated spawn;
(2) preparation of seed liquor:Activated spawn in step (1) is seeded in the liquid PDA culture medium sterilized, inoculum concentration It is by volume 0.03%~0.05%, shaking table culture 4~6 days, obtain seed liquor at a temperature of being placed in 27~30 DEG C;
(3) ferment:The seed liquor prepared in step (2) is seeded in treated bean dregs, is placed in shaking table and cultivates, obtain Fermenting bean dregs liquid.
2. according to the method described in claim 1, it is characterised in that described bean dregs cross grit number for 80~100 mesh.
3. according to the method described in claim 1, it is characterised in that the processing mode of bean dregs is in step (3):Steamed after adding water Boil, the mass ratio of bean dregs and water is 1:1.3~1:1.5.
4. according to the method described in claim 1, it is characterised in that the processing mode high temperature of bean dregs steams in described step (3) Boil, boiling temperature is 120 DEG C;Digestion time is 15~25min.
5. method according to claim 3, it is characterised in that be added with glucose before bean dregs boiling, glucose adds Dosage is the 3%~5% of bean dregs quality.
6. according to the method described in claim 1, it is characterised in that inoculum concentration is 5%~7% in described step (3).
7. according to the method described in claim 1, it is characterised in that fermentation temperature is 28~29 DEG C in described step (3);Hair The ferment time is 5~7 days.
8. the method according to any one of claim 1~7, it is characterised in that the fermenting bean dregs liquid for obtaining step (3) is passed through Dry, obtain the fermented soybean dregs powder of solid, for wheaten food to be made, the fermented soybean dregs powder is used for substituting or part substitutes flour Wheaten food is made.
9. the method according to any one of claim 1~7, it is characterised in that the fermenting bean dregs liquid for obtaining step (3) with Sterilized milk is mixed with fermented dairy product, or beverage is made.
10. the bean dregs modified product obtained according to any one of claim 1~9 methods described.
CN201710175346.9A 2017-03-22 2017-03-22 The method that bean dregs are modified with monascus Pending CN106937749A (en)

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Cited By (7)

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CN108094534A (en) * 2017-12-22 2018-06-01 扬州大学 A kind of production method of bean dregs Yoghourt
CN109287905A (en) * 2018-09-20 2019-02-01 湖南景湘源食品饮料有限公司 Fermented type soybean residue beverage preparation method
CN110692739A (en) * 2019-11-15 2020-01-17 肖顺治 Novel bean dreg fermentation processing method
CN111139190A (en) * 2020-02-19 2020-05-12 福建大北农水产科技有限公司 Monascus strain, fermented soybean meal thereof and functional biological feed for aquatic products
CN111955556A (en) * 2020-08-31 2020-11-20 合肥工业大学 Preparation method of red yeast dried bean curd
CN114041561A (en) * 2021-11-18 2022-02-15 江南大学 Method for producing low-GI red yeast powder by fermenting yellow wine lees with monascus
CN115005451A (en) * 2022-06-20 2022-09-06 辽宁豆华天宝食品科技股份有限公司 Method for extracting soluble dietary fiber from bean dregs

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108094534A (en) * 2017-12-22 2018-06-01 扬州大学 A kind of production method of bean dregs Yoghourt
CN108094534B (en) * 2017-12-22 2021-05-04 扬州大学 Production method of bean dreg yoghourt
CN109287905A (en) * 2018-09-20 2019-02-01 湖南景湘源食品饮料有限公司 Fermented type soybean residue beverage preparation method
CN110692739A (en) * 2019-11-15 2020-01-17 肖顺治 Novel bean dreg fermentation processing method
CN111139190A (en) * 2020-02-19 2020-05-12 福建大北农水产科技有限公司 Monascus strain, fermented soybean meal thereof and functional biological feed for aquatic products
CN111955556A (en) * 2020-08-31 2020-11-20 合肥工业大学 Preparation method of red yeast dried bean curd
CN114041561A (en) * 2021-11-18 2022-02-15 江南大学 Method for producing low-GI red yeast powder by fermenting yellow wine lees with monascus
CN115005451A (en) * 2022-06-20 2022-09-06 辽宁豆华天宝食品科技股份有限公司 Method for extracting soluble dietary fiber from bean dregs

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