CN106937698B

CN106937698B - Method and device for extracting rhizoma Polygonati extract, and animal feed with antivirus and growth promoting effects

Info

Publication number: CN106937698B
Application number: CN201710315519.2A
Authority: CN
Inventors: 刘汉伟; 胡玲; 陈有为; 杨胜祥
Original assignee: NINGBO ACADEMY OF SCIENCE AND TECHNOLOGY FOR INSPECTION AND QUARANTINE; Zhejiang A&F University ZAFU; Ningbo College of Health Sciences
Current assignee: NINGBO ACADEMY OF SCIENCE AND TECHNOLOGY FOR INSPECTION AND QUARANTINE; Zhejiang A&F University ZAFU; Ningbo College of Health Sciences
Priority date: 2017-05-08
Filing date: 2017-05-08
Publication date: 2020-07-31
Anticipated expiration: 2037-05-08
Also published as: CN106937698A

Abstract

The invention discloses an extraction method of a polygonatum sibiricum extract, which comprises the following steps: (1) grinding the cleaned and dried rhizome of the polygonatum sibiricum into powder, and sieving the powder by a sieve of 70-80 meshes to obtain polygonatum sibiricum powder; (2) leaching rhizoma polygonati powder for 60-80 min by purified water at 90-100 ℃, filtering, and concentrating the filtrate to obtain a concentrated solution A and filter residue A; (3) drying the filter residue A in an environment of 40-50 ℃ to obtain a filter residue B; (4) adding an ethanol solution with the volume ratio of 5:1 to the filter residue B into the filter residue B, leaching for 20-30 min, filtering, and concentrating the filtrate to obtain a concentrated solution B, wherein the mass concentration of the ethanol solution is 80-95%; (5) mixing the concentrated solution A and the concentrated solution B, and concentrating to obtain extract. The method has the advantages of simple operation and high polygonatum polysaccharide yield.

Description

Method and device for extracting rhizoma Polygonati extract, and animal feed with antivirus and growth promoting effects

Technical Field

The invention relates to the field of extracting effective components in rhizoma polygonati, in particular to an extraction method and an extraction device of a rhizoma polygonati extract and an animal feed with antiviral and growth-assisting functions.

Background

Rhizoma Polygonati is sweet, tasty, and rich in starch, sugar, fat, protein, carotene, vitamins, and other nutrients. The sealwort can be eaten raw or stewed, can increase the strength of human body, and has abundant muscles and strong bone marrow after long-term eating, and is very beneficial to human body.

Wherein, the sealwort contains a plurality of effective components, such as sealwort polysaccharide which is an important effective component. The rhizoma Polygonati polysaccharide has effects of lowering blood sugar, clearing away heat and toxic materials, and consolidating constitution. Therefore, how to extract polygonatum polysaccharide from polygonatum more quickly, more simply and with higher yield is an urgent problem to be solved.

In the traditional extraction of polygonatum polysaccharide, a water extraction method is generally adopted to extract polysaccharide nutrient substances in polygonatum, however, only the water extraction method is adopted, the yield of polygonatum polysaccharide is very low, and the extracted polygonatum residue still contains a large amount of polygonatum polysaccharide, so that the waste is very serious.

In addition, in the leaching process, polygonatum powder is generally placed in a container containing a solvent, and then is placed in an ultrasonic extraction device for ultrasonic extraction, so that polygonatum polysaccharide is separated out, however, the existing ultrasonic extraction device generally has no stirring device, and the extraction effect of polygonatum particles in the middle of the container is poor, so that polygonatum polysaccharide in polygonatum particles is not completely separated out.

Disclosure of Invention

The invention provides an extraction method of a polygonatum sibiricum extract, which has the advantages of simple operation and high polygonatum sibiricum extract yield.

In order to achieve the purpose, the invention provides the following technical scheme:

a method for extracting rhizoma Polygonati extract comprises the following steps:

(1) grinding the cleaned and dried rhizome of the polygonatum sibiricum into powder, and sieving the powder by a sieve of 70-80 meshes to obtain polygonatum sibiricum powder;

(2) leaching rhizoma polygonati powder for 60-80 min by purified water at 90-100 ℃, filtering, and concentrating the filtrate to obtain a concentrated solution A and filter residue A;

(3) drying the filter residue A in an environment of 40-50 ℃ to obtain a filter residue B;

(4) adding an ethanol solution with the volume ratio of 5:1 to the filter residue B into the filter residue B, leaching for 20-30 min, filtering, and concentrating the filtrate to obtain a concentrated solution B, wherein the mass concentration of the ethanol solution is 80-95%;

(5) and mixing the concentrated solution A and the concentrated solution B, and concentrating to obtain an extract to obtain the sealwort extract.

Wherein, firstly, the water extraction method is adopted to extract the sealwort extract, which can separate out most of polysaccharide in the sealwort, and after the extraction is finished, the ethanol solution is adopted to extract the polysaccharide in the filter residue again, so that the polysaccharide substance yield in the sealwort is higher.

More specifically, the step (5) adopts reduced pressure distillation concentration. Reduced pressure distillation can reduce the time taken for the entire extraction process.

The invention also provides a polygonatum sibiricum extract obtained by the extraction method. The polygonatum extract contains polygonatum polysaccharide and other effective components.

The invention also provides an extraction device of the polygonatum sibiricum extract, which comprises a liquid storage tank and a columnar container, wherein the liquid storage tank is used for loading a solvent, the opening end of the liquid storage tank is upward, the columnar container is used for loading polygonatum sibiricum powder, the side wall of the liquid storage tank is provided with a water outlet for discharging internal liquid, the liquid storage tank is provided with a fixing frame, the inner wall of the liquid storage tank is provided with a fixing frame for fixing the columnar container, the extraction device also comprises a plug and an operation handle, the plug is slidably arranged in the hollow part of;

one end of the columnar container, which is back to the operating handle part, is provided with a filter layer which is used for leading the solvent in the liquid storage tank to enter the columnar container and preventing the sealwort powder in the columnar container from entering the liquid storage tank,

the corresponding position of the liquid storage tank wall and the columnar container is provided with a fixed seat used for sealing and fixing the filter layer.

Wherein, the staff can hand the handle portion of operation, through making a round trip to push and pull the end cap that is located the inside of column container for the solvent in the liquid storage tank gets into from the filter layer, perhaps flows out from the filter layer through the leaching liquor of leaching sealwort, and the repeated operation can accelerate sealwort extract product to dissolve in the solvent, makes the yield of sealwort extract product higher.

Preferably, the filter layer is gauze which is provided with cotton in the middle and is enclosed into a column shape. The gauze is comparatively soft, and the price is also cheaper, presss from both sides between two-layer gauze and is equipped with the cotton, and the cotton can play filterable effect for solvent and solute are separately, thereby when making extraction work accomplish, and the staff need not do further filtering work to the extract that obtains, thereby greatly reduced operating time.

Preferably, sealwort extract product extraction element still includes the connector of fixing gauze and fixed seat mutually, and the cavity position of gauze can hold the connector, the connector outer wall is equipped with the external screw thread, and is corresponding, the fixed seat is equipped with the recess of adaptation connector, and the recess cell wall is equipped with the internal thread with external screw thread looks adaptation. During the use, with the gauze cover in the connector outside, because of passing through threaded fixation between connector and the fixing base, the event is twisted into between fixing base and the connector with the gauze and can make it can not drop at the extraction in-process, and fixed effect is better.

Preferably, the sealwort extract extracting device further comprises a driver for driving the handle to move telescopically. The driver can accelerate the movement of the operating handle, and the automation of the extraction process is realized without human participation.

Preferably, the driver is an air cylinder, and the output end of the air cylinder is fixedly connected with the operating handle.

The invention also provides an antiviral and growth-promoting animal feed which comprises the following components in parts by weight: 20-50 parts of soybean meal, 20-40 parts of rice bran, 10-20 parts of cornstalks, 5-15 parts of ryegrass, 10-20 parts of anoectochilus roxburghii, 5-10 parts of madder, 3-5 parts of wolfberry fruits, 15-20 parts of plantain, 10-15 parts of radix tetrastigme, 15-30 parts of isatis roots, 5-10 parts of black nightshade, 15-25 parts of cocklebur and 10-20 parts of feed additives;

the feed additive comprises the rhizoma polygonati extract and vitamins;

wherein the mass ratio of the polygonatum sibiricum extract to the vitamins is 4: 1.

Rhizoma Polygonati has effects of promoting growth and promoting animal growth; the radix isatidis, the radix tetrastigme and the anoectochilus formosanus have the functions of clearing heat, removing toxicity and improving the immunity of animals, so that the rhizoma polygonati, the radix isatidis and the radix tetrastigme are combined together to be used as one component in the animal feed provided by the invention, the synergistic effect is achieved, the animals eating the feed can grow faster, and are less prone to being infected by germs.

Preferably, the vitamins include one or more of vitamin A, vitamin D, vitamin E, vitamin B1 and vitamin B2.

The invention has the beneficial effects that:

(1) in the invention, the water extraction method is firstly adopted to extract the sealwort extract in the sealwort, so that most of polysaccharide in the sealwort can be separated out, and after the extraction is finished, the ethanol solution is adopted to carry out extraction operation on the polysaccharide in the filter residue again, so that the yield of polysaccharide substances in the sealwort is higher.

(2) In the invention, a worker can hold the handle by hand and push and pull the plug positioned in the cylindrical container back and forth, so that a solvent in the liquid storage tank enters from the filter layer, or leaching liquor for leaching rhizoma polygonati flows out from the filter layer, and the dissolution of the rhizoma polygonati extract in the solvent can be accelerated by repeated operation, and the yield of the rhizoma polygonati extract is higher.

(3) Rhizoma Polygonati has effects of promoting growth and promoting animal growth; the radix isatidis, the radix tetrastigme and the anoectochilus formosanus have the functions of clearing heat, removing toxicity and improving the immunity of animals, so that the rhizoma polygonati, the radix isatidis and the radix tetrastigme are combined together to be used as one component in the animal feed provided by the invention, the synergistic effect is achieved, the animals eating the feed can grow faster, and are less prone to being infected by germs.

Drawings

FIG. 1 is a schematic diagram of an apparatus for extracting Polygonati officinalis rhizoma extract of this example 1;

FIG. 2 is a schematic diagram of an apparatus for extracting Polygonati officinalis rhizoma extract of this example 1;

FIG. 3 is a schematic structural diagram of the fixing base, the connecting head, and the filter layer in this embodiment 1;

fig. 4 is an enlarged view of a portion a in fig. 3.

In the figure: 61. liquid storage tank, 62, driver, 63, cylinder, 64, mount, 65, filter layer, 66, fixing base, 67, column container, 68, operation stalk portion, 69, end cap, 70, connector, 71, delivery port.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The embodiment provides a technical scheme:

as shown in fig. 1 to 3, an extraction device for polygonatum sibiricum extract comprises a liquid storage tank 61 for loading solvent and having an upward opening end, and a cylindrical container 67 for loading polygonatum sibiricum powder, wherein a water outlet 71 for discharging internal liquid is formed in the side wall of the liquid storage tank 61, a fixing frame 64 for fixing the cylindrical container 67 is formed in the inner wall of the liquid storage tank 61, the extraction device further comprises a plug 69 slidably penetrating through the hollow part of the cylindrical container 67, and an operating handle 68 for facilitating force application of a worker, and the plug 69 is fixedly connected with the operating handle 68;

one end of the columnar container 67, which is back to the operating handle 68, is provided with a filter layer 65 which is used for allowing the solvent in the liquid storage tank 61 to enter the columnar container 67 and preventing rhizoma polygonati powder in the columnar container 67 from entering the liquid storage tank 61,

a fixing seat 66 for sealing and fixing the filter layer 65 is arranged at the corresponding position of the liquid storage tank 61 and the columnar container 67.

Wherein, the staff can hold the handle 68, push and pull back the end cap 69 inside the cylindrical container 67 through making a round trip to make the solvent in the liquid storage tank 61 enter from the filter layer 65, or flow out from the filter layer through the leaching liquor of leaching sealwort, operate repeatedly, can accelerate the sealwort extract to dissolve in the solvent, make the yield of sealwort extract higher.

In this embodiment, filter layer 65 is the gauze, and the gauze is comparatively soft, and the price is also cheap, presss from both sides between two-layer gauze and is equipped with the cotton, and the cotton can play filterable effect for solvent and solute are separately, thereby when making extraction work accomplish, and the staff need not do further filtering work to the extract that obtains, thereby greatly reduced operating time.

As shown in fig. 3, in the present embodiment, when in use, the gauze is sleeved outside the connector 70, and the connector 70 and the fixing base 66 are fixed by the screw threads, so that the gauze can be screwed into the space between the fixing base 66 and the connector 70 to prevent the gauze from falling off during the extraction process, thereby achieving a better fixing effect.

As shown in fig. 1, the drive can accelerate the movement of the handle 68, enabling automation of the extraction process without human intervention.

As shown in fig. 1, the driver mentioned in this embodiment is a cylinder 63, and the output end of the cylinder 63 is fixedly connected with an operating handle 68.

As shown in fig. 1, the number of the fixing frames 64 is plural, the plurality of fixing frames 64 are arranged in a straight line, and two adjacent fixing frames 64 form a space for placing a reagent bottle.

As shown in fig. 4, the end of the connector 70 is provided with a radius 72, and correspondingly, the end of the fixing seat 66 is provided with a groove matched with the radius 72 at the end of the connector 70, so that the connector 70 and the fixing seat 66 are more attached to each other, and the connector 70 and the fixing seat 66 are prevented from being screwed tightly, and the connector 70 is not easy to take out.

Example 2

An antiviral and growth-promoting animal feed, which comprises the following components in parts by weight: 20 parts of soybean meal, 20 parts of rice bran, 10 parts of corn stalks, 5 parts of ryegrass, 10 parts of anoectochilus roxburghii, 5 parts of madder, 3 parts of wolfberry fruit, 15 parts of plantain herb, 10 parts of radix tetrastigme, 15 parts of isatis root, 5 parts of black nightshade, 15 parts of xanthium sibiricum and 10 parts of feed additive;

the feed additive comprises the polygonatum extract and vitamins;

Example 3

An antiviral and growth-promoting animal feed, which comprises the following components in parts by weight: 25 parts of soybean meal, 30 parts of rice bran, 15 parts of corn stalks, 10 parts of ryegrass, 15 parts of anoectochilus roxburghii, 8 parts of madder, 3 parts of wolfberry fruit, 18 parts of plantain herb, 13 parts of radix tetrastigme, 25 parts of isatis root, 8 parts of black nightshade, 20 parts of xanthium sibiricum and 15 parts of feed additive;

the feed additive consists of rhizoma polygonati extract and vitamins;

Example 4

An antiviral and growth-promoting animal feed, which comprises the following components in parts by weight: 50 parts of soybean meal, 40 parts of rice bran, 20 parts of corn stalks, 15 parts of ryegrass, 20 parts of anoectochilus roxburghii, 10 parts of madder, 5 parts of wolfberry fruit, 20 parts of plantain herb, 15 parts of radix tetrastigme, 30 parts of isatis root, 10 parts of black nightshade, 25 parts of xanthium sibiricum and 20 parts of feed additive;

the feed additive consists of rhizoma polygonati extract and vitamins;

Example 5

the feed additive is vitamin.

Example 6

An antiviral and growth-promoting animal feed, which comprises the following components in parts by weight: 25 parts of soybean meal, 30 parts of rice bran, 15 parts of corn stalk, 10 parts of ryegrass, 15 parts of anoectochilus roxburghii, 8 parts of madder, 3 parts of wolfberry fruit, 18 parts of plantain herb, 8 parts of black nightshade, 20 parts of cocklebur and 15 parts of feed additive;

the feed additive consists of rhizoma polygonati extract and vitamins;

Example 7

An antiviral and growth-promoting animal feed, which comprises the following components in parts by weight: 25 parts of soybean meal, 30 parts of rice bran, 15 parts of corn stalks, 10 parts of ryegrass, 15 parts of anoectochilus roxburghii, 8 parts of madder, 3 parts of wolfberry fruit, 18 parts of plantain herb, 25 parts of isatis root, 8 parts of black nightshade, 20 parts of cocklebur and 15 parts of feed additive;

the feed additive consists of rhizoma polygonati extract and vitamins;

Example 8

An antiviral and growth-promoting animal feed, which comprises the following components in parts by weight: 25 parts of soybean meal, 30 parts of rice bran, 15 parts of corn stalks, 10 parts of ryegrass, 15 parts of anoectochilus roxburghii, 8 parts of madder, 3 parts of wolfberry fruit, 18 parts of plantain herb, 13 parts of radix tetrastigme, 8 parts of black nightshade, 20 parts of cocklebur and 15 parts of feed additive;

the feed additive is prepared from rhizoma Polygonati extract and vitamins;

The extraction method of the sealwort extract comprises the following steps:

(1) grinding the cleaned and dried rhizome of the polygonatum sibiricum into powder, and sieving the powder with a 80-mesh sieve to obtain polygonatum sibiricum powder;

(2) extracting rhizoma polygonati powder with purified water at 90-100 ℃ for 80min, filtering, concentrating the filtrate to obtain a concentrated solution A, and filtering residues A;

(4) adding 85% ethanol solution with a volume ratio of 1:5 to the ethanol solution into the filter residue B, leaching for 30min, filtering, and concentrating the filtrate to obtain concentrated solution B;

(5) mixing the concentrated solution A and the concentrated solution B, and concentrating to obtain extract.

The sealwort extract mentioned in example 2 to example 4, and example 6 to example 7 was prepared by the same extraction method as provided in this example.

Test example 1

80 experimental piglets with similar growth vigor and weight average body weight of animal medical college of agriculture and forestry university in Zhejiang are taken and randomly divided into 8 groups, the groups are numbered from A to H, the groups A to G of the piglets are respectively fed with the animal feed and the purified water for resisting virus and promoting growth, which are provided by the embodiments 2 to 8, 2 times a day, the animal feed amount fed each time is 250G/piglet, the purified water feeding amount is 1L/day, the groups H of the piglets are fed with the purified water with the same mass as the groups A to G of the piglets and the common feed with the same mass as the piglets, the groups A to G and H of the piglets are continuously fed for four weeks, and the statistical results after four weeks are as follows:

table 1: A-F group piglet weight statistical table

Group/weight	Before experiment (kg)	After the experiment (kg)	Weight gain (kg)	Disease (only)
					A	20.10	37.00	16.90	0
B	20.00	39.40	19.40	0
					C	19.60	38.10	18.50	0
D	19.40	34.10	14.70	1
					E	19.60	31.80	12.20	5
F	21.00	32.70	11.70	3
					G	19.80	32.10	12.30	2
H	20.00	30.30	10.30	6

As can be seen from Table 1, in the piglets fed by the piglets in the groups A to C, the piglets in the group B gain the most and gain 19.4kg, which shows that the animal feed provided by the example 2 has the best growth promoting effect on the piglets;

meanwhile, as the sealwort extract is not added into the animal feed provided by the embodiment 5, the weight gain of the piglets in the group D is obviously lower than that of the piglets in the groups A to C added with the sealwort extract, so the sealwort extract plays a critical role in promoting the growth of the piglets;

as the weight gain of piglets in groups E-G is obviously lower than that of piglets in group B (groups E-G are comparative examples of group B), the radix isatidis and radix tetrastigme have synergistic effect with the rhizoma polygonati extract in the feed provided by the invention, so that the feed has better growth promoting effect on the piglets.

According to the quantity of the sick piglets of the groups A-C and E-G, the probability of the piglets suffering from the infectious diseases can be reduced by adding the isatis root and the radix tetrastigme into the feed provided by the invention;

according to the number of the sick piglets of the group F and the group G, the effect of preventing infectious diseases of the piglets is better by adding the tetrastigma hemsleyanum into the feed;

according to the quantity of the sick piglets of the groups A-C and D, the polygonatum sibiricum extract has a good effect of preventing the piglets from being sick, and has good compatibility with radix tetrastigme and radix isatidis, so that the animal feed provided by the invention also has an antiviral effect.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims

1. An extraction device of rhizoma polygonati extract comprises a liquid storage tank (61) used for loading a solvent and with an upward opening end and a columnar container (67) used for loading rhizoma polygonati powder, wherein a water outlet (71) for discharging internal liquid is formed in the side wall of the liquid storage tank (61), a fixing frame (64) used for fixing the columnar container (67) is arranged on the inner wall of the liquid storage tank (61), the extraction device is characterized by further comprising a plug (69) which is slidably arranged in the hollow part of the columnar container (67) in a penetrating mode and an operation handle part (68) convenient for workers to apply force, and the plug (69) is fixedly connected with the operation handle part (68);

one end of the columnar container (67) back to the operating handle part (68) is provided with a filter layer (65) which is used for allowing the solvent in the liquid storage tank (61) to enter the columnar container and preventing rhizoma polygonati powder in the columnar container from entering the liquid storage tank (61),

a fixed seat (66) for sealing and fixing the filter layer (65) is arranged at the position of the liquid storage tank (61) wall corresponding to the columnar container (67);

the gauze fixing device is characterized by further comprising a connector (70) for fixing the gauze and the fixing seat (66), wherein the connector (70) can be accommodated in the hollow part of the gauze, external threads are arranged on the outer wall of the connector (70), correspondingly, a groove matched with the connector (70) is formed in the fixing seat (66), and internal threads matched with the external threads are formed in the groove wall of the groove;

the filtering layer (65) is gauze which is provided with cotton in the middle and is enclosed into a column shape.

2. The extraction device of rhizoma Polygonati extract as claimed in claim 1, further comprising a driver for driving the handle (68) to move telescopically.

3. The sealwort extract extracting device according to claim 2, wherein the driver is an air cylinder (63), and the output end of the air cylinder (63) is fixedly connected with the operating handle (68).

4. An antiviral and growth-promoting animal feed is characterized by comprising the following components in parts by weight: 20-50 parts of soybean meal, 20-40 parts of rice bran, 10-20 parts of cornstalks, 5-15 parts of ryegrass, 10-20 parts of anoectochilus roxburghii, 5-10 parts of madder, 3-5 parts of wolfberry fruits, 15-20 parts of plantain, 10-15 parts of radix tetrastigme, 15-30 parts of isatis roots, 5-10 parts of black nightshade, 15-25 parts of cocklebur and 10-20 parts of feed additives;

the feed additive comprises vitamins and a polygonatum sibiricum extract obtained by adopting the polygonatum sibiricum extract extraction device in claim 1;

wherein the mass ratio of the polygonatum sibiricum extract to the vitamins is 4: 1;

the extraction method of the polygonatum sibiricum extract comprises the following steps:

(4) mixing the filter residue B with an ethanol solution according to a volume ratio of 1:5, leaching for 30min, filtering, and concentrating the filtrate to obtain a concentrated solution B; the mass concentration of the ethanol solution is 85 percent;

5. An antiviral and growth promoting animal feed as claimed in claim 4, wherein said vitamins include one or more of vitamin A, vitamin D, vitamin E, vitamin B1, vitamin B2.