CN106929453A - Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof - Google Patents
Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/32—Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters
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Abstract
The invention belongs to microbial technology field, the biological agent for the treatment of gourmet powder fermenting mother liquor is disclosed, it is prepared as follows and obtains:It is respectively 1 × 10 according to cellar culture concentration by xylose oxidation and bacillus, micrococcus luteus, coral promise cassette bacterium and cellulomonas cartae8The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 57:3‑5:2‑3:23 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent and carrier according to 23:35 mass ratio stirring mixing, is then dried, and packs, and obtains final product.Biological agent of the present invention only contains four kinds of bacterial strains, and reasonable compatibility, symbiosis is coordinated, mutually not antagonism, and long service life, sludge yield is few.
Description
Technical field
The invention belongs to microbial technology field, and in particular to the biological agent for the treatment of gourmet powder fermenting mother liquor and its preparation side
Method.
Background technology
Monosodium glutamate is a kind of food freshener being widely used, and fermentation method production monosodium glutamate will produce substantial amounts of high-concentration waste liquid,
Monosodium glutamate waste liquid COD reaches 20-80g/L, and suspension, sulfate radical and ammonia-nitrogen content are high, and pH value is low, and intractability greatly, is processed into
This high therefore many glutamate production producer fails effectively to process monosodium glutamate waste liquid, and pole is brought to environment and social development
Big harm, monosodium glutamate industry production waste liquid pollution on the environment problem is on the rise, and the improvement of monosodium glutamate waste liquid has become
Restrict the great difficult problem of glutamate production enterprise development.The main source of monosodium glutamate waste liquid is:It is useless after the extracted glutamic acid of zymotic fluid
Mother liquor or ion exchange tail washings;Various equipment in production process (tank of sizing mixing, liquefied pot, saccharifying tank, fermentation tank, extractor, in
With the tank etc. for decolourizing) scrub raffinate;Ion exchange resin is washed and regeneration liquid waste;Liquefaction is each to the fermentation etc. that is saccharified, is saccharified to
The cooling water in stage;Various condensed waters (technique such as liquefaction, saccharification, concentration).
Current Wastewater treating method mainly has physical method, chemical method and biological method.Bioremediation is
Administer the effective ways of industrial pollution water body.It is, using the effect of microorganism, to be decomposed compound.Due to biological treatment
Method is more much lower than physics, chemical method cost, and non-secondary pollution, and microorganism has stronger variability and adaptation again
Property, therefore it has turned into ideal method.Patent of invention technology " the biological system of a kind for the treatment of gourmet powder waste water before applicant
Agent ", it uses microbial bacterial agent to process waste water, and COD, ammonia nitrogen, SS removal effects are good, but there is bacterial strain species in microbial inoculum
Excessively, the possibility of bacterial strain pollution is increased, relative difficulty is cultivated, and microbial inoculum usage amount is bigger than normal, and sewage per ton needs plus 50g
Microbial inoculum, microbial inoculum easily deposits to bottom of pond, causes wadding to collect, so as to produce substantial amounts of sludge, clears up relatively difficult.
The content of the invention
In order to overcome the defect of prior art, the invention provides the biological agent for the treatment of gourmet powder fermenting mother liquor.The present invention
Additionally provide the preparation method of above-mentioned biological agent.
The present invention is achieved by the following technical solution:
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:By xylose oxidation and bacillus, M. luteus
Bacterium, coral promise cassette bacterium and cellulomonas cartae are respectively 1 × 10 according to cellar culture concentration8The bacterium solution of cuf/g, will be above-mentioned
Four kinds of bacterium solutions are according to 5-7:3-5:2-3:The volume ratio of 2-3 is mixed to get liquid bacterial agent;By liquid bacterial agent and carrier according to 2-3:
The mass ratio stirring mixing of 3-5, is then dried, and packs, and obtains final product.
Preferably, the xylose oxidation and bacillus are xylose oxidation and bacillus(Achromobacter xylosoxidans
subsp.denitrificans)ATCC 15173.
Preferably, the micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442.
Preferably, the coral promise cassette bacterium is coral promise cassette bacterium (Nocardia coralline) ACCC
40100。
Preferably, the cellulomonas cartae is cellulomonas cartae (Cellulomonas sp) CGMCC No.2788.
Preferably, the carrier is prepared according to following technique:By zeolite and kaolinite according to 2-3:The mass ratio of 1-2
Pulverization process in pulverizer is put into, the powder of 100 mesh is then ground to;By above-mentioned powder, starch and shitosan according to 3-5:
2-3:The mass ratio of 1-2 is put into agitator, 1000rpm stirring 10min, obtains mixed material, then with polystyrene microsphere
According to 1:1 mass ratio is added in comminutor, is subsequently added into the polyvinyl alcohol for accounting for the concentration of mixed material quality 30% for 6wt%
The aqueous solution, is made the particle that particle diameter is 1-2mm;Particle is dried into 30min in 80 DEG C of baking oven, then is put into and enter in sintering furnace
Row sintering, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature, obtains final product.
The invention also discloses the preparation method of above-mentioned biological agent.
Strain of the present invention belongs to known bacterial strain, can be bought from databases such as CGMCC, ATCC and ACCC
Arrive.The Amplification Culture of each strain of the invention is the cellar culture mode of this area, is not innovative point of the present invention, unknown herein
State.
The beneficial effect that the present invention is obtained mainly includes but is not limited to the following aspects:
Biological agent of the present invention obtain using carrier and bacterium solution granulation, and specific surface area is big, thalline adhesive force by force, density and water body phase
When, can suspend with water body, it is to avoid preparation density is excessive to be deposited in that microbial profile that bottom of pond causes is uneven and influence is removed
Dirty effect, moreover it is possible to reduce the yield of sludge, is conducive to the removal of the pollutant such as COD and ammonia nitrogen in waste liquid;In order to reduce to list
The dependence of one specific microbial inoculum, it is to avoid the loss that microbial inoculum pollution is caused occur, applicant develops various biological agents, mutually complementary
Fill, it is ensured that the normal operation of sewage disposal;Biological agent of the present invention only contains four kinds of bacterial strains, and reasonable compatibility, symbiosis is coordinated, mutually not
Antagonism, reduces cost, and operating procedure is also relatively easy.
Specific embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, below in conjunction with the application tool
Body embodiment, the technical scheme to the application is clearly and completely described, it is clear that described embodiment is only this Shen
Please a part of embodiment, rather than whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not having
There is the every other embodiment made and being obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:
By xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173、
Micrococcus luteus (Micrococcus luteus) ATCC 49442, coral promise cassette bacterium (Nocardia coralline)
ACCC 40100, cellulomonas cartae (Cellulomonas sp) CGMCC No.2788 respectively according to cellar culture concentration be 1 ×
108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5:3:3:3 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent with
Carrier is according to 2:3 mass ratio stirring mixing, is then dried, and drying temperature is 20 DEG C, and water content is 10% after drying, bag
Dress, obtains final product.
The carrier is prepared according to following technique:
By zeolite and kaolinite according to 2:1 mass ratio puts into pulverization process in pulverizer, is then ground to the powder of 100 mesh
End;By above-mentioned powder, starch and shitosan according to 5:3:2 mass ratio is put into agitator, 1000rpm stirring 10min, obtains
To mixed material, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for mixed material matter
The concentration of amount 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 2mm;Particle is done in 80 DEG C of baking oven
Dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature,
Obtain final product.
Embodiment 2
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:
By xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173、
Micrococcus luteus (Micrococcus luteus) ATCC 49442, coral promise cassette bacterium (Nocardia coralline)
ACCC 40100, cellulomonas cartae (Cellulomonas sp) CGMCC No.2788 respectively according to cellar culture concentration be 1 ×
108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5:3:3:2 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent with
Carrier is according to 3:5 mass ratio stirring mixing, is then dried, and drying temperature is 20 DEG C, and water content is 15% after drying, bag
Dress, obtains final product.
The carrier is prepared according to following technique:
By zeolite and kaolinite according to 3:2 mass ratio puts into pulverization process in pulverizer, is then ground to the powder of 100 mesh
End;By above-mentioned powder, starch and shitosan according to 3:2:1 mass ratio is put into agitator, 1000rpm stirring 10min, obtains
To mixed material, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for mixed material matter
The concentration of amount 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 1mm;Particle is done in 80 DEG C of baking oven
Dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature,
Obtain final product.
Embodiment 3
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:
By xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173、
Micrococcus luteus (Micrococcus luteus) ATCC 49442, coral promise cassette bacterium (Nocardia coralline)
ACCC 40100, cellulomonas cartae (Cellulomonas sp) CGMCC No.2788 respectively according to cellar culture concentration be 1 ×
108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5:5:3:3 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent with
Carrier is according to 1:1 mass ratio stirring mixing, is then dried, and drying temperature is 20 DEG C, and water content is 125% after drying,
Packaging, obtains final product.
The carrier is prepared according to following technique:
By zeolite and kaolinite according to 1:1 mass ratio puts into pulverization process in pulverizer, is then ground to the powder of 100 mesh
End;By above-mentioned powder, starch and shitosan according to 3:2:2 mass ratio is put into agitator, 1000rpm stirring 10min, obtains
To mixed material, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for mixed material matter
The concentration of amount 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 1mm;Particle is done in 80 DEG C of baking oven
Dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature,
Obtain final product.
Embodiment 4
The compliance test result of biological agent of the present invention:
Take the gourmet powder fermenting waste mother liquor by the treatment of the operations such as precipitation of the abundant rich workshop in Inner Mongol(COD is 954mg/L, ammonia
Nitrogen 119mg/L, sulfide 37mg/L);
Handling process is:2 grams of microorganism formulation is added every time by every cubic metre of liquid, is added daily 1 time, continuously add 5 days, then
3 days are stood, finally filtering discharge.
COD, ammonia nitrogen, sulfide data are measured by sampling;And control group is set, the compatibility effect of each bacterial strain in detection microbial inoculum
Really:Control group 1:Without xylose oxidation and bacillus, remaining is with embodiment 1;Control group 2:Without micrococcus luteus, remaining is with real
Apply example 1;Control group 3:Without coral promise cassette bacterium, remaining is with embodiment 1;Control group 4:Without cellulomonas cartae, its
Remaining same embodiment 1.Specific testing result is shown in Table 1:
Table 1
Embodiment 1(mg/L) | Control group 1(mg/L) | Control group 2(mg/L) | Control group 3(mg/L) | Control group 4(mg/L) | |
COD | 5.8 | 30.6 | 66.4 | 41.6 | 59.8 |
NH3-N | 2.1 | 8.1 | 13.7 | 9.4 | 6.1 |
Sulfide | 1.2 | 2.9 | 5.9 | 4.1 | 3.7 |
Conclusion:Mushroom reasonable compatibility in biological agent of the invention, concertedness is strong, and usage amount is few, can effectively remove fermentation
COD, ammonia nitrogen and sulfide in waste liquid.
Embodiment 5
Test group:Biological agent prepared by the embodiment of the present invention 1;
Control group:Prepared by patent of invention technology " a kind of biological agent of the treatment gourmet powder waste water " embodiment 1 before applicant
Biological agent.
The indexs such as the density and sludge yield of biological agent are have detected, 2 are specifically shown in Table:
Table 2
Index | Density g/ml | 10d sludge yields g/L | 20d sludge yields g/L |
Test group | 1.03 | 3.2 | 9.8 |
Control group | 1.36 | 8.1 | 21.6 |
Conclusion:Biological agent density of the present invention and liquid phase are worked as, and can be suspended in liquid, it is to avoid deposition wadding collection to bottom of pond,
Produce substantial amounts of sludge, and hardness is bigger, non-breakable, long service life.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, the present invention not
It is limited to above example, there can also be many deformations.One of ordinary skill in the art can be direct from present disclosure
The all deformations derived or associate, are considered as protection scope of the present invention.
Claims (6)
1. the biological agent of gourmet powder fermenting mother liquor is processed, and it is prepared as follows and obtains:
Xylose oxidation and bacillus, micrococcus luteus, coral promise cassette bacterium and cellulomonas cartae is dense according to cellar culture respectively
Spend is 1 × 108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5-7:3-5:2-3:The volume ratio of 2-3 is mixed to get liquid bacteria
Agent;By liquid bacterial agent and carrier according to 2-3:The mass ratio stirring mixing of 3-5, is then dried, and packs, and obtains final product.
2. biological agent according to claim 1, it is characterised in that the xylose oxidation and bacillus are xylose oxidation and bacillus
(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173.
3. biological agent according to claim 1, it is characterised in that the micrococcus luteus is micrococcus luteus
(Micrococcus luteus)ATCC 49442。
4. biological agent according to claim 1, it is characterised in that the coral promise cassette bacterium is coral promise cassette
Bacterium (Nocardia coralline) ACCC 40100.
5. biological agent according to claim 1, it is characterised in that the cellulomonas cartae is cellulomonas cartae
(Cellulomonas sp) CGMCC No.2788。
6. the biological agent according to claim 1-5 is allowed to, it is characterised in that the carrier is prepared according to following technique
And obtain:By zeolite and kaolinite according to 2-3:The mass ratio of 1-2 puts into pulverization process in pulverizer, is then ground to 100 mesh
Powder;By above-mentioned powder, starch and shitosan according to 3-5:2-3:The mass ratio of 1-2 is put into agitator, and 1000rpm is stirred
10min is mixed, mixed material is obtained, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for
The concentration of mixed material quality 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 1-2mm;By particle in 80
DEG C baking oven in dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, from
Room temperature so is cooled to, is obtained final product.
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Cited By (2)
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CN110102177A (en) * | 2019-04-09 | 2019-08-09 | 卢松 | A kind of technique purifying slurry-spraying pelletizing flue gas |
CN110499262A (en) * | 2018-05-17 | 2019-11-26 | 卢松 | Purification absorbs the compound of slurry-spraying pelletizing flue gas |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN110499262A (en) * | 2018-05-17 | 2019-11-26 | 卢松 | Purification absorbs the compound of slurry-spraying pelletizing flue gas |
CN110102177A (en) * | 2019-04-09 | 2019-08-09 | 卢松 | A kind of technique purifying slurry-spraying pelletizing flue gas |
CN110102177B (en) * | 2019-04-09 | 2021-11-26 | 内蒙古阜丰生物科技有限公司 | Process for purifying flue gas generated in guniting granulation |
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