CN106929453A - Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof - Google Patents

Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof Download PDF

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CN106929453A
CN106929453A CN201710233087.0A CN201710233087A CN106929453A CN 106929453 A CN106929453 A CN 106929453A CN 201710233087 A CN201710233087 A CN 201710233087A CN 106929453 A CN106929453 A CN 106929453A
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biological agent
bacterium
mass ratio
micrococcus luteus
bacillus
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CN106929453B (en
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许传娟
刘海涛
丁兆堂
徐淑伟
高启超
杜鹏
高雷
王文强
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INNER MONGLIA FUFENG BIOLOGICAL TECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00Biological treatment of water, waste water, or sewage
    • C02F3/34Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier
    • CCHEMISTRY; METALLURGY
    • C02TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02FTREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/32Nature of the water, waste water, sewage or sludge to be treated from the food or foodstuff industry, e.g. brewery waste waters

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
  • Inorganic Chemistry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Hydrology & Water Resources (AREA)
  • Environmental & Geological Engineering (AREA)
  • Water Supply & Treatment (AREA)
  • Molecular Biology (AREA)
  • Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
  • Biological Treatment Of Waste Water (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The invention belongs to microbial technology field, the biological agent for the treatment of gourmet powder fermenting mother liquor is disclosed, it is prepared as follows and obtains:It is respectively 1 × 10 according to cellar culture concentration by xylose oxidation and bacillus, micrococcus luteus, coral promise cassette bacterium and cellulomonas cartae8The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 57:3‑5:2‑3:23 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent and carrier according to 23:35 mass ratio stirring mixing, is then dried, and packs, and obtains final product.Biological agent of the present invention only contains four kinds of bacterial strains, and reasonable compatibility, symbiosis is coordinated, mutually not antagonism, and long service life, sludge yield is few.

Description

Process biological agent of gourmet powder fermenting mother liquor and preparation method thereof
Technical field
The invention belongs to microbial technology field, and in particular to the biological agent for the treatment of gourmet powder fermenting mother liquor and its preparation side Method.
Background technology
Monosodium glutamate is a kind of food freshener being widely used, and fermentation method production monosodium glutamate will produce substantial amounts of high-concentration waste liquid, Monosodium glutamate waste liquid COD reaches 20-80g/L, and suspension, sulfate radical and ammonia-nitrogen content are high, and pH value is low, and intractability greatly, is processed into This high therefore many glutamate production producer fails effectively to process monosodium glutamate waste liquid, and pole is brought to environment and social development Big harm, monosodium glutamate industry production waste liquid pollution on the environment problem is on the rise, and the improvement of monosodium glutamate waste liquid has become Restrict the great difficult problem of glutamate production enterprise development.The main source of monosodium glutamate waste liquid is:It is useless after the extracted glutamic acid of zymotic fluid Mother liquor or ion exchange tail washings;Various equipment in production process (tank of sizing mixing, liquefied pot, saccharifying tank, fermentation tank, extractor, in With the tank etc. for decolourizing) scrub raffinate;Ion exchange resin is washed and regeneration liquid waste;Liquefaction is each to the fermentation etc. that is saccharified, is saccharified to The cooling water in stage;Various condensed waters (technique such as liquefaction, saccharification, concentration).
Current Wastewater treating method mainly has physical method, chemical method and biological method.Bioremediation is Administer the effective ways of industrial pollution water body.It is, using the effect of microorganism, to be decomposed compound.Due to biological treatment Method is more much lower than physics, chemical method cost, and non-secondary pollution, and microorganism has stronger variability and adaptation again Property, therefore it has turned into ideal method.Patent of invention technology " the biological system of a kind for the treatment of gourmet powder waste water before applicant Agent ", it uses microbial bacterial agent to process waste water, and COD, ammonia nitrogen, SS removal effects are good, but there is bacterial strain species in microbial inoculum Excessively, the possibility of bacterial strain pollution is increased, relative difficulty is cultivated, and microbial inoculum usage amount is bigger than normal, and sewage per ton needs plus 50g Microbial inoculum, microbial inoculum easily deposits to bottom of pond, causes wadding to collect, so as to produce substantial amounts of sludge, clears up relatively difficult.
The content of the invention
In order to overcome the defect of prior art, the invention provides the biological agent for the treatment of gourmet powder fermenting mother liquor.The present invention Additionally provide the preparation method of above-mentioned biological agent.
The present invention is achieved by the following technical solution:
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:By xylose oxidation and bacillus, M. luteus Bacterium, coral promise cassette bacterium and cellulomonas cartae are respectively 1 × 10 according to cellar culture concentration8The bacterium solution of cuf/g, will be above-mentioned Four kinds of bacterium solutions are according to 5-7:3-5:2-3:The volume ratio of 2-3 is mixed to get liquid bacterial agent;By liquid bacterial agent and carrier according to 2-3: The mass ratio stirring mixing of 3-5, is then dried, and packs, and obtains final product.
Preferably, the xylose oxidation and bacillus are xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173.
Preferably, the micrococcus luteus is micrococcus luteus (Micrococcus luteus) ATCC 49442.
Preferably, the coral promise cassette bacterium is coral promise cassette bacterium (Nocardia coralline) ACCC 40100。
Preferably, the cellulomonas cartae is cellulomonas cartae (Cellulomonas sp) CGMCC No.2788.
Preferably, the carrier is prepared according to following technique:By zeolite and kaolinite according to 2-3:The mass ratio of 1-2 Pulverization process in pulverizer is put into, the powder of 100 mesh is then ground to;By above-mentioned powder, starch and shitosan according to 3-5: 2-3:The mass ratio of 1-2 is put into agitator, 1000rpm stirring 10min, obtains mixed material, then with polystyrene microsphere According to 1:1 mass ratio is added in comminutor, is subsequently added into the polyvinyl alcohol for accounting for the concentration of mixed material quality 30% for 6wt% The aqueous solution, is made the particle that particle diameter is 1-2mm;Particle is dried into 30min in 80 DEG C of baking oven, then is put into and enter in sintering furnace Row sintering, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature, obtains final product.
The invention also discloses the preparation method of above-mentioned biological agent.
Strain of the present invention belongs to known bacterial strain, can be bought from databases such as CGMCC, ATCC and ACCC Arrive.The Amplification Culture of each strain of the invention is the cellar culture mode of this area, is not innovative point of the present invention, unknown herein State.
The beneficial effect that the present invention is obtained mainly includes but is not limited to the following aspects:
Biological agent of the present invention obtain using carrier and bacterium solution granulation, and specific surface area is big, thalline adhesive force by force, density and water body phase When, can suspend with water body, it is to avoid preparation density is excessive to be deposited in that microbial profile that bottom of pond causes is uneven and influence is removed Dirty effect, moreover it is possible to reduce the yield of sludge, is conducive to the removal of the pollutant such as COD and ammonia nitrogen in waste liquid;In order to reduce to list The dependence of one specific microbial inoculum, it is to avoid the loss that microbial inoculum pollution is caused occur, applicant develops various biological agents, mutually complementary Fill, it is ensured that the normal operation of sewage disposal;Biological agent of the present invention only contains four kinds of bacterial strains, and reasonable compatibility, symbiosis is coordinated, mutually not Antagonism, reduces cost, and operating procedure is also relatively easy.
Specific embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, below in conjunction with the application tool Body embodiment, the technical scheme to the application is clearly and completely described, it is clear that described embodiment is only this Shen Please a part of embodiment, rather than whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not having There is the every other embodiment made and being obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:
By xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173、 Micrococcus luteus (Micrococcus luteus) ATCC 49442, coral promise cassette bacterium (Nocardia coralline) ACCC 40100, cellulomonas cartae (Cellulomonas sp) CGMCC No.2788 respectively according to cellar culture concentration be 1 × 108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5:3:3:3 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent with Carrier is according to 2:3 mass ratio stirring mixing, is then dried, and drying temperature is 20 DEG C, and water content is 10% after drying, bag Dress, obtains final product.
The carrier is prepared according to following technique:
By zeolite and kaolinite according to 2:1 mass ratio puts into pulverization process in pulverizer, is then ground to the powder of 100 mesh End;By above-mentioned powder, starch and shitosan according to 5:3:2 mass ratio is put into agitator, 1000rpm stirring 10min, obtains To mixed material, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for mixed material matter The concentration of amount 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 2mm;Particle is done in 80 DEG C of baking oven Dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature, Obtain final product.
Embodiment 2
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:
By xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173、 Micrococcus luteus (Micrococcus luteus) ATCC 49442, coral promise cassette bacterium (Nocardia coralline) ACCC 40100, cellulomonas cartae (Cellulomonas sp) CGMCC No.2788 respectively according to cellar culture concentration be 1 × 108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5:3:3:2 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent with Carrier is according to 3:5 mass ratio stirring mixing, is then dried, and drying temperature is 20 DEG C, and water content is 15% after drying, bag Dress, obtains final product.
The carrier is prepared according to following technique:
By zeolite and kaolinite according to 3:2 mass ratio puts into pulverization process in pulverizer, is then ground to the powder of 100 mesh End;By above-mentioned powder, starch and shitosan according to 3:2:1 mass ratio is put into agitator, 1000rpm stirring 10min, obtains To mixed material, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for mixed material matter The concentration of amount 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 1mm;Particle is done in 80 DEG C of baking oven Dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature, Obtain final product.
Embodiment 3
The biological agent of gourmet powder fermenting mother liquor is processed, it is prepared as follows and obtains:
By xylose oxidation and bacillus(Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173、 Micrococcus luteus (Micrococcus luteus) ATCC 49442, coral promise cassette bacterium (Nocardia coralline) ACCC 40100, cellulomonas cartae (Cellulomonas sp) CGMCC No.2788 respectively according to cellar culture concentration be 1 × 108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5:5:3:3 volume ratio is mixed to get liquid bacterial agent;By liquid bacterial agent with Carrier is according to 1:1 mass ratio stirring mixing, is then dried, and drying temperature is 20 DEG C, and water content is 125% after drying, Packaging, obtains final product.
The carrier is prepared according to following technique:
By zeolite and kaolinite according to 1:1 mass ratio puts into pulverization process in pulverizer, is then ground to the powder of 100 mesh End;By above-mentioned powder, starch and shitosan according to 3:2:2 mass ratio is put into agitator, 1000rpm stirring 10min, obtains To mixed material, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for mixed material matter The concentration of amount 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 1mm;Particle is done in 80 DEG C of baking oven Dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, and naturally cools to room temperature, Obtain final product.
Embodiment 4
The compliance test result of biological agent of the present invention:
Take the gourmet powder fermenting waste mother liquor by the treatment of the operations such as precipitation of the abundant rich workshop in Inner Mongol(COD is 954mg/L, ammonia Nitrogen 119mg/L, sulfide 37mg/L);
Handling process is:2 grams of microorganism formulation is added every time by every cubic metre of liquid, is added daily 1 time, continuously add 5 days, then 3 days are stood, finally filtering discharge.
COD, ammonia nitrogen, sulfide data are measured by sampling;And control group is set, the compatibility effect of each bacterial strain in detection microbial inoculum Really:Control group 1:Without xylose oxidation and bacillus, remaining is with embodiment 1;Control group 2:Without micrococcus luteus, remaining is with real Apply example 1;Control group 3:Without coral promise cassette bacterium, remaining is with embodiment 1;Control group 4:Without cellulomonas cartae, its Remaining same embodiment 1.Specific testing result is shown in Table 1:
Table 1
Embodiment 1(mg/L) Control group 1(mg/L) Control group 2(mg/L) Control group 3(mg/L) Control group 4(mg/L)
COD 5.8 30.6 66.4 41.6 59.8
NH3-N 2.1 8.1 13.7 9.4 6.1
Sulfide 1.2 2.9 5.9 4.1 3.7
Conclusion:Mushroom reasonable compatibility in biological agent of the invention, concertedness is strong, and usage amount is few, can effectively remove fermentation COD, ammonia nitrogen and sulfide in waste liquid.
Embodiment 5
Test group:Biological agent prepared by the embodiment of the present invention 1;
Control group:Prepared by patent of invention technology " a kind of biological agent of the treatment gourmet powder waste water " embodiment 1 before applicant Biological agent.
The indexs such as the density and sludge yield of biological agent are have detected, 2 are specifically shown in Table:
Table 2
Index Density g/ml 10d sludge yields g/L 20d sludge yields g/L
Test group 1.03 3.2 9.8
Control group 1.36 8.1 21.6
Conclusion:Biological agent density of the present invention and liquid phase are worked as, and can be suspended in liquid, it is to avoid deposition wadding collection to bottom of pond, Produce substantial amounts of sludge, and hardness is bigger, non-breakable, long service life.
Finally, in addition it is also necessary to it is noted that listed above is only several specific embodiments of the invention.Obviously, the present invention not It is limited to above example, there can also be many deformations.One of ordinary skill in the art can be direct from present disclosure The all deformations derived or associate, are considered as protection scope of the present invention.

Claims (6)

1. the biological agent of gourmet powder fermenting mother liquor is processed, and it is prepared as follows and obtains:
Xylose oxidation and bacillus, micrococcus luteus, coral promise cassette bacterium and cellulomonas cartae is dense according to cellar culture respectively Spend is 1 × 108The bacterium solution of cuf/g, by above-mentioned four kinds of bacterium solutions according to 5-7:3-5:2-3:The volume ratio of 2-3 is mixed to get liquid bacteria Agent;By liquid bacterial agent and carrier according to 2-3:The mass ratio stirring mixing of 3-5, is then dried, and packs, and obtains final product.
2. biological agent according to claim 1, it is characterised in that the xylose oxidation and bacillus are xylose oxidation and bacillus (Achromobacter xylosoxidans subsp.denitrificans)ATCC 15173.
3. biological agent according to claim 1, it is characterised in that the micrococcus luteus is micrococcus luteus (Micrococcus luteus)ATCC 49442。
4. biological agent according to claim 1, it is characterised in that the coral promise cassette bacterium is coral promise cassette Bacterium (Nocardia coralline) ACCC 40100.
5. biological agent according to claim 1, it is characterised in that the cellulomonas cartae is cellulomonas cartae (Cellulomonas sp) CGMCC No.2788。
6. the biological agent according to claim 1-5 is allowed to, it is characterised in that the carrier is prepared according to following technique And obtain:By zeolite and kaolinite according to 2-3:The mass ratio of 1-2 puts into pulverization process in pulverizer, is then ground to 100 mesh Powder;By above-mentioned powder, starch and shitosan according to 3-5:2-3:The mass ratio of 1-2 is put into agitator, and 1000rpm is stirred 10min is mixed, mixed material is obtained, then with polystyrene microsphere according to 1:1 mass ratio is added in comminutor, is subsequently added into and accounts for The concentration of mixed material quality 30% is the polyvinyl alcohol water solution of 6wt%, is made the particle that particle diameter is 1-2mm;By particle in 80 DEG C baking oven in dry 30min, then put into and be sintered in sintering furnace, 700 DEG C of sintering temperature is incubated 20min, takes out, from Room temperature so is cooled to, is obtained final product.
CN201710233087.0A 2017-04-11 2017-04-11 Biological agent for treating monosodium glutamate fermentation mother liquor and preparation method thereof Active CN106929453B (en)

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CN110102177A (en) * 2019-04-09 2019-08-09 卢松 A kind of technique purifying slurry-spraying pelletizing flue gas
CN110499262A (en) * 2018-05-17 2019-11-26 卢松 Purification absorbs the compound of slurry-spraying pelletizing flue gas

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110499262A (en) * 2018-05-17 2019-11-26 卢松 Purification absorbs the compound of slurry-spraying pelletizing flue gas
CN110102177A (en) * 2019-04-09 2019-08-09 卢松 A kind of technique purifying slurry-spraying pelletizing flue gas
CN110102177B (en) * 2019-04-09 2021-11-26 内蒙古阜丰生物科技有限公司 Process for purifying flue gas generated in guniting granulation

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