CN106922531A - A kind of banana Multiple Buds go out the screening technique of root rate condition of culture high - Google Patents
A kind of banana Multiple Buds go out the screening technique of root rate condition of culture high Download PDFInfo
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- CN106922531A CN106922531A CN201710171858.8A CN201710171858A CN106922531A CN 106922531 A CN106922531 A CN 106922531A CN 201710171858 A CN201710171858 A CN 201710171858A CN 106922531 A CN106922531 A CN 106922531A
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- culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The present invention provides the screening technique that a kind of banana Multiple Buds go out root rate condition of culture high, strengthen the physiological effect of the wavelength to Banana Tissue cultivating seedling of light source by using the adjustment test of unitary variant method, it is determined that most suitable enhancing optical source wavelength, and the influence according to light sources with different wavelengths to tissue culture seedlings of bananas, determine that light sources with different wavelengths goes out combination, and by keeping the enhancing constant reverse determination basal medium of light source, introduce tissue-cultured seedling simultaneously actually goes out root rate as screening criteria, is more suitable for commercial plant metaplasia product.
Description
Technical field
Go out root rate culture bar high the invention belongs to Banana Tissue culture technique field, more particularly to a kind of banana Multiple Buds
The screening technique of part.
Background technology
Banana（Scientific name：Musa nana Lour.）Musaceae banana, refers to its fruit again.Torrid areas plants extensively
Training is edible.Banana flavor is fragrant, rich in nutrition, can harvest throughout the year, also paid attention to very much in Temperate Region in China.Plant is large-scale draft, from root
Shape stem is sent, and the false bar of (10~20 chi) high 3~6 meters is formed by leaf sheath bottom；Leaf Long Circle to ellipse, have up to 3
~3.5 meters (10~11.5 chi), (26 cun) wide 65 centimeters, 10~20 pieces of fasciation stem tops.Spike is sagging[1], by false bar top
End is extracted out, spends majority, faint yellow；Infructescence is curved to hang down, as a result 10~20 string, about 50~150.It is withered after plant result, by root shape
The sucker that stem length goes out continues to breed, and each stock can live for many years.Originate in the Asia southeast：The ground such as Taiwan, Hainan, Guangdong, Guangxi
Qu Junyou is cultivated.Current banana is a kind of very popular fruit, is also the main food of some areas, and possessing very big market needs
The amount of asking, the research that quick breeding is carried out to banana has certain necessity, can not only solve huge market demand, also can
Excellent banana variety is preserved to a certain extent, and tissue cultures are one of banana rapid propagation methods, but existing perfume (or spice)
The technology of Banana Growth is rarely adjusted in any of several broadleaf plants culture medium by strengthening light source.
Cerium is periodic system Ι Ι Ι races subgroup lanthanide series, a kind of rare earth element.Atomic number 58.Stable isotope：136、
138、140、142.Grey metal, there is malleability.Density：Quadratic crystal 6.9, cubic crystal 6.7.799 DEG C of fusing point, boiling point 3426
℃.Cerium is a kind of silver-gray active metal, and powder easy spontaneous combustion in atmosphere is soluble in acid.The title of cerium derives from asteroid
The English name of ceres.Content of the cerium in the earth's crust about 0.0046%, is abundance highest in rare earth element.
The content of the invention
Above mentioned problem is had based on prior art, the present invention provides a kind of banana Multiple Buds and goes out root rate condition of culture high
Screening technique, the physiological effect of the wavelength to Banana Tissue cultivating seedling of light source is strengthened by using the adjustment test of unitary variant method,
It is determined that most suitable enhancing optical source wavelength, and the influence according to light sources with different wavelengths to tissue culture seedlings of bananas, determine that light sources with different wavelengths goes out
Combination, and by keeping enhancing light source constant reverse determination basal medium, while introducing tissue-cultured seedling actually goes out root rate conduct
Screening criteria, is more suitable for commercial plant metaplasia product.
A kind of banana Multiple Buds go out the screening technique of root rate condition of culture high, and it is comprised the following steps：
Step S10 determines preliminary screening scope, and data of literatures is consulted in retrieval, contrasts other plant species tissue culture rooting process
Middle addition strengthens light source category and determines the preliminary screening scope of optical source wavelength；
Step S20 selects explant, selection health, the banana Multiple Buds that growing environment is consistent and growth conditions are similar, by clump
Sprout and be divided into simple bud；
Step S30 control cultures, the culture medium based on 1/2MS culture mediums, the volume fraction of aspirin solution be for
0.03%、NAA（Methyl α-naphthyl acetate）Concentration be 0.15mg/L, PP333The concentration of (paclobutrazol) is that 1 mg/L, the concentration of lanthanum nitrate are
3g/L, the concentration of vitamin B12 is 0.3mg/L, and the concentration of agar powder is 10g/L, the concentration of white granulated sugar is 7g/L, is configured to survey
Culture medium on probation, is inoculated with same amount of banana Multiple Buds respectively, and carries out conventional root tissues culture as a control group, in routine
Used as experimental group, control group all sets multiple parallel condition different-waveband enhancing enhancing light source with experimental group on the basis of culture
Group；
Step S40 record detection parameters, culture counts the survival banana bud that goes out root in each experimental group and control group after 20 days
Quantity, and calculating effectively goes out root rate；
Step S50 increments are calculated, and respectively take out the banana bud in each parallel laboratory test group of same light source wavelength, use clear water
Rinse well, cut out root after drying surface moisture, and root is weighed, record weight；
Step S60 calculates assessment factor, will effectively go out root rate and be multiplied by ten points one times of root weight to draw assessment factor, and contrast is different
The assessment factor of wavelength gradient, picks out the numerical value maximum corresponding smooth source category of assessment factor and optical source wavelength；
Step S70 reduces screening scope, and step S10- is repeated using the enhancing optical source wavelength scope picked out in step S60
S60 reduces screening scope or determines optimum wavelength scope；
Step S80 reversely screens basal medium, the different basis cultures of optimum wavelength scope collocation determined using step S70
Base, repeat step S40 and step S50, and using the computational methods calculating assessment factor of step S60, it is determined that most suitable basis culture
Base；
Step S90 different wave length combined sortings, determine that different wave length is joined to the physiology of banana adventitious buds proliferation by single factor
Number influence, adjustment is best suitable for wavelength combination.
Preferably, each experimental group or control group set 5-15 parallel group in described step S30.
Preferably, the number of times repeated in described step S70 is 2-3 times.
Preferably, different basal mediums includes 1/2MS culture mediums, MS culture mediums, N6 cultures in described step S80
Base, B5 medium, Nitsh culture mediums, White culture mediums and Miller culture mediums.
Specific embodiment
With reference to specific embodiment, the invention will be further described.
A kind of banana Multiple Buds go out the screening technique of root rate condition of culture high, and it is comprised the following steps：
Step S10 determines preliminary screening scope, and data of literatures is consulted in retrieval, contrasts other plant species tissue culture rooting process
Middle addition strengthens light source category and determines the preliminary screening scope of optical source wavelength；
Step S20 selects explant, selection health, the banana Multiple Buds that growing environment is consistent and growth conditions are similar, by clump
Sprout and be divided into simple bud；
Step S30 control cultures, the culture medium based on 1/2MS culture mediums, the volume fraction of aspirin solution be for
0.03%、NAA（Methyl α-naphthyl acetate）Concentration be 0.15mg/L, PP333The concentration of (paclobutrazol) is that 1 mg/L, the concentration of lanthanum nitrate are
3g/L, the concentration of vitamin B12 is 0.3mg/L, and the concentration of agar powder is 10g/L, the concentration of white granulated sugar is 7g/L, is configured to survey
Culture medium on probation, is inoculated with same amount of banana Multiple Buds respectively, and carries out conventional root tissues culture as a control group, in routine
Used as experimental group, control group all sets multiple parallel condition different-waveband enhancing enhancing light source with experimental group on the basis of culture
Group；
Step S40 record detection parameters, culture counts the survival banana bud that goes out root in each experimental group and control group after 20 days
Quantity, and calculating effectively goes out root rate；
Step S50 increments are calculated, and respectively take out the banana bud in each parallel laboratory test group of same light source wavelength, use clear water
Rinse well, cut out root after drying surface moisture, and root is weighed, record weight；
Step S60 calculates assessment factor, will effectively go out root rate and be multiplied by ten points one times of root weight to draw assessment factor, and contrast is different
The assessment factor of wavelength gradient, picks out the numerical value maximum corresponding smooth source category of assessment factor and optical source wavelength；
Step S70 reduces screening scope, and step S10- is repeated using the enhancing optical source wavelength scope picked out in step S60
S60 reduces screening scope or determines optimum wavelength scope；
Step S80 reversely screens basal medium, the different basis cultures of optimum wavelength scope collocation determined using step S70
Base, repeat step S40 and step S50, and using the computational methods calculating assessment factor of step S60, it is determined that most suitable basis culture
Base；
Step S90 different wave length combined sortings, determine that different wave length is joined to the physiology of banana adventitious buds proliferation by single factor
Number influence, adjustment is best suitable for wavelength combination.
As the presently preferred embodiments, each experimental group or control group set 10 parallel groups in described step S30.
As the presently preferred embodiments, the number of times repeated in described step S70 is 3 times.
As the presently preferred embodiments, different basal mediums includes that 1/2MS culture mediums, MS are cultivated in described step S80
Base, N6 culture mediums, B5 medium, Nitsh culture mediums, White culture mediums and Miller culture mediums.
Being defined below enhancing light source by screening has the quality for actually going out root rate and root higher：Use natural light, indigo plant
Light strengthens light source and feux rouges enhancing light source irradiation Multiple Buds, and red light source is the feux rouges that wave band is 650nm-700nm, blue-light source
It is that wave band is the blue light of 450nm-470nm, light application time is illumination 16 hours/dark 8 hours, feux rouges 80%, blue light 20%.
Claims (4)
1. a kind of banana Multiple Buds go out the screening technique of root rate condition of culture high, it is characterised in that it is comprised the following steps：
Step S10 determines preliminary screening scope, and data of literatures is consulted in retrieval, contrasts other plant species tissue culture rooting process
Middle addition strengthens light source category and determines the preliminary screening scope of optical source wavelength；
Step S20 selects explant, selection health, the banana Multiple Buds that growing environment is consistent and growth conditions are similar, by clump
Sprout and be divided into simple bud；
Step S30 control cultures, the culture medium based on 1/2MS culture mediums, the volume fraction of aspirin solution be for
0.03%、NAA（Methyl α-naphthyl acetate）Concentration be 0.15mg/L, PP333The concentration of (paclobutrazol) is that 1 mg/L, the concentration of lanthanum nitrate are
3g/L, the concentration of vitamin B12 is 0.3mg/L, and the concentration of agar powder is 10g/L, the concentration of white granulated sugar is 7g/L, is configured to survey
Culture medium on probation, is inoculated with same amount of banana Multiple Buds respectively, and carries out conventional root tissues culture as a control group, in routine
Used as experimental group, control group all sets multiple parallel condition different-waveband enhancing enhancing light source with experimental group on the basis of culture
Group；
Step S40 record detection parameters, culture counts the survival banana bud that goes out root in each experimental group and control group after 20 days
Quantity, and calculating effectively goes out root rate；
Step S50 increments are calculated, and respectively take out the banana bud in each parallel laboratory test group of same light source wavelength, use clear water
Rinse well, cut out root after drying surface moisture, and root is weighed, record weight；
Step S60 calculates assessment factor, will effectively go out root rate and be multiplied by ten points one times of root weight to draw assessment factor, and contrast is different
The assessment factor of wavelength gradient, picks out the numerical value maximum corresponding smooth source category of assessment factor and optical source wavelength；
Step S70 reduces screening scope, and step S10- is repeated using the enhancing optical source wavelength scope picked out in step S60
S60 reduces screening scope or determines optimum wavelength scope；
Step S80 reversely screens basal medium, the different basis cultures of optimum wavelength scope collocation determined using step S70
Base, repeat step S40 and step S50, and using the computational methods calculating assessment factor of step S60, it is determined that most suitable basis culture
Base；
Step S90 different wave length combined sortings, determine that different wave length is joined to the physiology of banana adventitious buds proliferation by single factor
Number influence, adjustment is best suitable for wavelength combination.
2. a kind of banana Multiple Buds according to claim 1 go out the screening technique of root rate condition of culture high, and its feature exists
In each experimental group or control group set 5-15 parallel group in described step S30.
3. a kind of banana Multiple Buds according to claim 1 go out the screening technique of root rate condition of culture high, and its feature exists
In the number of times repeated in described step S70 is 2-3 times.
4. a kind of banana Multiple Buds according to claim 1 go out the screening technique of root rate condition of culture high, and its feature exists
In, in described step S80 different basal mediums include 1/2MS culture mediums, MS culture mediums, N6 culture mediums, B5 medium,
Nitsh culture mediums, White culture mediums and Miller culture mediums.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710171858.8A CN106922531A (en) | 2017-03-22 | 2017-03-22 | A kind of banana Multiple Buds go out the screening technique of root rate condition of culture high |
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| CN201710171858.8A CN106922531A (en) | 2017-03-22 | 2017-03-22 | A kind of banana Multiple Buds go out the screening technique of root rate condition of culture high |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109302957A (en) * | 2018-11-13 | 2019-02-05 | 广西壮族自治区农业科学院 | A method of passing through medicament adjusting preventing and controlling banana fusarium wilt |
-
2017
- 2017-03-22 CN CN201710171858.8A patent/CN106922531A/en active Pending
Non-Patent Citations (8)
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| 刘清涌等: "《养兰金典：兰花栽培私家秘决》", 31 October 2011, 福建科学技术出版社 * |
| 博观堂: "《家庭养花事典》", 31 August 2009, 福建科学技术出版社 * |
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| 杨秀坚等: "不同植物生长调节剂对香蕉组织培养的影响", 《广东农业科学》 * |
| 王小敏: "香蕉试管苗的繁殖及其变异的研究", 《中国优秀博硕士学位论文全文数据库（硕士） 农业科技辑》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109302957A (en) * | 2018-11-13 | 2019-02-05 | 广西壮族自治区农业科学院 | A method of passing through medicament adjusting preventing and controlling banana fusarium wilt |
| CN109302957B (en) * | 2018-11-13 | 2020-08-11 | 广西壮族自治区农业科学院 | Method for preventing and controlling banana wilt through drug regulation |
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Application publication date: 20170707 |