CN106912376A - The asexual reproduction method of asparagus - Google Patents
The asexual reproduction method of asparagus Download PDFInfo
- Publication number
- CN106912376A CN106912376A CN201510983558.0A CN201510983558A CN106912376A CN 106912376 A CN106912376 A CN 106912376A CN 201510983558 A CN201510983558 A CN 201510983558A CN 106912376 A CN106912376 A CN 106912376A
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- CN
- China
- Prior art keywords
- asparagus
- sterilization
- frond
- seawater flushing
- reproduction method
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of asexual reproduction method of asparagus, step is as follows：（1）Select the immature asparagus of health, repeatedly scrub, again with sterilization seawater flushing, the protozoan and the miscellaneous algae that grows nonparasitically upon another plant of frond surface parasitic are removed, after sterilization cultivation in sea water one week, scrub repeatedly, untill microscopy frond surface is without miscellaneous algae, it is soaked in 10 minutes in 1.5% KI solution, then with sterilization seawater flushing 3 times, with 70% alcohol-pickled 10s, then with sterilization seawater flushing 3 times；（2）The asparagus frond knife blade that will be handled well is cut into the segment of 2-4mm, cultivates in the PES nutrient solutions containing 0.25mg/mL6-BA, dark culturing 7 days；（3）Changing condition of culture is：17 DEG C of temperature, the μ E m of intensity of illumination 10 ± 2−2•s−1, the photoperiod is 16h:8h(Light:Dark).Operation is simple for asexual reproduction method of the invention, and induction duration is short, is also that the production production of hybrid seeds and expansion are numerous, there is provided support for the biological engineering of asparagus and nursery provide technical method.
Description
Technical field
The present invention relates to a kind of asexual reproduction method of asparagus.
Background technology
Asparagus（Gracilaria lemaneiformis）It is Gracilaria（Gracilaria）, it is under the jurisdiction of true Rhodophyceae
Florideae, Gigartinales Gigartinales, fragrant plant mentioned in ancient texts section Gracilariaceae, are distributed in China coast various regions.Dragon
Palpus dish is in cylindrical, linear branch.Branch alternate, wilfully, its base portion is slightly hung.Every plant of base portion is patelliform holdfast, major branch
It is thick compared with branch.The plump succulence of algae branch.Color is reddish brown, puce, sometimes with green or Huang, crineous is changed into after doing, and algae branch shrinks.Dragon
Palpus dish is important economical alga, and its frond agar content is up to more than 30%, is the important source material for manufacturing agar-agar and agarose
One of, currently as bacterium, the culture medium of microorganism, agar-agar is widely used in work, agriculture, pharmaceutical sector.With the demand to agar-agar
Amount is continuously increased, and the raw material supply of asparagus is much not enough, and due to the supply problem of seed, cannot still be fully solved,
Therefore the development propagated artificially is limited.The report of the current Study on tissue culture on asparagus is less.
The content of the invention
The present invention is intended to provide a kind of asexual reproduction method of asparagus, solves the problems, such as that seed is insufficient.
A kind of asexual reproduction method of asparagus, step is as follows：
（1）The immature asparagus of health is selected, is repeatedly scrubbed, then with sterilization seawater flushing, remove frond surface parasitic
Protozoan and the miscellaneous algae that grows nonparasitically upon another plant, with sterilization cultivation in sea water one week after, scrub repeatedly, untill microscopy frond surface is without miscellaneous algae,
It is soaked in 10 minutes in 1.5% KI solution, then with sterilization seawater flushing 3 times, with 70% alcohol-pickled 10 s, then with disappearing
Malicious seawater flushing 3 times；
（2）The asparagus frond knife blade that will be handled well is cut into the segment of 2-4 mm, and culture is containing 0.25mg/mL 6-
In the PES nutrient solutions of BA, dark culturing 7 days；
（3）Changing condition of culture is：17 DEG C of temperature, the μ E m of intensity of illumination 10 ± 2−2•s−1, the photoperiod is 16h:8h
(Light: Dark)。
Asexual reproduction method of the invention can induce the upright shoot of generation.Operation is simple, and induction duration is short, and can
Regeneration shoot is further cultured for, its regeneration shoot is induced, clone is formed.Using asparagus clone, can be with
Carry out indoor preserving seed and expand numerous, solidify the merit of selection, be that the biological engineering of asparagus and nursery provide technical side
Method, is also that the production production of hybrid seeds and expansion are numerous, there is provided support.
Specific embodiment
The present invention is described in further details with reference to specific embodiment.
A kind of asexual reproduction method of asparagus, step is as follows：
（1）The immature asparagus of health is selected, is repeatedly scrubbed, then with sterilization seawater flushing, remove frond surface parasitic
Protozoan and the miscellaneous algae that grows nonparasitically upon another plant, with sterilization cultivation in sea water one week after, scrub repeatedly, untill microscopy frond surface is without miscellaneous algae,
It is soaked in 10 minutes in 1.5% KI solution, then with sterilization seawater flushing 3 times, with 70% alcohol-pickled 10 s, then with disappearing
Malicious seawater flushing 3 times；
（2）The asparagus frond knife blade that will be handled well is cut into the segment of 2-4 mm, and culture is containing 0.25mg/mL 6-
In the PES nutrient solutions of BA, dark culturing 7 days；
（3）Changing condition of culture is：17 DEG C of temperature, the μ E m of intensity of illumination 10 ± 2−2•s−1, the photoperiod is 16h:8h
(Light: Dark)。
Culture is respectively cut off after 25 days there is regeneration shoot.
Claims (1)
1. a kind of asexual reproduction method of asparagus, it is characterised in that step is as follows：
（1）The immature asparagus of health is selected, is repeatedly scrubbed, then with sterilization seawater flushing, remove frond surface parasitic
Protozoan and the miscellaneous algae that grows nonparasitically upon another plant, with sterilization cultivation in sea water one week after, scrub repeatedly, untill microscopy frond surface is without miscellaneous algae,
It is soaked in 10 minutes in 1.5% KI solution, then with sterilization seawater flushing 3 times, with 70% alcohol-pickled 10 s, then with disappearing
Malicious seawater flushing 3 times；
（2）The asparagus frond knife blade that will be handled well is cut into the segment of 2-4 mm, and culture is containing 0.25mg/mL 6-
In the PES nutrient solutions of BA, dark culturing 7 days；
（3）Changing condition of culture is：17 DEG C of temperature, the μ E m of intensity of illumination 10 ± 2−2•s−1, the photoperiod is 16h:8h (Light:
Dark)。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510983558.0A CN106912376A (en) | 2015-12-24 | 2015-12-24 | The asexual reproduction method of asparagus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510983558.0A CN106912376A (en) | 2015-12-24 | 2015-12-24 | The asexual reproduction method of asparagus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106912376A true CN106912376A (en) | 2017-07-04 |
Family
ID=59456533
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510983558.0A Pending CN106912376A (en) | 2015-12-24 | 2015-12-24 | The asexual reproduction method of asparagus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106912376A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631128A (en) * | 2020-04-23 | 2020-09-08 | 莆田市正洋水产发展有限公司 | Method for removing miscellaneous algae in asparagus cultivation |
| CN113545288A (en) * | 2020-04-23 | 2021-10-26 | 莆田市正洋水产发展有限公司 | Method for shortening cultivation period of asparagus |
| CN113796304A (en) * | 2021-09-24 | 2021-12-17 | 福建省水产研究所（福建水产病害防治中心） | Rapid budding method for cutting asparagus seedlings |
-
2015
- 2015-12-24 CN CN201510983558.0A patent/CN106912376A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111631128A (en) * | 2020-04-23 | 2020-09-08 | 莆田市正洋水产发展有限公司 | Method for removing miscellaneous algae in asparagus cultivation |
| CN113545288A (en) * | 2020-04-23 | 2021-10-26 | 莆田市正洋水产发展有限公司 | Method for shortening cultivation period of asparagus |
| CN113796304A (en) * | 2021-09-24 | 2021-12-17 | 福建省水产研究所（福建水产病害防治中心） | Rapid budding method for cutting asparagus seedlings |
| CN116267578A (en) * | 2021-09-24 | 2023-06-23 | 福建省水产研究所（福建水产病害防治中心） | Rapid sprouting method for asparagus seedling cut segments and application of low-salt culture solution |
| CN116267578B (en) * | 2021-09-24 | 2024-09-03 | 福建省水产研究所（福建水产病害防治中心） | Rapid sprouting method for asparagus seedling cut segments and application of low-salt culture solution |
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Legal Events
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| PB01 | Publication | ||
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170704 |