CN106872238B - A kind of low cost automatic sample Preparation equipment - Google Patents
A kind of low cost automatic sample Preparation equipment Download PDFInfo
- Publication number
- CN106872238B CN106872238B CN201710255703.2A CN201710255703A CN106872238B CN 106872238 B CN106872238 B CN 106872238B CN 201710255703 A CN201710255703 A CN 201710255703A CN 106872238 B CN106872238 B CN 106872238B
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- Prior art keywords
- module
- cavity base
- upper cover
- sample preparation
- closed
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
Abstract
The invention discloses a kind of inexpensive automatic sample Preparation equipments; belong to molecular diagnostic techniques field; including closed module; the module can be used for the storage of volatile liquid in sample preparation procedure; it can also be used for the low-temperature protection of active material, it may also be used for enzyme reaction etc. needs thermostatically controlled processing.The closed module is made of heat-insulating heat-preserving material, sealing material, motor, transmission mechanism etc..Present invention introduces closed module NGS sample preparations can be made to realize that low cost is uninterrupted, full-automatic operation.
Description
Technical field
The invention belongs to molecular diagnostic techniques fields, and in particular to a kind of low cost automatic sample Preparation equipment.
Background technology
Sequencing has become the important means of accurate medicine, have begun gradually to be applied to now clinical etiological diagnosis and
On targeted therapy.Genome sequencing price, can be still huge due to sequencing data so that when detection although having declined very much
Between and cost still restrict it is clinically widely used.Sequencing data amount can be greatly reduced in the detection in DNA marks site, into
And testing cost and period are reduced, it is a kind of molecular diagnosis scheme that clinic generally uses.Sequenator has obtained extensively at present
Using the capture means for identifying site DNA fragmentation are also more and more abundant, this just further promotes the development of accurate medicine.
It is complicated that sample preparation procedure is sequenced, processing time is long, requires height to process step, identifies the sample preparation of site DNA fragmentation
Process is more complicated, the requirement higher to process step.Currently, there are no a universal sequencing clinical sample preparations to set in the market
Standby, the present invention is exactly a sequencing sample preparation apparatus for clinical application, and the present disclosure applies equally to the detections of mass spectrum class.
The sample preparation mode being sequenced at present mainly have complete artificial manual mode and by automation semi-artificial half from
Why dynamicization mode, be not carried out unattended full-automatic mode, can not mainly solve volatile reagent concentration and protect
Aerosol when holding and heating and volatilization problems.Current semi-automatic mode solution, be need to add it is volatile
When reagent, suspend automation equipment, manually add volatile reagent, then starts automation equipment；Also it is exactly, in routine
Volatile substances are stored in outside in method, are squeezed into using external pump, equipment volume can be caused huge in this way, pipeline is complicated,
Waste is serious, needs to clean；Or the mode of heating of conventional enzyme reaction is using integrated expensive PCR instrument, volume is big, valence
Lattice are high, waste one's talent on a petty job or do not realized automatically using artificial external PCR instrument heating.In addition, conventional active agent
It is stored on open cryogenic refrigerator, can make by the water condensation in air in active reservoir in freezing is kept
When must need active material, it is unable to ensure quality.If solved these problems to full-automation, additional infusion pump systems are needed
With integrated PCR equipment, it is low and expensive to implement system complex, reliability.
Invention content
In order to overcome, equipment manufacturing cost is expensive, bulky, mechanism in sequencing in the prior art or Mass Spectrometer Method sample device
Complexity, and condensed water and the variation of volatile substances concentration are will produce, the preparation of sample is influenced, and full-automation can not be solved
The deficiencies of, the present invention provides a kind of inexpensive automatic sample Preparation equipment, which introduces closed module, closed
Reservoir is added in module, can be used for storing volatile liquid, so that it is guaranteed that the concentration of volatile liquid；Add in closed module
Enter refrigerator, can be used for preserving active material and survive for a long time, while moisture condensation situation will not be generated in closed environment；Close
Heater is added in mold closing block, can be used for the reaction of constant temperature reagent.The closed modular structure is simple, light and handy, cheap, automatically
Control, can be formed together with existing oscillator module, Magneto separate module, the function module of scan module one it is inexpensive complete
Automate sample preparation device.
The technical solution adopted by the present invention to solve the technical problems is：
The lower part of a kind of low cost automatic sample Preparation equipment, including closed module, the closed module is cavity
Base construction, hinged upper cover above cavity base, upper cover lower surface is provided with phase with cavity base upper surface surrounding contact site
Mutually mating flexible seal device, cavity base are provided with motor outside, and motor connects upper cover in drive by transmission mechanism
The folding of lid, when that need not be moved in and out to substance in cavity, motor rotates clockwise, and transmission mechanism drives upper cover downlink,
Until flexible seal device touches cavity base upper end, and deformation is generated, realizes sealing effect, need to cavity inside substance
When moving in and out, motor rotates in an anti-clockwise direction, and upper cover is allowed fully to open, and pipetting head can successfully move in and out internal object
Matter.
Preferably, the cavity base is internally provided with reservoir, and reservoir makes its guarantor for storing volatile liquid
It holds that long-time density is constant, avoids the complexity, costliness, low reliability of external infusion pumping system.
After introducing cavity base does reservoir, required pipeline and pump, avoid when without using adding volatile reagent
The complexity of equipment is high with cost.
It introduces cavity base and stores volatile reagent, preparation flow can be carried out continuously, and need not stop preparing stream halfway
Journey adds volatile reagent.
Preferably, the cavity base is internally provided with refrigerator, and refrigerator freezes to cavity base inside, makes
Activity can be kept for a long time by obtaining when cavity base preserves active material, avoid condensing.
After introducing cavity base makees refrigerator, instead of open refrigeration device, avoids condensed water and reagent is kept to low temperature
Influence.
Preferably, be both provided with heating device inside the cavity base and upper cover, heating device to cavity base and
Upper cover is heated, and realizes the High Accuracy Constant Temperature control in enzyme reaction, upper cover and mating flexible seal device to cavity base into
Row sealing, preventing from evaporating in heating process carries out with aerosol cross contamination, without being placed in PCR instrument, so as to avoid
The rising of the occupancy and cost of the device space brought using integrated form PCR instrument.
The cavity base and upper cover of introducing all have heating function, and preparation flow can be carried out continuously, and need not be stopped halfway
Only preparation flow does enzyme reaction in device external.
The cavity base and upper cover of introducing all have heating function, do heating function without using integrated form PCR, avoid
The huge space and high expense that the PCR of preparation system is occupied.
It is an advantage of the invention that：Present invention introduces closed module realize multiple functions, be sealing function first, will store up
Liquid bath is set as the purity of the conditions warrant volatile liquid of sealing；Refrigeration cool-down function is followed by realized, ensures cavity base
Seat in substance activity and prevent from condensing；Again be realize heating function, can be realized in equipment low price, high-precision add
Thermal response.The full-automatic inexpensive sample preparation device of the present invention uses integrative collocation, is existed according to different sample preparation demands
In the liquor removing workstation that three-dimensional motion manipulator and accurate pipetting head are constituted, closed liquid storage module, airtight heating mould are organically combined
The function systems such as block, sealed refrigeration module, oscillation module, Magneto separate module, mistake proofing module, flap module are completed to DNA sample
Plasmid extraction, library create, the processing such as enzymatic reagent reaction, to complete the sample of whole process DNA detection on a machine
This preparation.Also, cavity base is suitable at present the consumptive materials such as general 96 orifice plate of standard, Reagent Tube, reagent trough on the market, is convenient for
It is mating with consumptive material progress used in middle-end client's kit, customize consumptive material without introducing.
Description of the drawings
Fig. 1 is the structural schematic diagram of closed modular system；
Fig. 2 is that closed module is placed in heater and PCR plate, and the structural schematic diagram of enzyme reaction is realized in PCR plate.
Specific implementation mode
Below in conjunction with attached drawing, the present invention is further illustrated：
Embodiment 1
As shown in Figure 1, a kind of low cost automatic sample Preparation equipment, including closed module, the closed module
Lower part is 10 structure of cavity base, and cavity base 10 is hinged upper cover 11,11 lower surface of upper cover and 10 upper surface of cavity base above
Surrounding contact site is provided with the flexible seal device 12 to complement one another, and 10 side of cavity base is provided with motor 13, motor 13
Upper cover 11 is connected to drive the folding of upper cover by transmission mechanism 14.
Embodiment 2
On the basis of embodiment 1, the present invention is in order to enable the cavity base makes its holding for storing volatile liquid
Long-time density is constant, avoids the complexity, costliness, low reliability of external infusion pumping system, is arranged inside the cavity base
After having reservoir, introducing cavity base to do reservoir, required pipeline and pump, avoid when without using adding volatile reagent
The complexity of equipment is high with cost.When cavity base stores volatile reagent, preparation flow can be carried out continuously, in not needing
Way stops preparation flow to add volatile reagent.
Embodiment 3
On the basis of embodiment 1, the present invention is in order to freeze to cavity base inside so that cavity base, which preserves, lives
Property substance when can keep activity for a long time, avoid condensing, the cavity base is internally provided with refrigerator, introduces refrigerator
Afterwards, instead of open refrigeration device, influence of the condensed water to reagent is avoided.
Embodiment 4
On the basis of embodiment 1, the present invention evaporates and aerosol cross contamination, nothing in heating process in order to prevent
It need to be placed in PCR instrument and carry out, so as to avoid the rising for the occupancy and cost for using the device space that integrated form PCR instrument brings,
Heating device is both provided with inside the cavity base and upper cover, two heating devices heat reagent, realize that enzyme is anti-
High Accuracy Constant Temperature control in answering, upper cover and mating flexible seal device are sealed cavity base, the cavity base of introducing
Heating function is all had with upper cover, preparation flow can be carried out continuously, and need not be stopped preparation flow halfway and be done in device external
Enzyme reaction, the cavity base and upper cover of introducing all have heating function.
Embodiment 5
As shown in Fig. 2, on the basis of embodiment 4, the present invention stretches into order to facilitate mechanical arm and extracts or be added reagent,
PCR plate 15 is set on the heater in the cavity base 10, and motor 13 connects upper cover 12, upper cover 12 by transmission mechanism 14
After pushing PCR plate 15, flexible seal device 11 is deformed, and forms sealing so that each hole in PCR plate forms independent
Reaction tank not will produce aerosol and pollute.
Finally it should be noted that：Obviously, the above embodiment is merely an example for clearly illustrating the present invention, and simultaneously
The non-restriction to embodiment.For those of ordinary skill in the art, it can also do on the basis of the above description
Go out other various forms of variations or variation.There is no necessity and possibility to exhaust all the enbodiments.And thus drawn
The obvious changes or variations that Shen goes out are still in the protection scope of this invention.
Claims (1)
1. a kind of low cost automatic sample Preparation equipment, including closed module, which is characterized in that under the closed module
Portion is cavity base structure, and upper cover, upper cover lower surface and cavity base upper surface surrounding contact site are hinged above cavity base
Be provided with the flexible seal device to complement one another, be provided with motor outside cavity base, motor by transmission mechanism connect upper cover from
And drive the folding of upper cover；
The cavity base is internally provided with reservoir, and reservoir is for storing volatile liquid；
Alternatively, the cavity base is internally provided with refrigerator, refrigerator freezes to cavity base inside；
Alternatively, be both provided with heating device inside the cavity base and upper cover, heating device to cavity base and upper cover into
Row heating；
The full-automatic inexpensive sample preparation device uses integrative collocation, according to different sample preparation demands in three maintenance and operations
In the liquor removing workstation that dynamic mechanical arm and accurate pipetting head are constituted, closed liquid storage module, airtight heating module, closed is organically combined
The function systems such as refrigeration module, oscillation module, Magneto separate module, mistake proofing module, flap module complete the plasmid to DNA sample
The processing such as extraction, library create, enzymatic reagent is reacted, to complete the sample preparation of whole process DNA detections on a machine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710255703.2A CN106872238B (en) | 2017-04-19 | 2017-04-19 | A kind of low cost automatic sample Preparation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710255703.2A CN106872238B (en) | 2017-04-19 | 2017-04-19 | A kind of low cost automatic sample Preparation equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106872238A CN106872238A (en) | 2017-06-20 |
| CN106872238B true CN106872238B (en) | 2018-07-27 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710255703.2A Active CN106872238B (en) | 2017-04-19 | 2017-04-19 | A kind of low cost automatic sample Preparation equipment |
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Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6576459B2 (en) * | 2001-03-23 | 2003-06-10 | The Regents Of The University Of California | Sample preparation and detection device for infectious agents |
| CN203283441U (en) * | 2013-04-26 | 2013-11-13 | 北京机械工业自动化研究所 | Device capable of automatically opening and closing box cover |
| CN104513796A (en) * | 2013-09-30 | 2015-04-15 | 王金鹤 | Novel detection device for thermal transmission in DNA amplification |
| CN203904329U (en) * | 2014-04-11 | 2014-10-29 | 珠海美路得企业发展有限公司 | Heating plate device for medicine detection equipment |
| CN106018352B (en) * | 2016-07-05 | 2017-08-22 | 南京天纵易康生物科技股份有限公司 | Photo-electric Biochemical Analyzer and its analysis method |
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| CN106872238A (en) | 2017-06-20 |
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