CN106857250A - A kind of method for promoting bletilla striata seeds to sprout - Google Patents
A kind of method for promoting bletilla striata seeds to sprout Download PDFInfo
- Publication number
- CN106857250A CN106857250A CN201710053301.4A CN201710053301A CN106857250A CN 106857250 A CN106857250 A CN 106857250A CN 201710053301 A CN201710053301 A CN 201710053301A CN 106857250 A CN106857250 A CN 106857250A
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- CN
- China
- Prior art keywords
- bletilla striata
- bletilla
- seed
- culture
- sprouted
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
A kind of method for promoting bletilla striata seeds to sprout, is related to orchid plant culture field, comprises the following steps：Configuration culture medium, and 20min that it sterilizes under 121 DEG C, 0.1Kpa states, the still uncracked capsule of collection when 15 weeks after the pollination of bletilla plant blossom, longitudinally cut capsule after cleaning and sterilizing open, by powdered seed gently shake off to media surface temperature be (25 ± 1) DEG C, 2000~2500lx of intensity of illumination, proceeds by culture under the conditions of light application time 16hd；The inventive method is easy to use, can promote the quickening of bletilla striata seeds speed of germinating, reduction incubation time for the bletilla striata, is easy to fasciation to separate, improves squamous subculture efficiency and seed germination rate.
Description
Technical field：
The present invention relates to orchid plant culture field, and in particular to a kind of method that promotion bletilla striata seeds are sprouted.
Background technology：
Bletilla is the herbaceos perennial of orchid family bletilla category, its large flower and brilliant color, and have a plump meat can hyoscine
Block pseudobulb, has ornamental value and medical value mouthful higher.Bletilla is conventional based on division propagation, but its reproductive efficiency compared with
It is low, it is difficult to the need for adapting to large-scale planting.For orchid including including bletilla, trained with tissue by aseptic seeding
Support, can in a short time obtain substantial amounts of seedling, a kind of fast and effectively propagation method of can yet be regarded as.
And current cultural method is all the traditional cultural method of comparing, it is easy to produce that incubation time is long, seed fasciation
It is difficult to separate, squamous subculture is bothersome, the low phenomenon for being unfavorable for that seed is sprouted of germination rate occurs.
The content of the invention：
The technical problems to be solved by the invention are that a kind of method of offer is easy to use, can promote the bletilla striata for the bletilla striata
Seed speed of germinating is accelerated, reduces incubation time, is easy to fasciation to separate, improves the bletilla striata of squamous subculture efficiency and seed germination rate
The method that seed is sprouted.
The technical problems to be solved by the invention are realized using following technical scheme：
A kind of method for promoting bletilla striata seeds to sprout, comprises the following steps：
(1) with 1/2MS culture mediums as minimal medium, addition 30g/L sucrose is configured to fluid nutrient medium, and by its
121 DEG C, sterilize under 0.1Kpa states 20min, after the completion of natural cooling it is standby, will configuration 1/2MS culture mediums institute in laboratory
The chemical composition for needing is configured to mother liquor, and wherein a great number of elements, trace element, inositol, vitamin is retained separately with stock solutions, iron
Salt is now with the current；
Configuration culture medium when, laboratory weigh 100L amount 1/2MS culture mediums needed for mother liquor, first plus part pure water with
With in container that sucrose dissolving is complete, then required mother liquor is added thereto to, is dissolved to 100L, adjusted with the NaOH solution of 5mol/L
Section pH value, PH is adjusted to 5.8-6.2, filling using 600mL blake bottles after completion, every bottle of filling 225mL, afterwards by height
Pressure steam sterilizer sterilizes 20min under 121 DEG C, 0.1Kpa states, after the completion of naturally cool to room temperature.
(2) the still uncracked capsule of collection when 15 weeks after the pollination of bletilla plant blossom, after it is cleaned with flowing water, then uses
75% alcohol surface sterilization 1min, then sterilize 10min in 0.1% solution, aseptic water washing 4~6 times, uses aseptic water suction
Paper is exhausted and shows residual liquid, aseptically, longitudinally cuts capsule open, capsule shell is gripped with tweezers, by powdered kind
Son is gently shaken off to media surface to proceed by culture；
(3) cultivation temperature is (25 ± 1) DEG C, 2000~2500lx of intensity of illumination, light application time 16hd, when bletilla seed
When germination and growth was to 2 months, root induction in root media, (root media are transferred to：MS+KT+6-BA)；
The beneficial effects of the invention are as follows：Method is easy to use, can promote for the bletilla striata bletilla striata seeds speed of germinating accelerate,
Incubation time is reduced, is easy to fasciation to separate, improved squamous subculture efficiency and seed germination rate.
Specific embodiment：
In order that technological means, creation characteristic, reached purpose and effect that the present invention is realized are easy to understand, tie below
Specific example is closed, the present invention is expanded on further.
Embodiment 1:
A kind of method for promoting bletilla striata seeds to sprout, comprises the following steps：
The still uncracked capsule of collection when 15 weeks after the pollination of bletilla plant blossom, after it is cleaned with flowing water, then uses
75% alcohol surface sterilization 1min, then sterilize 10min in 0.1% solution, aseptic water washing 4~6 times, uses aseptic water suction
Paper is exhausted and shows that residual liquid is standby, and the chemical composition needed for configuring 1/2MS culture mediums is configured into mother liquor in laboratory, its
Middle a great number of elements, trace element, inositol, vitamin are retained separately with stock solutions, and molysite is now with the current；
Configuration culture medium when, laboratory weigh 100L amount 1/2MS culture mediums needed for mother liquor, first plus part pure water with
With in container that sucrose dissolving is complete, then required mother liquor is added thereto to, is dissolved to 100L, adjusted with the NaOH solution of 5mol/L
Section pH value, PH is adjusted to 5.8-6.2, filling using 600mL blake bottles after completion, every bottle of filling 225mL, afterwards by height
Pressure steam sterilizer sterilizes 20min under 121 DEG C, 0.1Kpa states, after the completion of naturally cool to room temperature；
Under aseptic condition, aseptic capsule longitudinally being cut open, capsule shell being gripped with tweezers, powdered seed is gently shaken off
It is (25 ± 1) DEG C, 2000~2500lx of intensity of illumination, the culture of light application time 16hd in cultivation temperature to media surface
Under the conditions of cultivate 2 months, be transferred to root induction (root media in root media：MS+KT+6-BA)；
General principle of the invention and principal character and advantages of the present invention has been shown and described above.The technology of the industry
Personnel it should be appreciated that the present invention is not limited to the above embodiments, simply explanation described in above-described embodiment and specification this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appending claims and its
Equivalent thereof.
Claims (2)
1. a kind of method that promotion bletilla striata seeds are sprouted, it is characterised in that：Comprise the following steps：
(1) with 1/2MS culture mediums as minimal medium, addition 30g/L sucrose is configured to fluid nutrient medium, and by its 121 DEG C,
Sterilize 20min under 0.1Kpa states, after the completion of natural cooling it is standby, wherein a great number of elements, trace element, inositol, vitamin with
Stock solutions are retained separately, and molysite is now with the current；
(2) the still uncracked capsule of collection when 15 weeks after the pollination of bletilla plant blossom, by its with flowing water it is clean after, then with 75%
Alcohol surface sterilization 1min, then sterilize 10min in 0.1% solution, aseptic water washing 4~6 times, is inhaled with aseptic blotting paper
Show residual liquid to the greatest extent, aseptically, longitudinally cut capsule open, capsule shell is gripped with tweezers, powdered seed is light
Gently shake off to proceed by culture to media surface；
(3) temperature is (25 ± 1) DEG C, 2000~2500lx of intensity of illumination, light application time 16hd, when bletilla seed is sprouted when cultivating
When hair grows to 2 months, then root induction in root media is transferred to, so far promotes the process that seed is sprouted to terminate.
2. a kind of method that promotion bletilla striata seeds are sprouted, it is characterised in that：The compound method of the minimal medium is：Weigh
100L measures 1/2MS culture medium mother liquors, plus pure water then is added thereto to required mother with sucrose dissolving is complete in container
Liquid, is dissolved to 100L, and pH value is adjusted with the NaOH solution of 5mol/L, and PH is adjusted to 5.8-6.2, and 600mL is used after completion
Blake bottle is filling, every bottle of filling 225mL, is sterilized under 121 DEG C, 0.1Kpa states 20min by autoclave sterilizer afterwards,
After the completion of naturally cool to room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710053301.4A CN106857250A (en) | 2017-01-22 | 2017-01-22 | A kind of method for promoting bletilla striata seeds to sprout |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710053301.4A CN106857250A (en) | 2017-01-22 | 2017-01-22 | A kind of method for promoting bletilla striata seeds to sprout |
Publications (1)
| Publication Number | Publication Date |
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| CN106857250A true CN106857250A (en) | 2017-06-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710053301.4A Pending CN106857250A (en) | 2017-01-22 | 2017-01-22 | A kind of method for promoting bletilla striata seeds to sprout |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104137778A (en) * | 2014-07-30 | 2014-11-12 | 中国科学院南京分院东台滩涂研究院 | Method for culturing bletilla striata protocorm by means of bletilla striata seeds according to fluid suspension culture method |
| CN105325302A (en) * | 2015-12-11 | 2016-02-17 | 电子科技大学 | Method for bletilla striata seedling production based on liquid medium |
| CN106035088A (en) * | 2016-06-17 | 2016-10-26 | 丽江海贝瑞生物科技有限公司 | Method for tissue culture fast propagation of bletilla and application |
| CN106258903A (en) * | 2016-08-29 | 2017-01-04 | 浙江金石生物科技有限公司 | A kind of method of large-scale production Pseudobulbus Bletillae (Rhizoma Bletillae) seedling |
-
2017
- 2017-01-22 CN CN201710053301.4A patent/CN106857250A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104137778A (en) * | 2014-07-30 | 2014-11-12 | 中国科学院南京分院东台滩涂研究院 | Method for culturing bletilla striata protocorm by means of bletilla striata seeds according to fluid suspension culture method |
| CN105325302A (en) * | 2015-12-11 | 2016-02-17 | 电子科技大学 | Method for bletilla striata seedling production based on liquid medium |
| CN106035088A (en) * | 2016-06-17 | 2016-10-26 | 丽江海贝瑞生物科技有限公司 | Method for tissue culture fast propagation of bletilla and application |
| CN106258903A (en) * | 2016-08-29 | 2017-01-04 | 浙江金石生物科技有限公司 | A kind of method of large-scale production Pseudobulbus Bletillae (Rhizoma Bletillae) seedling |
Non-Patent Citations (1)
| Title |
|---|
| 汤兴利等: "白及种子液体悬浮培养原球茎的研究", 《安徽农业科学》 * |
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Application publication date: 20170620 |
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