CN106855507A - A kind of evaluation method of beverage antioxygenic property - Google Patents
A kind of evaluation method of beverage antioxygenic property Download PDFInfo
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- CN106855507A CN106855507A CN201610074723.5A CN201610074723A CN106855507A CN 106855507 A CN106855507 A CN 106855507A CN 201610074723 A CN201610074723 A CN 201610074723A CN 106855507 A CN106855507 A CN 106855507A
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- 235000013361 beverage Nutrition 0.000 title claims abstract description 72
- 230000003026 anti-oxygenic effect Effects 0.000 title claims abstract description 56
- 238000011156 evaluation Methods 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 claims abstract description 25
- 230000002000 scavenging effect Effects 0.000 claims abstract description 10
- 239000000126 substance Substances 0.000 claims description 39
- 230000007760 free radical scavenging Effects 0.000 claims description 37
- OHDRQQURAXLVGJ-HLVWOLMTSA-N azane;(2e)-3-ethyl-2-[(e)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound [NH4+].[NH4+].S/1C2=CC(S([O-])(=O)=O)=CC=C2N(CC)C\1=N/N=C1/SC2=CC(S([O-])(=O)=O)=CC=C2N1CC OHDRQQURAXLVGJ-HLVWOLMTSA-N 0.000 claims description 31
- 239000000243 solution Substances 0.000 claims description 28
- 239000012085 test solution Substances 0.000 claims description 28
- 239000012895 dilution Substances 0.000 claims description 24
- 238000010790 dilution Methods 0.000 claims description 24
- 238000002835 absorbance Methods 0.000 claims description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- -1 ABTS radicals Chemical class 0.000 claims description 13
- 239000007800 oxidant agent Substances 0.000 claims description 13
- 239000007864 aqueous solution Substances 0.000 claims description 12
- 230000001590 oxidative effect Effects 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 9
- 230000002292 Radical scavenging effect Effects 0.000 claims description 8
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 7
- 229930003268 Vitamin C Natural products 0.000 claims description 7
- 235000019154 vitamin C Nutrition 0.000 claims description 7
- 239000011718 vitamin C Substances 0.000 claims description 7
- USHAGKDGDHPEEY-UHFFFAOYSA-L potassium persulfate Chemical compound [K+].[K+].[O-]S(=O)(=O)OOS([O-])(=O)=O USHAGKDGDHPEEY-UHFFFAOYSA-L 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 5
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical group OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 235000019394 potassium persulphate Nutrition 0.000 claims description 4
- CHQMHPLRPQMAMX-UHFFFAOYSA-L sodium persulfate Chemical compound [Na+].[Na+].[O-]S(=O)(=O)OOS([O-])(=O)=O CHQMHPLRPQMAMX-UHFFFAOYSA-L 0.000 claims description 4
- 238000010521 absorption reaction Methods 0.000 claims description 3
- 150000002978 peroxides Chemical group 0.000 claims description 3
- JRKICGRDRMAZLK-UHFFFAOYSA-L peroxydisulfate Chemical compound [O-]S(=O)(=O)OOS([O-])(=O)=O JRKICGRDRMAZLK-UHFFFAOYSA-L 0.000 claims description 3
- 230000009467 reduction Effects 0.000 claims description 3
- 239000012452 mother liquor Substances 0.000 claims description 2
- 150000003254 radicals Chemical class 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 6
- 238000007689 inspection Methods 0.000 abstract description 5
- 230000007246 mechanism Effects 0.000 abstract description 5
- 239000003960 organic solvent Substances 0.000 abstract description 5
- 238000012827 research and development Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 239000000463 material Substances 0.000 description 8
- 230000000694 effects Effects 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000032683 aging Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000012925 reference material Substances 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229960005070 ascorbic acid Drugs 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009614 chemical analysis method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 230000036542 oxidative stress Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000005502 peroxidation Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 150000005839 radical cations Chemical class 0.000 description 1
- 239000013558 reference substance Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000000870 ultraviolet spectroscopy Methods 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/33—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using ultraviolet light
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- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention provides a kind of evaluation method of beverage antioxygenic property, it is characterised in that：Using ABTS radicals scavenging methods, the equivalent concentration of the antioxygenic property of beverage is obtained.The method that the present invention is provided, instrument and equipment requirement is low, and reagent is cheap and easy to get, organic solvent is not used, operation is simple, it is as a result reproducible, evaluation conclusion is simple and clear, is a kind of method of the evaluation beverage antioxygenic property that can be used as food inspection mechanism and food research and development, manufacturing enterprise.
Description
Technical field
The invention belongs to chemical analysis method field, in particular it relates to a kind of analysis method, drinks for evaluating
Material antioxygenic property.
Background technology
The aging of body is relevant with internal oxidative stress.Supplement antioxidant can delay body aging process.
Anti-oxidation health beverage is a kind of health food emerging in recent years.Therefore a kind of instrument and equipment requirement of exploitation is needed badly
Low, reagent is cheap and easy to get, does not use organic solvent, and operation is simple, as a result reproducible, evaluates knot
By the method that simple and clear beverage antioxygenic property is evaluated, it supplies food research and development, manufacturing enterprise and food
Testing agency applies.
Existing antioxygenic property evaluation method involve a need to Special Equipment, expensive reagent, organic solvent,
Complicated operation, and evaluation conclusion is free radical scavenging activity X%.Some beverage antioxygenic properties are higher, if with
Stoste is evaluated, due to edge effect, beverage its free radical scavenging activity base in certain high concentration range
This is constant, if being detected after beverage is diluted, the free radical scavenging activity that beverage dilution is measured is multiplied by dilution times
Rate will be far above 100%, it is impossible to the antioxygenic property of reaction beverage directly perceived, is unfavorable for the research and development water of food enterprise
The raising of the detectability of gentle food inspection mechanism.
The content of the invention
Low the invention provides a kind of requirement of instrument and equipment, reagent is cheap and easy to get, does not use organic solvent, behaviour
Make simple and easy to apply, as a result reproducible, evaluation conclusion is simple and clear, can be used as food inspection mechanism and food
A kind of method of evaluation beverage antioxygenic property that research and development, manufacturing enterprise use.
The evaluation method of the beverage antioxygenic property that the present invention is provided, it is characterised in that：Using ABTS free radicals
Removing method, obtains the equivalent concentration of the antioxygenic property of beverage.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., above-mentioned ABTS radicals scavengings method is that in the presence of polyphenoils, ABTS radical cations are by portion
Distinguish and remove, solution absorbance reduction, in same UV absorption wave band, the solution containing polyphenoils passes through
Ultraviolet absorptivity with blank group is compared, the free radical scavenging activity of acquisition.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., above-mentioned ultraviolet absorptivity is the ultraviolet absorptivity at 734nm.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., including following operation：
Operation one, the free radical scavenging activity that primary standard substance is determined using ABTS radicals scavengings method；
Operation two, the free radical scavenging activity that various concentrations beverage is determined using ABTS radicals scavengings method；
Operation three, when beverage free radical scavenging activity enter primary standard substance free radical scavenging activity the range of linearity
When interior, the dilution ratio of beverage is recorded；
Operation four, corresponding antioxygenic property base is calculated by primary standard substance concentration-free radical scavenging activity linear equation
Quasi- material concentration, is multiplied by the dilution ratio of beverage, obtains final product the equivalent concentration of the antioxygenic property of beverage.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., said reference material be vitamin C and other be used as the material of object of reference.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., specific determination step is as follows：
Step one, primary standard substance solution is taken, the ABTS test solutions that addition is prepared, lucifuge reaction after being well mixed
10-20min, determines its ultraviolet absorptivity at 734nm, deducts antioxygenic property primary standard substance solution
Absorbance, obtains AI benchmark；
Step 2, it is blank, mensuration absorbance A with the water with primary standard substance solution even0 benchmark, calculate
Free radical scavenging activity XBenchmark%；
Step 3, primary standard substance concentration-free radical scavenging activity linear equation is set up, determine antioxygen in the range of linearity
Change the free radical scavenging activity scope of performance reference material；
Step 4, a series of drink soln for preparing dilution ratios, take the beverage with primary standard substance solution even
By the ABTS test solutions that test solution, addition are prepared, lucifuge reaction 10-20min, determines it in 734nm after being well mixed
The ultraviolet absorptivity at place, deducts absorbance of the beverage by test solution, obtains AI drinks；
Step 5, it is blank, mensuration absorbance A with the water with primary standard substance solution even0 drink, calculate certainly
By base clearance rate XDrink%；
Step 6, repeat step four and step 5 are until the free radical scavenging activity X of beverageDrink% enters step 3 institute
In the free radical scavenging activity range of linearity of the primary standard substance of foundation；
Step 7, the dilution ratio for recording current beverage；
Step 8, calculate that corresponding primary standard substance is dense by primary standard substance concentration-free radical scavenging activity linear equation
Degree, is multiplied by dilution ratio of the beverage by test solution, obtains final product the antioxygenic property equivalent concentration of the beverage；
Wherein, the dilution ratio of the drink soln selected in step 4 is measured from low to high；
Said reference substance solution is 1 with the volume ratio of ABTS test solutions:5-15.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., above-mentioned ABTS test solutions by ABTS the aqueous solution and aqueous oxidizing agent solution mixed preparing.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., above-mentioned oxidant is selected from peroxide, persulfate.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., above-mentioned oxidant is selected from hydrogen peroxide, potassium peroxydisulfate, sodium peroxydisulfate.
Further, the present invention provide beverage antioxygenic property evaluation method, also with it is such the characteristics of：
I.e., the collocation method of above-mentioned ABTS test solutions is：To being added dropwise over the ABTS aqueous solution in the aqueous solution of oxidant,
Lucifuge is reacted 6-24 hours at room temperature, obtains ABTS mother liquors, and 0-10 DEG C keeps in dark place standby, and the used time takes mother liquor,
It is diluted with water to the absorbance at 432nm and is about 0.7 or so；
Wherein, the mass concentration of the aqueous solution of above-mentioned oxidant is 0.03-0.1%；
The mass concentration of the above-mentioned ABTS aqueous solution is 0.1-1%.
Effect of the invention and effect：
The analysis method that the present invention is provided, it is used to evaluate beverage antioxygenic property, the instrument that the method is used
It is UV-Vis spectrophotometry photometer.Evaluation result expression form is dense for the equivalent of antioxygenic property primary standard substance
Degree.
The principle of the method be ABTS [2,2'- join nitrogen-two (3- ethyls-benzothiazole -6- sulfonic acid) di-ammonium salts] from
Method is removed by base：In aqueous, ABTS forms a kind of as follows under the peroxidation of oxidant
The radical cation of stabilization,
The material has absorption maximum in ultraviolet-visible wave band, and 734nm is preferably used in the present invention.Anti-oxidant
In the presence of material, ABTS radical cations are partly removed, solution absorbance reduction, by with blank
The comparing of group experimental result, obtains the elimination effect to ABTS radical cations by test solution；
Ai：By the ultraviolet absorptivity at test solution 734nm；
A0：Ultraviolet absorptivity at blank group 734nm；
Represented with free radical scavenging activity (X%).
It is considered that some beverage antioxygenic properties are higher, and if being evaluated with stoste, due to edge effect,
Beverage its free radical scavenging activity in certain high concentration range is basically unchanged, if being detected after beverage is diluted,
The free radical scavenging activity that beverage dilution is measured is multiplied by dilution ratio will be far above 100%, it is impossible to reaction drink directly perceived
The antioxygenic property of material, is unfavorable for carrying for the R ＆ D Level of food enterprise and the detectability of food inspection mechanism
It is high.So, it is evaluation result expression form from equivalent concentration, so as to avoid detection in the present invention
The inaccurate problem of result.
Additionally, be can be seen that in detection process of the invention from process of the invention, it is any without adding
Organic reagent, whole detection process, its low, low cost of pollution, detection side can be completed only with the aqueous solution
Formula is simple.
The requirement of this method instrument and equipment is low, and reagent is cheap and easy to get, does not use organic solvent, and operation is simple,
Result is reproducible, and evaluation conclusion is simple and clear, can be used as food inspection mechanism and food research and development, production enterprise
A kind of method of evaluation beverage antioxygenic property that industry is used.
Brief description of the drawings
Vitamin C concentration-ABTS free radical scavenging activity % linear equations are obtained in accompanying drawing 1, embodiment.
Specific embodiment
In the present embodiment, antioxygenic property primary standard substance selects vitamin C.
Oxidant selects potassium peroxydisulfate.
The preparation of step one, ABTS test solutions：
ABTS solution is prepared：Precision weighs ABTS 0.1920g, and 50mL is settled to water.The ABTS is water-soluble
The mass concentration of liquid can also be any concentration in the range of 0.1-1%.
Potassium persulfate solution is prepared：Precision weighs potassium peroxydisulfate 0.0331g, and 50mL is settled to water.The oxygen
It can also be any concentration in the range of 0.03-0.1% that the mass concentration of the aqueous solution of agent is.The oxidant can
Selected from any peroxide or persulfate compounds.
ABTS test solutions are prepared：Two solution respectively take 10mL and are slowly mixed together (to being added dropwise in potassium persulfate solution
ABTS, now solution be transformed into by light green color blackish green), lucifuge reaction at room temperature overnight, obtains ABTS female
Liquid.4 DEG C keep in dark place standby, and the used time takes partial mother liquid, is diluted with water to the absorbance at 432nm and is about 0.7
Left and right.
The measuring and calculating of step 2, primary standard substance free radical scavenging activity：
A series of vitamin c solution of concentration is prepared, free radical scavenging activity is determined with ABTS radicals scavengings method.
Take antioxygenic property primary standard substance solution 0.2mL, the ABTS test solution 1.8mL that addition the is prepared, (inoxidizability
Energy primary standard substance solution can also be 1 with the volume ratio of ABTS test solutions:Arbitrary proportion in the range of 5-15) it is mixed
Uniform rear lucifuge reaction 10min is closed, its ultraviolet absorptivity at 734nm is determined, antioxygenic property base is deducted
Quasi- substance solution 0.2mL adds the absorbance of 1.8mL water, obtains Ai。
With 0.2mL water as blank, mensuration absorbance A0, calculate free radical scavenging activity X%.
As shown in figure 1, antioxygenic property primary standard substance concentration-free radical scavenging activity linear equation is set up, it is determined that
The free radical scavenging activity scope of antioxygenic property primary standard substance in the range of linearity.Through actual test, vitamin C
The free radical scavenging activity range of linearity by 0-60%.
The measuring and calculating of step 3, tested beverage free radical scavenging activity：
A series of drink soln of dilution ratios is prepared, beverage is determined by test solution with ABTS radicals scavengings method
Free radical scavenging activity.The ABTS test solution 1.8mL that material is prepared by test solution 0.2mL, addition are drunk, is well mixed
Lucifuge reaction 10min, determines its ultraviolet absorptivity at 734nm afterwards, deducts beverage and is added by test solution 0.2mL
The absorbance of 1.8mL water, obtains Ai.With 0.2mL water as blank, mensuration absorbance A0 is calculated freely
Base clearance rate.By beverage stoste, dilution (dilution ratio is from low to high) sequentially determine, calculate from
By base clearance rate.If free radical scavenging activity is higher than the linear model of free radical scavenging activity of antioxygenic property primary standard substance
Enclose, determined again after appropriate dilution, until beverage is entered antioxygenic property base by the free radical scavenging activity of test solution
The free radical scavenging activity range of linearity of quasi- material.
Step 4, the centinormal 1 measuring and calculating of tested beverage：
Record dilution ratio of the current beverage by test solution.It is clear by antioxygenic property primary standard substance concentration-free radical
Except rate linear equation calculates corresponding antioxygenic property primary standard substance concentration, dilution times of the beverage by test solution is multiplied by
Rate, obtains final product the antioxygenic property equivalent concentration of the beverage.
After testing, 5 times of free radical scavenging activities of dilution of the tested beverage are 46.92%, in vitamin
The free radical scavenging activity range of linearity of C.According to vitamin C concentration-free radical scavenging activity linear equation, calculate
It is 77.65 μm of ol/L to obtain corresponding vitamin C concentration, is multiplied by 5 times of dilution ratios, obtains the antioxygen of the beverage
It is 388.25 μm of ol/L to change performance equivalent.
Claims (10)
1. a kind of evaluation method of beverage antioxygenic property, it is characterised in that：Using ABTS radicals scavenging methods,
Obtain the equivalent concentration of the antioxygenic property of beverage.
2. a kind of evaluation method of beverage antioxygenic property as claimed in claim 1, it is characterised in that：
The ABTS radicals scavengings method is that in the presence of polyphenoils, ABTS radical cations are clear by part
Remove, solution absorbance reduction, in same UV absorption wave band, the solution containing polyphenoils by with sky
The ultraviolet absorptivity of white group is compared, the free radical scavenging activity of acquisition.
3. a kind of evaluation method of beverage antioxygenic property as claimed in claim 2, it is characterised in that：
The ultraviolet absorptivity is the ultraviolet absorptivity at 734nm.
4. a kind of evaluation method of beverage antioxygenic property as claimed in claim 1, it is characterised in that including such as
Lower operation：
Operation one, the free radical scavenging activity that primary standard substance is determined using ABTS radicals scavengings method；
Operation two, the free radical scavenging activity that various concentrations beverage is determined using ABTS radicals scavengings method；
Operation three, when beverage free radical scavenging activity enter primary standard substance free radical scavenging activity the range of linearity in when,
Record the dilution ratio of beverage；
Operation four, corresponding antioxygenic property primary standard substance is calculated by primary standard substance concentration-free radical scavenging activity linear equation
Matter concentration, is multiplied by the dilution ratio of beverage, obtains final product the equivalent concentration of the antioxygenic property of beverage.
5. a kind of evaluation method of beverage antioxygenic property as claimed in claim 3, it is characterised in that：
The primary standard substance is vitamin C.
6. a kind of evaluation method of beverage antioxygenic property as claimed in claim 3, it is characterised in that specific to survey
Determine step as follows：
Step one, primary standard substance solution is taken, the ABTS test solutions that addition is prepared, lucifuge reaction 10-20min after being well mixed,
Its ultraviolet absorptivity at 734nm is determined, the absorbance of antioxygenic property primary standard substance solution is deducted, obtained
AI benchmark；
Step 2, it is blank, mensuration absorbance A with the water with primary standard substance solution even0 benchmark, calculate freely
Base clearance rate XBenchmark%；
Step 3, primary standard substance concentration-free radical scavenging activity linear equation is set up, determine inoxidizability in the range of linearity
The free radical scavenging activity scope of energy primary standard substance；
Step 4, a series of drink soln for preparing dilution ratios, take tested with the beverage of primary standard substance solution even
Liquid, the ABTS test solutions that prepare of addition, lucifuge reaction 10-20min after being well mixed determines it at 734nm
Ultraviolet absorptivity, deduct beverage by test solution absorbance, obtain AI drinks；
Step 5, it is blank, mensuration absorbance A with the water with primary standard substance solution even0 drink, calculate free radical
Clearance rate XDrink%；
Step 6, repeat step four and step 5 are until the free radical scavenging activity X of beverageDrink% is set up into step 3
Primary standard substance the free radical scavenging activity range of linearity in；
Step 7, the dilution ratio for recording current beverage；
Step 8, corresponding primary standard substance concentration is calculated by primary standard substance concentration-free radical scavenging activity linear equation, multiplied
With beverage by the dilution ratio of test solution, the antioxygenic property equivalent concentration of the beverage is obtained final product；
Wherein, the dilution ratio of the drink soln selected in step 4 is measured from low to high；
The primary standard substance solution is 1 with the volume ratio of ABTS test solutions:5-15.
7. a kind of evaluation method of beverage antioxygenic property as claimed in claim 6, it is characterised in that：
The ABTS test solutions by ABTS the aqueous solution and aqueous oxidizing agent solution mixed preparing.
8. a kind of evaluation method of beverage antioxygenic property as claimed in claim 7, it is characterised in that：
The oxidant is selected from peroxide, persulfate.
9. a kind of evaluation method of beverage antioxygenic property as claimed in claim 7, it is characterised in that：
The oxidant is selected from hydrogen peroxide, potassium peroxydisulfate, sodium peroxydisulfate.
10. a kind of evaluation method of beverage antioxygenic property as claimed in claim 7, it is characterised in that：
The collocation method of the ABTS test solutions is：To being added dropwise over the ABTS aqueous solution, room in the aqueous solution of oxidant
The lower lucifuge of temperature is reacted 6-24 hours, obtains ABTS mother liquors, and 0-10 DEG C keeps in dark place standby, and the used time takes mother liquor,
It is diluted with water to the absorbance at 432nm and is about 0.7 or so；
Wherein, the mass concentration of the aqueous solution of the oxidant is 0.03-0.1%；
The mass concentration of the ABTS aqueous solution is 0.1-1%.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108548886A (en) * | 2018-06-21 | 2018-09-18 | 上海德诺产品检测有限公司 | A kind of method of catechol and its derivative the amount of migration in measurement food preserving bag |
| CN109444058A (en) * | 2018-10-13 | 2019-03-08 | 安发（福建）生物科技有限公司 | A kind of antioxidation in vitro experiment screening method of fruit drink antioxidant activity product |
| CN110954379A (en) * | 2018-09-27 | 2020-04-03 | 深圳钜运生物科技有限公司 | Method for evaluating antioxidant capacity of fruits |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105241965A (en) * | 2014-07-09 | 2016-01-13 | 西南大学 | Method of on-line quickly detecting total anti-oxidizing property of sample |
-
2016
- 2016-02-02 CN CN201610074723.5A patent/CN106855507A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105241965A (en) * | 2014-07-09 | 2016-01-13 | 西南大学 | Method of on-line quickly detecting total anti-oxidizing property of sample |
Non-Patent Citations (2)
| Title |
|---|
| 李治明等: ""皇后葵果实提取物的抗氧化活性研究"", 《广州化工》 * |
| 米书梅等: ""几种常见果蔬抗氧化活性与多酚和维生素C的关系"", 《食品工业科技》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108548886A (en) * | 2018-06-21 | 2018-09-18 | 上海德诺产品检测有限公司 | A kind of method of catechol and its derivative the amount of migration in measurement food preserving bag |
| CN110954379A (en) * | 2018-09-27 | 2020-04-03 | 深圳钜运生物科技有限公司 | Method for evaluating antioxidant capacity of fruits |
| CN109444058A (en) * | 2018-10-13 | 2019-03-08 | 安发（福建）生物科技有限公司 | A kind of antioxidation in vitro experiment screening method of fruit drink antioxidant activity product |
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