CN106853382B - Reaction vessel for optical analysis and detection - Google Patents
Reaction vessel for optical analysis and detection Download PDFInfo
- Publication number
- CN106853382B CN106853382B CN201710096158.7A CN201710096158A CN106853382B CN 106853382 B CN106853382 B CN 106853382B CN 201710096158 A CN201710096158 A CN 201710096158A CN 106853382 B CN106853382 B CN 106853382B
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- Prior art keywords
- light
- reaction vessel
- vessel
- optical analysis
- transmitting
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
- G01N31/16—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using titration
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0325—Cells for testing reactions, e.g. containing reagents
Abstract
The invention provides a reaction vessel, which comprises a vessel body with an opening at the top, wherein at least one pair of light transmission parts which are oppositely arranged and are parallel to each other are arranged on the vessel body, so that a light path can pass through the light transmission parts and the vessel body, and a light shielding layer is arranged on the periphery of the light transmission parts. The invention also provides a titration system comprising the reaction vessel.
Description
Technical Field
The invention relates to a reaction vessel for receiving a liquid to be analyzed and detected, having a wall and a top opening, through which light can be received. In addition, the invention also relates to a titration system comprising the reaction vessel.
Background
Currently, in chemical analysis and titration assays, reaction vessels are used to contain the liquid sample to be analyzed and assayed. These containers are suitable for samples, reagents, but also for the actual detection reaction. These containers usually comprise a wall and an open top, most often in the shape of a conventional cylinder, cone, etc., for containing the various liquids to be analyzed.
In particular in titration systems, the reaction vessel serves as a reaction cell, which contains an indicator, a titrant and a titrant. Commonly used titration methods include manual titration and potentiometric titration, which respectively perform titration endpoint determination by visual observation and voltage change between electrodes, and the reaction vessel is only used as a liquid-containing vessel, and the form and structure thereof do not affect the titration result, so that the titration requirements can be satisfied by using a conventional reaction vessel.
With the continuous development of the fields of chemical analysis, titration assay, etc., a technique for analyzing a liquid sample in a reaction vessel by using optics has been proposed, and accordingly, it is necessary to modify the existing conventional reaction vessel to adapt to the optical analysis and detection technique.
Disclosure of Invention
It is an object of the present invention to provide a reaction vessel which is adaptable to use optical analysis and detection techniques, the reaction vessel being capable of receiving an optical path therethrough. It is another object of the present invention to provide a titration system comprising said reaction vessel, wherein the titration is performed optically.
According to one aspect of the present invention, there is provided a reaction vessel comprising a vessel body having an opening at a top thereof, the vessel body being provided with at least one pair of light-transmitting portions arranged in opposition to each other and parallel to each other so that a light path can pass through the light-transmitting portions and the vessel body, and a light-shielding layer provided on a periphery of the light-transmitting portions.
According to a preferred embodiment of the present invention, the distance between the light-transmitting portions is in the range of 2mm to 100 mm.
According to a preferred embodiment of the present invention, the thickness of the light-transmitting portion is in the range of 0.1mm to 10 mm.
According to a preferred embodiment of the present invention, the light-shielding layer is one of a light-shielding paint, a light-shielding tape, a light-shielding fabric and a light-shielding plate.
According to a preferred embodiment of the present invention, the reaction vessel wall is further provided with an additional light-transmitting portion to which a sensor for monitoring bubbles is attached on one side of the outer wall of the reaction vessel.
According to a preferred embodiment of the present invention, the container body is further provided with an overflow hole for overflowing the excessive liquid.
According to a preferred embodiment of the present invention, the container body has an "L" shape, and the light-transmitting portions are horizontally disposed on upper and bottom walls of the container body.
According to a preferred embodiment of the present invention, the body of the container is in the shape of "t".
According to a preferred embodiment of the present invention, the light-transmitting portion is disposed in a vertical direction at both sides of the container body.
According to another aspect of the present invention, the present invention also provides a titration system, wherein the titration system comprises the above reaction vessel, a light source, an agitation device, and a titration device.
The reaction container provided by the invention not only can realize the functions of containing reaction solution and serving as a reaction vessel, but also can be applied to optical analysis and detection technical means, and meets diversified technical requirements. In addition, the invention provides a titration system comprising the reaction vessel, and provides a titration method for carrying out titration detection by using optics.
Drawings
FIG. 1 is a front view of a reaction vessel according to a first embodiment of the present invention;
FIG. 2 is a front view of a reaction vessel according to a second embodiment of the present invention;
FIG. 3 is a front view of a reaction vessel according to a third embodiment of the present invention;
FIG. 4 is a front view of a reaction vessel according to a fourth embodiment of the present invention;
FIG. 5 is a front view of a reaction vessel according to a fifth embodiment of the present invention;
FIG. 6 is a front view of a reaction vessel according to a sixth embodiment of the present invention;
figure 7 is a titration system according to the present invention.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout.
It is to be understood that the following embodiments are illustrative only and are not limiting of the invention. The scope of the invention is defined by the claims. It should also be understood that not all of the features of the embodiments described below need be included in the practice of the present invention, and that various combinations of these features are possible.
Fig. 1 shows a reaction vessel according to a first embodiment of the present invention, which comprises a vessel body 1a provided with an opening at the top, the vessel body being provided with a pair of light-transmitting portions 2a arranged oppositely and parallel to each other so that a light path can pass through the light-transmitting portions 2a and the vessel body 1a, and a light-shielding layer 3a provided on the periphery of the light-transmitting portions. Preferably, the distance h between the light-transmitting portions is in the range of 2mm to 100mm, more preferably, the distance h between the light-transmitting portions is in the range of 5mm to 50mm, and most preferably, the distance h between the light-transmitting portions is in the range of 10mm to 20 mm.
As shown in fig. 1, the vessel body 1a is further provided with an overflow hole 4, and the overflow hole 4 is located at an upper portion of the vessel body and is used for overflowing excessive liquid when the liquid in the reaction vessel is excessive.
The shape of the light-transmitting portion may be circular, rectangular, elliptical, or the like. Preferably, the light-transmitting portion is circular and has a diameter in the range of 5mm to 20 mm. When the light source is aligned with the light transmission part, the light is transmitted to the light transmission part through an optical cable, for example, so that the light path is perpendicular to the light transmission part, is emitted from one light transmission part, passes through the container body, and is emitted from the other light transmission part, and the emitted light path is received by a photosensitive receiver or is transmitted to an optical analysis detector, such as a spectrometer.
Preferably, the thickness of the light transmission portion is in the range of 0.1mm to 10mm, and more preferably, the thickness of the light transmission portion is in the range of 1mm to 2 mm. Optionally, the light-transmitting portion may be connected to the container body by gluing, or may be fused with the container body by welding. The light-shielding layer may be one selected from a light-shielding paint, a light-shielding tape, a light-shielding fabric, and a light-shielding plate. Accordingly, the means by which the opacifying layer is attached to the container body may be coating, gluing, sleeving, etc. It will be appreciated that the above examples of light-shielding layers are not intended to limit the scope and manner of attachment of the light-shielding layers, and that one skilled in the art may select light-shielding layers of other materials so as to be light-shielding at the periphery of the light-transmitting portion. The light shielding layer is arranged, so that scattering and diffraction of light can be avoided, and the analysis and detection results are more accurate.
It is to be understood that the light shielding layer may be disposed on the entire container body except for the light transmitting portion, or may be disposed on a portion of the container body in the periphery of the light transmitting portion, so that the light path can pass through the container body without scattering and diffraction. The arrangement of the light shielding layer at a part of the container body is advantageous in that it allows an operator to observe the state change of the liquid in the reaction container with eyes while ensuring the transmission of the light path. The light-shielding layer may also be disposed on the inner wall of the container body, but it should be noted that the light-shielding layer does not react with the reaction solution.
It is also preferable that the container body and the light shielding layer are made of a heat conductive material so that an external device can heat the reaction container.
In addition, the reaction container wall can be provided with an additional light-transmitting part which is separated from the light-transmitting parts which are arranged in parallel and oppositely, and a sensor for monitoring bubbles is adhered to one side of the outer wall of the reaction container.
It will be appreciated that the light-transmissive portion and the container body may be made of different materials and the connection may be one of adhesive, welding, and mechanical fastening. The container body can also be made of the same material, so that the whole container body has good light transmission, a light shielding layer is added to a part of the container body, and at least one pair of light transmission parts which are oppositely arranged and are parallel to each other is reserved for enabling a light path to pass through the container body. The light-transmitting portion is preferably made of a material having good light-transmitting properties in the visible light range of 360nm to 800 nm. Preferably, the light-transmitting portion is made of quartz glass.
Specifically, the optical properties of the quartz glass sheet are unique, and the quartz glass sheet can transmit far ultraviolet rays, is the best material for transmitting all ultraviolet rays, and can transmit visible light and near infrared spectrum. Since quartz glass is resistant to high temperature, has a very small coefficient of thermal expansion, good chemical stability, and comparable bubbles, striae, uniformity, and birefringence with common optical glass, it is an essential optical material with high-stability optical coefficient when working in various severe situations. Further, the silica glass sheet can be classified into the following three types in terms of optical properties: the far ultraviolet JGS1 is optical quartz glass fused by high-purity oxyhydrogen flame, contains a large amount of hydroxyl (2000ppm), has excellent ultraviolet transmitting performance, particularly has light transmittance of 90 percent at 185 mu m in a short-wave ultraviolet region, has a strong absorption peak at 2730nm in a synthetic quartz glass test, and is an excellent optical material in a waveband range of 185 mu m-2500 mu m; ultraviolet JGS2, which is an optical material melted by high-purity oxyhydrogen flame, has a hydroxyl content of 100ppm to 200ppm, contains tens of ppm of metal impurities, has a strong absorption peak at 2730nm, and is an excellent optical material within a wave band range of 220 μm to 2500 μm; and infrared JGS3, which is quartz glass produced by using quartz raw materials and a vacuum electric melting method, contains dozens of ppm of metal impurities, has small bubbles, a granular structure and stripes, has high infrared transmittance, has light transmittance of more than 85 percent, and is an excellent optical material within the waveband range of 260-3500 mu m. The material of the light-transmitting sheet can be selected as desired by those skilled in the art. The unit "ppm" refers to the concentration expressed in parts per million of the mass of solute in the mass of the entire solution, also referred to as parts per million concentration.
In fig. 1, a container body 1a is substantially "L" shaped, and light transmission portions 2a are horizontally arranged on an upper wall and a bottom wall of the container body, and are arranged opposite to each other and parallel to each other, so that a light path incident vertically passes through the container body 1a and is emitted.
Fig. 1 schematically shows a reaction vessel according to a first embodiment of the invention, which may take other forms, and second to sixth embodiments of the invention are described below with reference to fig. 2 to 6.
Fig. 2 shows a reaction vessel according to a second embodiment of the present invention, and as shown in fig. 2, differs from the reaction vessel of the first embodiment in that a vessel body 1b is generally conical in shape and has one protruding portion, a light-transmitting portion 2b is horizontally arranged on an upper wall and a bottom wall of the protruding portion of the vessel body 1b, two light-transmitting portions are oppositely arranged and parallel to each other, and a light-shielding layer 3b is coated on the vessel body at the periphery of the light-transmitting portion 2 b.
Fig. 3 shows a reaction vessel according to a third embodiment of the present invention, and as shown in fig. 3, differs from the reaction vessel of the first embodiment in that the vessel body 1c is substantially "j" shaped and has two projections on which light-transmitting portions may not be provided, respectively.
Fig. 4 shows a reaction vessel according to a fourth embodiment of the present invention, and as shown in fig. 4, differs from the reaction vessel of the second embodiment in that the bottom surface of the projection of the vessel body 1d and the bottom surface of the vessel body 1d are not on one plane any more.
In the above embodiment, the light-transmitting portion is arranged horizontally, which is advantageous in that the mixed state of the solution is not affected, and the parallel light-transmitting sheets at the protruding positions are free from the influence of the vortex of the liquid, enabling the transmitted light to truly reflect the state of being absorbed by the solution.
Fig. 5 and 6 show front views of reaction vessels according to fifth and sixth embodiments of the present invention, respectively. In fig. 5, a container body 1e is a concave container having a circular arc bottom, and light transmission portions are arranged along a vertical direction 2e and are oppositely arranged on both sides of a concave portion of the container body 1 e. In fig. 6, a container body 1f is an outwardly convex container having a circular arc-shaped bottom, and light-transmitting portions are arranged along a vertical direction 2f, oppositely arranged on both sides of a projection of the container body 1 f. It is to be understood that the light-transmitting portions may also be arranged oppositely and in parallel on both sides of the container body.
The fifth and sixth embodiments of the present invention show the light-transmitting portions arranged vertically, which is advantageous in that the reaction vessel is simple in structure and easy to mold.
As is apparent from the above-described embodiments, the reaction vessel of the present invention may have various shapes and forms, and the above-described embodiments do not limit the scope of the claimed reaction vessel of the present invention, and a person skilled in the art may design a specific shape of the reaction vessel according to actual circumstances such as installation space, arrangement, and the like.
Fig. 7 shows a titration system according to the invention comprising a reaction vessel 1, a light source 4, a stirring device 5 and a titration device 6. The titration system further comprises a data processing and control unit for recording data and controlling the titration apparatus.
The light source 4 includes an incident light path and a verification light path, the incident light path is reflected and then enters the reaction container, and the verification light path is reflected and then directly received for verifying the incident light path.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (7)
1. A reaction vessel for optical analysis and detection comprises a vessel body with an opening at the top, and is characterized in that the vessel body is provided with at least one pair of light-transmitting parts which are oppositely arranged and have mutually parallel planes, so that a light path can pass through the light-transmitting parts and the vessel body, and a light shielding layer is arranged on the periphery of the light-transmitting parts;
the container body side wall has at least one protrusion, and the light-transmitting portion is horizontally disposed on an upper wall and a bottom wall of the protrusion.
2. The reaction vessel for optical analysis and detection according to claim 1, wherein the distance between the light-transmitting portions is in the range of 2mm to 100 mm.
3. The reaction vessel for optical analysis and detection according to claim 2, wherein the thickness of the light-transmitting portion is in the range of 0.1mm to 10 mm.
4. The reaction vessel for optical analysis and detection as claimed in claim 1, wherein the light shielding layer is one of a light shielding paint, a light shielding tape, a light shielding fabric and a light shielding plate.
5. The reaction vessel for optical analysis and detection as claimed in claim 2, wherein the reaction vessel wall is further provided with an additional light-transmitting portion, and the light-transmitting portion is bonded with a bubble-monitoring sensor on one side of the outer wall of the reaction vessel.
6. The vessel according to claim 1, wherein the vessel body further comprises an overflow hole for overflowing the excess liquid from the reaction vessel.
7. A titration system, characterized in that it comprises a reaction vessel for optical analysis and detection according to any one of claims 1 to 6, a light source, a stirring device and a titration device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710096158.7A CN106853382B (en) | 2017-02-22 | 2017-02-22 | Reaction vessel for optical analysis and detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710096158.7A CN106853382B (en) | 2017-02-22 | 2017-02-22 | Reaction vessel for optical analysis and detection |
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| Publication Number | Publication Date |
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| CN106853382A CN106853382A (en) | 2017-06-16 |
| CN106853382B true CN106853382B (en) | 2022-06-28 |
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| CN201710096158.7A Active CN106853382B (en) | 2017-02-22 | 2017-02-22 | Reaction vessel for optical analysis and detection |
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