CN106841464A - Ellagic acid and 3,3` dimethoxy ellagic acid detection method of content in a kind of China fir parasitism - Google Patents
Ellagic acid and 3,3` dimethoxy ellagic acid detection method of content in a kind of China fir parasitism Download PDFInfo
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- CN106841464A CN106841464A CN201710066010.9A CN201710066010A CN106841464A CN 106841464 A CN106841464 A CN 106841464A CN 201710066010 A CN201710066010 A CN 201710066010A CN 106841464 A CN106841464 A CN 106841464A
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- ellagic acid
- china fir
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Abstract
The invention discloses ellagic acid and 3,3' dimethoxy ellagic acid detection method of content in a kind of China fir parasitism, detection uses the liquid chromatograph, chromatographic condition to be：Chromatographic column is Eclipse XDB C18(250mm × 4.6mm, 5 μm)；Mobile phase is the phosphate aqueous solution of methyl alcohol 0.2%；Flow velocity is 0.8 mLmin‑1；Detection wavelength is 254nm；Column temperature is 25 DEG C；Sample size is 10 μ L；The theoretical cam curve of ellagic acid is not less than 3000 with this understanding；The theoretical cam curve of 3,3' dimethoxy ellagic acids is not less than 5000.Content assaying method that the present invention is used is easy, quick, with preferable stability and reappearance, can be used for the assay of ellagic acid and 3,3' dimethoxy ellagic acid in China fir parasitism medicinal material；Content difference of the composition in different sources China fir parasitism medicinal material is obvious, for the development of resources of China fir parasitism medicinal material provides reference frame.
Description
Technical field
The present invention relates to the assay method of special component content in medicinal plant, in particularly a kind of China fir parasitism ellagic acid and
3,3'- dimethoxy ellagic acid detection method of content.
Background technology
China fir is parasitic for Loranthaceae sheath flower plant sheath is spent（Latin name：Macrosolen cochinchinensis
（Lour.）Van Tiegh.）Stem branch.Plant sheath is spent, and is distributed in the ground such as south China, southwestern and Fujian, Tibet.With wind-damp dispelling,
The effect of filling liver kidney, promoting blood circulation and stopping pain, cough-relieving, stop dysentery.Arthralgia pain due to rheumatism is cured mainly, soreness of waist and knee joint is had a dizzy spell, alopecia, traumatic injury,
Hemorrhoid gall, cough, hemoptysis, dysentery.Specifically, China fir is parasitic to have following effect：1. China fir parasitism is the complete of plant Fu Shi sheaths flower
Strain, harvests among summer and autumn, and mild-natured, slightly sweet flavor has functions that cough-relieving and the scattered stasis of blood.Usually it is used for peratodynia and hernia
The treatment of the various disease conditions such as gas and internal injury hemoptysis；2. China fir parasitism can control peratodynia：China fir parasitism plays the role of regulating qi-flowing for relieving pain, to the mankind
Peratodynia have good therapeutic action, can individually eat can also it is simultaneously fried with lean pork after it is edible, with lean pork one
When playing fried edible, therapeutic effect can be outstanding.This eating method is similarly adapted to the hernia of the mankind, and therapeutic effect
Compare good；3. China fir parasitism can control bitter wound and hemoptysis：The parasitic bitter wound to the mankind of China fir has treatment well with tripod hemoptysis symptom
Effect, in treatment based on for oral administration, can take parasitic 30 grams of China fir clear water it is fried after take, take once a day and be used in conjunction seven
My god, effect is pretty good；4. China fir parasitism can control arthralgia：Parasitic arthralgia and the rheumatism treatment effect to the mankind of China fir is obvious, is controlling
Treating both can be fried rear for oral administration, it is also possible to which liquid cleans the position of oneself pain, can also play a part of anti-inflammatory analgetic and detumescence.Through
Research, various chemical compositions, Chinese document are contained in China fir parasitism：Chinese experimental pharmacology of traditional Chinese medical formulae magazine, the 3rd phase hair of volume 21 in 2015
Table《China fir parasitic chemical composition》, author：Lu Guoshou, Lu Wenjie, Chen Jiayuan etc.；Unit：Guangxi traditional Chinese medicine research institute, author is to China fir
Parasitic chemical composition is studied, therefrom isolated and identify 10 chemical compositions, respectively lupeol（1）, ring
Eucalyptus enol（2）, epifriedelinol（3）,（23Z）- 9,19-cycloart-23-ene-3 α, 25-diol (4), suberone（5）, positive three
Dodecanol（6）, cupreol（7）, lacceroic acid（8）, rutin（9）Orientoside（10）.And the present inventor's research finds that China fir is posted
Life has certain prevention effect to tumour, through to the parasitic constantly Separation Research of China fir, the aldehydes matter that discovery wherein contains is to tumour
With activity higher, aldehydes matter ellagic acid and 3,3'- dimethoxy ellagic acid in inventor's separation and Extraction China fir parasitism.
Ellagic acid (2,3,7,8-tetrahydroxy benzopyrano5,4,3 one cdebenzopyran-5,10 1
Dione) it is a kind of natural polyphenol component for being widely present in the plant tissues such as various mushy fruits, nut, ellagic acid and tri-chlorination
In blueness, it is in yellow to meet sulfuric acid to the chromogenic reaction of iron.Ellagic acid shows to induce canceration and other various cancerations to chemical substance
There is obvious inhibitory action, particularly to have suppress well to colon cancer, the cancer of the esophagus, liver cancer, lung cancer, tongue and skin neoplasin etc. and make
With.
For the parasitic chemical composition of further clear and definite China fir, research that we have carried out chemical composition to it is simultaneously therefrom separated
Identification obtains two phenoloids, respectively ellagic acid and 3,3'- dimethoxy ellagic acid.Ellagic acid and 3,3'- dimethoxies
Base ellagic acid is to be got from China fir parasitism first, while the HPLC content assaying methods of its compound are also established first, and
Tentatively compare their changes of contents in different sources China fir parasitism.Ellagic acid not only has obvious antioxidation, also
There are certain inhibitory action, or even the apoptosis that cancer cell can be caused to various cancerations.The research that this experiment is carried out, to develop with China fir
Parasitism is the medicine of raw material, health products, natural are provided and manage certain opinion foundation, China fir is colonized in medical, health care very
Widely application is played to cosmetic field.
The content of the invention
Easy to operate it is an object of the invention to provide one kind, the degree of accuracy is high, ellagic acid and 3 in reproducible China fir parasitism,
The assay method of 3'- dimethoxy ellagic acid contents.
What the present invention was realized in：
Ellagic acid and 3,3'- dimethoxy ellagic acid detection method of content in China fir parasitism of the present invention, comprise the following steps：
（A）The preparation of reference substance storing solution：Precision weighs ellagic acid reference substance and 3,3'- dimethoxy ellagic acid reference substance, puts
In same 250mL measuring bottles, add 30-50ml methyl alcohol, ultrasonic dissolution 15-20min to fully transparent, and with methanol dilution to carving
Degree, as reference substance storing solution.
（B）The preparation of need testing solution:China fir parasitism medicinal material is taken, 40-80 mesh, accurately weighed its weight, essence are crushed in advance
The ethanol of close addition 60%（g/g）, weighed weight, flow back 40-70 min, preferably 55-65min in the bath that discharges water, and lets cool, then weighed
Weight, the weight of less loss is supplied with 60% ethanol (g/g), is shaken up, and is filtered through 0.45 μm of miillpore filter, obtains final product need testing solution.
(C) reference substance and test sample sample injection liquid chromatograph for preparing are taken, sample introduction, detection is obtained final product；Chromatostrip
Part：Chromatographic column is Eclipse XDB-C18(250mm × 4.6mm, 5 μm)；Mobile phase is the % phosphate aqueous solutions of methyl alcohol -0.2, volume
Than being 47:53；Flow velocity is 0.8 mLmin-1；Detection wavelength is 254nm；Column temperature is 25 DEG C；Sample size is 10 μ L；In this condition
The theoretical cam curve of lower ellagic acid is not less than 3000；The theoretical cam curve of 3,3'- dimethoxy ellagic acids is not less than 5000.
Require that repeatability, precision to detection method etc. are tested according to above-mentioned condition, detailed process is：
First, the preparation of reference substance storing solution
Precision weighs the mg of ellagic acid reference substance 15.31, the mg of 3,3'- dimethoxy ellagic acid reference substance 9.43, puts same 250
In mL measuring bottles, plus appropriate methyl alcohol, ultrasonic dissolution and with methanol dilution to scale, as reference substance storing solution.The concentration of ellagic acid
It is 61.24 μ gmL-1, the concentration of 3,3'- dimethoxy ellagic acids is 37.72 μ gmL-1。
2nd, the preparation of need testing solution
Learn from else's experience the g of medicinal material about 0.5 of crushing, accurately weighed its weight, and precision adds 60 % ethanol, and weighed weight discharges water bath last time
60 min are flowed, is let cool, then weighed weight, the weight of less loss is supplied with 60 % ethanol, shake up, filtered through 0.45 μm of miillpore filter,
Obtain final product need testing solution.
3rd, the investigation of linear relationship
Precision pipettes 0.5,1,3,5,7,9 mL from reference substance storing solution respectively, puts respectively in 10 mL volumetric flasks, adds methyl alcohol
Scale is settled to, is shaken up, filtered, obtain final product the mixed reference substance solution of series concentration.The control of above-mentioned 6 various concentrations is taken respectively
The μ L of product solution 10 are determined by chromatographic condition sample introduction under claim, and as abscissa, peak area is vertical to the concentration with reference substance solution
Coordinate, draws standard curve.Result draws the linear equation of ellagic acid：Y = 27856X + 3884.5,r= 0.9998；3,
The linear equation of 3'- dimethoxy ellagic acids：Y = 39151X - 4588.1,r = 0.9998.Result shows that ellagic acid is compareed
It is in good linear relation, 3,3'- dimethoxy ellagic acids in 0.0306 μ g~0.5512 μ g ranges that product solution is in sample size
It is in good linear relation in 0.0189 μ g~0.3395 μ g ranges that reference substance solution is in sample size.
4th, Precision Experiment
It is 18.372 μ gmL that precision draws ellagic acid concentration-1It is 11.316 μ g with 3,3'- dimethoxy ellagic acids concentration
mL-1The μ L of reference substance solution 10, in injection high performance liquid chromatograph, by claim chromatographic condition, continuous sample introduction 6 times is determined
Peak area.As a result ellagic acid peak area RSD values are 0.17 %, and 3,3'- dimethylellagic acid peak area RSD values are 0.26 %, table
Bright high performance liquid chromatograph precision used is very good.
5th, stability test
Take China fir parasitism medicinal material（The place of production：Woods on Guangxi）Meal about 0.5 g, it is accurately weighed, prepared by test sample preparation method, room temperature
Preserve, respectively 0,2,4,8,12,24 h are accurate to draw the μ L of need testing solution 10, injects high performance liquid chromatograph, will by right
Ask regulation chromatographic condition sample introduction to determine, investigate its stability.In results sample the RSD values of ellagic acid peak area be 0.46 %, 3,
The RSD values of 3'- dimethoxy ellagic acid peak areas are 1.01%.Result shows that need testing solution keeps stabilization in 24 h.
6th, replica test
Take China fir parasitism medicinal material（The place of production：Woods on Guangxi）The g of meal about 0.5, accurately weighed weight takes 6 parts, on request test sample altogether
It is prepared by the preparation method of solution.Precision draws the μ L of need testing solution 10, is determined by the lower chromatographic condition sample introduction of claim regulation,
It is 0.738 mgg to calculate ellagic acid content-1, its RSD value is 1.64 %；The content of 3,3'- dimethoxy ellagic acids is 0.443
mg·g-1, its RSD value is 2.45 %.
7th, it is loaded recovery test
Precision is drawn in the mL round-bottomed flasks of mL to 100 of reference substance storing solution 3, volatilizes solvent in water-bath, 6 parts of parallel preparation, then
The China fir parasitism medicinal material of known content is added in every portion（The place of production：Woods on Guangxi）The g of meal about 0.25, test sample is molten on request
It is prepared by the preparation method of liquid.Precision draws the μ L of need testing solution 10, and chromatographic condition determines tan flower in each sample respectively on request
Acid, the content of 3,3'- dimethoxy ellagic acids, calculate the rate of recovery（Ellagic acid content is with 0.738 mgg in China fir parasitism medicinal material-1
Meter, the content of 3,3'- dimethoxy ellagic acids is with 0.443 mgg-1Meter）, the results are shown in Table 1.
The beneficial effects of the invention are as follows：
The detection method of ellagic acid and 3,3'- dimethoxy ellagic acid content, molten to reference substance respectively in the parasitism to China fir of the invention
Liquid and need testing solution are measured the chromatography of ellagic acid and 3,3'- dimethoxy ellagic acids under chromatographic condition of the present invention
Figure, and by the linear relationship to ellagic acid and the peak area response of 3,3'- dimethoxy ellagic acid, precision, stability,
The measure of the aspect such as repeatability and average recovery, so as to grasp ellagic acid and 3,3'- dimethoxy ellagic acid in China fir parasitism contain
The assay method of amount.This assay method is simple to operate, the degree of accuracy is high, reproducible, is worth in China fir parasitism ellagic acid and 3,3'- bis-
The aspect popularization and application of methoxyl group ellagic acid assay.
Brief description of the drawings：
Fig. 1：3,3'- dimethoxy ellagic acid structural formulas；
Fig. 2：Ellagic acid structural formula；
Fig. 3：Ellagic acid and 3,3'- dimethoxy tans in ellagic acid and 3,3'- dimethoxy ellagic acid standard specimens and China fir parasitism medicinal material
Spend sour liquid chromatogram.
Specific embodiment
Embodiment one
In Guangxi Gold show, Nanning and on Guangxi, woods takes China fir parasitism medicinal material to inventor respectively, and to the ellagic acid in China fir parasitism
Assay is carried out with 3,3'- dimethoxys ellagic acid, step is as follows：
（A）The preparation of reference substance storing solution
Precision weighs the mg of ellagic acid reference substance 15.31, the mg of 3,3'- dimethoxy ellagic acid reference substance 9.43, puts same 250
In mL measuring bottles, plus methyl alcohol 30-50ml, ultrasonic dissolution 15-20min, and with methanol dilution to scale, as reference substance storing solution.
The concentration of ellagic acid is 61.24 μ gmL-1, the concentration of 3,3'- dimethoxy ellagic acids is 37.72 μ gmL-1。
（B）The preparation of need testing solution
Learn from else's experience the g of medicinal material about 0.5 of crushing, accurately weighed its weight, and precision adds 60 % ethanol（g/g）, weighed weight discharges water
Flow back 60 min in bath, lets cool, then weighed weight, with 60 % ethanol（g/g）, the weight of less loss is supplied, shake up, through 0.45 μm
Miillpore filter is filtered, and obtains final product need testing solution.
(C) reference substance and test sample sample injection liquid chromatograph for preparing are taken, sample introduction, detection is obtained final product；Chromatostrip
Part：Chromatographic column is Eclipse XDB-C18(250mm × 4.6mm, 5 μm)；Mobile phase is the % phosphate aqueous solutions of methyl alcohol -0.2, volume
Than being 47:53；Flow velocity is 0.8 mLmin-1；Detection wavelength is 254nm；Column temperature is 25 DEG C；Sample size is 10 μ L；In this condition
The theoretical cam curve of lower ellagic acid is not less than 3000；The theoretical cam curve of 3,3'- dimethoxy ellagic acids is not less than 5000.
Testing result is shown in Table 3.
Test result indicate that, the HPLC methods that this experiment is used determine ellagic acid and 3,3'- dimethoxy tan flower in China fir parasitism medicinal material
The content of acid, method is simple and convenient, strong operability, is China fir parasitism quality of medicinal material controlling party with preferable stability reappearance
Method provides reference, is that the research of ellagic acid and 3,3'- dimethoxy ellagic acid content assaying method provides important evidence.
Claims (5)
1. ellagic acid and 3,3'- dimethoxy ellagic acid detection method of content during a kind of China fir is parasitic, it is characterised in that：Including as follows
Step：
（A）The preparation of reference substance storing solution：Precision weighs ellagic acid reference substance and 3,3'- dimethoxy ellagic acid reference substance, puts
In same 250mL measuring bottles, part methyl alcohol is added, ultrasonic dissolution and with methanol dilution to scale, as reference substance storing solution；
（B）The preparation of need testing solution:Learn from else's experience crushing China fir parasitism medicinal material, accurately weighed its weight, precision add 60% ethanol
（g/g）, weighed weight, flowed back 40-70 min in the bath that discharges water, and lets cool, then weighed weight, and less loss is supplied with 60% ethanol (g/g)
Weight, shakes up, and is filtered through 0.45 μm of miillpore filter, obtains final product need testing solution；
(C) reference substance and test sample sample injection liquid chromatograph for preparing are taken, sample introduction, detection is obtained final product；Chromatographic condition：Color
Spectrum post is Eclipse XDB-C18(250mm × 4.6mm, 5 μm)；Mobile phase is the % phosphate aqueous solutions of methyl alcohol -0.2；Flow velocity is 0.8
mL·min-1；Detection wavelength is 254nm；Column temperature is 25 DEG C；Sample size is 10 μ L；The theoretical cam curve of ellagic acid with this understanding
It is not less than 3000；The theoretical cam curve of 3,3'- dimethoxy ellagic acids is not less than 5000.
2. ellagic acid and 3,3'- dimethoxy ellagic acid detection method of content, its feature during China fir is parasitic according to claim 1
It is：The reference substance storing solution is prepared and adds 30-50ml methyl alcohol, ultrasonic dissolution 15-20min.
3. ellagic acid and 3,3'- dimethoxy ellagic acid detection method of content, its feature during China fir is parasitic according to claim 1
It is：The China fir parasitism medicinal material is needed in advance through coarse crushing, is crushed to 40-80 mesh.
4. ellagic acid and 3,3'- dimethoxy ellagic acid detection method of content, its feature during China fir is parasitic according to claim 1
It is：The preferred 55-65min of water-bath return time in the need testing solution preparation.
5. ellagic acid and 3,3'- dimethoxy ellagic acid detection method of content, its feature during China fir is parasitic according to claim 1
It is：The phosphate aqueous solution of mobile phase methanol -0.2% volume ratio is 47:53.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114609264A (en) * | 2021-12-17 | 2022-06-10 | 新疆维吾尔药业有限责任公司 | Method for determining content of ellagic acid in rose massecuite |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101788537A (en) * | 2009-09-28 | 2010-07-28 | 贵州益康制药有限公司 | Method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials |
| CN104730166A (en) * | 2015-03-25 | 2015-06-24 | 贵阳医学院 | Quantitative determination method for 3,3'-dimethyl ellagic acid and 3,3',4'-trimethyl ellagic acid in polygonum runcinatum |
-
2017
- 2017-02-06 CN CN201710066010.9A patent/CN106841464A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101788537A (en) * | 2009-09-28 | 2010-07-28 | 贵州益康制药有限公司 | Method for measuring content of ellagic acid ingredients in euscaphis japonica medicinal materials |
| CN104730166A (en) * | 2015-03-25 | 2015-06-24 | 贵阳医学院 | Quantitative determination method for 3,3'-dimethyl ellagic acid and 3,3',4'-trimethyl ellagic acid in polygonum runcinatum |
Non-Patent Citations (3)
| Title |
|---|
| 莫凯荃 等: "杉寄生中酚性化合物的提取分离及含量测定", 《中国实验方剂学杂志》 * |
| 陆国寿 等: "杉寄生化学成分", 《中国实验方剂学杂志》 * |
| 陆国寿 等: "杉寄生的脂溶性成分分析", 《广西科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114609264A (en) * | 2021-12-17 | 2022-06-10 | 新疆维吾尔药业有限责任公司 | Method for determining content of ellagic acid in rose massecuite |
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