CN106834361A - The method that stalk produces cellulosic ethanol - Google Patents

The method that stalk produces cellulosic ethanol Download PDF

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Publication number
CN106834361A
CN106834361A CN201710181164.2A CN201710181164A CN106834361A CN 106834361 A CN106834361 A CN 106834361A CN 201710181164 A CN201710181164 A CN 201710181164A CN 106834361 A CN106834361 A CN 106834361A
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fermentation
stalk
cellulosic ethanol
yeast
treatment
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王建业
刘玉金
葛健
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Heilongjiang Jianye In Bio Energy Co Ltd
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Heilongjiang Jianye In Bio Energy Co Ltd
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12FRECOVERY OF BY-PRODUCTS OF FERMENTED SOLUTIONS; DENATURED ALCOHOL; PREPARATION THEREOF
    • C12F3/00Recovery of by-products
    • C12F3/02Recovery of by-products of carbon dioxide
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14Fungi; Culture media therefor
    • C12N1/16Yeasts; Culture media therefor
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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Abstract

A kind of method that stalk produces cellulosic ethanol.Existing cellulosic ethanol production technique is mainly the agricultural wastes such as stalk and is crushed, raw material is pre-processed into pretreatment unit after crushing, material is digested into enzymolysis device after pretreatment, enzymolysis mixed liquor carries out separation of solid and liquid into separator, enzymolysis liquid glucose is proportionally added into zymophyte and is fermented into fermentation tank, and zymotic fluid obtains cellulosic ethanol through distilling apparatus separating-purifying.A kind of method that stalk produces cellulosic ethanol, comprises the following steps:(1)The treatment of stalk early stage and thermal and hydric environment pretreatment;(2)Batch mixing and enzymatic saccharification are processed;(3)Fermentation purification processes;(4)Ferment the CO for producing2Treatment;(5)The useless wine with dregs treatment of distillation;(6)Yeast fermentation after fermentation mash separating thallus albumen.The present application relates to biorefinery field, stalk production cellulosic ethanol and five kinds of methods of byproduct are more particularly to applied to.

Description

The method that stalk produces cellulosic ethanol
Technical field:
The present invention relates to biorefinery field, more particularly to a kind of stalk production cellulosic ethanol and five kinds of methods of byproduct.
Background technology:
The exploitation of new energy is to solve China's energy wilderness demand main method for lacking present situation relative with the energy, is also to solve ring The only way which must be passed of border problem, new energy has the advantages that high efficiency, low stain and sustainability, mainly including nuclear energy, wind energy, water Energy, solar energy and biomass energy etc., wherein biomass energy are even more a kind of unique reproducible carbon source, can change into conventional consolidating State, liquid or gaseous fuel;By agricultural wastes carry out recycling treatment production biomass energy, can not only become give up into Treasured, can reduce pollutant emission, environmental protection again, due to the structural complexity and the efficient fiber of shortage of natural cellulosic feedstocks Plain enzyme preparation so that current cellulosic ethanol production enterprise's production capacity is higher, single production cellulosic ethanol product is difficult to reality Now profit turns into the bottleneck of industry development;Existing cellulosic ethanol production technique is mainly the agricultural wastes such as stalk carries out powder Broken, raw material is pre-processed into pretreatment unit after crushing, and material is digested into enzymolysis device after pretreatment, and enzymolysis is mixed Close liquid carries out separation of solid and liquid into separator, and enzymolysis liquid glucose is proportionally added into zymophyte and is fermented, ferments into fermentation tank Liquid obtains cellulosic ethanol through distilling apparatus separating-purifying, and the solid slag of separation is burnt as fuel;And prior art The product variety of scheme production is single, and production cost is higher;Raw materials for production need to carry out pulverization process;Enzymolysis liquid need to carry out solid-liquid point From so that Partial digestion liquid glucose loses, and also increases technological process of production complexity;Enzymolysis slag charge carries out burning reduction product and adds Value, is not implemented making full use of for raw material full constituent.
The content of the invention:
Cellulosic ethanol is produced using stalk it is an object of the invention to provide one kind, and obtains five kinds of methods of byproduct simultaneously.
Above-mentioned purpose is realized by following technical scheme:
The method that stalk produces cellulosic ethanol, comprises the following steps:(1)The treatment of stalk early stage and thermal and hydric environment pretreatment;(2) Batch mixing and enzymatic saccharification are processed;(3)Fermentation purification processes;(4)Ferment the CO for producing2Treatment;(5)The useless wine with dregs treatment of distillation;(6)Ferment Mother's fermentation after fermentation mash separating thallus albumen, described fermentation purification processes process is:After through enzymolysis, liquid glucose is cooled to 32 20% yeast is accessed after DEG C carries out alcoholic fermentation, and fermentation time 70h carries out temperature control, hair is taken away using frozen water in fermentation process The fermentation fever that ferment is produced, control fermentation temperature is in 30-35 DEG C, residual sugar<0.3% is considered as fermentation ends, terminates the alcohol hair of fermentation Ferment mash delivers to distilling apparatus and carries out distilation and obtain cellulosic ethanol after precipitating 48h yeast separations, and distillation is using double Thick column differential-pressure distillation device carries out distillation procedure, and operation temperature is 103-108 DEG C of thick tower, 0.06-0.15mpa of tower top pressure, 85-95 DEG C of combined column temperature, tower top pressure-0.05-- 0.08mpa;Smart 120-128 DEG C of tower temperature degree, tower top pressure 0.06- 0.15mpa。
The method that described stalk produces cellulosic ethanol, described stalk early stage treatment and thermal and hydric environment preprocessing process For:Enter alcohol production device after maize straw is collected into bundling, stacking storage, conveying crushing, dedusting removal of impurities, drying, briquetting Area, the briquetting stalk of 15% moisture delivers to hot water environment after Water spray pre-soaking, washing after moisture reaches 60% in device area In formula plug flow reactor, 1.3-1.8mpa steam is passed through in the reactor, ton stalk needs 0.2-0.5t of steam, stop 12-15min is reacted.
The method that described stalk produces cellulosic ethanol, described batch mixing and enzymatic saccharification processing procedure be:Through hydro-thermal Stalk material delivers to enzymatic vessel by blender after environmental treatment, added in blender liquid NaOH regulation PH to 4.8- 5.0,2-5kg/ tons of stalk of liquid NaOH additions, cellulase 10kg/t stalks carry out enzymatic saccharification, controlled enzymatic hydrolysis temperature 50- 55℃。
A kind of carbon dioxide of the method by-product of described stalk production cellulosic ethanol, the CO that described fermentation is produced2 Processing procedure is:Ferment the CO for producing2Dissolved organic matter gas is removed by Water spray washing, the CO2 gases warp after purification 0.06% liquor potassic permanganate carries out spray washing and removes insoluble grease, and first point of water is crossed by the gas after two-step purification Boosted into carbon-dioxide gas compressor after tank point water, after boosting to 3.0MPa, proceeding through the first cooler, second point of water Tank and drying bed are further dehydrated, and the CO2 after dehydration enters adsorbent bed after the second cooler is cooled down, and carries out at removing impurities matter Reason, adsorbent bed is by eight kinds of different types of adsorbents layered arrangement in proportion, it is ensured that efficient impurity absorption curve, is purifying One of key equipment, the forecooler and pressure of -30 DEG C of CO2 entrance after purification are liquefied for the liquefier of 3.0MPa, cold Source is liquefied ammonia;Liquefier liquid CO 2 out enters in finished pot.
A kind of mycoprotein of the method by-product of described stalk production cellulosic ethanol, described yeast fermentation after fermentation Mash processing procedure is:Stop fermentation stirring fermentation liquid after yeast fermentation and staticly settle 48h, the sample examination in the middle part of tank body It is considered as sedimentation when distiller's yeast cell number is less than 2,000,000 plants/ml in karusen flow container to terminate, centrifuge obtains yeast thalline egg after separating White feed, through being dried to obtain commodity mycoprotein.
Beneficial effects of the present invention:
1. stalk of the invention produces the agricultural wastes such as cellulosic ethanol and five kinds of methods of byproduct, stalk in the art Six kinds of products such as cellulosic ethanol, lignin, biogas, bio-feritlizer, animal feeding-stuff containing somatic protein, CO2 are produced simultaneously, realize raw material Full constituent makes full use of.
2. stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, the agricultural wastes such as stalk in production process The yield of raw material stalk is greatly improved using thermal and hydric environment processing mode, the generation of harmful toxic matter is reduced.
Stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, and the useless mash of distillation directly carries out anaerobism hair Ferment treatment, biogas slurry carries out separating production lignin after anaerobism, improves the operability of lignin separation.
Stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, and using two-stage UF membrane, technology will detest Oxygen sewage carries out advanced treating, reaches boiler soft water requirement and produces biomass fertilizers.
Stalk of the invention produces the agricultural wastes such as cellulosic ethanol and five kinds of methods of byproduct, stalk in this technique In produce cellulosic ethanol, lignin, protein feed, bio-feritlizer, biogas, six kinds of products such as liquid CO 2 simultaneously.
Stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, and the enzymolysis liquid glucose for being produced is not required to carry out Separation of solid and liquid, can directly be fermented.
Stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, and the useless wine with dregs of produced distillation carries out anaerobism After fermentation, lignin, bio-feritlizer, technique Water circulation can be separated.
Stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, and the fermentation later stage realizes distillery yeast Separate production animal feeding-stuff containing somatic protein.
Stalk of the invention produces cellulosic ethanol and five kinds of methods of byproduct, in process of production, the agricultural such as stalk Discarded object uses thermal and hydric environment processing method.
Brief description of the drawings:
Accompanying drawing 1 is structural representation of the invention.
Specific embodiment:
Embodiment 1:
The method that stalk produces cellulosic ethanol, comprises the following steps:(1)The treatment of stalk early stage and thermal and hydric environment pretreatment;(2) Batch mixing and enzymatic saccharification are processed;(3)Fermentation purification processes;(4)Ferment the CO for producing2Treatment;(5)The useless wine with dregs treatment of distillation;(6)Ferment Mother's fermentation after fermentation mash separating thallus albumen, described fermentation purification processes process is:After through enzymolysis, liquid glucose is cooled to 32 20% yeast is accessed after DEG C carries out alcoholic fermentation, and fermentation time 70h carries out temperature control, hair is taken away using frozen water in fermentation process The fermentation fever that ferment is produced, control fermentation temperature is in 30-35 DEG C, residual sugar<0.3% is considered as fermentation ends, terminates the alcohol hair of fermentation Ferment mash delivers to distilling apparatus and carries out distilation and obtain cellulosic ethanol after precipitating 48h yeast separations, and distillation is using double Thick column differential-pressure distillation device carries out distillation procedure, and operation temperature is 103-108 DEG C of thick tower, 0.06-0.15mpa of tower top pressure, 85-95 DEG C of combined column temperature, tower top pressure-0.05-- 0.08mpa;Smart 120-128 DEG C of tower temperature degree, tower top pressure 0.06- 0.15mpa。
Embodiment 2:
The method that described stalk produces cellulosic ethanol, described stalk early stage treatment and thermal and hydric environment preprocessing process are: Enter alcohol production device area after maize straw is collected into bundling, stacking storage, conveying crushing, dedusting removal of impurities, drying, briquetting, The briquetting stalk of 15% moisture delivers to hot water environment formula bolt after Water spray pre-soaking, washing after moisture reaches 60% in device area In plug flow reactor, 1.3-1.8mpa steam is passed through in the reactor, ton stalk needs 0.2-0.5t of steam, stop 12- 15min is reacted.
Embodiment 3:
The method that stalk described in embodiment 1 or 2 produces cellulosic ethanol, described batch mixing and enzymatic saccharification processing procedure be: Stalk material delivers to enzymatic vessel by blender after being processed through thermal and hydric environment, added in blender liquid NaOH adjust PH to 4.8-5.0,2-5kg/ tons of stalk of liquid NaOH additions, cellulase 10kg/t stalks carry out enzymatic saccharification, controlled enzymatic hydrolysis temperature 50-55 DEG C of degree.
Embodiment 4:
The carbon dioxide of the method by-product of the stalk production cellulosic ethanol described in embodiment 1 or 2 or 3, described fermentation is produced CO2Processing procedure is:Ferment the CO for producing2Dissolved organic matter gas, the CO2 gas after purification are removed by Water spray washing Body carries out spray washing and removes insoluble grease through 0.06% liquor potassic permanganate, and first is crossed by the gas after two-step purification Boosted into carbon-dioxide gas compressor after water distributing can point water, after boosting to 3.0MPa, proceeding through the first cooler, second Water distributing can and drying bed are further dehydrated, and the CO2 after dehydration enters adsorbent bed after the second cooler is cooled down, and carries out removing impurities matter Treatment, adsorbent bed is by eight kinds of different types of adsorbents layered arrangement in proportion, it is ensured that efficient impurity absorption curve, is pure One of key equipment of change, the forecooler and pressure of -30 DEG C of CO2 entrance after purification are liquefied for the liquefier of 3.0MPa, Low-temperature receiver is liquefied ammonia;Liquefier liquid CO 2 out enters in finished pot.
Embodiment 5:
The mycoprotein of the method by-product of one of embodiment 1-4 described stalk production cellulosic ethanol, described yeast fermentation After fermentation mash processing procedure is:Stop fermentation stirring fermentation liquid after yeast fermentation to staticly settle 48h, taken according to tank body middle part It is considered as sedimentation when distiller's yeast cell number is less than 2,000,000 plants/ml in sample chemical examination karusen flow container to terminate, centrifuge obtains yeast after separating Animal feeding-stuff containing somatic protein, through being dried to obtain commodity mycoprotein.
Embodiment 6:
The method that cellulosic ethanol is produced according to one of embodiment 1-4 described stalk, described stalk early stage treatment and hydro-thermal Environment preprocessing process is:Maize straw is collected into bundling, stacking storage, conveying crushing, dedusting removal of impurities, drying, briquetting granulation Afterwards, in plug flow reactor, steam is added, is pre-processed by thermal and hydric environment device, control steam explosion pressure 1.3- 1.8Mpa。
Embodiment 7:
The method that stalk according to one of embodiment 1-4 or embodiment 6 produces cellulosic ethanol, described batch mixing and enzyme Solving saccharification processing procedure is:Stalk material delivers to enzymatic vessel by blender after being processed through thermal and hydric environment, and work is pressed in blender Skill requirement addition liquid caustic soda, cellulase carry out enzymatic saccharification, 50-55 DEG C of controlled enzymatic hydrolysis temperature.
Embodiment 8:
The method that stalk according to one of embodiment 1-4 or embodiment 6 or 7 produces cellulosic ethanol, described fermentation Purification processes process is:Accessing yeast after through enzymolysis, after liquid glucose is cooled into 32 DEG C carries out alcoholic fermentation, and ferment after ripening mash Distilation is carried out by delivering to distilling apparatus after yeast separation and obtains cellulosic ethanol.
Embodiment 9:
The method that stalk according to one of embodiment 1-4 or embodiment 6 or 7 or 8 produces cellulosic ethanol, described hair The CO that ferment is produced2Processing procedure is:Ferment the CO for producing2Washed by washing, potassium, compressed, be dehydrated, freezing production liquid CO2

Claims (5)

1. a kind of method that stalk produces cellulosic ethanol, it is characterized in that:Comprise the following steps:(1)The treatment of stalk early stage and water Thermal environment is pre-processed;(2)Batch mixing and enzymatic saccharification are processed;(3)Fermentation purification processes;(4)Ferment the CO for producing2Treatment;(5)Steam Evaporate useless wine with dregs treatment;(6)Yeast ferments after fermentation mash separating thallus albumen, and described fermentation purification processes process is:Through enzymolysis Afterwards, liquid glucose is cooled to after 32 DEG C and accesses 20% yeast and carry out alcoholic fermentation, fermentation time 70h enters trip temperature control in fermentation process System, the fermentation fever that fermentation is produced is taken away using frozen water, and control fermentation temperature is in 30-35 DEG C, residual sugar<0.3% is considered as fermentation ends, Terminate the alcoholic fermented liquor of fermentation after precipitating 48h yeast separations, deliver to distilling apparatus and carry out distilation and obtain fiber Plain ethanol, distillation carries out distillation procedure using double thick column differential-pressure distillation devices, and operation temperature is 103-108 DEG C of thick tower, tower top pressure 0.06-0.15mpa of power, 85-95 DEG C of combined column temperature, tower top pressure-0.05-- 0.08mpa;Smart tower temperature degree 120-128 DEG C, 0.06-0.15mpa of tower top pressure.
2. the method that stalk according to claim 1 produces cellulosic ethanol, it is characterized in that:Described stalk early stage treatment And thermal and hydric environment preprocessing process is:Maize straw is collected into bundling, stacking storage, conveying crushing, dedusting removal of impurities, drying, pressure Enter alcohol production device area after block, the briquetting stalk of 15% moisture moisture after Water spray pre-soaking, washing reaches in device area Delivered to after to 60% in hot water environment formula plug flow reactor, 1.3-1.8mpa steam is passed through in the reactor, ton stalk needs 0.2-0.5t of steam, stops 12-15min and is reacted.
3. the method that stalk according to claim 1 and 2 produces cellulosic ethanol, it is characterized in that:Described batch mixing and enzyme Solving saccharification processing procedure is:Stalk material delivers to enzymatic vessel by blender after being processed through thermal and hydric environment, is added in blender Liquid NaOH adjusts PH to 4.8-5.0, and 2-5kg/ tons of stalk of liquid NaOH additions, cellulase 10kg/t stalks carry out enzyme Solution saccharification, 50-55 DEG C of controlled enzymatic hydrolysis temperature.
4. the stalk described in a kind of claim 1 produces the carbon dioxide of the method by-product of cellulosic ethanol, it is characterized in that:It is described Fermentation produce CO2Processing procedure is:Ferment the CO for producing2Dissolved organic matter gas, purification are removed by Water spray washing CO2 gases afterwards carry out spray washing and remove insoluble grease through 0.06% liquor potassic permanganate, by the gas after two-step purification Body is boosted after crossing the first water distributing can point water into carbon-dioxide gas compressor, cold proceeding through first after boosting to 3.0MPa But device, the second water distributing can and drying bed are further dehydrated, and the CO2 after dehydration enters adsorbent bed after the second cooler is cooled down, Carry out de- impurity treatment, adsorbent bed is by eight kinds of different types of adsorbents layered arrangement in proportion, it is ensured that efficient impurity is inhaled Attached curve, is one of key equipment of purifying, and the forecooler and pressure of -30 DEG C of CO2 entrance after purification are the liquefaction of 3.0MPa Device is liquefied, and low-temperature receiver is liquefied ammonia;Liquefier liquid CO 2 out enters in finished pot.
5. a kind of one of claim 1-3 described stalk produces the mycoprotein of the method by-product of cellulosic ethanol, its feature It is:Described yeast fermentation after fermentation mash processing procedure is:Stop fermentation stirring fermentation liquid after yeast fermentation to staticly settle 48h, it is considered as sedimentation and terminates when being less than 2,000,000 plants/ml according to distiller's yeast cell number in the sample examination karusen flow container of tank body middle part, from Scheming obtains yeast animal feeding-stuff containing somatic protein after separating, through being dried to obtain commodity mycoprotein.
CN201710181164.2A 2017-03-24 2017-03-24 The method that stalk produces cellulosic ethanol Pending CN106834361A (en)

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CN110846345A (en) * 2019-12-06 2020-02-28 白博 Production process of cotton straw fulvic acid and cellulosic ethanol
CN114560557A (en) * 2022-01-25 2022-05-31 太原市润民环保节能有限公司 Efficient composite carbon source for denitrification treatment of wastewater

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108841912A (en) * 2018-06-20 2018-11-20 冯国青 A kind of technique preparing and separate biological butanol and bio-ethanol
CN110846345A (en) * 2019-12-06 2020-02-28 白博 Production process of cotton straw fulvic acid and cellulosic ethanol
CN114560557A (en) * 2022-01-25 2022-05-31 太原市润民环保节能有限公司 Efficient composite carbon source for denitrification treatment of wastewater

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