CN106834122A - Biocontrol bacterial strain X1 fermentation mediums - Google Patents
Biocontrol bacterial strain X1 fermentation mediums Download PDFInfo
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- CN106834122A CN106834122A CN201510875955.6A CN201510875955A CN106834122A CN 106834122 A CN106834122 A CN 106834122A CN 201510875955 A CN201510875955 A CN 201510875955A CN 106834122 A CN106834122 A CN 106834122A
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- bacterial strain
- biocontrol bacterial
- fermentation mediums
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Abstract
The present invention discloses a kind of biocontrol bacterial strain X1 fermentation mediums, including following composition：Glucose 1-2%, yeast extract 0.3-0.6%, starch 0.1-0.3%, disodium hydrogen phosphate 0.2-0.5%, sodium carbonate 0.1-0.4%, magnesium sulfate 0.04-0.06%, calcium carbonate 0.05-0.2%, manganese dioxide 0.02-0.1%, potassium phosphate 0.03-0.08%, plus distilled water is to 1000 milliliters.Simple and easy to get and low cost of the invention, all the components can dissolve, and post-partum period treatment of making a living facilitates feasible, while for large-scale industrial production provides technical basis.
Description
Technical field
The invention belongs to microbial technology field, and in particular to a kind of biocontrol bacterial strain X1 fermentation mediums.
Background technology
Antagonistic ability is strong and excellent species of has a broad antifungal spectrum are keys that Chinese herbal medicine soil-borne disease biological control research and microbial bacterial agent are developed, and suitable production method and condition of culture is then the guarantee for playing excellent species performance.After screening obtains high-efficiency broad spectrum biocontrol bacterial strain, to be finally reached industry development purpose, it is necessary to carry out liquid deep layer fermenting research to purpose bacterial strain, including the optimization and the determination of scale up test technique for being adapted to the Screening of Media, liquid fermentation condition of industrialized production.
Optimization culture or fermentation results in laboratory research is transferred in plant-scale fermentation production process, guarantee scale amplify after can in large-scale industrial production same successful effect, the research or the intermediate experiment of plant layout of the fermentation condition for then needing to carry out more larger, to verify the zymotechnique in the laboratory research stage, the fermenting and producing of plant layout then can be just amplified to.
The content of the invention
The technical problems to be solved by the invention are to be directed to above-mentioned the deficiencies in the prior art, there is provided a kind of biocontrol bacterial strain X1 fermentation mediums, it provides experiment basis and foundation to carry out the industrialized production of the bacterial strain.
The technical solution adopted in the present invention is：A kind of biocontrol bacterial strain X1 fermentation mediums, including following composition：Glucose 1-2%, yeast extract 0.3-0.6%, starch 0.1-0.3%, disodium hydrogen phosphate 0.2-0.5%, sodium carbonate 0.1-0.4%, magnesium sulfate 0.04-0.06%, calcium carbonate 0.05-0.2%, manganese dioxide 0.02-0.1%, potassium phosphate 0.03-0.08%, plus distilled water is to 1000 milliliters.
Preferably, glucose 1.5%, yeast extract 0.5%, starch 0.2%, disodium hydrogen phosphate 0.3%, sodium carbonate 0.4%, magnesium sulfate 0.04%, calcium carbonate 0.05%, manganese dioxide 0.1%, potassium phosphate 0.04%.
The beneficial effects of the present invention are：Simple and easy to get and low cost of the invention, all the components can dissolve, and post-partum period treatment of making a living facilitates feasible, while for large-scale industrial production provides technical basis.
Specific embodiment
The present invention is described in further detail below in conjunction with specific embodiment.
Embodiment：The present invention includes following composition：Glucose 1-2%, yeast extract 0.3-0.6%, starch 0.1-0.3%, disodium hydrogen phosphate 0.2-0.5%, sodium carbonate 0.1-0.4%, magnesium sulfate 0.04-0.06%, calcium carbonate 0.05-0.2%, manganese dioxide 0.02-0.1%, potassium phosphate 0.03-0.08%, plus distilled water is to 1000 milliliters.Embodiment 1：Glucose 1.5% is taken, yeast extract 0.5%, starch 0.2%, disodium hydrogen phosphate 0.3%, sodium carbonate 0.4%, magnesium sulfate 0.04%, calcium carbonate 0.05%, manganese dioxide 0.1%, potassium phosphate 0.04% adds 1000ml distilled water.
Simple and easy to get and low cost of the invention, all the components can dissolve, and post-partum period treatment of making a living facilitates feasible, while for large-scale industrial production provides technical basis.
Claims (2)
1. a kind of biocontrol bacterial strain X1 fermentation mediums, it is characterised in that：Including following composition：Glucose 1-2%, yeast extract 0.3-0.6%, starch 0.1-0.3%, disodium hydrogen phosphate 0.2-0.5%, sodium carbonate 0.1-0.4%, magnesium sulfate 0.04-0.06%, calcium carbonate 0.05-0.2%, manganese dioxide 0.02-0.1%, potassium phosphate 0.03-0.08%, plus distilled water is to 1000 milliliters.
2. biocontrol bacterial strain X1 fermentation mediums according to claim 1, it is characterised in that：Glucose 1.5%, yeast extract 0.5%, starch 0.2%, disodium hydrogen phosphate 0.3%, sodium carbonate 0.4%, magnesium sulfate 0.04%, calcium carbonate 0.05%, manganese dioxide 0.1%, potassium phosphate 0.04%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510875955.6A CN106834122A (en) | 2015-12-03 | 2015-12-03 | Biocontrol bacterial strain X1 fermentation mediums |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510875955.6A CN106834122A (en) | 2015-12-03 | 2015-12-03 | Biocontrol bacterial strain X1 fermentation mediums |
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| CN106834122A true CN106834122A (en) | 2017-06-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510875955.6A Pending CN106834122A (en) | 2015-12-03 | 2015-12-03 | Biocontrol bacterial strain X1 fermentation mediums |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6280722B1 (en) * | 1996-08-30 | 2001-08-28 | Auburn University | Antifungal Bacillus thuringiensis strains |
| CN105039230A (en) * | 2015-09-18 | 2015-11-11 | 黑龙江省科学院微生物研究所 | Biocontrol strain X1 fermentation medium and small-scale fermentation technology |
| CN105087449A (en) * | 2015-09-18 | 2015-11-25 | 黑龙江省科学院微生物研究所 | Pilot-scale fermentation process of biocontrol strain X1 |
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2015
- 2015-12-03 CN CN201510875955.6A patent/CN106834122A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6280722B1 (en) * | 1996-08-30 | 2001-08-28 | Auburn University | Antifungal Bacillus thuringiensis strains |
| CN105039230A (en) * | 2015-09-18 | 2015-11-11 | 黑龙江省科学院微生物研究所 | Biocontrol strain X1 fermentation medium and small-scale fermentation technology |
| CN105087449A (en) * | 2015-09-18 | 2015-11-25 | 黑龙江省科学院微生物研究所 | Pilot-scale fermentation process of biocontrol strain X1 |
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Application publication date: 20170613 |