CN106822275A - A kind of composition for significantly improving relieving alcoholism and protecting liver activity, its preparation method and application - Google Patents

A kind of composition for significantly improving relieving alcoholism and protecting liver activity, its preparation method and application Download PDF

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CN106822275A
CN106822275A CN201710076838.2A CN201710076838A CN106822275A CN 106822275 A CN106822275 A CN 106822275A CN 201710076838 A CN201710076838 A CN 201710076838A CN 106822275 A CN106822275 A CN 106822275A
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parts
composition
extract
royal jelly
root
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CN106822275B (en
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龙祥
张伟平
洪早梅
潘丽婷
卢阳辉
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Yunnan De Caitang Biological Medicine Science And Technology Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/56—Materials from animals other than mammals
    • A61K35/63—Arthropods
    • A61K35/64—Insects, e.g. bees, wasps or fleas
    • A61K35/644—Beeswax; Propolis; Royal jelly; Honey
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/72—Rhamnaceae (Buckthorn family), e.g. buckthorn, chewstick or umbrella-tree
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps

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Abstract

The active composition of relieving alcoholism and protecting liver, its preparation method and application are significantly improved the invention provides a kind of.The composition is made up of royal jelly extract, the root of kudzu vine, hoveniae semoveniae semen.Wherein, the root of kudzu vine, hoveniae semoveniae semen are mixed using 80% alcohol heat reflux and extracted, extract obtained general flavone content >=30%；Royal jelly extract is obtained by raw material of royal jelly through extracting, and royal jelly acid content is between 10~50%；The root of kudzu vine, hoveniae semoveniae semen mixed extract and royal jelly extract addition quick-release, controlled-release solid dispersible carrier, composition is obtained using solid dispersion technology.Experiment shows：Composition provided by the present invention can significantly extend mouse because of the Sleep latency caused by ethanol, curtail sleep, significantly reduce concentration of alcohol in rat blood, reduce because of the influence of ethanol induced mice body weight and liver GSH, MDA, TG, ADH.It is significant to relieving alcoholism and protecting liver medicine, health food, feature ordinary food, the exploitation of special diet food research.

Description

A kind of composition for significantly improving relieving alcoholism and protecting liver activity, its preparation method and application
Technical field
The present invention relates to a kind of composition for significantly improving relieving alcoholism and protecting liver activity.More particularly to one kind is extracted by royal jelly Thing, the root of kudzu vine, hoveniae semoveniae semen compounding, mixing extraction, extract and royal jelly are carried out using 80% alcohol heat reflux to the root of kudzu vine, hoveniae semoveniae semen The composition that extract is prepared using solid dispersion technology.
Background technology
Low alcohol consumption can invigorate blood circulation, dredging collateral, cold dispelling, excessive consumption of alcohol can then produce " alcoholism " (being commonly called as drunk).Medically Alcoholism is divided into two kinds of acute alcoholism and chronic alcoholism.Wherein, after acute alcoholism refers to excessive consumption of alcohol, Bring Nausea and vomiting, dizziness, delirious speech, restless, stupor, gatism, respiration inhibition even dead to patient in the short period Clinical symptoms；Chronic alcoholism refers to that due to long-term excessive consumption of alcohol the ethanol into human body can not be digested absorption, with blood Into brain, neuronal cell film is destroyed, weaken central nervous system, and by activating inhibiting nerve original and suppressing activity Neuron causes slow brain activity, neuronal cell death, severe patient to cause alcoholic cirrhosis and some cancers (such as oral cavity Cancer, tongue cancer, liver cancer).In recent years since, alcohol consumption colony of China is growing day by day, it has also become factors leading to social instability and enjoy pass Note.At present, the disintoxicating product that market occurs is more based on chemicals, Chinese patent medicine preparation, while relieving the effect of alcohol, can be different degrees of Liver, renal metabolism is aggravated to bear and produce different degrees of injury.
The root of kudzu vine, hoveniae semoveniae semen are studied first recorded in China as traditional integration of drinking and medicinal herbs medicinal material, its antialcoholism function《Prescriptions worth thousand gold》、《This Careless detailed outline》Etc. medical science monumental work.Modern pharmacy, pharmacology, pharmacodynamics, clinical medicine are to the root of kudzu vine, the research of hoveniae semoveniae semen antialcoholism action Document is more, and the product as development of raw materials listing of relieving the effect of alcohol is also of common occurrence.In addition, " root of kudzu vine relieves the effect of alcohol granular tea and its preparation side Method (Patent publication No：CN105767355A) " disclose a kind of root of kudzu vine to relieve the effect of alcohol granular tea, by 56~71 parts of the root of kudzu vine, small wheat kernel 10 ~15 parts, 4~9 parts of hoveniae semoveniae semen, 5~8 parts of mulberries leaf, 1~3 part of flower of kudzuvine, 5~7 parts of Poria cocos composition；" a kind of protect liver, alleviation are after drinking Uncomfortable solid beverage and preparation method thereof (Patent publication No：CN106261447A) " disclose a kind of protect liver, alleviate after drinking not Suitable solid beverage, by 20~40 parts of hoveniae semoveniae semen, 20~40 parts of the root of kudzu vine, 15~35 parts of sea-buckthorn, 10~30 parts of Radix Glycyrrhizae, dark plum 30~ 90 parts, 1~6 part of composition of rhizoma zingiberis；It is " a kind of that there is chewable tablets of protective effect and preparation method thereof (patent to alcoholic liver injury Publication No.：CN106038733A) " disclose a kind of chewable tablets to alcoholic liver injury with protective effect, by rheum officinale 15~ 30 parts, 9~30 parts of hoveniae semoveniae semen, 3~6 parts of Antrodia camphorata, 15~30 parts of the Radix Astragali, 9~15 parts of root of kudzu vine composition.
Royal jelly is the secretion of the young worker bee pharynx gland of cultivation larva in honeycomb, is supplied with that queen bee will be become The food of larva, is also the lifelong food of queen bee.Used as traditional medicine-food two-purpose medicinal material, the pharmacological action of modern study confirmation is to change Kind nutrition, improve immune, auxiliary prevention cardiovascular and cerebrovascular disease, anti-inflammation anti-cancer, anti-ageing wait for a long time.
It is contemplated that with the royal jelly extract of traditional medicine-food two-purpose, the root of kudzu vine, hoveniae semoveniae semen as combination raw materials, by this hair The technical schemes such as bright specific composition ratio, extraction process, functional component control, solid dispersion are implemented, and prepare efficient relieving alcoholism and protecting liver Composition, is research and development relieving alcoholism and protecting liver medicine, health food, feature ordinary food, special medicine food, special diet Food provides combination raw materials or preparation.
The content of the invention：
The first object of the present invention is to provide a kind of composition for significantly improving relieving alcoholism and protecting liver activity and preparation method thereof； Second purpose is to provide the application of said composition.
The first object of the present invention is realized using following technical proposals：
First, the present invention provides a kind of composition for significantly improving relieving alcoholism and protecting liver activity, and the composition is by parts by weight 10~19 parts of royal jelly extract, the root of kudzu vine of 291~763 parts of parts by weight, the hoveniae semoveniae semen group of 171~763 parts of parts by weight Into；
Preferably, the composition by 12~18 parts of parts by weight royal jelly extract, 350~650 parts of parts by weight The root of kudzu vine, 200~500 parts of parts by weight hoveniae semoveniae semen composition；
Further preferably, the composition by 13~17 parts of parts by weight royal jelly extract, parts by weight 40~600 The root of kudzu vine of part, the hoveniae semoveniae semen composition of 250~450 parts of parts by weight；
Most preferably, the composition by 14~16 parts of parts by weight royal jelly extract, 450~550 parts of parts by weight The root of kudzu vine, 300~400 parts of parts by weight hoveniae semoveniae semen composition.
Secondly, it is raw material that royal jelly extract system of the present invention uses royal jelly, is obtained using prior art extracting.Its In, royal jelly acid content is 10%~50%；Preferably 20%~40%；More preferably 30%~40%.
Thirdly, the root of kudzu vine of the present invention, hoveniae semoveniae semen system are mixed using 80% alcohol heat reflux and extracted.Specially：By Pueraria lobota Root, hoveniae semoveniae semen co-grinding soak 12 hours into 20-40 mesh meal, 80% ethanol wet of addition, and addition solid-liquid ratio is 1:10th, it is dense Spend for 80% alcohol reflux is extracted three times, extract 1.5 hours every time, extract temperature control between 75~80 DEG C, collect extract solution warp Concentrated under reduced pressure, dry mixed extract.Wherein, general flavone content >=30%.
Finally, royal jelly extract of the present invention, root of kudzu vine hoveniae semoveniae semen mixed extract are mixed, addition absolute ethyl alcohol, One or more organic solvents dissolving in ethanol, acetone, chloroform.Addition Macrogol 4000, Macrogol 6000, PVP Anhydrous second is melted or used to one or more in K30, poloxamer-188, xylitol, sorbierite, citric acid as carrier Royal jelly extract is added to after one or more organic solvents dissolving in alcohol, ethanol, acetone, chloroform to mix with root of kudzu vine hoveniae semoveniae semen In extract solution, it is sufficiently mixed uniformly, boils off solvent and obtain the co-precipitation solid dispersions that extract is mixed in the carrier, Through being drying to obtain composition of the present invention.
Preferably, dissolving royal jelly extract and the organic solvent solid-liquid ratio of root of kudzu vine hoveniae semoveniae semen mixed extract are 1:1~ 10, preferably 2~8, more preferably 3~6, most preferably 4~5.
Preferably, carrier dissolving is using heating and melting or addition organic solvent dissolving two ways.Organic solvent dissolving side Formula solid-liquid ratio is 1:1~10, preferably 2~8, more preferably 3~6, most preferably 4~5.
The second object of the present invention is realized using following technical proposals：
First, composition of the present invention, with relieving alcoholism and protecting livers, liver-protecting function/effect, can be applied to medicine, health care food Product, feature ordinary food, special medicine food, the exploitation of special diet food research.
Secondly, composition of the present invention can be added or common without medicine, health food, feature as needed Food, special medicine food, the acceptable auxiliary material of special diet food, are further made granule, tablet, hard capsule, drop The solid orally ingestibles such as ball.
Specific embodiment
Further detailed description is done to the present invention with reference to specific embodiment, it will be appreciated by those skilled in the art that These embodiments are merely to illustrate the present invention, but embodiments of the present invention not limited to this.
Embodiment 1：It is prepared by the root of kudzu vine, raisin tree seed extract
The root of kudzu vine of the present invention, hoveniae semoveniae semen system mix extraction using 80% alcohol heat reflux.Specific method is as follows：Take Pueraria lobota Root, each 100g of hoveniae semoveniae semen crude drug, co-grinding cross 40 mesh sieves, addition 80% appropriate amount of ethanol wetting immersion 12 hours, additive Liquor ratio is 1:10th, concentration be 80% alcohol reflux extract three times, every time extract 1.5 hours, extract temperature control between 75~80 DEG C, Extract solution is collected through concentrated under reduced pressure, dry mixed extract 19.92g.Using high effective liquid chromatography for measuring general flavone content, General flavone content is 54.23% in measuring extract.
The screening of alcohol heat reflux technique for extracting mixed condition of the present invention：
The root of kudzu vine, each 50g of hoveniae semoveniae semen crude drug are taken, co-grinding crosses 40 mesh sieves, addition 80% appropriate amount of ethanol wetting immersion 12 Hour, addition solid-liquid ratio is 1:10th, concentration is that 80% alcohol reflux is extracted three times, is extracted 1.5 hours every time, extracts temperature control 75 Between~80 DEG C, extract solution is collected through concentrated under reduced pressure, dry mixed extract.Using high effective liquid chromatography for measuring general flavone Content.
Hereinafter investigate experiment and above-mentioned flow implementation is pressed using same batch medicine, consumption is with each 50g of crude drug.
A. the influence of concentration of alcohol
Influence in view of concentration of alcohol to general flavone yield, thus use 20%, 30%, 40%, 50%, 60%, 70%, 80%th, 90%, 100% ethanol, 9 levels are carried out concentration of alcohol process optimization and are investigated, (the temperature in the case where other conditions are constant 78 DEG C of degree, extraction time 1.5h, solid-liquid ratio 1:10), with general flavone content (general flavone content in extract medicinal extract) for investigation refers to Mark, the results are shown in Table 1.
The different ethanol concentration of table 1 influences result of the test to general flavone yield
By the result in table 1, concentration of alcohol makes curve map to general flavone yield, specific as shown in Figure 1.Can be with from Fig. 1 Find out：In the case where other conditions are constant, when concentration of alcohol is less than 50%, general flavone yield has no significant change；Work as second During determining alcohol >=50%, with the increase of concentration, the yield of general flavone also rises therewith；When concentration of alcohol is more than 90%, can be with Find out that yield begins to decline.Therefore, optimum extraction concentration of alcohol is 80%.
B. the influence of temperature
Influence in view of Extracting temperature to general flavone yield, with reference to industrialized production security, using 20,30,40, 50th, 60,70,80,90 DEG C of 8 levels are carried out Extracting temperature process optimization and are investigated, and in the case where other conditions are constant, (ethanol is dense Degree 80%, extraction time 1.5h, solid-liquid ratio 1:10), with general flavone content (general flavone content in extract medicinal extract) for investigation refers to Mark, the results are shown in Table 2.
The Extracting temperature of table 2 influences result of study to general flavone yield
Temperature in table 2 is made into curve map to general flavone yield, it is specific as shown in Figure 2.As can be seen from Figure 2：At other In the case that condition is constant, when temperature is less than 30 DEG C, general flavone is not extracted；When temperature is higher than 30 DEG C, with temperature Increase, the yield of general flavone also rises therewith；When temperature is more than 80 DEG C, it can be seen that yield starts on a declining curve.Cause This, optimum extraction temperature is 75~80 DEG C.
C. the influence of extraction time
Influence in view of extraction time to general flavone yield, thus use 30,40,50,60,70,80,90,100,110, 120min10 level is carried out extraction time process optimization and is investigated, (concentration of alcohol 80%, temperature in the case where other conditions are constant 78 DEG C of degree, solid-liquid ratio 1:10), as inspection target, 3 be the results are shown in Table with general flavone content (general flavone content in extract medicinal extract).
The extraction time of table 3 influences result of study to general flavone yield
Extraction time in table 3 is made into curve map to general flavone yield, it is specific as shown in Figure 3.As can be seen from Figure 3： In the case where other conditions are constant, when extraction time 90min is less than, general flavone yield increases with the increase of extraction time Greatly；When extraction time 90min is more than, there is obvious downward trend with the increase of extraction time in general flavone yield.Therefore, The optimum extraction time is 90min.
D. the influence of solid-liquid ratio
The influence to general flavone yield and Cost Problems in view of solid-liquid ratio, therefore it is 1 to use solid-liquid ratio:5、1:6、1:7、1: 8、1:9、1:10、1:11、1:12、1:13 amount to 9 levels carries out the investigation of solid-liquid ratio process optimization, in the feelings that other conditions are constant Under condition (concentration of alcohol 80%, extraction time 1.5h, 78 DEG C of temperature), with general flavone content (general flavone content in extract medicinal extract) It is inspection target, the results are shown in Table 4.
The solid-liquid ratio of table 4 influences result of study to general flavone yield
Solid-liquid ratio in table 4 is made into column diagram on the influence of general flavone yield, it is specific as shown in Figure 4.As can be seen from Figure 4： In the case where other conditions are constant, when solid-liquid ratio is less than 1:When 10, the yield of general flavone increases with the increase of quantity of solvent；When Solid-liquid ratio is more than 1:When 10, the yield of general flavone is not changed significantly, illustrates that now general flavone has been transferred completely, is section About cost, it is 1 to select optimal solid-liquid ratio:10.
Brief description of the drawings
The concentration of alcohol of Fig. 1 embodiment of the present invention 1 is to general flavone yield influence curve figure
The Extracting temperature of Fig. 2 embodiment of the present invention 1 is to general flavone yield influence curve figure
The extraction time of Fig. 3 embodiment of the present invention 1 is to general flavone yield influence curve figure
Fig. 4 embodiment of the present invention 1 extracts solid-liquid ratio influences column diagram to general flavone yield
Embodiment 2：Royal jelly extract prepares
Directly purchase the royal jelly extract (remarks that royal jelly acid indicates content >=30%：This royal jelly extract is by royal jelly The royal jelly of acid content >=1.8% is extracted and is obtained), it is 33.48% to use high performance liquid chromatography self-inspection royal jelly acid content, as The present composition is prepared and used.
Embodiment 3：Composition prepares 1
A. root of kudzu vine 600g, hoveniae semoveniae semen 450g are taken and mixes extraction by the alcohol heat reflux of embodiment 1, obtain extract 105g；Take reality The royal jelly extract 18g of the preparation of example 2 is applied, 80 mesh sieves is crossed standby.
B. royal jelly extract, root of kudzu vine hoveniae semoveniae semen mixed extract are mixed, adds 10 times of anhydrous alcohol solutions of amount, system Into mixed liquor.Addition Macrogol 4000, PVP K30, xylitol as carrier, using being added to after 8 times of acetone solutions of amount In above-mentioned suspension, it is sufficiently mixed uniformly, boils off solvent and obtain the co-precipitation solid dispersions that extract is mixed in the carrier, Through dry composition 120g.
Embodiment 4：Composition prepares 2
A. root of kudzu vine 550g, hoveniae semoveniae semen 400g are taken and mixes extraction by the alcohol heat reflux of embodiment 1, obtain extract 95g；Take implementation The royal jelly extract 16g that example 2 prepares, crosses 100 mesh sieves standby.
B. royal jelly extract, root of kudzu vine hoveniae semoveniae semen mixed extract are mixed, adds 8 times of ethanol dissolvings of amount, be made mixed Suspension.Addition Macrogol 4000, PVP K30, xylitol as carrier, using being added to after 8 times of anhydrous alcohol solutions of amount In above-mentioned suspension, it is sufficiently mixed uniformly, boils off solvent and obtain the co-precipitation solid dispersions that extract is mixed in the carrier, Through dry composition 109g.
Embodiment 5:Composition prepares 3
A. root of kudzu vine 450g, hoveniae semoveniae semen 300g are taken and mixes extraction by the alcohol heat reflux of embodiment 1, obtain extract 75g；Take implementation The royal jelly extract 14g that example 2 prepares, crosses 80 mesh sieves standby.
B. royal jelly extract, root of kudzu vine hoveniae semoveniae semen mixed extract are mixed, adds 6 times of acetone solutions of amount, be made mixed Suspension.Addition PVP K30, poloxamer-188, sorbierite are added to above-mentioned as carrier after being dissolved using 5 times of chloroforms of amount In suspension, it is sufficiently mixed uniformly, boils off solvent and obtain the co-precipitation solid dispersions that extract is mixed in the carrier, through dry It is dry to obtain composition 87g.
Embodiment 6：Composition prepares 4
A. root of kudzu vine 5000g, hoveniae semoveniae semen 3500g are taken and mixes extraction according to the alcohol heat reflux described in embodiment 1, must extracted Thing 847g；The royal jelly extract 150g that Example 2 prepares, crosses 80 mesh sieves standby.
B. royal jelly extract, root of kudzu vine hoveniae semoveniae semen mixed extract are mixed, adds the chloroform dissolving of 5 times of amounts to be made suspension Liquid.Macrogol 4000, poloxamer-188, sorbierite, citric acid are added as carrier, after 4 times of acetone solutions of amount Add in above-mentioned suspension, be sufficiently mixed uniformly, boil off solvent and obtain the co-precipitation solid point that extract is mixed in the carrier A prose style free from parallelism, through dry composition 977g.
Embodiment 7：Preparation prepares 1
Composition 200g prepared by Example 5, adds appropriate Icing Sugar, dextrin, lactose, mannitol, through granulation, dry Granule with relieving alcoholism and protecting liver activity.
Embodiment 8：Preparation prepares 2
Composition 200g prepared by Example 5, addition appropriate amount of starch, Icing Sugar, dextrin, PVP, mucialga of arabic gummy, warp Mixing, granulation, compressing tablet are made the tablet with relieving alcoholism and protecting liver activity.
Embodiment 9：Preparation prepares 3
Composition 200g prepared by Example 5, addition suitable amount of sucrose, lactose, microcrystalline cellulose, silica, talcum Powder, magnesium stearate, blended, granulation, filling are prepared into the hard shell capsules with relieving alcoholism and protecting liver activity.
Embodiment 10：Preparation prepares 4
Composition 200g prepared by Example 5, add appropriate Macrogol 4000, Macrogol 6000, odium stearate, Glycerin gelatine, polyoxyl 40 stearate, blended, dissolving, pill are prepared into the dripping pill with relieving alcoholism and protecting liver activity.
In order to further be illustrated to present composition technique effect, the present invention chooses the honeybee that embodiment 2 prepares Royal jelly extract, general flavone content be 54.0% kudzu root extract, general flavone content be 54.0% raisin tree seed extract, implement Root of kudzu vine hoveniae semoveniae semen mixed extract (general flavone content is 54.23%) prepared by example 1 compares sample, numbering be 1., 2., 3., ④；The composition of the preparation of embodiment 5 is chosen as test specimen, 5. numbering is；Choose weight and compare the root of kudzu vine：Hoveniae semoveniae semen：Royal jelly Extract=2:2:3 composition (the remarks prepared by embodiment 5：Each component of composition and present composition phase Same but proportion of composing is outside present composition proportion of composing) used as reference sample, 6. numbering is.Carry out ethanol simultaneously to small The influence of concentration of alcohol change in influence, the rat blood of mouse Sleep latency and the length of one's sleep, mouse liver protective effect examination Test, it is as a result as follows：
1. the influence experiment of Mice Hepatocytes Injured by Ethanol Sleep latency and the length of one's sleep
Dosage：5. 60kg adult is defined as 2.4g/ days composition of the present invention to sample dosage, and body is converted accordingly Weight is that 20g mouse are 252mg/kg to sample dosage.According to etc. dosage determine control sample than principle to sample 6., 4. mouse be to sample Dosage is 252mg/kg.The dosage such as foundation are to sample than principle, the root of kudzu vine, hoveniae semoveniae semen with reference to national regulation, royal jelly extract Day highest recommended dose determines that 1. control sample is 62mg/kg to sample amount；2. control sample is 252mg/kg to sample amount；Control sample 3. product are 252mg/kg to sample amount.
Testing program and data：Choose healthy Male Kunming strain mice 70,20 ± 2g of body weight.It is normal feed one week after with Machine is divided into 7 groups, and numbering is 1.~6. group and blank group.Wherein, 1.~6. group give the sample of reference numeral, blank group is not appointed Manage where.After 30min 1.~6. group and blank group give 60% ethanol (dosage is 0.32ml/20g).Record each group in detail is small Mouse righting reflex loss and recovery time, Sleep latency and the length of one's sleep are calculated, the results are shown in Table 5.
The Mice Hepatocytes Injured by Ethanol length of one's sleep of table 5 and Sleep latency influence result of the test
Group Number of animals Sleep latency (min) The length of one's sleep (min)
① 10 11.3±2.3 439±17.23
② 10 12.6±1.9 423±18.32
③ 10 11.9±2.4 428±17.96
④ 10 13.2±2.5 418±18.41
⑤ 10 17.5±2.8 340±17.89
⑥ 10 13.5±2.7 416±17.86
Blank group 10 9.8±0.1 480±0.10
Table 5 is found out：At the end of experiment, 1.~6. group compared to blank group Sleep latency, the length of one's sleep by different journeys Degree improves, and 5. group compared to 1., 2., 3., 4., 6. group and blank group Sleep latency, length of one's sleep p ＜ 0.01, with height Degree significant difference.
Conclusion (of pressure testing)：It is royal jelly extract, kudzu root extract, raisin tree seed extract, root of kudzu vine hoveniae semoveniae semen mixed extract, non- Ratio of the present invention prepares composition has certain improvement ethanol induced mice Sleep latency and with dormancy time function, but difference is not Significantly.5. composition of the present invention significantly extends ethanol induced mice Sleep latency, the pharmacological activity for curtailing sleep.
2. on concentration of alcohol change influence experiment in rat blood
Test article：1.~6. number sample, white wine (52 ° of Red Star strong, colourless liquor distilled from sorghum white wine of Beijing Red Star winery manufacture), body Weigh 200 ± 20g health male rats.
Give sample dosage：5. 60kg adult is defined as 2.4g/ days composition of the present invention to sample dosage, and body is converted accordingly Weight is that 200g rats are 216mg/kg to sample dosage.According to etc. dosage to sample than principle determine control sample 6., 4. give sample agent It is 216mg/kg to measure.The dosage such as foundation are to sample than principle, the root of kudzu vine, hoveniae semoveniae semen, royal jelly extract day with reference to national regulation Highest recommended dose determines that 1. control sample is 54mg/kg to sample amount；2. control sample is 216mg/kg to sample amount；Control sample 3. it is 216mg/kg to sample amount.
Testing program 1：Concentration of alcohol activity confirms in composition reduction rat blood of the present invention
Choose healthy male rat 30, it is normal feed one week after wine group after point blank control group, first medicine, first after drinking at random Medicine group, every group each 10.Fasting 12h before experiment, to sample, (blank control group gives white wine to gavage, first medicine group is filled after drinking on request Wine is given after wine group administration 30min after administration, first medicine after 30min after drinking, three groups are 0.015ml/g body weight to vina amount).Respectively 1h, 2h, 3h, 4h take blood from orbital venous plexus after before to sample and to sample, determine concentration of alcohol (remarks：Concentration of alcohol is using beautiful The dry chemical automatic clinical chemistry analyzers of Kodak of state VTTROS 250 are determined), the results are shown in Table 6, table 7.
Each time period concentration of alcohol (unit mol/L) of each group of table 6
7 liang of test groups concentration of alcohol (unit mol/L) of table
Time (h) Wine group after first medicine First medicine group after drinking P
Before sample 2.0±0 2.0±0
1 25.7±15.4 47.5±17.0 ＜ 0.01
2 43.4±15.2 41.3±15.9
3 42.0±16.0 44.4±16.2
4 31.0±16.5 37.6±16.9 ＜ 0.05
Conclusion (of pressure testing)：For compared to blank control group, either wine or first medicine, of the present invention group after drinking after first medicine Compound is respectively provided with significantly reduces concentration of alcohol effect in rat blood.And concentration of alcohol is lived during wine is to reducing rat blood after first medicine Property is stronger.
Testing program 2：Concentration of alcohol expression activitiy in present composition reduction rat blood
Choose healthy male rat 70, it is normal feed one week after be randomly divided into 7 groups, numbering is 1.~6. group and blank Group, every group each 10.Fasting 12h, 1.~6. organize gavage respectively and give corresponding test specimen, 1.~6. group and sky after 30min White group gives white wine by 0.015ml/g body weight gavages.Terminate rear 1h, 2h, 3h, 4h before to sample, to sample simultaneously from eye socket Veniplex takes blood, determines concentration of alcohol, the results are shown in Table 8.
Table 8 each group different time sections concentration of alcohol (unit mol/L) measurement result
Conclusion (of pressure testing)：For blank group, sample 1.~6. group make by certain rat blood concentration of alcohol that reduces With, but 1., 2., 3., 4., 6. sample difference is not obvious.And sample 5. (composition of the present invention) compared to 1., 2., 3., 4., 6. for there is pole significant difference (P ＜ 0.01), showing that 5. sample has significantly reduces concentration of alcohol in rat blood and makees With.
3. the protective effect of pair alcoholic liver injury
Laboratory sample and equipment：Sample 1.~6., electronic balance, UV752N ultraviolet-uisible spectrophotometers, Alpha-D2 Freeze dryer.
Dosage：Tested with reference to Mice Hepatocytes Injured by Ethanol Sleep latency and the influence of the length of one's sleep.
Testing program：Healthy Kunming kind female mice 80, body weight (20 ± 2) g, under same temperature, humidity freely Feed, water inlet feed one week after, be randomly divided into blank, model comparison, 1.~6. test group amount to 8 groups.Wherein, blank pair According to group and model group it is daily give 0.9% physiological saline feed, 1.~6. organize daily gavage and give counter sample, continuously feed four In week, Mouse Weight is weighed per weekend.In after surrounding last dose, disposably giving model group and 1.~6. organize 50% ethanol 12ml/kg BW, blank control group gives 0.9% physiological saline, and fasting can't help water 16h by sacrifice, and solution digs out whole livers It is dirty, pre- cold saline clean to washing lotion without color after, blot liver surface moisture.Clip 0.1g liver organizations are placed in small beaker In, prepare 9 times of physiological saline of liver weight, the physiological saline of total amount 2/3 is shifted in beaker, liver is shredded as early as possible, Enter glass homogenizer, remaining 1/3 rinse after pour into be homogenized together, be made 10% tissue homogenate, in centrifuge with 2000r/min is centrifuged 15min, takes supernatant detection GSH, MDA and TG, the index such as ADH, measurement result be shown in Table 9, table 10, table 11, Table 12, table 13.
The each group Mouse Weight testing result of table 9
Remarks：During off-test, each group changes of weight no significant difference (P ＞ 0.05).Show that each group mouse growth is normal, Without because of adverse events and adverse reaction caused by gavage sample.
The each group murine liver tissue GSH assay results of table 10
Group GSH(mg GSH/mg)
Blank group 26.4±1.1
Model group 16.2±0.6
① 17.5±0.8
② 17.8±0.7
③ 17.6±0.9
④ 19.0±0.9
⑤ 24.5±0.8
⑥ 19.1±1.1
Table 10 is found out：Blank group significantly reduces (P ＜ 0.01) compared to model group GSH contents, illustrates alcoholic liver injury Model modeling success.5. compared to 1., 2., 3., 4., 6. group and model control group compare, GSH changes of contents has aobvious test group Sex differernce (P ＜ 0.01) is write, is positive findings.
MDA assay results in each group murine liver tissue of table 11
Group MDA(nmol/mg)
Blank group 5.3±0.5
Model group 11.5±0.6
① 10.8±0.5
② 10.5±0.7
③ 10.7±0.6
④ 9.5±0.6
⑤ 7.4±0.5
⑥ 9.6±0.4
Table 11 is found out：Blank group significantly reduces (P ＜ 0.01) compared to model group GSH contents, illustrates alcoholic liver injury Model modeling success.5. compared to 1., 2., 3., 4., 6. group and model control group compare, MDA changes of contents has aobvious test group Sex differernce (P ＜ 0.01) is write, illustrates that composition described in embodiment 5 can reduce lipid peroxidation in the liver that ethanol causes, Play liver-protective effect.
TG content detection results in each group murine liver tissue of table 12
Group TG(mmol/L)
Blank group 0.6±0.03
Model group 1.3±0.12
① 1.3±0.02
② 1.2±0.03
③ 1.3±0.02
④ 1.1±0.03
⑤ 0.8±0.01
⑥ 1.1±0.02
Table 12 is found out：5. compared to 1., 2., 3., 4., 6. group and model control group compare, TG changes of contents has test group Have significant difference (P ＜ 0.01), illustrate the composition prepared by embodiment 5 can reduce drunk mouse liver tissue in TG contain Amount, mitigates liver fat and piles up, and plays liver-protective effect.
ADH determination of activity results in each group murine liver tissue of table 13
Group ADH(U/mg)
Blank group 1.1±0.11
Model group 0.9±0.12
① 1.0±0.20
② 1.1±0.30
③ 1.0±0.40
④ 1.2±0.20
⑤ 1.6±0.04
⑥ 1.2±0.03
Table 13 is found out：1.~6. group all show necessarily to improve ADH activity in mouse liver tissue relative to model group, 5. ADH activity improves nearly 60% and 77.8% compared with blank group and model group in group, illustrates the composition of gained in the embodiment of the present invention 5 The activity of ADH in mouse liver can not only be kept, moreover it is possible to excite the activity of ADH, accelerating alcohol metabolism in vivo reduces ethanol Negative effect to body.
In sum, the relieving alcoholism and protecting liver composition that significantly improves of the present invention has decomposing alcohol, prevention human body alcohol The effect be poisoned, sobered up.Secondly liver protection function can also effectively be played.
Above content is only specific embodiment of the invention further description made for the present invention, it is impossible to assert Specific implementation of the invention is confined to these modes.For general technical staff of the technical field of the invention, On the premise of not departing from present inventive concept, principle, some simple deductions or improvement can also be made, should all be considered as belonging to this hair Bright protection domain.

Claims (11)

1. it is a kind of to significantly improve the active composition of relieving alcoholism and protecting liver.It is characterized in that：The composition is by parts by weight 10~19 The royal jelly extract of part, the root of kudzu vine of 291~763 parts of parts by weight, the hoveniae semoveniae semen composition of 171~763 parts of parts by weight.
2. composition according to claim 1, it is characterised in that：The composition by 12~18 parts of parts by weight royal jelly Extract, the root of kudzu vine of 350~650 parts of parts by weight, the hoveniae semoveniae semen composition of 200~500 parts of parts by weight.
3. composition according to claim 1, it is characterised in that：The composition by 13~17 parts of parts by weight royal jelly Extract, the root of kudzu vine of 400~600 parts of parts by weight, the hoveniae semoveniae semen composition of 250~450 parts of parts by weight.
4. composition according to claim 1, it is characterised in that：The composition by 14~16 parts of parts by weight royal jelly Extract, the root of kudzu vine of 450~550 parts of parts by weight, the hoveniae semoveniae semen composition of 300~400 parts of parts by weight.
5. the composition according to claim 1-4, it is characterised in that：Royal jelly acid content is 10 in the royal jelly extract Between~50%, preferably 20%~40%, more preferably 30%~40%.
6. the composition according to claim 1-4, it is characterised in that：The root of kudzu vine, hoveniae semoveniae semen use 80% alcohol heat reflux Mixing is extracted.Wherein, solid-liquid ratio 1 is extracted:10th, 75~80 DEG C of temperature control, extraction time 1.5h, continuous extraction 3 times, extract solution decompression Concentrate drying obtains mixed extract.General flavone content >=30%.
7. the composition according to claim 5-6, it is characterised in that：The composition is to be carried royal jelly using organic solvent Thing and root of kudzu vine hoveniae semoveniae semen mixed extract mixed dissolution are taken, with Macrogol 4000, Macrogol 6000, PVP K30, pool Lip river One or more in Sha Mu -188, xylitol, sorbierite, citric acid as carrier, melt or using organic solvent dissolving after Add in royal jelly extract and root of kudzu vine hoveniae semoveniae semen mixed extract, be sufficiently mixed uniformly, boil off solvent and obtain extract in carrier In the co-precipitation solid dispersions that mix, through being drying to obtain composition of the present invention.
8. composition according to claim 7, it is characterised in that：Mix extraction with root of kudzu vine hoveniae semoveniae semen for royal jelly extract The organic solvent of thing dissolving is one or more in absolute ethyl alcohol, ethanol, acetone, chloroform, and solid-liquid ratio is 1:1~10, preferably It is 2~8, more preferably 3~6, most preferably 4~5.
9. composition according to claim 7, it is characterised in that：Carrier is using melting or organic solvent dissolution mechanism.Using When organic solvent dissolves, the organic solvent for being used is one or more in absolute ethyl alcohol, chloroform, acetone, ethanol, solid-liquid ratio It is 1:1~10, preferably 2~8, more preferably 3~6, most preferably 4~5.
10. composition according to claims 1 to 9, it is characterised in that：The composition have relieving alcoholism and protecting livers liver-protecting function/ Effect, in can be applied to medicine, health food, feature ordinary food, special medicine food, special diet food.
11. compositions according to claims 1 to 10, it is characterised in that：The composition can be added as needed on or not add Dosing product, health food, feature ordinary food, special medicine food, the acceptable auxiliary material of special diet food, further make Into solid orally ingestibles such as granule, tablet, hard capsule, dripping pills.
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115736063A (en) * 2022-11-02 2023-03-07 安徽中医药大学 Compound tea drink with effects of dispelling effects of alcohol and protecting liver and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104126796A (en) * 2014-07-18 2014-11-05 吴自伟 Health care food capable of dispelling effects of alcohol
CN106376927A (en) * 2016-08-25 2017-02-08 苏州神元生物科技股份有限公司 Health care product with auxiliary protective effect on chemical liver injury

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104126796A (en) * 2014-07-18 2014-11-05 吴自伟 Health care food capable of dispelling effects of alcohol
CN106376927A (en) * 2016-08-25 2017-02-08 苏州神元生物科技股份有限公司 Health care product with auxiliary protective effect on chemical liver injury

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
茹清静: "蜂王浆活性成分与护肝机制研究", 《中西医结合肝病杂志》 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115736063A (en) * 2022-11-02 2023-03-07 安徽中医药大学 Compound tea drink with effects of dispelling effects of alcohol and protecting liver and preparation method thereof

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