CN106822228A - A kind of subprostrate sophora polysaccharide active component and preparation method thereof - Google Patents
A kind of subprostrate sophora polysaccharide active component and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/489—Sophora, e.g. necklacepod or mamani
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- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of subprostrate sophora polysaccharide active component and preparation method thereof, described subprostrate sophora polysaccharide active component is that the Aqueous extracts of subprostrate sophora are carried out to be classified after alcohol precipitation to be dried to obtain, wherein mass percent >=65% containing total starches, the mass percent ＜ 0.5% containing matrine.Experiment shows：The acute toxicity and hepatotoxicity of the subprostrate sophora polysaccharide active component that the present invention is provided there occurs and significantly reduce, and with significant immunological enhancement, have the subprostrate sophora pharmaceutical preparation of clinical value significant to research and development；Also, the preparation method is simple, and reproducible, yield is high, is suitable to large-scale production, with industrial application value.
Description
Technical field
The present invention relates to a kind of subprostrate sophora polysaccharide active component and preparation method thereof, belong to pharmaceutical technology field.
Background technology
Subprostrate sophora is the dry root and rhizome of legume sophora tonkinensis Gapnep (Sophora tonkinensis Gagnep), is mainly originated in wide
West, Guangdong, Guizhou etc. save, taste bitter and cold, return lung warp.Pharmacopeia records subprostrate sophora has clearing heat and detoxicating, throat, dissipates swollen
Effect of pain.The main component of subprostrate sophora is：Alkaloids, flavonoids, organic acid, saponin(e and polysaccharide compound.Medicine
Reason experiment show, subprostrate sophora have antitumor, antiviral, anti-hepatitis, anti-arrhythmia etc. many-side pharmacological activity, and according to
Research report alkaloid component contained therein is generally the material base of these pharmacological activity.Modern study shows subprostrate sophora to liver
Significantly, such as the preparation Sophora Tankinensis containing total alkaloids of subprostrate sophora root shows scorching therapeutic effect through long-term clinical application, can drop
Low transaminase, improves immunity of organisms, has good therapeutic effect to CAH.Such as Chinese patent CN85100392A
A kind of method that treatment medicine for curing hepatitis is extracted in disclosing subprostrate sophora from plant, Chinese patent CN1457778A discloses mountain beans
The oral formulations of root, preparation method and application.The involved main secondary metabolism being in subprostrate sophora is produced in these patents
The composition of alkaloids of thing one.
But animal experiment study shows：Subprostrate sophora has hepatotoxicity, give rat 16g/kg Radix Sophorae Tonkinensis Boiled liquids can cause it is bright
Aobvious hepatic injury, and its damage mechanisms is relevant with the effect of inflammatory factor and lipid peroxidation, the hepatotoxicity wind agitation with carbon tetrachloride has
There is certain similitude (Chinese experimental pharmacology of traditional Chinese medical formulae magazine, the 18th phase of volume 19, in September, 2013, the 293-296 pages).
And preclinical study finds：Subprostrate sophora be not only dissolved in water but also be dissolved in the total extract of ethanol (based on alkaloid and flavonoids) to
, there is respiration inhibition, ultra-large type and the symptom such as trembles, twitches in mouse stomach (25g/kg), and even results in animal dead.Therefore,
Start to study the activity of subprostrate sophora non-alkaloid components in the recent period, for example：Chinese patent CN1306854A proposes one
The water extract-alcohol precipitation position of subprostrate sophora is planted, specific preparation method is：Subprostrate sophora is taken, heating extraction after being soaked in water, then through 0~3
After secondary hot water is extracted, filtering merges Aqueous extracts, concentration, plus ethanol, takes alcohol precipitation part and obtains final product." patent points out subprostrate sophora
In water extracting alcohol hypostasis be based on the macromolecular substances such as polysaccharide be non-alkaloid part；In addition, the experiment of the patent shows：
Water extracting alcohol hypostasis in subprostrate sophora can be reduced to CCL4Caused acute liver damage, can be such that rats death rate is dropped to by 30%
10%, with protect liver and immunoregulation effect.But so far there are no acute toxicity and liver on subprostrate sophora polysaccharide active component
The research report of the medical value at the position after the research report of toxicity and toxicity reduction.
The content of the invention
In view of the above-mentioned problems existing in the prior art, it is an object of the invention to provide a kind of acute toxicity and hepatotoxicity it is very low,
And the significant subprostrate sophora polysaccharide active component of immune-enhancing activity and preparation method thereof, to widen the clinical practice of subprostrate sophora.
Subprostrate sophora polysaccharide active component of the present invention, is that the Aqueous extracts of subprostrate sophora are carried out to be classified after alcohol precipitation to be dried to obtain,
Wherein mass percent >=65% containing total starches, the mass percent ＜ 0.5% containing matrine.
Preferably, mass percent >=70% containing total starches in the subprostrate sophora polysaccharide active component.
As further preferred scheme, mass percent >=85% containing total starches in the subprostrate sophora polysaccharide active component.
The preparation method of subprostrate sophora polysaccharide active component of the present invention, comprises the following specific steps that：
A) subprostrate sophora medicinal material is taken, is boiled with decocting, prepare Radix Sophorae Tonkinensis Boiled liquid；
B) at 75~80 DEG C, to Radix Sophorae Tonkinensis Boiled liquid be concentrated under reduced pressure into concentration for 1~2kg/L, then at 95~100 DEG C
Under carry out normal heating 1~2 hour, be cooled to room temperature, subprostrate sophora Aqueous extracts are obtained；
C) it is stirring while adding to the ethanol that volume fraction is 90~100% is added in step b) gained subprostrate sophora Aqueous extracts, extremely
The volume fraction of final ethanol is 75~85%, is stood, and collects sediment；
D) pressure reduction with classification drying is carried out to gained sediment：First drying under reduced pressure 10~14 hours, Ran Hou at 80 ± 2 DEG C
Drying under reduced pressure obtains final product the subprostrate sophora polysaccharide active component to absolutely dry at 75 ± 2 DEG C.
Preferably, the preparation of the Radix Sophorae Tonkinensis Boiled liquid, including following operation：
By subprostrate sophora medicinal material, 95~100 DEG C are heated to after soaking, carry out 1~3 time decoction, each decocting time be 0.5~
1.5 hours.
Preferably, decoct every time, the weight for adding water is 4~8 times of subprostrate sophora medicinal material weight.
Compared with prior art, the present invention has following conspicuousness beneficial effect：
1st, analyze after tested：Mass percent >=65% containing total starches in the subprostrate sophora polysaccharide active component that the present invention is provided,
The mass percent ＜ 0.5% of matrine；Relative to existing water extract-alcohol precipitation position, the subprostrate sophora polysaccharide that the present invention is provided is effective
The acute toxicity and hepatotoxicity at position there occurs and significantly reduce, and illustrate the present invention by controlling the content of matrine so that
The security of subprostrate sophora polysaccharide active component has obtained conspicuousness raising；
2nd, show through in vitro and in vivo experiment：There is the subprostrate sophora polysaccharide active component that the present invention is provided significant Immune-enhancing effect to make
With, illustrate the present invention by rationally control subprostrate sophora polysaccharide active component in total starches and matrine content, not only substantially
Reduce toxicity, and there is significant immune-enhancing activity simultaneously, relative to prior art, achieve conspicuousness progress and
Unexpected effect, it is significant to subprostrate sophora pharmaceutical preparation of the research and development with clinical value；
3rd, in addition, preparation method of the invention is simple, reproducible (active component finger-print is similar obtained in different batches
99%) degree is up to, and yield may be up to 9.11%, be suitable to large-scale production, with industrial application value.
Brief description of the drawings
Fig. 1 is the finger-print of the different batches sample prepared by embodiment 2.
Specific embodiment
With reference to specific embodiment and comparative example, the present invention is expanded on further.It should be understood that these embodiments are merely to illustrate
The present invention rather than limitation the scope of the present invention.The experimental technique of unreceipted actual conditions in the following example, generally according to
Normal condition or according to the condition proposed by manufacturer.
Embodiment 1：The preparation of subprostrate sophora polysaccharide active component
A) subprostrate sophora medicine materical crude slice 8kg is taken, 60min is soaked with 48kg water, be then heated to reflux decocting 1h, the mesh of decoction liquor 200
Filtering, the dregs of a decoction add 48kg water and are heated to reflux decocting 1h, and the filtering of the mesh of decoction liquor 200 merges filtrate twice, obtains mountain
Beans root decocting liquid；
B) at 75~80 DEG C, to Radix Sophorae Tonkinensis Boiled liquid be concentrated under reduced pressure into concentration for 1.5kg/L, then enter at 98 DEG C
Row normal heating 1.5 hours, is cooled to room temperature, and subprostrate sophora Aqueous extracts are obtained；
C) it is stirring while adding to the ethanol that volume fraction is 95% is added in step b) gained subprostrate sophora Aqueous extracts, to final second
The volume fraction of alcohol is 80%, is stood, and collects sediment；
D) pressure reduction with classification drying is carried out to gained sediment：First drying under reduced pressure 12 hours at 80 DEG C, then subtract at 75 DEG C
Press dry dry to absolutely dry, obtain final product the subprostrate sophora polysaccharide active component 728.8g, yield is 9.11%.
Using phenol-sulfuric acid process, with spectrophotometry, with glucose meter, contain in the subprostrate sophora polysaccharide active component
Sugar amount is 88wt%.
Analyzed through HPLC：The content containing matrine (MT) is 0.46wt% in the subprostrate sophora polysaccharide active component, oxygen-containing
The content for changing matrine (OMT) is 0.046wt%.
Embodiment 2：The study on the stability of preparation technology
According to the preparation technology of embodiment 1, three batch subprostrate sophora polysaccharide active components are prepared in repetition, in being made by HPLC
Similarity between medicine finger-print, contrast different batches sample.HPLC conditions are as follows：
As shown in Figure 1, the R in figure is the sample of embodiment 1 to experimental result, and S1, S2, S3 are respectively manufactured in the present embodiment batch
Secondary 1-3 samples, as seen from Figure 1：The fingerprint similarity of sample R, S1, S2, S3 reaches 99%, illustrates system of the invention
Standby process stabilizing, it is reproducible, it is expected to carry out industrialized production.
Comparative example：The preparation at subprostrate sophora water extract-alcohol precipitation position
With reference to Chinese patent CN1306854A embodiments 1：
The subprostrate sophora 8kg for shredding is weighed, with 12 multiple dose water soaked overnights, heating extraction 2h pours out aqueous, is centrifuged while hot
(3000r/min, l0min), the dregs of a decoction continue plus 10 times, 8 multiple dose water difference heating extraction 1.5h, 1h, each pour out aqueous
It is centrifuged while hot, aqueous antigradient is concentrated into 1：1 (1g crude drugs are equivalent to 1mL), adds the ethanol that volume fraction is 95%
Alcohol content up to 80%, stand at low temperature is set overnight to be centrifuged afterwards, precipitation Jia 1：It is centrifuged again after 50 hot water dissolvings, water intaking liquid adds body
Fraction is that 95% ethanol makes alcohol amount up to 80%, stand at low temperature overnight be centrifuged afterwards, and precipitation plus the stirring of 95% ethanol are centrifuged again,
Precipitation vacuum drying (40 DEG C), obtains subprostrate sophora water extract-alcohol precipitation position 257.6g, and yield is 3.20%.
Using phenol-sulfuric acid process, with spectrophotometry, with glucose meter, contain in the subprostrate sophora water extract-alcohol precipitation position
Sugar amount is 58wt%.
Analyzed through HPLC：The content containing matrine (MT) is 0.65wt% in the subprostrate sophora water extract-alcohol precipitation position, oxygen-containing
The content for changing matrine (OMT) is 0.132wt%.
First, toxotest experiment
Subprostrate sophora water extract-alcohol precipitation position prepared by subprostrate sophora polysaccharide active component and comparative example prepared by embodiment 1, acts on respectively
In the wild type AB systems zebra fish for the treatment of to 5dpf (after fertilization 5 days), its acute toxicity and hepatotoxicity are evaluated.
With the wild type AB systems zebra fish juvenile fish of drug-treated certain phase to be measured to 5dpf, series concentration is set, while setting
Blank control group, each concentration processes 30 tail zebra fish.During drug-treated, the zebra fish that each experimental group is counted daily is dead
Quantity of dying simultaneously is removed in time, and concentration-destruction curve is fitted by curve matching by the softwares of OriginPro 8.0, can be in the hope of LC50、
LC10, test result is shown in Table shown in 1~3.
The zebra fish death rate (n=30) that the subprostrate sophora polysaccharide active component of table 1 induces
| Concentration (μ g/mL) | With fish number (tail) | Death toll (tail) | The death rate (%) |
| 2500 | 30 | 0 | 0 |
| 3000 | 30 | 0 | 0 |
| 3500 | 30 | 1 | 3.3 |
| 4000 | 30 | 2 | 6.7 |
| 4500 | 30 | 5 | 16.7 |
| 5000 | 30 | 30 | 100 |
The zebra fish death rate (n=30) that the subprostrate sophora water extract-alcohol precipitation position of table 2 induces
| Concentration (μ g/mL) | With fish number (tail) | Death toll (tail) | The death rate (%) |
| 600 | 30 | 0 | 0 |
| 800 | 30 | 1 | 3.3 |
| 1000 | 30 | 22 | 73.3 |
| 1200 | 30 | 30 | 100 |
| 1400 | 30 | 30 | 100 |
The toxicity of table 3 compares
It is visible to table 3 with reference to table 1：The concentration of the subprostrate sophora polysaccharide active component that the death rate of zebra fish 100% of induction needs
(5000 μ g/mL) will be significantly larger than the concentration (1200 μ g/mL) at subprostrate sophora water extract-alcohol precipitation position, in acute toxicity testing,
The LC of subprostrate sophora polysaccharide active component10And LC50Value is the LC at subprostrate sophora water extract-alcohol precipitation position10And LC505 times of value, and
In hepatotoxicity experiment, the LC of subprostrate sophora polysaccharide active component10Value is also the LC at subprostrate sophora water extract-alcohol precipitation position105 times of value
It is many；Illustrate, the content that the present invention passes through the matrine in rationally control subprostrate sophora polysaccharide active component, hence it is evident that reduce toxicity,
Relative to prior art, conspicuousness progress is achieved, had to subprostrate sophora pharmaceutical preparation of the research and development with clinical value
It is significant.
2nd, to the in vitro test of normal mouse lymphocyte immunoregulatory activity
The subprostrate sophora water extract-alcohol precipitation position prepared by the subprostrate sophora polysaccharide active component and comparative example of observing the preparation of embodiment 1, it is right
The influence of the mouse T lymphocyte expanding capacity of mitogen ConA inductions, studies its immunoregulation effect.
(1) prepared by SPL：The de- vertebra of BALB/C mice is put to death, aseptic to take its spleen, is made single cell suspension,
After removal red blood cell, cell concentration is adjusted with the RPMI-1640 nutrient solutions containing 10%FBS；
(2) lymphocyte proliferation assay：Lymphocyte suspension 4 × 10 is added in 96 porocyte culture plates5Individual/hole, 50 μ L
ConA (the μ g/mL of final concentration 5), takes subprostrate sophora polysaccharide active component and subprostrate sophora water extract-alcohol precipitation position, is configured to respectively dense eventually
Degree be respectively 100 μ g/mL, 50 μ g/mL, 25 μ g/mL to drug solns；Each concentration three wells, is 200 μ L per hole cumulative volume,
And set corresponding without ConA control wells and without drug control hole；37 degree, 5%CO2, cultivate 48 hours；Culture terminates preceding 8
Hour, 25 μ L are added per hole3H- thymidylic acids (10 μ Ci/mL)；Continue to cultivate to experiment and terminate；By cell cell
Collect instrument to collect to glass fibre membrane, add scintillation solution after Beta numerations instrument (MicroBeta Trilux, PerkinElmer)
Read and mix cell DNA3H-TdR is measured, and the situation of cell propagation is represented with cpm values, and result of the test is shown in Table 4.
Influence of the table 4 to ConA inducing mouse T Proliferation of lymphocytes is compared
In table, compare with control group,* *p<0.001。
From table 4：The proliferation function of the normal mouse T lymphocytes that subprostrate sophora polysaccharide active component group is induced ConA
There is conspicuousness compared with control group, and with concentration dependent；Illustrate, subprostrate sophora polysaccharide active component of the invention can promote ConA
The breeder reaction of the normal mouse T lymphocytes of induction, has significant humidification to the cellular immune function of mouse.
3rd, to the in vivo studies of hypoimmunity mice
The subprostrate sophora water extract-alcohol precipitation position prepared by the subprostrate sophora polysaccharide active component and comparative example of observing the preparation of embodiment 1, it is right
The regulatory function of the mouse model of immunocompromised caused by irradiated, studies its immunological enhancement in whole animal.
BALB/C mice body weight irradiates X-ray, irradiation condition 3GRADE. irradiation times three minutes in 18-22g；After irradiation
5 groups are randomly divided into according to body weight, respectively：Normal group, model group, low dose group, middle dose group and high dose group；Administration
Two weeks, the CMC-Na suspending extracts with 0.3% made its concentration respectively 25mg/kg, 50mg/kg and 100mg/kg.Experiment
Result is as follows：
(1) sign and body weight
After modeling 15 days, model mice shows obvious Body weight loss, and hair color is unglazed, and burnout moves few food less；And give
The above-mentioned symptom of each mouse of medicine group is eased and improves for model group.
After being administered 15 days, the body weight of model group mouse compared with control group, p<0.01, show that model group has significantly with normal group
Sex differernce, illustrates modeling success.But each administration group compares with model group, p>0.05, it is not statistically significant, but there is body weight
The trend of recovery, detailed results are shown in Table 5.
Table 5 influences on the body weight of immunosuppression mouse model
In table, compare with model group,*p<0.01。
From table 5：Subprostrate sophora polysaccharide active component group has restitution, and effect to the body weight of immunosuppression mouse model
Effect is slightly above subprostrate sophora water extract-alcohol precipitation position group.
(2) size and form of immune organ and thymus gland spleen index
Mouse support cervical vertebra is put to death, Spleen Size is observed and is weighed, detailed results are shown in Table 6.
Influence of the table 6 to hypoimmunity mice spleen weight
In table, compare with model group,*p<0.05,*p<0.01。
From table 6：Model group spleen is small compared with control group, and p<0.01, illustrate modeling success；But subprostrate sophora polysaccharide has
Effect position group and subprostrate sophora water extract-alcohol precipitation position group are on the rise compared with model group, especially subprostrate sophora polysaccharide active component
25mg/kg dosage groups compared with model group, p<0.05, with significant difference, illustrate subprostrate sophora polysaccharide active component
25mg/kg dosage groups have obvious immunological enhancement.
The thymus gland and spleen filter paper for winning mouse blot mass metering after residual blood, are respectively divided by mouse weight multiplied by with 10, obtain
To Thymus and Spleen index, detailed results are as shown in table 7.
Influence of the table 7 to hypoimmunity mice index and spleen index and thymus index
In table, compare with model group,*p<0.05。
From table 7：The thymus gland spleen index of model group is compared with control group, p<0.05, illustrate immunodeficiency models modeling success；But
Subprostrate sophora polysaccharide active component group and subprostrate sophora water extract-alcohol precipitation position group can stimulate the increasing of mouse spleen thymic factor D injection upon administration
Plus, and subprostrate sophora polysaccharide active component group thymus index increase becomes apparent, especially the effective portion of 25mg/kg subprostrate sophoras polysaccharide
The thymus index of hyte increases has significant difference, illustrates subprostrate sophora polysaccharide active component of the invention to hypoimmunity mice
With notable immunological enhancement, and immunological enhancement is apparently higher than subprostrate sophora water extract-alcohol precipitation position.
4th, ConA stimulates the proliferation test of Mouse spleen cells
In order to confirm the immunopotentiating ability of subprostrate sophora polysaccharide active component and subprostrate sophora water extract-alcohol precipitation position with the presence or absence of concentration according to
Lai Xing, further carrying out ConA stimulates the proliferation experiment of Mouse spleen cells：
After administration one hour, the de- cervical vertebra of the mouse of different groups is put to death；Take out spleen mill spleen, mouse spleen lymphocyte 4 × 105
Individual/hole is inoculated in 96 orifice plates, while adding ConA, makes its final concentration of 0.5 μ g/mL, each sample three wells, per hole
Cumulative volume is 200 μ L；5%CO248h is cultivated in incubator, culture terminates preceding 8h and mixes 0.25 μ Ci3H-thymidine, training
At the end of supporting, culture plate is frozen in -20 DEG C of refrigerators；The cell cell collector of freeze thawing is collected to glass fibre during measure
On film, add scintillation solution that the 3H-thymidine for mixing cell DNA is read after Beta calculating instruments, represented with cpm values thin
Born of the same parents' proliferative conditions；Detailed results are as shown in table 8.
The Cell proliferation in vivo experimental result of table 8
In table, compare with model group,*p<0.05,*p<0.01,* *p<0.001。
From table 8：Subprostrate sophora polysaccharide active component group and two groups of administration groups of subprostrate sophora water extract-alcohol precipitation position group and model group phase
Compare the multiplication capacity that can improve immunocyte in spleen, and with concentration-dependent relation.
It is visible to table 8 with reference to table 4：Subprostrate sophora polysaccharide active component of the invention has good immunological enhancement, and
Its activity is also advantageous over subprostrate sophora water extract-alcohol precipitation position.
To sum up test visible：Content by controlling total starches and matrine in subprostrate sophora polysaccharide active component of the invention, no
Toxicity is only significantly reduced, and has significant immune-enhancing activity, relative to prior art, achieve conspicuousness simultaneously
Progressive and unexpected effect, it is significant to subprostrate sophora pharmaceutical preparation of the research and development with clinical value；
Also, the preparation method is simple, yield is high, good stability, it is easy to accomplish large-scale production, to promoting the wide of subprostrate sophora
General application has.
Finally need it is pointed out here that be：The above is only part preferred embodiment of the invention, it is impossible to be interpreted as protecting the present invention
Protect the limitation of scope, some nonessential modifications and adaptations that those skilled in the art's the above of the invention is made
Belong to protection scope of the present invention.
Claims (6)
1. a kind of subprostrate sophora polysaccharide active component, it is characterised in that：It is to be classified drying after carrying out alcohol precipitation to the Aqueous extracts of subprostrate sophora
Obtain, wherein mass percent >=65% containing total starches, the mass percent ＜ 0.5% containing matrine.
2. subprostrate sophora polysaccharide active component according to claim 1, it is characterised in that：The effective portion of subprostrate sophora polysaccharide
Mass percent >=70% containing total starches in position.
3. subprostrate sophora polysaccharide active component according to claim 2, it is characterised in that：The subprostrate sophora polysaccharide active component
In mass percent >=85% containing total starches.
4. a kind of method for preparing the subprostrate sophora polysaccharide active component in claim 1-3 described in any one, it is characterised in that：
Comprise the following specific steps that：
A) subprostrate sophora medicinal material is taken, is boiled with decocting, prepare Radix Sophorae Tonkinensis Boiled liquid；
B) at 75~80 DEG C, to Radix Sophorae Tonkinensis Boiled liquid be concentrated under reduced pressure into concentration for 1~2kg/L, then at 95~100 DEG C
Under carry out normal heating 1~2 hour, be cooled to room temperature, subprostrate sophora Aqueous extracts are obtained；
C) it is stirring while adding to the ethanol that volume fraction is 90~100% is added in step b) gained subprostrate sophora Aqueous extracts, extremely
The volume fraction of final ethanol is 75~85%, is stood, and collects sediment；
D) pressure reduction with classification drying is carried out to gained sediment：First drying under reduced pressure 10~14 hours, Ran Hou at 80 ± 2 DEG C
Drying under reduced pressure obtains final product the subprostrate sophora polysaccharide active component to absolutely dry at 75 ± 2 DEG C.
5. method according to claim 4, it is characterised in that：The preparation of the Radix Sophorae Tonkinensis Boiled liquid, including following behaviour
Make：By subprostrate sophora medicinal material, 95~100 DEG C are heated to after soaking, carry out 1~3 time decoction, each decocting time be 0.5~
1.5 hours.
6. method according to claim 5, it is characterised in that：Decoct every time, the weight for adding water is subprostrate sophora medicinal material weight
4~8 times of amount.
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|---|---|---|---|---|
| CN1306854A (en) * | 2000-01-31 | 2001-08-08 | 陈晓萍 | Water extracted and alcohol precipitated matter of subprostrate sophora and its application in medicine |
| KR20020044746A (en) * | 2000-12-06 | 2002-06-19 | 신민규 | Extract of Sophorae Tonkinensis Radix for the prevention and treatment of osteoporosis |
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| CN1306854A (en) * | 2000-01-31 | 2001-08-08 | 陈晓萍 | Water extracted and alcohol precipitated matter of subprostrate sophora and its application in medicine |
| KR20020044746A (en) * | 2000-12-06 | 2002-06-19 | 신민규 | Extract of Sophorae Tonkinensis Radix for the prevention and treatment of osteoporosis |
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| 吕莉莉等: "基于功效和物质基础的山豆根毒性研究进展", 《中国药物警戒》 * |
| 董群等: "山豆根多糖的性质和化学组成", 《中国药学杂志》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575758A (en) * | 2021-08-04 | 2021-11-02 | 广西大学 | Composite probiotics and preparation method thereof |
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