CN106818884B - Production device for prothymus gland extract of the yellow antler to increase the yield of biocontrol bacteria against citrus diseases - Google Patents
Production device for prothymus gland extract of the yellow antler to increase the yield of biocontrol bacteria against citrus diseases Download PDFInfo
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- CN106818884B CN106818884B CN201710077055.6A CN201710077055A CN106818884B CN 106818884 B CN106818884 B CN 106818884B CN 201710077055 A CN201710077055 A CN 201710077055A CN 106818884 B CN106818884 B CN 106818884B
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- 239000000284 extract Substances 0.000 title claims abstract description 68
- 241000207199 Citrus Species 0.000 title claims abstract description 44
- 235000020971 citrus fruits Nutrition 0.000 title claims abstract description 44
- 201000010099 disease Diseases 0.000 title claims abstract description 44
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 44
- 230000000443 biocontrol Effects 0.000 title claims abstract description 40
- 210000004907 gland Anatomy 0.000 title claims abstract description 40
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 11
- 241000894006 Bacteria Species 0.000 title claims description 12
- 210000003056 antler Anatomy 0.000 title 1
- 239000007787 solid Substances 0.000 claims abstract description 16
- 238000005520 cutting process Methods 0.000 claims description 59
- 239000010935 stainless steel Substances 0.000 claims description 34
- 229910001220 stainless steel Inorganic materials 0.000 claims description 34
- 229910000838 Al alloy Inorganic materials 0.000 claims description 26
- 210000001015 abdomen Anatomy 0.000 claims description 26
- 239000004033 plastic Substances 0.000 claims description 26
- 229920003023 plastic Polymers 0.000 claims description 26
- 238000000605 extraction Methods 0.000 claims description 17
- 229910000831 Steel Inorganic materials 0.000 claims description 10
- 239000010959 steel Substances 0.000 claims description 10
- 210000000683 abdominal cavity Anatomy 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- 238000007599 discharging Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 7
- 238000011084 recovery Methods 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 230000006698 induction Effects 0.000 claims description 4
- 239000011521 glass Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims 9
- 239000000463 material Substances 0.000 claims 7
- 230000002093 peripheral effect Effects 0.000 claims 3
- 244000153234 Hibiscus abelmoschus Species 0.000 claims 2
- 210000002784 stomach Anatomy 0.000 claims 2
- 210000000115 thoracic cavity Anatomy 0.000 claims 2
- 241000051764 Lasius umbratus Species 0.000 claims 1
- 210000000038 chest Anatomy 0.000 claims 1
- 229920001296 polysiloxane Polymers 0.000 claims 1
- 210000001541 thymus gland Anatomy 0.000 claims 1
- 241001660113 Oecophylla smaragdina Species 0.000 abstract description 53
- 238000000034 method Methods 0.000 abstract description 31
- 230000008569 process Effects 0.000 abstract description 24
- 230000000694 effects Effects 0.000 abstract description 8
- 241000196324 Embryophyta Species 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 4
- 230000007170 pathology Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 34
- 238000004064 recycling Methods 0.000 description 12
- 240000005373 Panax quinquefolius Species 0.000 description 7
- 235000003140 Panax quinquefolius Nutrition 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 238000010586 diagram Methods 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 229930014550 juvenile hormone Natural products 0.000 description 4
- 239000002949 juvenile hormone Substances 0.000 description 4
- 150000003633 juvenile hormone derivatives Chemical class 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 238000005086 pumping Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 239000003899 bactericide agent Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 230000003628 erosive effect Effects 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 241001272720 Medialuna californiensis Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 239000013043 chemical agent Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000011031 large-scale manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 231100000397 ulcer Toxicity 0.000 description 2
- 210000001364 upper extremity Anatomy 0.000 description 2
- 241000186046 Actinomyces Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 241001672694 Citrus reticulata Species 0.000 description 1
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000589655 Xanthomonas citri Species 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 239000004411 aluminium Substances 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
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- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 238000005381 potential energy Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000003319 supportive effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 231100000816 toxic dose Toxicity 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/04—Solvent extraction of solutions which are liquid
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
Improve citrus disease biocontrol bacterial strain yield Oecophylla smaragdina prothoracic gland extract process units, belong to Plant Pathology technical field, it is characterized by: grooving wall (26) lattice-shaped of the grooving (10) links together, grooving chamber (25) is multiple, arranged in rows, all ant peduncle swaging clamps (20) of every row are located point-blank, exhaust tube (12) separates branch pipe in the hollow part of ant peduncle cutter solid plate (22), the corresponding ant peduncle cutter (27) of each branch pipe, branch pipe lumen is communicated with the space in ant peduncle cutter body (23), one piston is set in branch pipe lumen, ant peduncle cutter body (23) can stretch.Improve the production of citrus disease biocontrol bacterial strain yield Oecophylla smaragdina prothoracic gland extract process units simply, strong operability is low in cost, and effect is obvious.
Description
Technical field
The present invention relates to a kind of raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units, belong to
Plant Pathology technical field.
Background technique
Citrus (Citrus reticulata Blanco), belongs to Rutaceae Citrus, and property is liked warm and moist weather, is distributed in
It is the torrid zone, subtropical evergreen fruit trees between 16-37 ° of north latitude.The 4-5 month at its florescence, the fruiting period 10-12 month are the precious fruits of the mankind
Product.However, an important factor for disease is puzzlement Orange Producing all the time.Currently, in actual production, controlling citrus
The main means of disease are using chemical bactericide, and the long-term a large amount of drug resistances for causing germ to generate using chemical agent are not only
The protection effect of chemical agent is reduced, while also causing residual toxic dose of the pesticide on fruit and increasing, to threaten the mankind's
Health and ecological safety.Therefore, there is an urgent need to research and develop completely new, nontoxic, efficient, environmentally friendly biological prevention and control agent in production, to take
In generation, reduces use of the chemical bactericide in prevention and treatment citrus disease.Since microbial resources are abundant, breeding is rapid, antibacterial anti-corrosion
Effect is obvious, and nontoxic, at low cost, so being considered as one of the method for being most hopeful to substitute chemical bactericide.In recent years,
Many scholars will be also placed in using on beneficial microbe the prevention and treatment of citrus disease, it was found that some preferable biocontrol bacterial strains.Than
Microorganism separation such as is carried out by dilution method to the soil sample acquired from south jiangxi citrus orchard, passes through experiment screening in ware to actinomyces strain
ML27, the bacterial strain is to citrus anthracnose bacterium (Colletotrichum gloeosporioides) and ulcer bacteria
(Xanthomonas axonopodis pv.citri) has to be acted on compared with high inhibition, and effect stability, to the inhibiting rate of anthrax bacteria
It is 72.15%, the inhibition zone to ulcer bacteria is 2.05cm (Liu Bing etc., 2011).In addition, being stored after citrusfruit harvesting
20 multiple diseases can occur in transportational process, wherein the largest loss of rotting caused by infecting with mould, green mold and anthrax-bacilus.Mesh
Before, citrus disease, which shows preferable biocontrol microorganisms, in prevention and treatment storage transport the molten bacillus of producing enzyme, bacillus subtilis, saccharomycete etc..
These biocontrol microorganisms are of great significance to Orange Producing and sale, there is good research and development and application in terms of citrus disease prevention and treatment
Prospect.However in the actual production process, all kinds of biocontrol microorganisms are after cultivating to logarithmic growth phase, it is divided over time
Activity gradually decreases.Many times, it is more than sufficient only to supply experiment demand for the biocontrol microorganisms of culture, but such as large-scale production is wanted to use
It is far from enough in practicing, therefore how to develop a kind of method that can improves biocontrol microorganisms yield within the unit time and become urgency
A great problem to be solved.Oecophylla smaragdina prothoracic gland extract, which mainly contains, promotes the substances such as fissional juvenile hormone, if will
Its separating and extracting comes out, and is mixed into the culture solution of culture citrus disease Biocontrol Strain, then the production of citrus disease biocontrol microorganisms can be improved
Amount.However, the acquisition of Oecophylla smaragdina prothoracic gland extract is more many and diverse, whole process careless manipulation is easy to failure, therefore wants benefit
Yong Oecophylla smaragdina prothoracic gland extract improves the yield of citrus disease biocontrol microorganisms, and invention improves citrus disease biocontrol microorganisms yield Huang Suede
Ant prothoracic gland extract process units is necessary.
Summary of the invention
In order to overcome the problems, such as that biocontrol microorganisms yield is not easy to improve in the unit time in biocontrol microorganisms large-scale production, the present invention is mentioned
It has supplied to improve citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units, which utilizes Oecophylla smaragdina prothoracic gland
Extract, which mainly contains, promotes the substances such as fissional juvenile hormone, is isolated and extracts, is mixed into citrus disease biological and ecological methods to prevent plant disease, pests, and erosion
In the culture solution of bacteria strain culture, to achieve the purpose that improve citrus disease biocontrol microorganisms yield.
The technical solution adopted by the present invention to solve the technical problems is:
Raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units of the invention, by cutting ant device
43 and extraction equipment 46 form, it is characterised in that: the stainless steel of grooving (10) for cutting ant device 43, plastics matter, aluminium close
Golden or pottery, length are 8-40 centimetres, and it is highly 5-25 centimetres that width, which is 5-35 centimetres, including reclaiming plate (7), discharging
Plate shake hands (8), discharging board slot (9), negative pressure chamber (11), exhaust tube (12), aspiration pump plug (13), aspiration pump power supply line (14),
Aspiration pump (15), escape pipe (16), grooving chamber (25), grooving wall (26), ant peduncle cutter (27)；The reclaiming plate (7) is
Positioned at the plate of grooving (10) bottom center tool groove, stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 2-20
Millimeter, length are 8-40 centimetres, and width is 5-30 centimetres；The discharging board slot (9) is to cut ant device base portion (44) to hold out
There is the opening being adapted with reclaiming plate (7) cross section size in the space of flitch (7) on cutting ant device wall (1)；Described
Negative pressure chamber (11) is located at grooving (10) bottommost, and the above is reclaiming plate (7), and side is connected to exhaust tube (12)；The pumping
Pipe (12) is rubber mass hose, and lumen diameter is 3-10 millimeters, and 1-5 millimeters of wall thickness, tube wall is interior to be supported by coil spring；It is described
Escape pipe (16) be rubber mass hose, lumen diameter is 5-20 millimeter, and 1-5 millimeters of wall thickness, tube wall is interior by coil spring branch
Support；The stainless steel of grooving wall (26), plastics matter, aluminium alloy matter or pottery, with a thickness of 1-10 millimeters, lattice-shaped is connected to one
It rises, the space surrounded is grooving chamber (25)；The grooving chamber (25) is multiple, arranged in rows, two adjacent cutback chambers (25)
Between separated by grooving wall (26), the length of each grooving chamber (25) is 6-8 millimeter, and it is highly 4-20 lis that width, which is 3-5 millimeters,
Rice, it is long equal to one ant peduncle swaging clamp (20) of setting, all ant peduncle swaging clamps (20) of every row at 2/3 in each grooving chamber (25)
Point-blank, grooving chamber (25) is divided by ant peduncle swaging clamp (20) contains ant abdominal cavity (18) and Sheng (21) two, ant cephalothorax chamber at place
Point, it is connected between two parts by ant peduncle swaging clamp seam (19)；The ant peduncle swaging clamp (20) includes left and right two parts, two parts
Between gap be width be 0.5-1 millimeter ant peduncle swaging clamp stitch (19) , Oecophylla smaragdina abdomens are shorter, stay in and account for grooving chamber (25)
In the Sheng ant abdominal cavity (18) of volume 1/3, head and chest are longer, stay in the Sheng ant cephalothorax chamber for accounting for grooving chamber (25) volume 2/3
(21) in；Ant peduncle swaging clamp (20) free edge ant peduncle swaging clamp seam (19) to the direction of grooving wall (26) have one tiltedly
Face；The ant peduncle cutter (27) is multiple, corresponding with grooving chamber (25), i.e., each ant peduncle cutter (27) is one corresponding
Grooving chamber (25), including ant peduncle cutter solid plate (22), ant peduncle cutter body (23) and ant peduncle cutoff edge (24)；The ant
Peduncle cutter solid plate (22) is the plate of cuboid, and ant peduncle cutter solid plate (22) side edge center is connected to exhaust tube
(12), ant peduncle cutter solid plate (22) is intermediate empty, the exhaust tube (12) part hollow in ant peduncle cutter solid plate (22)
Separate branch pipe, the corresponding ant peduncle cutter (27) of each branch pipe, branch pipe lumen and the space phase in ant peduncle cutter body (23)
It is logical, a piston is set in branch pipe lumen, and piston passes through a steel wire and ant in the flanking central of ant peduncle cutter body (23)
Peduncle cutoff edge (24) base portion links together, and is connected to one between ant peduncle cutoff edge (24) and ant peduncle cutter solid plate (22)
Crust is made by silica gel in a spring, the ant peduncle cutter body (23), and there are spring and steel wire in inside, can stretch.
It is described cut ant device (43) include cut ant device wall (1), stock guide (2), feed inlet (3), charging lid (4),
Stock guide mouth (5), striker plate (6), grooving (10)；The ant device wall (1) of cutting is the outer wall for cutting ant device (43), stainless steel
Matter, plastics matter, aluminium alloy matter or pottery, with a thickness of 1-10 millimeters；The ant device chamber (17) of cutting is by cutting ant device wall
(1) cavity surrounded, including cutting (45) at the top of ant device and cutting the internal space of ant device base portion (44)；The stock guide
(2) it is located at and cuts intermediate at the top of ant device (45), stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 0.5-5 millimeters,
Width is 1-3 centimetres, and the opening that is surrounded of centre is known as stock guide mouth (5), the plane where stock guide mouth (5) and cuts ant device
Top (45) center and the intersection point for cutting line segment between ant device base portion (44) upper face center are the midpoint of the line segment, stock guide
(2) angle between the plane where stock guide mouth (5) is 30 ° -45 °, and stock guide (2) neighboring, which is fixed on, cuts ant device
Top (45) inner surface；The feed inlet (3) cuts ant device top (45) and cuts ant device wall close to center 2-5 centimeters
(1) oblong openings on, length are 2-10 centimetres, and width is 1.5-8 centimetres；The stainless steel of charging lid (4), modeling
Expect matter, aluminium alloy matter or pottery, with a thickness of 0.5-5 millimeters, length is 2.2--10.2 centimetres, and width is 1.52-8.2 centimetres,
Top edge is fixed on the outer surface that feed inlet (3) upper limb cuts ant device wall (1) by hinge, and lower edge and both side edges are free；It is described
Striker plate (6) be grooving (10) upper surface periphery and the template knot for cutting connection between the periphery of ant device base portion (44) upper surface
Structure, stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 0.5-5 millimeters, with putting down where grooving (10) upper surface
Angle between face is 30 ° -45 °.
The extraction equipment (46) includes extractor (28), electromagnetic oven (29), substrate (30), extract liquor recycling bin
(31), extract liquor recycling mouth (32), grinder are consolidated the wing (33), grinder motor (34), grinder motor power-supply wire (35), are crushed
Device cylinder (36), grinder chamber (37), grinder knife handle (38), grinder supporting network (39), grinder knife (40), electromagnetic oven power supply
Line (41), extractor chamber (42)；The stainless steel of substrate (30), plastics matter, aluminium alloy matter or the pottery, with a thickness of 1-10
Centimetre；Described extractor (28) nature of glass, base portion is substantially spherical in shape, and diameter is 10-30 centimetres；Neck is cylindrical, and diameter is
5-10 centimetres, be highly 5-10 centimetres；The internal space of extractor (28) is extractor chamber (42), is crushed device supporting network (39)
It is divided into two parts up and down；Described grinder supporting network (39) the stainless steel, mesh diameter are 1-2 millimeters, are located at extractor
(28) at 1/3 under base portion；There is extract liquor recycling mouth (32) on extractor (28) side wall；The extract liquor recycling bin (31)
Cylindrical shape, stainless steel above connect extract liquor recycling mouth (32)；Extractor (28) base portion bottom surface is flat, and formed diameter of a circle is 5-
10 centimetres, inside fill the extract liquor being configured to by alcohol and ether according to the ratio of 1:1；It is stainless that the grinder consolidates the wing (33)
Steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 1-10 centimetres；Described grinder knife handle (38) round bar shape, lower end connect
It is connected to grinder knife (40)；The grinder knife (40) has 2-4, steel, each grinder knife (40) half moon, blade
Outwardly, blade is horizontal by 10 ° -30 ° of angle；Described grinder cylinder (36) upper outside is connected with grinder and consolidates the wing
(33), top insertion is fixed with grinder motor (34)；There is hole on the barrel of grinder cylinder (36) lower part, diameter is 0.1-1 millimeters.
Beneficial effects of the present invention are to improve citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units
It is mainly contained using Oecophylla smaragdina prothoracic gland extract and promotes the substances such as fissional juvenile hormone, divided and extracted,
It is mixed into the culture solution of citrus disease Biocontrol Strain culture, to achieve the purpose that improve citrus disease biocontrol microorganisms yield.It mentions
High citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units production is simple, and strong operability is low in cost,
Effect is obvious.
Detailed description of the invention
The following further describes the present invention with reference to the drawings.
Fig. 1 is that the ant that cuts of present invention raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units fills
Set overall structure diagram.
Fig. 2 is that the ant that cuts of present invention raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units fills
Set grooving overall structure diagram.
Fig. 3 is that the ant that cuts of present invention raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units fills
Set cutter arrangement schematic diagram.
Fig. 4 is that the ant that cuts of present invention raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units fills
Set cutter structure schematic diagram.
Fig. 5 is that the ant that cuts of present invention raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units fills
Set single grooving structural schematic diagram.
Fig. 6 is the prothoracic gland that the present invention improves citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units
Extract extracts structural schematic diagram.
1. cut ant device wall in figure, 2. stock guides, 3. feed inlets, 4. charging lids, 5. stock guide mouths, 6. striker plates, and 7.
Reclaiming plate, 8. reclaiming plates knob, 9. discharging board slots, 10. groovings, 11. negative pressure chamber, 12. exhaust tubes, 13. aspiration pump plugs, 14.
Aspiration pump power supply line, 15. aspiration pumps, 16. escape pipes, 17. cut ant device chamber, and 18. contain ant abdominal cavity, and 19. ant peduncle swaging clamps stitch,
20. ant peduncle swaging clamp, 21. contain ant cephalothorax chamber, 22. ant peduncle cutter solid plates, 23. ant peduncle cutter bodies, 24. ant peduncle cutters
Sword, 25. grooving chambers, 26. grooving walls, 27. ant peduncle cutters, 28. extractors, 29. electromagnetic ovens, 30. substrates, 31. extract liquors return
Bucket is received, 32. extract liquors recycle mouth, and 33. grinders consolidate the wing, and 34. grinder motors, 35. grinder motor power-supply wires, 36. crush
Device cylinder, 37. grinder chambers, 38. grinder knife handles, 39. grinder supporting networks, 40. grinder knives, 41. power cord of induction cooker,
42. extractor chamber, 43. cut ant device, and 44. cut ant device base portion, and 45. cut at the top of ant device, 46. extraction equipments.
Specific embodiment
Embodiment one:
As shown, raising citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units of the invention has
Body is that: Oecophylla smaragdina prothoracic gland extract process units is cut off Oecophylla smaragdina from peduncle with cutting ant device 43, leave and take head with
Chest, and then be sent in extractor 46, using Oecophylla smaragdina prothoracic gland extract, especially its main component juvenile hormone is fat-soluble
Feature is reached with the mixture of alcohol or alcohol and ether extraction prothoracic gland extract for the culture of citrus disease biocontrol microorganisms
Improve the purpose of citrus disease biocontrol microorganisms yield.Oecophylla smaragdina prothoracic gland extract process units is by cutting ant device 43 and extraction equipment
46 compositions.Cutting ant device 43 is to cut off Oecophylla smaragdina from peduncle, leaves and takes the device of head and chest, including is cut at the top of ant device
45 and 44 two parts of ant device base portion are cut, cut 45 arc surfaceds at the top of ant device, lower edge and cuts the four of 44 upper surface of ant device base portion
A side links together, and cutting 45 central distances at the top of ant device and cutting the height of 44 upper face center of ant device base portion is 5-30 lis
Rice；44 cuboid of ant device base portion is cut, length is 10-50 centimetres, and it is highly 6-30 centimetres that width, which is 8-40 centimetres, including
Cut ant device wall 1, stock guide 2, feed inlet 3, charging lid 4, stock guide mouth 5, striker plate 6, reclaiming plate 7, reclaiming plate knob 8,
Discharge board slot 9, grooving 10, negative pressure chamber 11, exhaust tube 12, aspiration pump plug 13, aspiration pump power supply line 14, aspiration pump 15, outlet
Pipe 16 cuts ant device chamber 17, contains ant abdominal cavity 18, ant peduncle swaging clamp seam 19, ant peduncle swaging clamp 20, contains ant cephalothorax chamber 21, ant peduncle
Cutter solid plate 22, ant peduncle cutter body 23, ant peduncle cutoff edge 24, grooving chamber 25, grooving wall 26, ant peduncle cutter 27.Cut ant
Device wall 1 is to cut the outer wall of ant device 43, stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 1-10 millimeters.It cuts
Ant device chamber 17 is the cavity surrounded by cutting ant device wall 1, including cutting ant device top 45 and cutting inside ant device base portion 44
Space.Stock guide 2, which is located at, to be cut among 45 at the top of ant device, stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of
0.5-5 millimeters, width is 1-3 centimetres, and the opening that is surrounded of centre is known as stock guide mouth 5, the plane where stock guide mouth 5 with cut
45 intersection points that are central and cutting line segment between 44 upper face center of ant device base portion are the midpoint of the line segment, stock guide at the top of ant device
Angle between 2 and the plane at 5 place of stock guide mouth is 30 ° -45 °, i.e., the oblique top of stock guide 2 is stood where stock guide mouth 5
Plane on, 2 neighboring of stock guide, which is fixed on, cuts 45 inner surfaces at the top of ant device, such setting so that from feed inlet 3 into
Carrying out Oecophylla smaragdina can be introduced on grooving 10.Feed inlet 3 cuts ant device top 45 and cuts ant device close to center 2-5 centimeters
Oblong openings on wall 1, length are 2-10 centimetres, and width is 1.5-8 centimetres, are to be sent into Oecophylla smaragdina to cut opening for ant device 43
Mouthful.Feeding lid 4 is the lid for sealing feed inlet 3, stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 0.5-5
Millimeter, length are 2.2--10.2 centimetres, and width is 1.52-8.2 centimetres, and top edge is fixed on 3 upper limb of feed inlet by hinge and cuts
The outer surface of ant device wall 1, lower edge and both side edges are free, raise charging lid 4, and charging counter be placed on of lid 4 is cut ant device wall 1
Outer surface on, feed inlet 3 can be exposed, convenient for from there Jiang Oecophylla smaragdina be added cut in ant device 43.Striker plate 6 is grooving
10 upper surface peripheries and the plate profile structure for cutting connection between 44 upper surface periphery of ant device base portion, stainless steel, plastics matter, aluminium
Alloy matter or pottery, with a thickness of 0.5-5 millimeters, the angle between the plane where 10 upper surface of grooving is 30 ° -45 °, i.e.,
The oblique top of striker plate 6 is stood in the plane where grooving 10, on the one hand Oecophylla smaragdina can be allowed to enter grooving chamber 25 by gravity
In, it on the other hand can will cut at the top of ant device 45 and cut ant device base portion 44 and separate, and two parts up and down be formed, to guarantee Huang Suede
Ant enters in grooving chamber 25 under the suction that aspiration pump 15 is formed by suction.Grooving 10 is Jiang Oecophylla smaragdina from chest and abdomen
The device cut off between portion, stainless steel, plastics matter, aluminium alloy matter or pottery, length are 8-40 centimetres, and width is 5-35 lis
Rice, is highly 5-25 centimetres, including reclaiming plate 7, reclaiming plate knob 8, discharging board slot 9, negative pressure chamber 11, exhaust tube 12, aspiration pump
Plug 13, aspiration pump 15, escape pipe 16, contains ant abdominal cavity 18, ant peduncle swaging clamp seam 19, ant peduncle swaging clamp at aspiration pump power supply line 14
20, ant cephalothorax chamber 21, ant peduncle cutter solid plate 22, ant peduncle cutter body 23, ant peduncle cutoff edge 24, grooving chamber 25, grooving are contained
Wall 26, ant peduncle cutter 27.Reclaiming plate 7 is the plate positioned at 10 bottom center of grooving tool groove, stainless steel, plastics matter, aluminium
Alloy matter or pottery, with a thickness of 2-20 millimeters, length is 8-40 centimetres, and width is 5-30 centimetres, when production, first uses cross section
Frame is made in batten for 2-20 millimeters of squares of side length, and then bottom nails on the stainless steel that an aperture is 0.1-0.5 millimeters
Silk screen, stainless steel cloth space above are the groove of reclaiming plate 7；A drawing is followed closely in the outer surface center of 7 broadside of reclaiming plate
Hand, i.e. reclaiming plate knob 8, to pull out reclaiming plate 7 from discharging board slot 9.When entrance 25 Oecophylla smaragdina of grooving chamber is by ant
After peduncle cutter 27 cuts off and is pushed into the groove of reclaiming plate 7 by ant peduncle cutter body 23, under external force, with reclaiming plate 7
It is drawn out, and the cephalothorax of Oecophylla smaragdina is collected in case extracting used in prothoracic gland extract.Discharging board slot 9 is to cut ant device
Base portion 44 holds the space of reclaiming plate 7, there is the opening being adapted with 7 cross section size of reclaiming plate on cutting ant device wall 1,
Reclaiming plate 7 can be stuffed into or pull out in opening.Negative pressure chamber 11 is located at 10 bottommost of grooving, and the above is reclaiming plate 7, and side connects
Then exhaust tube 12, due to pumping, so that the pressure in negative pressure chamber 11 declines, pass through 7 He of reclaiming plate when aspiration pump 15 works
Grooving chamber 25, which is twitched, is located at 10 top Oecophylla smaragdina of grooving, and Shi Oecophylla smaragdina is pumped into grooving chamber 25.15 structure of aspiration pump and
For principle with well known aspiration pump, one end connects exhaust tube 12, and the other end connects escape pipe 16, passes through pumping by aspiration pump power supply line 14
Air pump plug 13 obtains electric energy from the external world, carries control panel on aspiration pump 15, can control the strength and switch of pumping.Exhaust tube
12 be rubber mass hose, and lumen diameter is 3-10 millimeters, and 1-5 millimeters of wall thickness, tube wall is interior to be supported by coil spring, is on the one hand made
Obtaining exhaust tube 12 has elasticity and toughness, and 12 lumen of exhaust tube is on the other hand made to remain unimpeded.Escape pipe 16 is rubber
Matter hose, lumen diameter are 5-20 millimeters, and 1-5 millimeters of wall thickness, tube wall is interior to be supported by coil spring, on the one hand makes escape pipe
16 have elasticity and toughness, and 16 lumen of escape pipe is on the other hand made to remain unimpeded.The stainless steel of grooving wall 26, plastics
Matter, aluminium alloy matter or pottery, with a thickness of 1-10 millimeters, lattice-shaped links together, and the space surrounded is grooving chamber 25.It cuts
Vallecular cavity more than 25 is a, arranged in rows, is separated between two adjacent cutback chambers 25 by grooving wall 26, and the length of each grooving chamber 25 is 6-
8 millimeters, it is highly 4-20 centimetres that width, which is 3-5 millimeters, long to one ant peduncle swaging clamp of setting at 2/3 in each grooving chamber 25
20, all ant peduncle swaging clamps 20 of every row are located point-blank, and grooving chamber 25 is divided by ant peduncle swaging clamp 20 contains ant abdominal cavity
18 and 21 two parts of ant cephalothorax chamber are contained, is connected between two parts by ant peduncle swaging clamp seam 19.Ant peduncle swaging clamp 20 includes left and right two
Part, the gap between two parts are that the ant peduncle swaging clamp that width is 0.5-1 millimeter stitches 19 , Oecophylla smaragdinas in the suction work of aspiration pump 15
Under, the peduncle between chest and abdomen is stuck in ant peduncle swaging clamp seam 19, and abdomen is shorter, stays in and accounts for 25 volume 1/ of grooving chamber
In 3 Sheng ant abdominal cavity 18, head and chest are longer, stay in the Sheng ant cephalothorax chamber 21 for accounting for 25 volume 2/3 of grooving chamber.Ant peduncle card
20 free edge of knife has an inclined-plane to the direction of grooving wall 26 in ant peduncle swaging clamp seam 19, to guarantee that ant peduncle swaging clamp 20 is free
Edge blade-like, convenient for being cut Oecophylla smaragdina from peduncle.It is ant peduncle cutter more than 27, corresponding with grooving chamber 25, i.e., each
Ant peduncle cutter 27 corresponds to a grooving chamber 25, including ant peduncle cutter solid plate 22, ant peduncle cutter body 23 and ant peduncle are cut
Blade 24 is to put in grooving chamber 25 in Sheng 21 side of ant cephalothorax chamber along ant peduncle swaging clamp 20, is combined into and cuts with ant peduncle swaging clamp 20
Knife, so that Oecophylla smaragdina be cut off from peduncle.Ant peduncle cutter solid plate 22 is the plate of cuboid, and side edge center is connected to
Exhaust tube 12, intermediate empty, exhaust tube 12 separates branch pipe in the hollow part of ant peduncle cutter solid plate 22, and each branch pipe is one corresponding
Ant peduncle cutter 27, branch pipe lumen are come round with the space in ant peduncle cutter body 23, and a piston is arranged in branch pipe lumen, living
The flanking central filled in ant peduncle cutter body 23 is linked together by a steel wire and 24 base portion of ant peduncle cutoff edge, ant peduncle
A spring is connected between cutoff edge 24 and ant peduncle cutter solid plate 22, crust is made by silica gel in ant peduncle cutter body 23, because
There are spring and steel wire in inside, can stretch.As exhaust tube 12 is evacuated, piston is pulled to outer, drive 24 quilt of ant peduncle cutoff edge
The direction of grooving chamber 25 is pulled to out, meanwhile, spring of extruding, the spring potential energy made increases, and helical form tightens ant peduncle cutoff edge 24,
After being retracted to specific position, 24 helicla flute of ant peduncle cutoff edge disappears, instead straight trough, and under the action of the spring, ant peduncle is cut
Blade 24 puts in grooving chamber 25 in Sheng 21 side of ant cephalothorax chamber along ant peduncle swaging clamp 20 rapidly, to play the function of shearing peduncle
Can, in this way, ant peduncle cutoff edge 24 is periodically drawn as exhaust tube 12 constantly provides energy and spring collective effect
It rises, pop-up, plays shear action, meanwhile, the circular configuration of 24 base portion of ant peduncle cutoff edge can also will scale off Huang Suede
In the groove of the head of ant and chest push-in reclaiming plate 7.Extraction equipment 46 is to extract Oecophylla smaragdina prothoracic gland contained substance
Device, including extractor 28, electromagnetic oven 29, substrate 30, extract liquor recycling bin 31, extract liquor recycling mouth 32, grinder consolidate the wing
33, grinder motor 34, grinder motor power-supply wire 35, grinder cylinder 36, grinder chamber 37, grinder knife handle 38, grinder
Supporting network 39, grinder knife 40, power cord of induction cooker 41, extractor chamber 42.Substrate 30 is that extraction equipment 46 plays a supportive role
Cuboid plate, stainless steel, plastics matter, aluminium alloy matter or pottery, with a thickness of 1-10 centimetres.28 nature of glass of extractor, base
Portion is substantially spherical in shape, and diameter is 10-30 centimetres；Neck is cylindrical, and it is highly 5-10 centimetres that diameter, which is 5-10 centimetres,.Extractor
Space inside 28 is extractor chamber 42, and pulverized device supporting network 39 is divided into two parts up and down, and top is will to acquire the Huang come
The head of Suede ant and chest crush, the part mixed with extract liquor, and lower part is to hold extract liquor to dissolve head and chest extracting
The part of object.The stainless steel of grinder supporting network 39, mesh diameter are 1-2 millimeters, are located at 1/3 under 28 base portion of extractor, use
In support grinder.There is an extract liquor recycling mouth 32 in 28 one side wall of extractor, is crushed Jiang the head of Oecophylla smaragdina and chest
Afterwards after active material dissolution, is heated by electromagnetic oven 29, extract liquor is allowed to evaporate, separated with prothoracic gland extract, the extraction after evaporation
Take liquid in the cooled liquids recovery that becomes of extract liquor recycling mouth 32 into extract liquor recycling bin 31, in case continuing to use next time.Extraction
Liquid recycling bin 31 is cylinder-shaped, stainless steel, above connects extract liquor recycling mouth 32.Electromagnetic oven 29 is well known electromagnetic oven, in electromagnetic oven
After power supply line 41 introduces electric energy from the external world, extract liquor can be heated, so that extract is separated with extract liquor.28 base portion of extractor
Bottom surface is flat, and formed diameter of a circle is 5-10 centimetres, for extractor 28 to be smoothly placed on to the electromagnetic oven in substrate 30
On 29, the extract liquor being configured to by alcohol and ether according to the ratio of 1:1 is inside filled.It is to fix grinder that grinder, which consolidates the wing 33,
Spheric plate on 28 neck of extractor, stainless steel, plastics matter, aluminium alloy matter or pottery are used with a thickness of 1-10 centimetres
It is on grinder supporting network 39 in guarantee grinder.Grinder motor 34 is with well known motor, by grinder motor power-supply wire
35 obtain electric energy from the external world, and grinder knife handle 38 and grinder knife 40 is driven to rotate in grinder chamber 37, reach and crush Huang Suede
The purpose on ant head and chest.38 round bar shape of grinder knife handle, lower end are connected with grinder knife 40, can be by grinder motor 34
Rotor rotation is synchronized to grinder knife 40, so that the quick rotation in grinder cylinder 36 of grinder knife 40, crushes Oecophylla smaragdina head
And chest.Grinder knife 40 has a 2-4, steel, each half moon, and outwardly, blade is horizontal by 10 ° -30 ° of folder for blade
Angle.36 upper outside of grinder cylinder is connected with grinder and consolidates the wing 33, and top insertion is fixed with grinder motor 34, when Jia Oecophylla smaragdina,
Grinder motor 34 is taken away, Oecophylla smaragdina can be added, when crushing, grinder motor 34 is inserted at the top of grinder cylinder 36 solid
Fixed, after energization, i.e., available grinder knife 40 crushes Oecophylla smaragdina head and chest；There is hole on 36 lower part barrel of grinder cylinder, directly
Diameter is 0.1-1 millimeters, and extract liquor can enter in grinder cylinder 36 from this some holes, connect with smashed Oecophylla smaragdina head and chest
Touching, for guaranteeing that smashed Oecophylla smaragdina head and chest are soaked in extract liquor.
The application method of Oecophylla smaragdina prothoracic gland extract: taking 1mg Oecophylla smaragdina prothoracic gland extract, and 1000ml pure water is added
In, extract suspension is made, according to the ratio of 1:1000, citrus disease biological and ecological methods to prevent plant disease, pests, and erosion bacteria culture fluid is added in extract suspension
In, it is then placed in 37 DEG C of shaking tables and cultivates 16-20 hours, shaking speed 300rpm, measure bacterium growing state list with calculating instrument
Bacterium number in the volume of position.
Using effect comparative experiments: to verify practicability of the invention, i.e. raising citrus disease biocontrol microorganisms yield Huang Suede
Can ant prothoracic gland extract process units improve citrus disease biocontrol microorganisms yield? improving citrus disease biocontrol microorganisms yield
In Oecophylla smaragdina prothoracic gland extract process units application process, by many experiments, the results are shown in Table 1:
Influence of the 1 Oecophylla smaragdina prothoracic gland extract of table to citrus disease biocontrol microorganisms culture yield (cfu/mL)
As can be seen from the above results, Oecophylla smaragdina prothoracic gland extract is added in the culture medium of citrus disease biocontrol microorganisms
Afterwards, bacterium amount obtained is significantly more than and is not added with the bacterium amount that the culture medium of Oecophylla smaragdina prothoracic gland extract obtains in the unit time,
Thus improving citrus disease biocontrol microorganisms yield Oecophylla smaragdina prothoracic gland extract process units can be improved citrus disease biological and ecological methods to prevent plant disease, pests, and erosion
Bacterium yield.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it will be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this hairs
Bright principle, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these variations
It all fall within the protetion scope of the claimed invention with improvement, its is equivalent by appended claims for the claimed scope of the invention
Object defines.
Claims (3)
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| CN201710077055.6A CN106818884B (en) | 2017-02-14 | 2017-02-14 | Production device for prothymus gland extract of the yellow antler to increase the yield of biocontrol bacteria against citrus diseases |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710077055.6A CN106818884B (en) | 2017-02-14 | 2017-02-14 | Production device for prothymus gland extract of the yellow antler to increase the yield of biocontrol bacteria against citrus diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093890A (en) * | 1993-12-10 | 1994-10-26 | 大姚县金碧造纸厂 | The Processes and apparatus of producing tobacco flake in method similar to papermaking |
| CN103563852A (en) * | 2012-07-27 | 2014-02-12 | 财团法人工业技术研究院 | Automatic insect body fluid collection machine |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093890A (en) * | 1993-12-10 | 1994-10-26 | 大姚县金碧造纸厂 | The Processes and apparatus of producing tobacco flake in method similar to papermaking |
| CN103563852A (en) * | 2012-07-27 | 2014-02-12 | 财团法人工业技术研究院 | Automatic insect body fluid collection machine |
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