CN106813962A - The preparation method that a kind of zooplankter liquid-based seals sample processed - Google Patents
The preparation method that a kind of zooplankter liquid-based seals sample processed Download PDFInfo
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- CN106813962A CN106813962A CN201710064649.3A CN201710064649A CN106813962A CN 106813962 A CN106813962 A CN 106813962A CN 201710064649 A CN201710064649 A CN 201710064649A CN 106813962 A CN106813962 A CN 106813962A
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- coloured
- acrylic board
- acrylic
- zooplankter
- coloured acrylic
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The preparation method that a kind of zooplankter liquid-based seals sample processed, is related to zoological specimens.Make 2 a of coloured acrylic board 1, the 2 colourless b of acrylic circular slab 1,1 piece of coloured d of acrylic block 1 of 1 c and 1 piece of coloured acrylic board；1 a is identical with 1 c sizes, and round hole is provided with the side of 1 a and 1 c, and round hole is concentric circles, and the sides of 1 c mono- are provided with convex breach, and the convex breach on 1 d and 1 c agrees with, and the diameter of 1 b is identical with the round hole diameter on 1 a；Centered on 1 c, 1 a is sticked in 1 c both sides；Edge cementing between the round hole of 1 a and 1 c, then 1 b is sticked obtain sample storage pool in 1 c both sides；Convex breach injection sample storage pool by fixer from the left of 1 c, adds zooplankter sample, then 1 d is filled in into the convex breach on the left of 1 c, seals, and obtains final product zooplankter liquid-based and seals sample processed.
Description
Technical field
The present invention relates to a kind of zooplankter sample, the making side of sample processed is sealed more particularly, to a kind of zooplankter liquid-based
Method.
Background technology
Zooplankter is the important biomolecule monoid of fresh water and marine ecosystem, lacks flourishing action organ, autonomous fortune
Kinetic force is very weak, and general individual is smaller, it is necessary to can just be seen clearly their construction by means of Stereo microscope or biomicroscope.Mesh
The preceding zooplankter sample for micro- sem observation is mainly the liquid-based specimens for being stored in the preservations such as specimen bottle formaldehyde fixer,
Drawn during observation to culture dish or slide and observed, after observation is finished, sample returns bottle or discarding.This process exists following
Problem：1. sample during transfer, observation it is damaged, lose；2. the volatilization of fixer, and the fixer for volatilizing is to observation
The infringement of person's respiratory system；3. sample is observed be not as convenient as plant slide, and process is cumbersome.And traditional plant encapsulation slide is only suitable for
Make the portion of tissue organ of small-sized, microzooplankton and big-and-middle-sized zooplankter.
Chinese patent CN104048868A discloses a kind of zooplankter microscope slide liquid-based specimens manufacturing technology, with concave slide
Be carrier, using formalin fix, brazilwood extract dyeing, sour alcohol color separation, running water oil blackeite, dehydration of alcohol, glycerine it is transparent, in
Property the process such as gummy mounting, make zooplankter microscope slide sample, realize it is long-term preserve zooplankter material while,
Preservation material shape structure to greatest extent is realized, and improves the definition and contrast of sample observation.
The content of the invention
Liquid-based specimens it is an object of the invention to be directed to the existing zooplankter sample storage for micro- sem observation
The above mentioned problem of presence, there is provided be capable of achieving the long-term preservation of zooplankter sample, convenient classification observation makes simple, not fragile
The preparation method that a kind of broken zooplankter liquid-based seals sample processed.
The present invention is comprised the following steps：
1) 2 coloured acrylic board 1-a, 2 colourless acrylic circular slab 1-b, 1 piece of coloured acrylic board 1-c and 1 are made
The coloured acrylic block 1-d of block, wherein, the appearance and size of 2 coloured acrylic board 1-a acrylic board 1-cs coloured with 1 piece is identical,
Round hole, all circles are equipped with 2 sides and 1 piece of side of coloured acrylic board 1-c of coloured acrylic board 1-a
Perforate is concentric circles, and the round hole diameter on coloured acrylic board 1-c is straight less than the round hole on coloured acrylic board 1-a
Footpath a, side of coloured acrylic board 1-c is provided with convex breach, on coloured acrylic block 1-d and coloured acrylic board 1-c
Convex breach agrees with, the round hole diameter on 2 diameters and 2 coloured acrylic board 1-a of colourless acrylic circular slab 1-b
It is identical；
2) centered on 1 piece of coloured acrylic board 1-c, 2 coloured acrylic board 1-a are binded at coloured sub- gram with glue
The both sides of power plate 1-c；
3) between 2 round holes and 1 piece of round hole of coloured acrylic board 1-c of coloured acrylic board 1-a
Lower edges spreading glue, then 2 colourless acrylic circular slab 1-b bondings are obtained into sample in the both sides of coloured acrylic board 1-c
Storage pool；
4) the convex breach injection sample storage pool by fixer from the left of coloured acrylic board 1-c, and add to be made
The zooplankter sample of work, then coloured acrylic block 1-d is filled in into the convex breach on the left of coloured acrylic board 1-c, and outside
Side uses sealant sealing, finally pastes label on the outside of coloured acrylic board 1-a, that is, complete zooplankter liquid-based and seal sample processed
Making.
The thickness of the coloured acrylic board 1-a can be 1mm, the thickness of colourless acrylic circular slab 1-b can 2.5mm, have
The thickness of color acrylic board 1-c can be 5mm, and the thickness of coloured acrylic block 1-d can be 5mm.
The glue can use acrylic glue special.
The fixer can use the fresh water or seawater for 5% formaldehyde containing mass percentage concentration；The fresh water or seawater can
Using filtering fresh water or seawater.
The present invention is made using acrylic material, and with two-side window, sealing filling therebetween contains 5% concentration formaldehyde
Filtering fresh water or seawater are used to preserve zooplankter, can be under magnifying glass, stereomicroscope and biomicroscope to sealing sample processed
Observed, the features such as with simple, non-breakable, two-sided observation is made, it is particularly possible to carry out different angles by rocking sample
The observation of degree, and avoid injury of the formaldehyde to human body.
Brief description of the drawings
Fig. 1 is that the zooplankter liquid-based that the embodiment of the present invention makes seals sample structure composition decomposing schematic representation processed.
Fig. 2 is the structural representation of coloured acrylic board 1-a of the invention.
Fig. 3 is the structural representation of colourless acrylic circular slab 1-b of the invention.
Fig. 4 is the structural representation of coloured acrylic board 1-c of the invention.
Fig. 5 is the structural representation of coloured acrylic block 1-d of the invention.
Specific embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated.In Fig. 1~5, relative dimensions can root
Voluntarily adjusted according to the size of packaged sample, microscope carrier.
The present invention is comprised the following steps：
1) 2 coloured acrylic board 1-a, 2 colourless acrylic circular slab 1-b, 1 piece of coloured acrylic board 1-c and 1 are made
The coloured acrylic block 1-d of block, wherein, the appearance and size of 2 coloured acrylic board 1-a acrylic board 1-cs coloured with 1 piece is identical,
Round hole, all circles are equipped with 2 sides and 1 piece of side of coloured acrylic board 1-c of coloured acrylic board 1-a
Perforate is concentric circles, and the round hole diameter on coloured acrylic board 1-c is straight less than the round hole on coloured acrylic board 1-a
Footpath a, side of coloured acrylic board 1-c is provided with convex breach, on coloured acrylic block 1-d and coloured acrylic board 1-c
Convex breach agrees with, the round hole diameter on 2 diameters and 2 coloured acrylic board 1-a of colourless acrylic circular slab 1-b
It is identical；
2) centered on 1 piece of coloured acrylic board 1-c, 2 coloured acrylic board 1-a are binded at coloured sub- gram with glue
The both sides of power plate 1-c；
3) between 2 round holes and 1 piece of round hole of coloured acrylic board 1-c of coloured acrylic board 1-a
Lower edges spreading glue, then 2 colourless acrylic circular slab 1-b bondings obtain sample storage in the both sides of coloured acrylic board 1-c
Deposit pond；
4) the convex breach injection sample storage pool by fixer from the left of coloured acrylic board 1-c, and add to be made
The zooplankter sample of work, then coloured acrylic block 1-d is filled in into the convex breach on the left of coloured acrylic board 1-c, and outside
Side uses sealant sealing, finally pastes label on the outside of coloured acrylic board 1-a, that is, complete zooplankter liquid-based and seal sample processed
Making.
The thickness of the coloured acrylic board 1-a can be 1mm, the thickness of colourless acrylic circular slab 1-b can 2.5mm, have
The thickness of color acrylic board 1-c can be 5mm, and the thickness of coloured acrylic block 1-d can be 5mm.
The glue can use acrylic glue special.
The fixer can use the fresh water or seawater for 5% formaldehyde containing mass percentage concentration；The fresh water or seawater can
Using filtering fresh water or seawater.
Claims (5)
1. the preparation method that a kind of zooplankter liquid-based seals sample processed, it is characterised in that it is comprised the following steps：
1) 2 coloured acrylic boards (1-a), 2 colourless acrylic circular slabs (1-b), 1 piece of coloured acrylic board (1-c) are made
With 1 piece of coloured acrylic block (1-d), wherein, 2 coloured acrylic boards (1-a) and 1 piece of profile of coloured acrylic board (1-c)
Size is identical, and circle is equipped with 2 sides and 1 piece of side of coloured acrylic board (1-c) of coloured acrylic board (1-a)
Perforate, all round holes are concentric circles, and the round hole diameter on coloured acrylic board (1-c) is less than coloured acrylic board
Round hole diameter on (1-a) a, side of coloured acrylic board (1-c) is provided with convex breach, coloured acrylic block (1-
D) agree with the convex breach on coloured acrylic board (1-c), the diameter of 2 colourless acrylic circular slabs (1-b) is coloured with 2
Round hole diameter on acrylic board (1-a) is identical；
2) centered on 1 piece of coloured acrylic board (1-c), 2 coloured acrylic boards (1-a) are binded at coloured sub- gram with glue
The both sides of power plate (1-c)；
3) between 2 round holes and 1 piece of round hole of coloured acrylic board (1-c) of coloured acrylic board (1-a)
Lower edges spreading glue, then 2 colourless acrylic circular slab (1-b) bondings are obtained in the both sides of coloured acrylic board (1-c)
Sample storage pool；
4) the convex breach injection sample storage pool by fixer from the left of coloured acrylic board (1-c), and add to be made
Zooplankter sample, then coloured acrylic block (1-d) is filled in into the convex breach on the left of coloured acrylic board (1-c), and
Outside uses sealant sealing, finally pastes label in coloured acrylic board (1-a) outside, that is, complete zooplankter liquid-based envelope system
The making of sample.
2. a kind of preparation method that zooplankter liquid-based seals sample processed as claimed in claim 1, it is characterised in that the coloured Asia
The thickness of gram force plate (1-a) is 1mm, the thickness 2.5mm of colourless acrylic circular slab (1-b), the thickness of coloured acrylic board (1-c)
It is 5mm to spend, and the thickness of coloured acrylic block (1-d) is 5mm.
3. the preparation method that a kind of zooplankter liquid-based seals sample processed as claimed in claim 1, it is characterised in that the glue is adopted
Use acrylic glue special.
4. a kind of preparation method that zooplankter liquid-based seals sample processed as claimed in claim 1, it is characterised in that the fixer
Use fresh water or seawater for 5% formaldehyde containing mass percentage concentration.
5. the preparation method that a kind of zooplankter liquid-based seals sample processed as claimed in claim 4, it is characterised in that the fresh water or
Seawater is using filtering fresh water or seawater.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710064649.3A CN106813962B (en) | 2017-02-04 | 2017-02-04 | A kind of production method that zooplankter liquid-based seals sample processed |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710064649.3A CN106813962B (en) | 2017-02-04 | 2017-02-04 | A kind of production method that zooplankter liquid-based seals sample processed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106813962A true CN106813962A (en) | 2017-06-09 |
| CN106813962B CN106813962B (en) | 2019-05-10 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710064649.3A Expired - Fee Related CN106813962B (en) | 2017-02-04 | 2017-02-04 | A kind of production method that zooplankter liquid-based seals sample processed |
Country Status (1)
| Country | Link |
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| CN (1) | CN106813962B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109357922A (en) * | 2018-10-26 | 2019-02-19 | 珠江水利委员会珠江水利科学研究院 | A kind of body before zooplankter sample identification restores preprocess method |
| CN110692563A (en) * | 2019-08-02 | 2020-01-17 | 浙江海洋大学 | Reshaping method before copepod sample identification |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06324038A (en) * | 1993-05-14 | 1994-11-25 | Fuji Photo Film Co Ltd | Analysis slide and manufacture thereof |
| US20120227478A1 (en) * | 2011-03-08 | 2012-09-13 | Asuka Mukai | Liquid Living Body Phantom and Method of Making the Same |
| CN202600250U (en) * | 2012-02-07 | 2012-12-12 | 贾立锋 | Specimen holder used for optical microscope refrigeration objective table |
| CN203376196U (en) * | 2013-08-13 | 2014-01-01 | 青岛农业大学 | Liquid-based specimen manufacturing slide |
| CN103698184A (en) * | 2013-12-28 | 2014-04-02 | 青岛大学 | Device for manufacturing parasite egg slide specimen for long-term use |
-
2017
- 2017-02-04 CN CN201710064649.3A patent/CN106813962B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06324038A (en) * | 1993-05-14 | 1994-11-25 | Fuji Photo Film Co Ltd | Analysis slide and manufacture thereof |
| US20120227478A1 (en) * | 2011-03-08 | 2012-09-13 | Asuka Mukai | Liquid Living Body Phantom and Method of Making the Same |
| CN202600250U (en) * | 2012-02-07 | 2012-12-12 | 贾立锋 | Specimen holder used for optical microscope refrigeration objective table |
| CN203376196U (en) * | 2013-08-13 | 2014-01-01 | 青岛农业大学 | Liquid-based specimen manufacturing slide |
| CN103698184A (en) * | 2013-12-28 | 2014-04-02 | 青岛大学 | Device for manufacturing parasite egg slide specimen for long-term use |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109357922A (en) * | 2018-10-26 | 2019-02-19 | 珠江水利委员会珠江水利科学研究院 | A kind of body before zooplankter sample identification restores preprocess method |
| CN109357922B (en) * | 2018-10-26 | 2021-01-05 | 珠江水利委员会珠江水利科学研究院 | Organism recovery pretreatment method before identification of zooplankton sample |
| CN110692563A (en) * | 2019-08-02 | 2020-01-17 | 浙江海洋大学 | Reshaping method before copepod sample identification |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106813962B (en) | 2019-05-10 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
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