CN106801062A - It is a kind of to turn the method that NtZFP8 genes improve Plant Tissue Breeding differentiation efficiency - Google Patents

It is a kind of to turn the method that NtZFP8 genes improve Plant Tissue Breeding differentiation efficiency Download PDF

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Publication number
CN106801062A
CN106801062A CN201710154117.9A CN201710154117A CN106801062A CN 106801062 A CN106801062 A CN 106801062A CN 201710154117 A CN201710154117 A CN 201710154117A CN 106801062 A CN106801062 A CN 106801062A
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plant
ntzfp8
recombinant
genes
callus
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徐莺
时小东
田银帅
王灵慧
陈放
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Sichuan University
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Botany (AREA)
  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
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  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Turn the method that NtZFP8 genes improve Plant Tissue Breeding differentiation efficiency the invention discloses a kind of.The invention provides a kind of genetic fragment, its nucleotide sequence is as shown in SEQ ID NO.1.The invention also discloses the recombinant vector comprising forementioned gene fragment, recombinant bacterium and their purposes.The present invention significantly improves the power of regeneration of plant explant and callus by being transferred to NtZFP8 genetic fragments in plant, reduces regrowth and obtains the cycle, obtains the regeneration plant with value.The method can significantly improve the power of regeneration of plant explant and callus, with potential application value and economic benefit.

Description

It is a kind of to turn the method that NtZFP8 genes improve Plant Tissue Breeding differentiation efficiency
Technical field
The present invention relates to genetic engineering field, and in particular to one kind turns NtZFP8 genes and improves Plant Tissue Breeding differentiation effect The method of rate.
Background technology
Plant tissue culture technique is the embodiment of cellular omnipotency.With somatic embryo genesis mechanism research deeply and The fast development of association area, plant tissue culture technique is not only in the quick proliferation of forest, crops and garden crop It is used widely, is also played an important role in genetic engineering breed of variety.Plant Tissue Breeding mainly passes through two Approach：One directly induces generation for explant；The second is indirect induction occurs, i.e., by somatic induction into embryo callus subculture group Knit, callus breaks up sprout again, obtain regeneration plant.Differentiation efficiency is to weigh the important finger of plant tissue culture technique efficiency Mark.
The differentiation of explant and callus is a biological process for complexity, it is necessary to some specific physics and chemistry The factor is started and is maintained, such as the genotype of recipient plant, explant type and physiological status, culture medium composition and plant Hormone composition etc..Studied such as in paddy rice and found, different genotype paddy rice, its callus differentiation capability is different, japonica rice is bright It is aobvious to be better than long-grained nonglutinous rice (Lin Y J, Zhang Q.Optimising the tissue culture conditions for high efficiency transformation of indica rice[J].Plant Cell Reports,2005,23(8): 540-547.), while also producing influence (Tie W, Zhou F, Wang L, et on Indica Rice Callus transgene efficiency al.Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation:lessons from transformation assays and genome-wide expression profiling[J].Plant Molecular Biology,2012, 78(1):1-18.).Explant and callus differentiation efficiency problem increased the week of Plant Tissue Breeding batch production scale breeding Phase, improve production cost.
Forefathers change the measure such as medium component and improvement culture environment and improve differentiation capability by adjusting hormone combination (Zhu Keming, Tao Huimin, Xu Shuo, wait rice callus to break up and regeneration progress [J] seeds, and 2016 (11):55-59.).With The development of Protocols in Molecular Biology, carry out recipient plant transformation by plant biological engineering has turned into so as to improve power of regeneration One new research direction.
The content of the invention
It is an object of the invention to provide a kind of new gene, and plant tissue is improved by way of by its transgenosis Culture differentiation efficiency.
Present invention firstly provides a kind of genetic fragment, its nucleotide sequence is as shown in SEQ ID NO.1.
Present invention also offers a kind of recombinant vector, it includes the nucleotide sequence shown in SEQ ID NO.1.Preferably, The recombinant vector is restructuring pCAMBIA1301 carriers.
Present invention also offers a kind of recombinant bacterium, it includes foregoing recombinant vector preferably, and the recombinant bacterium is restructuring agriculture Bacillus, more preferably recombinational agrobacterium EHA105.
Present invention also offers a kind of albumen, its amino acid sequence as shown in SEQ ID NO.2
The explant of plant is being improved present invention also offers foregoing genetic fragment, recombinant vector, recombinant bacterium, albumen And/or the purposes in the differentiation efficiency of callus.
Wherein, the plant is tobacco.
Present invention also offers a kind of method for improving Plant Tissue Breeding differentiation efficiency, foregoing genetic fragment, weight are taken Group carrier, recombinant bacterium, are transferred in plant, obtain plant or the seed of stabilization expression foregoing proteins, you can.
Wherein, the plant is tobacco.
A kind of method for turning NtZFP8 raising Plant Tissue Breeding regeneration rates that the present invention is provided, using the tobacco present invention NtZFP8 genetic fragments, obtain the transgenic line of stabilization heredity, and checking test shows that NtZFP8 genes can be to plant Explant and callus differentiation play an important role, and breaking up the regeneration bud for obtaining can develop into plant, therefore can be described Gene and recombinant plasmid are applied in breed breeding and tissue cultures batch production, from this new way service of plant genetic engineering In application and production.
Obviously, the above of the invention, according to the ordinary technical knowledge and customary means of this area, is not departing from Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification of other diversified forms can also be made, is replaced or is changed.
The specific embodiment of form, remakes further specifically to the above of the invention by the following examples It is bright.But this scope for being interpreted as above-mentioned theme of the invention should not be only limitted to following example.It is all based on the above of the present invention The technology realized belongs to the scope of the present invention.
Brief description of the drawings
The electrophoretogram that Fig. 1 is expanded for the PCR of NtZFP8 genes, wherein M swimming lanes are molecular weight marker (AL2000DNAMarker, purchased from Ai Delai companies), 1-2 swimming lanes are NtZFP8 amplifications.
Fig. 2 is the electrophoretogram for turning NtZFP8 gene Agrobacterium bacterium colonies PCR, and wherein M swimming lanes are molecular weight marker (AL2000DNAMarker, purchased from Ai Delai companies), 1-6 swimming lanes are Agrobacterium bacterium solution PCR amplifications.
The qualification result of Fig. 3 NtZFP8 gene expression doses, wherein, 1 contrasts for wild type, and 2 and 3 test for overexpression Group.
Fig. 4 is the callus differentiation figure of wild type and overexpression NtZFP8 tobaccos, and wherein A is contrasted for wild type, and B is super Expression experimental group.
Specific embodiment
If not specializing, the conventional meanses that technological means used is well known to those skilled in the art in embodiment, not The experimental methods of molecular biology that work is illustrated, equal reference《Molecular biology experiment handbook》In (Marvin's is beautiful, 2011) book Listed specific method is carried out, or is carried out according to kit and product description.
The separation of the NtZFP8 genes of the present invention of embodiment 1 and the structure of transgenic line
The present embodiment is mainly the acquisition of NtZFP8 genes, and method prepared by vector construction and transgenic line is briefly introduced It is as follows.Tobacco background material used is your cigarette 6 in experiment, by Sichuan University's Life Science College development of plants Physiological Experiment room Preserve.
(1) structure of genetic fragment and carrier
0.5 gram of fresh blade of tobacco is weighed, tobacco RNA is extracted, PrimeScript is usedTM RT reagent Kit (RR037Q) cDNA, design primer (NtZFP8-F are synthesized：5’-ATGGACAAAACAGACAGAGAAAC-3’；NtZFP8-R：5’- TCACAAGTGTAGATCTAAACTCACAT-3 '), enter performing PCR amplification, obtain fragment (result such as Fig. 1 institutes of purpose band size Show).PCR primer is purified with glue reclaim kit (Takara, 805A), connection carrier T (Transgene Biotech, CT101- 01) bacillus coli DH 5 alpha competence, is converted with thermal shock method, conversion product is screened on the LB flat boards containing kanamycins, picking list Clone, send Chengdu Qing Ke Zi Xi Bioisystech Co., Ltd to be sequenced.
The nucleotide sequence (SEQ ID NO.1) of purpose fragment NtZFP8 genes that amplification is obtained is：
ATGGACAAAACAGACAGAGAAACTCGTGATTTCATGAACGTGGAGTCCTTCTCTCAGCTACCCTTTATT CGACCAGCACCAGCTAATAAAGAAAAAGCCATTAGGCTTTTTGGCAAAGAATTTGGTACTGCTGGTGACTCCATGGC TGCAACAGAATCCACTGAAACAAACCCTAGCCAAGAAGAAATCAAAGACCATATTAGCAACAACAGTGAGAGCAGTC GAAAATTCGAGTGCCATTATTGTTGCAGGAATTTCCCGACTTCACAAGCACTAGGAGGACATCAAAATGCTCATAAG AGAGAACGCCAGAATGCTAAAAGAGCGCATCTTCAATCTGCAATGGTACACGGTGCTTTAACAGAGGCAAATATTTA TGGAATAATGAATTACCATCGTCTTGGTAGTGCGCCAACAGCAGCTTATCATCATCACTACGCAACCAACAATATTA ACAGTGCTACTAGGTTTTATGGAAGCCATCACCATGTTAATAGTACCCCTTATAATTCTCATCAAACTCCTATAAAT GGAAGTCCTTTAGCTTTATGGAGGATTCCTGCAGCAACTAGTGTTCATCATACTTCTTCGTCTTCGTCTAATTTTGG CCGTGAACGATCCATGAATATGCAGCCATTGCCAGTATTTGCTGCTAATAATGAGGATTTCAAGCCTTCTCCAATCA TCAATAGCTCAAGTTCTCAAAGAGGGTTCGGATATGAAACAAAGGCCGGAATTAAAGATCATGTGAGTTTAGATCTA CACTTGTGA
NtZFP8 genes described in foregoing nucleotide sequence can be obtained by using the above method, it is also possible to directly be closed Into.
The plasmid containing NtZFP8 genes is extracted, double digestion, T4-DNAligase (RT406) connections pCAMBIA1301 is carried Body, after 16 DEG C of connections overnight, is transferred to recon competent escherichia coli cell and obtains transformant.The plasmid of transformant is extracted, After being converted Agrobacterium EHA105 competence, picking single bacterium colony carries out bacterium colony PCR, bacterium colony PCR results (result is as shown in Figure 2) There is the amplified production consistent with NtZFP8 gene sizes in display.
(2) preparation of genetically modified plants
Recombinational agrobacterium bacterial strain is cultivated, culture to bacterial concentration is OD600=0.6.
Tobacco leaf disc is immersed in bacterium solution, is soaked 10 minutes.It is placed in and contains 6- benzyl aminoadenine 2.0mg/L and naphthalene second In MURASHIGE SKOOG (MS) basal medium (being purchased from PhytoTech companies of the U.S.) of sour 0.5mg/L, dark place culture 2d； Be to remove unnecessary Agrobacterium, tobacco leaf disc is transferred to containing 6- benzyls aminoadenine 2.0mg/L, methyl α-naphthyl acetate 0.5mg/L and After on the MS culture mediums of cephalosporin 300mg/L.
Tobacco leaf disc is containing 6- benzyls aminoadenine 2.0mg/L, methyl α-naphthyl acetate 0.5mg/L, kanamycins 100mg/L and head After being grown two weeks on the MS culture mediums of p0-357 300mg/L, it was observed that there is leaf dish to expand, lighter goes out in blade edge Existing callus.By the treatment of two weeks, leaf dish is transferred to and contains 6- benzyls aminoadenine 2.0mg/L, methyl α-naphthyl acetate 0.5mg/ Screening and culturing is done on the MS culture mediums of L, kanamycins 50mg/L and cephalosporin 300mg/L.After cultivating 3 weeks, in blade edge Substantial amounts of Multiple Buds are generated on the callus of edge.Treat that Multiple Buds grow into 2cm or so, be moved into containing 100mg/L ammonia Root induction on the MS culture mediums of benzyl mycin.Carry out practicing seedling after two weeks, be transplanted to after three days in flowerpot and grown.
The seed of the results of the tobacco after above-mentioned Agrobacterium is infected is collected, in the MS culture mediums containing 100mg/L ammonia benzyl mycins On screened, selection can be cultivated into soil with the seedling of normal growth, maturation after sowing son, continue containing 100mg/L cards, that is mould The MS Screening of Media of element.
Taking positive seeds carries out Molecular Identification, determines the expression of NtZFP8 genes.Result is as shown in Figure 3, it was demonstrated that this Invention obtains the homozygote of genes of interest NtZFP8 genes stabilization expression really.
The amino acid sequence (SEQ ID NO.2) of the albumen of NtZFP8 gene expressions is：
MDKTDRETRDFMNVESFSQLPFIRPAPANKEKAIRLFGKEFGTAGDSMAATESTETNPSQEEIKDHISN NSESSRKFECHYCCRNFPTSQALGGHQNAHKRERQNAKRAHLQSAMVHGALTEANIYGIMNYHRLGSAPTAAYHHHY ATNNINSATRFYGSHHHVNSTPYNSHQTPINGSPLALWRIPAATSVHHTSSSSSNFGRERSMNMQPLPVFAANNEDF KPSPIINSSSSQRGFGYETKAGIKDHVSLDLHL
Beneficial effects of the present invention are illustrated below by way of the mode of test example：
The NtZFP8 genes of test example 1 directly improve the ability of plant explant differentiation efficiency
1st, test method
By wild-type tobacco seed and NtZFP8 overexpressions tobacco seed, (embodiment 1 prepares the kind containing NtZFP8 genes Son), respectively with sterilization in sodium hypochlorite (1%) 4 minutes, as being dried in sterilizing filter paper, it is put in and contains MS solid mediums Sprouted on flat board.After 7 days, it is transferred in the blake bottle containing MS solid mediums and cultivates, after 15 days, breaks up for explant and try Test.
Wild type and NtZFP8 overexpression tobacco the 3rd blade of aseptic seedling are taken respectively, obtain equal big with 1cm card punch Vanelets, as induction is carried out on the flat board containing MS solid mediums, observe differentiation efficiency and obtain situation after 20d.
2nd, result of the test
Result shows, after the induction of wild-type tobacco leaf dish, has bud point to occur, but cannot grow up, it is impossible to obtain exploitation value The regeneration bud of value；After NtZFP8 overexpression tobacco leaf disc inductions, start bud point occur after 7 days, bud point can progressively grow up, reach More than 3cm, obtains the regeneration bud with value.
Result of the test explanation, after NtZFP8 genes of the present invention are transferred to tobacco, can improve the differentiation capability of tobacco explant.
The NtZFP8 genes of test example 2 improve the ability of plant callus differentiation efficiency
Leaf dish is obtained according to the methods described of test example 1, is placed in and is contained 6- benzyl aminoadenine 2.0mg/L and methyl α-naphthyl acetate Cultivated on the MS culture mediums of 0.5mg/L.After 10 days, statistics callus differentiation situation and bud are counted out；After 15 days, statistics Regrowth obtains number.
Result shows that, as shown in figure 4, the positive tobacco leaf callus for obtaining is produced early, surface bud shape projection is more, The regrowth number of acquisition is significantly higher than wild-type tobacco.
Result of the test explanation, after NtZFP8 genes of the present invention are transferred to tobacco, can improve the differentiation potency of tobacco healing tissue Power.
To sum up, the present invention has prepared NtZFP8 genes and recombinant bacterium, is transferred in plant, can effectively improve The explant of plant and the differentiation efficiency of callus, and then plant tissue culture technique efficiency is improved, application prospect is good.
SEQUENCE LISTING
<110>Sichuan University
<120>It is a kind of to turn the method that NtZFP8 genes improve Plant Tissue Breeding differentiation efficiency
<130> GY007-17P1102
<160> 4
<170> PatentIn version 3.5
<210> 1
<211> 771
<212> DNA
<213>The nucleotide sequence of NtZFP8 genes
<400> 1
atggacaaaa cagacagaga aactcgtgat ttcatgaacg tggagtcctt ctctcagcta 60
ccctttattc gaccagcacc agctaataaa gaaaaagcca ttaggctttt tggcaaagaa 120
tttggtactg ctggtgactc catggctgca acagaatcca ctgaaacaaa ccctagccaa 180
gaagaaatca aagaccatat tagcaacaac agtgagagca gtcgaaaatt cgagtgccat 240
tattgttgca ggaatttccc gacttcacaa gcactaggag gacatcaaaa tgctcataag 300
agagaacgcc agaatgctaa aagagcgcat cttcaatctg caatggtaca cggtgcttta 360
acagaggcaa atatttatgg aataatgaat taccatcgtc ttggtagtgc gccaacagca 420
gcttatcatc atcactacgc aaccaacaat attaacagtg ctactaggtt ttatggaagc 480
catcaccatg ttaatagtac cccttataat tctcatcaaa ctcctataaa tggaagtcct 540
ttagctttat ggaggattcc tgcagcaact agtgttcatc atacttcttc gtcttcgtct 600
aattttggcc gtgaacgatc catgaatatg cagccattgc cagtatttgc tgctaataat 660
gaggatttca agccttctcc aatcatcaat agctcaagtt ctcaaagagg gttcggatat 720
gaaacaaagg ccggaattaa agatcatgtg agtttagatc tacacttgtg a 771
<210> 2
<211> 256
<212> PRT
<213>The amino acid sequence of the albumen of NtZFP8 gene expressions
<400> 2
Met Asp Lys Thr Asp Arg Glu Thr Arg Asp Phe Met Asn Val Glu Ser
1 5 10 15
Phe Ser Gln Leu Pro Phe Ile Arg Pro Ala Pro Ala Asn Lys Glu Lys
20 25 30
Ala Ile Arg Leu Phe Gly Lys Glu Phe Gly Thr Ala Gly Asp Ser Met
35 40 45
Ala Ala Thr Glu Ser Thr Glu Thr Asn Pro Ser Gln Glu Glu Ile Lys
50 55 60
Asp His Ile Ser Asn Asn Ser Glu Ser Ser Arg Lys Phe Glu Cys His
65 70 75 80
Tyr Cys Cys Arg Asn Phe Pro Thr Ser Gln Ala Leu Gly Gly His Gln
85 90 95
Asn Ala His Lys Arg Glu Arg Gln Asn Ala Lys Arg Ala His Leu Gln
100 105 110
Ser Ala Met Val His Gly Ala Leu Thr Glu Ala Asn Ile Tyr Gly Ile
115 120 125
Met Asn Tyr His Arg Leu Gly Ser Ala Pro Thr Ala Ala Tyr His His
130 135 140
His Tyr Ala Thr Asn Asn Ile Asn Ser Ala Thr Arg Phe Tyr Gly Ser
145 150 155 160
His His His Val Asn Ser Thr Pro Tyr Asn Ser His Gln Thr Pro Ile
165 170 175
Asn Gly Ser Pro Leu Ala Leu Trp Arg Ile Pro Ala Ala Thr Ser Val
180 185 190
His His Thr Ser Ser Ser Ser Ser Asn Phe Gly Arg Glu Arg Ser Met
195 200 205
Asn Met Gln Pro Leu Pro Val Phe Ala Ala Asn Asn Glu Asp Phe Lys
210 215 220
Pro Ser Pro Ile Ile Asn Ser Ser Ser Ser Gln Arg Gly Phe Gly Tyr
225 230 235 240
Glu Thr Lys Ala Gly Ile Lys Asp His Val Ser Leu Asp Leu His Leu
245 250 255
<210> 3
<211> 23
<212> DNA
<213> NtZFP8-F
<400> 3
atggacaaaa cagacagaga aac 23
<210> 4
<211> 26
<212> DNA
<213> NtZFP8-R
<400> 4
tcacaagtgt agatctaaac tcacat 26

Claims (10)

1. a kind of genetic fragment, it is characterised in that：Its nucleotide sequence is as shown in SEQ ID NO.1.
2. a kind of recombinant vector, it is characterised in that：It includes the nucleotide sequence shown in SEQ ID NO.1.
3. recombinant vector according to claim 2, it is characterised in that：The recombinant vector is that restructuring pCAMBIA1301 is carried Body.
4. a kind of recombinant bacterium, it is characterised in that：It includes the recombinant vector described in claim 3 or 4.
5. recombinant bacterium according to claim 2, it is characterised in that：The recombinant bacterium is recombinational agrobacterium, is preferably recombinated Agrobacterium EHA105.
6. a kind of albumen, it is characterised in that：Its amino acid sequence is as shown in SEQ ID NO.2.
7. genetic fragment described in claim 1~6 any one, recombinant vector, recombinant bacterium, albumen are improving the explant of plant Purposes in the differentiation efficiency of body and/or callus.
8. purposes according to claim 7, it is characterised in that：The plant is tobacco.
9. it is a kind of improve Plant Tissue Breeding differentiation efficiency method, it is characterised in that：Take claim 1~5 any one institute Genetic fragment, recombinant vector, the recombinant bacterium stated, are transferred in plant, obtain stabilization expression claim 6 described in albumen plant or Person's seed, you can.
10. method according to claim 9, it is characterised in that：The plant is tobacco.
CN201710154117.9A 2017-03-15 2017-03-15 It is a kind of to turn the method that NtZFP8 genes improve Plant Tissue Breeding differentiation efficiency Pending CN106801062A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108795948A (en) * 2017-06-08 2018-11-13 四川大学 A method of turning NtZFP8 genes and improves rice tissue culture differentiation efficiency
CN114606259A (en) * 2022-01-27 2022-06-10 陕西师范大学 Application of CLE small peptide in controlling bud regeneration and as plant tissue culture proliferation regulator

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940159A (en) * 2010-06-30 2011-01-12 中国科学院华南植物园 Molecular breeding method for quickly obtaining large number of new transgenic Saussurea involucrata varieties
CN103421120A (en) * 2013-08-23 2013-12-04 中国农业科学院作物科学研究所 Application of rice transcription factor Os11g02540 genes

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101940159A (en) * 2010-06-30 2011-01-12 中国科学院华南植物园 Molecular breeding method for quickly obtaining large number of new transgenic Saussurea involucrata varieties
CN103421120A (en) * 2013-08-23 2013-12-04 中国农业科学院作物科学研究所 Application of rice transcription factor Os11g02540 genes

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GENBANK: "XM_009615426.2", 《GENBANK》 *
宋冰等: "植物C2H2型锌指蛋白的研究进展", 《基因组学与应用生物学》 *
杨明磊等: "烟草C2H2锌指蛋白转录因子家族成员的鉴定与表达分析", 《遗传》 *
王保艳等: "《生物学科知识与教学能力 高级中学 最新版》", 31 August 2016 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108795948A (en) * 2017-06-08 2018-11-13 四川大学 A method of turning NtZFP8 genes and improves rice tissue culture differentiation efficiency
CN114606259A (en) * 2022-01-27 2022-06-10 陕西师范大学 Application of CLE small peptide in controlling bud regeneration and as plant tissue culture proliferation regulator
CN114606259B (en) * 2022-01-27 2024-02-13 陕西师范大学 Application of CLE small peptides in controlling bud regeneration and serving as plant tissue culture proliferation regulator

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