CN106799268B - Compensation liquid separation operating method for pipettor - Google Patents
Compensation liquid separation operating method for pipettor Download PDFInfo
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- CN106799268B CN106799268B CN201710062089.8A CN201710062089A CN106799268B CN 106799268 B CN106799268 B CN 106799268B CN 201710062089 A CN201710062089 A CN 201710062089A CN 106799268 B CN106799268 B CN 106799268B
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- liquid separation
- volume
- pipettor
- drain
- time
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/02—Burettes; Pipettes
- B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/02—Identification, exchange or storage of information
- B01L2300/025—Displaying results or values with integrated means
Abstract
The present invention relates to pipettor technical fields, the more particularly, to compensation liquid separation operating method of pipettor, comprising the following steps: A, obtains Δ V in advanceIt is first、ΔVIt is residual；B, imbibition operation is carried out: setting pipettor imbibition volume VIt inhales, VIt inhales=liquid separation total volume VA+ΔVIt is first+ΔVIt is residual, then make pipettor imbibition；C, the 1st liquid separation operation: setting the 1st drain volume V of pipettor is carried out1, the 1st drain volume=1st time liquid separation volume V1+ΔVIt is first, then make pipettor drain；D, the 2nd time~n-th liquid separation operation: setting pipettor secondary drain volume, the secondary drain volume=secondary liquid separation volume V is successively carried outi, then make pipettor drain；Wherein；E, residual liquid is emptied, the present invention is by increasing Δ VIt is first、ΔVIt is residualIt compensates and corrects, guarantees liquid separation for the first time and last time liquid separation is all accurately, to improve liquid separation precision, reduce the wasting of resources, reduce experimental cost.
Description
Technical field:
The present invention relates to pipettor technical field more particularly to a kind of compensation liquid separation operating methods for pipettor.
Background technique:
In medical treatment, drug, gene and protein research, biological study, drug development laboratories and other biotechnologys
In application field, it is often necessary to manipulate laboratory in various laboratory procedures using liquid metering devices such as pipettors
Fluid sample, if pipettor is that liquid is then distributed into one or more points in the liquid sucking pipette by certain volume
With volume.Existing pipettor carry out liquid separation mode process in, start imbibition when and when drain for the first time, due to pipettor inner cavity
Air pressure and external pressure there are pressure difference so that the volume of practical imbibition can the imbibition volume less than normal in setting, drain for the first time
Actual volume also can the liquid separation volume less than normal for the first time in setting, in addition, pipettor inner cavity has liquid after last time liquid separation
Residual, i.e., the actual volume of last time drain can the last time liquid separation volume less than normal in setting.Therefore existing pipettor point
Liquid separation for the first time when liquid, last time liquid separation can all have an error, and people can will liquid separation liquid for the first time when using pipettor liquid separation
Body, last time liquid separation body abandon, and cause the waste of resource, especially biology, many liquid reagents of pharmaceuticals industry are very precious
, considerably increase the cost of experiment.
Summary of the invention:
One kind is provided the purpose of the present invention is to the shortcomings of the prior art can be improved liquid separation precision, reduces
The wasting of resources, the compensation liquid separation operating method for pipettor for reducing experimental cost.
To achieve the goals above, the technical solution adopted by the present invention is that:
Compensation liquid separation operating method for pipettor, comprising the following steps:
A, compensation rate volume delta V when the 1st liquid separation is obtained ahead of timeIt is first, liquid remaining balance body after last time liquid separation
Product Δ VIt is residual；
B, imbibition operation is carried out: setting pipettor imbibition volume VIt inhales, VIt inhales=liquid separation total volume VA+ΔVIt is first+ΔVIt is residual, then make
Pipettor imbibition；
C, the 1st liquid separation operation: setting the 1st drain volume of pipettor, the 1st drain volume=1st time liquid separation is carried out
Volume V1+ΔVIt is first, then make pipettor drain；
D, the 2nd time~n-th liquid separation is successively carried out to operate: setting pipettor secondary drain volume, the secondary drain volume=
The secondary liquid separation volume Vi, then make pipettor drain；Wherein, n is liquid separation total degree, and n is natural number greater than 1, i be no more than
The natural number of n；
E, residual liquid is emptied.
Pass through the compensation of head drop of liquid when measurement and calculating the 1st liquid separation of acquisition in the step A by the way of experiment
Measure volume delta VIt is first, liquid remaining balance volume delta V after last time liquid separationIt is residual。
Preferably, the step A specifically:
A1, the operation of liquid separation each time, each drain volume of the pipettor=secondary liquid separation are successively carried out by uncompensated mode
Volume；
A2, measurement obtain practical drain volume, the practical drain volume of last time liquid separation of the 1st liquid separation；
A3, the 1st liquid separation volume compares with the practical drain volume of the 1st liquid separation calculates difference, which is
For Δ VIt is first；Last time liquid separation volume is compared with the practical drain volume of last time liquid separation and calculates difference, the difference
As Δ VIt is residual。
It can also be previously according to Δ V corresponding to different air pressures, temperature in the step AIt is first、ΔVIt is residualData are made in numerical value
Table, liquid separation operation by tables of data obtain corresponding Δ V according to air pressure at that time, temperatureIt is first、ΔVIt is residualNumerical value.
After completing a pipetting, automatic Memory is when time operating process and parameter and returns to start-up operation state, when
When pipetting does not change operating process and parameter setting next time, automatically by the operating process and ginseng of last pipetting
Number is arranged to the operating process and parameter of pipetting next time.
The beneficial effects of the invention are that: the present invention is used for the compensation liquid separation operating method of pipettor, comprising the following steps:
A, compensation rate volume delta V when the 1st liquid separation is obtained ahead of timeIt is first, liquid remaining balance volume delta V after last time liquid separationIt is residual；B,
Carry out imbibition operation: setting pipettor imbibition volume VIt inhales, VIt inhales=liquid separation total volume VA+ΔVIt is first+ΔVIt is residual, then inhale pipettor
Liquid；C, the 1st liquid separation operation: setting the 1st drain volume of pipettor, the 1st drain volume=1st time liquid separation volume V is carried out1
+ΔVIt is first, then make pipettor drain；D, the 2nd time~n-th liquid separation operation: setting pipettor secondary drain volume is successively carried out,
The secondary drain volume=secondary liquid separation volume Vi, then make pipettor drain；Wherein；E, residual liquid is emptied, the present invention passes through
Increase compensation rate volume delta VIt is first1st liquid separation volume is compensated and corrected, by increasing liquid remaining balance volume delta VIt is residualIt is right
Last time liquid separation volume compensates and corrects, and guarantees liquid separation for the first time and last time liquid separation is all accurately, to improve liquid separation essence
Degree reduces the wasting of resources, reduces experimental cost.
Specific embodiment:
Below with reference to embodiment, the present invention is further illustrated, the compensation liquid separation operation side for pipettor
Method, the compensation liquid separation operating method for pipettor, comprising the following steps:
One, compensation rate volume delta V when the 1st liquid separation is obtained ahead of timeIt is first, liquid remaining balance body after last time liquid separation
Product Δ VIt is residual；
Two, imbibition operation is carried out: setting pipettor imbibition volume VIt inhales, VIt inhales=liquid separation total volume VA+ΔVIt is first+ΔVIt is residual, then
Make pipettor imbibition；
Three, the 1st liquid separation operation: setting the 1st drain volume of pipettor, the 1st drain volume=1st time liquid separation is carried out
Volume V1+ΔVIt is first, then make pipettor drain；
Four, the 2nd time~n-th liquid separation is successively carried out to operate: setting pipettor secondary drain volume, the secondary drain volume=
The secondary liquid separation volume Vi, then make pipettor drain；Wherein, n is liquid separation total degree, and n is natural number greater than 1, i be no more than
The natural number of n；Specifically:
Set the 2nd drain volume of pipettor, the 2nd drain volume=2nd time liquid separation volume V2, then arrange pipettor
Liquid；
Set the 3rd drain volume of pipettor, the 3rd drain volume=3rd time liquid separation volume V3, then arrange pipettor
Liquid；
…………
Set pipettor n-th drain volume, n-th drain volume=n-th liquid separation volume Vn, then arrange pipettor
Liquid；
Five, residual liquid is emptied.
The compensation of head drop of liquid when can obtain the 1st liquid separation by measurement and calculating by the way of experiment in step 1
Measure volume delta VIt is first, liquid remaining balance volume delta V after last time liquid separationIt is residual, specifically:
1, the operation of liquid separation each time, each drain volume of the pipettor=secondary liquid separation body are successively carried out by uncompensated mode
Product；
2, measurement obtains practical drain volume, the practical drain volume of last time liquid separation of the 1st liquid separation；
3, the 1st liquid separation volume is compared with the practical drain volume of the 1st liquid separation and calculates difference, which is
ΔVIt is first；Last time liquid separation volume is compared with the practical drain volume of last time liquid separation and calculates difference, which is
For Δ VIt is residual。
It can also be previously according to Δ V corresponding to different air pressures, temperature in step 1It is first、ΔVIt is residualTables of data is made in numerical value, point
Liquid operation by tables of data obtains corresponding Δ V according to air pressure at that time, temperatureIt is first、ΔVIt is residualNumerical value.
After completing a pipetting, automatic Memory is when time operating process and parameter and returns to start-up operation state, when
When pipetting does not change operating process and parameter setting next time, automatically by the operating process and ginseng of last pipetting
Number is arranged to the operating process and parameter of pipetting next time.
The present invention is by increasing compensation rate volume delta VIt is first1st liquid separation volume is compensated and corrected, by increasing liquid
Remaining balance volume delta VIt is residualLast time liquid separation volume is compensated and corrected, guarantees liquid separation for the first time and last time liquid separation is all
Accurately, liquid separation precision is improved, the wasting of resources is reduced, reduces experimental cost.
Certainly, the above is only presently preferred embodiments of the present invention, therefore all according to structure described in present patent application range
It makes, the equivalent change or modification that feature and principle are done, is included in the scope of the patent application of the present invention.
Claims (3)
1. being used for the compensation liquid separation operating method of pipettor, which comprises the following steps:
A, in advance by the way of experiment by measuring and calculating compensation rate volume delta V when obtaining the 1st liquid separationIt is first, last
Liquid remaining balance volume delta V after secondary liquid separationIt is residual；Specifically:
A1, the operation of liquid separation each time, each drain volume of the pipettor=secondary liquid separation volume are successively carried out by uncompensated mode；
A2, measurement obtain practical drain volume, the practical drain volume of last time liquid separation of the 1st liquid separation；
A3, the 1st liquid separation volume compares with the practical drain volume of the 1st liquid separation calculates difference, which is Δ
VIt is first；Last time liquid separation volume is compared with the practical drain volume of last time liquid separation and calculates difference, which is
ΔVIt is residual；
B, imbibition operation is carried out: setting pipettor imbibition volume VIt inhales, VIt inhales=liquid separation total volume VA+ΔVIt is first+ΔVIt is residual, then make liquid relief
Device imbibition；
C, the 1st liquid separation operation: setting the 1st drain volume of pipettor, the 1st drain volume=1st time liquid separation volume V is carried out1
+ΔVIt is first, then make pipettor drain；
D, the 2nd time~n-th liquid separation operation: setting pipettor secondary drain volume, secondary drain volume=this time is successively carried out
Liquid separation volume Vi, then make pipettor drain；Wherein, n is liquid separation total degree, and n is the natural number greater than 1, and i is no more than n's
Natural number；
E, residual liquid is emptied.
2. the compensation liquid separation operating method according to claim 1 for pipettor, it is characterised in that: the step A
In previously according to Δ V corresponding to different air pressures, temperatureIt is first、ΔVIt is residualTables of data is made in numerical value, and liquid separation operation is according to gas at that time
Pressure, temperature obtain corresponding Δ V by tables of dataIt is first、ΔVIt is residualNumerical value.
3. the compensation liquid separation operating method of pipettor is used for described in -2 any one according to claim 1, it is characterised in that:
After completing a pipetting, automatic Memory is when time operating process and parameter and returns to start-up operation state, moves when next time
Liquid operation without changing operating process and when parameter setting, automatically by the operating process of last pipetting and parameter setting at
The operating process and parameter of pipetting next time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710062089.8A CN106799268B (en) | 2017-01-31 | 2017-01-31 | Compensation liquid separation operating method for pipettor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710062089.8A CN106799268B (en) | 2017-01-31 | 2017-01-31 | Compensation liquid separation operating method for pipettor |
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| Publication Number | Publication Date |
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| CN106799268A CN106799268A (en) | 2017-06-06 |
| CN106799268B true CN106799268B (en) | 2019-06-04 |
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| CN201710062089.8A Active CN106799268B (en) | 2017-01-31 | 2017-01-31 | Compensation liquid separation operating method for pipettor |
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| CN115770631B (en) * | 2022-12-07 | 2023-08-15 | 深圳市恒永达科技股份有限公司 | Environment-adaptive air type liquid transfer pump and control method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7770475B2 (en) * | 2007-06-29 | 2010-08-10 | Rainin Instrument, Llc | Hybrid manual-electronic pipette |
| CN103008040B (en) * | 2012-09-05 | 2015-08-05 | 罗耿荣 | The electric pipettor of auto modification precision and precision correcting method thereof |
| CN103495442B (en) * | 2013-10-16 | 2015-12-23 | 佛山市顺德区罗恩科学仪器有限公司 | Electric pipettor and automatic gauge method, automatic liquid separation method |
| CN103480441B (en) * | 2013-10-16 | 2015-08-05 | 佛山市顺德区罗恩科学仪器有限公司 | Auxiliary aspirator |
| CN203620655U (en) * | 2013-10-16 | 2014-06-04 | 佛山市顺德区罗恩科学仪器有限公司 | Electric pipette |
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