The electric pipettor of auto modification precision and precision correcting method thereof
Technical field:
The present invention relates to pipettor technical field, particularly relate to a kind of electric pipettor and precision correcting method thereof of auto modification precision.
Background technology:
In medical treatment, medicine, gene and protein research, biological study, drug development laboratories and other biological technology application, often need to utilize pipettor to carry out handle laboratory in various laboratory procedures, it utilizes pipettor to be sucked in pipette by the liquid of certain volume, then Liquid distribution is become one or more dispensed volume.
Existing pipettor generally comprises processor, drive unit and piston, and processor is according to the volume that will move liquid, and accessory drive driven plunger moves respective distance h, and the actual liquid that moves amasss V and is:
V=π R
2h, wherein R is the radius of piston.
But pipettor is owing to being subject to the impact of environment and operation technique, the actual liquid that moves accumulates in trueness error, and the principal element affecting precision is as follows:
1. temperature
The Accuracy of temperature to pipettor is larger, the change of temperature directly can cause the change of volume, because the air in pipettor cannot adaptive temperature be significantly or unexpected change enough rapidly, before pipettor operation, in order to ensure precision, need to carry out constant temperature process, and desirable room temperature is (21.5+1) DEG C, inconvenient operation.
2. pressure
The foundation of the design principle of pipettor is " Hooke principle ", its operation principle is that piston realizes imbibition and tapping by the stretching motion of spring, deaeration under piston promotes, atmospheric pressure is utilized to suck liquid, promote air by piston again and discharge liquid, the size of air pressure directly affects the volume of imbitition, and the volume of often piston movement must be larger than desired volume aspirated.Therefore, pressure has become the factor that impact is larger.
3. the angle of imbibition
Suction nozzle enters liquid level and horizontal plane requirement as far as possible close to upright position, otherwise vertical fluid column is less, can suck too much liquid like this, and impact moves liquid precision.
Existing pipettor antijamming capability is weak, the impact being easily subject to above-mentioned factor make precision and reliability low.
Summary of the invention:
Object of the present invention is exactly the electric pipettor of the deficiency that exists for prior art and the auto modification precision that provides a kind of precision high, also provides the precision correcting method of this electric pipettor.
To achieve these goals, the technical solution used in the present invention is:
The electric pipettor of auto modification precision, it includes microprocessor, the liquid that moves moving liquid volumetric quantities to microprocessor input amasss input module, the drive motors module of driven plunger movement, move the input that liquid amasss input module connection microprocessor, the input of drive motors module connects the output of microprocessor, also include temperature detecting module, air pressure detection module, temperature detecting module is connected the input of microprocessor respectively with the output of air pressure detection module, microprocessor receive move liquid amass input module input move liquid volumetric quantities after calculate piston should the distance of movement, simultaneously by temperature detecting module, air pressure detection module detects the temperature change value of environment respectively in real time, air pressure change value also feeds back to microprocessor and should the distance of movement revise piston.
The present invention also comprises gyro sensor, and the output of gyro sensor connects the input of microprocessor, and spiral shell instrument sensor detects the deviation angle of electric pipettor in real time and feeds back to microprocessor and should the distance of movement revise piston.
The present invention also comprises position detecting module, and the output of position detecting module connects the input of microprocessor, and position detecting module detects the position of electric pipettor in real time and feeds back to microprocessor and should the distance of movement revise piston.
The present invention also comprises load detection of loss of mains module, and the output of load detection of loss of mains module connects the input of microprocessor, and load detection of loss of mains module detects the step loss condition of motor in real time and feeds back to microprocessor and should the distance of movement revise piston.
Present invention also offers the precision correcting method of electric pipettor, comprise the following steps:
A, to microprocessor input move liquid volumetric quantities;
B, microprocessor according to move liquid volumetric quantities calculate piston should the distance of movement;
C, the temperature change value detecting environment at least respectively in real time, air pressure change value feed back to microprocessor and should the distance of movement revise piston, draw the revised displacement of piston;
D, move corresponding distance according to piston revised displacement control piston.
Also detect the deviation angle of electric pipettor in step C in real time and feed back to microprocessor piston should the distance of movement be revised.
Also detect the position of electric pipettor in step C in real time and feed back to microprocessor piston should the distance of movement be revised.
Also detect the step loss condition of motor in step C in real time and feed back to microprocessor piston should the distance of movement be revised.
Beneficial effect of the present invention is: electric pipettor of the present invention includes microprocessor, move liquid and amass input module, the drive motors module of driven plunger movement, temperature detecting module, air pressure detection module, temperature detecting module is connected the input of microprocessor respectively with the output of air pressure detection module, microprocessor receive move liquid amass input module input move liquid volumetric quantities after calculate piston should the distance of movement, the present invention passes through temperature detecting module, air pressure detection module detects the temperature change value of environment respectively in real time, air pressure change value also feeds back to microprocessor and automatically should the distance of movement revise piston, control piston moves corresponding distance, improve and move liquid control accuracy, the antijamming capability of the raising stability of a system and to external world environment, reduce system cost.
Accompanying drawing illustrates:
Fig. 1 is principle schematic of the present invention.
Detailed description of the invention:
Below in conjunction with accompanying drawing, the present invention is further illustrated, as shown in Figure 1, the electric pipettor of auto modification precision, it includes microprocessor 1, the liquid that moves moving liquid volumetric quantities to microprocessor 1 input amasss input module 2, the drive motors module 3 of driven plunger 4 movement, temperature detecting module 5, air pressure detection module 6, gyro sensor 7, position detecting module 8, load detection of loss of mains module 9, move liquid and amass input module 2 for man-machine interface, move liquid and amass the input that input module 2 connects microprocessor 1, the input of drive motors module 3 connects the output of microprocessor 1.Temperature detecting module 5 is connected the input of microprocessor 1 respectively with the output of air pressure detection module 6, microprocessor 1 receive move that liquid amasss that input module 2 inputs move liquid volumetric quantities after calculate piston 4 should the distance of movement, detect the temperature change value of environment, air pressure change value feed back to microprocessor 1 pair of piston 4 and should the distance of movement revise respectively in real time by temperature detecting module 5, air pressure detection module 6 simultaneously.
Drive motors module 3 comprises stepper motor and stepper motor driver, command adapted thereto is sent to stepper motor driver control step motor movement by microprocessor 1, promote piston 4 by stepper motor again to move, the accurate single axial movement of piston 4 produces mattress effect (piston 4 stroke) and is drawn onto in suction nozzle by liquid.Support that stepper motor micro-stepping segmentation function has half step, 1/4,1/8,1/16, this function ensure that the smooth motion of stepper motor and very little torque fluctuations, and several segmentation pattern can be selected in user oneself.Motor have passed through zero point with linear, and when through zero crossing, the torque fluctuations of motor is very little.
Temperature detecting module 5 has temperature detecting function, when the temperature varies, temperature detecting module 5 can receive the change of ambient temperature delicately, and then feed back to microprocessor 1, being driven by drive motors module 3 makes piston 4 make corresponding displacement correction in time, and the distance that we claim this to revise is real-time modifying factor α.
Temperature detecting module 5 can also play the effect of protection motor, and the essence of thermal protection for motors is the overtemperature prote that stator winding runs maximum temperature position.If using the mean temperature of stator winding as the criterion of Thermal protection; even if the detection of its mean temperature is accurate and non-overtemperature; but maximum temperature may overtemperature stator winding local may overtemperature in other words, thus cause stator winding minor insulation to damage, cause whole motor damage.Controller judges whether to break down after gathering current signal, if there is fault, provides alarm signal according to fault type.If belong to stall fault, then directly cut off the electricity supply, avoid harm to expand; If belong to the fault such as overload, three-phase imbalance (comprising disconnected phase), overvoltage, under-voltage, temporarily do not take safeguard measure; then judge whether temperature transfinites; as temperature does not exceed permissible value; then only provide alarm signal and do not cut off the electricity supply; farthest to play the effect of motor, reduce and even avoid production process to interrupt the economic loss brought.Once maximum temperature overtemperature, protector cuts off motor power immediately.
Air pressure detection module 6 has air pressure measuring ability, detect the air pressure change value of environment in real time and feed back to microprocessor 1 pair of piston 4 and should the distance of movement revise, this correction value is counted in real-time modifying factor α, even if atmospheric pressure changes, motor driver also can drive piston 4 that corresponding variation occurs.
The output of gyro sensor 7 connects the input of microprocessor 1, utilize the gyroscopic inertia of gyro sensor 7 and the characteristic of precession, spiral shell instrument sensor detects the deviation angle of electric pipettor and piston 4 distance of stretch out and draw back in real time and feeds back to microprocessor 1 pair of piston 4 and should the distance of movement revise, and this correction value is counted in real-time modifying factor α.
The output of position detecting module 8 connects the input of microprocessor 1, there is position sensing function, without the need to external reference point, the Aligning control of external reference point is not needed to move, position detecting module 8 detects the position of electric pipettor in real time and feeds back to microprocessor 1 pair of piston 4 and should the distance of movement revise, and this correction value is counted in real-time modifying factor α.
The output of load detection of loss of mains module 9 connects the input of microprocessor 1, load detection of loss of mains module 9 detects the step loss condition of motor in real time and feeds back to microprocessor 1 pair of piston 4 and should the distance of movement revise, and this correction value is counted in real-time modifying factor α.Load detection of loss of mains module 9 can detect initial point, stroke automatically, and what can solve stepper motor loses step problem, ensures that piston 4 stroke is consistent with numerical value.
Present invention also offers the precision correcting method of above-mentioned electric pipettor, comprise the following steps:
A, to microprocessor 1 input move liquid volumetric quantities;
B, microprocessor 1 according to move liquid volumetric quantities calculate piston 4 should the distance h of movement;
The deviation angle of C, the temperature change value detecting environment respectively in real time, air pressure change value, electric pipettor, the position of electric pipettor, the step loss condition of motor feed back to microprocessor 1 and draw real-time modifying factor α, then according to real-time modifying factor α, piston 4 distance of movement should be carried out to correction and draw the revised displacement of piston 4 (h+ α);
D, move corresponding distance according to piston 4 revised displacement control piston 4.
The actual liquid that moves drawn by said method amasss V and is:
V=πR
2(h+α)
Wherein, R is the radius of piston 4, and h is that piston 4 should the distance of movement, and α is real-time modifying factor.
External environment and operation technique can be offset on the impact of pipettor precision by real-time modifying factor α, and can according to the change auto modification precision of external environment and operation technique
The present invention is by temperature detecting module 5, air pressure detection module 6, gyro sensor 7, position detecting module 8, load detection of loss of mains module 9 detects the temperature change value of environment respectively in real time, air pressure change value, the deviation angle of electric pipettor, the position of electric pipettor, the step loss condition of motor also feeds back to microprocessor 1 and automatically should the distance of movement revise piston 4, external environment and operation technique can be offset on the impact of pipettor precision by real-time modifying factor α, and can according to the change auto modification precision of external environment and operation technique, control piston 4 moves corresponding distance, improve and move liquid control accuracy, the antijamming capability of the raising stability of a system and to external world environment, reduce system cost.The requirement of the environment for use of electric pipettor can be reduced by the present invention, keep precision, to reduce the interference of external environment condition by the self-recision of inside, thus ensure that the precision of pipettor, person easy to use, thus the misgivings decreasing user, break the limitation of environment for use.
Certainly, the above is only preferred embodiment of the present invention, therefore all equivalences done according to structure, feature and the principle described in patent claim of the present invention change or modify, and are included in patent claim of the present invention.